Pub Date : 2021-11-30DOI: 10.14334/jitv.v26i3.2945
Vida Safaei, M. Shariati
Sertraline is an antidepressant which has toxic effects on the liver. This study was conducted to evaluate the effect of Sertraline administration in pregnancy on liver function of male neonates of rats. Twenty-five pregnant female Wistar rats were divided into 4 groups of 5. The control group did not receive any drug treatments, but experimental (Exp) groups 1, 2 and 3 received 5, 10 and 20 mg/kg Sertraline as gavage throughout the pregnancy, respectively. Twenty-two days after birth, male rats were divided into 4 groups of 10 based on the previous division and after weighing, by taking blood directly from the heart, serum levels of Alanine transaminase (Alt), Aspartate transaminase (AST), Alkaline phosphatase (Alp), Albumin (Alb), Total protein (TP), and Bilirubin (Bili) were measured and the liver tissue was also analyzed histopathologically after weighing. In Exp groups, a significant decrease in body weight, TP and Alb serum levels were observed compared to the control group (p<0.05). In Exp group 3, a significant decrease in liver weight was observed compared to the control group (p˂0.05). In Exp groups 2 and 3, a significant increase in serum levels of Alp, Alt and Bili in was observed compared to the control group (p˂0.05). A significant increase in AST serum level was observed in Exp groups compared to the control group (p˂0.05). Liver tissue destruction was observed in all 3 Exp groups. The administration of Sertraline in pregnant female rats causes liver damage and increases liver enzymes and blood biochemical parameters in their male offspring.
{"title":"Studying the Liver Function in Male Neonates of Rats Born to Sertraline-Treated Mothers","authors":"Vida Safaei, M. Shariati","doi":"10.14334/jitv.v26i3.2945","DOIUrl":"https://doi.org/10.14334/jitv.v26i3.2945","url":null,"abstract":"Sertraline is an antidepressant which has toxic effects on the liver. This study was conducted to evaluate the effect of Sertraline administration in pregnancy on liver function of male neonates of rats. Twenty-five pregnant female Wistar rats were divided into 4 groups of 5. The control group did not receive any drug treatments, but experimental (Exp) groups 1, 2 and 3 received 5, 10 and 20 mg/kg Sertraline as gavage throughout the pregnancy, respectively. Twenty-two days after birth, male rats were divided into 4 groups of 10 based on the previous division and after weighing, by taking blood directly from the heart, serum levels of Alanine transaminase (Alt), Aspartate transaminase (AST), Alkaline phosphatase (Alp), Albumin (Alb), Total protein (TP), and Bilirubin (Bili) were measured and the liver tissue was also analyzed histopathologically after weighing. In Exp groups, a significant decrease in body weight, TP and Alb serum levels were observed compared to the control group (p<0.05). In Exp group 3, a significant decrease in liver weight was observed compared to the control group (p˂0.05). In Exp groups 2 and 3, a significant increase in serum levels of Alp, Alt and Bili in was observed compared to the control group (p˂0.05). A significant increase in AST serum level was observed in Exp groups compared to the control group (p˂0.05). Liver tissue destruction was observed in all 3 Exp groups. The administration of Sertraline in pregnant female rats causes liver damage and increases liver enzymes and blood biochemical parameters in their male offspring.","PeriodicalId":17806,"journal":{"name":"Jurnal Ilmu Ternak dan Veteriner","volume":" ","pages":""},"PeriodicalIF":0.5,"publicationDate":"2021-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46841750","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-11-30DOI: 10.14334/jitv.v26i3.2829
Merryafinola Ifani, E. A. Rimbawanto, F. M. Suhartati
The study was aimed to examine effect of protecting soybean meal using mahogany leaf extract on rumen fermentation products in vitro. The material used was cow rumen fluid, basal ration consisting of concentrate and elephant grass with a ratio of 60:40%, and mahogany leaves. The research was carried out in three stages: mahogany extraction, protein protection using mahogany extract, and in vitro stages. The test was conducted in vitro based on a completely randomized design (CRD). The treatments consisted of 4 kinds of soybean meal protection with 0% tannin concentration (T0); 1.5% (T1); 3% (T2); and 4.5% (T3). Data obtained were analyzed by analysis of variance and tested using orthogonal polynomials. Results showed that addition of protected soybean meal with mahogany leaf extract had a cubical effect on partial VFA, methane gas and post-rumen dissolved protein, a quadratic effect on protozoa, and a linear effecton N-NH3, SPM, and RUDP. Giving extra mahogany leaves at a level of 1.5% produced a fermented product that was not different from the control while giving a level of 3% got the highest fermentation product. Giving mahogany leaf extract at a level of 4.5% resulted in the highest SPM, and RUDP but there was a decrease in soluble protein, which indicated the occurrence of overprotection. The addition of 3% mahogany leaf extract effectively increased rumen fermentation products, RUDP, and soluble protein without disturbing the activity of rumen bacteria.
