Pub Date : 2023-05-30DOI: 10.15575/biodjati.v8i1.20260
Jarulis Jarulis, Apin Saputra, R. H. Wibowo
The rate of illegal poaching of blue-winged leafbirds (Chloropsis cochinchinensis) throughout Indonesia, particularly Bengkulu, is quite high. However, only minimal molecular information is available for this species. We performed mtDNA COI gene sequencing to explore genetic characters (conservative site, variable site, parsimony site, and singleton site) of blue-winged leafbirds. Using Qiagen's DNeasy® Blood and Tissue Kit based on the Spin-Column Protocol, total DNA was isolated, and PCR amplification methods were performed. DNA derived from a PCR reaction was forwarded to PT. First Base Malaysia for sequencing. Using MEGA 10.0 and BIOEDIT, the COI gene nucleotide sequence data were assembled, edited, and analyzed to explore of single nucleotide polymorphism, genetic distance, and phylogeny. The 616 bp COI genes contained 566 conservative sites (C), 50 variation sites (V), 24 information parsimony sites (Pi), and 26 singleton sites (S), as indicated by the results. The greatest nucleotide base composition was cytosine (34.1–34.9%), while the lowest was guanine (15.7–16.2%). The proportion of adenine-thymine nucleotide base pairs was greater than that of guanine-cytosine (50.3%). There were 26 barcode-specific mutation sites, 17 transition substitution mutation sites, and 9 transverse substitution mutation sites. The average genetic distance between C. cochinchinensis individuals was 2.2%, but the average genetic difference between species was 9.0%. All C. cochinchinensis individuals in our sample clustered within the same clade and were distinguished from other species within the same genus. The COI gene sequences of C. cochinchinensis that we acquired are novel and can be utilized for molecular identification of the species.
在印度尼西亚各地，尤其是明古鲁省，非法偷猎蓝翅大叶鸟（Chloropsis cochinchinensis）的比例相当高。然而，关于该物种的分子信息却少之又少。我们进行了 mtDNA COI 基因测序，以探索蓝翅大叶鸟的遗传特征（保守位点、可变位点、解析位点和单子位点）。使用Qiagen公司的DNeasy®血液和组织试剂盒（基于旋转柱协议）分离总DNA，并进行PCR扩增。从 PCR 反应中获得的 DNA 将转交给 PT.First Base Malaysia 公司进行测序。利用 MEGA 10.0 和 BIOEDIT，对 COI 基因核苷酸序列数据进行了组装、编辑和分析，以探索单核苷酸多态性、遗传距离和系统发育。结果显示，616 bp的COI基因包含566个保守位点（C）、50个变异位点（V）、24个信息解析位点（Pi）和26个单体位点（S）。核苷酸碱基组成中，胞嘧啶最多（34.1%-34.9%），鸟嘌呤最少（15.7%-16.2%）。腺嘌呤-胸腺嘧啶核苷酸碱基对的比例高于鸟嘌呤-胞嘧啶（50.3%）。条形码特异性突变位点有 26 个，过渡取代突变位点有 17 个，横向取代突变位点有 9 个。C. cochinchinensis个体间的平均遗传距离为2.2%，但物种间的平均遗传差异为9.0%。我们样本中的所有 C. cochinchinensis 个体都聚在同一支系中，并与同属的其他物种区分开来。我们获得的 C. cochinchinensis COI 基因序列具有新颖性，可用于该物种的分子鉴定。
{"title":"Genetic Characteristics of Chloropsis cochinchinensis Gmelin, 1789 Based on The Mitochondrial DNA COI Gene","authors":"Jarulis Jarulis, Apin Saputra, R. H. Wibowo","doi":"10.15575/biodjati.v8i1.20260","DOIUrl":"https://doi.org/10.15575/biodjati.v8i1.20260","url":null,"abstract":"The rate of illegal poaching of blue-winged leafbirds (Chloropsis cochinchinensis) throughout Indonesia, particularly Bengkulu, is quite high. However, only minimal molecular information is available for this species. We performed mtDNA COI gene sequencing to explore genetic characters (conservative site, variable site, parsimony site, and singleton site) of blue-winged leafbirds. Using Qiagen's DNeasy® Blood and Tissue Kit based on the Spin-Column Protocol, total DNA was isolated, and PCR amplification methods were performed. DNA derived from a PCR reaction was forwarded to PT. First Base Malaysia for sequencing. Using MEGA 10.0 and BIOEDIT, the COI gene nucleotide sequence data were assembled, edited, and analyzed to explore of single nucleotide polymorphism, genetic distance, and phylogeny. The 616 bp COI genes contained 566 conservative sites (C), 50 variation sites (V), 24 information parsimony sites (Pi), and 26 singleton sites (S), as indicated by the results. The greatest nucleotide base composition was cytosine (34.1–34.9%), while the lowest was guanine (15.7–16.2%). The proportion of adenine-thymine nucleotide base pairs was greater than that of guanine-cytosine (50.3%). There were 26 barcode-specific mutation sites, 17 transition substitution mutation sites, and 9 transverse substitution mutation sites. The average genetic distance between C. cochinchinensis individuals was 2.2%, but the average genetic difference between species was 9.0%. All C. cochinchinensis individuals in our sample clustered within the same clade and were distinguished from other species within the same genus. The COI gene sequences of C. cochinchinensis that we acquired are novel and can be utilized for molecular identification of the species.","PeriodicalId":17683,"journal":{"name":"Jurnal Biodjati","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139372451","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-30DOI: 10.15575/biodjati.v8i1.25132
Anwar Rovik, Clearensia Ade Bina Noviani
