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Sensory Mapping of UHT Milk with Single-Origin Chocolate UHT牛奶与单一产地巧克力的感官图谱
Pub Date : 2023-06-27 DOI: 10.6066/jtip.2023.34.1.25
D. Hunaefi, Sures Setiadi Tarigan, Elisabeth Dwinawati, Z. Sholehuddin, F. M. Taqi, Yves Henri Marie Zeller
Like coffee, the popularity of speciality chocolate flavour or single-origin chocolate is growing in the Indonesian market. At the same time, sensory characteristic of the chocolate flavors is becoming more important, affecting both the taste and marketing of food product with chocolate flavor. As such, this study aims to describe UHT milk with single-origin chocolate through a complete sensory attribute mapping. A focus group discussion (FGD) resulted 15 texture attributes, 11 need-state attributes, 15 music attributes, 17 emotion attributes, 11 flavor attributes, and additional 70 color attributes. The complete sensory mapping test was performed using the CATA method based on attributes obtained from the FGD and analyzed using XLSTAT. The resulting product descriptions are as follows: cocoa Flores products were associated with soft and light textures, a need to focus and provide energy, pleasant classical and pop music, relaxed and warm emotions, flavors of ice cream, and light green, purple and brown colors. Cocoa Bali was linked to a smooth but rather hard texture, a need for rest and exercise, a cheerful musical style, happy emotions, a biscuit flavor, and a pink-yellow color. Cocoa Aceh was correlated with smooth and soft textures, state-of-the-art rest, energetic music, relaxed and energetic emotions, creamy flavor, and blue color. Meanwhile, cocoa Java was attributed with a smooth but rather hard texture, a state-of-the-art mood booster, cheerful music, relaxed emotions, ice cream flavor, and red-brown color. Thus, understanding specific sensory characteristics enables the producer to improve the product's development and marketing.
与咖啡一样,特色巧克力口味或单一产地巧克力在印尼市场越来越受欢迎。同时,巧克力口味的感官特征也越来越重要,影响着巧克力口味食品的口感和市场营销。因此,本研究旨在通过完整的感官属性映射来描述具有单一来源巧克力的UHT牛奶。焦点小组讨论(FGD)产生了15个纹理属性、11个需求状态属性、15个音乐属性、17个情感属性、11种风味属性和另外70个颜色属性。基于从FGD获得的属性,使用CATA方法进行完整的感官图谱测试,并使用XLSTAT进行分析。由此产生的产品描述如下:可可Flores产品质地柔软轻盈,需要专注并提供能量,令人愉快的古典音乐和流行音乐,轻松温暖的情绪,冰淇淋的味道,以及浅绿色、紫色和棕色。巴厘岛可可与光滑但相当坚硬的质地、休息和锻炼的需要、欢快的音乐风格、快乐的情绪、饼干味和粉黄色联系在一起。可可亚齐与光滑柔软的质地、最先进的休息、充满活力的音乐、放松而充满活力的情绪、奶油味和蓝色有关。与此同时,可可Java被认为具有光滑但相当坚硬的质地、最先进的情绪助推器、欢快的音乐、放松的情绪、冰淇淋的味道和红棕色。因此,了解特定的感官特征使生产商能够改进产品的开发和营销。
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引用次数: 0
Fingerprinting FTIR-ATR Fraksi Kopi Robusta dan Arabika serta Korelasinya terhadap Aktivitas Antioksidan Robusta和Arabika咖啡的微量成分以及它们与抗氧化剂活性的相互作用
Pub Date : 2023-06-21 DOI: 10.6066/jtip.2023.34.1.70
Masjuwina Simatupang, Dian Herawati, Nancy Dewi Yuliana
