Pub Date : 2023-05-30DOI: 10.15575/biodjati.v8i1.24856
D. F. Kusharyati, Fira Julian Ariaputri, H. Pramono, Anwar Rovik
Mangrove is a unique ecosystem. Only a few studies have explored the presence of lactic acid bacteria and their roles in mangrove ecosystems. From mangrove sediments at Logending Beach in Jawa Tengah (Indonesia), the Lactic Acid Bacteria (LAB) isolates LG-50, LG-107, and LG-114 were discovered. They produce bacteriocins. This study aimed to determine the characteristics of LAB isolates, antimicrobial activity, and physicochemical properties of bacteriocins. LAB isolates were characterized by morphology, physiology, and biochemistry. The production of bacteriocin was performed by salting-out method, followed by testing its antimicrobial activity against pathogenic bacteria. Isolates LG-50, LG-107, and LG-114 are thought to be in the Lactobacillus group. The crude bacteriocin can inhibit the growth of Gram-positive and negative bacteria. The average inhibition zones against Escherichia coli and Staphylococcus aureus were 16.67 mm and 22.17 mm, respectively. The crude bacteriocin tested positive for ninhydrin. It confirmed the crude bacteriocin was a protein and sensitive to the proteolytic enzyme. SDS-PAGE analysis presented the molecular weight of crude bacteriocin was 38 kDa. This present study supports the potential use of bacteriocin in the pharmaceutical and food industries.
{"title":"Lactic Acid Bacteria from Mangrove Sediment Produce Bacteriocins Active Against Gram-Positive and Negative Bacteria","authors":"D. F. Kusharyati, Fira Julian Ariaputri, H. Pramono, Anwar Rovik","doi":"10.15575/biodjati.v8i1.24856","DOIUrl":"https://doi.org/10.15575/biodjati.v8i1.24856","url":null,"abstract":"Mangrove is a unique ecosystem. Only a few studies have explored the presence of lactic acid bacteria and their roles in mangrove ecosystems. From mangrove sediments at Logending Beach in Jawa Tengah (Indonesia), the Lactic Acid Bacteria (LAB) isolates LG-50, LG-107, and LG-114 were discovered. They produce bacteriocins. This study aimed to determine the characteristics of LAB isolates, antimicrobial activity, and physicochemical properties of bacteriocins. LAB isolates were characterized by morphology, physiology, and biochemistry. The production of bacteriocin was performed by salting-out method, followed by testing its antimicrobial activity against pathogenic bacteria. Isolates LG-50, LG-107, and LG-114 are thought to be in the Lactobacillus group. The crude bacteriocin can inhibit the growth of Gram-positive and negative bacteria. The average inhibition zones against Escherichia coli and Staphylococcus aureus were 16.67 mm and 22.17 mm, respectively. The crude bacteriocin tested positive for ninhydrin. It confirmed the crude bacteriocin was a protein and sensitive to the proteolytic enzyme. SDS-PAGE analysis presented the molecular weight of crude bacteriocin was 38 kDa. This present study supports the potential use of bacteriocin in the pharmaceutical and food industries.","PeriodicalId":17683,"journal":{"name":"Jurnal Biodjati","volume":"28 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139372459","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-30DOI: 10.15575/biodjati.v8i1.22884
Imadiah Aulia, Muta Ali Khalifa, Ani Rahmawati, Muhammad Syauqi, Mila Wahyuningsih, Syifa Jihan Raimahua, Wilda Akmalia
Blue swimming crab (BSC) (Portunus pelagicus) is the main fisheries commodity in Banten Bay, with the highest catch of catch occurring in the west monsoon. Increased fishing efforts have reduced the catch, as indicated by a decrease in the average size of carapace width. Overfishing might influence the life cycle and population structure of the BSC. This research aims to analyze the growth and mortality of the Blue Swimming Crab in Banten Bay, especially in the west monsoon. Blue swimming crab samples were obtained from the catch of fish trap and gillnet fishermen with landing sites around the Karangantu Archipelago Fishing Port. Growth, age, and mortality were predicted based on the measurement of total carapace width and body weight. Data were analyzed using Fisat II and Microsoft Excel. The frequency distribution of carapace showed one mode class which normally was distributed consisting of only one age structure group (cohort). The relationship between carapace width and weight of males or females resulted in a slope value (b>3) and was positive allometric, indicating that both sexes had a faster weight gain than the increase in carapace width. Growth parameters resulted asymptotic length (L∞); male 176.75 mm and female 176.46 mm. The growth was relatively fast, with a growth coefficient value of more than one. The mortality showed that the fishing mortality value (F);in males was 3.47 /year and in females 2.69/year was greater than the natural mortality value (M); males 1.25/year and females 1.22/year. Blue Swimming Crabs in Banten Bay had overfished as shown by the high fishing mortality value and there were still many BSC that were caught below the legal size allowed. Thus, catches should be controlled to keep the population and for sustainable fisheries.
