Setareh Dastyar Haghighi, Amal Khudair Khalaf, H. Mahmoudvand, A. Adineh, Ali Mohammad Maleki, Javad Ghasemian Yadegari
Background: The chemical agents applied to reduce the complications of hydatid cyst surgery are not free of side effects. Formononetin (FMN), as a natural isoflavone, has been shown to have various therapeutic benefits. Objectives: We studied the in vitro and ex vivo protoscolicidal activity and cellular mechanisms of FMN against hydatid cyst protoscoleces. Methods: Formononetin at 75, 150, and 300 μg/mL was mixed with hydatid cyst protoscoleces (103/mL), and the viability was determined in 5 to 60 min through eosin staining assay. The effect of FMN on caspase-3 activity was tested through a commercial colorimetric protease kit. In addition, the penetrability of the plasma membrane of protoscoleces after exposure to FMN was also determined by the SYTOX assay. Results: Formononetin at 300 μg/mL completely destroyed hydatid cyst protoscoleces after 30 minutes. While at 150 μg/mL, after 60 minutes of contact, 100% of protoscoleces were eliminated. By ex vivo assay, FMN showed its antiparasitic effect for longer periods. Based on these results, FMN at 300 μg/mL concentration completely eliminated hydatid cyst protoscoleces after 60 minutes of exposure. While at a concentration of 150 μg/mL after 60 minutes, 89.6% of protoscoleces were destroyed. Formononetin noticeably increased (P < 0.001) the activity of caspase-3 and the permeability of protoscoleces dose-dependently. Conclusions: Formononetin as a natural product showed promising effects on the protoscoleces of hydatid cysts, indicating that it can be considered a valuable scolicidal agent. However, additional investigations are necessary to evaluate its efficacy in animal models and human subjects.
{"title":"Antiparasitic Effects and Cellular Mechanisms of Formononetin (a Natural Isoflavone) Against Hydatid Cyst Protoscoleces","authors":"Setareh Dastyar Haghighi, Amal Khudair Khalaf, H. Mahmoudvand, A. Adineh, Ali Mohammad Maleki, Javad Ghasemian Yadegari","doi":"10.5812/jjnpp-129302","DOIUrl":"https://doi.org/10.5812/jjnpp-129302","url":null,"abstract":"Background: The chemical agents applied to reduce the complications of hydatid cyst surgery are not free of side effects. Formononetin (FMN), as a natural isoflavone, has been shown to have various therapeutic benefits. Objectives: We studied the in vitro and ex vivo protoscolicidal activity and cellular mechanisms of FMN against hydatid cyst protoscoleces. Methods: Formononetin at 75, 150, and 300 μg/mL was mixed with hydatid cyst protoscoleces (103/mL), and the viability was determined in 5 to 60 min through eosin staining assay. The effect of FMN on caspase-3 activity was tested through a commercial colorimetric protease kit. In addition, the penetrability of the plasma membrane of protoscoleces after exposure to FMN was also determined by the SYTOX assay. Results: Formononetin at 300 μg/mL completely destroyed hydatid cyst protoscoleces after 30 minutes. While at 150 μg/mL, after 60 minutes of contact, 100% of protoscoleces were eliminated. By ex vivo assay, FMN showed its antiparasitic effect for longer periods. Based on these results, FMN at 300 μg/mL concentration completely eliminated hydatid cyst protoscoleces after 60 minutes of exposure. While at a concentration of 150 μg/mL after 60 minutes, 89.6% of protoscoleces were destroyed. Formononetin noticeably increased (P < 0.001) the activity of caspase-3 and the permeability of protoscoleces dose-dependently. Conclusions: Formononetin as a natural product showed promising effects on the protoscoleces of hydatid cysts, indicating that it can be considered a valuable scolicidal agent. However, additional investigations are necessary to evaluate its efficacy in animal models and human subjects.","PeriodicalId":17745,"journal":{"name":"Jundishapur Journal of Natural Pharmaceutical Products","volume":" ","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49484024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Flavonoids in the Treatment of Glioblastoma Using Niosomal Nanocarrier","authors":"Mahmoud Gharbavi, A. Mojaddami","doi":"10.5812/jjnpp-135027","DOIUrl":"https://doi.org/10.5812/jjnpp-135027","url":null,"abstract":"<jats:p />","PeriodicalId":17745,"journal":{"name":"Jundishapur Journal of Natural Pharmaceutical Products","volume":" ","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42318716","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
