Pub Date : 2021-11-29DOI: 10.15575/biodjati.v6i2.12393
S. Azizah, Mikhania Christiningtyas Eryani, A. Azizah
Probiotics are microbes in fermented foods that have beneficial effects on health. Microbes that act as probiotics are lactic acid bacteria (LAB) that can produce metabolites such as lactic acid, hydrogen peroxide, and bacteriocins. This study aimed to obtain lactic acid bacterial isolates from tape and tempeh, and to test the potential of LAB as a probiotic candidate by activity test as an antidiarrhea and its resistance to gastric pH and bile salts. The fermentation products used as a source of LAB isolates are tempeh sumber mas merk, and yellow cassava tape, sari madu merk from Jember. The results of the first stage regarding the isolation of LAB using GYP media showed that there were 2 LAB isolates (TaJ.14 and TaJ.15) from the tape and 4 LAB isolates (TeJ.18, TeJ.22, TeJ.24, and TeJ.25) from tempeh. The results of the antidiarrheal test using the disc diffusion method (oxoid) showed that TaJ.14 and TaJ.15 isolates were able to inhibit Bacillus subtilis, Escherichia coli, and Shigella dysentriae, while TeJ.18, TeJ.22, TeJ.24, TeJ.25, and Lactobacillus casei (control) was only able to inhibit B. subtilis and E. coli. The results of LAB resistance to gastric pH showed that the TeJ.25 isolate had the highest percentage of pH 3 and 2.5 resistance (51.13 and 33.03%) compared to other isolates and controls. LAB resistance test results against bile salts (oxgal) showed that the TeJ.22 isolate had the highest percentage of resistance (75.10%) compared to other isolates although was still higher in control (75.99%).
{"title":"Potential of Lactic Acid Bacteria From Tape and Jember Tempeh as a Probiotic Candidate","authors":"S. Azizah, Mikhania Christiningtyas Eryani, A. Azizah","doi":"10.15575/biodjati.v6i2.12393","DOIUrl":"https://doi.org/10.15575/biodjati.v6i2.12393","url":null,"abstract":"Probiotics are microbes in fermented foods that have beneficial effects on health. Microbes that act as probiotics are lactic acid bacteria (LAB) that can produce metabolites such as lactic acid, hydrogen peroxide, and bacteriocins. This study aimed to obtain lactic acid bacterial isolates from tape and tempeh, and to test the potential of LAB as a probiotic candidate by activity test as an antidiarrhea and its resistance to gastric pH and bile salts. The fermentation products used as a source of LAB isolates are tempeh sumber mas merk, and yellow cassava tape, sari madu merk from Jember. The results of the first stage regarding the isolation of LAB using GYP media showed that there were 2 LAB isolates (TaJ.14 and TaJ.15) from the tape and 4 LAB isolates (TeJ.18, TeJ.22, TeJ.24, and TeJ.25) from tempeh. The results of the antidiarrheal test using the disc diffusion method (oxoid) showed that TaJ.14 and TaJ.15 isolates were able to inhibit Bacillus subtilis, Escherichia coli, and Shigella dysentriae, while TeJ.18, TeJ.22, TeJ.24, TeJ.25, and Lactobacillus casei (control) was only able to inhibit B. subtilis and E. coli. The results of LAB resistance to gastric pH showed that the TeJ.25 isolate had the highest percentage of pH 3 and 2.5 resistance (51.13 and 33.03%) compared to other isolates and controls. LAB resistance test results against bile salts (oxgal) showed that the TeJ.22 isolate had the highest percentage of resistance (75.10%) compared to other isolates although was still higher in control (75.99%).","PeriodicalId":17683,"journal":{"name":"Jurnal Biodjati","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81996502","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-11-29DOI: 10.15575/biodjati.v6i2.13191
I. J. Sari, Indri Wahyuni, R. Khastini, Ewi Awaliyati, Andriana Susilowati, Enggar Utari, N. Aryantha
Plant Growth Promoting Bacteria Rhizobacteria (PGPR) is one of the potential bacteria to enhance of Capsicum annuum through inhabitation the growth of pathogenic fungi. This study aimed to characterize PGPR in chili plants (Capsicum annuum). PGPR was isolated from the soil habitat of the red chili plant in Cilegon, Indonesia. Screening was then carried out with the dual culture method on Petri dishes and tested through in vivo method on the red chili plant. The selected bacteria were characterized morphologically, biochemically, and physiologically. The results revealed that there were 14 single isolates of bacteria from the roots of the red chili plants. The five single bacterial isolates, namely Azostobacter, Azospirillum, Pseudomonas, Serratia, and Beijerinckia have good potential as PGPR based on multiple culture screening by producing clear zones and positively effect the growth of chili plants.