Coffee has a positive effect on health due to its high content of antioxidant compounds. The potential antioxidant activity of coffee is strongly influenced by its chemical compound profile. This study aimed to analyze the effect of different solvents on the chemical metabolites profile, antioxidant activity, and to determine the relevant chemical functional groups which positively contribute to the coffee’s antioxidant activity. In this study, methanolic extract of coffee samples from robusta and arabica varieties were fractionated by liquid-liquid fractionation method using four solvents with different polarities. ABTS (2,2'-azinobis (3-ethylbenzothiazoline-6-sulphonic acid) and cupric reducing antioxidant capacity (CUPRAC) assays were applied to measure the antioxidant activity of the coffee fractions. Fourier Transform Infrared- Attenuated Total Reflectance (FTIR-ATR) based chemometric approach was used to identify the compound functional groups as the fingerprinting profile of the coffee fractions. Correlation between the FTIR-ATR fingerprinting with the antioxidant activity of the coffee fractions was studied using multivariate data analysis, i.e. Principal Component Analysis (PCA) and Orthogonal Partial Least Squares (OPLS). From this study, a reliable PCA model to evaluate the effect of different solvents to FTIR-ATR fingerprinting profile was produced. The correlation between FTIR-ATR fingerprinting profile with the antioxidant activity and the characterization of the chemical functional groups relevant to its antioxidant activity can be analyzed by a reliable OPLS model obtained. This study suggests that the highest antioxidant potential in coffee is found in ethyl acetate fraction both in robusta and arabica coffee samples, while the relevant chemical functional groups having positive correlation to antioxidant activity of coffee were phenol, carbonyl, cyclohexane, aromatic, amide, phenyl, amino, and alkene groups.
咖啡富含抗氧化化合物,对健康有积极影响。咖啡的潜在抗氧化活性受到其化学成分的强烈影响。本研究旨在分析不同溶剂对咖啡化学代谢物谱和抗氧化活性的影响,并确定对咖啡抗氧化活性有积极作用的相关化学官能团。本研究采用四种极性溶剂对罗布斯塔和阿拉比卡两种咖啡样品的甲醇提取物进行液液分离。采用ABTS(2,2'-氮化氮(3-乙基苯并噻唑-6-磺酸))和cu还原抗氧化能力(CUPRAC)测定咖啡馏分的抗氧化活性。采用基于傅里叶变换红外衰减全反射(FTIR-ATR)的化学计量学方法鉴定了复合官能团作为咖啡馏分的指纹图谱。采用主成分分析(PCA)和正交偏最小二乘(ops)多元数据分析,研究了红外光谱- atr指纹图谱与咖啡提取物抗氧化活性的相关性。本研究建立了一个可靠的PCA模型来评估不同溶剂对FTIR-ATR指纹图谱的影响。FTIR-ATR指纹图谱与抗氧化活性的相关性以及与抗氧化活性相关的化学官能团的表征可以通过得到可靠的ops模型进行分析。本研究表明,罗布斯塔和阿拉比卡咖啡样品中的乙酸乙酯部分具有最高的抗氧化潜力,而与咖啡抗氧化活性正相关的化学官能团是苯酚、羰基、环己烷、芳香族、酰胺、苯基、氨基和烯基。
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引用次数: 0
The Influence of Xanthan Gum and Lemon Juice on the Quality of Tomato Sorbet 黄原胶和柠檬汁对番茄冰沙品质的影响
Pub Date : 2022-12-27 DOI: 10.6066/jtip.2022.33.2.148
P. Ramadhany, Gerry Irawan
Sorbet is one of common frozen desserts. It is prepared with low concentration of fat and protein; thus, the use of stabilizer in sorbet formulation extremely dictates the final properties. This current work investigated the quality (hardness, total solids, °Brix, viscosity, overrun, melting rate, vitamin C, lycopene content, and organoleptic test) of tomato-based sorbet added with different levels of xanthan gum as the stabilizer and lemon juice as the taste improver. The results showed that increase in xanthan gum level up to 0.5% was able to improve the overrun, melting rate, and lycopene content, i.e. 35%, 0.84 g/min, and 1.66 mg/100 g, respectively. Meanwhile, the addition of lemon juice into sorbet formulation could increase the content of vitamin C. Furthermore, addition of lemon juice was effective in removing the unpleasant tomato taste in the sorbet, but it did not affect the hardness, total solids, °Brix, lycopene content, viscosity, overrun, and melting rate.