{"title":"Effect of Protection of Soybean Meal Using Mahogany Leaf Extract in Ruminant Diet on Rumen Fermentation Products","authors":"Merryafinola Ifani, E. A. Rimbawanto, F. M. Suhartati","doi":"10.14334/jitv.v26i3.2829","DOIUrl":"https://doi.org/10.14334/jitv.v26i3.2829","url":null,"abstract":"<p class=\"abstrak3\"><span lang=\"IN\">The study </span><span>was </span><span lang=\"IN\">aimed to examine effect of protecting soybean meal using mahogany leaf extract on rumen fermentation products in vitro. The material used was cow rumen fluid, basal ration consisting of concentrate and elephant grass with a ratio of 60:40%, and mahogany leaves. The research was carried out in three stages: mahogany extraction, protein protection using mahogany extract, and in vitro stages. The test was conducted in vitro </span><span>based on</span><span lang=\"IN\"> a completely randomized design (CRD). The treatments consisted of 4 kinds of soybean meal protection with 0% tannin concentration (</span><span>T</span><span lang=\"IN\">0); 1.5% (</span><span>T</span><span lang=\"IN\">1); 3% (</span><span>T</span><span lang=\"IN\">2); and 4.5% (</span><span>T</span><span lang=\"IN\">3). </span><span>D</span><span lang=\"IN\">ata obtained were analyzed by analysis of variance and tested using orthogonal polynomials. </span><span>R</span><span lang=\"IN\">esults showed that addition of protected soybean meal with mahogany leaf extract had a cubic</span><span>al</span><span lang=\"IN\"> effect on partial VFA, methane gas and post-rumen dissolved protein, a quadratic effect on protozoa, and a linear effect</span><span>on </span><span lang=\"IN\">N-NH<sub>3</sub>, SPM, and RUDP. Giving extra mahogany leaves at a level of 1.5% produced a fermented product that was not different from the control while giving a level of 3% got the highest fermentation product. Giving mahogany leaf extract at a level of 4.5% resulted in the highest SPM, and RUDP but there was a decrease in soluble protein, which indicated the occurrence of overprotection. The addition of 3% mahogany leaf extract effectively increased rumen fermentation products, RUDP, and soluble protein without disturbing the activity of rumen bacteria.</span></p>","PeriodicalId":17806,"journal":{"name":"Jurnal Ilmu Ternak dan Veteriner","volume":" ","pages":""},"PeriodicalIF":0.5,"publicationDate":"2021-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44643822","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-11-30DOI: 10.14334/jitv.v26i3.2851
H. Hasbi, H. Sonjaya, S. Baco, Reski Amalia, S. Gustina
Bali cattle are one of the original Indonesian germplasm, which in its development were found to be hornless or polled. Polled are Balinese cattle whose horns do not grow naturally, but have the same characteristics as those with horns. However, there are indications that polled have a lower libido. The purpose of this study was to determine the characteristics of libido and testosterone concentration after gonadotrophin releasing hormone (GnRH) injection. In this study each of 7 male polled and horned Bali cattle aged 3.5-6 years were used. Libido characteristics were observed by recording the first time the male fondled the teaser until ejaculation, while the testosterone concentration was measured using blood plasma taken on day 0 or before injection, day 7th, and day 14th after GnRH injection. Testosterone analysis used enzyme linked immunosorbent assay (ELISA) method. The results showed that the libido of polled was not different (P>0.05) compared to that of the horned on the 0, 7th and 14th days after GnRH injection. However, in polled on the 7th day after GnRH injection, it was significantly lower than on day 0 and 14, but on day 0 it was not different with day 14. Testosterone concentration on day 7 after injection of GnRH was not different (P>0.05) compared to day 0 both in polled and horned bulls, but on day 14 after injection was significantly higher (P<0.05) in horned than polled. In conclusion, polled and horned Bali bulls had high libido with a score of +1, but on the 7th day after GnRH injection, polled had lower libido than horns. The testosterone concentrations of polled and horned on day 7 after GnRH injection were not different compared to day 0, while the 14th day was higher in horned bull.
