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Integration of Tetraselmis chuii and Artemia sp. Culture in Industrial-Scale Salt Production 工业规模制盐中的四膜虫和蒿属藻类综合养殖
Q4 Agricultural and Biological Sciences Pub Date : 2024-01-27 DOI: 10.20473/jipk.v16i1.50514
M. A. Alamsjah, Himnna Sayyidatul Islamiyah, Ghishella Ayu Rahmawati, Fadhilah Atika Putri, Rizka Sandra Amalia, Putranti Hikmah Triningtyas, Dwi Yuli Pujiastuti, Adibi Rahiman bin Md Nor
AbstractThe common technique of traditional salt production in Indonesia is seawater evaporation technology, which uses sunlight to produce salt crystals from seawater. However, in general, the applied technology only produces salt with about 80% NaCl and water contents >7%, impurities >2%, and other contaminants. This produced salt is not suitable to be used as industrial salt. This study aimed to determine the effects of liquid organic fertilizer Gracilaria sp. addition into the culture media of Tetraselmis chuii on Artemia sp. growth, to identify the profile of length, weight, and survival rate of Artemia sp., and finally attempted to uncover the contribution of Artemia sp. in impurity mineral absorption and NaCl content improvement. The results of the study showed that the density of T. chuii based on the concentration of liquid organic fertilizer Gracilaria sp. was significantly different (p<0.05) in each treatment. T. chuii cultivation using liquid organic fertilizer Gracilaria sp. 16 mg N/mL resulted in the highest density on the culture days. However, the studies showed that there was no significant effect (p>0.05) on the weight and length gain of Artemia sp. The research conducted can support the downstream use of products from engineering research in the field of technology to increase the added value of salt products and be part of the contribution to the achievement of the 14th SDG on life below water.Highlight ResearchIntegration of chuii and Artemia sp. culture in salt production could improve NaCl contents by up to 97% and reduced impurities of sulfate and magnesium. The result of the first phase of this study is the density of chuii. This finding is useful to find out the effects of Gracilaria sp. liquid organic fertilizer administration as a source of nitrogen that improves the growth of T. chuii.The highest density of T. chuii resulted from a treatment receiving the dose of 16 N mg/L Gracilaria sp. liquid organic fertilizer with > 5 x 106 cells/mL. This finding indicates that the administration of 16 N mg/L Gracilaria liquid organic fertilizer into the culture medium provided a source of nitrogen and phosphorus for the metabolism of T. chuii.The results of the mineral content test on Artemia sp. show the largest percentage change in Mg2+ > SO42- > Cl- > Na+. The densities of Artemia sp. showed a positive trend of increasing levels of Mg2+ and SO42-, which coincided with decreasing levels of Mg2+ and SO42- in seawater media.The results of NaCl content measurement in water with Artemia sp. culture integration show salt with excellent quality of 97% that met the criteria of industrial salt. The contribution of Artemia sp. to improving the quality of salt products is interesting and has the potential to serve as an alternative to the traditional salt-farming system that is currently practiced.
摘要 印度尼西亚传统制盐的常用技术是海水蒸发技术,即利用阳光从海水中生产盐晶体。然而,一般来说,这种应用技术只能生产出氯化钠含量约为 80%、含水量大于 7%、杂质含量大于 2% 以及其他污染物的盐。这种盐不适合用作工业盐。本研究旨在确定在四大家鱼培养基中添加液态有机肥 Gracilaria sp.对竹节虫生长的影响,确定竹节虫的体长、体重和存活率,并试图揭示竹节虫在吸收杂质矿物和提高 NaCl 含量方面的贡献。研究结果表明,基于液体有机肥 Gracilaria sp.浓度的 T. chuii 密度对 Artemia sp.增重和增长有显著差异(P0.05),而基于液体有机肥 Gracilaria sp.浓度的 T. chuii 密度对 Artemia sp.增重和增长有显著差异(P0.05)。本研究可支持工程研究产品在技术领域的下游使用,以提高盐产品的附加值,并为实现关于水下生活的第 14 个可持续发展目标做出贡献。本研究第一阶段的结果是鳜鱼的密度。这一发现有助于了解施用 Gracilaria sp.液体有机肥作为氮源对改善 T. chuii 生长的影响。这一结果表明,在培养基中施用 16 N mg/L Gracilaria 液体有机肥为 T. chuii 的新陈代谢提供了氮和磷的来源。对 Artemia sp.进行的矿物质含量测试结果显示,Mg2+ > SO42- > Cl- > Na+ 的变化百分比最大。海带中 NaCl 含量的测量结果显示,盐的质量非常好,达到 97%,符合工业盐的标准。藻类对提高盐产品质量的贡献很有意义,有可能成为目前传统盐业系统的替代品。
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引用次数: 0
Social Structure Analysis in Management of South Sumatra Inland Waters 南苏门答腊内陆水域管理中的社会结构分析
Q4 Agricultural and Biological Sciences Pub Date : 2024-01-27 DOI: 10.20473/jipk.v16i1.48081
Fevi Rahma Dwi Putri, Arif Satria, R. Kinseng, Taryono Taryono
AbstractThe social structure of inland fisheries communities tends to place powerful elites in important positions, while local fishermen are subordinate actors. Patronage is a form of social relationship that describes the unequal social structure between these actors. This research aimed to analyze social structure in the inland waters of South Sumatra in the practice of the Lebak Lebung and River Auction (L3S) in OKI District. To achieve this goal, a constructivist paradigm with qualitative research methods was employed, and the sample comprised 39 informants selected as subjects. The data analysis was conducted in three distinct stages, namely data reduction, presentation, and drawing conclusions. The results showed that the regulation of open access for all parties in the local government regime after 1982 created an institutionalized social relations structure where the government, investors, traders, middlemen, and non-fishing actors dominated compared to the fishermen. The participants in this social landscape strived to accumulate capital to enhance their competitiveness within the L3S domain. A patronage system was implemented with middlemen assuming the role of patron and the fishermen acting as their clients. The current institutional social structure has created an unfair distribution of resource benefits for fishing workers. The novelty theories were found in the working relations of the inland waters community of South Sumatra, namely stratified distribution of rights, dual status, multi-stage patronage, and the positive function of patronage for chief fisherman. Further research should be conducted to research policy revitalization and develop strategic inland water management models.Highlight ResearchThe author mentioned 3 highlights from their results research:Auction system in inland waters in South Sumatra presents an unfair distribution for fishermen.Structure of the working relationship formed is an asymmetric patron-client relationship but with novel theories such as the stratified distribution of rights model, dual status, multi-stage patronage, and the positive function of patronage.An imbalance in local access to auction objects due to their weak ability to accumulate capital.
