B. Basuki, A. Alimuddin, D. Soelistyowati, S. Nuryati
Highlight Research A molecular marker for shrimp selection in disease resistance. Single nucleotide polymorphisms (SNP) in the ALF gene strongly correlate with shrimp resistance to WSSV infection. Higher ALF gene expression in survivor shrimp. SNP as molecular marker inherited in the first generation (G1) shrimp. ARMS-PCR method successfully detect SNP in the shrimp ALF gene. Abstract The Pacific white shrimp (Litopenaeus vannamei) is Indonesia’s main export commodity, but its production is constrained by the white spot syndrome virus (WSSV). Selective breeding of disease-resistant broodstock based on single nucleotide polymorphism (SNP) in the anti-lipopolysaccharide factor (ALF) gene is an alternative strategy for solving the disease problem. This study aimed to detect the SNP g.455 A>G in the anti-lipopolysaccharide factor (ALF) shrimp gene, evaluate the correlation of SNP with WSSV-resistance trait, analyze the expression level of immunity genes and genotype frequencies of the WSSV-resistance population shrimp and analyze the SNP inheritance in the first generation of selected shrimp. A total of 120 individuals from 4 families were used to detect the SNP marker using tetra-primer amplification refractory mutation system-polymerase chain reaction (ARMS-PCR). The correlation of the SNP marker with survival rate (SR) was analyzed using a general linear model (GLM) between genotype frequencies and SR. Genotypic similarities between broodstock and pedigree were analyzed using Chi-square. SNP g.455 A>G was successfully detected using the ARMS-PCR method and had a strong correlation between the marker and SR (p-value of AA = 0.012; AG = 0.359, and GG = 0.001). The resistant population has significantly higher ALF and SOD gene expression levels and AA genotype frequency. The SNP marker was inherited, so the broodstock and pedigree have the same genotype frequencies according to chi-square analysis (χ2 = 0.46 and p-value = 0.497). These results suggested that the g.455 genotype AA could be selected to produce WSSV-resistant Pacific white shrimp.
{"title":"Identification of a Potential SNP Related to the Expression of Immune Genes and Its Possible Application to Selection of WSSV-Resistant Pacific White Shrimp (Litopenaeus vannamei)","authors":"B. Basuki, A. Alimuddin, D. Soelistyowati, S. Nuryati","doi":"10.20473/jipk.vi.39110","DOIUrl":"https://doi.org/10.20473/jipk.vi.39110","url":null,"abstract":"Highlight Research\u0000\u0000A molecular marker for shrimp selection in disease resistance.\u0000Single nucleotide polymorphisms (SNP) in the ALF gene strongly correlate with shrimp resistance to WSSV infection.\u0000Higher ALF gene expression in survivor shrimp.\u0000SNP as molecular marker inherited in the first generation (G1) shrimp.\u0000ARMS-PCR method successfully detect SNP in the shrimp ALF gene.\u0000\u0000Abstract\u0000The Pacific white shrimp (Litopenaeus vannamei) is Indonesia’s main export commodity, but its production is constrained by the white spot syndrome virus (WSSV). Selective breeding of disease-resistant broodstock based on single nucleotide polymorphism (SNP) in the anti-lipopolysaccharide factor (ALF) gene is an alternative strategy for solving the disease problem. This study aimed to detect the SNP g.455 A>G in the anti-lipopolysaccharide factor (ALF) shrimp gene, evaluate the correlation of SNP with WSSV-resistance trait, analyze the expression level of immunity genes and genotype frequencies of the WSSV-resistance population shrimp and analyze the SNP inheritance in the first generation of selected shrimp. A total of 120 individuals from 4 families were used to detect the SNP marker using tetra-primer amplification refractory mutation system-polymerase chain reaction (ARMS-PCR). The correlation of the SNP marker with survival rate (SR) was analyzed using a general linear model (GLM) between genotype frequencies and SR. Genotypic similarities between broodstock and pedigree were analyzed using Chi-square. SNP g.455 A>G was successfully detected using the ARMS-PCR method and had a strong correlation between the marker and SR (p-value of AA = 0.012; AG = 0.359, and GG = 0.001). The resistant population has significantly higher ALF and SOD gene expression levels and AA genotype frequency. The SNP marker was inherited, so the broodstock and pedigree have the same genotype frequencies according to chi-square analysis (χ2 = 0.46 and p-value = 0.497). These results suggested that the g.455 genotype AA could be selected to produce WSSV-resistant Pacific white shrimp.","PeriodicalId":17760,"journal":{"name":"Jurnal Ilmiah Perikanan dan Kelautan","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44430221","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ameer Farhan Mohd Arzaman, Hayatul Safrah Salleh, A. Embong, K. Kadhim, Norzamziah Afzainizam, Loi Hoang Huy Phuoc Pham, N. A. Abdul-Kadir, Nur Yuhanis Mohd Nasir
Highlight Research The COVID-19 pandemic has caused an increase in the price of marine resources and the income of coastal communities has been affected. The government and the authorities need to come out with several incentives to control this condition and the request for fish markets can be met customarily without rising prices and declining marine resources. Malaysian Fisheries Development Authority (LKIM) a body that has a direct link to the fishing industry needs to ensure that all fishermen's associations participate in the purchase and marketing of fishermen's catch. Adaptation of fish producers will pave the way for all coastal communities to develop more organized fishing systems and sufficient food sources Abstract COVID-19 has a significant impact on the fishery industry, economy, and food supply. The government of Malaysia imposes a Movement Control Order (MCO) to curb the spread of COVID-19 infection. Although the MCO implementation has benefits in terms of public health, it has affected the fishery industry and the livelihood of coastal communities in Malaysia. This study aimed to identify the impact of the COVID-19 pandemic on the fishery industry and their livelihoods during that period of calamity. Qualitative research and thematic analysis were employed to seek deeper information on the real situation that retards the fishing activities to the opt stage. COVID-19 has already revealed gaps in health care, the economy, and food systems. The findings obtained can provide recommendations to the government to provide initiatives to fishermen to help them maintain and improve their livelihood. However, this study has limitations as the number of respondents is relatively small due to the restrictions of movement allowed by the government. Future studies could be further enhanced in terms of the impact of COVID-19 after movement restrictions have been loosened and could use a quantitative approach to obtain significant data.