{"title":"Characteristics of Libido and Testosterone Concentration of Polled and Horned Bali Bulls after GnRH Injection","authors":"H. Hasbi, H. Sonjaya, S. Baco, Reski Amalia, S. Gustina","doi":"10.14334/jitv.v26i3.2851","DOIUrl":"https://doi.org/10.14334/jitv.v26i3.2851","url":null,"abstract":"Bali cattle are one of the original Indonesian germplasm, which in its development were found to be hornless or polled. Polled are Balinese cattle whose horns do not grow naturally, but have the same characteristics as those with horns. However, there are indications that polled have a lower libido. The purpose of this study was to determine the characteristics of libido and testosterone concentration after gonadotrophin releasing hormone (GnRH) injection. In this study each of 7 male polled and horned Bali cattle aged 3.5-6 years were used. Libido characteristics were observed by recording the first time the male fondled the teaser until ejaculation, while the testosterone concentration was measured using blood plasma taken on day 0 or before injection, day 7th, and day 14th after GnRH injection. Testosterone analysis used enzyme linked immunosorbent assay (ELISA) method. The results showed that the libido of polled was not different (P>0.05) compared to that of the horned on the 0, 7th and 14th days after GnRH injection. However, in polled on the 7th day after GnRH injection, it was significantly lower than on day 0 and 14, but on day 0 it was not different with day 14. Testosterone concentration on day 7 after injection of GnRH was not different (P>0.05) compared to day 0 both in polled and horned bulls, but on day 14 after injection was significantly higher (P<0.05) in horned than polled. In conclusion, polled and horned Bali bulls had high libido with a score of +1, but on the 7th day after GnRH injection, polled had lower libido than horns. The testosterone concentrations of polled and horned on day 7 after GnRH injection were not different compared to day 0, while the 14th day was higher in horned bull.","PeriodicalId":17806,"journal":{"name":"Jurnal Ilmu Ternak dan Veteriner","volume":" ","pages":""},"PeriodicalIF":0.5,"publicationDate":"2021-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49163752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-09-30DOI: 10.14334/JITV.V26I3.2758
Ikhsan Suhendro, J. Jakaria, R. Priyanto, W. Manalu, R. R. Noor
Bali cattle (Bos javanicus) are common species distributed throughout Indonesia to survive in tropical hot, and humid climates. Fluctuating asymmetry (FA) is a random deviation from perfect bilateral symmetry. FA is often used to measure developmental stability in individuals and can be used as a marker for the impact of environmental and genetic stress. This study aims to investigate the pattern of developmental instability in Bali cattle as caused by environmental stress at various altitudes using the FA index. FA indexes in this study were used to compare asymmetrical physical traits of Bali cattle in Sembalun high altitude and Serading low altitude. Sixty-five Bali cattle were used in this study reared at different altitudes, namely Serading, Sumbawa Island (50 m above sea level), and Sembalun, Lombok Island (1,186 m above sea level). The physical traits of Bali cattle measured were horn circumference (horn), a distance of hip to spine bone (pelvic), metatarsal circumference (metatarsal), and metacarpal circumference (metacarpal). The FA1 and FA5 indexes showed significant metacarpal differences between Bali cattle reared at Sembalun and Serading population (P<0.05). FA11 multiple trait index as a composite of all traits revealed a significant difference as well. (P<001). It can be concluded that various heat stress by altitude impacts the asymmetry of Bali cattle.