Scientists are performing various measurements to overcome the COVID-19 pandemic. The genomic mutations of SARS-CoV-2 can change their pathogenicity, infectivity, transmission, and antigenicity. This present study aimed to know a) the genetic variation of structural and functional genes of SARS-CoV-2 circulating in Banyumas and b) the potential of the Cilacap’s Harbour as a human mobility portal on the genetic variations of SARS-CoV-2 circulating in Banyumas, Central Java (Indonesia). Genomic sequence of SARS-CoV-2 isolates were taken from the Global Initiative on Sharing All Influenza Data (GISAID) and the National Center for Biotechnology Information (NCBI) online platforms. A gene cut was carried out from Wuhan reference isolate, fifteen isolates from Banyumas, and two isolates from Cilacap (Central Java) using Unipro UGENE v. 33.0 software, considering the annotation of the Wuhan-1 isolate. Genetic variations were detected among SARS-CoV-2 isolates circulating in Banyumas. The structural protein (envelope, membrane, nucleocapsid) encoding gene and the RdRp gene were highly conserved to Wuhan reference genome (Wuhan-Hu-1). Meanwhile, the ORF and the spike-encoding genes were less identical to the Wuhan reference genome. This study also proposed that human mobility from outside Central Java through Cilacap’s Harbour did not affect the genetic variation of SARS-CoV-2 isolates circulating in Banyumas (Central Java).
{"title":"Genetic Variation of Structural and Functional Genes of SARS-CoV-2 Isolates Circulating in Banyumas (Indonesia)","authors":"Anwar Rovik, Clearensia Ade Bina Noviani","doi":"10.15575/biodjati.v8i1.25132","DOIUrl":"https://doi.org/10.15575/biodjati.v8i1.25132","url":null,"abstract":"Scientists are performing various measurements to overcome the COVID-19 pandemic. The genomic mutations of SARS-CoV-2 can change their pathogenicity, infectivity, transmission, and antigenicity. This present study aimed to know a) the genetic variation of structural and functional genes of SARS-CoV-2 circulating in Banyumas and b) the potential of the Cilacap’s Harbour as a human mobility portal on the genetic variations of SARS-CoV-2 circulating in Banyumas, Central Java (Indonesia). Genomic sequence of SARS-CoV-2 isolates were taken from the Global Initiative on Sharing All Influenza Data (GISAID) and the National Center for Biotechnology Information (NCBI) online platforms. A gene cut was carried out from Wuhan reference isolate, fifteen isolates from Banyumas, and two isolates from Cilacap (Central Java) using Unipro UGENE v. 33.0 software, considering the annotation of the Wuhan-1 isolate. Genetic variations were detected among SARS-CoV-2 isolates circulating in Banyumas. The structural protein (envelope, membrane, nucleocapsid) encoding gene and the RdRp gene were highly conserved to Wuhan reference genome (Wuhan-Hu-1). Meanwhile, the ORF and the spike-encoding genes were less identical to the Wuhan reference genome. This study also proposed that human mobility from outside Central Java through Cilacap’s Harbour did not affect the genetic variation of SARS-CoV-2 isolates circulating in Banyumas (Central Java).","PeriodicalId":17683,"journal":{"name":"Jurnal Biodjati","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139372505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-30DOI: 10.15575/biodjati.v8i1.20190
I. Q. Lailaty, Muhammad Efendi
Anatomical studies have been used in the classification and identification of Begonia species. However, the research on Indonesian native Begonia is still limited. This study focused to evaluate the importance of leaves’ anatomy character for the identification and classification purpose of the Sphenanthera group in Java and Sumatra. A total of six individuals of four Begonia species used in this study, namely Begonia multangula, B. longifolia, B. pseudoscottii, and B. robusta. This study aimed to describe the anatomical characters of the leaves that are useful in the identification and grouping of Begonia section Platycentrum–Sphenanthera. The anatomical preparations used the paraffin method for the cross-section of leaves and the semi-permanent method for paradermal observations. In general, the epidermal cells of paradermal Begonia leaves are polygonal in shape. Consistently, Begonias have single-type stomata with an anisocytic type which are only present on the abaxial leaves. Begonia Sphenanthera group has a thin cuticle (less than 2 µm), both in the abaxial and adaxial surfaces. The lower epidermis cells are smaller than the upper epidermis cells. The mesophyll is differentiated into palisade and spongy tissue. The number and form of mesophyll tissue are varied, therefore useful for diagnosing an important character in the grouping of Begonias from this section.