摘要 内陆渔业社区的社会结构往往是有权势的精英占据重要位置,而当地渔民则处于从属地位。赞助是一种社会关系形式,描述了这些行为者之间不平等的社会结构。本研究旨在分析南苏门答腊内陆水域在 OKI 区 Lebak Lebung 和河流拍卖(L3S)实践中的社会结构。为实现这一目标,本研究采用了建构主义范式和定性研究方法,并选取了 39 名信息提供者作为研究对象。数据分析分三个不同阶段进行,即数据还原、展示和得出结论。结果表明,1982 年后地方政府对各方开放准入的规定形成了一种制度化的社会关系结构,与渔民相比,政府、投资者、贸易商、中间商和非渔业参与者占据主导地位。在这一社会格局中,参与者努力积累资本,以提高他们在 L3S 领域的竞争力。中间商充当主顾,渔民则是他们的客户。当前的制度性社会结构造成了渔业工人资源利益分配不公。在南苏门答腊内陆水域社区的工作关系中发现了一些新颖的理论,即权利的分层分配、双重地位、多级庇护以及庇护对首席渔民的积极作用。作者在研究成果中提到了 3 个亮点:南苏门答腊内陆水域的拍卖制度对渔民来说是一种不公平的分配制度;形成的工作关系结构是一种不对称的主顾-客户关系,但具有新颖的理论,如权利的分层分配模式、双重地位、多阶段主顾和主顾的积极功能;由于当地人积累资金的能力较弱,他们在获得拍卖对象方面存在不平衡。
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引用次数: 0
Sustainable Pangasius Aquaculture Management Strategy using Multidimensional Scaling (MDS) and Analytical Hierarchy Process (AHP) in Tulungagung Regency, East Java, Indonesia 在印度尼西亚东爪哇 Tulungagung 地区使用多维尺度 (MDS) 和层次分析法 (AHP) 制定可持续的鱼芒养殖管理策略
Q4 Agricultural and Biological Sciences Pub Date : 2024-01-03 DOI: 10.20473/jipk.v16i1.49377
Asro Nurhabib, Aida Sartimbul, M. Primyastanto, M. Widodo, Lugu Tri Handoko, Andra Rejekining Rahayu, Suharun Martudi
AbstractAquaculture activities in Tulungagung Regency provide livelihoods for 12,050 households. Therefore, ensuring the sustainability of Pangasius aquaculture activities is crucial for the welfare of fish farmers. This study aims to analyze the sustainability of existing Pangasius aquaculture businesses in Tulungagung Regency, East Java, Indonesia, and to create a sustainable Pangasius aquaculture policy strategy. This quantitative study used Multi-Dimensional Scaling (MDS) and Analytical Hierarchy Process (AHP) analyses to determine the sustainability status of Pangasius aquaculture based on five dimensions: ecological, economic, social, institutional, and technological infrastructure. Among these dimensions, two are less sustainable, particularly infrastructure technology and the economy.Highlight ResearchThe author mentioned four highlights from their research:Tulungagung Regency is East Java Province's major pangasius producer.MDS can assess sustainability across multiple dimensions.AHP enables the prioritization of strategies that must be implemented.The Pangasius aquaculture company in Tulungagung Regency is currently in a sustainable state.
