Badrunanto Badrunanto, Shadila F Asoka, W. Wahyuni, Muhammad Farid, S. T. Wahyudi, Irmanida Batubara
Emprit and red ginger essential oils (EOs) are natural sources of antioxidants that have the potential to be used in cosmetics, one of which is as an anti-skin-aging. The aim of this study was to determine the component differences and anti-skin-aging potential of the two EOs. The components were determined by GC-MS, while discrimination was done by chemometric. The potential of the components as the anti-skin-aging were evaluated by molecular docking and molecular dynamics (MD) simulations. A total 66 components were identified in both EOs, where eucalyptol (17.92%) and camphene (15.12%) were the main component in emprit and red ginger, respectively. Chemometric analysis revealed two discriminant clusters highlighting their dissimilarity with germacrene D and α-zingiberene are the key markers for differentiation. The docking and MD simulations were demonstrated the four main components of emprit EO, namely α-curcumene, α-zingiberene, β-bisabolene and β-sesquiphellandrene, have the best docking scores and interact with the enzymes with a relatively stable interaction. AdmetSAR evaluation of the four components has shown good bioavailability and declared safe. This study succeeded in revealing two ginger EOs differences based on their components and demonstrated the emprit ginger EO was more promising as a natural anti-skin-aging agent for further research.
{"title":"Component Discrimination and Anti-skin-aging Potency of Emprit and Red Ginger Essential Oil: Chemometric, Molecular Docking and Molecular Dynamics Study","authors":"Badrunanto Badrunanto, Shadila F Asoka, W. Wahyuni, Muhammad Farid, S. T. Wahyudi, Irmanida Batubara","doi":"10.15408/jkv.v9i2.32765","DOIUrl":"https://doi.org/10.15408/jkv.v9i2.32765","url":null,"abstract":"Emprit and red ginger essential oils (EOs) are natural sources of antioxidants that have the potential to be used in cosmetics, one of which is as an anti-skin-aging. The aim of this study was to determine the component differences and anti-skin-aging potential of the two EOs. The components were determined by GC-MS, while discrimination was done by chemometric. The potential of the components as the anti-skin-aging were evaluated by molecular docking and molecular dynamics (MD) simulations. A total 66 components were identified in both EOs, where eucalyptol (17.92%) and camphene (15.12%) were the main component in emprit and red ginger, respectively. Chemometric analysis revealed two discriminant clusters highlighting their dissimilarity with germacrene D and α-zingiberene are the key markers for differentiation. The docking and MD simulations were demonstrated the four main components of emprit EO, namely α-curcumene, α-zingiberene, β-bisabolene and β-sesquiphellandrene, have the best docking scores and interact with the enzymes with a relatively stable interaction. AdmetSAR evaluation of the four components has shown good bioavailability and declared safe. This study succeeded in revealing two ginger EOs differences based on their components and demonstrated the emprit ginger EO was more promising as a natural anti-skin-aging agent for further research.","PeriodicalId":17786,"journal":{"name":"Jurnal Kimia Valensi","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139178011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
I. Ismiyarto, Qonita Mumtazati, Elmi Christi Julia Pandelaki, E. Fachriyah, N. Ngadiwiyana, P. R. Sarjono, N. Prasetya
Chitosan is a natural polymer that has antifungal activity. It is necessary to modify chitosan into its derivatives to increase its activity. One modification of chitosan that has the potential to be developed as an antifungal is carboxylated chitosan amide because this chitosan derivative contains a carboxylic group and is more hydrophilic. This research aims to synthesize chitosan amide carboxylate using several cyclic anhydride compounds and test its antifungal activity against Aspergillus flavus. The cyclic anhydrides used in this research are maleic anhydride and phthalic anhydride. In the initial stage of chitosan amide carboxylate synthesis, reaction optimization was carried out at varying temperatures of 25, 50, and 72oC for 7 hours. Compound characterization was carried out using FTIR and UV-Vis spectrophotometry. The disc diffusion method tested the chitosan amide carboxylate product for its antifungal activity against Aspergillus flavus. The optimal MCA (Maleoyl Chitosan Amide) product is (MCA_50), synthesized at a reaction temperature of 50oC. Under these optimal reaction conditions, PCA_50 (Pthaloyl Chitosan Amide) was successfully synthesized to produce a brownish-yellow solid with a yield of 46.1% (w/w) and a degree of substitution (DS) of 41.93%. The diameter of the inhibition zone against Aspergillus flavus for PCA_50 was 30 mm at the 12th hour of observation. The product (PCA_50) has better antifungal activity than chitosan and MCA_50.
