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Riparian Tree Vegetation Diversity Salah Nama Island in Banyuasin, South Sumatera 南苏门答腊Banyuasin的Salah Nama岛河岸树木植被多样性
Q4 Agricultural and Biological Sciences Pub Date : 2021-07-03 DOI: 10.20473/JIPK.V13I2.26476
M. Dwirastina, E. Riani, Agnes Puspita Sudarmo
Highlight Research:Riparian plants play an important role in maintaining the balance of an aquatic ecosystem.The diversity of riparian aquatic plants on the island of Salah Nama BanyuasinData collection on riparian plants used the quadratic transect methodThe results showed that there were 21 types of riparian aquatic plants in 16 families.The highest composition is in the Lythraceae family, namely Sonneratia acidaAbstractRiparian plants play an important role in maintaining the balance of an aquatic ecosystem. The missing plant components from a water can cause sedimentation and change the microhabitat in these waters. The purpose of the study is to determine the diversity of riparian aquatic plants on the island of Salah Nama Banyuasin. The research was conducted in August 2016, November 2016, and January 2017 at Salah Nama Island Mariana Ilir Banyuasin 1, South Sumatra. Sampling was done by using the purposive sampling method. Determination of the sampling location used GPS (Global positioning system). Observation stations consisted of 5 sampling locations. The method of taking water plants was done in exploratory way. Data collection on riparian plants used the quadratic transect method measuring 1 m x 1 m. Samples of riparian plants were taken then wrapped with newspaper or paper and put into large plastic, labeled then taken to the testing laboratory Institute Inland fisheries and extension, Palembang. The results showed that there were 21 types of riparian aquatic plants in 16 families. The highest composition is in the Lythraceae family, namely Sonneratia acida. The diversity index of aquatic plants ranges from 1
亮点研究:河岸植物在维持水生生态系统平衡方面发挥着重要作用。Salah Nama Banyuasin岛河岸水生植物的多样性利用二次样条法对河岸水生植物进行数据采集,结果表明,共有16科21种河岸水生植物。最高的成分是Lythraceae科,即Sonneratia acidaAbstractRiparian植物在维持水生生态系统的平衡方面发挥着重要作用。水中缺失的植物成分会导致沉积,并改变这些水中的微栖息地。本研究的目的是确定Salah Nama Banyuasin岛河岸水生植物的多样性。该研究于2016年8月、2016年11月和2017年1月在南苏门答腊岛的萨拉赫纳马岛Mariana Ilir Banyuasin 1进行。采用有目的的抽样方法进行抽样。使用GPS(全球定位系统)确定采样位置。观测站由5个采样点组成。取水草的方法是探索性的。河岸植物的数据收集采用了测量1 m x 1 m的二次样带法。河岸植物的样本用报纸或纸包裹,放入大塑料中,贴上标签,然后送往巨港内陆渔业和扩展研究所的测试实验室。结果表明,河岸水生植物共有16科21种。最高的成分是在Lythraceae科,即Sonneratia acida。水生植物的多样性指数在稳定条件类别中为1<H’<3。根据研究结果,占主导地位的河岸植物种类为桑拿蒂藻。可以得出的结论是,萨拉赫纳马岛河岸植物的多样性属于中等类别,占主导的河岸植物为桑拿蒂藻。
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引用次数: 0
Population Dynamics of Bullet Tuna (Auxis rochei Risso 1881) from the Indian Ocean, West Nusa Tenggara, Indonesia 印度尼西亚西努沙登加拉印度洋子弹金枪鱼种群动态
Q4 Agricultural and Biological Sciences Pub Date : 2021-06-24 DOI: 10.20473/JIPK.V13I2.26017
E. Asrial, R. I. Khasanah, Fathurriadi Fathrurriadi, Yasir Arapat, Usma Kurniawan Hadi, L. A. Kalih, Lalu Samsul Rizal, M. A. Liliyanti, E. Rosadi, D. Setyohadi, M. Junaidi, I. Rathnayake
Highlight Research:The bullet tuna (Auxis rochei) was identified and analysed.The length at first maturity (Lm) of bullet tuna (Auxis rochei) was analyzedThe eligibility status of bullet tuna (Auxis rochei) has been analyzed and estimatedThe LWR curve can be used to estimate age groups of fish compared to the sigmoid curveThe CF cannot be automatically used to estimate fish worth selling AbstractThe bullet tuna (Auxis rochei), BLT or tongkol lisong (Indonesian) is a species of neritic tuna which is one of the target fish for small scale fishers. The problem of bullet tuna fisheries was that it has reached overexploitation and the immature bullet tuna are still caught. This study aimed to assess the population dynamics of the BLT and its size composition caught by small scale fishers in the Indian Ocean, West Nusa Tenggara area was carried out in Bangko-Bangko (West Lombok), Cemplung Beach (South Sumbawa) and Tanjung Luar Fishing Port (East Lombok) between July 2019 and June 2020. The data was derived from the measurement and weighing results of 1,217 BLT collected from the respective collector traders at each sampling location. The growth pattern of BLT was isometric () which meant the ideal body shape (mesomorph) and length at first maturity (FLm) was 31.11 cm. The length-weight relationship curve showed that BLT was in an immature age group (adolescent fish). Meanwhile, the composition of BLT is worth selling (Condition Factor > 1.00), fit for catch (FLc / FLm > 1.00) and worthy of maturity (FLc > FLm), respectively 99.92%, 0.82% and 0.00%. The BLT fish composition indicates that the stocks of resources and populations do not support current sustainable fisheries management.