{"title":"Characterization of Plant Growth Promoting Rhizobacteria (PGPR) on Capcissum annum","authors":"I. J. Sari, Indri Wahyuni, R. Khastini, Ewi Awaliyati, Andriana Susilowati, Enggar Utari, N. Aryantha","doi":"10.15575/biodjati.v6i2.13191","DOIUrl":"https://doi.org/10.15575/biodjati.v6i2.13191","url":null,"abstract":"Plant Growth Promoting Bacteria Rhizobacteria (PGPR) is one of the potential bacteria to enhance of Capsicum annuum through inhabitation the growth of pathogenic fungi. This study aimed to characterize PGPR in chili plants (Capsicum annuum). PGPR was isolated from the soil habitat of the red chili plant in Cilegon, Indonesia. Screening was then carried out with the dual culture method on Petri dishes and tested through in vivo method on the red chili plant. The selected bacteria were characterized morphologically, biochemically, and physiologically. The results revealed that there were 14 single isolates of bacteria from the roots of the red chili plants. The five single bacterial isolates, namely Azostobacter, Azospirillum, Pseudomonas, Serratia, and Beijerinckia have good potential as PGPR based on multiple culture screening by producing clear zones and positively effect the growth of chili plants.","PeriodicalId":17683,"journal":{"name":"Jurnal Biodjati","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89588802","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-06-01DOI: 10.15575/BIODJATI.V6I1.10603
T. M. Fakih, D. Ramadhan, F. Darusman
The COVID-19 has spread worldwide and become an international pandemic. The promising target for drug discovery of COVID-19 was SARS-CoV-2 Main Protease (Mpro), that has been successfully crystallized along with its inhibitor. The discovery of peptide-based inhibitors may present better options than small molecules for inhibitor SARS-CoV-2 Mpro. Natural compounds have such a wide potential and still few explored, Zizyphus spina-christi is one of the medicinal plants that have many pharmacological activities and contains a peptide compound from alkaloids class, i.e. cyclopeptide alkaloids, that is interesting to explore as SARS-CoV-2 Mpro inhibitor. The compound structure was drawn and optimized using density functional theory 3-21G method. The protein chosen was the high resolution of SARS-CoV-2 MPro receptor (1.45 Å) with PDB ID: 6WNP, in complex with boceprevir. Molecular docking simulation was performed using Autodock4 with 100 numbers of GA run, the validation methods assessed by RMSD calculation. Furthermore, the prediction of pharmacological activity spectra was carried out using the PASS Prediction server. The results showed RMSD value was 1.98 Å, this docking method was valid. The binding energy of all compounds showed better results than the native ligand (Boceprevir). The in silico PASS prediction results indicated that all compounds showed antiviral activity. Some compounds showed protease inhibitory activity, i.e Ambiphibine-H, Franganine, and Mauritine-A, and the highest Pa (Predicted activity) value showed by Mauritine-A compounds. It can be concluded that the cyclopeptide compounds of Zizyphus spina-christi were indicated to have a potential as COVID-19 therapy targeting SARS-CoV-2 Mpro.
