Pub Date : 2021-03-16DOI: 10.14334/JITV.V26I1.2670
D. C. Widianingrum, S. I. Salasia
The virgin coconut oil (VCO) contains bioactive that induce immunity against infectious diseases. This study aim to determine the immunomodulatory effects of VCO based on the activity of superoxide dismutase (SOD), lymphocyte proliferation, and histopathological examination in liver and kidney of rats infected with Staphylococcus aureus . The VCO was given intragastrically to rats with a dose of 250 µL for one week. The rats were infected with S. aureus at 5x10 2 bacterial cells intraperitoneally. Twenty (20) female Wistar rats of one month old were divided into four groups. The negative control group (C-): without treatment, AV group: infected with S. aureus followed by VCO treatment; VA group: pretreated with VCO followed by S. aureus infection, and positive control group (C+): were infected with S. aureus without VCO. All rats were euthanized and necropsied based on the animal ethic standard. Plasma samples were taken to evaluate SOD activity, and lymphocytes were isolated from the spleen to determine their proliferative ability. Livers and kidneys were collected for a histopathology examination. The results showed that the VA group had the highest SOD activity on the 4 th week (41.50 ± 3.56 %) and lymphocyte proliferation (0.3018) compared to all treatments, indicating immunomodulatory effects of VCO. Liver of treatments group showed leucocytes infiltration, no hemorrhages (VA); the hepatocytes with normal cells (VA). Kidney of treatments group showed leucocytes infiltration (AV); normal epithelial glomerulus and tubulus cells, still found hemorrhage (VA). These studies indicated that VCO has a potential role as an immunomodulator, hepatoprotectant, and nephroprotectant.
{"title":"Immunomodulatory Effect of Virgin Coconut Oil in Wistar Rats Infected with Staphylococcus aureus","authors":"D. C. Widianingrum, S. I. Salasia","doi":"10.14334/JITV.V26I1.2670","DOIUrl":"https://doi.org/10.14334/JITV.V26I1.2670","url":null,"abstract":"The virgin coconut oil (VCO) contains bioactive that induce immunity against infectious diseases. This study aim to determine the immunomodulatory effects of VCO based on the activity of superoxide dismutase (SOD), lymphocyte proliferation, and histopathological examination in liver and kidney of rats infected with Staphylococcus aureus . The VCO was given intragastrically to rats with a dose of 250 µL for one week. The rats were infected with S. aureus at 5x10 2 bacterial cells intraperitoneally. Twenty (20) female Wistar rats of one month old were divided into four groups. The negative control group (C-): without treatment, AV group: infected with S. aureus followed by VCO treatment; VA group: pretreated with VCO followed by S. aureus infection, and positive control group (C+): were infected with S. aureus without VCO. All rats were euthanized and necropsied based on the animal ethic standard. Plasma samples were taken to evaluate SOD activity, and lymphocytes were isolated from the spleen to determine their proliferative ability. Livers and kidneys were collected for a histopathology examination. The results showed that the VA group had the highest SOD activity on the 4 th week (41.50 ± 3.56 %) and lymphocyte proliferation (0.3018) compared to all treatments, indicating immunomodulatory effects of VCO. Liver of treatments group showed leucocytes infiltration, no hemorrhages (VA); the hepatocytes with normal cells (VA). Kidney of treatments group showed leucocytes infiltration (AV); normal epithelial glomerulus and tubulus cells, still found hemorrhage (VA). These studies indicated that VCO has a potential role as an immunomodulator, hepatoprotectant, and nephroprotectant.","PeriodicalId":17806,"journal":{"name":"Jurnal Ilmu Ternak dan Veteriner","volume":" ","pages":""},"PeriodicalIF":0.5,"publicationDate":"2021-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46733439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-03-16DOI: 10.14334/JITV.V26I1.2584
Syifa Khairunnisa, S. Novelina, N. Hilmia, D. N. Hadi, D. Rahmat, M. F. Ulum
This research aimed to estimate carcass quality of Pasundan cattle using ultrasound imaging based on Body Condition Score (BCS). Total 31 head of female cattle with age ranging from 4 to 7 years from Beef Cattle Breading and Artificial Insemination Center at Ciamis West Java of Indonesia with BCS ranging from 1.0-4.0. The marbling score, intramuscular fat (IMF), backfat thickness (LP), and thickness musculus of m. longissimus dorsi (LD), m. psoas major (PM), m. psoas minor (PMN), m. gluteus medius (GM) and m. biceps femoris (BF) were scanned using ultrasound on 3 different locations, i.e. on 12 th -13 th ribs (thorax), lumbar 4 th - 5 th (lumbar), and between tuber coxae and tuber ischii (gluteal) with 5 MHz frequency of convex transducer. The results showed that BCS increased when LP, marbling score and IMF from m. LD, m. PM, m. PMN, and m. GM was rising. Pasundan cattle showed marbling scores ranging from score 1 to 5 and percentage IMF ranging from 2.62% to 4.82%. Body Condition Score affected carcass quality of Pasundan cattle on parameters such as musculus thickness, marbling score, and intramuscular fat (IMF) from ultrasound imaging of m. LD, m. PM. m. PMN, m. GM, and m. BF.