蓝游蟹（BSC）（Portunus pelagicus）是万丹湾的主要渔业商品，在西季风季节捕获量最高。捕捞强度的增加导致捕获量减少，这表现在平均胴体宽度的减小上。过度捕捞可能会影响 BSC 的生命周期和种群结构。本研究旨在分析万丹湾蓝游蟹的生长和死亡率，尤其是在西季风季节。蓝游蟹样本取自卡朗安图群岛渔港附近上岸地点的诱捕器和刺网渔民的渔获物。根据胴体总宽度和体重的测量结果预测其生长、年龄和死亡率。数据使用 Fisat II 和 Microsoft Excel 进行分析。腕部的频率分布显示出一个模式类，该模式类呈正态分布，仅由一个年龄结构组（群组）组成。雄性或雌性腕宽与体重之间的关系产生了一个斜率值（b>3），且呈正异速关系，这表明雌雄鱼的体重增长速度均快于腕宽的增长速度。生长参数得出的渐近长度（L∞）为：雄性 176.75 毫米，雌性 176.46 毫米。生长速度相对较快，生长系数值大于 1。死亡率显示，捕捞死亡率（F）雄性为 3.47/年，雌性为 2.69/年，高于自然死亡率（M）雄性为 1.25/年，雌性为 1.22/年。从高渔捞死亡率值可以看出，万丹湾的蓝游蟹已被过度捕捞，仍有许多蓝游蟹的捕捞量低于法定允许的大小。因此，应控制渔获量，以保持种群数量和渔业的可持续发展。
{"title":"Age Structure, Growth, and Mortality of Blue Swimming Crab (Portunus pelagicus Linnaeus,1758) in Banten Bay Waters","authors":"Imadiah Aulia, Muta Ali Khalifa, Ani Rahmawati, Muhammad Syauqi, Mila Wahyuningsih, Syifa Jihan Raimahua, Wilda Akmalia","doi":"10.15575/biodjati.v8i1.22884","DOIUrl":"https://doi.org/10.15575/biodjati.v8i1.22884","url":null,"abstract":"Blue swimming crab (BSC) (Portunus pelagicus) is the main fisheries commodity in Banten Bay, with the highest catch of catch occurring in the west monsoon. Increased fishing efforts have reduced the catch, as indicated by a decrease in the average size of carapace width. Overfishing might influence the life cycle and population structure of the BSC. This research aims to analyze the growth and mortality of the Blue Swimming Crab in Banten Bay, especially in the west monsoon. Blue swimming crab samples were obtained from the catch of fish trap and gillnet fishermen with landing sites around the Karangantu Archipelago Fishing Port. Growth, age, and mortality were predicted based on the measurement of total carapace width and body weight. Data were analyzed using Fisat II and Microsoft Excel. The frequency distribution of carapace showed one mode class which normally was distributed consisting of only one age structure group (cohort). The relationship between carapace width and weight of males or females resulted in a slope value (b>3) and was positive allometric, indicating that both sexes had a faster weight gain than the increase in carapace width. Growth parameters resulted asymptotic length (L∞); male 176.75 mm and female 176.46 mm. The growth was relatively fast, with a growth coefficient value of more than one. The mortality showed that the fishing mortality value (F);in males was 3.47 /year and in females 2.69/year was greater than the natural mortality value (M); males 1.25/year and females 1.22/year. Blue Swimming Crabs in Banten Bay had overfished as shown by the high fishing mortality value and there were still many BSC that were caught below the legal size allowed. Thus, catches should be controlled to keep the population and for sustainable fisheries.","PeriodicalId":17683,"journal":{"name":"Jurnal Biodjati","volume":"24 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139372497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-30DOI: 10.15575/biodjati.v8i1.23826
Titanio Auditya Pribadi, Mufidah Afiyanti, Luchman Hakim
Agroforestry is a form of sustainable agriculture; the present threat of agroforestry is a change in agroforestry landforms that have switched to a less sustainable form. Coffee plays a role in the agroforestry ecosystem, Kalibaru is one of the Banyuwangi District coffee centers managed by the local community. The objective of this study was to determine the vegetation structure and analyze the effect of that on environmental parameters. Data was collected by vegetation analysis and soil parameter analysis with PAST 2020 Software version 4.05. In total, 15 research plots were used, consisting of 5 complex agroforestry, 5 simple agroforestry, and 5 monoculture plots. The results showed 20 species in complex agroforestry, 24 species in simple agroforestry, and 18 species in monoculture were found. The highest important index level in complex agroforestry: Durio zibethinus 63.87% for trees, the Swietenia mahagoni 64.43% for poles, and Coffee canephora 128.87% for shrubs. In simple agroforestry : Cocos nucifera 72.83% for trees, Leucaena leucocephala 80.70% for poles, and the Coffea canephora 137.57% for shrubs. In monoculture agroforestry: Hibiscus similis 82.99% for trees, Leucaena leucocephala 209.22% for poles, and Coffea canephora 130.96% for shrubs. Agroforestry in Kalibaru was categorized in the moderate diversity. The diversity index (H') in complex agroforestry showed H' trees 2.21, pole 2.03 and. shrubs 1.60. Simple agroforestry shows H' tree 2.00, pole 2.49, and shrubs 1.50. Monoculture shows H' tree 1.98, pole 0.64, and shrubs 1.54. Agroforestry a role in maintaining soil moisture and temperature, complex agroforestry: temperature 24 0C, and Moist 5.82, simple: temperature 24.2 0C, Moist 5.42, and Monoculture temperature 26 0C, Moist 4.54.