{"title":"Fluctuating Asymmetry Increases with Heat Stress Disruptions on Bali Cattle (Bos javanicus) at Different Altitude","authors":"Ikhsan Suhendro, J. Jakaria, R. Priyanto, W. Manalu, R. R. Noor","doi":"10.14334/JITV.V26I3.2758","DOIUrl":"https://doi.org/10.14334/JITV.V26I3.2758","url":null,"abstract":"Bali cattle (Bos javanicus) are common species distributed throughout Indonesia to survive in tropical hot, and humid climates. Fluctuating asymmetry (FA) is a random deviation from perfect bilateral symmetry. FA is often used to measure developmental stability in individuals and can be used as a marker for the impact of environmental and genetic stress. This study aims to investigate the pattern of developmental instability in Bali cattle as caused by environmental stress at various altitudes using the FA index. FA indexes in this study were used to compare asymmetrical physical traits of Bali cattle in Sembalun high altitude and Serading low altitude. Sixty-five Bali cattle were used in this study reared at different altitudes, namely Serading, Sumbawa Island (50 m above sea level), and Sembalun, Lombok Island (1,186 m above sea level). The physical traits of Bali cattle measured were horn circumference (horn), a distance of hip to spine bone (pelvic), metatarsal circumference (metatarsal), and metacarpal circumference (metacarpal). The FA1 and FA5 indexes showed significant metacarpal differences between Bali cattle reared at Sembalun and Serading population (P<0.05). FA11 multiple trait index as a composite of all traits revealed a significant difference as well. (P<001). It can be concluded that various heat stress by altitude impacts the asymmetry of Bali cattle.","PeriodicalId":17806,"journal":{"name":"Jurnal Ilmu Ternak dan Veteriner","volume":" ","pages":""},"PeriodicalIF":0.5,"publicationDate":"2021-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47539927","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-07-07DOI: 10.14334/JITV.V26I2.2715
Masrurah Masrurah, I. Khaerunnisa, S. Murtini, C. Sumantri
Avian Beta Defensin 2 (AvBD2) gene, which is located in chromosome 3, plays an important role in the immune system of the chicken by inhibiting the development of microorganisms such as bacteria that infect body tissues. Defensins are produced through epithelial cells immediately after tissue injury or infection, which then processes the maturation of dendritic cells to initiate an immune response in the lymph nodes. The purpose of this study was to discover the polymorphism of the AvBD2 gene in IPB-D1 chickens. PCR and direct-DNA sequencing methods were used to identify the diversity of intron 1, exon 2, and intron 2 AvDB2 genes in 47 chickens. Genotype and allele frequency, and heterozygosity calculations were carried out to obtain information of the AvBD2 gene polymorphism. A total of 10 single nucleotide polymorphisms were found in the AvBD2 gene located in intron 1 (g.4843T>A, g.4853G>A, and g.4859T>C), exon 2 (g.4881A>G, g.4889G>A, and g.5002C>T), and intron 2 (g.5075C>T, g.5111T>G, g.5116G>T, and g.5177G>T). All SNPs are polymorphic. The g.5002C>T mutation causes changes in the amino acid Ala to Val which has the potential to be a candidate for characterizing disease resistance in IPB-D1 chickens.
{"title":"Avian Beta Defensin 2 (AvBD2) Gene Polymorphism Identification in IPB-D1 Chicken","authors":"Masrurah Masrurah, I. Khaerunnisa, S. Murtini, C. Sumantri","doi":"10.14334/JITV.V26I2.2715","DOIUrl":"https://doi.org/10.14334/JITV.V26I2.2715","url":null,"abstract":"Avian Beta Defensin 2 (AvBD2) gene, which is located in chromosome 3, plays an important role in the immune system of the chicken by inhibiting the development of microorganisms such as bacteria that infect body tissues. Defensins are produced through epithelial cells immediately after tissue injury or infection, which then processes the maturation of dendritic cells to initiate an immune response in the lymph nodes. The purpose of this study was to discover the polymorphism of the AvBD2 gene in IPB-D1 chickens. PCR and direct-DNA sequencing methods were used to identify the diversity of intron 1, exon 2, and intron 2 AvDB2 genes in 47 chickens. Genotype and allele frequency, and heterozygosity calculations were carried out to obtain information of the AvBD2 gene polymorphism. A total of 10 single nucleotide polymorphisms were found in the AvBD2 gene located in intron 1 (g.4843T>A, g.4853G>A, and g.4859T>C), exon 2 (g.4881A>G, g.4889G>A, and g.5002C>T), and intron 2 (g.5075C>T, g.5111T>G, g.5116G>T, and g.5177G>T). All SNPs are polymorphic. The g.5002C>T mutation causes changes in the amino acid Ala to Val which has the potential to be a candidate for characterizing disease resistance in IPB-D1 chickens.","PeriodicalId":17806,"journal":{"name":"Jurnal Ilmu Ternak dan Veteriner","volume":" ","pages":""},"PeriodicalIF":0.5,"publicationDate":"2021-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42808491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-06-29DOI: 10.14334/jitv.v26i2.2701