重点研究:子弹金枪鱼(Auxis rochei)的鉴定和分析。本文对子弹金枪鱼的初成熟长度(Lm)进行了分析和估计,与s型曲线相比,LWR曲线可以用来估计鱼类的年龄组,但CF不能自动用于估计鱼类的销售价值。摘要子弹金枪鱼,BLT或tongkol lisong(印度尼西亚)是一种浅海金枪鱼,是小型渔民的目标鱼之一。子弹金枪鱼渔业的问题是,它已经达到了过度开发,未成熟的子弹金枪鱼仍然被捕获。本研究于2019年7月至2020年6月在曼谷- bangko(西龙目岛)、Cemplung海滩(南松巴哇)和Tanjung Luar渔港(东龙目岛)进行,旨在评估印度洋西努沙登加拉地区小规模渔民捕获的BLT种群动态及其大小组成。数据是根据在每个采样地点从各自的收集商处收集的1,217个BLT的测量和称重结果得出的。BLT的生长模式为等长(),即理想体型(mesomorph)和初熟长度(FLm)为31.11 cm。长度-重量关系曲线显示,BLT是在一个不成熟的年龄组(青春期鱼)。同时,BLT的构成值得出售(Condition Factor >.00)、适合捕捞(FLc / FLm > 1.00)和值得成熟(FLc > FLm)的比例分别为99.92%、0.82%和0.00%。BLT鱼类组成表明,资源和种群存量不支持当前的可持续渔业管理。
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引用次数: 0
Exploration of Antimicrobial Potency of Mangrove Symbiont Against Multi-Drug Resistant Bacteria 红树林共生体对多药耐药菌的抗菌作用探讨
Q4 Agricultural and Biological Sciences Pub Date : 2021-06-24 DOI: 10.20473/JIPK.V13I2.26199
D. Pringgenies, W. A. Setyati, Ali Djunaedi, Rini Pramesti, S. Rudiyanti, D. Ariyanto
Highlight ResearchAntimicrobial potential against the test microbesRhizhopora mucronata isolate showed 95% homology with Bacillus subtilis, and 97% homology with Bacillus oceanisediminis,Acanthus ilicifolius isolate showed 96% homology with Paracoccus caeni, and 89% homology with Bacillus circulans. The study found 4 isolates with antimicrobial potency against MDR pathogenic microbes.The symbiont microbes taken from Rhizophora mucronata and Acanthus ilicifolius were determined to be of the genus Bacillus and Paracoccus AbstractAntimicrobial property of mangrove symbiont have the ability to fight Multi Drug Resistant bacteria which were Staphylococcus aureus, Escherichia coli, and Vibrio haryeyi. This study aimed to determine the potential of symbiont microbes from the root of Rhizopora mucronata and Acanthus iilicifolius as antimicrobial agents against multi-drug resistant (MDR) pathogenic microbes. This research was conducted during July to November 2020. The MDR bacteria were S. aureus, E. coli, and V. harveyi MDR test microbes. The symbiont microbes were identified through molecular analyses (PCR 16S rDNA). Isolation of symbiont microbes from R. mucronata resulted in 16 isolates, while isolation from A. iilicifolius resulted in 14 isolates. Based on the antimicrobial qualitative test against S. aureus, 8 out of 16 microbial isolates from R. mucronata were found to show antimicrobial properties. The testing of A. ilicifolius symbiont microbes against S. aureus showed 8 out of 14 isolates with antimicrobial properties. The test against E. coli resulted in 2 out of 16 microbial isolates from R. mucronata and 5 out of 14 isolates from A. ilicifolius with antimicrobial properties. The test against V. harveyi resulted in two out of 16 microbial isolates from R.mucronata and 4 out of 14 isolates from A. ilicifolius with antimicrobial properties. The quantitative test found 2 isolates from R. mucronta, namely isolates RM10 and RM12, with antimicrobial properties against MDR strain E. coli, with the best isolate being RM10, which produced 11.22 mm of inhibition zone diameter. Furthermore, the selection of isolates was based on the size of the inhibition zone, the clearness of the inhibition zone and the potential for antibacterial activity. Based on their overall antimicrobial potential against the test microbes, four isolates were selected.  Molecular analyses of RM12 isolate showed 95% homology with Bacillus subtilis, of RM 10 isolate showed 97% homology with Bacillus oceanisediminis, of AC isolate showed 96% homology with Paracoccus caeni, and of AC 5 isolate showed 89% homology with Bacillus circulans. The study found four isolates with antimicrobial potency against MDR pathogenic microbes. The symbiont microbes taken from R. mucronata and A. ilicifolius were determined to be of the genus Bacillus and Paracoccus. 