{"title":"Ultrasound Imaging to Estimate Carcass Quality of Pasundan Cattle based on Body Condition Score","authors":"Syifa Khairunnisa, S. Novelina, N. Hilmia, D. N. Hadi, D. Rahmat, M. F. Ulum","doi":"10.14334/JITV.V26I1.2584","DOIUrl":"https://doi.org/10.14334/JITV.V26I1.2584","url":null,"abstract":"This research aimed to estimate carcass quality of Pasundan cattle using ultrasound imaging based on Body Condition Score (BCS). Total 31 head of female cattle with age ranging from 4 to 7 years from Beef Cattle Breading and Artificial Insemination Center at Ciamis West Java of Indonesia with BCS ranging from 1.0-4.0. The marbling score, intramuscular fat (IMF), backfat thickness (LP), and thickness musculus of m. longissimus dorsi (LD), m. psoas major (PM), m. psoas minor (PMN), m. gluteus medius (GM) and m. biceps femoris (BF) were scanned using ultrasound on 3 different locations, i.e. on 12 th -13 th ribs (thorax), lumbar 4 th - 5 th (lumbar), and between tuber coxae and tuber ischii (gluteal) with 5 MHz frequency of convex transducer. The results showed that BCS increased when LP, marbling score and IMF from m. LD, m. PM, m. PMN, and m. GM was rising. Pasundan cattle showed marbling scores ranging from score 1 to 5 and percentage IMF ranging from 2.62% to 4.82%. Body Condition Score affected carcass quality of Pasundan cattle on parameters such as musculus thickness, marbling score, and intramuscular fat (IMF) from ultrasound imaging of m. LD, m. PM. m. PMN, m. GM, and m. BF.","PeriodicalId":17806,"journal":{"name":"Jurnal Ilmu Ternak dan Veteriner","volume":"26 1","pages":""},"PeriodicalIF":0.5,"publicationDate":"2021-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45565363","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-12-30DOI: 10.14334/jitv.v26i1.2675
Sunda porcupine (Hystrix javanica) is one of the Indonesian endemic species which is often sought after for their meat. Although it is becoming increasingly popular for extreme culinary, information regarding biological risks arising from this wildlife is very limited. This study aimed to assess potential zoonotic faecal bacteria carried by Sunda porcupine with culture-dependant approach and to investigate whether antimicrobial resistant isolates can be found in wildlife. A total 22 faecal samples were collected from captive Sunda porcupine and tested for the presence of pathogens in selective media for Salmonella and Listeria. After inoculating the samples in Rappaport-Vassiliadis (RV) Salmonella enrichment broth, two samples (9%) were regarded as positive for Salmonella in this presumptive test which indicated by growth black colonies on xylose lysine deoxycholate (XLD) agar. Meanwhile, the presence for Listeria was presumptively positive in all samples (100%), indicated by black colour appearance in Listeria isolation transwab. In total, 38 bacterial isolates were successfully purified, preserved and subjected for antimicrobial susceptibility testing (AST) by disk diffusion method. Resistance to ceftriaxone (3rd generation cephalosporins) was not detected while resistance to one or two antimicrobials was observed in seven isolates. Further, 16S rRNA bacterial identification was performed for selected isolates and based on sequence similarity on GenBank® databases and phylogenetic tree construction, those isolates were denoted as Pseudomonas xinjiangensis (XG4.4), Shigella sonnei (XD8.2 and G11.3), Proteus mirabilis (XH3.3, H4.2, and E1.2) and Klebsiella quasipneumoniae subsp. similipneumoniae (XF4.2). All identified isolates were sensitive to amikacin, amoxicillin-clavulanic acid, cefoxitin and ceftriaxone, except for one isolate Shigella sonnei (XD8.2) which was resistant to cefoxitin. Further research to confirm the pathogenicity of the isolates is still needed but based on these results, we support the hypothesies that Sunda porcupine is potential as a reservoir pathogenic bacteria and preventive measures are crucial to prevent transmission when processing this bushmeat.