D. Dairoh, J. Jakaria, M. F. Ulum, A. Ishak, C. Sumantri
Bali cattle (Bos javanicus) are native Indonesian cattle, domesticated from banteng (Bibos banteng). Genes that have an important role in meat quality are calcium-activated neutral protease genes, known as calpains (CAPN). The objective of this study was to evaluate the polymophisms of calpain gene SNP g.232 G>T by PCR-RFLP technique and its influence on growth trait and meat quality of Bali cattle detected by ultrasound imaging of longissimus dorsi thickness (LDT), back fat thickness (BFT), marbling score (MS), and intramuscular fat percentage (PIMF). The polymorphisms of CAPN1 gene were analyzed by PCR-RFLP using BglII restriction enzyme (n=52 cattle). The ultrasound images of longissimus dorsi muscle were carried out transversally and longitudinal between 12th -13th thoracic vertebrae then analyzed by Image-J NIH software. Result showed that SNP g.232 G>T of CAPN1 gene was polymorphic in Bali cattle. SNP g.232 G>T of CAPN1 gene in Bali cattle has higher diversity which was showed of 0.48 heterozygosity value and was in Hardy-Weinberg equilibrium. The polymorphisms of SNP g.232 G>T was associated significantly (P<0.05) with bodyweight at 730 days, marbling score (MS), and intramuscular fat percentage (PIMF). It suggests that the CAPN1 gene in Bali cattle is a candidate for Marker Assisted Selection (MAS), which influences body weight at 730 days, marbling score, and percentage of intramuscular fat.
{"title":"Association of SNP g.232 G>T Calpain Gene with Growth and Live Meat Quality Prediction using Ultrasound Images in Bali Cattle","authors":"D. Dairoh, J. Jakaria, M. F. Ulum, A. Ishak, C. Sumantri","doi":"10.14334/jitv.v26i2.2701","DOIUrl":"https://doi.org/10.14334/jitv.v26i2.2701","url":null,"abstract":"Bali cattle (Bos javanicus) are native Indonesian cattle, domesticated from banteng (Bibos banteng). Genes that have an important role in meat quality are calcium-activated neutral protease genes, known as calpains (CAPN). The objective of this study was to evaluate the polymophisms of calpain gene SNP g.232 G>T by PCR-RFLP technique and its influence on growth trait and meat quality of Bali cattle detected by ultrasound imaging of longissimus dorsi thickness (LDT), back fat thickness (BFT), marbling score (MS), and intramuscular fat percentage (PIMF). The polymorphisms of CAPN1 gene were analyzed by PCR-RFLP using BglII restriction enzyme (n=52 cattle). The ultrasound images of longissimus dorsi muscle were carried out transversally and longitudinal between 12th -13th thoracic vertebrae then analyzed by Image-J NIH software. Result showed that SNP g.232 G>T of CAPN1 gene was polymorphic in Bali cattle. SNP g.232 G>T of CAPN1 gene in Bali cattle has higher diversity which was showed of 0.48 heterozygosity value and was in Hardy-Weinberg equilibrium. The polymorphisms of SNP g.232 G>T was associated significantly (P<0.05) with bodyweight at 730 days, marbling score (MS), and intramuscular fat percentage (PIMF). It suggests that the CAPN1 gene in Bali cattle is a candidate for Marker Assisted Selection (MAS), which influences body weight at 730 days, marbling score, and percentage of intramuscular fat.","PeriodicalId":17806,"journal":{"name":"Jurnal Ilmu Ternak dan Veteriner","volume":" ","pages":""},"PeriodicalIF":0.5,"publicationDate":"2021-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45785373","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-06-29DOI: 10.14334/jitv.v26i2.2729
K. Buranaamnuay, A. Kettawan, C. Changsangfa, S. Aiemongkot
Calcium is one of the minerals that are essential for male reproductive function. Calcium deficiency adversely affects spermatogenesis, normal sperm function and results in infertility. The sperm quality of rats fed a standard diet containing chicken bone extract powder (BEP) was assessed in the present study. Twenty male 8-week-old rats, Wistar strain, were randomized by weight into two groups of ten rats each and fed ad libitum a standard diet containing calcium carbonate (CaCO3, control) or chicken BEP; both were equivalent to 0.5% calcium. At the end of the 7-week consumption, the net body weight gains measured in control (101.33±21.81 g) and chicken BEP groups (100.74±26.80 g) were not significantly different (P>0.05). The in vitro sperm quality in terms of concentration, motility, viability, resistance to hypotonic stress, acrosomal reaction ability and morphology was comparable between control and chicken BEP (all were P>0.05). The results suggest that chicken BEP addition into feeds is an alternative calcium source that is as effective but less expensive as CaCO3, a commercial calcium (fortificant). At least, it has no detrimental effect on male reproductive function.
