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Antiparasitic Effects and Cellular Mechanisms of Formononetin (a Natural Isoflavone) Against Hydatid Cyst Protoscoleces Formononetin(一种天然的异黄酮)对棘球蚴的抗寄生虫作用及其细胞机制
IF 0.6 Q4 PHARMACOLOGY & PHARMACY Pub Date : 2023-03-30 DOI: 10.5812/jjnpp-129302
Setareh Dastyar Haghighi, Amal Khudair Khalaf, H. Mahmoudvand, A. Adineh, Ali Mohammad Maleki, Javad Ghasemian Yadegari
Background: The chemical agents applied to reduce the complications of hydatid cyst surgery are not free of side effects. Formononetin (FMN), as a natural isoflavone, has been shown to have various therapeutic benefits. Objectives: We studied the in vitro and ex vivo protoscolicidal activity and cellular mechanisms of FMN against hydatid cyst protoscoleces. Methods: Formononetin at 75, 150, and 300 μg/mL was mixed with hydatid cyst protoscoleces (103/mL), and the viability was determined in 5 to 60 min through eosin staining assay. The effect of FMN on caspase-3 activity was tested through a commercial colorimetric protease kit. In addition, the penetrability of the plasma membrane of protoscoleces after exposure to FMN was also determined by the SYTOX assay. Results: Formononetin at 300 μg/mL completely destroyed hydatid cyst protoscoleces after 30 minutes. While at 150 μg/mL, after 60 minutes of contact, 100% of protoscoleces were eliminated. By ex vivo assay, FMN showed its antiparasitic effect for longer periods. Based on these results, FMN at 300 μg/mL concentration completely eliminated hydatid cyst protoscoleces after 60 minutes of exposure. While at a concentration of 150 μg/mL after 60 minutes, 89.6% of protoscoleces were destroyed. Formononetin noticeably increased (P < 0.001) the activity of caspase-3 and the permeability of protoscoleces dose-dependently. Conclusions: Formononetin as a natural product showed promising effects on the protoscoleces of hydatid cysts, indicating that it can be considered a valuable scolicidal agent. However, additional investigations are necessary to evaluate its efficacy in animal models and human subjects.
背景:用于减少棘球蚴手术并发症的化学制剂并非没有副作用。马齿苋素(FMN)作为一种天然异黄酮,已被证明具有多种治疗作用。目的:研究FMN对棘球蚴原头节的体外和离体原头节活性及细胞机制。方法:将75、150和300μg/mL的Formononetin与103个/mL的棘球蚴混合,用伊红染色法在5~60min内测定其活力。通过商业比色蛋白酶试剂盒测试FMN对胱天蛋白酶-3活性的影响。此外,还通过SYTOX测定法测定了FMN暴露后原头节细胞质膜的穿透性。结果:300μg/mL的甲素在30分钟后完全破坏了棘球蚴的原头节。而在150μg/mL下,接触60分钟后,100%的原头节被消除。离体实验表明,FMN具有较长时间的抗寄生虫作用。基于这些结果,300μg/mL浓度的FMN在暴露60分钟后完全消除了棘球蚴原头节。当浓度为150μg/mL时,60分钟后,89.6%的原头节被破坏。Formononetin显著增加胱天蛋白酶-3的活性和原皮质的通透性(P<0.001)。结论:Formononetin作为一种天然产物,对棘球蚴囊肿的原头节有很好的治疗作用,是一种有价值的治疗棘球蚴病的药物。然而,还需要进一步的研究来评估其在动物模型和人类受试者中的疗效。
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引用次数: 0
Flavonoids in the Treatment of Glioblastoma Using Niosomal Nanocarrier 黄酮类化合物在纳米粒子载体治疗胶质母细胞瘤中的作用
IF 0.6 Q4 PHARMACOLOGY & PHARMACY Pub Date : 2023-02-15 DOI: 10.5812/jjnpp-135027
Mahmoud Gharbavi, A. Mojaddami
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引用次数: 1
Current Status of Peptide Medications and the Position of Active Therapeutic Peptides with Scorpion Venom Origin 多肽药物研究现状及蝎毒源活性治疗肽的地位
IF 0.6 Q4 PHARMACOLOGY & PHARMACY Pub Date : 2023-02-14 DOI: 10.5812/jjnpp-134049
M. Baradaran