{"title":"Potential Zoonotic Faecal Bacteria and Antimicrobial Resistance from Sunda Porcupine (Hystrix javanica): A Preliminary Study","authors":"","doi":"10.14334/jitv.v26i1.2675","DOIUrl":"https://doi.org/10.14334/jitv.v26i1.2675","url":null,"abstract":"Sunda porcupine (Hystrix javanica) is one of the Indonesian endemic species which is often sought after for their meat. Although it is becoming increasingly popular for extreme culinary, information regarding biological risks arising from this wildlife is very limited. This study aimed to assess potential zoonotic faecal bacteria carried by Sunda porcupine with culture-dependant approach and to investigate whether antimicrobial resistant isolates can be found in wildlife. A total 22 faecal samples were collected from captive Sunda porcupine and tested for the presence of pathogens in selective media for Salmonella and Listeria. After inoculating the samples in Rappaport-Vassiliadis (RV) Salmonella enrichment broth, two samples (9%) were regarded as positive for Salmonella in this presumptive test which indicated by growth black colonies on xylose lysine deoxycholate (XLD) agar. Meanwhile, the presence for Listeria was presumptively positive in all samples (100%), indicated by black colour appearance in Listeria isolation transwab. In total, 38 bacterial isolates were successfully purified, preserved and subjected for antimicrobial susceptibility testing (AST) by disk diffusion method. Resistance to ceftriaxone (3rd generation cephalosporins) was not detected while resistance to one or two antimicrobials was observed in seven isolates. Further, 16S rRNA bacterial identification was performed for selected isolates and based on sequence similarity on GenBank® databases and phylogenetic tree construction, those isolates were denoted as Pseudomonas xinjiangensis (XG4.4), Shigella sonnei (XD8.2 and G11.3), Proteus mirabilis (XH3.3, H4.2, and E1.2) and Klebsiella quasipneumoniae subsp. similipneumoniae (XF4.2). All identified isolates were sensitive to amikacin, amoxicillin-clavulanic acid, cefoxitin and ceftriaxone, except for one isolate Shigella sonnei (XD8.2) which was resistant to cefoxitin. Further research to confirm the pathogenicity of the isolates is still needed but based on these results, we support the hypothesies that Sunda porcupine is potential as a reservoir pathogenic bacteria and preventive measures are crucial to prevent transmission when processing this bushmeat.","PeriodicalId":17806,"journal":{"name":"Jurnal Ilmu Ternak dan Veteriner","volume":" ","pages":""},"PeriodicalIF":0.5,"publicationDate":"2020-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46282857","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-12-14DOI: 10.14334/jitv.v25i4.2499
C. Utama, Z. Zuprizal, C. Hanim, Wihandoyo Wihandoyo
This research was aimed to assess the effectiveness of processed wheat pollard -based rations to increase the productivity of Kampung chickens raised until 8 weeks old. The research was carried out in a completely randomized design with 5 treatments and 4 replications. The treatments consisted of wheat pollard based ration (WP), wheat pollard based ration plus probiotic mixed culture (WPPro), wheat Pollard as prebiotic mixed (WPPre) , wheat pollard as synbiotic mixed culture ration 40% (WPS40), wheat pollard as synbiotic mixed culture ration 60% (WPS60) . The parameters observed were feed consumption, final body weight, feed conversion ratio (FCR), weight gain, nitrogen retention, income over feed and chick cost (IOFCC) and profiles of intestinal villus. Results showed a significant effect of the treatments on the final body weight, weight gain, nitrogen retention, IOFCC and profiles of small intestinal villus (duodenum, jejenum, ileum). It was concluded that the inclusion of 40% wheat pollard synbiotic mixed culture (WPS 40) in the ration was able to increase the productivity of kampung chickens reared until 8 weeks old.