{"title":"Coronavirus (COVID-19) Impacts on Malaysian Fisheries Sectors: Lesson Learned from the Movement Control Order (MCO)","authors":"Ameer Farhan Mohd Arzaman, Hayatul Safrah Salleh, A. Embong, K. Kadhim, Norzamziah Afzainizam, Loi Hoang Huy Phuoc Pham, N. A. Abdul-Kadir, Nur Yuhanis Mohd Nasir","doi":"10.20473/jipk.vi.38774","DOIUrl":"https://doi.org/10.20473/jipk.vi.38774","url":null,"abstract":"Highlight Research\u0000\u0000The COVID-19 pandemic has caused an increase in the price of marine resources and the income of coastal communities has been affected.\u0000The government and the authorities need to come out with several incentives to control this condition and the request for fish markets can be met customarily without rising prices and declining marine resources.\u0000Malaysian Fisheries Development Authority (LKIM) a body that has a direct link to the fishing industry needs to ensure that all fishermen's associations participate in the purchase and marketing of fishermen's catch.\u0000Adaptation of fish producers will pave the way for all coastal communities to develop more organized fishing systems and sufficient food sources\u0000\u0000 \u0000Abstract\u0000COVID-19 has a significant impact on the fishery industry, economy, and food supply. The government of Malaysia imposes a Movement Control Order (MCO) to curb the spread of COVID-19 infection. Although the MCO implementation has benefits in terms of public health, it has affected the fishery industry and the livelihood of coastal communities in Malaysia. This study aimed to identify the impact of the COVID-19 pandemic on the fishery industry and their livelihoods during that period of calamity. Qualitative research and thematic analysis were employed to seek deeper information on the real situation that retards the fishing activities to the opt stage. COVID-19 has already revealed gaps in health care, the economy, and food systems. The findings obtained can provide recommendations to the government to provide initiatives to fishermen to help them maintain and improve their livelihood. However, this study has limitations as the number of respondents is relatively small due to the restrictions of movement allowed by the government. Future studies could be further enhanced in terms of the impact of COVID-19 after movement restrictions have been loosened and could use a quantitative approach to obtain significant data.","PeriodicalId":17760,"journal":{"name":"Jurnal Ilmiah Perikanan dan Kelautan","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48030911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Highlight Research Fusarium symbiont Agelas sponge has antibacterial activity against aureus and E. coli Multi Drug Resistant Fusarium can grow on modified media soursop juice (SJ) and ginger juice (GJ) and has different inhibitory abilities against bacteria test Fusarium bioactive compounds were analyzed and purified by HPLC Based on TLC visualization, one of the bioactive compounds contained in Fusarium is a steroid group Abstract Marine symbiont fungi, specifically, are likely to have antibacterial properties. The production of secondary metabolites in cultures is strongly influenced by nutrient availability. This study aimed to study the modified media for the cultivation of Fusarium sp. and its antibacterial bioactivity. Fusarium sp. was isolated from Agelas sp. sponge collected from Riung Sea, East South Nusa, Indonesia. In this study, the modified media was soursop juice (SJ), ginger juice (GJ), and sago (S). The fungal mycelium was cultured and scaled up for 7–14 days until the mycelium achieved maximum growth. A filtration funnel and HPLC were used to purify the bioactive compounds. The diffusion agar method was used to test antibacterial activity against the multi-drug resistant (MDR) Staphylococcus aureus and Escherichia coli. The results indicated that Fusarium sp. could grow on soursop juice and ginger juice but could not grow on sago media. The inhibition zone produced by the filtration fraction of Fusarium sp. from each media differed. The soursop juice media produced the largest inhibition zone against both S. aureus (11.56 mm ± 0.140) and E. coli (12.16 mm ± 0.094) at 100 μg/disc. The ginger juice (GJ) and soursop juice (SJ) media are promising as alternative culture media for Fusarium sp. The structure of the bioactive compound Fusarium sp. from GJ and MJ media culture can be further investigated using NMR.