重点研究对试验微生物的抑菌潜力:粗根孢杆菌与枯草芽孢杆菌的同源性为95%,与海洋芽孢杆菌的同源性为97%,与caeni副球菌的同源性为96%,与环状芽孢杆菌的同源性为89%。研究发现4株菌株对耐多药病原菌具有抗菌效力。摘要红树林共生体具有抗多种耐药菌金黄色葡萄球菌、大肠埃希菌和哈里伊弧菌的能力。本研究旨在确定毛霉根菌和棘豆根中共生微生物作为抗多药耐药(MDR)病原菌的潜力。该研究于2020年7月至11月进行。耐多药试验菌为金黄色葡萄球菌、大肠杆菌和哈维氏弧菌。通过分子PCR (16S rDNA)鉴定共生体微生物。从长柄霉中分离到16株,从鹅毛霉中分离到14株。通过对金黄色葡萄球菌的抑菌定性试验,发现16株真菌中有8株具有抑菌活性。结果表明,14株菌株中8株对金黄色葡萄球菌具有抑菌活性。对大肠杆菌的抑菌试验结果表明,16株麻霉分离株中有2株具有抑菌活性,14株青枝芽孢杆菌分离株中有5株具有抑菌活性。对哈维伊弧菌的抑菌试验结果表明,16株麻霉分离株中有2株具有抑菌活性,14株青枝芽孢杆菌分离株中有4株具有抑菌活性。定量试验发现,2株mucronta分离株RM10和RM12对MDR菌株大肠杆菌具有抑菌性能,其中RM10抑菌效果最好,抑菌带直径为11.22 mm。此外,菌株的选择还基于抑菌带的大小、抑菌带的清晰度和抑菌活性的潜力。根据对试验微生物的综合抑菌潜力,筛选出4株分离菌。RM12与枯草芽孢杆菌的同源性为95%,rm10与海洋芽孢杆菌的同源性为97%,AC与caeni副球菌的同源性为96%,AC 5与环状芽孢杆菌的同源性为89%。该研究发现四株分离株具有抗多药耐药病原菌的抗菌效力。结果表明,从毛孢霉和毛孢霉中分离出的共生微生物分别为芽孢杆菌属和副球菌属。
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引用次数: 7
Preliminary Identification to Local Coral Bleaching Event in Manjuto Beach, Pesisir Selatan Regency, West Sumatra: Hydro-Oceanographic Perspectives 西苏门答腊Pesisir Selatan reggency Manjuto海滩局部珊瑚白化事件的初步鉴定:水文海洋学观点
Q4 Agricultural and Biological Sciences Pub Date : 2021-06-23 DOI: 10.20473/JIPK.V13I2.25502
U. J. Wisha, R. Dhiauddin, G. A. Rahmawan, Y. J. Wijaya
Highlight ResarchThe cause of local coral bleaching in Manjuto Beash has been addressed.The influence of ebb-tide cycles on salinity mixing and stratification was analyzed.Spatial analysis Digital Shoreline Analysis System (DSASv5) was conducted to determine the coastline changes in Manjuto Beach.Flow model flexible mesh was simulated to determine the flow pattern within Sungai Pinang Bay.AbstractIn October 2019, the local community reported the occurrence of coral bleaching of a colony of Acropora sp. at Manjuto Beach, Pesisir Selatan Regency experienced bleaching. It was published in several local news, becoming a trending topic among local and central government authorities and coastal communities. There were many inaccuracies about the cause of this phenomenon. This study aimed to identify the causes of local coral bleaching in Manjuto Beach based on oceanographic perspectives. The water quality data collected using TOA DKK water quality checker in the surrounding Manjuto Beach were assessed descriptive-statistically. This study also analyzed the spatial changes of the coastline using DSASv5. A time series of tidal data was also used to analyze the tidal range-induced salinity stratification. A flow model with a flexible mesh was also simulated to determine the water mass movement and longshore current patterns in Manjuto Beach. Dissolved oxygen (DO), temperature, and salinity showed anomalies compared to the water quality standard to support marine life. During both flood and ebb tides, it ranged from 5.8-11.2 mg/L, 28-28.3oC, and 25-28 o/oo, respectively. The other parameters measured (pH, conductivity, turbidity, and density) were suitable for marine biota. The findings show that tidal range has a unique influence on salinity stratification. The intrusion of groundwater supply resulted in lowering of salinity, inducing local coral bleaching in Manjuto Beach. Changes in salinity levels were also triggered by tidal current ranging from 0-0.31 m/s resulting in cumulative salinity shock. Currently, Manjuto Beach is experiencing accretion ranging from 2.36-3.17 m/year, altering the water coverage through the flood-ebb cycles. Those states cause cumulative sun rays’ exposures and salinity shock induced by flood-ebb cycles. That is why local coral bleaching event is undoubtedly avoided.
