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Optimization of Pancreatic Lipase Inhibition in Fermented Beverages Brewed from Katuk Leaves Using RSM RSM法优化牛蒡叶发酵饮料中胰脂肪酶抑制作用
Pub Date : 2022-06-30 DOI: 10.6066/jtip.2022.33.1.28
Y. Maryati, A. Susilowati, Hani Mulyani, Aspiyanto, S. Budiari
The inhibition of pancreatic lipase and the associated reduction of lipid absorption has become the most appropriate approach for treating obesity. Meanwhile, katuk (Sauropus androgynus) leaves are rich in polyphenols that act as natural bioactive compounds and are also responsible for the potential effect on metabolic diseases, including inhibition of pancreatic lipase activity. Naturally occurring polyphenols can inhibit pancreatic lipase and consequently affect fat digestion as well as energy intake. Therefore, this study aimed to optimize the inhibitory activity of pancreatic lipase, which plays an essential role in lipid absorption. In fermented katuk (Sauropus androgynus) leaves were brewed following RSM using a Box Behnken design. Data analysis was used to optimize the formulation with a response surface model consisting of three factors namely sucrose concentration ranging from 10-20% b/v, culture 10-20 b/v, as well as fermentation time of 1-5 days. The parameters tested were the percentage inhibition of pancreatic lipase, antioxidant activity, polyphenol, and total acid. Based on the RSM optimization results obtained from the three influencing factors, the optimum conditions were established namely 10.43% w/v sucrose, 10% v/v kombucha culture, and fermentation for 5 days. These conditions yielded the most optimal responses, with the percentage inhibition of pancreatic lipase, antioxidant activity, polyphenol, and total acid being 86.13%, 96.27%, 0.97 mg GAE/mL, and 1.11%, respectively. In general, the results demons-trated that the RSM method of the Box Behnken design and the parameter prediction values obtained using the model equation are in good agreement with the experimental values with at least R2 ≥ 0.8.
抑制胰脂肪酶和相关的脂质吸收减少已成为治疗肥胖最合适的方法。同时,katuk (Sauropus androgynus)叶片富含多酚,多酚是一种天然的生物活性化合物,对代谢疾病也有潜在的影响,包括抑制胰腺脂肪酶活性。天然存在的多酚可以抑制胰腺脂肪酶,从而影响脂肪消化和能量摄入。因此,本研究旨在优化胰脂肪酶的抑制活性,胰脂肪酶在脂质吸收中起重要作用。在发酵katuk (Sauropus androgynus)中,使用Box Behnken设计按照RSM酿造叶子。通过数据分析,以蔗糖浓度10 ~ 20% b/v、培养10 ~ 20 b/v、发酵时间1 ~ 5 d为条件,建立响应面模型,对配方进行优化。测试了胰脂肪酶抑制率、抗氧化活性、多酚和总酸。根据三个影响因素的RSM优化结果,确定了最佳发酵条件:10.43% w/v蔗糖,10% v/v康普茶,发酵5 d。在此条件下,对胰脂肪酶、抗氧化活性、多酚和总酸的抑制率分别为86.13%、96.27%、0.97 mg GAE/mL和1.11%。结果表明,Box Behnken设计的RSM方法和模型方程得到的参数预测值与实验值吻合较好,R2≥0.8。
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引用次数: 1
Pengaruh Tepung Beras Pragelatinisasi terhadap Penyerapan Minyak dan Sensori Kue Cucur 磨砂对油的吸收和蛋糕的感官的影响
Pub Date : 2022-06-30 DOI: 10.6066/jtip.2022.33.1.1
Putri Novita Savitri, Elvira Syamsir, Slamet Budijanto
Cucur is a traditional food with oily appearance,which may affect the sensory acceptance. Thus, in this research we attempted to reduce its oil absorption by using pregelatinized rice flour as the ingredient for cucur dough. The type of rice and temperature of extrusion process affect the characteristics of pregelatinized rice flour and final products. Thus, the objective of this research was to determine the effect of using pregelatinized rice flour on the oil uptake and sensory of cucur. The type of rice used were IR64 and IR42, and the extrusion process temperatures were 130 and 150°C. The oil uptake by cucur made with pregelatinized rice flour was analyzed during deep frying and sensory analysis was done on the resulting cucur. The results showed that the type of rice, temperature of extrusion, and their interactions had a significant effect on the characteristics and sensory of cucur. A combination of IR42 and 150°C resulted in cucur with 35.97% moisture content, 19.32% fat content, 18.14% moisture loss, 18.14% oil uptake, while the overall sensory attribute was acceptable to the panelists. Use of pregelatinized rice flour resulted in decrease in the oil uptake and moisture loss. Cucur made with pregelatinized rice flour has desirable intensities of sensory attributes (appearance, flavor, texture, and mouthfeel), which result in the higher overall preferences score.