: Peptides are highly potent, selective, and relatively safe therapeutics. Over the past two decades, natural peptides have been obtained, studied, and eventually approved by the Food and Drug Administration (FDA) due to advancements in identification, production, modification, and analytical technologies. Some peptide therapeutics has been derived from the venom gland of venomous animals, including snake, leech, lizard, snail, and scorpion. Scorpion was identified as a reservoir of important peptides with pharmaceutical properties. The scorpion uses these peptides for capturing prey and defense. However, their pharmacological properties in treating different diseases, including cardiac problems, autoimmune and infectious diseases, and diverse cancers, have been confirmed. Ion channel modifiers are the greatest components of the scorpion venom glands. Due to advances in proteomic and transcriptomic approaches, the identification of new scorpion venom peptides is steadily increasing. In this review, we tried to represent the current status of peptide medicines and describe the last peptide medications approved by FDA in 2022. Moreover, we will further explain potent peptides originating from scorpion venom, which have gone through important steps to be approved.
:肽是一种高效、选择性和相对安全的治疗方法。在过去的二十年里,由于鉴定、生产、修饰和分析技术的进步,天然肽已被获得、研究并最终获得美国食品药品监督管理局(FDA)的批准。一些肽疗法是从有毒动物的毒液腺中提取的,包括蛇、水蛭、蜥蜴、蜗牛和蝎子。蝎子被鉴定为具有药用性质的重要肽的库。蝎子利用这些肽来捕捉猎物和防御。然而,它们在治疗不同疾病方面的药理学特性,包括心脏问题、自身免疫性和传染性疾病以及各种癌症,已经得到证实。离子通道调节剂是蝎子毒液腺中最重要的成分。由于蛋白质组学和转录组学方法的进步,新的蝎毒肽的鉴定正在稳步增加。在这篇综述中,我们试图代表肽药物的现状,并描述2022年美国食品药品监督管理局批准的最后一种肽药物。此外,我们将进一步解释源自蝎毒的强效肽,这些肽已经经历了重要的步骤才能获得批准。
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引用次数: 0
In Vitro Anthelmintic Effect of Ferula assa-foetida Hydroalcoholic Extract Against Flukes of Fasciola hepatica and Dicrocoelium dendriticum 阿魏水酒精提取物对肝片形吸虫和树突双星吸虫的体外驱虫作用
IF 0.6 Q4 PHARMACOLOGY & PHARMACY Pub Date : 2023-02-13 DOI: 10.5812/jjnpp-133030
M. Arbabi, Atefeh Haddad, Monireh Esmaeli, Hossein Hooshyar, M. Sehat
Background: Dicrocoeliasis and fascioliasis are foodborne parasitic diseases of the biliary tract, resulting from Dicrocoelium dendriticum and Fasciola hepatica causing extensive financial losses and serious health problems in ruminants. Due to low-performance medications, drug delivery is a tremendous project to improve interventions available for these diseases. Objectives: This study aimed to determine the anthelmintic properties of Ferula assa-foetida extract against F. hepatica and D. dendriticum using in vitro assay. Methods: The effects of diverse concentrations of F. assa-foetida extract (400 - 1000 µg/mL) for 12-24 hours were examined for the treatment of D. dendriticum and F. hepatica. The anthelmintic efficacies were evaluated using scanning electron microscopy (SEM). The MTT assay was carried out to evaluate the cell viability of all cells in culture media. Results: The SEM images of treated worms by F. assa-foetida extract (200 µg/mL) confirmed excessive damage, which included an entire lack of sensory papillae and destruction of distinguished network structures and tegument vesicles. Variables of duration and concentration presented a considerable effect on both the mortality rate and the anthelmintic properties of F. assa-foetida; accordingly, as the time and concentration increased, the mortality rate became higher. Based on the MTT assay, the toxicity of F. assa-foetida at 800 µg/mL concentration was 8.7%. Therefore, it can be argued that F. assa-foetida had anthelmintic properties. Conclusions: This is the first study that evaluated the impact of F. assa-foetida on liver flukes of D. dendriticum and F. hepatica. Therefore, it paved the way for further studies on the control of those trematodes. It is recommended to document and look into the indigenous understanding of viable medicinal plants to provide evidence for their use.