{"title":"Vegetation Structure and Composition of Coffee Agroforestry in Kalibaru District","authors":"Titanio Auditya Pribadi, Mufidah Afiyanti, Luchman Hakim","doi":"10.15575/biodjati.v8i1.23826","DOIUrl":"https://doi.org/10.15575/biodjati.v8i1.23826","url":null,"abstract":"Agroforestry is a form of sustainable agriculture; the present threat of agroforestry is a change in agroforestry landforms that have switched to a less sustainable form. Coffee plays a role in the agroforestry ecosystem, Kalibaru is one of the Banyuwangi District coffee centers managed by the local community. The objective of this study was to determine the vegetation structure and analyze the effect of that on environmental parameters. Data was collected by vegetation analysis and soil parameter analysis with PAST 2020 Software version 4.05. In total, 15 research plots were used, consisting of 5 complex agroforestry, 5 simple agroforestry, and 5 monoculture plots. The results showed 20 species in complex agroforestry, 24 species in simple agroforestry, and 18 species in monoculture were found. The highest important index level in complex agroforestry: Durio zibethinus 63.87% for trees, the Swietenia mahagoni 64.43% for poles, and Coffee canephora 128.87% for shrubs. In simple agroforestry : Cocos nucifera 72.83% for trees, Leucaena leucocephala 80.70% for poles, and the Coffea canephora 137.57% for shrubs. In monoculture agroforestry: Hibiscus similis 82.99% for trees, Leucaena leucocephala 209.22% for poles, and Coffea canephora 130.96% for shrubs. Agroforestry in Kalibaru was categorized in the moderate diversity. The diversity index (H') in complex agroforestry showed H' trees 2.21, pole 2.03 and. shrubs 1.60. Simple agroforestry shows H' tree 2.00, pole 2.49, and shrubs 1.50. Monoculture shows H' tree 1.98, pole 0.64, and shrubs 1.54. Agroforestry a role in maintaining soil moisture and temperature, complex agroforestry: temperature 24 0C, and Moist 5.82, simple: temperature 24.2 0C, Moist 5.42, and Monoculture temperature 26 0C, Moist 4.54.","PeriodicalId":17683,"journal":{"name":"Jurnal Biodjati","volume":"55 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139372542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-30DOI: 10.15575/biodjati.v8i1.23764
Wisnu Eka Wardana, Dea Hastaning Tantri, Marshanda Nur Roosyana Afifah, Hasna Nur Aini, D. Siswanti, Khusnul Qonita Maghfiroh, T. Erfi̇anti̇, Ria Amelia, D. Kurnianto, E. Suyono
One way to reduce the adverse effects of the heavy metals mercury in the aquatic environment are using organisms to break down or convert toxic substances into non-toxic forms, either by phytore- mediation or phycoremediation. This research aimed to analyze the growth and lipid content of Euglena sp. after mercury exposure. This research also aimed to analyze the growth of E. palaefolius which is associated with Euglena sp. In this study, the bioremediation ability of Euglena sp. and Echinodorus palaefolius through treatment with mercury concentrations of 5 ppm, 10 ppm, 15 ppm, and 20 ppm, as well as association and non-association treatments. The parameters are the growth of Euglena sp. and the association between Euglena sp. andE. palaefolius measurement and lipid content. The result of the growth of Euglena sp. experienced a significant increase. Lipid content in Euglena sp. was also seen high at 10 ppm mercury concentration. In E. palaefolius, the ability to adsorb heavy metals was also shown by the large diameter of the stems and also the plant growth which has optimal growth in the treatment of 10 ppm mercury stress.