{"title":"In Silico Activity Identification of Cyclo Peptide Alkaloids from Zizyphus Spina-Christi Species Against Sars-Cov-2 Main Protease","authors":"T. M. Fakih, D. Ramadhan, F. Darusman","doi":"10.15575/BIODJATI.V6I1.10603","DOIUrl":"https://doi.org/10.15575/BIODJATI.V6I1.10603","url":null,"abstract":"The COVID-19 has spread worldwide and become an international pandemic. The promising target for drug discovery of COVID-19 was SARS-CoV-2 Main Protease (Mpro), that has been successfully crystallized along with its inhibitor. The discovery of peptide-based inhibitors may present better options than small molecules for inhibitor SARS-CoV-2 Mpro. Natural compounds have such a wide potential and still few explored, Zizyphus spina-christi is one of the medicinal plants that have many pharmacological activities and contains a peptide compound from alkaloids class, i.e. cyclopeptide alkaloids, that is interesting to explore as SARS-CoV-2 Mpro inhibitor. The compound structure was drawn and optimized using density functional theory 3-21G method. The protein chosen was the high resolution of SARS-CoV-2 MPro receptor (1.45 Å) with PDB ID: 6WNP, in complex with boceprevir. Molecular docking simulation was performed using Autodock4 with 100 numbers of GA run, the validation methods assessed by RMSD calculation. Furthermore, the prediction of pharmacological activity spectra was carried out using the PASS Prediction server. The results showed RMSD value was 1.98 Å, this docking method was valid. The binding energy of all compounds showed better results than the native ligand (Boceprevir). The in silico PASS prediction results indicated that all compounds showed antiviral activity. Some compounds showed protease inhibitory activity, i.e Ambiphibine-H, Franganine, and Mauritine-A, and the highest Pa (Predicted activity) value showed by Mauritine-A compounds. It can be concluded that the cyclopeptide compounds of Zizyphus spina-christi were indicated to have a potential as COVID-19 therapy targeting SARS-CoV-2 Mpro.","PeriodicalId":17683,"journal":{"name":"Jurnal Biodjati","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81090185","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-05-30DOI: 10.15575/biodjati.v5i1.5260
R. Rosidah, Y. Mulyani, W. Lili, Khasanatur Rosyidah
Common carp (Cyprinus carpio) cultivation is often hampered by a disease attack, one of them is the attack of Edwarsiella tarda. Lactic acid bacteria (LAB) can be used as an alternative to prevent diseases in fish by increasing the body's resistance. This research aimed to determine the most effective isolates of LAB that increase of the resistance of carp fingerlings to the attack of E. tarda bacteria and see which isolates can produce the highest survival. The LAB isolates used were the result of isolation from the gut of carp. This study used a Completely Randomized Design (CRD) consisting of four treatments with three replications. The fish were immersed with different LAB isolates, CcB7, CcB8, and CcB15 in the same density of 108 cells / mm3. Immersion was carried out for 30 minutes with a frequency of seven days. While during the research, two immersions were carried out before the challenge test against E. tarda bacteria. The parameters observed were the number of leukocytes, hematocrit, erythrocyte, differential leukocytes, survival rate, and clinical symptoms that appeared. The results showed that all LAB isolates used in this study could increase the body resistance of carp against the attacks of E. tarda bacteria. The LAB CcB7 isolate was the most effective for enhancing the body resistance of carp fish withthe highest increase level of leukocyte, erythrocyte, and hematocrit were 18 ± 0.057,7 ± 0,077, and 0.26± 7.31% respectively. After being challenged with E. tarda bacteria producing mild clinical symptoms, the highest increase is in monocyte and neutrophil cells was 20 and 62% respectively, the highest reduction in lymphocytes was – 9% and the highest survival rate was 80%.
{"title":"Effectiveness of Lactic Acid Bacteria to Improve Cyprinus carpio Fingerlings Resistance Against","authors":"R. Rosidah, Y. Mulyani, W. Lili, Khasanatur Rosyidah","doi":"10.15575/biodjati.v5i1.5260","DOIUrl":"https://doi.org/10.15575/biodjati.v5i1.5260","url":null,"abstract":"Common carp (Cyprinus carpio) cultivation is often hampered by a disease attack, one of them is the attack of Edwarsiella tarda. Lactic acid bacteria (LAB) can be used as an alternative to prevent diseases in fish by increasing the body's resistance. This research aimed to determine the most effective isolates of LAB that increase of the resistance of carp fingerlings to the attack of E. tarda bacteria and see which isolates can produce the highest survival. The LAB isolates