{"title":"Leaf Anatomy Profile of Begonia Section Platycentrum Sphenanthera Group From Java and Sumatera, Indonesia","authors":"I. Q. Lailaty, Muhammad Efendi","doi":"10.15575/biodjati.v8i1.20190","DOIUrl":"https://doi.org/10.15575/biodjati.v8i1.20190","url":null,"abstract":"Anatomical studies have been used in the classification and identification of Begonia species. However, the research on Indonesian native Begonia is still limited. This study focused to evaluate the importance of leaves’ anatomy character for the identification and classification purpose of the Sphenanthera group in Java and Sumatra. A total of six individuals of four Begonia species used in this study, namely Begonia multangula, B. longifolia, B. pseudoscottii, and B. robusta. This study aimed to describe the anatomical characters of the leaves that are useful in the identification and grouping of Begonia section Platycentrum–Sphenanthera. The anatomical preparations used the paraffin method for the cross-section of leaves and the semi-permanent method for paradermal observations. In general, the epidermal cells of paradermal Begonia leaves are polygonal in shape. Consistently, Begonias have single-type stomata with an anisocytic type which are only present on the abaxial leaves. Begonia Sphenanthera group has a thin cuticle (less than 2 µm), both in the abaxial and adaxial surfaces. The lower epidermis cells are smaller than the upper epidermis cells. The mesophyll is differentiated into palisade and spongy tissue. The number and form of mesophyll tissue are varied, therefore useful for diagnosing an important character in the grouping of Begonias from this section.","PeriodicalId":17683,"journal":{"name":"Jurnal Biodjati","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139372671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-30DOI: 10.15575/biodjati.v8i1.25029
U. Susilo, Yulia Sistina, Eko Setio Wibowo, Hanan Hassan Alsheikh Mahmoud, Hana Hana, F. Rachmawati
Many studies have been conducted on the feeding frequency in fish, but there is no information on R. lateristriata. Therefore, this study aimed to determine yellow rasbora growth performance, feed efficiency, and digestive enzyme activity at different feeding frequencies. This research was conducted experimentally with four treatments and three replications. The treatments include: P1 fish were fed two times/day; P2 fish were fed three times/day; P3 fish were fed four times/day; and P4 fish were fed five times/day. In this experiment, 420 two-month-old yellow rasboras were used. The experimental results showed that body weight gain, RGR, SGR, CF, FCR, PER, body lipid & protein levels, protein and lipid retention, protease, and amylase activity were not significantly different (p>0.05) between different feeding frequencies. However, lipase and alkaline phosphatase activities differed significantly among the different feeding frequencies. Growth performance, feed efficiency, protease activity, and yellow rasbora amylase are not affected by feeding frequency between two to five times a day. Still, the highest lipase and alkaline phosphatase activity were achieved at the feeding frequency four times a day, but no effect on growth. So, feeding twice a day is considered quite efficient for the culture of yellow rasbora. The results of this study can contribute to the development of fish farming, especially the yellow rasbora, in the future.