{"title":"Modified Media for Fungi Symbiont Sponge Agelas sp. (Fusarium sp.) Cultivation against Multidrug-Resistant Bacteria","authors":"K. Wittriansyah, A. Trianto, O. Radjasa","doi":"10.20473/jipk.vi.38453","DOIUrl":"https://doi.org/10.20473/jipk.vi.38453","url":null,"abstract":"Highlight Research\u0000\u0000Fusarium symbiont Agelas sponge has antibacterial activity against aureus and E. coli Multi Drug Resistant\u0000Fusarium can grow on modified media soursop juice (SJ) and ginger juice (GJ) and has different inhibitory abilities against bacteria test\u0000Fusarium bioactive compounds were analyzed and purified by HPLC\u0000Based on TLC visualization, one of the bioactive compounds contained in Fusarium is a steroid group\u0000\u0000 \u0000Abstract\u0000Marine symbiont fungi, specifically, are likely to have antibacterial properties. The production of secondary metabolites in cultures is strongly influenced by nutrient availability. This study aimed to study the modified media for the cultivation of Fusarium sp. and its antibacterial bioactivity. Fusarium sp. was isolated from Agelas sp. sponge collected from Riung Sea, East South Nusa, Indonesia. In this study, the modified media was soursop juice (SJ), ginger juice (GJ), and sago (S). The fungal mycelium was cultured and scaled up for 7–14 days until the mycelium achieved maximum growth. A filtration funnel and HPLC were used to purify the bioactive compounds. The diffusion agar method was used to test antibacterial activity against the multi-drug resistant (MDR) Staphylococcus aureus and Escherichia coli. The results indicated that Fusarium sp. could grow on soursop juice and ginger juice but could not grow on sago media. The inhibition zone produced by the filtration fraction of Fusarium sp. from each media differed. The soursop juice media produced the largest inhibition zone against both S. aureus (11.56 mm ± 0.140) and E. coli (12.16 mm ± 0.094) at 100 μg/disc. The ginger juice (GJ) and soursop juice (SJ) media are promising as alternative culture media for Fusarium sp. The structure of the bioactive compound Fusarium sp. from GJ and MJ media culture can be further investigated using NMR.","PeriodicalId":17760,"journal":{"name":"Jurnal Ilmiah Perikanan dan Kelautan","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44143800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Highlight Research Paniculata leaf crude extract has antibacterial compounds Paniculata leaf crude extract can inhibit the growth of E. tarda bacteria Gouramy liver infected by tarda bacteria undergoes degeneration, congestion, and necrosis Treatment using paniculata leaf extract can improve Gouramy liver damage Abstract Edwardsiellosis caused by bacterium E. tarda is a serious challenge faced by Gourami farmers. This study aimed to determine the antibacterial activity of A. paniculata leaf crude extract on the growth of E. tarda bacteria and its effect on the histopathology of gourami liver which was carried out in two stages. In the first stage, the active compound was identified in the leaves of A. paniculata followed by the Minimum Inhibitory Concentration (MIC) test at five different doses and two controls. In-silico test was conducted to determine the antibacterial activity of crude extract of A. paniculata leaf. In the second stage, the Lethal Dosage (LD50) and Toxicity (LC50) tests were carried out. Histopathological test of gouramy liver was carried out by taking the liver of fish that had been exposed to E. tarda bacteria. The active compounds contained in the crude extract of A. paniculata leaf are flavonoids, alkaloids, tannins, triterpenoids, and saponins. The Lethal Dosage (LD50) was 106 CFU/ml, and a toxicity test showed a dose of 250 mg/L caused the most death compared to other treatments. Histopathological test of gouramy liver showed that crude extract of A. paniculata leaf could improve liver function optimally at a dose of 300 (mg/L). The results of this study indicate that crude extract of A. paniculata leaf can affect the histology profile of gouramy liver and can cause toxicity if used inappropriately.