used were the result of isolation from the gut of carp. This study used a Completely Randomized Design (CRD) consisting of four treatments with three replications. The fish were immersed with different LAB isolates, CcB7, CcB8, and CcB15 in the same density of 108 cells / mm3. Immersion was carried out for 30 minutes with a frequency of seven days. While during the research, two immersions were carried out before the challenge test against E. tarda bacteria. The parameters observed were the number of leukocytes, hematocrit, erythrocyte, differential leukocytes, survival rate, and clinical symptoms that appeared. The results showed that all LAB isolates used in this study could increase the body resistance of carp against the attacks of E. tarda bacteria. The LAB CcB7 isolate was the most effective for enhancing the body resistance of carp fish withthe highest increase level of leukocyte, erythrocyte, and hematocrit were 18 ± 0.057,7 ± 0,077, and 0.26± 7.31% respectively. After being challenged with E. tarda bacteria producing mild clinical symptoms, the highest increase is in monocyte and neutrophil cells was 20 and 62% respectively, the highest reduction in lymphocytes was – 9% and the highest survival rate was 80%. ","PeriodicalId":17683,"journal":{"name":"Jurnal Biodjati","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74766724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-05-30DOI: 10.15575/biodjati.v5i1.8111
D. Lam, A. Nguyen, Tung T. Nguyen
The Amynthas and Metaphire species recorded in Vietnam have been rechecked based on original descriptions and preserved specimens. As a result, Metaphire birmanica (Rosa, 1888) is recorded in Vietnam for the first time. The species is recognized by having three pairs of spermathecal pores in 5/6/7/8, male pores in xviii, presence of copulatory pouches, no genial markings, and manicate intestinal caeca. In addition, three species have been rechecked and re-assigned to different genera, namely Amynthas tripidoporophoratus (Thai & Nguyen, 1993) comb. nov., Metaphire dranfocana (Do & Huynh, 1993) comb. nov., Metaphire anhumalatana (Thai & Huynh, 1993) comb. nov.
{"title":"The First Record of Metaphire Birmanica (Rosa, 1888) in Vietnam, with Notes on Several Earthworm Species","authors":"D. Lam, A. Nguyen, Tung T. Nguyen","doi":"10.15575/biodjati.v5i1.8111","DOIUrl":"https://doi.org/10.15575/biodjati.v5i1.8111","url":null,"abstract":"The Amynthas and Metaphire species recorded in Vietnam have been rechecked based on original descriptions and preserved specimens. As a result, Metaphire birmanica (Rosa, 1888) is recorded in Vietnam for the first time. The species is recognized by having three pairs of spermathecal pores in 5/6/7/8, male pores in xviii, presence of copulatory pouches, no genial markings, and manicate intestinal caeca. In addition, three species have been rechecked and re-assigned to different genera, namely Amynthas tripidoporophoratus (Thai & Nguyen, 1993) comb. nov., Metaphire dranfocana (Do & Huynh, 1993) comb. nov., Metaphire anhumalatana (Thai & Huynh, 1993) comb. nov.","PeriodicalId":17683,"journal":{"name":"Jurnal Biodjati","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90294485","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-05-30DOI: 10.15575/biodjati.v5i1.7996
H. Wijayanti, A. Darmawan
Phytoplankton community plays an important ecological role in the aquatic ecosystems as the primary producers and forms the fundament of the aquatic food chain for supporting the water community. Thus, the phytoplankton community structure is a good indicator of water quality due to its sensitiveness to environmental stresses. Two newly dug ponds in Institut Teknologi Sumatera may give an opportunity to study the early colonizing stages of various freshwater communities including phytoplankton. The study attempted to determine the composition and abundance of phytoplankton. Samples were collected from two ponds (A and C) in the reservoir water of Institut Teknologi Sumatera. The content of Phosphorus (P), Nitrogen (N), and Chlorophyll-a (algae biomass) were determined. Phytoplankton had higher diversity in Pond C than Pond A in the study period, in which a total of seven taxa were found, namely Bacillariophycea, Cyanophyceae, Chlorophyceae, Conjugatophyceae, Dinophyceae, Euglenophyceae, Gymnodiniaceae. The most species abundance of both ponds was Peridinium sp. and Trachelomonas sp. The Pond C had the highest mean value of the Shannon-Wiener diversity index. The Linear mixed-effect model showed that low turbidity will result in high phytoplankton diversity. The finding of this study suggests that higher phytoplankton diversity would achieve a natural carrying capacity, and thus would serves as an indicator of ecosystem health.