Cucur是一种油性的传统食品,可能会影响感官接受。因此,在本研究中,我们试图通过使用预糊化米粉作为葫芦面团的原料来减少其吸油量。大米的类型和挤压过程的温度影响预糊化米粉和最终产品的特性。因此,本研究的目的是确定使用预糊化米粉对cucur的吸油和感官的影响。使用的大米类型为IR64和IR42,挤压过程温度分别为130和150°C。在油炸过程中分析了预糊化米粉制成的cucur的吸油量,并对所得cucur进行了感官分析。结果表明,稻米类型、挤压温度及其相互作用对葫芦的特性和感官有显著影响。IR42和150°C的组合产生了水分含量为35.97%、脂肪含量为19.32%、水分损失为18.14%、油脂吸收为18.14%的cucur,而整体感官属性对小组成员来说是可接受的。预糊化米粉的使用降低了油脂的吸收和水分的损失。用预糊化米粉制成的Cucur具有理想的感官属性强度(外观、风味、质地和口感),这导致了更高的总体偏好得分。
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引用次数: 0
PREFERENSI KONSUMEN TERHADAP EMPAT VARIETAS UNGGUL MANGGA INDONESIA BERDASARKAN RESPON EMOSI 社区偏好消费与印尼长期向EMOSI报告
Pub Date : 2021-12-01 DOI: 10.6066/jtip.2021.32.2.116
R. Fitriyah, C. H. Wijaya, Budi Nurtama
Understanding the consumer preferences and needs for new products is really important to support the acceptance and marketing success of a product. This study associated the preferences with emotional responses to various ready-to-eat and fresh food products. The objective was to obtain the preference based on emotional responses from different superior mango cultivars named agri gardina 45 (AG), gadung 21 (GD), red garifta (GM) and orange garifta (GO). The preference profile was measured by the 9-point hedonic scale, while the emotional response by EsSense25 combined with rating questionnaire and 5-point scale. This study involved 108 panelists. The finding showed that consumers liked gadung and orange garifta, while slighlty liked agri gardina and red garifta. Additionally, the EsSense25’s response showed that 4 out of 25 emotional terms were significantly different among the cultivars, namely happy, joyful, satisfied, and disgusted. However, fifteen attributes among the 25 emotion terms strongly correlated with liking, with the strongest terms on joyful, happy, satisfied, interested, secure, and free. These six emotions were associated with garifta orange, one of the newly released cultivars. The results suggested that garifta orange is potentially well-accepted and commercialized widely.