{"title":"Effects of Probiotic, Prebiotic, and Synbiotic Mixed Culture Based on Wheat Pollard on Productivity of Kampung’s Chicken","authors":"C. Utama, Z. Zuprizal, C. Hanim, Wihandoyo Wihandoyo","doi":"10.14334/jitv.v25i4.2499","DOIUrl":"https://doi.org/10.14334/jitv.v25i4.2499","url":null,"abstract":"This research was aimed to assess the effectiveness of processed wheat pollard -based rations to increase the productivity of Kampung chickens raised until 8 weeks old. The research was carried out in a completely randomized design with 5 treatments and 4 replications. The treatments consisted of wheat pollard based ration (WP), wheat pollard based ration plus probiotic mixed culture (WPPro), wheat Pollard as prebiotic mixed (WPPre) , wheat pollard as synbiotic mixed culture ration 40% (WPS40), wheat pollard as synbiotic mixed culture ration 60% (WPS60) . The parameters observed were feed consumption, final body weight, feed conversion ratio (FCR), weight gain, nitrogen retention, income over feed and chick cost (IOFCC) and profiles of intestinal villus. Results showed a significant effect of the treatments on the final body weight, weight gain, nitrogen retention, IOFCC and profiles of small intestinal villus (duodenum, jejenum, ileum). It was concluded that the inclusion of 40% wheat pollard synbiotic mixed culture (WPS 40) in the ration was able to increase the productivity of kampung chickens reared until 8 weeks old.","PeriodicalId":17806,"journal":{"name":"Jurnal Ilmu Ternak dan Veteriner","volume":"1 1","pages":""},"PeriodicalIF":0.5,"publicationDate":"2020-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42783103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-12-14DOI: 10.14334/jitv.v25i4.2555
Masume Ghorbani Vahed, R. Khanbabaee, M. Shariati, M. Edalatmanesh
This research was conducted in order to determine the effects of omega-3 on oocyte in vitro maturation and the level of expression of tribbles (TRIB1, TRIB2 and TRIB3 genes) in cumulus cells. Eight-ten weeks old NMRI mice were super-ovulated using 7.5 IU pregnant mare’s serum gonadotropin (PMSG, Intraperitoneal) and they were killed after 44 hours and their ovaries were removed. The oocytes were used for in vitro maturation and the cumulus-oocyte complexes (COCs) were released. Cumulus cells and oocytes were assigned into control, ethanol-treated and groups exposed to 10 and 100 μg/ml of omega-3. The cells were prepared to assess the maturation stage in order to evaluate the gene expression level. The data were statistically analyzed. Exposing oocytes to low dose (10 μg/ml) and high dose (100 μg/ml) of omega-3 resulted in a reduced rate of GV-stage oocytes, decreased MI-oocytes and increased MII-oocytes. The enhanced maturity of COCs was also detected in response to a high dose of omega-3 (100 μg/ml). Exposure of cumulus cells to omega-3 (10 and 100 μg/ml) induced TRIB2 and inhibited TRIB3 gene expression level; however, TRIB1 gene expression level increased and decreased in response to low (10 μg/ml) and high (100 μg/ml) concentrations of omega-3, respectively. The addition of omega-3 to the environment of oocytes or cumulus cells affected the maturation of oocytes and cumulus cells, which was followed by the differential expression of TRIB genes, suggesting that there was a role of fatty acid metabolism in the differentiation and maturation of cumulus cells.
{"title":"Improving the Effects of Omega-3 Fatty Acid on the In Vitro Maturation of Oocytes","authors":"Masume Ghorbani Vahed, R. Khanbabaee, M. Shariati, M. Edalatmanesh","doi":"10.14334/jitv.v25i4.2555","DOIUrl":"https://doi.org/10.14334/jitv.v25i4.2555","url":null,"abstract":"This research was conducted in order to determine the effects of omega-3 on oocyte in vitro maturation and the level of expression of tribbles (TRIB1, TRIB2 and TRIB3 genes) in cumulus cells. Eight-ten weeks old NMRI mice were super-ovulated using 7.5 IU pregnant mare’s serum gonadotropin (PMSG, Intraperitoneal) and they were killed after 44 hours and their ovaries were removed. The oocytes were used for in vitro maturation and the cumulus-oocyte complexes (COCs) were released. Cumulus cells and oocytes were assigned into control, ethanol-treated and groups exposed to 10 and 100 μg/ml of omega-3. The cells were prepared to assess the maturation stage in order to evaluate the gene expression level. The data were statistically analyzed. Exposing oocytes to low dose (10 μg/ml) and high dose (100 μg/ml) of omega-3 resulted in a reduced rate of GV-stage oocytes, decreased MI-oocytes and increased MII-oocytes. The enhanced maturity of COCs was also detected in response to a high dose of omega-3 (100 μg/ml). Exposure of cumulus cells to omega-3 (10 and 100 μg/ml) induced TRIB2 and inhibited TRIB3 gene expression level; however, TRIB1 gene expression level increased and decreased in response to low (10 μg/ml) and high (100 μg/ml) concentrations of omega-3, respectively. The addition of omega-3 to the environment of oocytes or cumulus cells affected the maturation of oocytes and cumulus cells, which was followed by the differential expression of TRIB genes, suggesting that there was a role of fatty acid metabolism in the differentiation and maturation of cumulus cells.","PeriodicalId":17806,"journal":{"name":"Jurnal Ilmu Ternak dan Veteriner","volume":"25 1","pages":"153-161"},"PeriodicalIF":0.5,"publicationDate":"2020-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42748570","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-12-14DOI: 10.14334/jitv.v25i4.2526