{"title":"Effect of Mercury Stress on the Growth and Lipid Content of Euglena sp. and Echinodorus palaefolius","authors":"Wisnu Eka Wardana, Dea Hastaning Tantri, Marshanda Nur Roosyana Afifah, Hasna Nur Aini, D. Siswanti, Khusnul Qonita Maghfiroh, T. Erfi̇anti̇, Ria Amelia, D. Kurnianto, E. Suyono","doi":"10.15575/biodjati.v8i1.23764","DOIUrl":"https://doi.org/10.15575/biodjati.v8i1.23764","url":null,"abstract":"One way to reduce the adverse effects of the heavy metals mercury in the aquatic environment are using organisms to break down or convert toxic substances into non-toxic forms, either by phytore- mediation or phycoremediation. This research aimed to analyze the growth and lipid content of Euglena sp. after mercury exposure. This research also aimed to analyze the growth of E. palaefolius which is associated with Euglena sp. In this study, the bioremediation ability of Euglena sp. and Echinodorus palaefolius through treatment with mercury concentrations of 5 ppm, 10 ppm, 15 ppm, and 20 ppm, as well as association and non-association treatments. The parameters are the growth of Euglena sp. and the association between Euglena sp. andE. palaefolius measurement and lipid content. The result of the growth of Euglena sp. experienced a significant increase. Lipid content in Euglena sp. was also seen high at 10 ppm mercury concentration. In E. palaefolius, the ability to adsorb heavy metals was also shown by the large diameter of the stems and also the plant growth which has optimal growth in the treatment of 10 ppm mercury stress.","PeriodicalId":17683,"journal":{"name":"Jurnal Biodjati","volume":"107 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139372685","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-30DOI: 10.15575/biodjati.v8i1.20689
M. Dwiati, Salma Auliya Rahmah, Agus Hery Susanto
Cogongrass is one of the top ten problem-causing weeds in the world that is invasively spread over many tropical and subtropical regions, including Java Island, the Republic of Indonesia. The wide distribution is possibly related to their adaptability to almost all terrestrial habitats from various altitudes, which may lead to a high level of genetic variation. This study aimed to assess the genetic variation and phylogenetic relationship of cogongrass in Java Island using an intergenic spacer (IGS) sequence in the cpDNA genome, i.e. trnT(UGU)-trnL(UAA), as the molecular marker of high mutation rate. Plant samples were collected randomly from five different sites on the island. These were subjected to total genomic DNA extraction. The DNAs obtained were then used as PCR templates to amplify trnT(UGU)-trnL(UAA) IGS using a pair of universal primers. The PCR products were sequenced for genetic analysis. It was shown that an extremely high genetic variation with respect to the haplotype diversity of cogongrass in Java Island was observed. Nevertheless, a very low nucleotide diversity was obtained revealing a considerably close genetic relationship among the samples. Anatomical data regarding stomata number and length were found to correspond to the high level of genetic variation, but it was not the case with the stomata index.