{"title":"Comparative Study on Phytoplankton Community in Two Newly Dug Ponds in Institut Teknologi Sumatera","authors":"H. Wijayanti, A. Darmawan","doi":"10.15575/biodjati.v5i1.7996","DOIUrl":"https://doi.org/10.15575/biodjati.v5i1.7996","url":null,"abstract":"Phytoplankton community plays an important ecological role in the aquatic ecosystems as the primary producers and forms the fundament of the aquatic food chain for supporting the water community. Thus, the phytoplankton community structure is a good indicator of water quality due to its sensitiveness to environmental stresses. Two newly dug ponds in Institut Teknologi Sumatera may give an opportunity to study the early colonizing stages of various freshwater communities including phytoplankton. The study attempted to determine the composition and abundance of phytoplankton. Samples were collected from two ponds (A and C) in the reservoir water of Institut Teknologi Sumatera. The content of Phosphorus (P), Nitrogen (N), and Chlorophyll-a (algae biomass) were determined. Phytoplankton had higher diversity in Pond C than Pond A in the study period, in which a total of seven taxa were found, namely Bacillariophycea, Cyanophyceae, Chlorophyceae, Conjugatophyceae, Dinophyceae, Euglenophyceae, Gymnodiniaceae. The most species abundance of both ponds was Peridinium sp. and Trachelomonas sp. The Pond C had the highest mean value of the Shannon-Wiener diversity index. The Linear mixed-effect model showed that low turbidity will result in high phytoplankton diversity. The finding of this study suggests that higher phytoplankton diversity would achieve a natural carrying capacity, and thus would serves as an indicator of ecosystem health.","PeriodicalId":17683,"journal":{"name":"Jurnal Biodjati","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84758691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-05-30DOI: 10.15575/biodjati.v5i1.7502
A. S. D. Irsyam, Muhammad Rifqi Hariri, R. R. Irwanto, Wendy A. Mustaqim
According to the Flora of Java, there is only one species of Evolvulus in Java, namely Evolvulus alsinoides. Since then, a second species was reported in 2017 from Universitas Indonesia, Depok, West Java, as an alien naturalized species. Some recent field studies have been conducted to provide updated taxonomic information of the genus on this island. The field observations were carried out in Banten, Jakarta, West Java, Jogjakarta, and East Java from March to December 2019. The herbarium studies were also conducted in Herbarium Bogoriense, Herbarium of Bogor Botanic Gardens, Herbarium Bandungense, and Herbarium of the Department of Biology, Universitas Indonesia. Our study reveals one newly recorded species of Evolvulus in Java already grown in cultivation, namely E. glomeratus Nees & Mart. subsp. grandiflorus (Parodi) Ooststr. Besides that, Evolvulus nummularius is now also reported to occur in other sites of West Java and extended to Central Java. An updated key to Evolvulus in Java, descriptions to both species, photographs, and brief discussions are provided.
根据《爪哇植物志》，爪哇只有一种Evolvulus alsinoides。从那以后，2017年在西爪哇德波的印度尼西亚大学报告了第二个物种，作为外来归化物种。最近进行了一些野外研究，以提供岛上该属的最新分类信息。实地观测于2019年3月至12月在万丹、雅加达、西爪哇、日雅加达和东爪哇进行。植物标本室的研究也在波哥大植物标本室、茂物植物园植物标本室、班敦根斯植物标本室和印度尼西亚大学生物系植物标本室进行。我们的研究揭示了爪哇地区新记录的一种已被人工栽培的Evolvulus，即E. glomeratus Nees & Mart。无性系种群。桔梗(桔梗属)此外，据报道，在西爪哇的其他地点也发现了nummularius Evolvulus，并向中爪哇扩展。提供了爪哇Evolvulus的更新关键，对两个物种的描述，照片和简短的讨论。
{"title":"A Note on The Genus Evolvulus (Convolvulaceae) In Java, Indonesia","authors":"A. S. D. Irsyam, Muhammad Rifqi Hariri, R. R. Irwanto, Wendy A. Mustaqim","doi":"10.15575/biodjati.v5i1.7502","DOIUrl":"https://doi.org/10.15575/biodjati.v5i1.7502","url":null,"abstract":"According to the Flora of Java, there is only one species of Evolvulus in Java, namely Evolvulus alsinoides. Since then, a second species was reported in 2017 from Universitas Indonesia, Depok, West Java, as an alien naturalized species. Some recent field studies have been conducted to provide updated taxonomic information of the genus on this island. The field observations were carried out in Banten, Jakarta, West Java, Jogjakarta, and East Java from March to December 2019. The herbarium studies were also conducted in Herbarium Bogoriense, Herbarium of Bogor Botanic Gardens, Herbarium Bandungense, and Herbarium of the Department of Biology, Universitas Indonesia. Our study reveals one newly recorded species of Evolvulus in Java already grown in cultivation, namely E. glomeratus Nees & Mart. subsp. grandiflorus (Parodi) Ooststr. Besides that, Evolvulus nummularius is now also reported to occur in other sites of West Java and extended to Central Java. An updated key to Evolvulus in Java, descriptions to both species, photographs, and brief discussions are provided.","PeriodicalId":17683,"journal":{"name":"Jurnal Biodjati","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83440797","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-05-30DOI: 10.15575/biodjati.v5i1.5759