了解消费者对新产品的偏好和需求对于支持产品的接受和营销成功非常重要。这项研究将偏好与对各种即食和新鲜食品的情绪反应联系起来。目的是从不同的优质芒果品种agri gardina 45(AG)、gadung 21(GD)、红色garifta(GM)和橙色garifta。偏好档案采用9分享乐量表测量,情绪反应采用EsSense25量表结合评分问卷和5分量表测量。这项研究涉及108名小组成员。调查结果显示,消费者喜欢gadung和橙色garifta,而轻视者则喜欢agri gardina和红色garifta。此外,EsSense25的反应显示,25个情绪术语中有4个在品种之间存在显著差异,即快乐、快乐、满足和厌恶。然而,在25个情感术语中,有15个属性与喜欢密切相关,其中快乐、快乐、满足、感兴趣、安全和自由的术语最强。这六种情绪与新发布的品种之一的garifta橙有关。研究结果表明,garifta橙具有被广泛接受和商业化的潜力。
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引用次数: 0
SIMPLEKS DAN MULTIPLEKS PRE-ENRICHMENT-PCR UNTUK DETEKSI Salmonella Enteritidis DAN Typhimurium PADA KARKAS AYAM
Pub Date : 2021-12-01 DOI: 10.6066/jtip.2021.32.2.148
Siti Nurjanah, W. Rahayu, R. Dewanti-Hariyadi, Ni Gusti Ayu Made Widyatari Asthiti, Rahadina Praba Melati
A PCR assay has been developed and applied to detect Salmonella contamination in chicken carcasses. However, a concentration fewer than 3 cells per gram lead to false-negative results due to difficulties in the DNA extraction. The objective of this study was to evaluate of the influence of pre-enrichment on the sensitivity of simplex and multiplex PCR methods the detection of for Salmonella spp., S. Enteritidis and S. Typhimurium in chicken carcasses. Artificial contamination was done using very low number of Salmonella Hadar, S. Enteritidis dan S. Typhimurium and pre-enrichment was carried out by 8 hours incubation in non-selective (BPW) medium. The results showed that simplex PCR could detect Salmonella spp., S. Enteritidis and S. Typhimurium at initial numbers of 2.3, 0.9 and 2.3 MPN/mL of cells in broth medium, respectively. A multiplex PCR could detect mixed culture of the three Salmonella serovars at an initial number of 0.73 MPN/mL of cells. When compared to non-enrichment treatment, simplex pre-enrichment-PCR gave an increase in the percentage of positive results in chicken carcasses (n= 12), from 75 to 100% for Salmonella spp., from 8 to 58% for S. Typhimurium, and from 58 to 75% for S. Enteritidis. Increasing in the positive percentage was also occurred at multiplex pre-enrichment-PCR, however the concentration of S. Enteritidis primer was not optimum for detection. Pre-enrichment step significantly increases the sensitivity of PCR-based assay for detection Salmonella.
已经开发了一种PCR检测方法,并将其应用于检测鸡胴体中的沙门氏菌污染。然而,由于DNA提取的困难,每克少于3个细胞的浓度导致假阴性结果。本研究的目的是评估预富集对单一和多重PCR方法检测鸡胴体中沙门氏菌、肠炎沙门氏菌和鼠伤寒沙门氏菌灵敏度的影响。人工污染是使用非常低数量的哈达尔沙门氏菌、肠炎沙门氏菌和鼠伤寒沙门氏菌进行的,并通过在非选择性(BPW)培养基中培养8小时进行预富集。结果表明,在肉汤培养基中,初始细胞数分别为2.3、0.9和2.3MPN/mL时,单纯形PCR可检测沙门氏菌、肠炎沙门氏菌和鼠伤寒沙门氏菌。多重PCR可以检测三种沙门氏菌血清型的混合培养,初始细胞数为0.73MPN/mL。与非富集处理相比,单纯预富集PCR使鸡胴体(n=12)的阳性结果百分比增加,沙门氏菌从75%增加到100%,鼠伤寒沙门氏菌由8%增加到58%,肠炎沙门氏菌则从58%增加到75%。多重预富集PCR的阳性率也有所增加,但肠炎沙门氏菌引物的浓度不是检测的最佳浓度。预富集步骤显著提高了基于PCR的检测沙门氏菌的灵敏度。
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引用次数: 0
PENGARUH KONSENTRASI MALTODEKSTRIN PADA ADONAN BEKU TINGGI PROTEIN TERHADAP KARAKTERISTIK ROTI maltodedestrin浓度对面包特性的高蛋白面团的影响
Pub Date : 2021-12-01 DOI: 10.6066/jtip.2021.32.2.126
D. Putri, Okta Pringga Pakpahan, Damat Damat, Ari Rizki Yuspita Ningrum, E. N. Santoso
The maltodextrin in frozen dough functions as the cryoprotectant to keep the structure of dough and yeast viability during freezing. This study investigated the optimum maltodextrin concentration in the high-protein frozen dough to obtain the best characteristics of sweet bread. The experimental design was a simple, completely randomized design (CRD) with one factor, i.e., maltodextrin concentrations of 0, 1, and 2% w/w. The experiment was done in triplicates with 0% maltodextrin as the control. During ten days of frozen storage, it was found that the addition of maltodextrin significantly affected the number of viable yeast after freezing. Furthermore, the addition of 2% maltodextrin produces the best bread characteristics. Furthermore, the addition of maltodextrin to the frozen dough also protects the water content during proofing which was shown by loaf volume 85.80% and water content of 15.30%. Furthermore, yeast viability and the gluten content in bread profoundly impacted the optimum elasticity crumb firmness value 2.00 N/mm².