冰糕是一种常见的冷冻甜点。它是用低浓度的脂肪和蛋白质制备的;因此,在冰糕配方中稳定剂的使用极大地决定了冰糕的最终性能。本文研究了添加不同水平黄原胶作为稳定剂和柠檬汁作为味道改进剂的番茄冰糕的质量(硬度、总固形物、白度、粘度、溢出、融化速度、维生素C、番茄红素含量和感官测试)。结果表明,添加0.5%的黄原胶可提高番茄红素含量,使番茄红素含量提高1.66 mg/100 g,溶化率提高35%,番茄红素含量提高0.84 g/min;同时,在冰糕配方中加入柠檬汁可以提高冰糕中维生素c的含量。此外,加入柠檬汁可以有效去除冰糕中令人不愉快的番茄味,但对冰糕的硬度、总固形物、°糖度、番茄红素含量、粘度、溢出和融化速度没有影响。
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引用次数: 1
Integrasi Metode Kano dan Turf dalam Evaluasi Sensori Minuman Cokelat Instan Komersial Kano和Turf方法在商业即时巧克力传感器评估中的集成
Pub Date : 2022-12-27 DOI: 10.6066/jtip.2022.33.2.137
Duantra Bergas Ari Kunto, Dase Hunaefi, Budi Nurtama
Sensory attribute is one of quality parameter that affects buying intention of consumer for commercial instant chocolate drink product. Evaluation of consumer liking using hedonic method or ideal profile method was not enough to fulfill consumer satisfaction. This research aims to identify sensory attributes which are able to give consumer satisfaction using survey method integrated with structural equation model (SEM), Kano method, and total unduplicated reach and frequency (TURF). Kano method is used to evaluate sensory attributes based on consumer satisfaction, while TURF is used to identify sensory component which is able to increase buying intention of consumers with regard to the range and frequency for chocolate instant commercial drink. Preliminary research conducted by modelling consumer preferences suggested that there was an influence of 0.76 sensory attributes to consumer satisfaction. Seven samples were purchased from market and evaluated by 30 untrained panelists using home use test with an approach of consumer habit to consume hot chocolate drink once a week. The panelists were chosen from people who consume commercial instant chocolate drink. Results of this research showed that sensory attributes creamy (texture) and dark chocolate (color) were attractive features which were able to improve consumer satisfaction. Atribute sweet (taste) was categorized as must-be features, while flavor, aroma, and color were classified as one-dimensional features. Combination of attributes in one category can improve the range to reach the targeted consumers and frequency of consumers towards the product, for example attribute creamy can reach 97%, but combination of attributes creamy and thickness can reach 100% of the targeted consumers.