摘要 图伦加贡地区的水产养殖活动为 12,050 户家庭提供了生计。因此,确保鱼芒养殖活动的可持续发展对养鱼户的福利至关重要。本研究旨在分析印度尼西亚东爪哇图伦加贡县现有鱼芒养殖业的可持续性,并制定可持续鱼芒养殖政策战略。这项定量研究采用了多维尺度(MDS)和层次分析法(AHP)分析方法,从生态、经济、社会、制度和技术基础设施五个方面确定了鱼芒养殖的可持续性状况。在这些维度中,有两个维度的可持续性较差,尤其是基础设施技术和经济。研究亮点作者提到了他们研究的四个亮点:图伦加贡地区是东爪哇省的主要鱼芒生产地。
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引用次数: 0
Feminization of Maroon Clownfish (Amphiprion biaculeatus, Bloch 1790) with 17β-Estradiol Hormonal Induction 17β-雌二醇激素诱导的褐皮小丑鱼(Amphitrion biaculeatus,Bloch 1790)雌化
Q4 Agricultural and Biological Sciences Pub Date : 2023-08-05 DOI: 10.20473/jipk.v15i2.42765
H. Sahusilawane, A. Sudrajat, M. A. Suprayudi, D. Soelistyowati, L. Tumbelaka, I. Effendi
Highlight ResearchThe 17β-estradiol hormone induction reduces the percentage of R values in skin and fin color by as much as 50-60%.The 17β-estradiol hormone induction increases total length and body weight, estradiol content, GIS and HIS, and also glucose content.The 17β-estradiol hormone induction shows mature development of the gonadal profile from functional male to functional female.Abstract As maroon clownfish (Amphiprion biaculeatus) is a protandrous hermaphroditic fish, feminization process with 17β-estradiol hormone can be applied to accelerate the female broodstock candidate supply for further spawning effort. This study aimed to evaluate the feminization of A. biaculeatus with 17β-estradiol hormonal induction. This study used a completely randomized design with several hormone dosage, namely P0 (without 17β-estradiol hormone induction), P1 (0.5 μg 17β-estradiol/g body), P2 (1.0 μg 17β-estradiol/g body), and P3 (1.5 μg 17β-estradiol/g body). These treatments were applied with three replications. Five fish composed of α-fish, β-fish, and three γ-fish were reared in each aquarium for 90 days with a flowing water system. The α- and β-fish were then removed, while the γ-fish was injected with hormone. Otohime pellet feed was fed three times a day until apparent satiation. The results showed that the 17β-estradiol hormone could induce 100% of the feminization process of male A. biaculeatus. The dosage of P3 obtained the lowest value percentage of red, green, blue (RGB), but showing the highest total of length and body weight (6.67±0.42 cm and 6.40±0.78 g, respectively), estradiol content (149.73±4.24 ρg/mL), GSI and HSI (0.38±0.07% and 3.59±0.49%), and glucose content (4.67±0.64 mg/dL), followed by more mature gonad profile than other treatments. This condition indicates that fish in P3 treatment has been reversed as functional female. The average survival rate for the treatment was as high as 60%. Therefore, the application of 17β-estradiol hormonal induction is effective for the feminization process in A. biaculeatus as a protandrous hermaphroditic fish.
重点研究17β-雌二醇激素的诱导可使皮肤和鳍颜色中R值的百分比降低50-60%。17β-雌二醇激素的诱导增加了总长度和体重、雌二醇含量、GIS和HIS以及葡萄糖含量。17β-雌二醇激素的诱导显示性腺从功能性雄性向功能性雌性的成熟发育。摘要由于栗色小丑鱼是一种雌雄同体的前体鱼类,因此可以应用17β-雌二醇的雌性化过程来加速雌性候选种群的供应,以进一步产卵。本研究旨在评估17β-雌二醇激素诱导下双库勒线虫雌性化的情况。本研究采用了一种完全随机的设计,有几种激素剂量,即P0(无17β-雌二醇激素诱导)、P1(0.5μg 17β-雌激素/g身体)、P2(1.0μg 17α-雌二醇/g身体)和P3(1.5μg 17γ-雌二醇/克身体)。对这些处理进行了三次重复。在流动的水系统中,每个水族馆饲养5条鱼,包括α鱼、β鱼和3条鱼,为期90天。然后取出α-和β-鱼,同时给γ-鱼注射激素。Otohime颗粒饲料每天喂三次,直到明显饱腹。结果表明,17β-雌二醇能诱导雄性双钩线虫100%的雌化过程。P3剂量的红、绿、蓝(RGB)值百分比最低,但总长度和体重最高(分别为6.67±0.42cm和6.40±0.78g),雌二醇含量(149.73±4.24ρg/mL),GSI和HSI(0.38±0.07%和3.59±0.49%),葡萄糖含量(4.67±0.64mg/dL),其次是比其他处理更成熟的性腺。这种情况表明,P3处理中的鱼已被逆转为功能性雌性。该治疗的平均存活率高达60%。因此,应用17β-雌二醇激素诱导对作为雄性雌雄同体鱼类的双壳A.biaculeatus的雌性化过程是有效的。
{"title":"Feminization of Maroon Clownfish (Amphiprion biaculeatus, Bloch 1790) with 17β-Estradiol Hormonal Induction","authors":"H. Sahusilawane, A. Sudrajat, M. A. Suprayudi, D. Soelistyowati, L. Tumbelaka, I. Effendi","doi":"10.20473/jipk.v15i2.42765","DOIUrl":"https://doi.org/10.20473/jipk.v15i2.42765","url":null,"abstract":"Highlight Research\u0000\u0000The 17β-estradiol hormone induction reduces the percentage of R values in skin and fin color by as much as 50-60%.\u0000The 17β-estradiol hormone induction increases total length and body weight, estradiol content, GIS and HIS, and also glucose content.\u0000The 17β-estradiol hormone induction shows mature development of the gonadal profile from functional male to functional female.