背景:树枝状Dicrocoellium和肝片吸虫引起的胆道食源性寄生虫病,在反刍动物中造成广泛的经济损失和严重的健康问题。由于低性能药物,药物递送是一个巨大的项目,可以改善这些疾病的干预措施。目的:本研究旨在通过体外实验测定阿魏提取物对肝吸虫和树枝状D.dendrictum的驱虫性能。方法:研究不同浓度的鹅掌楸提取物(400-1000µg/mL)处理12-24小时对树枝状D.dendrictum和肝吸虫的影响。用扫描电子显微镜(SEM)评价了其驱虫效果。MTT法用于评估培养基中所有细胞的细胞活力。结果:用F.assa foetida提取物(200µg/mL)处理的蠕虫的SEM图像证实了过度损伤,包括完全缺乏感觉乳头以及显著的网络结构和被盖小泡的破坏。持续时间和浓度的变量对F.assa foetida的死亡率和驱虫性能都有相当大的影响;相应地,随着时间和浓度的增加,死亡率也变得更高。基于MTT法,在800µg/mL浓度下,F.assa foetida的毒性为8.7%。因此,可以认为F.assa foetida具有驱虫特性。结论:这是第一项评估鹅肝吸虫对树枝状和肝吸虫肝吸虫影响的研究。因此,它为进一步研究这些吸虫的控制铺平了道路。建议记录和调查土著人对可行药用植物的理解,为其使用提供证据。
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引用次数: 0
Anti-insect Potential of Pinus eldarica Leaf Essential Oil (PLE) Against Blattella germanica: German Cockroach 接骨木叶精油对德国小蠊的杀虫潜力
IF 0.6 Q4 PHARMACOLOGY & PHARMACY Pub Date : 2023-02-07 DOI: 10.5812/jjnpp-128983
Soheila Khorasani, A. Noori, L. Amjad, A. Mashayekh
Background: Pinus eldarica contains various components such as phenolic and terpene materials. Previous reports showed these two components had anti-insect properties. Pest control, especially for different species of cockroaches, is important because of transmission of various diseases. Objectives: The aim of this study was to determine the anti-insect potential of P. eldarica leaf essential oil (PLE) against Blattella germanica, German cockroach. Methods: The anti-insect activity of different doses (50, 150, and 300 μg/mL) of PLE of two areas of clean air (CA) zones and polluted air (PA) zones was evaluated by contact toxicity, fumigant toxicity, and repellence tests. Results: The results of contact test showed that the percentages of mortality rates were increased significantly in a dose- and time-dependent manner in both contact and fumigation methods as well as repellency percentage. Also, the results related to PA zones were more significant than CA zones. Conclusions: This study showed that essential leaf oil of P. eldarica induced mortality and repellency in German cockroach, and it can probably be used as a good candidate for pest control, in particular cockroaches.
