首页 > 最新文献

Jurnal Kimia Sains dan Aplikasi最新文献

英文 中文
Effect of Ascorbic Acid Concentration on Cu2O Production for Photoelectrochemical Water Splitting on Photocathode Thin Films 抗坏血酸浓度对光电阴极薄膜电解水分解Cu2O产率的影响
Pub Date : 2022-12-21 DOI: 10.14710/jksa.25.12.442-449
K. L. Rahayu, Abdul Haris, G. Gunawan
Hydrogen energy has great potential as a renewable energy source. Electrochemical water-splitting can be employed to obtain hydrogen by converting solar energy into hydrogen. In this study, Cu2O thin film electrodes have been successfully synthesized using ascorbic acid using the spin coating method. This study aimed to determine the effect of ascorbic acid in manufacturing Cu2O semiconductors as photocathodes and their activity for electrochemical water-splitting. The results indicated that ascorbate affected the photon current and onset potential of the Cu2O semiconductor. The synthesis results found that Cu2O at C1 (lower concentration than Cu2+) yielded 95.69%, and the yield for Cu2O at C2 (concentration equal to Cu2+) was 96.2%. The yield for Cu2O at C3 (concentration greater than Cu2+) was 99.82%. The photon currents generated by adding 3%, 6%, and 9% ascorbate solution were 1.18, 1.69, and 1.78 mA/cm2, respectively, at 0.3 V vs. RHE (Reversible Hydrogen Electrode). X-ray diffraction analysis revealed that the sample consisted of Cu2O C3 with an average grain size of 17.55 nm. Meanwhile, Cu2O C1 and Cu2O C2 had average grain sizes of 38.99 nm and 36.42 nm, respectively. SEM analysis showed the presence of Cu2O with a cuboid and flower-like morphology. EDX analysis showed that the samples contained elements of Cu: O, 73.97%: 26.03%; 79.89%: 20.11% and 98.43%: 1.57% respectively.
氢能作为一种可再生能源具有巨大的潜力。电化学水分解可以通过将太阳能转化为氢气来获得氢气。本研究以抗坏血酸为原料,采用旋涂法成功合成了Cu2O薄膜电极。本研究旨在确定抗坏血酸在制备Cu2O半导体光电阴极中的作用及其电化学分解水的活性。结果表明,抗坏血酸盐对Cu2O半导体的光子电流和起始电位有影响。合成结果表明,在C1(浓度低于Cu2+)时,Cu2O的产率为95.69%,在C2(浓度等于Cu2+)下,Cu2O的产率为96.2%。在C3(浓度大于Cu2+)处,Cu2O产率为99.82%。在0.3V下,添加3%、6%和9%抗坏血酸溶液产生的光子电流分别为1.18、1.69和1.78mA/cm2。RHE(可逆氢电极)。X射线衍射分析表明,样品由Cu2O C3组成,平均晶粒尺寸为17.55nm。同时,Cu2O C1和Cu2O C2的平均晶粒尺寸分别为38.99 nm和36.42 nm。SEM分析显示存在具有长方体和花状形态的Cu2O。EDX分析表明,样品中Cu含量为73.97%:26.03%;79.89%:20.11%和98.43%:1.57%。
{"title":"Effect of Ascorbic Acid Concentration on Cu2O Production for Photoelectrochemical Water Splitting on Photocathode Thin Films","authors":"K. L. Rahayu, Abdul Haris, G. Gunawan","doi":"10.14710/jksa.25.12.442-449","DOIUrl":"https://doi.org/10.14710/jksa.25.12.442-449","url":null,"abstract":"Hydrogen energy has great potential as a renewable energy source. Electrochemical water-splitting can be employed to obtain hydrogen by converting solar energy into hydrogen. In this study, Cu2O thin film electrodes have been successfully synthesized using ascorbic acid using the spin coating method. This study aimed to determine the effect of ascorbic acid in manufacturing Cu2O semiconductors as photocathodes and their activity for electrochemical water-splitting. The results indicated that ascorbate affected the photon current and onset potential of the Cu2O