{"title":"Effect of Chicken Bone Extract Powder on Epididymal Sperm Quality of Male Wistar Rats","authors":"K. Buranaamnuay, A. Kettawan, C. Changsangfa, S. Aiemongkot","doi":"10.14334/jitv.v26i2.2729","DOIUrl":"https://doi.org/10.14334/jitv.v26i2.2729","url":null,"abstract":"Calcium is one of the minerals that are essential for male reproductive function. Calcium deficiency adversely affects spermatogenesis, normal sperm function and results in infertility. The sperm quality of rats fed a standard diet containing chicken bone extract powder (BEP) was assessed in the present study. Twenty male 8-week-old rats, Wistar strain, were randomized by weight into two groups of ten rats each and fed ad libitum a standard diet containing calcium carbonate (CaCO3, control) or chicken BEP; both were equivalent to 0.5% calcium. At the end of the 7-week consumption, the net body weight gains measured in control (101.33±21.81 g) and chicken BEP groups (100.74±26.80 g) were not significantly different (P>0.05). The in vitro sperm quality in terms of concentration, motility, viability, resistance to hypotonic stress, acrosomal reaction ability and morphology was comparable between control and chicken BEP (all were P>0.05). The results suggest that chicken BEP addition into feeds is an alternative calcium source that is as effective but less expensive as CaCO3, a commercial calcium (fortificant). At least, it has no detrimental effect on male reproductive function.","PeriodicalId":17806,"journal":{"name":"Jurnal Ilmu Ternak dan Veteriner","volume":" ","pages":""},"PeriodicalIF":0.5,"publicationDate":"2021-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45820801","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-04-16DOI: 10.14334/JITV.V26I1.2686
O. Sjofjan, Danung Nur Adli, M. Natsir, Y. F. Nuningtyas, Tika Septi Wardani, Ilmi Sholichatunnisa, Siti Nur Ulpah, Obbi Firmansyah
In this experiment, we investigate the effect of modified banana tuber meal (M-BTM) to substitute dietary maize in growing-finisher colored-feathered hybrid duck. One hundred and ninety six hybrid ducks (Pekin x Khaki Campbell) with 421.31 ± 0.183g body weight (BW) were allotted to 5 dietary treatments with 9 ducks (unsexed) per pen and 4 replications per treatment. These dietary treatments were: NC (negative control; maize-soyabean-meal based diet), BTM25 (25% maize was replaced by M-BTM-), BTM50 (50% maize was replaced by M-BTM), BTM75 (75% maize was replaced by M-BTM), and BTM100 (100% maize was replaced by M-BTM). The experimental design applied using completely randomize design (CRD). Data of this experiment were statistically analysed by one-way-analysis-of-variance of SAS University version 4.0 red hat (64-bit) University Online Edition. Result demonstrated that M-BTM improved significantly (p< 0.05) digestibility of dry matter and crude protein. It is concluded that M-BTM enhanced apparently growth performaces and digestibility parameters of colored-feathered hybrid duck (Pekin x Khaki Campbell).