亮点研究Manjuto Beash当地珊瑚白化的原因已经得到解决。分析了退潮周期对盐度混合和分层的影响。空间分析数字海岸线分析系统(DSASv5)用于确定曼朱托海滩的海岸线变化。模拟流动模型柔性网格以确定Sungai Pinang Bay内的流动模式。摘要2019年10月,当地社区报告曼朱托滩Acropora sp.群落发生珊瑚白化,Pessir Selatan Regency经历了漂白。它发表在几家当地新闻上,成为地方和中央政府当局以及沿海社区的热门话题。关于造成这种现象的原因有很多不准确的地方。本研究旨在从海洋学的角度确定曼朱托海滩当地珊瑚白化的原因。使用TOA DKK水质检查器在Manjuto海滩周围收集的水质数据进行了描述性统计评估。本研究还利用DSASv5对海岸线的空间变化进行了分析。潮汐数据的时间序列也被用来分析潮差引起的盐度分层。还模拟了一个带有柔性网格的流动模型,以确定曼朱托海滩的水团运动和沿岸水流模式。与支持海洋生物的水质标准相比,溶解氧(DO)、温度和盐度显示出异常。在涨潮和退潮期间,其范围分别为5.8-11.2 mg/L、28-28.3℃和25-28℃。测量的其他参数(pH、电导率、浊度和密度)适用于海洋生物群。研究结果表明,潮差对盐度分层有着独特的影响。地下水供应的入侵导致盐度下降,导致曼朱托海滩当地珊瑚白化。盐度水平的变化也由0-0.31 m/s的潮流引发,导致累积盐度冲击。目前,曼朱托海滩正经历2.36-3.17米/年的吸积,通过洪水-退潮周期改变了水的覆盖范围。这些状态会导致累积的阳光照射和由洪水-潮汐周期引起的盐度冲击。这就是为什么当地珊瑚白化事件无疑是避免的。
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引用次数: 0
Evaluating Marine Debris Trends and the Potential of Incineration in the Context of the COVID-19 Pandemic in Southern Bali, Indonesia 在印度尼西亚巴厘岛南部2019冠状病毒病大流行背景下评估海洋垃圾趋势和焚烧潜力
Q4 Agricultural and Biological Sciences Pub Date : 2021-06-19 DOI: 10.20473/JIPK.V13I2.25164
W. K. Suryawan, A. Sarwono, I. Septiariva, Chun-Hung Lee
Highlight Research:The potential calorific value of marine debris obtained from calculating the total waste is 12.05 MJ/kg, which still falls within the incinerator application's standard criteria. AbstractMarine debris, a global environmental issue today, is a major threat to Bali’s seas which are famous for its natural beauty and aggravated by the spread of COVID-19 pandemic. This study aimed to determine the characteristics of marine debris in Bali Island, especially in the southern region and to analyze the feasibility of incineration as one of the waste treatment processes. This research was conducted by utilizing secondary data and literature reviews from related previous studies. Water content and caloric value were measured directly using the ASTM E 790-15 and ASTM D 5865-11a standards. Marine debris generation from 2013 to 2019 tends to decrease from 1.22 kg/km.day to 0.46 kg/km.day. Organic waste (59.4%) comprised the largest marine debris followed by plastic waste (13.4%) and diapers (11.9%). Thermal technology such as incineration can be introduced to treat marine debris. The standard application of incinerator technology is moisture content and caloric value. The water content of marine debris is reportedly 54.56%, therefore, further preliminary processing is needed, especially for waste with high moisture content, such as diapers and organic waste. The potential calorific value of marine debris during the COVID-19 pandemic obtained from calculating the total waste was 12.05 MJ/kg which still did not meet the incinerator application's standard criteria.