{"title":"Synthesis of Carboxylated Chitosan Amide Using Some Cyclic Anhydride and Their Activities as Antifungal","authors":"I. Ismiyarto, Qonita Mumtazati, Elmi Christi Julia Pandelaki, E. Fachriyah, N. Ngadiwiyana, P. R. Sarjono, N. Prasetya","doi":"10.15408/jkv.v9i2.35244","DOIUrl":"https://doi.org/10.15408/jkv.v9i2.35244","url":null,"abstract":"Chitosan is a natural polymer that has antifungal activity. It is necessary to modify chitosan into its derivatives to increase its activity. One modification of chitosan that has the potential to be developed as an antifungal is carboxylated chitosan amide because this chitosan derivative contains a carboxylic group and is more hydrophilic. This research aims to synthesize chitosan amide carboxylate using several cyclic anhydride compounds and test its antifungal activity against Aspergillus flavus. The cyclic anhydrides used in this research are maleic anhydride and phthalic anhydride. In the initial stage of chitosan amide carboxylate synthesis, reaction optimization was carried out at varying temperatures of 25, 50, and 72oC for 7 hours. Compound characterization was carried out using FTIR and UV-Vis spectrophotometry. The disc diffusion method tested the chitosan amide carboxylate product for its antifungal activity against Aspergillus flavus. The optimal MCA (Maleoyl Chitosan Amide) product is (MCA_50), synthesized at a reaction temperature of 50oC. Under these optimal reaction conditions, PCA_50 (Pthaloyl Chitosan Amide) was successfully synthesized to produce a brownish-yellow solid with a yield of 46.1% (w/w) and a degree of substitution (DS) of 41.93%. The diameter of the inhibition zone against Aspergillus flavus for PCA_50 was 30 mm at the 12th hour of observation. The product (PCA_50) has better antifungal activity than chitosan and MCA_50.","PeriodicalId":17786,"journal":{"name":"Jurnal Kimia Valensi","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139178286","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Ansori, H. Kusuma, Fikaputri Rohmatul Maula, L. Qadariyah, M. Mahfud
Porang (Amorphophallus oncophyllus) is one of the local annual plants that contain high levels of oxalate. Oxalates are undesirable compounds in porang flour, and their levels must be removed or reduced to obtain high quality porang flour as a food ingredient. In this research, the extraction of oxalate acid was conducted using microwave-assisted solvent extraction and mechanical separation methods. This study aims to analysis the parameters that influence the extraction of oxalate acid using microwave-assisted solvent extraction such as the effect of microwave power, extraction time, feed-to-solvent ratio (F/S) and material size. The mechanical separation process will be studied the effect of material size for extraction of oxalate acid. The optimum yield obtained for oxalate acid extraction with microwave-assisted solvent extraction was microwave power of 600 W, extraction time of 30 min, feed-to-solvent ratio of 0.05 g/mL and porang size of 100 mesh obtained by oxalate acid yield of 24.78%. Porang size of 100 mesh was the optimum yield obtained for extraction of oxalate acid using mechanical separation method. The result of physical properties test using SEM-EDX and FTIR shows that extraction of oxalate acid from porang using microwave extraction method could produce oxalic acid which has good quality. In addition, extraction using microwave offers an environment-friendly extraction method, which can accelerate and increase the oxalate acid extraction yield.