本试验研究了改良香蕉块茎粉（M-BTM）替代日粮玉米在育成彩色羽毛鸭上的应用效果。将196只体重为421.31±0.183g的杂交鸭（Pekin x Khaki Campbell）分为5个日粮处理，每个处理9只（未固定），每个处理4次重复。这些日粮处理是：NC（阴性对照；玉米-豆粕日粮）、BTM25（25%的玉米被M-BTM-取代）、BTM50（50%的玉米被M-BTM取代）、BTM75（75%的玉米被M-BTM取代的）和BTM100（100%的玉米被M-BTM替代的）。实验设计采用完全随机设计（CRD）。通过SAS大学4.0版红帽子（64位）大学在线版的单向方差分析对本实验的数据进行统计分析。结果表明，M-BTM显著提高了干物质和粗蛋白的消化率（p<0.05）。结果表明，M-BTM显著提高了彩羽杂交鸭（Pekin x Khaki Campbell）的生长性能和消化率。
{"title":"Effect of Dietary Modified-Banana-Tuber Meal Substituting Dietary Corn on Growth Performance, Carcass Trait and Dietary-Nutrients Digestibility of Coloured-Feather Hybrid Duck","authors":"O. Sjofjan, Danung Nur Adli, M. Natsir, Y. F. Nuningtyas, Tika Septi Wardani, Ilmi Sholichatunnisa, Siti Nur Ulpah, Obbi Firmansyah","doi":"10.14334/JITV.V26I1.2686","DOIUrl":"https://doi.org/10.14334/JITV.V26I1.2686","url":null,"abstract":"In this experiment, we investigate the effect of modified banana tuber meal (M-BTM) to substitute dietary maize in growing-finisher colored-feathered hybrid duck. One hundred and ninety six hybrid ducks (Pekin x Khaki Campbell) with 421.31 ± 0.183g body weight (BW) were allotted to 5 dietary treatments with 9 ducks (unsexed) per pen and 4 replications per treatment. These dietary treatments were: NC (negative control; maize-soyabean-meal based diet), BTM25 (25% maize was replaced by M-BTM-), BTM50 (50% maize was replaced by M-BTM), BTM75 (75% maize was replaced by M-BTM), and BTM100 (100% maize was replaced by M-BTM). The experimental design applied using completely randomize design (CRD). Data of this experiment were statistically analysed by one-way-analysis-of-variance of SAS University version 4.0 red hat (64-bit) University Online Edition. Result demonstrated that M-BTM improved significantly (p< 0.05) digestibility of dry matter and crude protein. It is concluded that M-BTM enhanced apparently growth performaces and digestibility parameters of colored-feathered hybrid duck (Pekin x Khaki Campbell).","PeriodicalId":17806,"journal":{"name":"Jurnal Ilmu Ternak dan Veteriner","volume":" ","pages":""},"PeriodicalIF":0.5,"publicationDate":"2021-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45621868","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-03-16DOI: 10.14334/JITV.V26I1.2546
D. Endrawati, E. Kusumaningtyas
Trichophyton mentagrophytes and Microsporum canis are dermatophytes fungi which commonly infect animal and human. Conventional and molecular methods were used for identification of the fungus. The region of internal transcribed spacer (ITS) has a high probability for fungal identification. PCR-RFLP was reported as a useful method to differentiate dermatophytes fungi. The objective of the study was to compare molecular profile of T. mentagrophytes and M. canis based on the result of ITS fragment digestion using Dde I, Hinf I and Mva I. The molds were isolated from skin scrapping of 18 animals which showed dermatophytosis lesion. The isolated molds were grown on agar plate for 14 days of incubation at 37 o C and then identified based on macro and microscopic morphologies. Amplification of chitin synthase gene was used for confirmation and separation of dermatophytes from other fungi. ITS fragment was amplified and then digested using restriction enzymes Dde I, Hinf I and Mva I. The result showed that digestion products from ITS fragment of T. mentagrophytes and M. canis were different . The fragment 159 bp from Dde I, 374 bp from Hinf I and 89 bp from Mva I were present in T. mentagrophytes but absent in M. canis . Based on these results, specific RFLP profile of digestion ITS region by Dde I, Hinf I and Mva I can be used as a specific marker for species of dermatophytes fungi.
{"title":"Molecular Profile of Trichophyton mentagrophytes and Microsporum canis based on PCR-RFLP of Internal Transcribed Spacer","authors":"D. Endrawati, E. Kusumaningtyas","doi":"10.14334/JITV.V26I1.2546","DOIUrl":"https://doi.org/10.14334/JITV.V26I1.2546","url":null,"abstract":"Trichophyton mentagrophytes and Microsporum canis are dermatophytes fungi which commonly infect animal and human. Conventional and molecular methods were used for identification of the fungus. The region of internal transcribed spacer (ITS) has a high probability for fungal identification. PCR-RFLP was reported as a useful method to differentiate dermatophytes fungi. The objective of the study was to compare molecular profile of T. mentagrophytes and M. canis based on the result of ITS fragment digestion using Dde I, Hinf I and Mva I. The molds were isolated from skin scrapping of 18 animals which showed dermatophytosis lesion. The isolated molds were grown on agar plate for 14 days of incubation at 37 o C and then identified based on macro and microscopic morphologies. Amplification of chitin synthase gene was used for confirmation and separation of dermatophytes from other fungi. ITS fragment was amplified and then digested using restriction enzymes Dde I, Hinf I and Mva I. The result showed that digestion products from ITS fragment of T. mentagrophytes and M. canis were different . The fragment 159 bp from Dde I, 374 bp from Hinf I and 89 bp from Mva I were present in T. mentagrophytes but absent in M. canis . Based on these results, specific RFLP profile of digestion ITS region by Dde I, Hinf I and Mva I can be used as a specific marker for species of dermatophytes fungi.","PeriodicalId":17806,"journal":{"name":"Jurnal Ilmu Ternak dan Veteriner","volume":" ","pages":""},"PeriodicalIF":0.5,"publicationDate":"2021-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49134375","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-03-16DOI: 10.14334/JITV.V26I1.2508