\u0000\u0000Abstract\u0000 \u0000As maroon clownfish (Amphiprion biaculeatus) is a protandrous hermaphroditic fish, feminization process with 17β-estradiol hormone can be applied to accelerate the female broodstock candidate supply for further spawning effort. This study aimed to evaluate the feminization of A. biaculeatus with 17β-estradiol hormonal induction. This study used a completely randomized design with several hormone dosage, namely P0 (without 17β-estradiol hormone induction), P1 (0.5 μg 17β-estradiol/g body), P2 (1.0 μg 17β-estradiol/g body), and P3 (1.5 μg 17β-estradiol/g body). These treatments were applied with three replications. Five fish composed of α-fish, β-fish, and three γ-fish were reared in each aquarium for 90 days with a flowing water system. The α- and β-fish were then removed, while the γ-fish was injected with hormone. Otohime pellet feed was fed three times a day until apparent satiation. The results showed that the 17β-estradiol hormone could induce 100% of the feminization process of male A. biaculeatus. The dosage of P3 obtained the lowest value percentage of red, green, blue (RGB), but showing the highest total of length and body weight (6.67±0.42 cm and 6.40±0.78 g, respectively), estradiol content (149.73±4.24 ρg/mL), GSI and HSI (0.38±0.07% and 3.59±0.49%), and glucose content (4.67±0.64 mg/dL), followed by more mature gonad profile than other treatments. This condition indicates that fish in P3 treatment has been reversed as functional female. The average survival rate for the treatment was as high as 60%. Therefore, the application of 17β-estradiol hormonal induction is effective for the feminization process in A. biaculeatus as a protandrous hermaphroditic fish.","PeriodicalId":17760,"journal":{"name":"Jurnal Ilmiah Perikanan dan Kelautan","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43582564","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Microplastic Distribution in Beach Sediments: Comparison Between the North and South Waters of East Java Island, Indonesia 微塑料在海滩沉积物中的分布:印度尼西亚东爪哇岛南北水域的比较
Q4 Agricultural and Biological Sciences Pub Date : 2023-08-04 DOI: 10.20473/jipk.v15i2.41065
D. Yona, F. Setyawan, Sisylia Eka Narriyah Putri, F. Iranawati, Muhammad Ariq Kautsar, A. Isobe
Highlight ResearchThis is the first study of microplastics in four beach sediments in the east part of Java Island.Bahak and Pesona Beach are connected to Java Sea (north part), while Tambak Rejo and Balekambang Beach are connected to Indian Ocean (south part).A higher microplastics were observed on the beaches of the north part compared to the south part.Local sources and oceanographic parameters of the beaches influence the presence of microplastics in the study areas.AbstractMicroplastic pollution in beach sediments has been studied intensively worldwide, but there are limited studies in the beach areas of the eastern Java Island, Indonesia. This study aimed to identify the distribution of microplastic in four beaches in Indonesia: Bahak, Pesona, Tambakrejo, and Balekambang. The first two beaches are located in the north of Java Island and influenced by the east Java Sea, while the last two are located in the south and influenced by the Indian Ocean. Sediment samples were collected along the strandline inside the 1 × 1 m transect quadrate in the top 5 cm using a stainless-steel shovel. Microplastic and granulometry analyses were conducted to obtain microplastic and sediment grain size data, respectively. Physical parameters of the beaches, such as wind, wave, and ocean current, were calculated using Copernicus and NASA (PODAAC), respectively. The total abundance of microplastic ranged from 54.7 ± 48.6 to 103.3 ± 4.7 particles kg-1 with the following descending order: Bahak > Pesona > Tambakrejo > Balekambang. Although there was no statistically significant difference in microplastic concentrations among the beaches, the beaches connected to the Java Sea accumulated more microplastics than the ones connected to the Indian Ocean. Fiber and blue were the dominating type and colors of microplastic. The results confirm that the distribution of microplastic is associated with the morphology of the beaches and the local source.