Silma Safira Ihya, A. Nuryanto, R. E. Prabowo, D. Bhagawati, Kusbiyanto Kusbiyanto
Marine ornamental trade has been developing for several years with Indonesia as one of the major exporter countries. Among the popular marine ornamental fish in the local and international markets belong to the family of Chaetodontidae. The existence of this family has been reported from several areas in Indonesia. However, no information is available in terms of their phylogenetic relationships of the fish from the south coast of West Java. The aim of this research was to asses the phylogenetic relationships among ornamental fish members of Chaetodontidae in the South Coast of West Java. Fish samples were collected from two trading sites Ujung Genteng and Taman Manalusu. Collected fish were identified using a previously published study. The evolutionary relationships among species were analyzed statistically through phylogenetic analysis based on maximum parsimony algorithm and Kimura 2- Parameter substitution model as implemented in PAUP 4.0 software. Tree branching pattern supported by 1000 bootstraps pseudo-replicates and out-group comparison. Daschyllus trimaculatus used as outgroup species. Based on the study, Chaetodontidae from the South Coast of West Java formed one monophyletic group compared to Dascylus trimaculatus with Chelmon rostratus was the basal species. All the remaining species were the derived species. There are CI and RI value gained. The consistency index (CI) value of this family is 0.5833, and the retention index (RI) is 0.5082.
{"title":"Phylogenetic Relationships of Ornamental Chaetodontidae in The South Coastal of West Java, Indonesia","authors":"Silma Safira Ihya, A. Nuryanto, R. E. Prabowo, D. Bhagawati, Kusbiyanto Kusbiyanto","doi":"10.15575/biodjati.v5i1.5759","DOIUrl":"https://doi.org/10.15575/biodjati.v5i1.5759","url":null,"abstract":"Marine ornamental trade has been developing for several years with Indonesia as one of the major exporter countries. Among the popular marine ornamental fish in the local and international markets belong to the family of Chaetodontidae. The existence of this family has been reported from several areas in Indonesia. However, no information is available in terms of their phylogenetic relationships of the fish from the south coast of West Java. The aim of this research was to asses the phylogenetic relationships among ornamental fish members of Chaetodontidae in the South Coast of West Java. Fish samples were collected from two trading sites Ujung Genteng and Taman Manalusu. Collected fish were identified using a previously published study. The evolutionary relationships among species were analyzed statistically through phylogenetic analysis based on maximum parsimony algorithm and Kimura 2- Parameter substitution model as implemented in PAUP 4.0 software. Tree branching pattern supported by 1000 bootstraps pseudo-replicates and out-group comparison. Daschyllus trimaculatus used as outgroup species. Based on the study, Chaetodontidae from the South Coast of West Java formed one monophyletic group compared to Dascylus trimaculatus with Chelmon rostratus was the basal species. All the remaining species were the derived species. There are CI and RI value gained. The consistency index (CI) value of this family is 0.5833, and the retention index (RI) is 0.5082. ","PeriodicalId":17683,"journal":{"name":"Jurnal Biodjati","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80553078","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-05-30DOI: 10.15575/biodjati.v5i1.7990
Y. G. D. Anggraheni, E. Mulyaningsih, D. Priadi, P. Deswina, Y. Sulistyowati, E. Adi, A. Y. Perdani, F. Nuro, Y. B. Paradisa
SSR marker is one of the genetic markers widely applied in plant breeding programs. The application of molecular markers in plant breeding is meant to accelerate the selection of cross-progeny. The research aimed to identify the SSR primers polymorphism between the parent and control that linked to Al tolerance and verify the cross-progeny of five crosses. The result gained from 37 SSR primers used in this study showed that only nine primers are polymorphic. These nine polymorphic primers are RM257, RM214, RM247, RM205, RM490, RM262, RM569, RM271, and RM19. The application of polymorphic markers on five cross-progeny which have shown the same band pattern as the parents and tolerant control on the use of 9 SSR primers recorded as follows: RM257 2 lines, RM214 5 lines, RM247 5 lines, RM205 lines, RM490 13 lines, RM262 5 lines, RM569 7 lines, RM271 4 lines, and RM19 6 lines. The selected SSR primers linked to Al tolerance in this research can be used as a reference for molecular breeding strategies to develop new Al tolerance rice varieties in dryland conditions.