{"title":"The Extraction of Oxalate Acid from Porang Flour (Amorphophallus oncophyllus) using Microwave-Assisted Solvent Extraction","authors":"A. Ansori, H. Kusuma, Fikaputri Rohmatul Maula, L. Qadariyah, M. Mahfud","doi":"10.15408/jkv.v9i2.34706","DOIUrl":"https://doi.org/10.15408/jkv.v9i2.34706","url":null,"abstract":"Porang (Amorphophallus oncophyllus) is one of the local annual plants that contain high levels of oxalate. Oxalates are undesirable compounds in porang flour, and their levels must be removed or reduced to obtain high quality porang flour as a food ingredient. In this research, the extraction of oxalate acid was conducted using microwave-assisted solvent extraction and mechanical separation methods. This study aims to analysis the parameters that influence the extraction of oxalate acid using microwave-assisted solvent extraction such as the effect of microwave power, extraction time, feed-to-solvent ratio (F/S) and material size. The mechanical separation process will be studied the effect of material size for extraction of oxalate acid. The optimum yield obtained for oxalate acid extraction with microwave-assisted solvent extraction was microwave power of 600 W, extraction time of 30 min, feed-to-solvent ratio of 0.05 g/mL and porang size of 100 mesh obtained by oxalate acid yield of 24.78%. Porang size of 100 mesh was the optimum yield obtained for extraction of oxalate acid using mechanical separation method. The result of physical properties test using SEM-EDX and FTIR shows that extraction of oxalate acid from porang using microwave extraction method could produce oxalic acid which has good quality. In addition, extraction using microwave offers an environment-friendly extraction method, which can accelerate and increase the oxalate acid extraction yield.","PeriodicalId":17786,"journal":{"name":"Jurnal Kimia Valensi","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139178135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Y. D. I. Siregar, Adi Riyadhi, Widya Anggun Damayanti, Rizkiansyah Rizkiansyah, Muhammad Bagas Murditya
Bio hydrocarbons are renewable fuels that can be produced through the catalytic deoxygenation of fatty acids, resulting in compositions like gasoline, kerosene, and diesel fractions derived from petroleum. The objective of this study is to generate gasoline, kerosene, and diesel from vegetable oils and animal fats using an MgO catalyst synthesized through the calcination of Mg-citrate. The characterization of the MgO catalyst, employing Fourier Transform Infrared (FTIR), X-Ray Diffraction (XRD) and Brunauer-Emmett-Teller (BET) surface adsorption method, revealed the presence of crystalline MgO and showed that mesoporous MgO with average pore size of 15.52 nm and exhibiting a surface area of 35.68 m2 g-1. The MgO catalyst was utilized in the deoxygenation reaction of palm oil, palm wax, and chicken fat, leading to the production of bio hydrocarbons with paraffin and olefin compositions like those found in gasoline, kerosene, and diesel derived from petroleum. Gas Chromatography-Mass Spectroscopy (GCMS) analysis of the liquid product demonstrated that the highest quantity of gasoline was derived from palm wax, followed by palm oil and chicken fat. Palm wax exhibits promising potential as a raw material for gasoline production through the deoxygenation reaction, specifically through decarboxylation and decarbonylation processes facilitated by the MgO catalyst.