亮点研究这是首次对爪哇岛东部四个海滩沉积物中的微塑料进行研究。Bahak和Pesona海滩与爪哇海(北部)相连,而Tambak Rejo和Balekambang海滩与印度洋(南部)相连。与南部相比,北部海滩的微塑料含量更高。海滩的当地来源和海洋学参数影响研究区域内微塑料的存在。摘要世界各地对海滩沉积物中的微塑料污染进行了深入的研究,但对印度尼西亚爪哇岛东部海滩地区的研究有限。本研究旨在确定印度尼西亚四个海滩的微塑料分布:巴哈克、佩索纳、坦巴克雷霍和巴列坎邦。前两个海滩位于爪哇岛北部,受东爪哇海影响,后两个位于南部,受印度洋影响。使用不锈钢铲沿顶部5cm的1×1m样带象限内的绞线采集沉积物样本。分别进行微塑料和粒度分析以获得微塑料和沉积物粒度数据。海滩的物理参数,如风、波浪和洋流,分别使用哥白尼和美国国家航空航天局(PODAAC)计算。微塑料的总丰度范围为54.7±48.6至103.3±4.7个颗粒kg-1,按以下降序排列:Bahak>Pesona>Tambakrejo>Balekambang。尽管海滩之间的微塑料浓度没有统计学上的显著差异,但与爪哇海相连的海滩比与印度洋相连的海滩积累了更多的微塑料。纤维和蓝色是微塑料的主要类型和颜色。结果证实,微塑料的分布与海滩的形态和局部来源有关。
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引用次数: 0
Black Soldier Fly (Hermetia illucens) Oil Inclusion and its Effects on Growth Performances in Common Carp (Cyprinus carpio) 黑兵蝇油包体及其对鲤鱼生长性能的影响
Q4 Agricultural and Biological Sciences Pub Date : 2023-07-20 DOI: 10.20473/jipk.v15i2.39846
V. Herawati, Heni Tri Jayanti, T. Elfitasari, S. Putro, S. Windarto
Highlight ResearchUse of maggot oil as an addition to feed can increase the level of feed consumption of carp.The addition of maggot oil ( illucens) had a significant effect (P<0.05) on TFC, FCR, FUE, SGR, and survival of common carp.The use of maggot oil can be an alternative to using feed that is more effective and economical. AbstractThe demand for common carp (Cyprinus carpio) either its seed or consumption measures has increased every year. Fish growth can be increased by adding nutrients in the form of animal oil sources. An alternative ingredient that can be used to support fish growth with good nutritional content and relatively inexpensive is maggot oil or black soldier fly (H. illucens) oil. The purpose of this study was to determine the effect of maggot oil (H. illucens) in artificial feed on the growth performance of common carp and to determine the best dose of maggot oil. This study used a complete randomized design (CRD) with four treatments and three replicates: A, B, C, and D with the addition of maggot oil doses of 0%, 10%, 15%, and 20%, respectively. The test fish used was a common carp fry measuring 3.59±0.06 cm, 0.79±0.05 g. Observation time was 30 days with a stocking density of 15 fish/aquarium. The results showed that the addition of maggot oil (H. illucens) had a significant effect (P<0.05) on TFC, FCR, FUE, SGR, and survival. Based on the results of the study, the best dose was obtained in the addition of 15% maggot oil with value of TFC 10.57±0.26 g, FCR 1.52±0.03, FUE 65.43±1.54%, SGR 1.57±0.04%/day, and SR 95.56±3.85%. For further research, it is possible to use magot oil to increase carp production.
在饲料中添加蛆油可以提高鲤鱼的饲料消耗水平。添加蛆油对鲤鱼TFC、FCR、FUE、SGR和成活率有显著影响(P<0.05)。使用蛆油可以替代使用饲料,更有效和经济。摘要鲤鱼(Cyprinus carpio)的种子和消费需求逐年增加。可以通过添加动物油来源形式的营养物质来促进鱼的生长。另一种可用于支持鱼类生长的成分是蛆油或黑蝇油,它的营养成分好,价格相对便宜。本试验旨在研究人工饲料中蛆油对鲤鱼生长性能的影响,并确定蛆油的最佳投加量。采用完全随机设计(CRD), a、B、C、D 4个处理,3个重复,蛆油添加量分别为0%、10%、15%和20%。试验鱼为普通鲤鱼鱼苗,体长3.59±0.06 cm, 0.79±0.05 g。观察时间为30 d,放养密度为15尾/缸。结果表明,添加蛆油对TFC、FCR、FUE、SGR和成活率均有显著影响(P<0.05)。结果表明,添加15%蛆虫油为最佳剂量,TFC为10.57±0.26 g, FCR为1.52±0.03,FUE为65.43±1.54%,SGR为1.57±0.04%/d, SR为95.56±3.85%。在进一步的研究中,可以使用magot油来增加鲤鱼的产量。
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引用次数: 0
Effects of Various Nitrogen Sources on the Growth and Biochemical Composition of Chlorella sp. 不同氮源对小球藻生长及生化组成的影响。
Q4 Agricultural and Biological Sciences Pub Date : 2023-07-20 DOI: 10.20473/jipk.v15i2.43182
A. Yuniarti, M. Fakhri, N. Arifin, A. M. Hariati
Highlight ResearchEach species of microalga has a preferable nitrogen source for their optimal growth.The nitrogen sources in the grown media affected the growth rate and biochemical composition of Chlorella FNUB01.(NH2)2CO (urea) was found to be the best alternative nitrogen source for Chlorella FNUB01.For producing 1 g of Chlorella FNUB01, the use of urea reduced the cost of medium by 72.6%.AbstractChlorella sp. is a potential microalgae species to be produced commercially for feed, growth accelerator, and immuno-modulator in fish and shrimp culture. This study aimed to evaluate the various nitrogen sources on the growth, biomass production, and biochemical composition of Chlorella sp. FNUB01. The nitrogen sources used in this study were urea (NH2)2CO, potassium nitrate (KNO3), and ammonium nitrate (NH4NO3). Sodium nitrate (NaNO3) was used as a control as it is a part of the commercial medium BG-11. Generally, the sources of nitrogen in the media affected the growth and chemical composition of Chlorella sp. FNUB01. This green microalga grew better in the urea-containing medium which accounted for 1.5 times the concentration of that cultured in BG-11 (40 x106 cells. mL-1). Meanwhile, this microalgae species experienced the lowest growth when cultured in NH4NO3-containing medium. The biomass productivity of Chlorella sp. FNUB01 cultured in urea (0.93 g.L-1) was comparable to those grown with NaNO3 as the N source. A similar pattern was recorded for protein, chlorophyll, and carotenoid content as these biochemical contents were affected by N availability in the medium. Urea was an alternative low-cost N source for the culture of Chlorella sp. FNUB01. Replacement of NaNO3 with urea could reduce the cost of the medium by 72.6%.