感官属性是影响消费者对商品速溶巧克力饮料购买意愿的质量参数之一。用享乐法或理想剖面法评价消费者喜好不足以满足消费者满意度。本研究旨在利用结构方程模型(SEM)、卡诺法和总不可重复到达和频率(TURF)相结合的调查方法,确定能够给予消费者满意度的感官属性。以消费者满意度为基础,采用Kano法评价感官属性,采用TURF法识别感官成分,在巧克力速溶商业饮料的范围和频率上增加消费者的购买意愿。通过对消费者偏好进行建模的初步研究表明,有0.76的感官属性对消费者满意度的影响。从市场上购买7份样品,由30名未经培训的小组成员采用家庭使用测试的方法进行评估,采用消费者习惯每周饮用一次热巧克力饮料的方法。小组成员是从消费商业速溶巧克力饮料的人群中挑选出来的。这项研究的结果表明,奶油(质地)和黑巧克力(颜色)的感官属性是有吸引力的特征,能够提高消费者的满意度。属性甜(味道)被归类为必备特征,而风味、香气和颜色被归类为一维特征。一个品类的属性组合可以提高达到目标消费者的范围和消费者对产品的频率,例如属性奶油可以达到97%,而属性奶油和厚度的组合可以达到100%的目标消费者。
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引用次数: 0
Karakterisasi Fisiko-Kimia Biji dan Kulit Ari Kacang Bogor Asal Jampang-Sukabumi Jawa Barat 西爪哇省sukabumi的种子化学和皮肤茂物的特性
Pub Date : 2022-12-27 DOI: 10.6066/jtip.2022.33.2.178
R. Astuti, Nurheni Sri Palupi, Ma. Suhartono, Hanifah Nuryani Lioe, E. Kusumaningtyas, Laras Cempaka
The traditional cultivation and limited use of bambara groundnut (Vigna subterranea) seed and coat have encouraged the development of this commodity. The aim of this research was to characterize the seed and coat of bambara groundnut from Jampang, Kab. Sukabumi, West Java. Analysis on bambara groundnut seed including proximate analysis, in vitro protein digestibility, starch content, and dietary fiber, as well as analysis on its coat including anthocyanin, total phenolic, antioxidant activity, phytic acid and tannin, were examined in this study. The results showed that bambara groundnut seed from Jampang-Sukabumi contained 16.53% proteins, 3.04% ash, 7.83% fats and 55.22% carbohydrates in dry basis (db). The carbohydrates consisted of starch 52.71% and dietary fiber 7.47% (db). The protein had an in vitro protein digestibility of 41.65% db. The purple seed coat contained of 1.51% anthocyanin, 25.85 mg/g total phenolic content (as gallic acid equivalent), antioxidant activity at 82.75% inhibition of free radical DPPH, 6.37 mg/g phytic acid, and 96.79 mg/g tannin (as tannic acid equivalent) in dry basis. The relatively high content of tannin and antioxidant activity but very low phytic acid content, make the bambara seed coat a potential source for tannin, meanwhile the bambara groundnut is potential as a nutrition source.
传统的种植和有限使用的山核桃(Vigna subterranea)种子和外壳鼓励了这种商品的发展。本研究的目的是对来自Kab Jampang的班巴拉花生的种子和外壳进行表征。西爪哇苏加布米。本研究对巴马花生种子进行了分析,包括接近分析、蛋白质体外消化率、淀粉含量和膳食纤维,以及对其外壳花青素、总酚、抗氧化活性、植酸和单宁的分析。结果表明,在干基(db)中,来自Jampang Sukabumi的班巴拉花生种子含有16.53%的蛋白质、3.04%的灰分、7.83%的脂肪和55.22%的碳水化合物。碳水化合物由52.71%的淀粉和7.47%的膳食纤维组成。该蛋白的体外蛋白质消化率为41.65%db。紫色种皮中含有1.51%的花青素、25.85mg/g的总酚含量(以没食子酸当量计)、82.75%的自由基DPPH抑制抗氧化活性、6.37mg/g的植酸和96.79mg/g的单宁(以单宁酸当量计算)。其单宁含量和抗氧化活性相对较高,但植酸含量极低,使其种皮成为单宁的潜在来源,同时也使其花生成为潜在的营养来源。