背景:骨松含有多种成分,如酚类和萜烯类物质。以前的报道表明这两种成分具有抗虫性能。防治虫害,特别是防治不同种类的蟑螂,是很重要的,因为它们会传播各种疾病。目的:研究沉香叶精油(PLE)对德国小蠊、德国蜚蠊的抑虫作用。方法:通过接触毒性、熏蒸毒性和驱避试验,评价洁净空气区(CA)和污染空气区(PA)不同剂量(50、150、300 μg/mL)的PLE的抗虫活性。结果:接触试验结果表明,接触和熏蒸方法以及驱避率均呈剂量依赖性和时间依赖性,死亡率百分比均显著增加。此外,与PA区相关的结果比CA区更显著。结论:本研究表明,沉香叶精油对德国蜚蠊具有致死性和驱避性,可作为一种防治害虫,特别是蟑螂的良好候选药剂。
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引用次数: 0
Analysis of Some Putative Novel Peptides from Iranian Scorpion Venom Glands, Hemiscorpius lepturus, Using cDNA Library Construction 利用cDNA文库构建分析伊朗蝎毒腺中一些推测的新肽
IF 0.6 Q4 PHARMACOLOGY & PHARMACY Pub Date : 2023-02-04 DOI: 10.5812/jjnpp-133423
A. Jalali, M. Baradaran, H. Galehdari, Parnian Ghoncheh, Maryam Naderi Soorki
Background: To date, more than 300,000 distinct peptides have been identified in scorpion venom. However, only a limited number of these peptides have been described. Objectives: We characterized some putative peptides from the venom gland cDNA library of the Iranian yellow scorpion Hemiscorpius lepturus”. Methods: Total RNA was extracted from yellow Iranian scorpion glands. Single-stranded cDNA (sscDNA) and double-stranded cDNA (dscDNA) were synthesized by polymerase chain reaction (PCR). A cDNA library was achieved by inserting dscDNA into a special vector and subsequently transformed to chemically competent Escherichia coli as a host. The library was screened by culturing the liquid library on Lysogeny broth (LB)-agar plates. Analysis of positive clones was performed by plasmid extraction and the sequencing of the inserts. Finally, all cDNA sequences were analyzed and characterized by bioinformatics software. Results: One hundred colonies were randomly analyzed. Eighty-nine cDNA sequences had acceptable quality for bioinformatics analysis. Five sequences were selected for further analysis. The peptides related to these sequences were divided into two groups, non-disulfide bridge peptides (NDBP) and disulfide bridge peptides (DBP), the application of which in health and medical issues has been suggested. Conclusions: The data obtained in this study may be an important resource for further in vivo and in vitro functional assays to identify valuable therapeutic peptides.
背景:迄今为止,在蝎子毒液中已经鉴定出超过30万种不同的肽。然而,只有有限数量的这些肽被描述。目的:对伊朗黄蝎“半蝎”毒液腺cDNA文库中一些推测的多肽进行了鉴定。方法:提取黄蝎腺体总RNA。采用聚合酶链式反应(PCR)合成单链cDNA (sscDNA)和双链cDNA (dscDNA)。通过将dscDNA插入到一个特殊的载体中,然后将其转化为具有化学活性的大肠杆菌作为宿主,获得了cDNA文库。通过在溶根菌肉汤(LB)-琼脂平板上培养液体文库来筛选文库。对阳性克隆进行质粒提取和插入片段测序分析。最后,利用生物信息学软件对所有cDNA序列进行分析和表征。结果:随机分析100个菌落。89条cDNA序列质量可用于生物信息学分析。选取5个序列进行进一步分析。将与这些序列相关的肽分为非二硫桥肽(NDBP)和二硫桥肽(DBP)两类,并提出了它们在健康和医疗问题上的应用。结论:本研究获得的数据可能为进一步的体内和体外功能分析提供重要的资源,以鉴定有价值的治疗肽。
{"title":"Analysis of Some Putative Novel Peptides from Iranian Scorpion Venom Glands, Hemiscorpius lepturus, Using cDNA Library Construction","authors":"A. Jalali, M. Baradaran, H. Galehdari, Parnian Ghoncheh, Maryam Naderi Soorki","doi":"10.5812/jjnpp-133423","DOIUrl":"https://doi.org/10.5812/jjnpp-133423","url":null,"abstract":"Background: To date, more than 300,000 distinct peptides have been identified in scorpion venom. However, only a limited number of these