{"title":"Effect of Andrographis paniculata Leaf Crude Extract Against Edwardsiella tarda Bacteria and Histopathological Profile of the Liver of Osphronemus gouramy Juvenil","authors":"Giri Pratama, A. Prajitno, T. D. Sulistiyati","doi":"10.20473/jipk.vi.38197","DOIUrl":"https://doi.org/10.20473/jipk.vi.38197","url":null,"abstract":"Highlight Research\u0000\u0000Paniculata leaf crude extract has antibacterial compounds\u0000Paniculata leaf crude extract can inhibit the growth of E. tarda bacteria\u0000Gouramy liver infected by tarda bacteria undergoes degeneration, congestion, and necrosis\u0000Treatment using paniculata leaf extract can improve Gouramy liver damage\u0000\u0000 \u0000Abstract\u0000Edwardsiellosis caused by bacterium E. tarda is a serious challenge faced by Gourami farmers. This study aimed to determine the antibacterial activity of A. paniculata leaf crude extract on the growth of E. tarda bacteria and its effect on the histopathology of gourami liver which was carried out in two stages. In the first stage, the active compound was identified in the leaves of A. paniculata followed by the Minimum Inhibitory Concentration (MIC) test at five different doses and two controls. In-silico test was conducted to determine the antibacterial activity of crude extract of A. paniculata leaf. In the second stage, the Lethal Dosage (LD50) and Toxicity (LC50) tests were carried out. Histopathological test of gouramy liver was carried out by taking the liver of fish that had been exposed to E. tarda bacteria. The active compounds contained in the crude extract of A. paniculata leaf are flavonoids, alkaloids, tannins, triterpenoids, and saponins. The Lethal Dosage (LD50) was 106 CFU/ml, and a toxicity test showed a dose of 250 mg/L caused the most death compared to other treatments. Histopathological test of gouramy liver showed that crude extract of A. paniculata leaf could improve liver function optimally at a dose of 300 (mg/L). The results of this study indicate that crude extract of A. paniculata leaf can affect the histology profile of gouramy liver and can cause toxicity if used inappropriately.","PeriodicalId":17760,"journal":{"name":"Jurnal Ilmiah Perikanan dan Kelautan","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46811939","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
I. E. Susetya, M. Basyuni, Muhammad Rakezza Rifzy Rifzy, R. Amelia, Y. Bimantara, R. Leidonald, A. Fadhilah
Highlight Research We found 16 species of mollusks with a total of 15 species from the gastropod class and 1 species from the Bivalvia class bivalves in the silvofishery ponds, recording a total of 369 individuals. The density of mollusks in July in pond I was 3.6 ind/m2, while it was 10.66 ind/m2 in pond II. The mollusk diversity index in October after rehabilitation was included in the high diversity category because the value of H' was 1.6 - 3.0. Pond I has a diversity index value of 1.89 and pond II 1.82. The value of C-organic concentration in both ponds ranged from 3.00% to 5.72%, which is the optimum level for mollusk growth. Abstract Silvofishery is a system that combines mangrove and fishery to protect mangrove ecosystems and restore their functions, one of which is as a habitat for macrozoobenthos. Investigate the mollusks and mangrove density. Mollusk sampling was carried out on sub-plots measuring 1 x 1 m² in the mangrove vegetation transect, and mangrove density was measured by counting the number of trees directly on all of the mangrove species at two silvofishery ponds. A total of 16 species of mollusks were found at the observation site, consisting of gastropods and bivalves. The density of mollusks in July was 20 ind/m2 in pond I, while it recorded 9 ind/m2 in pond II. In October, after pond II rehabilitation, the density was inversely proportional to July, and the highest density was found in pond I at 10 ind/m2, while pond II measured 9 ind/m2. After rehabilitation, the index value H’ decreased in pond I and pond II from 2.82 and 2.98 to 2.52 and 2.68, respectively. The mangrove density was 887 ind/ha in pond I and 686 ind/ha in pond II. The substrate in both ponds was clay. Nitrate concentration values in the substrate in both ponds ranged from 0.27% to 0.29%, phosphate recorded a percentage of 0.07–0.09%, and C-organic ranged from 3.00% to 5.72%. The water's physicochemical parameters and the type and content of the substrate were still suitable for mollusk life.
{"title":"Mollusk Diversity in Percut Sei Tuan Silvofishery Ponds, North Sumatra, Indonesia","authors":"I. E. Susetya, M. Basyuni, Muhammad Rakezza Rifzy Rifzy, R. Amelia, Y. Bimantara, R. Leidonald, A. Fadhilah","doi":"10.20473/jipk.vi.37772","DOIUrl":"https://doi.org/10.20473/jipk.vi.37772","url":null,"abstract":"Highlight Research\u0000\u0000We found 16 species of mollusks with a total of 15 species from the gastropod class and 1 species from the Bivalvia class bivalves in the silvofishery ponds, recording a total of 369 individuals.\u0000The density of mollusks in July in pond I was 3.6 ind/m2, while it was 10.66 ind/m2 in pond II.\u0000The mollusk diversity index in October after rehabilitation was included in the high diversity category because the value of H' was 1.6 - 3.0. Pond I has a diversity index value of 1.89 and pond II 1.82.\u0000The value of C-organic concentration in both ponds ranged from 3.00% to 5.72%, which is the optimum level for mollusk growth.\u0000\u0000 \u0000Abstract\u0000Silvofishery is a system that combines mangrove and fishery to protect mangrove ecosystems and restore their functions, one of which is as a habitat for macrozoobenthos. Investigate the mollusks and mangrove density. Mollusk sampling was carried out on sub-plots measuring 1 x 1 m² in the mangrove vegetation transect, and mangrove density was measured by counting the number of trees directly on all of the mangrove species at two silvofishery ponds. A total of 16 species of mollusks were found at the observation site, consisting of gastropods and bivalves. The density of mollusks in July was 20 ind/m2 in pond I, while it recorded 9 ind/m2 in pond II. In October, after pond II rehabilitation, the density was inversely proportional to July, and the highest density was found in pond I at 10 ind/m2, while pond II measured 9 ind/m2. After rehabilitation, the index value H’ decreased in pond I and pond II from 2.82 and 2.98 to 2.52 and 2.68, respectively. The mangrove density was 887 ind/ha in pond I and 686 ind/ha in pond II. The substrate in both ponds was clay. Nitrate concentration values in the substrate in both ponds ranged from 0.27% to 0.29%, phosphate recorded a percentage of 0.07–0.09%, and C-organic ranged from 3.00% to 5.72%. The water's physicochemical parameters and the type and content of the substrate were still suitable for mollusk life.","PeriodicalId":17760,"journal":{"name":"Jurnal Ilmiah Perikanan dan Kelautan","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42718209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-08-30DOI: 10.20473/jipk.v14i2.33532