亮点研究:通过计算总废物得出的海洋废弃物的潜在热值为12.05 MJ/kg,仍在焚烧炉应用的标准范围内。摘要海洋废弃物是当今全球环境问题,是对巴厘岛海洋的主要威胁。巴厘岛以其自然美景而闻名,新冠肺炎疫情的蔓延加剧了这一威胁。本研究旨在确定巴厘岛,特别是南部地区海洋废弃物的特征,并分析焚烧作为废物处理工艺之一的可行性。这项研究是利用二次数据和文献综述从相关的先前研究进行的。使用ASTM E 790-15和ASTM D 5865-11a标准直接测量水含量和热值。从2013年到2019年,海洋废弃物的产生量往往从1.22公斤/公里/天减少到0.46公斤/公里-天。有机废物(59.4%)是最大的海洋废弃物,其次是塑料废物(13.4%)和尿布(11.9%)。可以引入焚烧等热技术来处理海洋废弃物。焚烧炉技术的标准应用是含水量和热值。据报道,海洋废弃物的含水量为54.56%,因此需要进一步的初步处理,尤其是对含水量高的废弃物,如尿布和有机废弃物。在新冠肺炎大流行期间,通过计算总废物获得的海洋废弃物的潜在热值为12.05 MJ/kg,仍不符合焚烧炉应用的标准标准。
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引用次数: 11
Molecular Expressions : Scanning Electron Microscope (SEM) in Gills of Cyprinus carpio Infected Myxobolus sp. 鲤鳃感染黏菌的分子表达:扫描电镜观察。
Q4 Agricultural and Biological Sciences Pub Date : 2021-06-19 DOI: 10.20473/JIPK.V13I2.25484
Maftuch Maftuch, Bramantiyo Satriyo Wicaksosno, Febi Nadhila Nurin, Andhang Sebastian
Highlight Research:It has been found that many fish died with wounds from farmers in Blitar, Indonesia.The mucosal smear test from mucosal gills of C. carpio infected with Myxobolus.In the blood smear test of C. carpio, there was no myxobolus found in the blood.In gill organ testing using SEM that Myxobolus is found in gills (C. carpio).The shape of Myxobolus resembles an imperfect ball with a hollow in the middle. AbstractThe biggest problem that is often considered to be an obstacle to Common carp culture is the emergence of disease attacks. One type of disease that often attacks the seeds of Cyprinus carpio is Myxobolus (a systemic parasite that can cause harm to fish farming). The aim of this study was to determine the molecular expression through the smear test on C. carpio gills, to determine the image of the gill organs of C. carpio using the SEM test, and to determine the description of the spores of Myxobolus sp. Data were analyzed using descriptive methods. Descriptive method used was comparative descriptive comparing molecular expression in the test of gill mucosal smear of fish using a light microscope and gill organ testing using Scanning Electron Microscope (SEM) on C. carpio infected by Myxobolus sp. In this study, the results showed that in C. carpio infected with true Myxobolus found the presence of Myxobolus in the mucosal smear test and SEM test on gill tissue, but not found in the blood smear test.
亮点研究:据发现,在印度尼西亚布利塔,许多鱼类因农民受伤而死亡。粘液泡菌感染的卡氏C.carpio粘膜鳃的粘膜涂片检查。在C.carpio的血液涂片检查中,血液中没有发现粘团。在使用SEM进行的鳃器官测试中,在鳃(C.carpio)中发现粘液泡。Myxobolus的形状类似于一个不完美的球,在中间有一个空心。通常被认为是鲤鱼养殖障碍的最大问题是疾病发作的出现。一种经常攻击鲤鱼种子的疾病是粘液泡病(一种会对鱼类养殖造成危害的系统性寄生虫)。本研究的目的是通过对C.carpio鳃的涂片测试来确定分子表达,使用SEM测试来确定C.carpi奥鳃器官的图像,并确定粘液菌孢子的描述。使用描述性方法分析数据。所用的描述方法是比较用光学显微镜对鱼的鳃粘膜涂片测试和用扫描电子显微镜(SEM)对粘液菌感染的C.carpio的鳃器官测试中的分子表达。在本研究中,结果表明,在感染真粘液泡的C.carpio中,在粘膜涂片检查和鳃组织SEM检查中发现粘液泡存在,但在血液涂片检查中没有发现。
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引用次数: 0
Gene Transmission, Growth, and Exogeneous Growth Hormone Expression of G2 Transgenic Betta Fish (Betta imbellis) G2转基因斗鱼(Betta imbellis)的基因传递、生长及外源生长激素表达
Q4 Agricultural and Biological Sciences Pub Date : 2021-06-19 DOI: 10.20473/JIPK.V13I2.25870
Nadia Ayuningthias, H. Nasrullah, Dinar Tri Soelistiyowati, Eni Kusrini, A. Alimuddin
Highlight ResearchThe F2 of GH-transgenic B. imbellis was successfully producedThe transgene inheritance by the F2 fish was more than 90%The growth and body size of transgenic fish was significantly higher than controlF2 fish reached a larger body size in a shorter period compared to the F1 AbstractIn our previous research, we had successfully produced G0 and G1 Pangasianodon hypophthalmus growth hormone (PhGH) transgenic B. imbellis, native ornamental betta from Indonesia, which its giant-sized variant has valuable price for the breeders. The G0 and G1 transgenic (TG) fish showed higher growth rate and body size compared to the non-transgenic (NT) fish. The study was aimed to produce and evaluate the consistencies of transgene transmission and expression in G2 generation. The growth rate and