: Peptides are highly potent, selective, and relatively safe therapeutics. Over the past two decades, natural peptides have been obtained, studied, and eventually approved by the Food and Drug Administration (FDA) due to advancements in identification, production, modification, and analytical technologies. Some peptide therapeutics has been derived from the venom gland of venomous animals, including snake, leech, lizard, snail, and scorpion. Scorpion was identified as a reservoir of important peptides with pharmaceutical properties. The scorpion uses these peptides for capturing prey and defense. However, their pharmacological properties in treating different diseases, including cardiac problems, autoimmune and infectious diseases, and diverse cancers, have been confirmed. Ion channel modifiers are the greatest components of the scorpion venom glands. Due to advances in proteomic and transcriptomic approaches, the identification of new scorpion venom peptides is steadily increasing. In this review, we tried to represent the current status of peptide medicines and describe the last peptide medications approved by FDA in 2022. Moreover, we will further explain potent peptides originating from scorpion venom, which have gone through important steps to be approved.
{"title":"Current Status of Peptide Medications and the Position of Active Therapeutic Peptides with Scorpion Venom Origin","authors":"M. Baradaran","doi":"10.5812/jjnpp-134049","DOIUrl":"https://doi.org/10.5812/jjnpp-134049","url":null,"abstract":": Peptides are highly potent, selective, and relatively safe therapeutics. Over the past two decades, natural peptides have been obtained, studied, and eventually approved by the Food and Drug Administration (FDA) due to advancements in identification, production, modification, and analytical technologies. Some peptide therapeutics has been derived from the venom gland of venomous animals, including snake, leech, lizard, snail, and scorpion. Scorpion was identified as a reservoir of important peptides with pharmaceutical properties. The scorpion uses these peptides for capturing prey and defense. However, their pharmacological properties in treating different diseases, including cardiac problems, autoimmune and infectious diseases, and diverse cancers, have been confirmed. Ion channel modifiers are the greatest components of the scorpion venom glands. Due to advances in proteomic and transcriptomic approaches, the identification of new scorpion venom peptides is steadily increasing. In this review, we tried to represent the current status of peptide medicines and describe the last peptide medications approved by FDA in 2022. Moreover, we will further explain potent peptides originating from scorpion venom, which have gone through important steps to be approved.","PeriodicalId":17745,"journal":{"name":"Jundishapur Journal of Natural Pharmaceutical Products","volume":" ","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44655512","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Arbabi, Atefeh Haddad, Monireh Esmaeli, Hossein Hooshyar, M. Sehat
Background: Dicrocoeliasis and fascioliasis are foodborne parasitic diseases of the biliary tract, resulting from Dicrocoelium dendriticum and Fasciola hepatica causing extensive financial losses and serious health problems in ruminants. Due to low-performance medications, drug delivery is a tremendous project to improve interventions available for these diseases. Objectives: This study aimed to determine the anthelmintic properties of Ferula assa-foetida extract against F. hepatica and D. dendriticum using in vitro assay. Methods: The effects of diverse concentrations of F. assa-foetida extract (400 - 1000 µg/mL) for 12-24 hours were examined for the treatment of D. dendriticum and F. hepatica. The anthelmintic efficacies were evaluated using scanning electron microscopy (SEM). The MTT assay was carried out to evaluate the cell viability of all cells in culture media. Results: The SEM images of treated worms by F. assa-foetida extract (200 µg/mL) confirmed excessive damage, which included an entire lack of sensory papillae and destruction of distinguished network structures and tegument vesicles. Variables of duration and concentration presented a considerable effect on both the mortality rate and the anthelmintic properties of F. assa-foetida; accordingly, as the time and concentration increased, the mortality rate became higher. Based on the MTT assay, the toxicity of F. assa-foetida at 800 µg/mL concentration was 8.7%. Therefore, it can be argued that F. assa-foetida had anthelmintic properties. Conclusions: This is the first study that evaluated the impact of F. assa-foetida on liver flukes of D. dendriticum and F. hepatica. Therefore, it paved the way for further studies on the control of those trematodes. It is recommended to document and look into the indigenous understanding of viable medicinal plants to provide evidence for their use.