{"title":"Karakterisasi Fisiko-Kimia Biji dan Kulit Ari Kacang Bogor Asal Jampang-Sukabumi Jawa Barat","authors":"R. Astuti, Nurheni Sri Palupi, Ma. Suhartono, Hanifah Nuryani Lioe, E. Kusumaningtyas, Laras Cempaka","doi":"10.6066/jtip.2022.33.2.178","DOIUrl":"https://doi.org/10.6066/jtip.2022.33.2.178","url":null,"abstract":"The traditional cultivation and limited use of bambara groundnut (Vigna subterranea) seed and coat have encouraged the development of this commodity. The aim of this research was to characterize the seed and coat of bambara groundnut from Jampang, Kab. Sukabumi, West Java. Analysis on bambara groundnut seed including proximate analysis, in vitro protein digestibility, starch content, and dietary fiber, as well as analysis on its coat including anthocyanin, total phenolic, antioxidant activity, phytic acid and tannin, were examined in this study. The results showed that bambara groundnut seed from Jampang-Sukabumi contained 16.53% proteins, 3.04% ash, 7.83% fats and 55.22% carbohydrates in dry basis (db). The carbohydrates consisted of starch 52.71% and dietary fiber 7.47% (db). The protein had an in vitro protein digestibility of 41.65% db. The purple seed coat contained of 1.51% anthocyanin, 25.85 mg/g total phenolic content (as gallic acid equivalent), antioxidant activity at 82.75% inhibition of free radical DPPH, 6.37 mg/g phytic acid, and 96.79 mg/g tannin (as tannic acid equivalent) in dry basis. The relatively high content of tannin and antioxidant activity but very low phytic acid content, make the bambara seed coat a potential source for tannin, meanwhile the bambara groundnut is potential as a nutrition source.","PeriodicalId":17790,"journal":{"name":"Jurnal Teknologi dan Industri Pangan","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-12-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48348282","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Penurunan Indeks Glikemik Nasi Putih dengan Penambahan Ekstrak Serai dan Daun Salam 血清提取物和萨拉姆片降低白鼻血糖指数
Pub Date : 2022-12-27 DOI: 10.6066/jtip.2022.33.2.169
Fairuz Fajriah, D. Faridah, Dian Herawati
Diabetes mellitus (DM) is one of the non-communicable diseases that could be prevented by consumption of foods capable of maintaining blood glucose at a safe level. Phenolic compounds are  components in food that affect blood glucose levels. Lemongrass and bay leaf are Indonesian spices commonly used for cooking and contain phenolic compounds that have potential as antidiabetic compounds. The aim of this study was to evaluate the effect of adding lemongrass and bay leaves water extracts on the GI value of cooked white rice. Lemongrass and bay leaves containing phenolic compounds were extracted with water and added to white rice during the cooking process or sprayed on cooked rice. The glycemic index of the tested food measured using the ISO 26642 method showed that the addition of lemongrass extract and a combination of lemongrass and bay leaf extract with total phenolic content (TPC) of 570 mg GAE/100 g and 565 mg GAE/100 g, respectively, on cooked white rice IR 64 resulted in the GI reduction in the cooked rice by 23 and 27%, respectively. These reduction was higher than those resulted from the addition of lemon grass or the combination of lemon grass and bay leaf extract during the cooking process, i.e. 9 and 13%, respectively.