semiconductor. The synthesis results found that Cu2O at C1 (lower concentration than Cu2+) yielded 95.69%, and the yield for Cu2O at C2 (concentration equal to Cu2+) was 96.2%. The yield for Cu2O at C3 (concentration greater than Cu2+) was 99.82%. The photon currents generated by adding 3%, 6%, and 9% ascorbate solution were 1.18, 1.69, and 1.78 mA/cm2, respectively, at 0.3 V vs. RHE (Reversible Hydrogen Electrode). X-ray diffraction analysis revealed that the sample consisted of Cu2O C3 with an average grain size of 17.55 nm. Meanwhile, Cu2O C1 and Cu2O C2 had average grain sizes of 38.99 nm and 36.42 nm, respectively. SEM analysis showed the presence of Cu2O with a cuboid and flower-like morphology. EDX analysis showed that the samples contained elements of Cu: O, 73.97%: 26.03%; 79.89%: 20.11% and 98.43%: 1.57% respectively.","PeriodicalId":17811,"journal":{"name":"Jurnal Kimia Sains dan Aplikasi","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44491433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Interaction Mechanism of Inhibition of Palmitic Acid and α Selinene Targeting FabH and FabI Enzymes in Escherichia coli: In Silico Study 棕榈酸和αSelinene对大肠杆菌中FabH和FabI酶的相互作用机制的Silico研究
Pub Date : 2022-12-20 DOI: 10.14710/jksa.25.12.427-435
Tia Aulia, Nies Suci Suci Mulyani, M. Asy'ari
Investigation studies of the interaction mechanism of palmitic acid and α-selinene in inhibiting FabH and FabI enzymes have been studied using an in silico approach. FabH (Beta-Ketoacyl-ACP Synthase III) and FabI (Enoyl-acyl carrier protein reductase) enzymes are two enzymes that are targets for the inhibition of candidate antibacterial compounds. This study aimed to determine the strongest candidate between palmitic acid and α-selinene as an antibacterial agent for Escherichia coli. The method used in this study is a random and directed molecular docking method using the Autodock Vina program, which is integrated into PyRx 0.8 software. The results of the molecular docking simulation include the pattern and strength of interaction between the ligand and the FabI and FabH enzymes. The interaction pattern includes the cluster pattern, the ligand poses on the protein surface, and the interaction strength based on the binding affinity value. Based on the results of random docking simulation data analysis, it was shown that the majority of α-selinene occupied the position of cluster 1 of the FabI enzyme and palmitic acid in cluster 2 of the FabH enzyme. Based on the binding affinity value, palmitic acid has a weaker interaction strength on the FabH enzyme (-5.7 kcal/mol) than on the FabI enzyme (-7.1 kcal/mol). The interaction strength of α-selinene on the FabI enzyme (-7.3 kcal/mol) was stronger than that of the FabH enzyme (-6.9 kcal/mol). The interaction strength of α-selinene in both FabI and FabH enzymes was greater than that of palmitic acid. α-selinene is projected to have a better potential as an antibacterial agent against Escherichia coli than palmitic acid based on its greater interaction strength.