{"title":"In Vitro Anthelmintic Effect of Ferula assa-foetida Hydroalcoholic Extract Against Flukes of Fasciola hepatica and Dicrocoelium dendriticum","authors":"M. Arbabi, Atefeh Haddad, Monireh Esmaeli, Hossein Hooshyar, M. Sehat","doi":"10.5812/jjnpp-133030","DOIUrl":"https://doi.org/10.5812/jjnpp-133030","url":null,"abstract":"Background: Dicrocoeliasis and fascioliasis are foodborne parasitic diseases of the biliary tract, resulting from Dicrocoelium dendriticum and Fasciola hepatica causing extensive financial losses and serious health problems in ruminants. Due to low-performance medications, drug delivery is a tremendous project to improve interventions available for these diseases. Objectives: This study aimed to determine the anthelmintic properties of Ferula assa-foetida extract against F. hepatica and D. dendriticum using in vitro assay. Methods: The effects of diverse concentrations of F. assa-foetida extract (400 - 1000 µg/mL) for 12-24 hours were examined for the treatment of D. dendriticum and F. hepatica. The anthelmintic efficacies were evaluated using scanning electron microscopy (SEM). The MTT assay was carried out to evaluate the cell viability of all cells in culture media. Results: The SEM images of treated worms by F. assa-foetida extract (200 µg/mL) confirmed excessive damage, which included an entire lack of sensory papillae and destruction of distinguished network structures and tegument vesicles. Variables of duration and concentration presented a considerable effect on both the mortality rate and the anthelmintic properties of F. assa-foetida; accordingly, as the time and concentration increased, the mortality rate became higher. Based on the MTT assay, the toxicity of F. assa-foetida at 800 µg/mL concentration was 8.7%. Therefore, it can be argued that F. assa-foetida had anthelmintic properties. Conclusions: This is the first study that evaluated the impact of F. assa-foetida on liver flukes of D. dendriticum and F. hepatica. Therefore, it paved the way for further studies on the control of those trematodes. It is recommended to document and look into the indigenous understanding of viable medicinal plants to provide evidence for their use.","PeriodicalId":17745,"journal":{"name":"Jundishapur Journal of Natural Pharmaceutical Products","volume":" ","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49110664","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Soheila Khorasani, A. Noori, L. Amjad, A. Mashayekh
Background: Pinus eldarica contains various components such as phenolic and terpene materials. Previous reports showed these two components had anti-insect properties. Pest control, especially for different species of cockroaches, is important because of transmission of various diseases. Objectives: The aim of this study was to determine the anti-insect potential of P. eldarica leaf essential oil (PLE) against Blattella germanica, German cockroach. Methods: The anti-insect activity of different doses (50, 150, and 300 μg/mL) of PLE of two areas of clean air (CA) zones and polluted air (PA) zones was evaluated by contact toxicity, fumigant toxicity, and repellence tests. Results: The results of contact test showed that the percentages of mortality rates were increased significantly in a dose- and time-dependent manner in both contact and fumigation methods as well as repellency percentage. Also, the results related to PA zones were more significant than CA zones. Conclusions: This study showed that essential leaf oil of P. eldarica induced mortality and repellency in German cockroach, and it can probably be used as a good candidate for pest control, in particular cockroaches.