{"title":"Growth, Food Efficiency, and Enzyme Activities in Yellow Rasbora (Rasbora lateristriata) at Different Feeding Frequencies","authors":"U. Susilo, Yulia Sistina, Eko Setio Wibowo, Hanan Hassan Alsheikh Mahmoud, Hana Hana, F. Rachmawati","doi":"10.15575/biodjati.v8i1.25029","DOIUrl":"https://doi.org/10.15575/biodjati.v8i1.25029","url":null,"abstract":"Many studies have been conducted on the feeding frequency in fish, but there is no information on R. lateristriata. Therefore, this study aimed to determine yellow rasbora growth performance, feed efficiency, and digestive enzyme activity at different feeding frequencies. This research was conducted experimentally with four treatments and three replications. The treatments include: P1 fish were fed two times/day; P2 fish were fed three times/day; P3 fish were fed four times/day; and P4 fish were fed five times/day. In this experiment, 420 two-month-old yellow rasboras were used. The experimental results showed that body weight gain, RGR, SGR, CF, FCR, PER, body lipid & protein levels, protein and lipid retention, protease, and amylase activity were not significantly different (p>0.05) between different feeding frequencies. However, lipase and alkaline phosphatase activities differed significantly among the different feeding frequencies. Growth performance, feed efficiency, protease activity, and yellow rasbora amylase are not affected by feeding frequency between two to five times a day. Still, the highest lipase and alkaline phosphatase activity were achieved at the feeding frequency four times a day, but no effect on growth. So, feeding twice a day is considered quite efficient for the culture of yellow rasbora. The results of this study can contribute to the development of fish farming, especially the yellow rasbora, in the future.","PeriodicalId":17683,"journal":{"name":"Jurnal Biodjati","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139372683","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-30DOI: 10.15575/biodjati.v8i1.19968
Retno Susilowati, Ulfa Maynisa Rohmanningrum
High levels of free radicals in diabetic wounds often cause chronic inflammation. Nigella sativa and Trigonella foenum-graecum seed extracts are rich in antioxidants and have anti-inflammatory effects. Trigonella foenum-graecum seed extracts can also accelerate the reepithelialization process in wound healing. This study aimed to determine the combination of ethanol extract of Nigella sativa and Trigonella foenum-graecum seeds on wound length and the number of neutrophils, macrophages, fibroblasts, and collagen density in the incision wound of diabetic mice induced by streptozotocin. T. This study used three types of controls, namely Normal (Normal mice, HPMC 3%), DM (Diabetic mice, HPMC 3%), and Iodine (Diabetic mice, Povidone Iodine 10%). Topical treatment with 70% ethanol extract ointment combined with Nigella sativa and Trigonella foenum-graecum in various variations, namely N (20%:0%), T (0%:10%), NT (10%:5%), Observations of the wound healing process were carried out on 3rd, 7th and 14th day. Data were analyzed using the One Way ANOVA and Duncan Multiple Range Test. The study's results by observing the morphology of the wound length on day 7th day showed that the combination treatment of extracts of Nigella sativa and Trigonella foenum-graecum resulted in the shortest wound, which was significantly different from the wound in untreated diabetic mice. In addition, diabetic wounds without treatment showed infection, and on the contrary, the infection did not occur in wounds treated with a combination of extracts of Nigella sativa and Trigonella foenum-graecum. The results of wound histology observations showed that the combination treatment of extracts of Nigella sativa and Trigonella foenum-graecum showed a decrease in inflammation which was indicated by a significant decrease in the number of neutrophil cells, macrophages, and accelerated reepithelialization of wound healing as indicated by a significant increase in the number of fibroblasts and collagen density since the third day of treatment.
{"title":"Effective Combination of Nigella sativa and Trigonella foenum-graecum Seed Extract on Wound Healing in Diabetic Mice","authors":"Retno Susilowati, Ulfa Maynisa Rohmanningrum","doi":"10.15575/biodjati.v8i1.19968","DOIUrl":"https://doi.org/10.15575/biodjati.v8i1.19968","url":null,"abstract":"High levels of free radicals in diabetic wounds often cause chronic inflammation. Nigella sativa and Trigonella foenum-graecum seed extracts are rich in antioxidants and have anti-inflammatory effects. Trigonella foenum-graecum seed extracts can also accelerate the reepithelialization process in wound healing. This study aimed to determine the combination of ethanol extract of Nigella sativa and Trigonella foenum-graecum seeds on wound length and the number of neutrophils, macrophages, fibroblasts, and collagen density in the incision wound of diabetic mice induced