Gunawan Sitanggang, R. I. Arifiantini, J. Jakaria
The objective of this study was to evaluate effect of genetic and non-genetic factors on semen characteristics including ejaculate volume, sperm concentration, total sperm number and sperm moetility of Bali cattle. Semen data were collected from the National Centre of Artificial Insemination at Singosari, Malang, East Java, Indonesia. A total of 3,847 ejaculates of 17 Bali bulls from 2014 to 2016 were collected and evaluated. Data were analyzed by restricted maximum likelihood (REML) method using mixed models which the bull was a random effect, while age of bull, season of collection, frequency of ejaculation and collection intervals were the fixed effects. Results showed that age significantly affected all semen characteristics (P<0.01). Season affected only on sperm motility (P<0.01). Effect of frequency of ejaculation and collection intervals was significant on all studied variables (P<0.01), except sperm motility. Repeatability of ejaculate volume, sperm concentration, total sperm number and sperm motility was 0.43; 0.35; 0.32 and 0.31, respectively. It is concluded that age, frequency of ejaculation and collection intervals were the most factors affected semen characteristics of Bali cattle. Repeatability estimations of semen characteristics were moderate to high.
{"title":"Genetic and Non-Genetic Effects on Semen Characteristics of Bali Cattle (Bos javanicus)","authors":"Gunawan Sitanggang, R. I. Arifiantini, J. Jakaria","doi":"10.14334/jitv.v25i4.2526","DOIUrl":"https://doi.org/10.14334/jitv.v25i4.2526","url":null,"abstract":"The objective of this study was to evaluate effect of genetic and non-genetic factors on semen characteristics including ejaculate volume, sperm concentration, total sperm number and sperm moetility of Bali cattle. Semen data were collected from the National Centre of Artificial Insemination at Singosari, Malang, East Java, Indonesia. A total of 3,847 ejaculates of 17 Bali bulls from 2014 to 2016 were collected and evaluated. Data were analyzed by restricted maximum likelihood (REML) method using mixed models which the bull was a random effect, while age of bull, season of collection, frequency of ejaculation and collection intervals were the fixed effects. Results showed that age significantly affected all semen characteristics (P<0.01). Season affected only on sperm motility (P<0.01). Effect of frequency of ejaculation and collection intervals was significant on all studied variables (P<0.01), except sperm motility. Repeatability of ejaculate volume, sperm concentration, total sperm number and sperm motility was 0.43; 0.35; 0.32 and 0.31, respectively. It is concluded that age, frequency of ejaculation and collection intervals were the most factors affected semen characteristics of Bali cattle. Repeatability estimations of semen characteristics were moderate to high.","PeriodicalId":17806,"journal":{"name":"Jurnal Ilmu Ternak dan Veteriner","volume":" ","pages":""},"PeriodicalIF":0.5,"publicationDate":"2020-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47191682","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-12-14DOI: 10.14334/jitv.v25i4.2525
Lailatul Rohmah, S. Darwati, N. Ulupi, I. Khaerunnisa, C. Sumantri
The prolactin gene (PRL) is a gene that controls the incubation and egg production in laying chickens. The nature of incubation will reduce egg production and disrupt the reproductive system in local chickens. The purpose of this study was to identify the polymorphism of prolactin genes in IPB-D1 chickens using the direct sequencing method. The polymorphism of the exon 5 prolactin gene was carried out on 46 samples of IPB-D1 chicken DNA which was a collection of the Division of Animal Genetics and Breeding, Faculty of Animal Science IPB. DNA sequences as a reference for designing exon 5 primers were obtained from the National Center for Biotechnology Information (NCBI) with the GenBank access code: AF288765.2. DNA extraction was carried out using the phenol-chloroform technique. DNA amplification resulted in a PCR product with a size of 557 bp. In this study, the genotype frequency, allele frequency, heterozygosity value and Hardy-Weinberg equilibrium were calculated. The results of the study found 5 SNPs in exon 5, namely g.7823A>G, g.7835A>G, g.7886T>A, g.8052T>C, and g.8069T>C. All SNPs are polymorphic and in Hardy-Weinberg equilibrium except g.8052T>C. The g.7823A>G, g.7835A>G, g.8052T>C SNPs are synonymous mutations that do not change amino acids, while the g.7886T>A and g.8069T>C SNPs are non-synonymous that change amino acids. Both g.7886T>A and g.8069T>C SNPs are potential as a marker assisted selection for the characteristics of egg production in IPB-D1 chickens.