冷冻面团中的麦芽糊精起到冷冻保护剂的作用,在冷冻过程中保持面团的结构和酵母的活力。研究了高蛋白冷冻面团中麦芽糊精的最佳浓度,以获得最佳的甜面包特性。实验设计是一个简单、完全随机的设计(CRD),有一个因素,即麦芽糊精浓度为0、1和2%w/w。实验以0%麦芽糊精为对照,分三次进行。在冷冻贮藏的10天中,发现添加麦芽糊精显著影响冷冻后活酵母的数量。此外,添加2%的麦芽糊精可产生最佳的面包特性。此外,在冷冻面团中添加麦芽糊精也可以保护发酵过程中的含水量,面包体积为85.80%,含水量为15.30%。此外,酵母活力和面包中的面筋含量深刻影响了最佳弹性面包屑硬度值2.00N/mm²。
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引用次数: 1
ISOLASI PROTEIN KACANG MERAH DAN KACANG HIJAU MENGGUNAKAN METODE ASAM BASA DIKOMBINASIKAN DENGAN PROSES ENZIMATIS 通过保存和保存保护进行蛋白质分离,使相关方法与ENZIMATIS过程相结合
Pub Date : 2021-12-01 DOI: 10.6066/jtip.2021.32.2.157
Slamet Hadi Kusumah, Robi Andoyo, Tita Rialita
Red beans (Phaseolus vulgaris L.) and greens beans (Phaseolus raditus L.) proteins contain high amount of essential amino acids lysine and leucine. The study aimed to determine the optimum conditions for protein isolation process from red beans and green beans flour to produce the highest protein content. Additionally, an enzymatic hydrolysis was aimed to produce isolates or protein concentrates of red beans and green beans with good digestibility. The research method used was the Response Surface Methodology (RSM) Box-Behnken Design with Design Expert 10. The variables used in this process were extraction temperature (30-50°C), extraction pH (8.50-9.50), and time extraction (30-60 minutes). The results showed that the optimum conditions for the extraction of red beans protein were extraction pH of 8.60, temperature of 30°C, and time of 30.1 minutes, with the resulting protein content of 86.88±1.38% with and a validity value of 0.91. Meanwhile, the optimum conditions for the green beans protein extraction process were extraction pH of 8.83, extraction temperature of 30°C, extraction time of 30 minutes which yielded protein content of 88.27±1.08% and a validity value of 0.97. Enzymatic hydrolysis using of 3% (w/w) bromelain enzyme on red bean and mung bean protein concentrate powder was able to increase protein digestibility by 15.61 and 14.51%, respectively.