采用计算机模拟方法研究了棕榈酸和α-selinene抑制FabH和FabI酶的相互作用机制。FabH(β-酮酰基ACP合成酶III)和FabI(烯酰基载体蛋白还原酶)是抑制候选抗菌化合物的两种酶。本研究旨在确定棕榈酸和α-selinene之间最强的候选物,作为大肠杆菌的抗菌剂。本研究中使用的方法是一种使用Autodock Vina程序的随机和定向分子对接方法,该程序集成到PyRx 0.8软件中。分子对接模拟的结果包括配体与FabI和FabH酶之间相互作用的模式和强度。相互作用模式包括簇模式、配体在蛋白质表面的姿态以及基于结合亲和力值的相互作用强度。基于随机对接模拟数据分析的结果表明,大多数α-selinene占据FabI酶簇1的位置,棕榈酸占据FabH酶簇2中的位置。基于结合亲和力值,棕榈酸对FabH酶的相互作用强度(-5.7kcal/mol)比对FabI酶的相互作用力强度(-7.1kcal/mol)弱。α-selinee对FabI酶的相互作用强度(-7.3kcal/mol)强于FabH酶的作用强度(-6.9kcal/mol。α-selinene在FabI和FabH酶中的相互作用强度均大于棕榈酸。α-selinene因其更大的相互作用强度而被认为比棕榈酸具有更好的抗大肠杆菌潜力。
{"title":"Interaction Mechanism of Inhibition of Palmitic Acid and α Selinene Targeting FabH and FabI Enzymes in Escherichia coli: In Silico Study","authors":"Tia Aulia, Nies Suci Suci Mulyani, M. Asy'ari","doi":"10.14710/jksa.25.12.427-435","DOIUrl":"https://doi.org/10.14710/jksa.25.12.427-435","url":null,"abstract":"Investigation studies of the interaction mechanism of palmitic acid and α-selinene in inhibiting FabH and FabI enzymes have been studied using an in silico approach. FabH (Beta-Ketoacyl-ACP Synthase III) and FabI (Enoyl-acyl carrier protein reductase) enzymes are two enzymes that are targets for the inhibition of candidate antibacterial compounds. This study aimed to determine the strongest candidate between palmitic acid and α-selinene as an antibacterial agent for Escherichia coli. The method used in this study is a random and directed molecular docking method using the Autodock Vina program, which is integrated into PyRx 0.8 software. The results of the molecular docking simulation include the pattern and strength of interaction between the ligand and the FabI and FabH enzymes. The interaction pattern includes the cluster pattern, the ligand poses on the protein surface, and the interaction strength based on the binding affinity value. Based on the results of random docking simulation data analysis, it was shown that the majority of α-selinene occupied the position of cluster 1 of the FabI enzyme and palmitic acid in cluster 2 of the FabH enzyme. Based on the binding affinity value, palmitic acid has a weaker interaction strength on the FabH enzyme (-5.7 kcal/mol) than on the FabI enzyme (-7.1 kcal/mol). The interaction strength of α-selinene on the FabI enzyme (-7.3 kcal/mol) was stronger than that of the FabH enzyme (-6.9 kcal/mol). The interaction strength of α-selinene in both FabI and FabH enzymes was greater than that of palmitic acid. α-selinene is projected to have a better potential as an antibacterial agent against Escherichia coli than palmitic acid based on its greater interaction strength.","PeriodicalId":17811,"journal":{"name":"Jurnal Kimia Sains dan Aplikasi","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43198559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effect of Storage of Yellow Pigment from Halophilic Bacillus clausii J1G-0%B on Antioxidant Activity 克氏嗜盐芽孢杆菌jg1 -0% b黄色素贮藏对抗氧化活性的影响
Pub Date : 2022-12-20 DOI: 10.14710/jksa.25.11.399-404
Galuh Dwi Arum, M. Asy'ari, N. Mulyani
Oxidative stress is a state of excess free radicals in the body, which results in increased oxidation processes in the body’s cells and causes damage. In previous studies, one way to neutralize this situation was found, namely with halophilic bacteria. Halophilic bacteria can live at a salt content of 2% to 30%. These bacteria can produce various kinds of pigments for self-defense from extreme environments, which function as immunomodulatory compounds and have antioxidant activity. The antioxidant activity of the yellow pigment halophilic bacteria Bacillus clausii J1G-0%B using the DPPH method showed that the effect of light and air on antioxidant activity was 17.88% inhibition in the crude extract and 14.24% inhibition at 1600 ppm. Antioxidant activity decreased by 17.13% under the influence of air, 1.89% under the influence of light, and 28.58% under the influence of air and light. FTIR analysis under the influence of air experienced a decrease in the peak of the CO carbonyl group of 1739.29 cm-1 and an increase in the alkane C-H group of 1369.52 cm-1, while under the influence of light and open conditions, the carbonyl CO group experienced an increase of 1739.50 cm-1 and 1739.46 cm-1 and the increase in alkane C-H groups 1371.41 cm-1 and 1369.53 cm- 1.