Clara Amelia Kusumawinahyu, Sharilla Aryananti Abidin, Patmawati, Dwi Yuli Pudjiastuti, Dwitha Nirmala, M. A. Alamsjah, L. Sulmartiwi, Raseetha Vani Siva Manikam
Highlight Research Experimental use of pre-treated pangasius skin as collagen raw material using different acetic acid immersion time Effects of immersion time on the yield of collagen from pangasius skin Effects of immersion time on the proximate content of collagen from pangasius skin Presenting SDS-Page profile of collagen from pangasius skin Abstract Pangasius sp. production in Indonesia has reached 384,310 tons in 2019. One of the main waste in pangasius fillet industry is the skin, which reached to 6% of body mass. Fish skins are alternative for making collagen because it has no restrictions for certain religions and ethnicities. Collagen is a protein biomaterial which acts as the main component of connective tissue. Extracting collagen using organic acids such as acetic acid is the most common extraction method. Herein, we report the effects of different immersion time of acetic acid to collagen properties from pangasius skin. In this study, pangasius skin was treated using 10% alcohol with the ratio 1:10 (w/v) for defatting and 0.1M NaOH with the ratio 1:10 (w/v) for eliminating non-collagenous protein. The immersion of 0.5M acetic acid was carried out on pangasius skin with the ratio of 1:20 (w/v), for 24, 48, and 72h at temperature less than 15oC. The sample was salted-out for 24 hours with NaCl until the concentration reached 2.5M, then the sample was freeze-dried at -40oC. This study investigated that longer immersion time affected the properties of pangasius skin collagen. Different immersion time significantly affected the yield of collagen from pangasius skin (p<0.05). Pangasius skin has a potential to be used as collagen raw material, and 72h of immersion time (P3) is suggested to produce pangasius skin collagen with the highest yield and protein content (6.15 % and 9.26 %). Production of collagen from the fish skin will contribute to increase pangasius waste valorization in pharmaceutical industry.
{"title":"Effects of Different Acetic Acid Immersion Time on the Properties of Collagen from Pangasius Skin","authors":"Clara Amelia Kusumawinahyu, Sharilla Aryananti Abidin, Patmawati, Dwi Yuli Pudjiastuti, Dwitha Nirmala, M. A. Alamsjah, L. Sulmartiwi, Raseetha Vani Siva Manikam","doi":"10.20473/jipk.v14i2.33532","DOIUrl":"https://doi.org/10.20473/jipk.v14i2.33532","url":null,"abstract":"Highlight Research\u0000\u0000Experimental use of pre-treated pangasius skin as collagen raw material using different acetic acid immersion time\u0000Effects of immersion time on the yield of collagen from pangasius skin\u0000Effects of immersion time on the proximate content of collagen from pangasius skin\u0000Presenting SDS-Page profile of collagen from pangasius skin\u0000\u0000 \u0000Abstract\u0000Pangasius sp. production in Indonesia has reached 384,310 tons in 2019. One of the main waste in pangasius fillet industry is the skin, which reached to 6% of body mass. Fish skins are alternative for making collagen because it has no restrictions for certain religions and ethnicities. Collagen is a protein biomaterial which acts as the main component of connective tissue. Extracting collagen using organic acids such as acetic acid is the most common extraction method. Herein, we report the effects of different immersion time of acetic acid to collagen properties from pangasius skin. In this study, pangasius skin was treated using 10% alcohol with the ratio 1:10 (w/v) for defatting and 0.1M NaOH with the ratio 1:10 (w/v) for eliminating non-collagenous protein. The immersion of 0.5M acetic acid was carried out on pangasius skin with the ratio of 1:20 (w/v), for 24, 48, and 72h at temperature less than 15oC. The sample was salted-out for 24 hours with NaCl until the concentration reached 2.5M, then the sample was freeze-dried at -40oC. This study investigated that longer immersion time affected the properties of pangasius skin collagen. Different immersion time significantly affected the yield of collagen from pangasius skin (p<0.05). Pangasius skin has a potential to be used as collagen raw material, and 72h of immersion time (P3) is suggested to produce pangasius skin collagen with the highest yield and protein content (6.15 % and 9.26 %). Production of collagen from the fish skin will contribute to increase pangasius waste valorization in pharmaceutical industry.","PeriodicalId":17760,"journal":{"name":"Jurnal Ilmiah Perikanan dan Kelautan","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42063840","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-08-30DOI: 10.20473/jipk.v14i2.34225
M. Murwantoko, Jamilah Hayati
Highlight Research The parasitic disease has been record in snakehead fish (Channa striata) from South Kalimantan The nematodes with needle shape on both tip end with approximately 1 mm length and moving inside of wall cysts were found in in abdomen cavity and flesh of fish The morphology of the nematode was observed by light- and scanning electron-microscope The analysis on 18S rRNA showed that this parasite is belonging to nematode Tanqua tiara Abstract Snakehead fish (Channa striata) is an important commodity in South Kalimantan Indonesia. The snakehead fish production was increased due to the capture and intensive culture. The disease is one of the obstacles for production that may happened in cultured- and wild-fishes. The aims of this study were to record and to identify parasite which infected on wild snakehead fish from Kandangan Lama, Panyipatan, Tanahlaut, South Kalimantan. The parasite identification was conducted based on the morphology and the molecular characters. The morphology was observed by light microscope and scanning electron microscope. The 18S rRNA of parasite was amplified using designed primers and followed by sequencing. Spherical cysts were found in abdomen cavity and flesh of snakehead fish. The cylindrical worm with needle shape on both tip end with approximately 1 mm length were moving inside of wall cysts. Alignment analysis of 18S rRNA showed the highest homology at 99.83% with Tanqua tiara. Phylogenic tree showed that this worm is located at distance clade with the nematodes that have been reported to infect snakehead fish. The morphology and molecular results verified that and first report the parasite found in snakehead fish in South Kalimantan was T. tiara species. This nematode parasite may be served as intermediate host.