peptides have been described. Objectives: We characterized some putative peptides from the venom gland cDNA library of the Iranian yellow scorpion Hemiscorpius lepturus”. Methods: Total RNA was extracted from yellow Iranian scorpion glands. Single-stranded cDNA (sscDNA) and double-stranded cDNA (dscDNA) were synthesized by polymerase chain reaction (PCR). A cDNA library was achieved by inserting dscDNA into a special vector and subsequently transformed to chemically competent Escherichia coli as a host. The library was screened by culturing the liquid library on Lysogeny broth (LB)-agar plates. Analysis of positive clones was performed by plasmid extraction and the sequencing of the inserts. Finally, all cDNA sequences were analyzed and characterized by bioinformatics software. Results: One hundred colonies were randomly analyzed. Eighty-nine cDNA sequences had acceptable quality for bioinformatics analysis. Five sequences were selected for further analysis. The peptides related to these sequences were divided into two groups, non-disulfide bridge peptides (NDBP) and disulfide bridge peptides (DBP), the application of which in health and medical issues has been suggested. Conclusions: The data obtained in this study may be an important resource for further in vivo and in vitro functional assays to identify valuable therapeutic peptides.","PeriodicalId":17745,"journal":{"name":"Jundishapur Journal of Natural Pharmaceutical Products","volume":" ","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47192056","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
An Overview of Cardiotonic Medicinal Plants from the Perspective of Iranian Traditional Medicine 从伊朗传统医学角度看养心药用植物
IF 0.6 Q4 PHARMACOLOGY & PHARMACY Pub Date : 2023-01-30 DOI: 10.5812/jjnpp-129338
A. Alembagheri, H. Hajimehdipoor, R. Choopani, S. Esmaeili
Context: Cardiovascular disorders are a leading cause of mortality and morbidity worldwide, especially in people with diabetes. Due to synthetic drugs’ adverse effects, new medicines are needed. Evidence Acquisition: Iranian traditional medicine (ITM) is one of the oldest medical systems. In this article, we first introduce a list of cardiotonic medicinal plants based on ITM. Then we review the cardio-related effects of these plants based on electronic databases. Results: Among the introduced medicinal plants from ITM, Phyllanthus emblica L., Rosa canina L., Ocimum basilicum L., and Melissa officinalis L. have cardiotonic effects. Also, P. emblica, O. basilicum, M. officinalis, Citrus medica L., Malus domestica Borkh., Elettaria cardamomum (L.) Maton, and R. canina have cardioprotective effects and possess several biological activities that reduce cardiovascular disease risk factors. Conclusions: The cardiotonic medicinal plants based on ITM have excellent value; several pharmacological studies have proved some of their cardioprotective and cardiotonic effects. The other plants’ potential for improving the heart’s contractile power as a cardiotonic drug must be evaluated in further pharmacological and clinical studies.