by streptozotocin. T. This study used three types of controls, namely Normal (Normal mice, HPMC 3%), DM (Diabetic mice, HPMC 3%), and Iodine (Diabetic mice, Povidone Iodine 10%). Topical treatment with 70% ethanol extract ointment combined with Nigella sativa and Trigonella foenum-graecum in various variations, namely N (20%:0%), T (0%:10%), NT (10%:5%), Observations of the wound healing process were carried out on 3rd, 7th and 14th day. Data were analyzed using the One Way ANOVA and Duncan Multiple Range Test. The study's results by observing the morphology of the wound length on day 7th day showed that the combination treatment of extracts of Nigella sativa and Trigonella foenum-graecum resulted in the shortest wound, which was significantly different from the wound in untreated diabetic mice. In addition, diabetic wounds without treatment showed infection, and on the contrary, the infection did not occur in wounds treated with a combination of extracts of Nigella sativa and Trigonella foenum-graecum. The results of wound histology observations showed that the combination treatment of extracts of Nigella sativa and Trigonella foenum-graecum showed a decrease in inflammation which was indicated by a significant decrease in the number of neutrophil cells, macrophages, and accelerated reepithelialization of wound healing as indicated by a significant increase in the number of fibroblasts and collagen density since the third day of treatment.","PeriodicalId":17683,"journal":{"name":"Jurnal Biodjati","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139372544","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-11-30DOI: 10.15575/biodjati.v7i2.20279
Kahar Muzakhar, Farah Salma Elida, Ramdhan Putrasetya, S. Siswoyo, Rudju Winarsa, H. T. Wiyono
An α-1,4-glycosidic bonds galactoses pectin, mainly composed of a D-galacturonic acid chain, are important biomaterial widely used in industries. Utilizing this material, a bioprocess, including the biocatalysis pectinase, is often needed. Pectinase production was optimized in 7 days SSF at 37°C, and the pectinase activities were daily measured by the method of Somogy-Nelson. The optimum pectinase production was 0.166 U/ml on the fourth day SSF. Purification using open column ion exchange chromatography DEAE cellulose DE-52 resulted in 1030.9 folds of pectinase purity with a yield of 25.9%. The enzyme was at optimal activity at pH six and attended stable in the pH range of 5.5-8, while optimal activity at a temperature of 50°C and was stable in the range of 30-45°C. The pectinase activity increased by 120% with the addition of 10 mM Mg2+, and 95% retained when 10 mM Ca2+ was added. The presence of 10 mM Na+, K+, and Fe2+ resulted in a slight effect of activity at 85%, 83%, and 78%. However, it was strongly inhibited by 10 mM Al3+ and retained 25%. Based on the results above, the microbial utilization of coffee pulp waste by ISH16 bacteria pectinolytic is one opportunity to produce valuable pectinase with low-cost production, so comprehensive examination in large-scale production is needed too. In this paper, all research detail steps were described.
α-1,4-糖苷键半乳糖果胶，主要由d -半乳糖醛酸链组成，是工业上广泛应用的重要生物材料。利用这种材料，通常需要一个生物过程，包括生物催化果胶酶。37℃条件下发酵7 d，优化果胶酶产量，并采用Somogy-Nelson法每日测定果胶酶活性。发酵第4天，最佳果胶酶产量为0.166 U/ml。采用开柱离子交换色谱法纯化DEAE纤维素DE-52，其果胶酶纯度为1030.9倍，产率为25.9%。该酶在pH值为6时活性最佳，在pH值为5.5 ~ 8时稳定;在温度为50℃时活性最佳，在30 ~ 45℃范围内稳定。添加10 mM Mg2+时，果胶酶活性提高了120%，添加10 mM Ca2+时，果胶酶活性保持了95%。10 mM Na+、K+和Fe2+的存在对活性的影响较小，分别为85%、83%和78%。10 mM Al3+对其抑制作用较强，保留25%。综上所述，利用ISH16菌对咖啡废渣进行果胶降解是低成本生产有价值的果胶酶的一个机会，还需要在规模化生产中进行综合考察。本文对研究的各个细节步骤进行了描述。
{"title":"An Extracellular Pectinase from ISH16 Bacteria Isolated Induced by Coffee Pulp Waste Substrate","authors":"Kahar Muzakhar, Farah Salma Elida, Ramdhan Putrasetya, S. Siswoyo, Rudju Winarsa, H. T. Wiyono","doi":"10.15575/biodjati.v7i2.20279","DOIUrl":"https://doi.org/10.15575/biodjati.v7i2.20279","url":null,"abstract":"An α-1,4-glycosidic bonds galactoses pectin, mainly composed of a D-galacturonic acid chain, are important biomaterial widely used in industries. Utilizing this material, a bioprocess, including the biocatalysis pectinase, is often needed. Pectinase production was optimized in 7 days SSF at 37°C, and the pectinase activities were daily measured by the method of Somogy-Nelson. The optimum pectinase production was 0.166 U/ml on the fourth day SSF. Purification using open column ion exchange chromatography DEAE cellulose DE-52 resulted in 1030.9 folds of pectinase purity with a yield of 25.9%. The enzyme was at optimal activity at pH six and attended stable in the pH range of 5.5-8, while optimal activity at a temperature of 50°C and was stable in the range of 30-45°C. The pectinase activity increased by 120% with the addition of 10 mM Mg2+, and 95% retained when 10 mM Ca2+ was added. The presence of 10 mM Na+, K+, and Fe2+ resulted in a slight effect of activity at 85%, 83%, and 78%. However, it was strongly inhibited by 10 mM Al3+ and retained 25%. Based on the results above, the microbial utilization of coffee pulp waste by ISH16 bacteria pectinolytic is one opportunity to produce valuable pectinase with low-cost production, so comprehensive examination in large-scale production is needed too. In this paper, all research detail steps were described.","PeriodicalId":17683,"journal":{"name":"Jurnal Biodjati","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84389389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-11-30DOI: 10.15575/biodjati.v7i2.18616