{"title":"Novel Mutation of Exon 5 Prolactin Gene in IPB-D1 Chicken","authors":"Lailatul Rohmah, S. Darwati, N. Ulupi, I. Khaerunnisa, C. Sumantri","doi":"10.14334/jitv.v25i4.2525","DOIUrl":"https://doi.org/10.14334/jitv.v25i4.2525","url":null,"abstract":"The prolactin gene (PRL) is a gene that controls the incubation and egg production in laying chickens. The nature of incubation will reduce egg production and disrupt the reproductive system in local chickens. The purpose of this study was to identify the polymorphism of prolactin genes in IPB-D1 chickens using the direct sequencing method. The polymorphism of the exon 5 prolactin gene was carried out on 46 samples of IPB-D1 chicken DNA which was a collection of the Division of Animal Genetics and Breeding, Faculty of Animal Science IPB. DNA sequences as a reference for designing exon 5 primers were obtained from the National Center for Biotechnology Information (NCBI) with the GenBank access code: AF288765.2. DNA extraction was carried out using the phenol-chloroform technique. DNA amplification resulted in a PCR product with a size of 557 bp. In this study, the genotype frequency, allele frequency, heterozygosity value and Hardy-Weinberg equilibrium were calculated. The results of the study found 5 SNPs in exon 5, namely g.7823A>G, g.7835A>G, g.7886T>A, g.8052T>C, and g.8069T>C. All SNPs are polymorphic and in Hardy-Weinberg equilibrium except g.8052T>C. The g.7823A>G, g.7835A>G, g.8052T>C SNPs are synonymous mutations that do not change amino acids, while the g.7886T>A and g.8069T>C SNPs are non-synonymous that change amino acids. Both g.7886T>A and g.8069T>C SNPs are potential as a marker assisted selection for the characteristics of egg production in IPB-D1 chickens.","PeriodicalId":17806,"journal":{"name":"Jurnal Ilmu Ternak dan Veteriner","volume":" ","pages":""},"PeriodicalIF":0.5,"publicationDate":"2020-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48271882","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-12-14DOI: 10.14334/jitv.v25i4.2541
Y. Yendraliza, M. Rodiallah, T. Astuti, Elfawati Elfawati
The purpose of this study was to determine reproductive efficiency, population dynamics, natural increase and estimated output of the Kuantan cattle in the Kuansing Regency, Province of Riau. A total of 311 Kuantan cattle and 99 Kuantan cattle farmers were used in this study through a survey study. Respondent samples were taken from seven districts. Data sampling using purposive sampling with survey methods. Data collection was carried out by interviewing farmers and observing and was analyzed descriptively. Parameters measured were reproductive efficiency, natural increase, estimated output and population dynamics of Kuantan cattle. Results showed that the reproductive efficiency of Kuantan cattle was 1.04%, natural increase 5.14%, the balance of male and female 1: 5, the value of male NRR 50% and female NRR 100.56%, total cattle out 18.69% and total incoming cattle 18.69%, output value 48.88% and estimated population dynamics 2.85%. In conclusion, Kuantan cattle reproduction has not been efficient with the natural increase of the Kuantan cattle was very low, and the replacement stock availability for male and female cattle has not been fulfilled. It is recommended not to release Kuantan cattle in the next 5 years to maintain population balance.