红豆(Phaseolus vulgaris L.)和菜豆(Phaseuls raditus L.)蛋白质含有大量必需氨基酸赖氨酸和亮氨酸。本研究旨在确定从红豆和青豆粉中分离蛋白质的最佳条件,以产生最高的蛋白质含量。此外,酶水解旨在生产具有良好消化率的红豆和青豆的分离物或蛋白质浓缩物。使用的研究方法是响应面法(RSM)Box-Behnken设计与设计专家10。该过程中使用的变量是提取温度(30-50°C)、提取pH(8.50-9.50)和提取时间(30-60分钟)。结果表明,提取红豆蛋白的最佳条件是提取pH 8.60,温度30°C,时间30.1分钟,所得蛋白含量为86.88±1.38%,有效值为0.91。同时,青豆蛋白质提取工艺的最佳条件为提取pH 8.83,提取温度30°C,提取时间30分钟,蛋白质含量为88.27±1.08%,有效值为0.97。用3%(w/w)菠萝蛋白酶对红豆和绿豆浓缩蛋白粉进行酶解,蛋白质消化率分别提高15.61%和14.51%。
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引用次数: 0
AUTENTIKASI CEPAT MADU HUTAN KALIMATAN TIMUR DENGAN ATR-FTIR SPEKTROSKOPI KOMBINASI ANALISIS KEMOMETRIKA 分光镜组合分析凯氏计量学合格渔业的授权
Pub Date : 2021-12-01 DOI: 10.6066/jtip.2021.32.2.181
Y. Prayitno, Aswita Emmawati, Sulistyo Prabowo, K. Candra, Anton Rahmadi
Honey adulteration is mostly conducted by the addition of sucrose. In this study, the authentication of honey was conducted using ATR-FTIR and chemometrics. Pure honey samples (MA) were collected from nine regions in East Kalimantan. The ATR-FTIR spectra of these samples were then compared to sucrose-adulterated honey (MS), which were prepared in the sucrose concentration from 2.5 to 50% (v / v).The data analysis was performed using chemometrics techniques: 1) Principle Component Analysis (PCA) method, 2) classification with Discriminant Analysis (DA), and 3) regression with (PCR) and (PLS). As a result, PCA was able to visualize the differences between MS and MA. DA analysis was able to distinguish MS and MA at wave numbers from 1200 to 800 cm-1 with 92.5% performance index. Quantitative calibration models of the sucrose-adulterated honey could be obtained from PLS and PCR, while the best calibration model was obtained with the PLS method from the 2nd derivative spectra. In summary, sucrose-adulterated honey from East Kalimantan can be authenticated using ATR-FTIR method in combination with chemometric analysis.
蜂蜜掺假主要是通过添加蔗糖来进行的。在本研究中,使用ATR-FTIR和化学计量学对蜂蜜进行了鉴定。从东加里曼丹的九个地区采集了纯蜂蜜样品。然后将这些样品的ATR-FTIR光谱与在蔗糖浓度为2.5%至50%(v/v)下制备的掺蔗糖蜂蜜(MS)进行比较。数据分析使用化学计学技术进行:1)主成分分析(PCA)方法,2)判别分析(DA)分类,3)PCR和PLS回归。结果,PCA能够显示MS和MA之间的差异。DA分析能够在1200至800cm-1的波数下区分MS和MA,性能指数为92.5%。通过PLS和PCR可以得到掺蔗糖蜂蜜的定量校准模型,而通过二阶导数光谱的PLS方法可以得到最佳的校准模型。总之,东加里曼丹的蔗糖掺假蜂蜜可以使用ATR-FTIR方法结合化学计量分析进行鉴定。
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引用次数: 1
PHYSICAL CHARACTERISTICS, SENSORY EVALUATION, AND AMINO ACID CONTENT OF POWDERED OVER FERMENTED MLANDING TEMPEH 发酵粉的物理特性、感官评价及氨基酸含量
Pub Date : 2021-12-01 DOI: 10.6066/jtip.2021.32.2.190
A. Nursiwi, D. Ishartani, A. M. Sari, Nurani Istiqomah
Mlanding tempeh is a non-soybean tempeh which made from ripe lamtoro (Leucaena leucocephala) seed. In Wonogiri, an area in Indonesia over fermented mlanding tempeh (OFMT) often used as seasoning for traditional food because it produced special taste. OFMT is dried to extend the shelf life. The objectives of the research was to study the physical characteristics, sensory evaluation, and amino acid content of powdered over fermented mlanding tempeh (POFMT). The drying of the OFMT used a cabinet dryer with the drying temperature were 55, 60, and 65°C for 8 hours then ground into powder. The powder was analyzed for yield, water absorption capacity, solubility, bulk density, hedonic test, and amino acid content. The results showed that the different drying temperature had a significant effect on yield and water absorption capacity but had not significant effect on solubility and bulk density of the POFMT. The yied varies between 7.06-12.92% and the water absrption capacity varies between 0.36-0.55 g/g. While the solubility of the POFMT varies between 36.45-41.33% and the bulk density varies between 0.38-0.42 g/mL. The different drying temperature had not a significant effect on panelist’s preference. Amino acid content of POFMT varies between 0.99-5.43% (db), with the two highest content is glutamic acid and aspartic acid.