D. Lestari, Fatimatuzzahra Fatimatuzzahra, Agnes Petra Sianipar, Shahnaz Shabrina Wulansari
Allopurinol is used to reduce total uric acid levels in the body into oxypurinol which can inhibit xanthine oxidase. Allopurinol inhibits the precursors of uric acid formation, xanthine, and hypoxanthine. However, consumption of the drugs can cause side effects on the liver. The aim of the research was to determine the effect of allopurinol induction on the liver histopathology of male mice (Mus musculus) DDY strain. The method used in this research was an experimental design used post-test only that was divided into 4 groups of 4 mice per group. The control group (P0) was given 0.5% Na-CMC, and groups I, II, and III (P1, P2, and P3) were induced by allopurinol at 10 mg/kg BW, 20 mg/kg BW, and 30 mg/kg BW for 14 days. Allopurinol induction was performed by oral gavage. The results of the research showed that treatment with allopurinol caused changes in the mice’s body weight, liver index, liver morphology, and histological structure of the liver tissue, including necrosis, steatosis, leukocyte infiltration, binuclear hepatocytes, hepatocyte swelling, congestion, sinusoid dilatation, and hemorrhage. The level of liver damage increased in line with the dose used. This research indicated that the higher the allopurinol level, the higher the level of alteration in the liver section structure. Long-term use of allopurinol can cause damage to the structure of mice liver (liver toxicity).
{"title":"Allopurinol Induction on Histopathological Structure of the Liver in Male Mice (Mus musculus)","authors":"D. Lestari, Fatimatuzzahra Fatimatuzzahra, Agnes Petra Sianipar, Shahnaz Shabrina Wulansari","doi":"10.15575/biodjati.v7i2.18616","DOIUrl":"https://doi.org/10.15575/biodjati.v7i2.18616","url":null,"abstract":"Allopurinol is used to reduce total uric acid levels in the body into oxypurinol which can inhibit xanthine oxidase. Allopurinol inhibits the precursors of uric acid formation, xanthine, and hypoxanthine. However, consumption of the drugs can cause side effects on the liver. The aim of the research was to determine the effect of allopurinol induction on the liver histopathology of male mice (Mus musculus) DDY strain. The method used in this research was an experimental design used post-test only that was divided into 4 groups of 4 mice per group. The control group (P0) was given 0.5% Na-CMC, and groups I, II, and III (P1, P2, and P3) were induced by allopurinol at 10 mg/kg BW, 20 mg/kg BW, and 30 mg/kg BW for 14 days. Allopurinol induction was performed by oral gavage. The results of the research showed that treatment with allopurinol caused changes in the mice’s body weight, liver index, liver morphology, and histological structure of the liver tissue, including necrosis, steatosis, leukocyte infiltration, binuclear hepatocytes, hepatocyte swelling, congestion, sinusoid dilatation, and hemorrhage. The level of liver damage increased in line with the dose used. This research indicated that the higher the allopurinol level, the higher the level of alteration in the liver section structure. Long-term use of allopurinol can cause damage to the structure of mice liver (liver toxicity). ","PeriodicalId":17683,"journal":{"name":"Jurnal Biodjati","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82666498","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-11-30DOI: 10.15575/biodjati.v7i2.18524
A. P. Nugroho, Dwi Puji Hayati
The soil in the Landfill area has generally been contaminated by various types of pollutants, including heavy metals, microplastics, ammonia, chloride, benzene, toluene, ethylene, ethylene benzene, and xylene (BTEX). At high concentrations, the pollutant can cause toxic effects on plants. The study aimed to evaluate the morphological and physiological conditions of C. argentea L. and C. rutidosperma D.C. after being planted on Randukuning landfill soil. Plant species were planted on landfill soil in the greenhouse for two months. Morphological observations (stem length, root length, and the number of leaves) and physiological (biomass of roots, stems, and leaves) were carried out on days 0, 3, 7, 14, 21, and 28 after planting on landfill soil. The results showed that C. argentea L. and C. rutidosperma D.C. did not show morphological and physiological effects. Both species can grow well on landfill soils. Therefore, these species can be potential phytoremediation agents.