{"title":"Record on Nematode Tanqua tiara Infection on Snakehead Fish Channa striata in South Kalimantan Indonesia","authors":"M. Murwantoko, Jamilah Hayati","doi":"10.20473/jipk.v14i2.34225","DOIUrl":"https://doi.org/10.20473/jipk.v14i2.34225","url":null,"abstract":"Highlight Research\u0000\u0000The parasitic disease has been record in snakehead fish (Channa striata) from South Kalimantan\u0000The nematodes with needle shape on both tip end with approximately 1 mm length and moving inside of wall cysts were found in in abdomen cavity and flesh of fish\u0000The morphology of the nematode was observed by light- and scanning electron-microscope\u0000The analysis on 18S rRNA showed that this parasite is belonging to nematode Tanqua tiara\u0000\u0000 \u0000Abstract\u0000Snakehead fish (Channa striata) is an important commodity in South Kalimantan Indonesia. The snakehead fish production was increased due to the capture and intensive culture. The disease is one of the obstacles for production that may happened in cultured- and wild-fishes. The aims of this study were to record and to identify parasite which infected on wild snakehead fish from Kandangan Lama, Panyipatan, Tanahlaut, South Kalimantan. The parasite identification was conducted based on the morphology and the molecular characters. The morphology was observed by light microscope and scanning electron microscope. The 18S rRNA of parasite was amplified using designed primers and followed by sequencing. Spherical cysts were found in abdomen cavity and flesh of snakehead fish. The cylindrical worm with needle shape on both tip end with approximately 1 mm length were moving inside of wall cysts. Alignment analysis of 18S rRNA showed the highest homology at 99.83% with Tanqua tiara. Phylogenic tree showed that this worm is located at distance clade with the nematodes that have been reported to infect snakehead fish. The morphology and molecular results verified that and first report the parasite found in snakehead fish in South Kalimantan was T. tiara species. This nematode parasite may be served as intermediate host. ","PeriodicalId":17760,"journal":{"name":"Jurnal Ilmiah Perikanan dan Kelautan","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48227688","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-08-30DOI: 10.20473/jipk.v14i2.34394
D. Ramadhani, A. Hendriana, D. Wahjuningrum, M. A. Mulya
Highlight Research Cinnamaldehyde for shrimp Dynamics of Vibrio and the correlation with shrimp health status Green and yellow Vibrio bacteria in water, intestine, and hepatopancreas of shrimp Cinnamaldehyde decrease the number of green Vibrio bacteria Abstract Disease can cause shrimp mortality and high economic losses caused by Vibriosis group. One alternative is using cinnamaldehyde which function as an anti-microbial and inhibit the production of toxins released by microorganisms. This study aimed to evaluate the administration of cinnamaldehyde on the dynamics of Vibrio bacteria and the health status of Pacific white shrimp fed with cinnamaldehyde-containing feed. This study used a completely randomized design (CRD) consisted of 4 treatments and 3 replications consisting of treatment K, A, B, and C which mean feeding without cinnamaldehyde, feeding containing cinnamaldehyde dose of 0.05%, 0.1%, and 0.2% respectively. Parameters included total bacteria (green and yellow Vibrio) in water, intestine, and hepatopancreas, as well as immune responses parameters, including total hemocyte count (THC), respiratory burst (RB), and phenol-oxidase (PO). All data were tabulated in Microsoft Excel 2016 and all statistical analysis was conducted in the SPSS v.22.0 software with one-way ANOVA followed by Duncan Multiple Range Test (DMRT (P=0.05). The results showed that the administration of cinnamaldehyde influences the dynamics of Vibrio and health status of shrimp. At the beginning and at the end of cinnamaldehyde administration, green and yellow Vibrio bacteria are mostly found in water, intestine, and hepatopancreas. The administration of cinnamaldehyde to Pacific white shrimp can reduce the number of green Vibrio bacteria both in the intestine and hepatopancreas as shown in treatments C and B. Cinnamaldehyde also affected the higher percentage of yellow Vibrio bacteria in treatments C and B compared to control (K). The highest immune responses of THC and RB were produced in treatments A and C compared to K. The decrease of green Vibrio bacteria and increased colonization of yellow Vibrio bacteria were correlated with immune responses. The best treatment in this study is treatment C.