氧化应激是体内自由基过剩的一种状态,它会导致体内细胞氧化过程增加,并造成损害。在以前的研究中,发现了一种中和这种情况的方法,即嗜盐细菌。嗜盐细菌可以在含盐量为2%至30%的环境中生存。这些细菌可以产生多种色素,作为免疫调节化合物,具有抗氧化活性,可以抵御极端环境。DPPH法测定黄色色素嗜盐细菌克氏芽孢杆菌J1G-0%B的抗氧化活性,结果表明,光和空气对其抗氧化活性的影响在粗提物中为17.88%,在1600 ppm时为14.24%。空气影响下抗氧化活性降低17.13%,光影响下降低1.89%,空气和光共同作用下降低28.58%。空气影响下,CO羰基峰1739.29 cm-1降低,烷烃C-H基团峰1369.52 cm-1升高;光照和开放条件下,CO羰基峰1739.50 cm-1和1739.46 cm-1升高,烷烃C-H基团峰1371.41 cm-1和1369.53 cm-1升高。
{"title":"Effect of Storage of Yellow Pigment from Halophilic Bacillus clausii J1G-0%B on Antioxidant Activity","authors":"Galuh Dwi Arum, M. Asy'ari, N. Mulyani","doi":"10.14710/jksa.25.11.399-404","DOIUrl":"https://doi.org/10.14710/jksa.25.11.399-404","url":null,"abstract":"Oxidative stress is a state of excess free radicals in the body, which results in increased oxidation processes in the body’s cells and causes damage. In previous studies, one way to neutralize this situation was found, namely with halophilic bacteria. Halophilic bacteria can live at a salt content of 2% to 30%. These bacteria can produce various kinds of pigments for self-defense from extreme environments, which function as immunomodulatory compounds and have antioxidant activity. The antioxidant activity of the yellow pigment halophilic bacteria Bacillus clausii J1G-0%B using the DPPH method showed that the effect of light and air on antioxidant activity was 17.88% inhibition in the crude extract and 14.24% inhibition at 1600 ppm. Antioxidant activity decreased by 17.13% under the influence of air, 1.89% under the influence of light, and 28.58% under the influence of air and light. FTIR analysis under the influence of air experienced a decrease in the peak of the CO carbonyl group of 1739.29 cm-1 and an increase in the alkane C-H group of 1369.52 cm-1, while under the influence of light and open conditions, the carbonyl CO group experienced an increase of 1739.50 cm-1 and 1739.46 cm-1 and the increase in alkane C-H groups 1371.41 cm-1 and 1369.53 cm- 1.","PeriodicalId":17811,"journal":{"name":"Jurnal Kimia Sains dan Aplikasi","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66999691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effect of pH on the Synthesis of Silica Sol-Gel Tetraethylorthosilicate-Trimethylchlorosilan (TEOS-TMCS) pH对硅溶胶-凝胶-四乙基硅酸-三甲基氯硅兰(TEOS-TMCS)合成的影响
Pub Date : 2022-12-20 DOI: 10.14710/jksa.25.11.394-398
Artha Monica Andini, C. Azmiyawati, A. Darmawan
Sol-gel synthesis of silica employing the co-precursor trimethylchlorosilane (TMCS) and the precursor tetraethylorthosilicate (TEOS) was accomplished. The purpose of this investigation was to ascertain how pH affected the properties of synthetic silica. The synthesized hydrophobic silica was characterized using FTIR, TGA, and GSA to determine the effect of pH adjustment on the functional group characters, thermal properties, and pore morphology. The ratio between TEOS/TMCS was fixed at 75:25 and varied the pH (4, 6, 7, 8, and 10) and the calcination temperature (without calcination, 300℃ and 500℃). FTIR analysis showed that the number of C-H and Si-OH groups in the xerogel decreased with increasing calcination temperature. Xerogel formed at pH 6 provides the highest thermal stability among other pHs. The results from the BET analysis revealed that changes in pH directly affect the physical characteristics of the surface, making the resulting gel network less rigid and more susceptible to shrinkage of pore volume and diameter in the atmosphere. Meanwhile, in an alkaline medium, continuous condensation will occur so that the pore diameter and volume decrease. The high pore diameter and volume imply that pH 7 is ideal for preparing xerogels.