{"title":"Reproduction Status and Population Dynamic of Kuantan Cattle in the Kuantan Singingi Regency","authors":"Y. Yendraliza, M. Rodiallah, T. Astuti, Elfawati Elfawati","doi":"10.14334/jitv.v25i4.2541","DOIUrl":"https://doi.org/10.14334/jitv.v25i4.2541","url":null,"abstract":"The purpose of this study was to determine reproductive efficiency, population dynamics, natural increase and estimated output of the Kuantan cattle in the Kuansing Regency, Province of Riau. A total of 311 Kuantan cattle and 99 Kuantan cattle farmers were used in this study through a survey study. Respondent samples were taken from seven districts. Data sampling using purposive sampling with survey methods. Data collection was carried out by interviewing farmers and observing and was analyzed descriptively. Parameters measured were reproductive efficiency, natural increase, estimated output and population dynamics of Kuantan cattle. Results showed that the reproductive efficiency of Kuantan cattle was 1.04%, natural increase 5.14%, the balance of male and female 1: 5, the value of male NRR 50% and female NRR 100.56%, total cattle out 18.69% and total incoming cattle 18.69%, output value 48.88% and estimated population dynamics 2.85%. In conclusion, Kuantan cattle reproduction has not been efficient with the natural increase of the Kuantan cattle was very low, and the replacement stock availability for male and female cattle has not been fulfilled. It is recommended not to release Kuantan cattle in the next 5 years to maintain population balance.","PeriodicalId":17806,"journal":{"name":"Jurnal Ilmu Ternak dan Veteriner","volume":" ","pages":""},"PeriodicalIF":0.5,"publicationDate":"2020-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44115602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-12-14DOI: 10.14334/jitv.v25i4.2514
Agus Hadi Prayitno, T. Rahman
This study aimed to determine the effect of edamame flour filler substitution on the chemical quality of culled duck meatballs. The research material consisted of culled duck meat, tapioca flour, edamame flour, albumen, garlic, onion, salt, pepper, monosodium glutamate, sodium tripolyphosphate, and ice. The edamame flour filler substitution treatments were P0 (0%), P1 (5%), P2 (10%), P3 (15%), and P4 (20%) from total filler. Each treatment consisted of five replications. The parameters which tested were moisture, protein, fat, fiber, and ash contents. Data on chemical quality results were analyzed by analysis of variance using completely randomized design and if there was significantly different (P<0.01), then it’ll be further tested by the Duncan's New Multiple Range Test. Results showed that the substitution of edamame flour filler starting from the level of 5% was significantly affected water content, protein, fat, fiber, and ash of culled duck meatballs. Edamame flour can be used as a filler substitution up to 20% level by giving a good effect on increasing the meatball protein content, but also having a negative impact with increasing the fat content of culled duck meatballs.
本研究旨在确定毛豆粉填料替代对扑杀鸭肉丸子化学质量的影响。研究材料包括扑杀的鸭肉、木薯粉、毛豆粉、蛋白、大蒜、洋葱、盐、胡椒、味精、三聚磷酸钠和冰。毛豆粉填料替代处理的总填料为P0（0%）、P1（5%）、P2（10%）、P3（15%）和P4（20%）。每种处理包括五次重复。测试的参数包括水分、蛋白质、脂肪、纤维和灰分。化学质量结果的数据采用完全随机设计，通过方差分析进行分析，如果有显著差异（P<0.01），则将通过Duncan’s New Multiple Range Test进行进一步检验。结果表明，从5%的水平开始，毛豆粉填料的替代对鸭肉丸子的含水量、蛋白质、脂肪、纤维和灰分均有显著影响。Edamame面粉可以用作高达20%水平的填料替代品，因为它对提高肉丸蛋白质含量有很好的效果，但对增加扑杀鸭肉丸的脂肪含量也有负面影响。
{"title":"Chemical Quality of Culled Duck Meatball (Anasplathyryncos) Substituted with Edamame Flour (Glycine max (L) Merril) Filler","authors":"Agus Hadi Prayitno, T. Rahman","doi":"10.14334/jitv.v25i4.2514","DOIUrl":"https://doi.org/10.14334/jitv.v25i4.2514","url":null,"abstract":"This study aimed to determine the effect of edamame flour filler substitution on the chemical quality of culled duck meatballs. The research material consisted of culled duck meat, tapioca flour, edamame flour, albumen, garlic, onion, salt, pepper, monosodium glutamate, sodium tripolyphosphate, and ice. The edamame flour filler substitution treatments were P0 (0%), P1 (5%), P2 (10%), P3 (15%), and P4 (20%) from total filler. Each treatment consisted of five replications. The parameters which tested were moisture, protein, fat, fiber, and ash contents. Data on chemical quality results were analyzed by analysis of variance using completely randomized design and if there was significantly different (P<0.01), then it’ll be further tested by the Duncan's New Multiple Range