糖尿病(DM)是一种非传染性疾病,可以通过食用能够将血糖维持在安全水平的食物来预防。酚类化合物是食物中影响血糖水平的成分。柠檬草和月桂叶是印度尼西亚的香料,通常用于烹饪,含有酚类化合物,有潜在的抗糖尿病化合物。本研究的目的是评价添加香茅和月桂叶水提取物对煮熟白米GI值的影响。用水提取含有酚类化合物的香茅和月桂叶,在蒸煮过程中加入到白米饭中,或喷洒在煮熟的米饭上。采用ISO 26642方法测定了被试食品的升糖指数,结果表明,在熟白米IR 64中分别添加总酚含量(TPC)为570 mg GAE/100 g和565 mg GAE/100 g的香茅提取物和香茅与月桂叶提取物组合,可使熟白米的升糖指数分别降低23%和27%。这比在蒸煮过程中添加柠檬草或柠檬草与月桂叶提取物组合的效果要高,分别为9%和13%。
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引用次数: 0
Ready-To-Eat Rice in Retort Pouch Packaging as an Alternative Emergency Food Product 以蒸煮袋包装即食大米作为应急食品的替代方案
Pub Date : 2022-12-27 DOI: 10.6066/jtip.2022.33.2.129
S. Rahayu, T. Muhandri, D. Hunaefi, P. Fuhrmann, I. Smetanska
Recently, the rising incidence of natural catastrophe has increased the disaster preparedness, aiming to mitigate its devastating effect. Developing an emergency food is one of meaningful attempts to rise the preparedness. This research aimed to determine the best formula of ready-to-eat rice in retort pouch packaging accepted by consumers, and to determine the operating time to reach a lethality value (F0) to meet the commercial shelf-stable food requirements as an emergency food. The thermal process adequacy (F0) was used to determine the commercial shelf-stable products according to Indonesian regulation. The results showed that the determination of ready-to-eat rice was dependent on the ratio of rice and water. The most accepted product was determined according to quality attributes and organoleptic tests applied to meet the criteria for emergency food, namely color, flavor, and texture and best perceived by consumers. A formula with 140 g of half-cooked rice and 60 g of water was attributed to the best sample, having hardness of 7305.45 gf, elasticity of 36.40%, gumminess of 2185.720 gf, and adhesiveness of -167.975 g.s. In terms of microbiological quality, the TPC for the half-cooked rice sample reached 7.2×107 CFU/mL, while cooked rice in retort pouch packaging was <25 CFU/mL. Using heat distribution curve, heating at 110°C produced a come up time (CUT) after 40 min. Furthermore, the F0 value was 4.12 which was in accordance with the Indonesian regulation.
近年来,随着自然灾害发生率的上升,防灾工作日益加强,旨在减轻其破坏性影响。开发应急食品是提高应急准备的有意义的尝试之一。本研究旨在确定消费者可接受的蒸煮袋装即食大米的最佳配方,并确定其作为应急食品达到致死值(F0)的操作时间,以满足商业货架稳定性食品的要求。根据印尼法规,采用热过程充分性(F0)来确定商业货架稳定性产品。结果表明,即食大米的测定依赖于大米与水的比例。最可接受的产品是根据质量属性和感官测试来确定的,这些测试是为了满足紧急食品的标准,即颜色、味道和质地,并为消费者所感知。以140 g半熟米加60 g水的配方为最佳样品,其硬度为7305.45 gf,弹性为36.40%,胶度为2185.720 gf,黏附力为-167.975 g.s。在微生物质量方面,半熟米样品的TPC达到7.2×107 CFU/mL,而蒸煮袋包装的熟米TPC <25 CFU/mL。根据热分布曲线,在110℃下加热40 min后产生了一个come up time (CUT), F0值为4.12,符合印尼规定。
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引用次数: 1
Produksi Hidrolisat Protein Kacang Koro Benguk dengan Aktivitas Penghambat Kerja Enzim Pengkonversi Angiotensin melalui Kombinasi Fermentasi dan Hidrolisis Enzimatik 球茎坚果的蛋白质含水率的生产,以及通过发酵和酶水合物的结合来抑制血管上情酶的活性
Pub Date : 2022-12-27 DOI: 10.6066/jtip.2022.33.2.157
Tesy Pratami, A. B. Sitanggang, C. H. Wijaya
Mucuna bean (Mucuna pruriens L.) is a legume having high protein content which has the potential as a source of bioactive peptides. One of the bioactive peptides is an angiotensin-converting enzyme (ACE) inhibitor, thus, mucuna beans might be used as a potential source of antihypertensive compounds. This study aimed to increase the functionality of proteins from mucuna beans as ACE inhibitors using a combination of fermentation and enzymatic hydrolysis followed by membrane filtration. The mucuna beans were fermented for 0, 24, 48, 96, and 144 h. The highest ACE inhibitory activity of 54.37%, was obtained by fermentation of the beans at 48 h, with a protein content of 20.82 mg/mL. The 48 h fermented mucuna beans were further hydrolyzed using alcalase or neutrase and subsequently filtered with UF membranes having 20,10 and 5 kDa cut-off. The enzymatic hydrolysis followed by membrane filtration increased the ACE inhibitory activity of mucuna beans. The neutrase hydrolysates resulting from 5 kDa membrane filtration showed the best ACE inhibitory activity (62.96% with a protein content of 10.39 mg/mL). A combination of fermentation and enzymatic hydrolysis followed by filtration using UF-membrane was able to produce ACE inhibitory peptides from mucuna beans. The potential of mucuna beans peptides as ACE inhibitors was due to the presence of negatively charged amino acid residues such as Asp and Glu, positively charged amino acids such as Arg and Lys, and hydrophobic amino acids such as Val, Leu, Ala, and Ile.