以三甲基氯硅烷(TMCS)和四乙基硅酸盐(TEOS)为前驱体,完成了溶胶-凝胶法合成二氧化硅的研究。本研究的目的是确定pH值如何影响合成二氧化硅的性能。利用红外光谱(FTIR)、热重分析(TGA)和GSA对合成的疏水二氧化硅进行了表征,以确定pH调节对其官能团特征、热性能和孔隙形态的影响。TEOS/TMCS的比例固定为75:25,改变pH(4、6、7、8、10)和煅烧温度(未煅烧、300℃和500℃)。FTIR分析表明,随着煅烧温度的升高,干凝胶中C-H和Si-OH基团的数量减少。pH值为6时形成的干凝胶在其他pH值中具有最高的热稳定性。BET分析的结果表明,pH值的变化直接影响了表面的物理特性,使凝胶网络的刚性降低,更容易受到大气中孔隙体积和直径收缩的影响。同时,在碱性介质中,会发生连续的冷凝,使孔径和体积减小。高孔径和大体积意味着pH为7是制备干凝胶的理想条件。
{"title":"Effect of pH on the Synthesis of Silica Sol-Gel Tetraethylorthosilicate-Trimethylchlorosilan (TEOS-TMCS)","authors":"Artha Monica Andini, C. Azmiyawati, A. Darmawan","doi":"10.14710/jksa.25.11.394-398","DOIUrl":"https://doi.org/10.14710/jksa.25.11.394-398","url":null,"abstract":"Sol-gel synthesis of silica employing the co-precursor trimethylchlorosilane (TMCS) and the precursor tetraethylorthosilicate (TEOS) was accomplished. The purpose of this investigation was to ascertain how pH affected the properties of synthetic silica. The synthesized hydrophobic silica was characterized using FTIR, TGA, and GSA to determine the effect of pH adjustment on the functional group characters, thermal properties, and pore morphology. The ratio between TEOS/TMCS was fixed at 75:25 and varied the pH (4, 6, 7, 8, and 10) and the calcination temperature (without calcination, 300℃ and 500℃). FTIR analysis showed that the number of C-H and Si-OH groups in the xerogel decreased with increasing calcination temperature. Xerogel formed at pH 6 provides the highest thermal stability among other pHs. The results from the BET analysis revealed that changes in pH directly affect the physical characteristics of the surface, making the resulting gel network less rigid and more susceptible to shrinkage of pore volume and diameter in the atmosphere. Meanwhile, in an alkaline medium, continuous condensation will occur so that the pore diameter and volume decrease. The high pore diameter and volume imply that pH 7 is ideal for preparing xerogels.","PeriodicalId":17811,"journal":{"name":"Jurnal Kimia Sains dan Aplikasi","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67000145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Synthesis of Lead Oxide Using the Oxidizing Agent (NH4)2S2O8 with Variaous Amounts of NaOH for Decolorization of Remazol Black B Solution 用氧化剂(NH4)2S2O8和不同量的NaOH合成氧化铅对雷马唑黑B溶液的脱色
Pub Date : 2022-12-19 DOI: 10.14710/jksa.25.12.436-441
Meiske Erdinarini Anggita, Abdul Haris, D. S. Widodo
In this study, the lead oxide has been successfully synthesized using oxidizing agents (NH4)2S2O8 and applied to decolorize the Remazole Black B (RBB) solution. Lead oxide was synthesized by the batch method through the mechanism of reduction-oxidation and characterized using SEM-EDX and XRD. Decolorizing RBB solution was performed using a modified Fenton method. This study aims to determine the optimum performance of RBB decolorization from lead oxide. The results showed that the crystal formed contains Pb and O. Pb oxide obtained was α-PBO2 with the orthorhombic crystal system. Based on characterization, the lead oxide obtained has a surface morphology of coral flowers-like. Synthesis utilizing equilibrium composition (1:2) and a drying temperature of 100°C yielded Pb oxide, which has a better potential as a modifier in RBB decolorization with a decolorization percentage of 80.07%. The process was then carried out for advanced synthesis less and excess of molar variations. The ratio of lead oxide: NaOH (1:4) showed optimum performance in decolorizing RBB with a decolorization percentage of 80.07 %.