A. Dargantes, A. H. Wardhana, J. Abella, Milkesidick R Sequito, S. Reid, D. B. Copeman
The pathogenicity of 10 isolates of T. evansi collected from Mindanao, Philippines, and one isolate from East Java, Indonesia was determined and compared. The susceptibility of these isolates against diminazene aceturate, melarsomine dihydrochloride, suramin and quinapyramine sulphate/chloride were also tested. Twenty-five mice were infected intraperitoneally with each isolate and 20 were treated with the 4 drugs (5 mice/drug) while 5 infected mice and 7 uninfected mice served as infected-untreated and uninfected controls, respectively. Treatment was carried out 24 hours post-infection and parasitemia was monitored for 35 days. Mice infected with Philippine isolates significantly died earlier (5-11 days) than those infected with the Indonesian isolate (14-16 days). The prepatent period for Philippine isolates (3-8 days) was significantly shorter than the Indonesian strain (11-13 days). Trypanosomes were not observed in the blood of mice infected with any of the Philippine isolates when treated with quinapyramine sulphate/chloride, melarsomine dihydrochloride or suramin. Two of 10 mice infected with either C4 or A9 Philippine isolates and treated with diminazene aceturate had parasitemia on days 29 and 31, respectively. It is concluded that isolates of T. evansi from Mindanao, Philippines, are more pathogenic than the isolate from East Java, Indonesia. This study also indicated that quinapyramine sulphate/chloride, melarsomine dihydrochloride and suramin are effective against these T. evansi isolates from Mindanao, Philippines and East Java, Indonesia, while two of the Mindanao isolates are resistant to diminazene. This information is valuable in the enhancement of the control strategy against surra in the Philippines and Indonesia.
{"title":"Pathogenicity of Philippine and Indonesian Trypanosoma evansi Isolates in Mice and Their Responses to Trypanocides","authors":"A. Dargantes, A. H. Wardhana, J. Abella, Milkesidick R Sequito, S. Reid, D. B. Copeman","doi":"10.14334/JITV.V26I1.2508","DOIUrl":"https://doi.org/10.14334/JITV.V26I1.2508","url":null,"abstract":"The pathogenicity of 10 isolates of T. evansi collected from Mindanao, Philippines, and one isolate from East Java, Indonesia was determined and compared. The susceptibility of these isolates against diminazene aceturate, melarsomine dihydrochloride, suramin and quinapyramine sulphate/chloride were also tested. Twenty-five mice were infected intraperitoneally with each isolate and 20 were treated with the 4 drugs (5 mice/drug) while 5 infected mice and 7 uninfected mice served as infected-untreated and uninfected controls, respectively. Treatment was carried out 24 hours post-infection and parasitemia was monitored for 35 days. Mice infected with Philippine isolates significantly died earlier (5-11 days) than those infected with the Indonesian isolate (14-16 days). The prepatent period for Philippine isolates (3-8 days) was significantly shorter than the Indonesian strain (11-13 days). Trypanosomes were not observed in the blood of mice infected with any of the Philippine isolates when treated with quinapyramine sulphate/chloride, melarsomine dihydrochloride or suramin. Two of 10 mice infected with either C4 or A9 Philippine isolates and treated with diminazene aceturate had parasitemia on days 29 and 31, respectively. It is concluded that isolates of T. evansi from Mindanao, Philippines, are more pathogenic than the isolate from East Java, Indonesia. This study also indicated that quinapyramine sulphate/chloride, melarsomine dihydrochloride and suramin are effective against these T. evansi isolates from Mindanao, Philippines and East Java, Indonesia, while two of the Mindanao isolates are resistant to diminazene. This information is valuable in the enhancement of the control strategy against surra in the Philippines and Indonesia.","PeriodicalId":17806,"journal":{"name":"Jurnal Ilmu Ternak dan Veteriner","volume":" ","pages":""},"PeriodicalIF":0.5,"publicationDate":"2021-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47318116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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