背景:心血管疾病是世界范围内死亡和发病的主要原因,尤其是糖尿病患者。由于合成药物的副作用,需要新的药物。证据获取:伊朗传统医学(ITM)是最古老的医疗系统之一。本文首先介绍了一种基于ITM的强心药用植物。在此基础上,对这些植物的心脏相关作用进行了综述。结果:引种的药用植物中,甘油三叶、犬牙蔷薇、罗勒和金莲具有强心作用。此外,还有枫香、basilicum、M. officinalis、Citrus medica L.、海棠。小豆蔻(L.)马顿和狗鼠具有心脏保护作用,并具有降低心血管疾病危险因素的几种生物活性。结论:以ITM为基础的强心药用植物具有优良的应用价值;一些药理学研究已经证明了它们的一些心脏保护和心脏强直作用。其他植物作为强心剂改善心脏收缩能力的潜力必须在进一步的药理学和临床研究中进行评估。
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引用次数: 0
Anti-diabetic Effects of Two Species of Eryngium on Streptozotocin-induced Diabetic Mice 两种枸杞对链脲佐菌素诱导的糖尿病小鼠的抗糖尿病作用
IF 0.6 Q4 PHARMACOLOGY & PHARMACY Pub Date : 2023-01-21 DOI: 10.5812/jjnpp-130824
H. Vaez, K. Hosseini, Vahideh Tarhriz, Shabnam Latifi, A. Garjani, P. Asgharian
Background: Eryngium is a genus with 274 species, which belongs to the Umbelliferae family. The nine species of this genus are endemic to Iran. The existence of terpenoids, saponins, flavonoids, and steroid components in most Eryngium species is the reason behind their anti-inflammatory, anti-cancer, and, especially, anti-diabetic effects among the well-known medicinal plants. Objectives: This study aimed to evaluate the effects of anti-diabetic features of Eryngium thyrsoideum and caucasicum on streptozotocin (STZ)-induced diabetic mice. Methods: The extract of the plants’ dried samples was prepared by using 70% ethanol solvent and adopting the maceration method. The mice were divided into eight groups, and the mice with a high blood glucose of more than 200 mg/dL were assigned to diabetic groups. Diabetic animals received different doses of the extract orally or through intraperitoneal (IP) injection for eighteen days. The animals were anesthetized by IP injection of ketamine and xylazine, and serum samples were isolated. Results: The results showed that hydroalcoholic extract of E. thyrsoideum significantly reduced the blood sugar level in diabetic groups receiving the extract (oral and IP) in low (100 mg/kg) and high (300 mg/kg) doses. Overall, oral glucose tolerance decreased significantly after two hours. Coincidental management of STZ and 300 mg/kg of the extract consumed orally reduced the blood sugar level remarkably. Serum malondialdehyde (MDA) and serum insulin levels significantly decreased and increased, respectively, in the diabetic groups receiving low and high doses (oral and IP) of the E. thyrsoideum extract. Conclusions: In sum, receiving 100 mg/kg and 300 mg/kg of hydroalcoholic extract (oral and IP) decreased the blood sugar level, while receiving oral glucose tolerance, serum MDA, and serum insulin caused a significant increase.
背景:伞属有274种,属于伞形科。这个属的9种是伊朗特有的。在众多知名的药用植物中,鸢尾属植物中萜类、皂苷类、黄酮类和甾类成分的存在是其具有抗炎、抗癌、特别是抗糖尿病作用的原因。目的:探讨胸腺苷白饮对链脲佐菌素(STZ)诱导的糖尿病小鼠的抗糖尿病作用。方法:以70%乙醇为溶剂,浸渍法制备植物干品提取物。将小鼠分为8组,将血糖高于200 mg/dL的小鼠分为糖尿病组。糖尿病动物口服或腹腔注射不同剂量的提取物18天。用氯胺酮和噻嗪注射液麻醉动物,并分离血清样本。结果:结果显示,低剂量(100 mg/kg)和高剂量(300 mg/kg)的糖尿病组(口服和口服)甲状腺素水醇提取物显著降低血糖水平。总的来说,口服葡萄糖耐量在两小时后显著下降。同时服用STZ和口服300 mg/kg提取物可显著降低血糖水平。在接受低剂量和高剂量(口服和口服)甲状腺素提取物的糖尿病组中,血清丙二醛(MDA)和血清胰岛素水平分别显著降低和升高。结论:综上所述,100 mg/kg和300 mg/kg水酒精提取物(口服和IP)可降低血糖水平,而口服糖耐量、血清丙二醛(MDA)和血清胰岛素显著升高。