{"title":"Polymorphic Identification of Simple Sequence Repeat (SSR) Marker for Developing Aluminum-Tolerance Upland Rice","authors":"Y. G. D. Anggraheni, E. Mulyaningsih, D. Priadi, P. Deswina, Y. Sulistyowati, E. Adi, A. Y. Perdani, F. Nuro, Y. B. Paradisa","doi":"10.15575/biodjati.v5i1.7990","DOIUrl":"https://doi.org/10.15575/biodjati.v5i1.7990","url":null,"abstract":"SSR marker is one of the genetic markers widely applied in plant breeding programs. The application of molecular markers in plant breeding is meant to accelerate the selection of cross-progeny. The research aimed to identify the SSR primers polymorphism between the parent and control that linked to Al tolerance and verify the cross-progeny of five crosses. The result gained from 37 SSR primers used in this study showed that only nine primers are polymorphic. These nine polymorphic primers are RM257, RM214, RM247, RM205, RM490, RM262, RM569, RM271, and RM19. The application of polymorphic markers on five cross-progeny which have shown the same band pattern as the parents and tolerant control on the use of 9 SSR primers recorded as follows: RM257 2 lines, RM214 5 lines, RM247 5 lines, RM205 lines, RM490 13 lines, RM262 5 lines, RM569 7 lines, RM271 4 lines, and RM19 6 lines. The selected SSR primers linked to Al tolerance in this research can be used as a reference for molecular breeding strategies to develop new Al tolerance rice varieties in dryland conditions.","PeriodicalId":17683,"journal":{"name":"Jurnal Biodjati","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74739420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-05-30DOI: 10.15575/biodjati.v5i1.6530
U. Susilo, F. Rachmawati
Barred loach is wild fish in which there is no information related to its enzymatic digestive capacity yet. Therefore research on this subject needs to be conducted. The spectrophotometry method was used to study protease, lipase, and amylase activities in barred loach. The study used 89 fish with an average weight of 3.61 ± 0.26g, and 1.68 ± 0.21g. The results showed that pH 7.0 was the highest protease activity. Similarly, lipase activity found between pH 7.0 and pH 8.1. Still, the amylase activity found to be high at pH 8.1. Protease activity also found to be high in the posterior intestine than in the hepato-pancreas and anterior intestine. Lipase and amylase activities did not found differences between hepato-pancreas, anterior intestine, and posterior intestine. In conclusion, the protease, lipase, and amylase activities of barred loach found along the gastrointestinal tract in both large and small fish, which shows that barred loach can digest protein, fat, and carbohydrates in diets better.
{"title":"Protease, Lipase and Amylase Activities in Barred Loach, Nemacheilus Fasciatus C.V.","authors":"U. Susilo, F. Rachmawati","doi":"10.15575/biodjati.v5i1.6530","DOIUrl":"https://doi.org/10.15575/biodjati.v5i1.6530","url":null,"abstract":"Barred loach is wild fish in which there is no information related to its enzymatic digestive capacity yet. Therefore research on this subject needs to be conducted. The spectrophotometry method was used to study protease, lipase, and amylase activities in barred loach. The study used 89 fish with an average weight of 3.61 ± 0.26g, and 1.68 ± 0.21g. The results showed that pH 7.0 was the highest protease activity. Similarly, lipase activity found between pH 7.0 and pH 8.1. Still, the amylase activity found to be high at pH 8.1. Protease activity also found to be high in the posterior intestine than in the hepato-pancreas and anterior intestine. Lipase and amylase activities did not found differences between hepato-pancreas, anterior intestine, and posterior intestine. In conclusion, the protease, lipase, and amylase activities of barred loach found along the gastrointestinal tract in both large and small fish, which shows that barred loach can digest protein, fat, and carbohydrates in diets better.","PeriodicalId":17683,"journal":{"name":"Jurnal Biodjati","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85339336","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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