Cogongrass是世界十大问题杂草之一，入侵性地分布在许多热带和亚热带地区，包括印度尼西亚共和国的爪哇岛。其广泛分布可能与其对不同海拔高度几乎所有陆生栖息地的适应性有关，这可能导致其遗传变异水平较高。 本研究旨在利用 cpDNA 基因组中的基因间间隔序列（IGS），即 trnT(UGU)-trnL(UAA)，作为高突变率的分子标记，评估爪哇岛狗牙根草的遗传变异和系统发育关系。从岛上五个不同地点随机采集了植物样本。对这些样本进行总基因组 DNA 提取。然后将获得的 DNA 用作 PCR 模板，使用一对通用引物扩增 trnT(UGU)-trnL(UAA) IGS。对 PCR 产物进行测序，以进行遗传分析。结果表明，在爪哇岛观察到的猪笼草单倍型多样性具有极高的遗传变异。然而，核苷酸多样性非常低，这表明样本之间的遗传关系相当密切。关于气孔数量和长度的解剖学数据被发现与高水平的遗传变异相对应，但气孔指数却并非如此。
{"title":"Molecular Profile of Cogongrass [Imperata cylindrica (L.) Raeusch] in Java Island Based on trnT(UGU)-trnL(UAA) IGS Sequences","authors":"M. Dwiati, Salma Auliya Rahmah, Agus Hery Susanto","doi":"10.15575/biodjati.v8i1.20689","DOIUrl":"https://doi.org/10.15575/biodjati.v8i1.20689","url":null,"abstract":"Cogongrass is one of the top ten problem-causing weeds in the world that is invasively spread over many tropical and subtropical regions, including Java Island, the Republic of Indonesia. The wide distribution is possibly related to their adaptability to almost all terrestrial habitats from various altitudes, which may lead to a high level of genetic variation. This study aimed to assess the genetic variation and phylogenetic relationship of cogongrass in Java Island using an intergenic spacer (IGS) sequence in the cpDNA genome, i.e. trnT(UGU)-trnL(UAA), as the molecular marker of high mutation rate. Plant samples were collected randomly from five different sites on the island. These were subjected to total genomic DNA extraction. The DNAs obtained were then used as PCR templates to amplify trnT(UGU)-trnL(UAA) IGS using a pair of universal primers. The PCR products were sequenced for genetic analysis. It was shown that an extremely high genetic variation with respect to the haplotype diversity of cogongrass in Java Island was observed. Nevertheless, a very low nucleotide diversity was obtained revealing a considerably close genetic relationship among the samples. Anatomical data regarding stomata number and length were found to correspond to the high level of genetic variation, but it was not the case with the stomata index.","PeriodicalId":17683,"journal":{"name":"Jurnal Biodjati","volume":"112 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139372422","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-30DOI: 10.15575/biodjati.v8i1.24521
A. Kusumorini, N. Hasna, Yani Suryani, Opik Taufiqurrahman4
Currently, Covid-19 has become endemic. However, the development of Covid-19 drugs continues to be carried out to suppress the growth of the Sars-Cov-2 virus. Some compounds with antiviral activity are catechin, galangin, and hesperidin. Angiotensin-converting enzyme-2 (ACE-2) is a protein that enters viruses into the cell. Based on that, ACE-2 can be used as a primary target to suppress the development of the Sars-Cov-2 virus. This study aimed to test the catechin, galangin, and hesperidin compounds in inhibiting the SARS CoV-2 virus from attaching to ACE-2 by trying the interactions of catechin, galangin, and hesperidin compounds with ACE-2 using the in-silico method. The material used was the three-dimensional structure of the compounds catechin, galangin hesperidin, and ACE-2. The tools used were FAF-Drugs4, Discovery Studio, and Pyrex software. Low-affinity energy values (kcal/mol) indicate promising results. The results showed that the energy affinity value of catechin was -6.2 kcal/mol, galangin was -6.3 kcal/mol, and hesperidin was -8.3 kcal/mol. This value is lower than the control affinity energy (chloroquine and favipiravir), which is -5.2 kcal/mol and -4.8 kcal/mol, respectively. Based on this, catechin, galangin, and hesperidin can be used as inhibitors/competitors for the Sars-Cov-2 to attach to ACE-2.
{"title":"In Silico Analysis of Catechin, Galangin, and Hesperidin as Competitors of the SARS-CoV-2 Spike Protein","authors":"A. Kusumorini, N. Hasna, Yani Suryani, Opik Taufiqurrahman4","doi":"10.15575/biodjati.v8i1.24521","DOIUrl":"https://doi.org/10.15575/biodjati.v8i1.24521","url":null,"abstract":"Currently, Covid-19 has become endemic. However, the development of Covid-19 drugs continues to be carried out to suppress the growth of the Sars-Cov-2 virus. Some compounds with antiviral activity are catechin, galangin, and hesperidin. Angiotensin-converting enzyme-2 (ACE-2) is a protein that enters viruses into the cell. Based on that, ACE-2 can be used as a primary target to suppress the development of the Sars-Cov-2 virus. This study aimed to test the catechin, galangin, and hesperidin compounds in inhibiting the SARS CoV-2 virus from attaching to ACE-2 by trying the interactions of catechin, galangin, and hesperidin compounds with ACE-2 using the in-silico method. The material used was the three-dimensional structure of the compounds catechin, galangin hesperidin, and ACE-2. The tools used were FAF-Drugs4, Discovery Studio, and Pyrex software. Low-affinity energy values (kcal/mol) indicate promising results. The results showed that the energy affinity value of catechin was -6.2 kcal/mol, galangin was -6.3 kcal/mol, and hesperidin was -8.3 kcal/mol. This value is lower than the control affinity energy (chloroquine and favipiravir), which is -5.2 kcal/mol and -4.8 kcal/mol, respectively. Based on this, catechin, galangin, and hesperidin can be used as inhibitors/competitors for the Sars-Cov-2 to attach to ACE-2.","PeriodicalId":17683,"journal":{"name":"Jurnal Biodjati","volume":"101 