粘豆(Mucuna pruriens L.)是一种蛋白质含量高的豆科植物,具有作为生物活性肽来源的潜力。其中一种生物活性肽是血管紧张素转换酶(ACE)抑制剂,因此,黏液豆可能被用作抗高血压化合物的潜在来源。本研究旨在利用发酵、酶解和膜过滤相结合的方法提高蚕豆蛋白作为ACE抑制剂的功能。分别发酵0、24、48、96和144 h,发酵48 h时ACE抑制活性最高,为54.37%,蛋白含量为20.82 mg/mL。将发酵48 h的豆荚用碱性酶或中和酶进一步水解,然后用截断20kda、10kda和5kda的UF膜过滤。酶解后再进行膜过滤,可提高豆豆的ACE抑制活性。5 kDa膜过滤得到的中和酶水解产物ACE抑制活性最高,为62.96%,蛋白含量为10.39 mg/mL。通过发酵和酶解结合,然后用uf膜过滤,可以从蚕豆中提取ACE抑制肽。蚕豆肽作为ACE抑制剂的潜力是由于存在带负电荷的氨基酸残基,如Asp和Glu,带正电荷的氨基酸,如Arg和Lys,以及疏水氨基酸,如Val, Leu, Ala和Ile。
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引用次数: 0
Characterization of Chemical Properties of Inulin Isolated from Yacón Tuber Yacón Tuber菊粉化学性质的表征
Pub Date : 2022-12-27 DOI: 10.6066/jtip.2022.33.2.111
D. Andrianto, M. Bintang, Irfan Aziz, Suci Hermita
Inulin is a polysaccharide with linear fructan chain structure. Food industries widely use inulin as a low calorie sugar substitutions. Yacón (Smallanthus sonchifolius) tuber that are easily grown and contain higher inulin can be explored as local inulin sources. This research aimed to characterize the chemical properties of inulin isolated from yacón tuber. The methods of this study include proximate analysis of yacón tuber, inulin isolation, and characterization of purified inulin using FTIR. The results showed that yacón consisted  of 91.23 water (analyzed as moisture), 0.12 proteins, 0.58 fats, 0.52 crude fibers, and 7.34% carbohydrates. The yield of inulin extracted from yacón tuber was 4.86% whereas its purity and actual content of inulin were 44.23 and 2.15%, respectively. The Osazon test revealed that the isolated inulin has similar crystalline with that from chicory. Based on characterization using the FTIR spectrophotometer, the isolated inulin had functional groups of C-O, C-H, CH2, O-H, and C=O. The spectrum of the isolated inulin has similarities with chicory inulin. Therefore, yacón tuber could be considered as a potential local inulin source in Indonesia.