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引用次数: 1
Althaea officinalis L. Extract Heals Skin Wounds in Second-Degree Burns in Mice 杜鹃花提取物对小鼠二度烧伤创面的修复作用
IF 0.6 Q4 PHARMACOLOGY & PHARMACY Pub Date : 2023-01-17 DOI: 10.5812/jjnpp-113667
M. Zabihi, Arefeh Shojaeemehr, A. Ranjbar, Mohammad Reza Rashidi Nooshabadi, Fatemeh Shishehbor, V. Ramezani
Background: Burns are one of the most common injuries; however, there are few medications to repair burned skin. Although antibacterial drugs such as topical silver sulfadiazine may prevent wound infections, they usually cannot accelerate skin healing. Marshmallow (Althaea officinalis L.) has long been used in Iranian traditional medicine to deal with skin burns. Objectives: The present study aimed to evaluate the effect of hydroalcoholic and aqueous extract of A. officinalis on the histopathologic parameters of second-degree burns in mice. Methods: The mice were randomly divided into nine groups (n = 6), including control and treatment groups. Experimental second-degree burns were induced on the dorsal skin of the mice by a metal plate (2 cm in diameter) under general anesthesia. Control and treatment groups were treated twice a day for 21 days topically. Normal saline was used for the control group, and hydroalcoholic and aqueous extracts of A. officinalis in three concentrations (1%, 2%, and 4%) were used for the treatment groups. All preparations were made in a hydrogel base. Finally, the histopathological parameters of the burned skin, including polymorphonuclear (PMN) migration, epithelialization, angiogenesis, and collagenization, were scored, and the total score of wound healing was calculated. Results: The findings showed that topical application of hydroalcoholic or aqueous extracts of A. officinalis accelerated the wound healing process. They reduced PMN migration, collagenization, epithelialization, and angiogenesis scores compared to the control group. There was a significant difference between the 4% hydroalcoholic extract group and the normal saline group. Conclusions: Althaea officinalis may have been used as an adjunct medication to accelerate wound healing in skin burns.
背景:烧伤是最常见的损伤之一;然而,很少有药物可以修复烧伤的皮肤。虽然抗菌药物如局部磺胺嘧啶银可以预防伤口感染,但它们通常不能加速皮肤愈合。棉花糖(Althaea officinalis L.)在伊朗传统医学中长期用于治疗皮肤烧伤。目的:研究马蹄草水提物和水提物对小鼠二度烧伤组织病理学指标的影响。方法:将小鼠随机分为9组(n = 6),分别为对照组和治疗组。在全身麻醉下,用直径2 cm的金属板对小鼠背部皮肤进行实验性二度烧伤。对照组和治疗组均给予局部治疗,每日2次,连用21 d。对照组采用生理盐水,治疗组采用三种浓度(1%、2%、4%)的马尾松水醇和水提液。所有的制剂都是在水凝胶基底中制备的。最后,对烧伤皮肤的多形核(PMN)迁移、上皮化、血管生成、胶原化等组织病理学参数进行评分,计算创面愈合总分。结果:局部应用马蹄草水醇提取物或水提物均能促进伤口愈合。与对照组相比,他们减少了PMN迁移、胶原、上皮化和血管生成评分。4%水酒精提取物组与生理盐水组有显著性差异。结论:杜鹃花可作为促进皮肤烧伤创面愈合的辅助用药。
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引用次数: 0
Green Tea Catechin Potentiating Doxorubicine Effects against BE(2)C Neuroblastoma Cells In Vitro 绿茶儿茶素增强阿霉素对BE(2)C神经母细胞瘤细胞的体外作用
IF 0.6 Q4 PHARMACOLOGY & PHARMACY Pub Date : 2023-01-13 DOI: 10.5812/jjnpp-129683
Z. Bakhtiari, L. Delphi, H. Sepehri, N. Motamed