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139372563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Lauraceae is a group of tropical plants with a wide distribution. The abundance and species richness of the Lauraceae family includes the under to the upper canopy of the forest. The Lauraceae family can be utilized in various sectors, especially economically. Limiting characters as a significance in the Lauraceae classification was needed to provide additional information regarding taxon grouping. This research aimed to study epidermal characteristics, leaf venation variations, and the diversity of Lauraceae species under different forest environmental conditions. The research procedure involved: collecting ecological field data and morphological identification using a purposive sampling method based on the presence of species. Soil sampling and environmental microclimate were measured on each plot. The anatomical process was carried out by recording characters. Correlation between environmental factors and plant targets used canonical correspondence analysis. The kinship between members of Lauraceae was expressed in a dendrogram. The result of this study showed that Lauraceae could live at various elevations. The analysis data indicated differences in environmental factors that affect the existence of Lauraceae species. Leaf venation studies support clustering in the family using characters such as primary, secondary, tertiary veins, and areola. Observation of leaf venation can be used as a taxonomic significance in the family Lauraceae. Epidermal cell walls in leaf samples are straight, curved shallow, deep, and sharp. Other characters that can be found are anomocytic, laterocytic, and brachyparasitic types of stomata, with stellate and glandless trichomes The anatomical dendrogram separated Lauraceae into clusters on a similarity coefficient scale.
{"title":"Diversity and Epidermal Characteristic of Lauraceae Leaf in Two Forest Locations, Bogor Regency, West Java","authors":"Nurul Amalia Fadhila, Sulistijorini Sulistijorini, Nina Ratna Djuita","doi":"10.15575/biodjati.v8i1.24406","DOIUrl":"https://doi.org/10.15575/biodjati.v8i1.24406","url":null,"abstract":"Lauraceae is a group of tropical plants with a wide distribution. The abundance and species richness of the Lauraceae family includes the under to the upper canopy of the forest. The Lauraceae family can be utilized in various sectors, especially economically. Limiting characters as a significance in the Lauraceae classification was needed to provide additional information regarding taxon grouping. This research aimed to study epidermal characteristics, leaf venation variations, and the diversity of Lauraceae species under different forest environmental conditions. The research procedure involved: collecting ecological field data and morphological identification using a purposive sampling method based on the presence of species. Soil sampling and environmental microclimate were measured on each plot. The anatomical process was carried out by recording characters. Correlation between environmental factors and plant targets used canonical correspondence analysis. The kinship between members of Lauraceae was expressed in a dendrogram. The result of this study showed that Lauraceae could live at various elevations. The analysis data indicated differences in environmental factors that affect the existence of Lauraceae species. Leaf venation studies support clustering in the family using characters such as primary, secondary, tertiary veins, and areola. Observation of leaf venation can be used as a taxonomic significance in the family Lauraceae. Epidermal cell walls in leaf samples are straight, curved shallow, deep, and sharp. Other characters that can be found are anomocytic, laterocytic, and brachyparasitic types of stomata, with stellate and glandless trichomes The anatomical dendrogram separated Lauraceae into clusters on a similarity coefficient scale.","PeriodicalId":17683,"journal":{"name":"Jurnal Biodjati","volume":"135 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139372682","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-30DOI: 10.15575/biodjati.v8i1.20634
N. R. Prasetiawan, R. Kurniasih, Putri Milenia Damayanti, Muhammad Agus
The mangrove ecosystem is one of the accumulation areas for various marine debris including plastic and also the habitat for gastropods. This condition allows direct contact between gastropods and debris which can be an entranceway for contamination in biota. This study aimed to know the species of gastropods in the marine debris in the Bulaksetra mangrove ecosystem in Pangandaran. The method used was stratified random sampling with a transect line of 100 m. Gastropods and debris in the 1 m x 1 m square in the 10 m x 10 m plot were collected, identified, counted, and determined in composition. There were 22 types of marine debris in the Bulaksetra mangrove ecosystem which were dominated by plastic. 16 species of gastropods were found in the area around the debris and 15 species were found attached to the debris. Gastropods can be found on the top, middle, or bottom surfaces of plastic waste. Faunus ater and Terebralia palustris dominate in the surrounding area and the debris. Treefauna gastropods utilize stuck debris to move from one part to another part of the tree. Plastic waste has become part of the mangrove habitat and there are interactions with gastropods that use it as a living medium.