{"title":"Vibrio Dynamics and Health Status of Pacific White Shrimp Fed with Cinnamaldehyde-Containing Feed","authors":"D. Ramadhani, A. Hendriana, D. Wahjuningrum, M. A. Mulya","doi":"10.20473/jipk.v14i2.34394","DOIUrl":"https://doi.org/10.20473/jipk.v14i2.34394","url":null,"abstract":"Highlight Research\u0000\u0000Cinnamaldehyde for shrimp\u0000Dynamics of Vibrio and the correlation with shrimp health status\u0000Green and yellow Vibrio bacteria in water, intestine, and hepatopancreas of shrimp\u0000Cinnamaldehyde decrease the number of green Vibrio bacteria\u0000\u0000 \u0000Abstract\u0000Disease can cause shrimp mortality and high economic losses caused by Vibriosis group. One alternative is using cinnamaldehyde which function as an anti-microbial and inhibit the production of toxins released by microorganisms. This study aimed to evaluate the administration of cinnamaldehyde on the dynamics of Vibrio bacteria and the health status of Pacific white shrimp fed with cinnamaldehyde-containing feed. This study used a completely randomized design (CRD) consisted of 4 treatments and 3 replications consisting of treatment K, A, B, and C which mean feeding without cinnamaldehyde, feeding containing cinnamaldehyde dose of 0.05%, 0.1%, and 0.2% respectively. Parameters included total bacteria (green and yellow Vibrio) in water, intestine, and hepatopancreas, as well as immune responses parameters, including total hemocyte count (THC), respiratory burst (RB), and phenol-oxidase (PO). All data were tabulated in Microsoft Excel 2016 and all statistical analysis was conducted in the SPSS v.22.0 software with one-way ANOVA followed by Duncan Multiple Range Test (DMRT (P=0.05). The results showed that the administration of cinnamaldehyde influences the dynamics of Vibrio and health status of shrimp. At the beginning and at the end of cinnamaldehyde administration, green and yellow Vibrio bacteria are mostly found in water, intestine, and hepatopancreas. The administration of cinnamaldehyde to Pacific white shrimp can reduce the number of green Vibrio bacteria both in the intestine and hepatopancreas as shown in treatments C and B. Cinnamaldehyde also affected the higher percentage of yellow Vibrio bacteria in treatments C and B compared to control (K). The highest immune responses of THC and RB were produced in treatments A and C compared to K. The decrease of green Vibrio bacteria and increased colonization of yellow Vibrio bacteria were correlated with immune responses. The best treatment in this study is treatment C.","PeriodicalId":17760,"journal":{"name":"Jurnal Ilmiah Perikanan dan Kelautan","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44406098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-08-30DOI: 10.20473/jipk.v14i2.32864
D. W. Kurniawidi, S. Alaa, Eva Nurhaliza, Desti Olga Safitri, S. Rahayu, Muhamad Ali, M. Amin
Highlight Research New method to synthesis nano chitosan from Vannamei shrimp with high Deacetylation Degree Nano chitosan from Vannamei shrimp can adsorb heavy metal Fe up to 81.35% Shrimp shells as an alternative adsorbents for Fe Abstract Shrimp cultivation produces shrimp wastes in several forms, including shells from the molting process. Shrimp shell waste can be used as a source of nano chitosan. Many researches have used nano chitosan for various applications, one of which is to adsorb heavy metal pollution. This present study aimed to extract chitosan from shrimp shells and investigate the ability of nano chitosan to adsorb Fe metal. The research began by isolating chitin and chitosan from shrimp shells through deproteinization, demineralization, and deacetylation. The obtained chitosan was afterward characterized using Fourier Transform Infrared (FTIR). Furthermore, the glassy ionic method was used to synthesize nano chitosan. Nano chitosan was characterized using a scanning electron microscope (SEM), and the Fe metal adsorption ability was measured using Atomic Absorption Spectroscopy (AAS). The FTIR results showed that the synthesized shrimp shells had successfully formed chitin compounds with the appearance of the C-O-C functional group, while the chitosan compounds with the appearance of O-H and N-H groups. The SEM characterization showed that nano chitosan with a size of 173.71 nm was successfully formed with identical morphology in the form of a flat (spherical), elongated, and irregular position. The nano chitosan was able to absorb Fe solution up to 81.35%. Therefore, nano chitosan from the shrimp shells is suitable as an adsorbent of heavy metal Fe.