{"title":"Production of Bio hydrocarbons from Vegetable Oils and Animal Fats Using Magnesium Oxide as Catalyst","authors":"Y. D. I. Siregar, Adi Riyadhi, Widya Anggun Damayanti, Rizkiansyah Rizkiansyah, Muhammad Bagas Murditya","doi":"10.15408/jkv.v9i2.30865","DOIUrl":"https://doi.org/10.15408/jkv.v9i2.30865","url":null,"abstract":"Bio hydrocarbons are renewable fuels that can be produced through the catalytic deoxygenation of fatty acids, resulting in compositions like gasoline, kerosene, and diesel fractions derived from petroleum. The objective of this study is to generate gasoline, kerosene, and diesel from vegetable oils and animal fats using an MgO catalyst synthesized through the calcination of Mg-citrate. The characterization of the MgO catalyst, employing Fourier Transform Infrared (FTIR), X-Ray Diffraction (XRD) and Brunauer-Emmett-Teller (BET) surface adsorption method, revealed the presence of crystalline MgO and showed that mesoporous MgO with average pore size of 15.52 nm and exhibiting a surface area of 35.68 m2 g-1. The MgO catalyst was utilized in the deoxygenation reaction of palm oil, palm wax, and chicken fat, leading to the production of bio hydrocarbons with paraffin and olefin compositions like those found in gasoline, kerosene, and diesel derived from petroleum. Gas Chromatography-Mass Spectroscopy (GCMS) analysis of the liquid product demonstrated that the highest quantity of gasoline was derived from palm wax, followed by palm oil and chicken fat. Palm wax exhibits promising potential as a raw material for gasoline production through the deoxygenation reaction, specifically through decarboxylation and decarbonylation processes facilitated by the MgO catalyst.","PeriodicalId":17786,"journal":{"name":"Jurnal Kimia Valensi","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139177623","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Septian, G. A. Wardani, R. Mardianingrum, R. Ruswanto
Cancer is defined as a disease caused by progressive and abnormal cell proliferation in the body. This condition is caused by deoxyribonucleic acid (DNA) changes, which causes cells to lose their normal function. The aim of this study is to find that flavonoid compounds have a more stable interaction than tamoxifen as anti-cancer candidates. Research has been conducted in silico with molecular docking (AutodockTools-1.5.7) and molecular dynamics of 200 flavonoid compounds. Furthermore, pharmacokinetic parameters, toxicity, and the application of the Lipinski Rule of Five were investigated. Based on molecular docking results, the compounds eriocotrin, glabrol, kaempferitrin, linarin, and narirutin have more stable interactions with lower binding energy (∆G) than tamoxifen. From the results of molecular docking, molecular dynamics, and pharmacokinetic studies, it is predicted that the kaempferitrin compound can be used as an anti-cancer candidate and does not cause toxicity through further research.
{"title":"The Virtual Screening of Flavonoid Derivatives on Progesterone, Estrogen, and HER-2 Receptor for Breast Cancer Treatment Candidate","authors":"A. Septian, G. A. Wardani, R. Mardianingrum, R. Ruswanto","doi":"10.15408/jkv.v9i1.31482","DOIUrl":"https://doi.org/10.15408/jkv.v9i1.31482","url":null,"abstract":"Cancer is defined as a disease caused by progressive and abnormal cell proliferation in the body. This condition is caused by deoxyribonucleic acid (DNA) changes, which causes cells to lose their normal function. The aim of this study is to find that flavonoid compounds have a more stable interaction than tamoxifen as anti-cancer candidates. Research has been conducted in silico with molecular docking (AutodockTools-1.5.7) and molecular dynamics of 200 flavonoid compounds. Furthermore, pharmacokinetic parameters, toxicity, and the application of the Lipinski Rule of Five were investigated. Based on molecular docking results, the compounds eriocotrin, glabrol, kaempferitrin, linarin, and narirutin have more stable interactions with lower binding energy (∆G) than tamoxifen. From the results of molecular docking, molecular dynamics, and pharmacokinetic studies, it is predicted that the kaempferitrin compound can be used as an anti-cancer candidate and does not cause toxicity through further research.","PeriodicalId":17786,"journal":{"name":"Jurnal Kimia Valensi","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46294450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Y. Susanti, Fariati Fariati, Effendy Effendy, Yulyani Nur Azizah