{"title":"Gastropods on Marine Debris at Mangrove Ecosystem","authors":"N. R. Prasetiawan, R. Kurniasih, Putri Milenia Damayanti, Muhammad Agus","doi":"10.15575/biodjati.v8i1.20634","DOIUrl":"https://doi.org/10.15575/biodjati.v8i1.20634","url":null,"abstract":"The mangrove ecosystem is one of the accumulation areas for various marine debris including plastic and also the habitat for gastropods. This condition allows direct contact between gastropods and debris which can be an entranceway for contamination in biota. This study aimed to know the species of gastropods in the marine debris in the Bulaksetra mangrove ecosystem in Pangandaran. The method used was stratified random sampling with a transect line of 100 m. Gastropods and debris in the 1 m x 1 m square in the 10 m x 10 m plot were collected, identified, counted, and determined in composition. There were 22 types of marine debris in the Bulaksetra mangrove ecosystem which were dominated by plastic. 16 species of gastropods were found in the area around the debris and 15 species were found attached to the debris. Gastropods can be found on the top, middle, or bottom surfaces of plastic waste. Faunus ater and Terebralia palustris dominate in the surrounding area and the debris. Treefauna gastropods utilize stuck debris to move from one part to another part of the tree. Plastic waste has become part of the mangrove habitat and there are interactions with gastropods that use it as a living medium.","PeriodicalId":17683,"journal":{"name":"Jurnal Biodjati","volume":"371 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139372704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-30DOI: 10.15575/biodjati.v8i1.25015
I. P. G. Damayanto, H. Rustiami, M. Miftahudin, T. Chikmawati
More than 40 species of bamboo were known from the Lesser Sunda Islands (LSI), including several endemic species. Endemic species are often highly specialized, found in limited geographical areas, and vulnerable to extinction if their habitat is destroyed. This study aimed to provide an account of endemic bamboo species on the LSI. Fieldwork involved collecting samples of bamboo in the LSI. Herbarium specimens of bamboo deposited in the Herbarium Bogoriense (BO), collected from the LSI were examined, and endemic LSI species were recorded. The conservation status followed the International Union for Conservation of Nature (IUCN) red list categories and criteria. The conservation status of unassessed species was evaluated using IUCN red list categories and criteria. A spatial analysis was also conducted. Data were analyzed and presented descriptively. There are 10 endemic bamboo species in the LSI, namely Bambusa ooh Widjaja & Astuti from Bali (conservation status: endangered), Chloothamnus reholttumianus (Soenarko) Widjaja from Sumba (vulnerable), C. schmutzii (S.Dransf.) Widjaja from Sumbawa and Flores (near threatened), Dinochloa kostermansiana S.Dransf. from Sumba and Flores (near threatened), D. sepang Widjaja & Astuti from Bali (vulnerable), Fimbribambusa rifaiana Widjaja from Alor (near threatened), Gigantochloa aya Widjaja & Astuti from Bali (vulnerable), G. baliana Widjaja & Astuti from Bali (vulnerable), G. taluh Widjaja & Astuti from Bali (vulnerable), and Schizostachyum purpureum Damayanto & Widjaja from Sumba (data deficient). They can be found at an altitude of 20–1700 m in various habitats, including forests, bamboo forests, villages, botanical gardens, along a road, slopes, valleys, riverbanks, and around a lake.