填埋区土壤普遍受到重金属、微塑料、氨、氯化物、苯、甲苯、乙烯、乙苯和二甲苯(BTEX)等多种污染物的污染。高浓度时，这种污染物会对植物产生毒性作用。本研究旨在评价在兰杜库宁垃圾填埋场土壤上种植后的银青菇(C. argentea L.)和松果菇(C. rutidosperma dc .)的形态和生理状况。在温室的填埋土壤上种植各种植物两个月。分别于种植后第0、3、7、14、21和28天进行形态观察(茎长、根长和叶数)和生理观察(根、茎和叶生物量)。结果表明，银杏叶和芦桃叶不表现出形态和生理效应。这两种植物都能在垃圾填埋场的土壤上生长良好。因此，这些物种可能是潜在的植物修复剂。
{"title":"Soil Contamination in Randukuning Landfill: Morphological-Physiological Responses of Celosia argentea L. and Cleome rutidosperma D.C.","authors":"A. P. Nugroho, Dwi Puji Hayati","doi":"10.15575/biodjati.v7i2.18524","DOIUrl":"https://doi.org/10.15575/biodjati.v7i2.18524","url":null,"abstract":"The soil in the Landfill area has generally been contaminated by various types of pollutants, including heavy metals, microplastics, ammonia, chloride, benzene, toluene, ethylene, ethylene benzene, and xylene (BTEX). At high concentrations, the pollutant can cause toxic effects on plants. The study aimed to evaluate the morphological and physiological conditions of C. argentea L. and C. rutidosperma D.C. after being planted on Randukuning landfill soil. Plant species were planted on landfill soil in the greenhouse for two months. Morphological observations (stem length, root length, and the number of leaves) and physiological (biomass of roots, stems, and leaves) were carried out on days 0, 3, 7, 14, 21, and 28 after planting on landfill soil. The results showed that C. argentea L. and C. rutidosperma D.C. did not show morphological and physiological effects. Both species can grow well on landfill soils. Therefore, these species can be potential phytoremediation agents.","PeriodicalId":17683,"journal":{"name":"Jurnal Biodjati","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86983945","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-11-30DOI: 10.15575/biodjati.v7i2.20439
B. Prahardika, Widi Muhammad Lutsfi Dwi Styawan
Diatoms are widely used as bioindicators of water quality because of their short life cycle and sensitivity to changes of environmental conditions in waters. One type of diatom that is used as a bioindicator is an epilithic diatom. Epilithic diatoms are one of the diatom microalgae communities attached to rock, so they are difficult to be carried away by currents. This study aimed to determine the type, composition, abundance, diversity, and dominance of epilithic diatoms found in the waters of the Brangkal subwatershed, and to determine the quality of water in the Brangkal subwatershed based on the epilithic diatom tropic index. Epilithic diatom samples were taken from 4 stations in the Brangkal subwatershed, Mojokerto Regency at the beginning of the dry season (July-August). The analysis carried out includes abundance, diversity index, dominance index, and Diatom Trophic Index (TDI). Based on the results of the study, 17 genera of epilithic diatoms were found, with the highest abundance of epilithic diatoms, including Navicula, Cocconeis, Nitszchia, and Gomphonema. The diversity index of all stations was in the medium category, with a value between 1.206 to 1.882. The lowest dominance index at station 1 was 0.167 and the highest was at station 4 with 0.373. In addition, the observed water quality is based on the Diatom Trophic Index (TDI), stations 1 to 3 are in a good category, and stations 4 are in a bad category.