菊粉是一种具有线性果聚糖链结构的多糖。食品工业广泛使用菊粉作为低热量糖的替代品。Yacón(Smallanthus sonchifolis)块茎易于种植,含有较高的菊粉,可以作为当地菊粉来源进行探索。本研究的目的是表征从亚康块茎中分离的菊粉的化学性质。本研究的方法包括对yacón块茎的近似分析、菊粉的分离以及使用FTIR对纯化菊粉的表征。结果表明,yacón由91.23水(按水分分析)、0.12蛋白质、0.58脂肪、0.52粗纤维和7.34%碳水化合物组成。从yacón块茎中提取的菊粉的产率为4.86%,而其纯度和菊粉的实际含量分别为44.23%和2.15%。Osazon试验表明,分离得到的菊粉具有与菊苣相似的结晶性。基于FTIR分光光度计的表征,分离的菊粉具有C-O、C-H、CH2、O-H和C=O官能团。分离的菊粉的光谱与菊苣菊粉有相似之处。因此,yacón块茎可以被认为是印度尼西亚当地菊粉的潜在来源。
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引用次数: 0
Potensi Probiotik Bakteri Asam Laktat Asal Madu dari Tiga Jenis Lebah yang Berbeda 三种不同肝脏蜂蜜酸细菌的益生菌潜力
Pub Date : 2022-12-27 DOI: 10.6066/jtip.2022.33.2.189
Iffa Illiyya Fatma, Lilis Nuraida, D. Faridah
Certain strains of Lactic acid bacteria (LAB) especially from the genus of Lactobacillus and Bifidobacteria have been recognized to have health beneficial effect as probiotics. Honey has been known to have health beneficial effects and contains lactic acid bacteria. However, information pertaining the characteristics of LAB from honey is still limited. The present research aimed to isolate LAB from different types of honey and to evaluate their potency as probiotic. The LAB were enumerated and isolated from honey produced by three different honeybees: Apis cerana, Heterotrigona itama, and Trigona laeviceps. The results showed the count of LAB in three different honey ranged from 5.0x101 to 2.3x107 CFU/mL and affected by different time of sampling. The highest of average LAB count was found in honey of Heterotrigona itama. There were 48 Gram positive catalase-negative bacterial isolates obtained from the three different honey types. Twelve isolates were selected based on their survival in bile salt. The twelve selected isolates were capable of growing in MRSB pH 2.5, and MRSB containing 0.3% bile salt. They also exhibited strong antibacterial activity against pathogenic bacteria. Identification based on 16S rRNA revealed that of the twelve isolates, nine were identified as Lactiplantibacillus plantarum and three others as Pediococcus acidilactici. The twelve isolates showed high survival at low pH dan bile salt and exhibited antimicrobial activity against pathogen, hence they are considered as probiotic candidates.
某些乳酸菌,特别是乳杆菌属和双歧杆菌属的乳酸菌已被认为是有益健康的益生菌。众所周知,蜂蜜对健康有益,并且含有乳酸菌。然而,有关蜂蜜中乳酸菌特性的信息仍然有限。本研究旨在从不同类型的蜂蜜中分离乳酸菌,并评价其作为益生菌的效力。从三种蜜蜂的蜂蜜中分别分离出了乳酸菌,分别是蜜蜂、黑斑异蜂和蓝蜂。结果表明,三种蜂蜜中乳酸菌的计数范围为5.0 × 101 ~ 2.3 × 107 CFU/mL,且受采样时间的影响。乳酸菌平均计数以黑麦蜜最高。从三种不同类型的蜂蜜中分离出48株革兰氏阳性过氧化氢酶阴性细菌。根据其在胆盐中的存活率选择了12株分离株。所选的12株菌株能够在MRSB pH为2.5和含有0.3%胆汁盐的MRSB中生长。对病原菌具有较强的抑菌活性。经16S rRNA鉴定,12株分离物中9株为植物乳酸杆菌,3株为酸碱Pediococcus acidilactii。这12株菌株在低pH胆盐环境下均表现出较高的存活率和抗菌活性,因此它们被认为是益生菌的候选菌。
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Jurnal Teknologi dan Industri Pangan
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