{"title":"Endemic Bamboo (Poaceae, Bambusoideae) of the Lesser Sunda Islands","authors":"I. P. G. Damayanto, H. Rustiami, M. Miftahudin, T. Chikmawati","doi":"10.15575/biodjati.v8i1.25015","DOIUrl":"https://doi.org/10.15575/biodjati.v8i1.25015","url":null,"abstract":"More than 40 species of bamboo were known from the Lesser Sunda Islands (LSI), including several endemic species. Endemic species are often highly specialized, found in limited geographical areas, and vulnerable to extinction if their habitat is destroyed. This study aimed to provide an account of endemic bamboo species on the LSI. Fieldwork involved collecting samples of bamboo in the LSI. Herbarium specimens of bamboo deposited in the Herbarium Bogoriense (BO), collected from the LSI were examined, and endemic LSI species were recorded. The conservation status followed the International Union for Conservation of Nature (IUCN) red list categories and criteria. The conservation status of unassessed species was evaluated using IUCN red list categories and criteria. A spatial analysis was also conducted. Data were analyzed and presented descriptively. There are 10 endemic bamboo species in the LSI, namely Bambusa ooh Widjaja & Astuti from Bali (conservation status: endangered), Chloothamnus reholttumianus (Soenarko) Widjaja from Sumba (vulnerable), C. schmutzii (S.Dransf.) Widjaja from Sumbawa and Flores (near threatened), Dinochloa kostermansiana S.Dransf. from Sumba and Flores (near threatened), D. sepang Widjaja & Astuti from Bali (vulnerable), Fimbribambusa rifaiana Widjaja from Alor (near threatened), Gigantochloa aya Widjaja & Astuti from Bali (vulnerable), G. baliana Widjaja & Astuti from Bali (vulnerable), G. taluh Widjaja & Astuti from Bali (vulnerable), and Schizostachyum purpureum Damayanto & Widjaja from Sumba (data deficient). They can be found at an altitude of 20–1700 m in various habitats, including forests, bamboo forests, villages, botanical gardens, along a road, slopes, valleys, riverbanks, and around a lake.","PeriodicalId":17683,"journal":{"name":"Jurnal Biodjati","volume":"11 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139372475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-30DOI: 10.15575/biodjati.v8i1.19331
W. Rahajeng, J. Restuono, F. C. Indriani
Information about the morphological and genetic characteristics of sweet potato (Ipomoea batatas) is important in the variety development program. This experiment aimed to determine the diversity and genetic relationship of sweet potato accessions from NTT based on morphological character data. The research was carried out at Kendalpayak Research Station, Malang, East Java from April - September 2019. The materials used were seventy-one sweet potato germplasm from the ILETRI collection (collected from NTT). Each accession was planted on a 1 m × 5 m plot size, with a spacing of 100 × 20 cm (single row). Fertilization was carried out using a dose of “100 kg urea + 100 kg SP36 + 200 kg KCl ha-1”. The observed characters were the vines length, growth type, internode length, leaf length, leaf width, leaf size, leaf bone color, leaf shape, leaf characteristics, lobes number, lobe shape, mature leaf color, shoot color, pigmentation of petiole, young stems pigmentation (dominant and secondary color), young leaves feathers, tuber skin color, tuber flesh color, weight of canopy, number of tubers perplot, weight of tubers perplot, number and weight of tubers perplot. Cluster analysis was carried out using the Minitab 17 program. There was morphological diversity in seventy-one accessions of sweet potato germplasm from NTT. Principal component analysis resulted in seven main components with the proportion of diversity 76.3%. cluster analysis, seventy-one accessions of sweet potato germplasm were divided into fifteen accession groups on the basis of 80% degree. Characteristics of shoot color, mature leaf color, leaf size, petiole pigmentation, and leaf bone color contributed greatly to the total diversity.
{"title":"The Relationship of Sweet Potato Germplasm Based on Morphological Characters","authors":"W. Rahajeng, J. Restuono, F. C. Indriani","doi":"10.15575/biodjati.v8i1.19331","DOIUrl":"https://doi.org/10.15575/biodjati.v8i1.19331","url":null,"abstract":"Information about the morphological and genetic characteristics of sweet potato (Ipomoea batatas) is important in the variety development program. This experiment aimed to determine the diversity and genetic relationship of sweet potato accessions from NTT based on morphological character data. The research was carried out at Kendalpayak Research Station, Malang, East Java from April - September 2019. The materials used were seventy-one sweet potato germplasm from the ILETRI collection (collected from NTT). Each accession was planted on a 1 m × 5 m plot size, with a spacing of 100 × 20 cm (single row). Fertilization was carried out using a dose of “100 kg urea + 100 kg SP36 + 200 kg KCl ha-1”. The observed characters were the vines length, growth type, internode length, leaf length, leaf width, leaf size, leaf bone color, leaf shape, leaf characteristics, lobes number, lobe shape, mature leaf color, shoot color, pigmentation of petiole, young stems pigmentation (dominant and secondary color), young leaves feathers, tuber skin color, tuber flesh color, weight of canopy, number of tubers perplot, weight of tubers perplot, number and weight of tubers perplot. Cluster analysis was carried out using the Minitab 17 program. There was morphological diversity in seventy-one accessions of sweet potato germplasm from NTT. Principal component analysis resulted in seven main components with the proportion of diversity 76.3%. cluster analysis, seventy-one accessions of sweet potato germplasm were divided into fifteen accession groups on the basis of 80% degree. Characteristics of shoot color, mature leaf color, leaf size, petiole pigmentation, and leaf bone color contributed greatly to the total diversity.","PeriodicalId":17683,"journal":{"name":"Jurnal Biodjati","volume":"55 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139372513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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