{"title":"The Epilithic Diatom Community as a Bioindicator of water quality Brangkal Subwatershed in the Mojokerto Area","authors":"B. Prahardika, Widi Muhammad Lutsfi Dwi Styawan","doi":"10.15575/biodjati.v7i2.20439","DOIUrl":"https://doi.org/10.15575/biodjati.v7i2.20439","url":null,"abstract":"Diatoms are widely used as bioindicators of water quality because of their short life cycle and sensitivity to changes of environmental conditions in waters. One type of diatom that is used as a bioindicator is an epilithic diatom. Epilithic diatoms are one of the diatom microalgae communities attached to rock, so they are difficult to be carried away by currents. This study aimed to determine the type, composition, abundance, diversity, and dominance of epilithic diatoms found in the waters of the Brangkal subwatershed, and to determine the quality of water in the Brangkal subwatershed based on the epilithic diatom tropic index. Epilithic diatom samples were taken from 4 stations in the Brangkal subwatershed, Mojokerto Regency at the beginning of the dry season (July-August). The analysis carried out includes abundance, diversity index, dominance index, and Diatom Trophic Index (TDI). Based on the results of the study, 17 genera of epilithic diatoms were found, with the highest abundance of epilithic diatoms, including Navicula, Cocconeis, Nitszchia, and Gomphonema. The diversity index of all stations was in the medium category, with a value between 1.206 to 1.882. The lowest dominance index at station 1 was 0.167 and the highest was at station 4 with 0.373. In addition, the observed water quality is based on the Diatom Trophic Index (TDI), stations 1 to 3 are in a good category, and stations 4 are in a bad category. ","PeriodicalId":17683,"journal":{"name":"Jurnal Biodjati","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74664798","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-11-30DOI: 10.15575/biodjati.v7i2.14298
R. Susilowati, Budi Hartono
Permot (Passiflora foetida) is one type of plant that has been used as a mosquito coil. Besides being effective at killing Aedes aegypti mosquitoes, mosquito coils made from permot leaf extract are also effective at killing German cockroaches and house flies. Until now there have been no reports stating the side effects of using permot leaf extract in humans. This study was designed to demonstrate the harmful side effects of inhalation of smoked mosquito coils made from permot leaf extract on the cerebellar cortex of adult male rats. Thirty-five adult male rats were used in this study. Rats were divided into 7 groups, namely group 1 as a negative control (without exposure), group 2 as a positive control (transfluthrin exposure 3000 ppm), groups 3 to 7 were treatment groups of permot leaf extract with graded doses (500 ppm, 1000 ppm, 2000 ppm, 3000 ppm, and 4000 ppm), and was treated for 12 weeks. All rats were sacrificed in a timely manner and cerebellar specimens were taken, prepared and observed using a light microscope. Previously, rats were weighed periodically to determine the effect of toxicity. The results showed that exposure to mosquito coil smoke made from 3000 ppm Transflutrin and 4000 ppm permot leaf extract caused weight loss in rats. Likewise on the weight of the rat cerebellum. The effect of decreasing the number of Purkinje cells also occurred in the group exposed to mosquito coils made from Transflutrin 3000 ppm and permot leaf extract 4000 ppm. Therefore, the safe and effective dose of mosquito coils made from permot leaves is less than 4000 ppm, because exposure to doses of more than 4000 ppm can cause degenerative changes in the cerebellar cortex.
{"title":"Effects of Passiflora foetida on the Number of Purkinje Cells in the Cerebellum of Albino Rats","authors":"R. Susilowati, Budi Hartono","doi":"10.15575/biodjati.v7i2.14298","DOIUrl":"https://doi.org/10.15575/biodjati.v7i2.14298","url":null,"abstract":" Permot (Passiflora foetida) is one type of plant that has been used as a mosquito coil. Besides being effective at killing Aedes aegypti mosquitoes, mosquito coils made from permot leaf extract are also effective at killing German cockroaches and house flies. Until now there have been no reports stating the side effects of using permot leaf extract in humans. This study was designed to demonstrate the harmful side effects of inhalation of smoked mosquito coils made from permot leaf extract on the cerebellar cortex of adult male rats. Thirty-five adult male rats were used in this study. Rats were divided into 7 groups, namely group 1 as a negative control (without exposure), group 2 as a positive control (transfluthrin exposure 3000 ppm), groups 3 to 7 were treatment groups of permot leaf extract with graded doses (500 ppm, 1000 ppm, 2000 ppm, 3000 ppm, and 4000 ppm), and was treated for 12 weeks. All rats were sacrificed in a timely manner and cerebellar specimens were taken, prepared and observed using a light microscope. Previously, rats were weighed periodically to determine the effect of toxicity. The results showed that exposure to mosquito coil smoke made from 3000 ppm Transflutrin and 4000 ppm permot leaf extract caused weight loss in rats. Likewise on the weight of the rat cerebellum. The effect of decreasing the number of Purkinje cells also occurred in the group exposed to mosquito coils made from Transflutrin 3000 ppm and permot leaf extract 4000 ppm. Therefore, the safe and effective dose of mosquito coils made from permot leaves is less than 4000 ppm, because exposure to doses of more than 4000 ppm can cause degenerative changes in the cerebellar cortex.","PeriodicalId":17683,"journal":{"name":"Jurnal Biodjati","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77777577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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