Cadmium (II) halides with N,N'-diethylthiourea (detu) ligands at a stoichiometry of 1: 2 tend to form molecular complexes [Cd(detu)2X2] with a distorted tetrahedral geometry at the central atom. Generally, these complex compounds are prepared by the conventional method of reflux for 4 hour. The use of ultrasonic waves for complex synthesis can be an alternative to make the reaction time more efficient and environmentally friendly. The aim of this study was to synthesize and characterize complex compounds from CdBr2 and detu ligands using the ultrasonication method that have not previously reported. The synthesis of complex compounds was carried out by reacting CdBr2 and detu (1:2) in methanol solvent. In the synthesized compounds, a melting point test, electrical conductivity test, Fourier Transform Infrared (FTIR), Scanning Electron Microscope-Energy Dispersive Xray (SEM-EDX), qualitative test of bromide ion and calculation of free energy using Spartan'14 software were carried out for the complex structure prediction. The complex compound resulted has colorless needle crystals with a melting point of 93-94°C. The results of the EDX analysis provide the empirical formula C10H24CdBr2N4S2. The electrical conductivity test data and the bromide ion qualitative test proved that the synthesized complex compound was a molecular complex compound with the molecular formula [Cd(detu)2Br2]. The complex compound has two possible structures, namely a distorted tetrahedral and a square planar. Free energy calculations showed that complex compounds with a distorted tetrahedral structure and a square planar have free energies of -527.5574 kJ/mol and -408.7424 kJ/mol, respectively.
{"title":"Structure Prediction and Ultrasonic Assisted Synthesis of Coordination Compound Cadmium (II) Bromide with N,N’-diethylthiourea Ligand","authors":"Y. Susanti, Fariati Fariati, Effendy Effendy, Yulyani Nur Azizah","doi":"10.15408/jkv.v9i1.30868","DOIUrl":"https://doi.org/10.15408/jkv.v9i1.30868","url":null,"abstract":"Cadmium (II) halides with N,N'-diethylthiourea (detu) ligands at a stoichiometry of 1: 2 tend to form molecular complexes [Cd(detu)2X2] with a distorted tetrahedral geometry at the central atom. Generally, these complex compounds are prepared by the conventional method of reflux for 4 hour. The use of ultrasonic waves for complex synthesis can be an alternative to make the reaction time more efficient and environmentally friendly. The aim of this study was to synthesize and characterize complex compounds from CdBr2 and detu ligands using the ultrasonication method that have not previously reported. The synthesis of complex compounds was carried out by reacting CdBr2 and detu (1:2) in methanol solvent. In the synthesized compounds, a melting point test, electrical conductivity test, Fourier Transform Infrared (FTIR), Scanning Electron Microscope-Energy Dispersive Xray (SEM-EDX), qualitative test of bromide ion and calculation of free energy using Spartan'14 software were carried out for the complex structure prediction. The complex compound resulted has colorless needle crystals with a melting point of 93-94°C. The results of the EDX analysis provide the empirical formula C10H24CdBr2N4S2. The electrical conductivity test data and the bromide ion qualitative test proved that the synthesized complex compound was a molecular complex compound with the molecular formula [Cd(detu)2Br2]. The complex compound has two possible structures, namely a distorted tetrahedral and a square planar. Free energy calculations showed that complex compounds with a distorted tetrahedral structure and a square planar have free energies of -527.5574 kJ/mol and -408.7424 kJ/mol, respectively.","PeriodicalId":17786,"journal":{"name":"Jurnal Kimia Valensi","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45194136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ahmad Zakaria, Aynuddin Aynuddin, Wittri Djasmasari
Various industrial activities produce heavy metals as by-products or wastes. Lead is a metal trace element from sewage disposal, vehicle fumes, and atmospheric emissions from industrial activities. Mango seeds are composed of cellulose, lignin, and hemicellulose, which have many potential binding groups. Mango seeds, which are often considered waste, can be utilized as a low-cost biosorbent due to their various functional groups, such as hydroxyl, carboxyl, carbonyl, alcohol, amide, and aromatic groups. Mango seed are organic waste with potential as low-cost biosorbent for heavy metals removal. The purposes of the study are: (1) to explore the optimum condition of biosorbent in absorbing heavy metal species (Pb (II)), and (2) to analyze the adsorption model of synthetic waste containing Pb(II). Mango seeds have a considerable potential to be used as a biosorbent to absorb heavy metal ions. The optimum conditions for Pb2+ ion adsorption is at pH 6 with contact time 70 minutes, and concentration of biosorbent 2.0 g/L. Adsorbate adsorption follows the Freundlich model, and adsorption occurs only in a few surface layers. The kinetic parameters of adsorbent satisfied pseudo-second-order reaction. The optimum adsorption capacity (qm) of mango seed biosorbent in absorbing Pb ions is 43.86 mg/g.