Background: Neuroblastoma (NB), a malignant sympathetic nervous system cancer, is the second most common type of pediatric tumor. Increasing the number of NB death emerges to design a new strategy for NB treatment. Nowadays, the development of natural compounds has gradually increased due to their ability to apoptosis induction. Tea catechin, a flavonoid compound, is one of the natural combinations which inhibit tumor growth and enhance tumor cell apoptosis. In the current study, the effects of pure catechin, doxorubicin (DOX), and their combination on a cellular model of NB [BE(2)C cells] are perused. (NB) a malignant sympathetic nervous system cancer is the second most common type of pediatric tumor. Increasing the number of NB death emerges to design a new strategy for NB treatment. Nowadays, the development of natural compounds has gradually increased due to their ability to apoptosis induction. Tea catechin, a flavonoid compound, is one of the natural combinations which inhibit tumor growth and enhance tumor cell apoptosis. Objectives: In the current study, the effects of pure catechin, doxorubicin (DOX), and their combination on a cellular model of NB [BE(2)C cells] are perused. Methods: The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay was done to assess a response dose for each drug. Fluorescent Microscopic and cell cycle analyses were performed for apoptosis detection. Finally, Colony formation was performed to examine cell migration and invasion. Results: The MTT assay showed that catechin and DOX treatment inhibited the viability of the cells while the combination of their ineffective doses had more cytotoxic effects. However, these treatments could not inhibit the cell growth of the normal human fibroblast. Moreover, this combination reduced cell attachment, chromatin fragmentation, and G/S arrest in the cell cycle. The clonogenic assay demonstrated that colony size and numbers obviously reduced after ten days; therefore, Catchin and its combination with DOX suppressed cell capacities of clone formation and migration. Conclusions: These results suggest that catechin, DOX, and their combination may inhibit the proliferation, invasion, and migration of BE(2)C neuroblastoma cells in vitro while inducing cell apoptosis by arresting the cell cycle.
背景:神经母细胞瘤(NB)是一种恶性交感神经系统肿瘤,是第二常见的儿童肿瘤类型。增加NB死亡人数是设计NB治疗新策略的必要条件。目前,天然化合物因其诱导细胞凋亡的能力而逐渐得到开发。茶儿茶素是一种黄酮类化合物,是抑制肿瘤生长和促进肿瘤细胞凋亡的天然组合之一。在本研究中,研究了纯儿茶素、阿霉素(DOX)及其组合对NB [BE(2)C细胞]细胞模型的影响。恶性交感神经系统癌是儿童肿瘤的第二常见类型。增加NB死亡人数是设计NB治疗新策略的必要条件。目前,天然化合物因其诱导细胞凋亡的能力而逐渐得到开发。茶儿茶素是一种黄酮类化合物,是抑制肿瘤生长和促进肿瘤细胞凋亡的天然组合之一。目的:在本研究中,研究了纯儿茶素、阿霉素(DOX)及其联合使用对NB [BE(2)C细胞]细胞模型的影响。方法:采用3-(4,5-二甲基噻唑-2-基)-2,5-二苯基- 2h -溴化四唑(MTT)法测定各药物的反应剂量。荧光显微镜和细胞周期分析检测细胞凋亡。最后,进行菌落形成以检测细胞的迁移和侵袭。结果:MTT实验显示儿茶素和DOX处理均抑制细胞活力,无效剂量联合使用具有更强的细胞毒作用。然而,这些处理不能抑制正常人成纤维细胞的生长。此外,这种结合减少了细胞附着、染色质断裂和细胞周期中的G/S阻滞。克隆实验表明,10天后菌落大小和数量明显减少;因此,Catchin及其与DOX的结合抑制了细胞克隆形成和迁移的能力。结论:儿茶素、DOX及其联合使用可抑制体外BE(2)C神经母细胞瘤细胞的增殖、侵袭和迁移,并通过阻滞细胞周期诱导细胞凋亡。
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Jundishapur Journal of Natural Pharmaceutical Products
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