body size between TG and NT fish was also compared. The G2 generation was produced using crosses between TG and NT G1 fish: ♂TG × ♀TG, ♂TG × ♀NT, ♂NT × ♀TG, and ♂NT ×♀ NT. Fish were reared for 12 weeks, and transgene detection was performed using the polymerase chain reaction method (PCR) on isolated DNA from the caudal fin clips. The endogenous and exogenous GH expression analysis was conducted using the quantitative real-time PCR (qPCR) method. The results showed that the inheritance of the GH transgene by the G2 fish was more than 90% in all transgenic crosses. Endogenous GH was expressed at the same levels in the brain of TG and NT fish, but the exogenous GH expression was highly detected only in the TG fish. The G2 transgenic fish had a higher specific growth rate, up to 31%, compared to the control. The body length of TG crosses were 23−35% higher and had 111−135% higher body weight compared to NT fish. These results showed a promising approached in mass-producing stable lines of giant-sized betta using the GH-transgenic technology.
短句来源重点研究成功培育出了转gh基因的红鲌F2,其转基因遗传率达90%以上,转基因鱼的生长和体型均显著高于对照,F2鱼在较短的时间内达到了比F1更大的体型。摘要在我们前期的研究中,我们成功培育出了G0和G1型转PhGH基因的红鲌,这是印度尼西亚的一种原生观赏鱼。它的巨型变种对育种者来说价值不菲。G0和G1转基因(TG)鱼的生长速度和体大小均高于非转基因(NT)鱼。本研究旨在建立和评价转基因在G2代中传递和表达的一致性。并比较了TG鱼和NT鱼的生长速度和体型。用TG与NT G1鱼杂交产生G2代:♂TG ×♀TG、♂TG ×♀NT、♂NT ×♀TG和♂NT ×♀NT。饲养12周,用聚合酶链反应法(PCR)对尾鳍片段分离的DNA进行转基因检测。采用实时荧光定量PCR (quantitative real-time PCR, qPCR)方法进行内源和外源GH表达分析。结果表明,在所有转基因杂交中,G2鱼的GH基因遗传率均在90%以上。内源性GH在TG鱼和NT鱼的大脑中表达水平相同,但外源性GH只在TG鱼中高表达。与对照相比,G2转基因鱼的特定生长率更高,可达31%。TG杂交体长比NT高23 ~ 35%,体重比NT高111 ~ 135%。这些结果表明,利用gh转基因技术大批量生产稳定的巨型斗鱼系是一条有希望的途径。
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引用次数: 0
Retention and Survival Optimization of Juvenile Green Mussel (Perna Viridis) by Using Substrate from Seaweed Extract 海藻提取物基质对绿贻贝幼鱼的保留和存活优化
Q4 Agricultural and Biological Sciences Pub Date : 2021-06-19 DOI: 10.20473/JIPK.V13I2.23515
D. Sanjayasari, M. D. N. Meinita, Tjahjo Winanto
Highlight ResearchThe qualitative bioactive assay on terpenoid compound of six macroalgae species were tested.The effect of six macroalgae extracts as inducer mediating settlement to juvenile Perna viridis were observed.Three macroalgae species were potentially promote the retention of juvenile P. viridisAbstractThe low retention of juvenile of green mussels (Perna viridis) in the aquaculture holding system has become a constraint for its production. The depress number of juvenile mussel on the collector rope might be caused due to both limited spawning season and their secondary settlement behaviour. Therefore, providing suitable substrate which able to improve green mussel seed retention is required. One of the solutions is by applying inducer mediating settlement as substrate enrichment in order to optimize the retention of juvenile P. viridis. The potential substrates thought to have these inductive activities is seaweed. Seaweed bioactive compound which may improve juvenile mussel retention is terpenoid. Six seaweed extracts used in the current study and the terpenoid of these six macroalgae species were tested. Qualitatively all six seaweed showed a positive result on the terpenoid compound. The retention and survival of juvenile green mussel observed by using 20 conical tanks with a complete randomized design experiment. Each of the seaweed species tested separately comparing with three other experimental treatments under 24 h observation time, A (rope), B (rope + PhytagelTM), C (rope + PhytagelTM l+ solvent), D (rope + PhytagelTM + seaweed extract), it made four experimental treatments with 5 times replications. The result indicated a variation pattern on the retention of juvenile mussels according to the experimental substrate. The juvenile mussels were preferably settled on enriched substrate of G. latifolium and S. polycystum, extracts (p<0.05). Adding seaweed extracts on the substrate did not affect the mussels survival (p>0.05).