麦兰丁豆豉是由成熟的银合欢种子制成的非大豆豆豉。在印度尼西亚的沃诺吉里,过度发酵的豆沙(OFMT)经常被用作传统食物的调味品,因为它产生了特殊的味道。OFMT经过干燥以延长保质期。本研究的目的是研究粉末过发酵麦麸(POFMT)的物理特性、感官评价和氨基酸含量。OFMT的干燥使用机柜式干燥机,干燥温度分别为55、60和65°C,持续8小时,然后研磨成粉末。对粉末的产率、吸水能力、溶解度、堆积密度、特性测试和氨基酸含量进行了分析。结果表明,不同的干燥温度对POFMT的收率和吸水率有显著影响,但对其溶解度和堆积密度没有显著影响。yied在7.06-12.92%之间变化,吸水能力在0.36-0.55g/g之间变化。而POFMT的溶解度在36.45-41.33%之间变化,堆积密度在0.38-0.42g/mL之间变化。不同的干燥温度对小组成员的偏好没有显著影响。POFMT的氨基酸含量在0.99-5.43%(db)之间,其中谷氨酸和天冬氨酸含量最高。
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引用次数: 0
SENSORY PROFILE OF 3 IN 1 INSTANT COFFEE USING EMOTIONAL-SENSORY MAPPING, FLASH PROFILE, AND CATA (CHECK-ALL-THAT-APPLY) METHODS 使用情感-感官映射,闪光配置文件和数据(检查所有适用)方法的3合1速溶咖啡的感官配置文件
Pub Date : 2021-12-01 DOI: 10.6066/jtip.2021.32.2.169
D. Hunaefi, Wicensky Marusiva
Three in one instant coffee is popular among university students. A variety brands of such instant coffee are available on the market. However, the literature on the sensory aspects of the instant coffee is rather limited slim. The project aimed to test three-in-one instant coffee sachets for their sensory characteristics. Two methods, CATA (Attributes and Emotional Mapping) and Flash Profile (extension of Free Choice Profiling) were employed to generate the attribute profiles and emotional dashboard. Sixty IPB University students were selected as panelists. Sensory attributes terminologies and their relation to emotion were generated using Focus Group Discussion among instant coffee heavy drinker. Profile of coffee D showed a creamy texture, while sweetness and milky was dominant when it came to coffee B and coffee C. The creaminess in coffee G was strongly related to feelings of happiness in a consumer. In addition, the sweet and milky taste and flavor in coffee B was seen as a relaxing and luxurious feeling. A Preference Mapping was constructed, and it showed that sweetness, milky, and creaminess improved the liking of the student consumers that make coffee C the most preferred brand among the panelists. In short, the use of flash profiles and check-all-it-dilute/CATA (attributes and emotional) equipped with preference mapping can provide emotional attributes and dashboards that will be important to understand products more than just consumer liking.