{"title":"Exploring The Potential of Mango Seed as A Bioadsorbent for Pb(II) Removal in Aqueous Solution","authors":"Ahmad Zakaria, Aynuddin Aynuddin, Wittri Djasmasari","doi":"10.15408/jkv.v9i1.31733","DOIUrl":"https://doi.org/10.15408/jkv.v9i1.31733","url":null,"abstract":"Various industrial activities produce heavy metals as by-products or wastes. Lead is a metal trace element from sewage disposal, vehicle fumes, and atmospheric emissions from industrial activities. Mango seeds are composed of cellulose, lignin, and hemicellulose, which have many potential binding groups. Mango seeds, which are often considered waste, can be utilized as a low-cost biosorbent due to their various functional groups, such as hydroxyl, carboxyl, carbonyl, alcohol, amide, and aromatic groups. Mango seed are organic waste with potential as low-cost biosorbent for heavy metals removal. The purposes of the study are: (1) to explore the optimum condition of biosorbent in absorbing heavy metal species (Pb (II)), and (2) to analyze the adsorption model of synthetic waste containing Pb(II). Mango seeds have a considerable potential to be used as a biosorbent to absorb heavy metal ions. The optimum conditions for Pb2+ ion adsorption is at pH 6 with contact time 70 minutes, and concentration of biosorbent 2.0 g/L. Adsorbate adsorption follows the Freundlich model, and adsorption occurs only in a few surface layers. The kinetic parameters of adsorbent satisfied pseudo-second-order reaction. The optimum adsorption capacity (qm) of mango seed biosorbent in absorbing Pb ions is 43.86 mg/g.","PeriodicalId":17786,"journal":{"name":"Jurnal Kimia Valensi","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45480049","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Waste is material that is disposed of from sources of human and natural activities that do not yet have economic value. Physically, vegetable waste is perishable due to its high-water content, especially Chinese Cabbage waste. The purpose of this study was the isolation and identification of lactic acid bacteria from Chinese Cabbage waste. Isolation was carried out to obtain isolates of lactic acid bacteria from Chinese Cabbage waste. Then it was amplified using a Polymerase Chain Reaction (PCR) instrument. The results of chromosomal DNA amplification of bacterial isolates from mustard 1, mustard 2 and mustard 3 waste showed DNA bands with a size of ± 1200 bp, so it can be concluded that the process of amplification of the 16s rRNA gene fragment in mustard 1, mustard 2 and mustard 3 isolates was successful. The results of the 16s rRNA base sequence analysis showed that the mustard 1 isolate had a similarity index of 84.65% with Bacillus sp, the gene for the mustard 2 isolate had a similarity index of 84.09% with the Uncultured bacterium clone, and the mustard 3 isolate gene had a similarity index of 85.42% with Environmental 16s rDNA sequence.