重点研究对6种大型藻类萜类化合物进行了定性生物活性测定。观察了6种大型藻提取物作为诱导剂对绿藻幼鱼沉降的影响。摘要绿贻贝(Perna viridis)在养殖饲养系统中幼鱼滞留率低已成为制约其生产的一个因素。收集绳上贻贝幼鱼数量的减少可能是由于产卵季节有限和贻贝的二次沉降行为所致。因此,需要提供合适的基质来提高青贻贝种子的保留率。其中一种解决方案是利用诱导剂介导沉降作为底物富集,以优化绿假单胞藻幼鱼的保留率。被认为具有这些诱导活性的潜在底物是海藻。可以提高幼贻贝保留率的海藻生物活性化合物是萜类化合物。本研究对六种海藻提取物及其萜类化合物进行了检测。6种海藻的萜类化合物定性检测结果均为阳性。采用20个完全随机设计试验,对青贻贝幼鱼的滞留和存活进行了观察。每种海藻在24 h的观察时间内分别与A(绳子)、B(绳子+ PhytagelTM)、C(绳子+ PhytagelTM l+溶剂)、D(绳子+ PhytagelTM +海藻提取物)3个处理进行比较,共4个处理,重复5次。结果表明,不同基质对贻贝幼鱼的滞留率有一定的影响。幼贻贝最好生长在阔叶草和多囊草提取物的富集基质上(p0.05)。
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引用次数: 0
Evaluation of Carboxymethyl Cellulose (CMC) Binder and Squid Oil Attractant in the Formulation of Litopenaeus vannamei Diet 羧甲基纤维素(CMC)粘合剂和鱿鱼油引诱剂在凡纳滨对虾日粮配方中的评价
Q4 Agricultural and Biological Sciences Pub Date : 2021-06-19 DOI: 10.20473/JIPK.V13I2.24428
N. Yusoff, M. I. Zakariah, Jalil Mohamad, Sharifah Rahmah, Marina Hassan
Highlight ResearchEffects of carboxymethyl cellulose (CMC) binder on pellet water stability in shrimp diet.Evaluation of squid oil attractant on feed palatability.The quality of water after inclusion of CMC binder and squid oil in pellet.CMC and squid oil improved dry matter retention and feeding responses in shrimp. AbstractPellet water stability and feeding attractant are the crucial factors to be considered in the formulation of shrimp feed to minimize nutrient leaching and improve food palatability, respectively. The aim of this study was to determine the binding effect of supplementation of carboxymethyl cellulose (CMC) during pellet manufacturing, and feeding responses with the inclusion of squid oil in pellet. Both experiments were conducted separately in five feed formulations ranged from 0.00% to 3.00% of CMC and squid oil. All T0 was referred as control with no CMC or squid oil. Experiment was conducted using 30 shrimps/treatment with triplicates. The dry matter retention up to 120 min of immersion and the feeding response test were evaluated in pellet supplemented with CMC and squid oil, respectively. Results showed the percentage of dry matter retentions increased with the increase of CMC, over time. The CMC containing pellets at 2.00% and 3.00% had minimal disintegration in water and possessed high water stability after 120 min immersion. The addition of 3.00% squid oil in feed exhibited a significant result (p<0.05) in the time taken for shrimp to access the pellet, also increased the time starts for ingestion. No significant change was observed in water quality indicated no water contamination occurred throughout the study. Results demonstrated that the addition of CMC binder at 2.00% and 3.00% improved pellet water stability, whilst 3.00% squid oil was recommended to enhance feed palatability. However, future studies on the activity of enzymes in shrimp bodies after treatment would be an advantage.