亮点研究每种微藻都有一个优选的氮源,以实现最佳生长。生长培养基中的氮源影响小球藻FNUB01的生长速率和生化成分。(NH2)2CO(尿素)是小球藻FNUB01的最佳替代氮源。对于生产1g小球藻的FNUB01,使用尿素可使培养基成本降低72.6%,生长促进剂和免疫调节剂。本研究旨在评估不同氮源对小球藻FNUB01生长、生物量生产和生化组成的影响。本研究中使用的氮源为尿素(NH2)2CO、硝酸钾(KNO3)和硝酸铵(NH4NO3)。硝酸钠(NaNO3)用作对照,因为它是商业培养基BG-11的一部分。一般来说,培养基中的氮源影响小球藻的生长和化学成分。FNUB01。这种绿色微藻在含尿素的培养基中生长得更好,该培养基的浓度是在BG-11中培养的浓度的1.5倍(40×106个细胞/mL-1)。同时,当在含有NH4NO3的培养基中培养时,该微藻物种经历了最低的生长。在尿素(0.93g.L-1)中培养的小球藻FNUB01的生物量生产力与以NaNO3为氮源生长的小球菌相当。蛋白质、叶绿素和类胡萝卜素含量也记录了类似的模式,因为这些生物化学含量受到培养基中氮有效性的影响。尿素是小球藻FNUB01培养的替代低成本氮源。用尿素代替NaNO3可以使培养基的成本降低72.6%。
{"title":"Effects of Various Nitrogen Sources on the Growth and Biochemical Composition of Chlorella sp.","authors":"A. Yuniarti, M. Fakhri, N. Arifin, A. M. Hariati","doi":"10.20473/jipk.v15i2.43182","DOIUrl":"https://doi.org/10.20473/jipk.v15i2.43182","url":null,"abstract":"Highlight Research\u0000\u0000Each species of microalga has a preferable nitrogen source for their optimal growth.\u0000The nitrogen sources in the grown media affected the growth rate and biochemical composition of Chlorella FNUB01.\u0000(NH2)2CO (urea) was found to be the best alternative nitrogen source for Chlorella FNUB01.\u0000For producing 1 g of Chlorella FNUB01, the use of urea reduced the cost of medium by 72.6%.\u0000\u0000Abstract\u0000Chlorella sp. is a potential microalgae species to be produced commercially for feed, growth accelerator, and immuno-modulator in fish and shrimp culture. This study aimed to evaluate the various nitrogen sources on the growth, biomass production, and biochemical composition of Chlorella sp. FNUB01. The nitrogen sources used in this study were urea (NH2)2CO, potassium nitrate (KNO3), and ammonium nitrate (NH4NO3). Sodium nitrate (NaNO3) was used as a control as it is a part of the commercial medium BG-11. Generally, the sources of nitrogen in the media affected the growth and chemical composition of Chlorella sp. FNUB01. This green microalga grew better in the urea-containing medium which accounted for 1.5 times the concentration of that cultured in BG-11 (40 x106 cells. mL-1). Meanwhile, this microalgae species experienced the lowest growth when cultured in NH4NO3-containing medium. The biomass productivity of Chlorella sp. FNUB01 cultured in urea (0.93 g.L-1) was comparable to those grown with NaNO3 as the N source. A similar pattern was recorded for protein, chlorophyll, and carotenoid content as these biochemical contents were affected by N availability in the medium. Urea was an alternative low-cost N source for the culture of Chlorella sp. FNUB01. Replacement of NaNO3 with urea could reduce the cost of the medium by 72.6%.","PeriodicalId":17760,"journal":{"name":"Jurnal Ilmiah Perikanan dan Kelautan","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47314828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
DNA Barcoding of Cardinalfish (Apogonidae) in Gilimanuk Bay, Bali, Indonesia 印度尼西亚巴厘岛Gilimanuk湾红雀鱼的DNA条形码研究
Q4 Agricultural and Biological Sciences Pub Date : 2023-05-31 DOI: 10.20473/jipk.vi.43336
I. Putra, G. Indrawan, E. Faiqoh
Highlight ResearchEight species of cardinalfish in Gilimanuk Bay were delineated using the barcoding method.This study provides the first nucleotide sequence for Ostorhinchus hartzfeldii in GenBank online database.The phylogenetic tree showed that each sample resided in a distinct cluster, indicating that the barcoding method efficiently differentiated at the species level.The mean genetic distance between genera within the family was 31.8-fold higher than the mean genetic distance within species.AbstractThe Apogonidae is estimated to consist of nearly 300 fish species, most of which inhabit coral reef areas. The lack of distinctive body markings and overlapping species distribution makes species assignment challenging. Therefore, this study aimed to delineate species and establish barcoding reference databases of Apogonidae in Gilimanuk Bay (Bali, Indonesia) using the Cytochrome Oxidase I (COI) gene of the mitochondrial DNA. A total of 22 fish tissue samples were extracted with 10% Chelex solution. BLAST analysis was performed and genetic differentiation between species was calculated. The phylogenetic tree was constructed using the Maximum Likelihood method and tree visualization was generated using iTOL V5. The morphology and genetic identification results based on the mitochondrial COI gene revealed eight species of seven genera, and one species was new to GenBank online database. This study was the first-ever addition of COI sequence for Ostorhinchus hartzfeldii into the GenBank database. The average K2P genetic distance within species and K2P distance between genera within the family were 0.60% and 19.10%, respectively. The mean genetic distance between genera within the family was 31.8-fold higher than the mean genetic distance within species. The phylogenetic tree showed that each sample resided in a distinct cluster, which indicates that DNA barcoding is a reliable and effective approach for species delimitation in Apogonidae fishes.