{"title":"Anti-insect Potential of Pinus eldarica Leaf Essential Oil (PLE) Against Blattella germanica: German Cockroach","authors":"Soheila Khorasani, A. Noori, L. Amjad, A. Mashayekh","doi":"10.5812/jjnpp-128983","DOIUrl":"https://doi.org/10.5812/jjnpp-128983","url":null,"abstract":"Background: Pinus eldarica contains various components such as phenolic and terpene materials. Previous reports showed these two components had anti-insect properties. Pest control, especially for different species of cockroaches, is important because of transmission of various diseases. Objectives: The aim of this study was to determine the anti-insect potential of P. eldarica leaf essential oil (PLE) against Blattella germanica, German cockroach. Methods: The anti-insect activity of different doses (50, 150, and 300 μg/mL) of PLE of two areas of clean air (CA) zones and polluted air (PA) zones was evaluated by contact toxicity, fumigant toxicity, and repellence tests. Results: The results of contact test showed that the percentages of mortality rates were increased significantly in a dose- and time-dependent manner in both contact and fumigation methods as well as repellency percentage. Also, the results related to PA zones were more significant than CA zones. Conclusions: This study showed that essential leaf oil of P. eldarica induced mortality and repellency in German cockroach, and it can probably be used as a good candidate for pest control, in particular cockroaches.","PeriodicalId":17745,"journal":{"name":"Jundishapur Journal of Natural Pharmaceutical Products","volume":" ","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49015999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Jalali, M. Baradaran, H. Galehdari, Parnian Ghoncheh, Maryam Naderi Soorki
Background: To date, more than 300,000 distinct peptides have been identified in scorpion venom. However, only a limited number of these peptides have been described. Objectives: We characterized some putative peptides from the venom gland cDNA library of the Iranian yellow scorpion Hemiscorpius lepturus”. Methods: Total RNA was extracted from yellow Iranian scorpion glands. Single-stranded cDNA (sscDNA) and double-stranded cDNA (dscDNA) were synthesized by polymerase chain reaction (PCR). A cDNA library was achieved by inserting dscDNA into a special vector and subsequently transformed to chemically competent Escherichia coli as a host. The library was screened by culturing the liquid library on Lysogeny broth (LB)-agar plates. Analysis of positive clones was performed by plasmid extraction and the sequencing of the inserts. Finally, all cDNA sequences were analyzed and characterized by bioinformatics software. Results: One hundred colonies were randomly analyzed. Eighty-nine cDNA sequences had acceptable quality for bioinformatics analysis. Five sequences were selected for further analysis. The peptides related to these sequences were divided into two groups, non-disulfide bridge peptides (NDBP) and disulfide bridge peptides (DBP), the application of which in health and medical issues has been suggested. Conclusions: The data obtained in this study may be an important resource for further in vivo and in vitro functional assays to identify valuable therapeutic peptides.