羧甲基纤维素(CMC)粘结剂对虾饲料中颗粒水稳定性的影响。鱿鱼油引诱剂对饲料适口性的影响。对CMC粘结剂与鱿鱼油包合后的水质进行了研究。CMC和鱿鱼油改善了虾的干物质保留和摄食反应。摘要颗粒水稳定性和饵料引诱剂分别是对虾饲料配方中减少养分淋失和提高食品适口性的关键因素。本研究的目的是确定在颗粒制造过程中添加羧甲基纤维素(CMC)的结合效果,以及在颗粒中加入鱿鱼油的摄食反应。两项试验分别在0.00% ~ 3.00% CMC和鱿鱼油的5种饲料配方中进行。所有T0均为对照组,不加CMC或鱿鱼油。试验选用30只对虾/处理,设3个重复。分别对添加CMC和鱿鱼油的颗粒进行浸泡至120 min时的干物质保留率和摄食反应试验。结果表明,随着时间的推移,干物质保留率随CMC浓度的增加而增加。含2.00%和3.00%球团的CMC在水中崩解最小,浸泡120 min后具有较高的水稳定性。饲料中添加3.00%鱿鱼油显著影响了对虾接近颗粒的时间(p<0.05),并延长了对虾开始摄食的时间。在整个研究过程中,没有观察到水质的显著变化,表明没有发生水污染。结果表明,添加2.00%和3.00% CMC粘结剂可提高颗粒的水稳定性,添加3.00%鱿鱼油可提高饲料的适口性。然而,对处理后虾体酶活性的进一步研究将是一个优势。
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引用次数: 1
The Effectiveness of Filopaludina javanica and Sulcospira testudinaria in Reducing Organic Matter in Catfish (Clarias sp.) Aquaculture Wastewater 爪哇丝藻和毛螺藻对鲶鱼养殖废水中有机物的还原效果研究
Q4 Agricultural and Biological Sciences Pub Date : 2021-03-31 DOI: 10.20473/JIPK.V13I1.23085
S. Lailiyah, D. Arfiati, A. Hertika, Neni Diah Kusumaning Arum, Catur Budi Noviya
HighlightCatfish aquaculture wastewater contains organic matter that exceeds the quality standard of 68.75 mg / L and 72.48 mg / L.Tutut Jawa snail (F. javanica) can reduce organic matter in waste by up to 92%.Susuh Kura snail (S. testudinaria) can reduce organic matter in waste by up to 87%.Catfish aquaculture wastewater treatment with snails only takes 8 hours. Abstract The residual or wastewater from the cultivation process that is discharged directly into common waters can reduce the quality of the waters because it contains a lot of organic material from organism feces and feed residue, so it needs management first. The purpose of this study was to determine the ability of Javanese snails and freshwater gastropods to reduce total organic matter in catfish culture waste. The research method used was the experiment with 2 factor Factorial completely randomized design, namely the measurement time (4, 8, 12, and 16 hours) and the abundance of the Tutut Java snail and freshwater gastropods (100%, 75%, 50%, 25%, and control) with 5 repetitions. Tutut Java snails were taken from the pond at UPR Sumbermina Dau and brought to the laboratory. The results obtained in the treatment with the addition of  Tutut Java snail, the total organic matter content decreased at 75% snail density by 90% (from 68.75 mg / L to 5.31 mg / L) at the 12th hour. The treatment with the addition of Freshwater Gastropods at a density of 25%, the total organic matter content decreased by 87% (from 72.48 to 9.35 mg / L) at the 8th hour. Water quality during the study was in a condition that could tolerate the Tutut Java snails and freshwater gastropods. The conclusion is that the addition of Tutut Java snail and / or freshwater gastropods can reduce the total organic matter content to the water quality standard.
鲶鱼养殖废水中有机物含量分别超过68.75 mg / L和72.48 mg / L的质量标准,tutut爪哇螺(F. javanica)可减少废水中有机物含量高达92%。Susuh Kura蜗牛(S. testudinaria)可以减少废物中高达87%的有机物。用螺处理鲶鱼养殖废水只需8小时。摘要养殖过程中产生的残渣或废水由于含有大量的生物粪便和饲料残渣中的有机物,直接排放到普通水体中,会降低水体的水质,因此首先需要对其进行管理。本研究的目的是确定爪哇蜗牛和淡水腹足类动物减少鲶鱼养殖废弃物中总有机质的能力。研究方法采用2因素全随机试验设计,即测定时间(4、8、12、16 h)和Tutut爪哇蜗牛和淡水腹足动物的丰度(100%、75%、50%、25%和对照),共5次重复。从普遍定期审议Sumbermina Dau的池塘中取出Tutut爪哇蜗牛并带到实验室。结果表明,添加Tutut爪哇岛螺后,在75%螺密度下,处理12 h时,总有机质含量从68.75 mg / L下降到5.31 mg / L,下降了90%。添加25%密度的淡水腹足类处理,8 h时总有机质含量由72.48 mg / L下降至9.35 mg / L,降幅达87%。在研究期间,水质处于可以容忍图坦卡蒙爪哇蜗牛和淡水腹足动物的条件下。综上所述,添加Tutut Java蜗牛和/或淡水腹足类动物可使总有机质含量降低至水质标准。
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引用次数: 3
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Jurnal Ilmiah Perikanan dan Kelautan
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