{"title":"Synthesis and Characterization of Nano Chitosan from Vannamei Shrimp Shell (Litopenaeus vannamei)","authors":"D. W. Kurniawidi, S. Alaa, Eva Nurhaliza, Desti Olga Safitri, S. Rahayu, Muhamad Ali, M. Amin","doi":"10.20473/jipk.v14i2.32864","DOIUrl":"https://doi.org/10.20473/jipk.v14i2.32864","url":null,"abstract":"Highlight Research\u0000\u0000New method to synthesis nano chitosan from Vannamei shrimp with high Deacetylation Degree\u0000Nano chitosan from Vannamei shrimp can adsorb heavy metal Fe up to 81.35%\u0000Shrimp shells as an alternative adsorbents for Fe\u0000\u0000 \u0000Abstract\u0000Shrimp cultivation produces shrimp wastes in several forms, including shells from the molting process. Shrimp shell waste can be used as a source of nano chitosan. Many researches have used nano chitosan for various applications, one of which is to adsorb heavy metal pollution. This present study aimed to extract chitosan from shrimp shells and investigate the ability of nano chitosan to adsorb Fe metal. The research began by isolating chitin and chitosan from shrimp shells through deproteinization, demineralization, and deacetylation. The obtained chitosan was afterward characterized using Fourier Transform Infrared (FTIR). Furthermore, the glassy ionic method was used to synthesize nano chitosan. Nano chitosan was characterized using a scanning electron microscope (SEM), and the Fe metal adsorption ability was measured using Atomic Absorption Spectroscopy (AAS). The FTIR results showed that the synthesized shrimp shells had successfully formed chitin compounds with the appearance of the C-O-C functional group, while the chitosan compounds with the appearance of O-H and N-H groups. The SEM characterization showed that nano chitosan with a size of 173.71 nm was successfully formed with identical morphology in the form of a flat (spherical), elongated, and irregular position. The nano chitosan was able to absorb Fe solution up to 81.35%. Therefore, nano chitosan from the shrimp shells is suitable as an adsorbent of heavy metal Fe.","PeriodicalId":17760,"journal":{"name":"Jurnal Ilmiah Perikanan dan Kelautan","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49403917","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-08-30DOI: 10.20473/jipk.v14i2.33002
Naning Dwi Sulystyaningsih, N. Rahim, M. A. Liliyanti, E. Asrial, Rusmin Nuryadin, Nuri Muahiddah
Highlight Research Based on technological and economic dimensions, the sustainability of seaweed cultivation in Ekas Bay was less sustainable (index value lied between 26 and 50). The distance between seed clumps, drying places, seedling binding locations, and warehouses were the most sensitive attributes from technological dimension. Market status, market scale, and target market were the most sensitive attributes from economic dimension Abstract Indonesia is one of the major countries in the world that produces seaweed. West Nusa Tenggara has become one of the centers of seaweed producers in Indonesia because it has many bays that are used for seaweed cultivation activities. The problem of seaweed aquabusiness is the low productivity of the production unit managed by seaweed farmers. This study aimed to assess the effectiveness and production level of seaweed cultivation technology and the success of seaweed cultivation by seaweed farmers in Ekas Bay based on technological dimensions (17 attributes) and economics (14 attributes). The Rapsewaqua application is used to analyze the sustainability status of seaweed cultivation. Based on the results of the analysis, the index value was 50.08 for the technology dimension and 39.50 for the economic dimension. The result showed that the sustainability of seaweed in Ekas Bay was in the less sustainable category. Spacing between seed clumps, drying area, seedling binding location, and warehouse were the most sensitive attributes on the technological dimension. Market status, market scale, and target market were the most sensitive attributes in the economic dimension. These sensitive attributes need to be improved to support the sustainability of seaweed in Ekas Bay.
{"title":"Sustainability Evaluation of Seaweed Aquabusiness Management in Support of Sustainable Development Goal in Ekas Bay, East Lombok","authors":"Naning Dwi Sulystyaningsih, N. Rahim, M. A. Liliyanti, E. Asrial, Rusmin Nuryadin, Nuri Muahiddah","doi":"10.20473/jipk.v14i2.33002","DOIUrl":"https://doi.org/10.20473/jipk.v14i2.33002","url":null,"abstract":"Highlight Research\u0000\u0000Based on technological and economic dimensions, the sustainability of seaweed cultivation in Ekas Bay was less sustainable (index value lied between 26 and 50).\u0000The distance between seed clumps, drying places, seedling binding locations, and warehouses were the most sensitive attributes from technological dimension.\u0000Market status, market scale, and target market were the most sensitive attributes from economic dimension\u0000\u0000 \u0000Abstract\u0000Indonesia is one of the major countries in the world that produces seaweed. West Nusa Tenggara has become one of the centers of seaweed producers in Indonesia because it has many bays that are used for seaweed cultivation activities. The problem of seaweed aquabusiness is the low productivity of the production unit managed by seaweed farmers. This study aimed to assess the effectiveness and production level of seaweed cultivation technology and the success of seaweed cultivation by seaweed farmers in Ekas Bay based on technological dimensions (17 attributes) and economics (14 attributes). The Rapsewaqua application is used to analyze the sustainability status of seaweed cultivation. Based on the results of the analysis, the index value was 50.08 for the technology dimension and 39.50 for the economic dimension. The result showed that the sustainability of seaweed in Ekas Bay was in the less sustainable category. Spacing between seed clumps, drying area, seedling binding location, and warehouse were the most sensitive attributes on the technological dimension. Market status, market scale, and target market were the most sensitive attributes in the economic dimension. These sensitive attributes need to be improved to support the sustainability of seaweed in Ekas Bay.","PeriodicalId":17760,"journal":{"name":"Jurnal Ilmiah Perikanan dan Kelautan","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45946918","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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