{"title":"Analysis of Some Putative Novel Peptides from Iranian Scorpion Venom Glands, Hemiscorpius lepturus, Using cDNA Library Construction","authors":"A. Jalali, M. Baradaran, H. Galehdari, Parnian Ghoncheh, Maryam Naderi Soorki","doi":"10.5812/jjnpp-133423","DOIUrl":"https://doi.org/10.5812/jjnpp-133423","url":null,"abstract":"Background: To date, more than 300,000 distinct peptides have been identified in scorpion venom. However, only a limited number of these peptides have been described. Objectives: We characterized some putative peptides from the venom gland cDNA library of the Iranian yellow scorpion Hemiscorpius lepturus”. Methods: Total RNA was extracted from yellow Iranian scorpion glands. Single-stranded cDNA (sscDNA) and double-stranded cDNA (dscDNA) were synthesized by polymerase chain reaction (PCR). A cDNA library was achieved by inserting dscDNA into a special vector and subsequently transformed to chemically competent Escherichia coli as a host. The library was screened by culturing the liquid library on Lysogeny broth (LB)-agar plates. Analysis of positive clones was performed by plasmid extraction and the sequencing of the inserts. Finally, all cDNA sequences were analyzed and characterized by bioinformatics software. Results: One hundred colonies were randomly analyzed. Eighty-nine cDNA sequences had acceptable quality for bioinformatics analysis. Five sequences were selected for further analysis. The peptides related to these sequences were divided into two groups, non-disulfide bridge peptides (NDBP) and disulfide bridge peptides (DBP), the application of which in health and medical issues has been suggested. Conclusions: The data obtained in this study may be an important resource for further in vivo and in vitro functional assays to identify valuable therapeutic peptides.","PeriodicalId":17745,"journal":{"name":"Jundishapur Journal of Natural Pharmaceutical Products","volume":" ","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47192056","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Alembagheri, H. Hajimehdipoor, R. Choopani, S. Esmaeili
Context: Cardiovascular disorders are a leading cause of mortality and morbidity worldwide, especially in people with diabetes. Due to synthetic drugs’ adverse effects, new medicines are needed. Evidence Acquisition: Iranian traditional medicine (ITM) is one of the oldest medical systems. In this article, we first introduce a list of cardiotonic medicinal plants based on ITM. Then we review the cardio-related effects of these plants based on electronic databases. Results: Among the introduced medicinal plants from ITM, Phyllanthus emblica L., Rosa canina L., Ocimum basilicum L., and Melissa officinalis L. have cardiotonic effects. Also, P. emblica, O. basilicum, M. officinalis, Citrus medica L., Malus domestica Borkh., Elettaria cardamomum (L.) Maton, and R. canina have cardioprotective effects and possess several biological activities that reduce cardiovascular disease risk factors. Conclusions: The cardiotonic medicinal plants based on ITM have excellent value; several pharmacological studies have proved some of their cardioprotective and cardiotonic effects. The other plants’ potential for improving the heart’s contractile power as a cardiotonic drug must be evaluated in further pharmacological and clinical studies.
{"title":"An Overview of Cardiotonic Medicinal Plants from the Perspective of Iranian Traditional Medicine","authors":"A. Alembagheri, H. Hajimehdipoor, R. Choopani, S. Esmaeili","doi":"10.5812/jjnpp-129338","DOIUrl":"https://doi.org/10.5812/jjnpp-129338","url":null,"abstract":"Context: Cardiovascular disorders are a leading cause of mortality and morbidity worldwide, especially in people with diabetes. Due to synthetic drugs’ adverse effects, new medicines are needed. Evidence Acquisition: Iranian traditional medicine (ITM) is one of the oldest medical systems. In this article, we first introduce a list of cardiotonic medicinal plants based on ITM. Then we review the cardio-related effects of these plants based on electronic databases. Results: Among the introduced medicinal plants from ITM, Phyllanthus emblica L., Rosa canina L., Ocimum basilicum L., and Melissa officinalis L. have cardiotonic effects. Also, P. emblica, O. basilicum, M. officinalis, Citrus medica L., Malus domestica Borkh., Elettaria cardamomum (L.) Maton, and R. canina have cardioprotective effects and possess several biological activities that reduce cardiovascular disease risk factors. Conclusions: The cardiotonic medicinal plants based on ITM have excellent value; several pharmacological studies have proved some of their cardioprotective and cardiotonic effects. The other plants’ potential for improving the heart’s contractile power as a cardiotonic drug must be evaluated in further pharmacological and clinical studies.","PeriodicalId":17745,"journal":{"name":"Jundishapur Journal of Natural Pharmaceutical Products","volume":" ","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43148880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