{"title":"Isolation and Identification of Lactic Acid Bacteria form Chinese Cabbage Waste by 16s rRNA Amplification using The Polymerase Chain Reaction (PCR) Method","authors":"A. Junitasari, T. D. Rosahdi, Yuni Siti Lestari","doi":"10.15408/jkv.v9i1.29466","DOIUrl":"https://doi.org/10.15408/jkv.v9i1.29466","url":null,"abstract":"Waste is material that is disposed of from sources of human and natural activities that do not yet have economic value. Physically, vegetable waste is perishable due to its high-water content, especially Chinese Cabbage waste. The purpose of this study was the isolation and identification of lactic acid bacteria from Chinese Cabbage waste. Isolation was carried out to obtain isolates of lactic acid bacteria from Chinese Cabbage waste. Then it was amplified using a Polymerase Chain Reaction (PCR) instrument. The results of chromosomal DNA amplification of bacterial isolates from mustard 1, mustard 2 and mustard 3 waste showed DNA bands with a size of ± 1200 bp, so it can be concluded that the process of amplification of the 16s rRNA gene fragment in mustard 1, mustard 2 and mustard 3 isolates was successful. The results of the 16s rRNA base sequence analysis showed that the mustard 1 isolate had a similarity index of 84.65% with Bacillus sp, the gene for the mustard 2 isolate had a similarity index of 84.09% with the Uncultured bacterium clone, and the mustard 3 isolate gene had a similarity index of 85.42% with Environmental 16s rDNA sequence.","PeriodicalId":17786,"journal":{"name":"Jurnal Kimia Valensi","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48257898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Bella Sukma Mahadika, A. Saefumillah, Saepurahman Saepurahman, K. A. Kurnia, D. V. Wellia
Dyes have a complex molecular structure that is difficult to degrade and can persist for a long time in water bodies. This study examines more thoroughly the effectiveness of photodegradation-adsorption of dyes, kinetic aspects, isotherms, thermodynamics, identification of the structure of photodegradation products, and changes in environmental quality parameter values whose results are quite satisfactory. The combination of photodegradation-adsorption of dyes by TiO2-chitosan modified glass beads using a flow system has never been accomplished before. Flowing of the dye resulted in a dye degradation efficiency of 99%. The FESEM-EDX analysis shows that TiO2-chitosan has been coated onto the surface of the glass beads. R2 of kinetics MB, MO, RB are 0.99234, 0.9889, 0.9971, which were pseudo-first order. Qm, KL, R2 are 6.4382, 0.70533, 0.99923; 16.7364, 1.42059, 0.98816; 6.0078, 1.06973, 0.99889, respectively. The ∆H, ∆S, ∆S of -9.388, -0.024, -1.979; -27.182, -0.078, -3.592; -4.819, -0.017, 0.464 indicate the exothermic and spontaneous reaction. COD, TOC, pH, turbidity values have reached the threshold required by the government. The degradation products formed during the photodegradation-adsorption process were identified using LC-MS that the structure of the dye has been degraded to become simpler so that it is safely disposed of into the environment.
{"title":"Characterization Study of Dyes Photodegradation-Adsorption Products by TiO2-Chitosan Immobilized on Glass Beads using Flow System","authors":"Bella Sukma Mahadika, A. Saefumillah, Saepurahman Saepurahman, K. A. Kurnia, D. V. Wellia","doi":"10.15408/jkv.v9i1.31855","DOIUrl":"https://doi.org/10.15408/jkv.v9i1.31855","url":null,"abstract":"Dyes have a complex molecular structure that is difficult to degrade and can persist for a long time in water bodies. This study examines more thoroughly the effectiveness of photodegradation-adsorption of dyes, kinetic aspects, isotherms, thermodynamics, identification of the structure of photodegradation products, and changes in environmental quality parameter values whose results are quite satisfactory. The combination of photodegradation-adsorption of dyes by TiO2-chitosan modified glass beads using a flow system has never been accomplished before. Flowing of the dye resulted in a dye degradation efficiency of 99%. The FESEM-EDX analysis shows that TiO2-chitosan has been coated onto the surface of the glass beads. R2 of kinetics MB, MO, RB are 0.99234, 0.9889, 0.9971, which were pseudo-first order. Qm, KL, R2 are 6.4382, 0.70533, 0.99923; 16.7364, 