Test. Results showed that the substitution of edamame flour filler starting from the level of 5% was significantly affected water content, protein, fat, fiber, and ash of culled duck meatballs. Edamame flour can be used as a filler substitution up to 20% level by giving a good effect on increasing the meatball protein content, but also having a negative impact with increasing the fat content of culled duck meatballs.","PeriodicalId":17806,"journal":{"name":"Jurnal Ilmu Ternak dan Veteriner","volume":" ","pages":""},"PeriodicalIF":0.5,"publicationDate":"2020-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45498460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-12-03DOI: 10.14334/jitv.v25i4.2515
I. Mareta, G. Nathaniel, T. Yudiarti, E. Widiastuti, H. I. Wahyuni, S. Sugiharto
This study was aimed to evaluate effect of a mixture of Averrhoa bilimbi fruit filtrate and shrimp paste ( Mysis sp.) on the growth performance, blood profile, selected intestinal bacterial number and pH value of broiler digestive tract. The mixture of A. bilimbi fruit filtrate and shrimp paste were incubated for 4 days and were then used in the experiment. For in vivo experiment, 40 day-old Lohmann broiler chicks were distributed randomly to two treatment groups, i.e., control (T1) and chickens given the mixture of 10% A. bilimbi fruit filtrate and shrimp paste in drinking water (T2). Body weight and feed intake were recorded weekly. At day 42, 2 birds from each pen (8 chicks per treatment group) were taken for blood and digesta collection. Internal organ weight and carcass traits were determined thereafter. Feed conversion ratio (FCR) was lower (P<0.05) on treatment group than control. Hemoglobin and mean corpuscular volume (MCV) of treatment group were higher (P<0.05) than that of control. Total cholesterol was higher (P<0.05) in treatment group than that in control. Total cecum coliform was lower (P<0.05) in treatment group than that in control. The pH values of the small intestinal segments (jejunum, ileum, cecum) was lower (P<0.05) in treatment group than that in control group. In conclusion, administration of the blends of A. bilimbi fruit filtrate and shrimp paste into drinking water improved FCR, increased hemoglobin and MCV values, decreased gut pH and cecal coliform of broiler chickens.
{"title":"Effect of Averrhoa bilimbi Fruit Filtrate and Shrimp Paste Mixture on Performance, Gut Microbes and Blood Profile of Broilers","authors":"I. Mareta, G. Nathaniel, T. Yudiarti, E. Widiastuti, H. I. Wahyuni, S. Sugiharto","doi":"10.14334/jitv.v25i4.2515","DOIUrl":"https://doi.org/10.14334/jitv.v25i4.2515","url":null,"abstract":"This study was aimed to evaluate effect of a mixture of Averrhoa bilimbi fruit filtrate and shrimp paste ( Mysis sp.) on the growth performance, blood profile, selected intestinal bacterial number and pH value of broiler digestive tract. The mixture of A. bilimbi fruit filtrate and shrimp paste were incubated for 4 days and were then used in the experiment. For in vivo experiment, 40 day-old Lohmann broiler chicks were distributed randomly to two treatment groups, i.e., control (T1) and chickens given the mixture of 10% A. bilimbi fruit filtrate and shrimp paste in drinking water (T2). Body weight and feed intake were recorded weekly. At day 42, 2 birds from each pen (8 chicks per treatment group) were taken for blood and digesta collection. Internal organ weight and carcass traits were determined thereafter. Feed conversion ratio (FCR) was lower (P<0.05) on treatment group than control. Hemoglobin and mean corpuscular volume (MCV) of treatment group were higher (P<0.05) than that of control. Total cholesterol was higher (P<0.05) in treatment group than that in control. Total cecum coliform was lower (P<0.05) in treatment group than that in control. The pH values of the small intestinal segments (jejunum, ileum, cecum) was lower (P<0.05) in treatment group than that in control group. In conclusion, administration of the blends of A. bilimbi fruit filtrate and shrimp paste into drinking water improved FCR, increased hemoglobin and MCV values, decreased gut pH and cecal coliform of broiler chickens.","PeriodicalId":17806,"journal":{"name":"Jurnal Ilmu Ternak dan Veteriner","volume":"25 1","pages":"182-190"},"PeriodicalIF":0.5,"publicationDate":"2020-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44321044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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