H. Vaez, K. Hosseini, Vahideh Tarhriz, Shabnam Latifi, A. Garjani, P. Asgharian
Background: Eryngium is a genus with 274 species, which belongs to the Umbelliferae family. The nine species of this genus are endemic to Iran. The existence of terpenoids, saponins, flavonoids, and steroid components in most Eryngium species is the reason behind their anti-inflammatory, anti-cancer, and, especially, anti-diabetic effects among the well-known medicinal plants. Objectives: This study aimed to evaluate the effects of anti-diabetic features of Eryngium thyrsoideum and caucasicum on streptozotocin (STZ)-induced diabetic mice. Methods: The extract of the plants’ dried samples was prepared by using 70% ethanol solvent and adopting the maceration method. The mice were divided into eight groups, and the mice with a high blood glucose of more than 200 mg/dL were assigned to diabetic groups. Diabetic animals received different doses of the extract orally or through intraperitoneal (IP) injection for eighteen days. The animals were anesthetized by IP injection of ketamine and xylazine, and serum samples were isolated. Results: The results showed that hydroalcoholic extract of E. thyrsoideum significantly reduced the blood sugar level in diabetic groups receiving the extract (oral and IP) in low (100 mg/kg) and high (300 mg/kg) doses. Overall, oral glucose tolerance decreased significantly after two hours. Coincidental management of STZ and 300 mg/kg of the extract consumed orally reduced the blood sugar level remarkably. Serum malondialdehyde (MDA) and serum insulin levels significantly decreased and increased, respectively, in the diabetic groups receiving low and high doses (oral and IP) of the E. thyrsoideum extract. Conclusions: In sum, receiving 100 mg/kg and 300 mg/kg of hydroalcoholic extract (oral and IP) decreased the blood sugar level, while receiving oral glucose tolerance, serum MDA, and serum insulin caused a significant increase.
{"title":"Anti-diabetic Effects of Two Species of Eryngium on Streptozotocin-induced Diabetic Mice","authors":"H. Vaez, K. Hosseini, Vahideh Tarhriz, Shabnam Latifi, A. Garjani, P. Asgharian","doi":"10.5812/jjnpp-130824","DOIUrl":"https://doi.org/10.5812/jjnpp-130824","url":null,"abstract":"Background: Eryngium is a genus with 274 species, which belongs to the Umbelliferae family. The nine species of this genus are endemic to Iran. The existence of terpenoids, saponins, flavonoids, and steroid components in most Eryngium species is the reason behind their anti-inflammatory, anti-cancer, and, especially, anti-diabetic effects among the well-known medicinal plants. Objectives: This study aimed to evaluate the effects of anti-diabetic features of Eryngium thyrsoideum and caucasicum on streptozotocin (STZ)-induced diabetic mice. Methods: The extract of the plants’ dried samples was prepared by using 70% ethanol solvent and adopting the maceration method. The mice were divided into eight groups, and the mice with a high blood glucose of more than 200 mg/dL were assigned to diabetic groups. Diabetic animals received different doses of the extract orally or through intraperitoneal (IP) injection for eighteen days. The animals were anesthetized by IP injection of ketamine and xylazine, and serum samples were isolated. Results: The results showed that hydroalcoholic extract of E. thyrsoideum significantly reduced the blood sugar level in diabetic groups receiving the extract (oral and IP) in low (100 mg/kg) and high (300 mg/kg) doses. Overall, oral glucose tolerance decreased significantly after two hours. Coincidental management of STZ and 300 mg/kg of the extract consumed orally reduced the blood sugar level remarkably. Serum malondialdehyde (MDA) and serum insulin levels significantly decreased and increased, respectively, in the diabetic groups receiving low and high doses (oral and IP) of the E. thyrsoideum extract. Conclusions: In sum, receiving 100 mg/kg and 300 mg/kg of hydroalcoholic extract (oral and IP) decreased the blood sugar level, while receiving oral glucose tolerance, serum MDA, and serum insulin caused a significant increase.","PeriodicalId":17745,"journal":{"name":"Jundishapur Journal of Natural Pharmaceutical Products","volume":"1 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41599421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Zabihi, Arefeh Shojaeemehr, A. Ranjbar, Mohammad Reza Rashidi Nooshabadi, Fatemeh Shishehbor, V. Ramezani