1.42059, 0.98816; 6.0078, 1.06973, 0.99889, respectively. The ∆H, ∆S, ∆S of -9.388, -0.024, -1.979; -27.182, -0.078, -3.592; -4.819, -0.017, 0.464 indicate the exothermic and spontaneous reaction. COD, TOC, pH, turbidity values have reached the threshold required by the government. The degradation products formed during the photodegradation-adsorption process were identified using LC-MS that the structure of the dye has been degraded to become simpler so that it is safely disposed of into the environment.","PeriodicalId":17786,"journal":{"name":"Jurnal Kimia Valensi","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43324690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
N. Nurdiani, D. Iswantini, N. Nurhidayat, W. Wahyuni, A. Kartono
Determination of alcohol content is very important in the food and beverage industry. Biosensor is an alternative to measuring ethanol content. Alcohol biosensors with a single microbe still have a narrow measurement area at ethanol concentrations, so a microbial consortium is needed to widen the range of measured ethanol concentrations. Therefore, it is necessary to screen the microbes from Bacillus sp and S. cerevisiae which have the potential to produce alcohol dehydrogenase (ADH) enzymes and optimize the consortium technique that can provide the best response to oxidation currents. In the yeast microbial screening of 14 S. cerevisiae isolates and 5 Bacillus sp isolates, it can be concluded that for the 14 yeast S. cerevisiae isolates that have the potential to produce ADH enzymes, there is one isolate with the SCRF code. For the 5 bacterial isolates of Bacillus sp that have the potential to produce ADH enzymes, all Bacillus isolates with the code Bacillus megatherium 29/9/14, Bacillus megatherium 23/6/22, Bacillus 6, Bacillus 53, and Bacillus 55. Based on the oxidation current data, Bacillus megatherium 23 /6/22 produces the highest current compared to other Bacillus isolates. The consortium technique that provides the highest current is the method of mixing 1:1 (µL) microbial suspension in an Eppendorf container. Optimization of the consortium's biofilms using the Response Surface Method was produced at 10 days of age, pH 7.5, and 75 µL of microbial suspension dripping volume.
{"title":"Screening and Optimization Consortium Technique of the Bacillus megatherium and Saccharomyces cerevisiae Microbial Consortium for Ethanol Detection","authors":"N. Nurdiani, D. Iswantini, N. Nurhidayat, W. Wahyuni, A. Kartono","doi":"10.15408/jkv.v9i1.31506","DOIUrl":"https://doi.org/10.15408/jkv.v9i1.31506","url":null,"abstract":"Determination of alcohol content is very important in the food and beverage industry. Biosensor is an alternative to measuring ethanol content. Alcohol biosensors with a single microbe still have a narrow measurement area at ethanol concentrations, so a microbial consortium is needed to widen the range of measured ethanol concentrations. Therefore, it is necessary to screen the microbes from Bacillus sp and S. cerevisiae which have the potential to produce alcohol dehydrogenase (ADH) enzymes and optimize the consortium technique that can provide the best response to oxidation currents. In the yeast microbial screening of 14 S. cerevisiae isolates and 5 Bacillus sp isolates, it can be concluded that for the 14 yeast S. cerevisiae isolates that have the potential to produce ADH enzymes, there is one isolate with the SCRF code. For the 5 bacterial isolates of Bacillus sp that have the potential to produce ADH enzymes, all Bacillus isolates with the code Bacillus megatherium 29/9/14, Bacillus megatherium 23/6/22, Bacillus 6, Bacillus 53, and Bacillus 55. Based on the oxidation current data, Bacillus megatherium 23 /6/22 produces the highest current compared to other Bacillus isolates. The consortium technique that provides the highest current is the method of mixing 1:1 (µL) microbial suspension in an Eppendorf container. Optimization of the consortium's biofilms using the Response Surface Method was produced at 10 days of age, pH 7.5, and 75 µL of microbial suspension dripping volume.","PeriodicalId":17786,"journal":{"name":"Jurnal Kimia Valensi","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45208319","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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