重点研究采用条形码法对吉利马努克湾的8种红雀鱼进行了区划。这项研究在GenBank在线数据库中提供了第一个关于哈氏Ostorhinchus的核苷酸序列。系统发育树显示,每个样本都位于一个不同的聚类中,这表明条形码方法在物种水平上有效地进行了分化。科内属间平均遗传距离是种内平均遗传距离的31.8倍。据估计,Apogonidae由近300种鱼类组成,其中大多数栖息在珊瑚礁区。由于缺乏独特的身体标记和重叠的物种分布,物种分配具有挑战性。因此,本研究旨在利用线粒体DNA中的细胞色素氧化酶I(COI)基因来描述印度尼西亚巴厘岛吉里马努克湾Apogonidae的物种并建立条形码参考数据库。用10%的Chelex溶液提取了总共22个鱼类组织样品。进行BLAST分析并计算物种间的遗传分化。使用最大似然法构建系统发育树,并使用iTOL V5生成树可视化。基于线粒体COI基因的形态学和遗传鉴定结果揭示了7属8种,其中1种是GenBank在线数据库中的新物种。这项研究是首次将Ostorhinchus hartzfeldii的COI序列添加到GenBank数据库中。K2P在科内种间和属间的平均遗传距离分别为0.60%和19.10%。科内属间平均遗传距离是种内平均遗传距离的31.8倍。系统发育树表明,每个样本都位于一个不同的聚类中,这表明DNA条形码是一种可靠有效的Apogonidae鱼类物种划分方法。
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引用次数: 0
Comparison of Accounting Profit Between Offshore and Deep-sea Commercial Fishing Industry in Pahang State, East Coast of Peninsular Malaysia 马来西亚半岛东海岸彭亨州近海和深海商业渔业会计利润比较
Q4 Agricultural and Biological Sciences Pub Date : 2023-05-30 DOI: 10.20473/jipk.v15i2.42202
M. S. Sin, Lavaniya Gopabala Krishnan, S. Tai, A. Aziz, M. Kamaludin
Highlight ResearchEconomic performance of commercial fishing industry in Pahang State, East Coast of Peninsular Malaysia.Comparison of accounting profit between the two prominent fishing gear types: trawlers and purse seine in Pahang StateComparison of accounting profit between the different zones of fishing operations by the trawlers and purse seineResearch findings showed that trawlers give more profit than purse seine vessels in Pahang State and overcapacity and over exploitation is distinctly found in Pahang State commercial fishing industry.This study suggested that other analysis such as biological performance by this study area should be done in future coupled with the analysis of impact of management policies on the sustainability of the fishery.AbstractMarine capture fisheries in Malaysia plays prominent role in economic growth and commercial fisheries makes great contribution to country’s economy. The commercial fishing industry in East Coast of Peninsular Malaysia are mainly operated by trawlers and purse seines which rises a major concern in managing and achieving the goal of sustainable fishery. This study aimed to account the differences of accounting profit level in commercial fishing industry in Pahang State, East Coast of Peninsular Malaysia. In this study, comparison of accounting profits using revenue and profit approach among trawl and purse seine fishery in two different zones: Zone C (offshore) and Zone C2 (deep sea) was carried out based on the intensive survey on fishing capacity and other economic factors. The survey was carried out with the commercial fishermen and total respondents of 156 fishers at landing port. The result showed that trawlers incurred low cost yet provided higher profit than doses by purse seine. Moreover, there are more trawlers vessels operating in the Pahang state fishing ground and the open access nature of the fishery like larger profit invites more effort to use in the fishery which is leading to the over exploitation of fishery resources and threatening the sustainable condition of the fishery. The findings of the research inform the possible impact of fishing profit to overcapacity in sustainable fishery management in Pahang State fishery.