Background: Burns are one of the most common injuries; however, there are few medications to repair burned skin. Although antibacterial drugs such as topical silver sulfadiazine may prevent wound infections, they usually cannot accelerate skin healing. Marshmallow (Althaea officinalis L.) has long been used in Iranian traditional medicine to deal with skin burns. Objectives: The present study aimed to evaluate the effect of hydroalcoholic and aqueous extract of A. officinalis on the histopathologic parameters of second-degree burns in mice. Methods: The mice were randomly divided into nine groups (n = 6), including control and treatment groups. Experimental second-degree burns were induced on the dorsal skin of the mice by a metal plate (2 cm in diameter) under general anesthesia. Control and treatment groups were treated twice a day for 21 days topically. Normal saline was used for the control group, and hydroalcoholic and aqueous extracts of A. officinalis in three concentrations (1%, 2%, and 4%) were used for the treatment groups. All preparations were made in a hydrogel base. Finally, the histopathological parameters of the burned skin, including polymorphonuclear (PMN) migration, epithelialization, angiogenesis, and collagenization, were scored, and the total score of wound healing was calculated. Results: The findings showed that topical application of hydroalcoholic or aqueous extracts of A. officinalis accelerated the wound healing process. They reduced PMN migration, collagenization, epithelialization, and angiogenesis scores compared to the control group. There was a significant difference between the 4% hydroalcoholic extract group and the normal saline group. Conclusions: Althaea officinalis may have been used as an adjunct medication to accelerate wound healing in skin burns.
{"title":"Althaea officinalis L. Extract Heals Skin Wounds in Second-Degree Burns in Mice","authors":"M. Zabihi, Arefeh Shojaeemehr, A. Ranjbar, Mohammad Reza Rashidi Nooshabadi, Fatemeh Shishehbor, V. Ramezani","doi":"10.5812/jjnpp-113667","DOIUrl":"https://doi.org/10.5812/jjnpp-113667","url":null,"abstract":"Background: Burns are one of the most common injuries; however, there are few medications to repair burned skin. Although antibacterial drugs such as topical silver sulfadiazine may prevent wound infections, they usually cannot accelerate skin healing. Marshmallow (Althaea officinalis L.) has long been used in Iranian traditional medicine to deal with skin burns. Objectives: The present study aimed to evaluate the effect of hydroalcoholic and aqueous extract of A. officinalis on the histopathologic parameters of second-degree burns in mice. Methods: The mice were randomly divided into nine groups (n = 6), including control and treatment groups. Experimental second-degree burns were induced on the dorsal skin of the mice by a metal plate (2 cm in diameter) under general anesthesia. Control and treatment groups were treated twice a day for 21 days topically. Normal saline was used for the control group, and hydroalcoholic and aqueous extracts of A. officinalis in three concentrations (1%, 2%, and 4%) were used for the treatment groups. All preparations were made in a hydrogel base. Finally, the histopathological parameters of the burned skin, including polymorphonuclear (PMN) migration, epithelialization, angiogenesis, and collagenization, were scored, and the total score of wound healing was calculated. Results: The findings showed that topical application of hydroalcoholic or aqueous extracts of A. officinalis accelerated the wound healing process. They reduced PMN migration, collagenization, epithelialization, and angiogenesis scores compared to the control group. There was a significant difference between the 4% hydroalcoholic extract group and the normal saline group. Conclusions: Althaea officinalis may have been used as an adjunct medication to accelerate wound healing in skin burns.","PeriodicalId":17745,"journal":{"name":"Jundishapur Journal of Natural Pharmaceutical Products","volume":" ","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47962493","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background: Neuroblastoma (NB), a malignant sympathetic nervous system cancer, is the second most common type of pediatric tumor. Increasing the number of NB death emerges to design a new strategy for NB treatment. Nowadays, the development of natural compounds has gradually increased due to their ability to apoptosis induction. Tea catechin, a flavonoid compound, is one of the natural