三合一速溶咖啡在大学生中很受欢迎。市场上有各种品牌的这种速溶咖啡。然而,关于速溶咖啡感官方面的文献相当有限。该项目旨在测试三合一速溶咖啡小袋的感官特征。采用CATA(属性和情绪映射)和Flash Profile(自由选择评测的扩展)两种方法生成属性评测和情绪仪表板。60名IPB大学学生被选为小组成员。感官属性术语及其与情绪的关系是在大量饮用速溶咖啡的人群中通过焦点小组讨论产生的。咖啡D的质地呈奶油状,而咖啡B和咖啡C的甜味和乳白色占主导地位。咖啡G的奶油味与消费者的幸福感密切相关。此外,B咖啡中的甜味和乳白色味道被视为一种放松和奢华的感觉。构建了偏好映射,结果显示,甜味、乳白色和奶油味提高了学生消费者的喜好,使咖啡C成为小组成员中最喜欢的品牌。简言之,使用flash配置文件并检查其稀释程度/CATA(属性和情感),并配备偏好映射,可以提供情感属性和仪表盘,这对于理解产品而不仅仅是消费者的喜好非常重要。
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引用次数: 1
SANITASI ALERGEN PADA PROSES PRODUKSI BISKUIT DALAM UPAYA MENGURANGI RESIDU ALERGEN TELUR 饼干生产过程中的过敏原卫生,以减少鸡蛋的过敏原残留物
Pub Date : 2021-12-01 DOI: 10.6066/jtip.2021.32.2.136
Kartika Sari, Nurheni Sri Palupi, Puspo Edi Giriwono
Food allergy is a hypersensitive response to food, generally protein, triggered by body immune system. These responses can be mild, however in very rare cases may cause severe reactions and life threatening known as called anaphylaxis. Food allergy has been one of the main factors for product recall. It can happen due to many reasons, such as product package labeling fault, raw material labeling fault and cross contamination. Validation of the effectiveness of cleaning and sanitation process become a critical point to minimize cross contamination. This study aimed to determine the optimum chemical concentration and contact time to eliminate egg residue in biscuit production cleaning process, obtain valid and verified cleaning procedures, and produce cleaning procedures that can be implemented in a production line. The result showed that physical cleaning/pre-cleaning for 30 minute and using 1% (v/v) concentration of caustic based polybrite chemical cleaning for 3-minute contact time can effectively eliminate the egg residue on stainless steel and polyurethane surface equipment. Food contact surface material and product characteristics (wet dough, dry biscuit) affected the number of egg residue on the equipment. Packing area with food contact surface equipment made from stainless steel is not considered critical area for allergen sanitation. It is shown that prior to sanitation process, analysis of egg allergen residue has already given negative result. Validation process can prove the effectiveness of allergen sanitation process to prevent potential egg allergen cross contamination in biscuit production.
食物过敏是由身体免疫系统引发的对食物(通常是蛋白质)的过敏反应。这些反应可能是轻微的,但在极少数情况下可能会引起严重反应和危及生命的过敏反应。食物过敏一直是产品召回的主要因素之一。它的发生可能有多种原因,如产品包装标签错误、原材料标签错误和交叉污染。验证清洁和卫生过程的有效性成为最大限度减少交叉污染的关键。本研究旨在确定饼干生产清洁过程中消除鸡蛋残留物的最佳化学浓度和接触时间,获得有效且经过验证的清洁程序,并制定可在生产线上实施的清洁程序。结果表明,物理清洗/预清洗30分钟,使用1%(v/v)浓度的苛性碱基聚苯酯化学清洗3分钟接触时间,可以有效去除不锈钢和聚氨酯表面设备上的鸡蛋残留物。食物接触表面材料和产品特性(湿面团、干饼干)影响设备上鸡蛋残留物的数量。装有不锈钢食品接触表面设备的包装区域不被视为过敏原卫生的关键区域。结果表明,在卫生处理之前,对鸡蛋过敏原残留的分析已经给出了阴性结果。验证过程可以证明过敏原卫生过程的有效性,以防止饼干生产中潜在的鸡蛋过敏原交叉污染。
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引用次数: 1
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Jurnal Teknologi dan Industri Pangan
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