重点研究彭亨州商业渔业的经济效益,马来西亚半岛东海岸。两种主要渔具类型的会计利润比较:彭亨州的拖网渔船和围网渔船不同捕鱼作业区的会计利润对比研究结果表明,彭亨州拖网渔船的利润高于围网渔船,产能过剩在彭亨州的商业捕鱼业中可以明显地发现这种开采。这项研究表明,该研究区域的生物性能等其他分析应在未来进行,同时分析管理政策对渔业可持续性的影响。摘要马来西亚的海洋捕捞业在经济增长中发挥着重要作用,商业渔业对该国经济做出了巨大贡献。马来西亚半岛东海岸的商业捕鱼业主要由拖网渔船和围网经营,这是管理和实现可持续渔业目标的主要问题。本研究旨在说明马来西亚半岛东海岸彭亨州商业捕鱼业会计利润水平的差异。本研究在对捕捞能力和其他经济因素进行深入调查的基础上,对C区(近海)和C2区(深海)两个不同区域的拖网和围网渔业采用收益法核算利润进行了比较。这项调查是对商业渔民和156名登陆港渔民进行的。结果表明,拖网渔船的成本较低,但利润高于围网。此外,彭亨州渔场有更多的拖网渔船在作业,渔业的开放性,如更大的利润,吸引了更多的努力用于渔业,这导致了渔业资源的过度开发,并威胁到渔业的可持续发展状况。研究结果表明,捕鱼利润可能对彭亨州渔业可持续渔业管理能力过剩产生影响。
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引用次数: 0
Phytochemicals and Antioxidant Activity of Microalgae Dunaliella salina and Botryococcus braunii 盐杜氏微藻和布劳氏芽孢球菌的植物化学成分及抗氧化活性
Q4 Agricultural and Biological Sciences Pub Date : 2023-05-30 DOI: 10.20473/jipk.v15i2.36740
A. Ridlo, D. Pringgenies, Ribka Anindita Br Perangin-angin, D. Ariyanto
Highlight ResearchThe methanolic extracts of  salinaand B. braunii contained alkaloids, steroids, triterpenoids, flavonoids, and saponins.Chlorophyll a, chlorophyll b, and carotenoids were most abundant in the ethyl acetate extracts of salinaand B. braunii.The maximum total phenolic content was observed in the n-hexane extract of  salina(61.28 mg GAE/g sample) and the ethyl acetate extract of B. braunii (46.94 mg GAE/g sample).The n-hexane extracts exhibited the highest antioxidant activity, whereas saponins were most abundant in the methanol extracts.AbstractMicroalgal species such as Dunaliella salina and Botryococcus braunii are reportedly rich in natural antioxidants and phytochemicals. This study aimed to determine the phytochemicals and the antioxidant activity of D. salina and B. braunii. Microalgal samples were obtained from the Brackish Water Cultivation Fisheries Center (BPBAP), Situbondo, East Java. The extracts were prepared using the multilevel maceration method. The antioxidant activity of the algal species was analyzed using 1,1-diphenyl-2-picrylhydraxyl (DPPH). Quantitative analysis revealed that D. salina and B. braunii contained antioxidants, indicated by the appearance of yellow spots on the purple background of the TLC plate. The n-hexane extract of D. salina exhibited the highest antioxidant activity with a half-maximal inhibitory concentration (IC50) of 443.28 ppm, 61.28 mg GAE/g sample of total phenolics, 0.106 mg/g of chlorophyll a, 0.165 mg/g of chlorophyll b, and 1,697 mol/g of carotenoids. In contrast, the ethyl acetate extract of B. braunii exhibited the highest antioxidant activity with an IC50 of 634.55 ppm, 46.94 mg GAE/g sample of total phenolics, 18.146 mg/g of chlorophyll a, 12.592 mg/g of chlorophyll b, and 4573 mol/g of carotenoids. The microalgal species used in this study exhibited extremely weak antioxidant activity.
重点研究盐藻和B的甲醇提取物。braunii含有生物碱、类固醇、三萜、黄酮和皂苷。叶绿素a、叶绿素b和类胡萝卜素在盐和b的乙酸乙酯提取物中含量最高。布拉尼。在盐藻的正己烷提取物(61.28mg GAE/g样品)和B的乙酸乙酯提取物中观察到最大的总酚含量。braunii(46.94 mg GAE/g样品)。正己烷提取物表现出最高的抗氧化活性,而皂苷在甲醇提取物中含量最高。据报道,微藻类如盐藻(Dunaliella salina)和布氏杆菌(Botryococcus braunii)富含天然抗氧化剂和植物化学物质。本研究旨在测定盐藻和B.salina的植物化学物质及其抗氧化活性。布拉尼。微藻样品取自东爪哇西图邦多Brackish水产养殖中心(BPBAP)。采用多级浸渍法制备提取物。利用1,1-二苯基-2-苦酰肼(DPPH)对藻类的抗氧化活性进行了分析。定量分析表明,盐藻和braunii含有抗氧化剂,TLC板的紫色背景上出现黄色斑点。盐藻正己烷提取物表现出最高的抗氧化活性,半数最大抑制浓度(IC50)为443.28ppm,总酚类物质为61.28mg GAE/g样品,叶绿素a为0.106mg/g,叶绿素b为0.165mg/g,类胡萝卜素为1697mol/g。相反,B.braunii的乙酸乙酯提取物表现出最高的抗氧化活性,IC50为634.55ppm,总酚类样品为46.94mg GAE/g,叶绿素a为18.146mg/g,叶绿素B为12.592mg/g,类胡萝卜素为4573mol/g。本研究中使用的微藻表现出极弱的抗氧化活性。
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引用次数: 0
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Jurnal Ilmiah Perikanan dan Kelautan
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