combinations which inhibit tumor growth and enhance tumor cell apoptosis. In the current study, the effects of pure catechin, doxorubicin (DOX), and their combination on a cellular model of NB [BE(2)C cells] are perused. (NB) a malignant sympathetic nervous system cancer is the second most common type of pediatric tumor. Increasing the number of NB death emerges to design a new strategy for NB treatment. Nowadays, the development of natural compounds has gradually increased due to their ability to apoptosis induction. Tea catechin, a flavonoid compound, is one of the natural combinations which inhibit tumor growth and enhance tumor cell apoptosis. Objectives: In the current study, the effects of pure catechin, doxorubicin (DOX), and their combination on a cellular model of NB [BE(2)C cells] are perused. Methods: The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay was done to assess a response dose for each drug. Fluorescent Microscopic and cell cycle analyses were performed for apoptosis detection. Finally, Colony formation was performed to examine cell migration and invasion. Results: The MTT assay showed that catechin and DOX treatment inhibited the viability of the cells while the combination of their ineffective doses had more cytotoxic effects. However, these treatments could not inhibit the cell growth of the normal human fibroblast. Moreover, this combination reduced cell attachment, chromatin fragmentation, and G/S arrest in the cell cycle. The clonogenic assay demonstrated that colony size and numbers obviously reduced after ten days; therefore, Catchin and its combination with DOX suppressed cell capacities of clone formation and migration. Conclusions: These results suggest that catechin, DOX, and their combination may inhibit the proliferation, invasion, and migration of BE(2)C neuroblastoma cells in vitro while inducing cell apoptosis by arresting the cell cycle.
{"title":"Green Tea Catechin Potentiating Doxorubicine Effects against BE(2)C Neuroblastoma Cells In Vitro","authors":"Z. Bakhtiari, L. Delphi, H. Sepehri, N. Motamed","doi":"10.5812/jjnpp-129683","DOIUrl":"https://doi.org/10.5812/jjnpp-129683","url":null,"abstract":"Background: Neuroblastoma (NB), a malignant sympathetic nervous system cancer, is the second most common type of pediatric tumor. Increasing the number of NB death emerges to design a new strategy for NB treatment. Nowadays, the development of natural compounds has gradually increased due to their ability to apoptosis induction. Tea catechin, a flavonoid compound, is one of the natural combinations which inhibit tumor growth and enhance tumor cell apoptosis. In the current study, the effects of pure catechin, doxorubicin (DOX), and their combination on a cellular model of NB [BE(2)C cells] are perused. (NB) a malignant sympathetic nervous system cancer is the second most common type of pediatric tumor. Increasing the number of NB death emerges to design a new strategy for NB treatment. Nowadays, the development of natural compounds has gradually increased due to their ability to apoptosis induction. Tea catechin, a flavonoid compound, is one of the natural combinations which inhibit tumor growth and enhance tumor cell apoptosis. Objectives: In the current study, the effects of pure catechin, doxorubicin (DOX), and their combination on a cellular model of NB [BE(2)C cells] are perused. Methods: The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay was done to assess a response dose for each drug. Fluorescent Microscopic and cell cycle analyses were performed for apoptosis detection. Finally, Colony formation was performed to examine cell migration and invasion. Results: The MTT assay showed that catechin and DOX treatment inhibited the viability of the cells while the combination of their ineffective doses had more cytotoxic effects. However, these treatments could not inhibit the cell growth of the normal human fibroblast. Moreover, this combination reduced cell attachment, chromatin fragmentation, and G/S arrest in the cell cycle. The clonogenic assay demonstrated that colony size and numbers obviously reduced after ten days; therefore, Catchin and its combination with DOX suppressed cell capacities of clone formation and migration. Conclusions: These results suggest that catechin, DOX, and their combination may inhibit the proliferation, invasion, and migration of BE(2)C neuroblastoma cells in vitro while inducing cell apoptosis by arresting the cell cycle.","PeriodicalId":17745,"journal":{"name":"Jundishapur Journal of Natural Pharmaceutical Products","volume":" ","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48843689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}