本研究以氧化剂(NH4)2S2O8为原料成功合成了氧化铅,并将其用于雷唑黑B (RBB)溶液的脱色。采用还原-氧化机理,采用间歇法合成氧化铅,并用SEM-EDX和XRD对其进行了表征。采用改进的Fenton法对RBB溶液进行脱色。本研究旨在确定RBB脱色氧化铅的最佳性能。结果表明,形成的晶体中含有Pb和o,得到的氧化铅为α-PBO2,具有正交晶系。根据表征,所得氧化铅具有珊瑚花状的表面形态。在平衡组成(1:2)和干燥温度为100℃的条件下合成得到氧化铅,氧化铅作为RBB脱色改性剂具有较好的潜力,脱色率为80.07%。然后进行了先进的合成较少和过量的摩尔变化。氧化铅:氢氧化钠(1:4)对RBB的脱色效果最佳,脱色率为80.07%。
{"title":"Synthesis of Lead Oxide Using the Oxidizing Agent (NH4)2S2O8 with Variaous Amounts of NaOH for Decolorization of Remazol Black B Solution","authors":"Meiske Erdinarini Anggita, Abdul Haris, D. S. Widodo","doi":"10.14710/jksa.25.12.436-441","DOIUrl":"https://doi.org/10.14710/jksa.25.12.436-441","url":null,"abstract":"In this study, the lead oxide has been successfully synthesized using oxidizing agents (NH4)2S2O8 and applied to decolorize the Remazole Black B (RBB) solution. Lead oxide was synthesized by the batch method through the mechanism of reduction-oxidation and characterized using SEM-EDX and XRD. Decolorizing RBB solution was performed using a modified Fenton method. This study aims to determine the optimum performance of RBB decolorization from lead oxide. The results showed that the crystal formed contains Pb and O. Pb oxide obtained was α-PBO2 with the orthorhombic crystal system. Based on characterization, the lead oxide obtained has a surface morphology of coral flowers-like. Synthesis utilizing equilibrium composition (1:2) and a drying temperature of 100°C yielded Pb oxide, which has a better potential as a modifier in RBB decolorization with a decolorization percentage of 80.07%. The process was then carried out for advanced synthesis less and excess of molar variations. The ratio of lead oxide: NaOH (1:4) showed optimum performance in decolorizing RBB with a decolorization percentage of 80.07 %.","PeriodicalId":17811,"journal":{"name":"Jurnal Kimia Sains dan Aplikasi","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46177656","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Isolation and Antioxidant Activity of Flavonoid Compound in Ethanolic Extract of Celery Leaves (Apium graveolens L.) 芹菜叶乙醇提取物中黄酮类化合物的分离及抗氧化活性研究
Pub Date : 2022-12-18 DOI: 10.14710/jksa.25.12.450-455
A. Kholieqoh, K. Anam, D. Kusrini
Celery (Apium graveolens L.) is a plant that belongs to the Apiaceae family and is widely used as a medical plant for low blood pressure, heart tonic, and to prevent cardiovascular disease. This study aims to obtain flavonoid compounds, identify, and test the antioxidant activity of flavonoid compounds and crude extracts from celery leaves. The research procedures consisted of four steps, the first of which was a preliminary test. The second step involved isolating and separating flavonoid components by vacuum liquid chromatography, gravitational column chromatography, and preparative thin layer chromatography. The third step was to identify flavonoid compounds using reagent shift, FTIR, and LCMS/MS. And finally, antioxidant activity was evaluated using the DPPH method. The preliminary test result showed that the ethanolic extract of leaves and stems had a total flavonoid content of 13.99 and 2.46 mg QE/g of dry weight. Both dry leaves and crude extract of celery leaves contained alkaloids, saponin, flavonoid, tannin, quinone, and steroid/triterpenoid, as determined by phytochemical screening. Isolation and separation of flavonoids yielded A2.I and A2.II isolates, with respective weights of 8 mg and 14 mg. Identification of flavonoid compounds using reagent shift showed that two isolates have the basic structure of the flavone group. A2.I isolate had OH groups at 4’, 5, and 7, while A2.II isolate had OH groups at 3’, 4’, 5, and 7. The FTIR analysis revealed that both compounds contain functional groups, including O-H, C=O, C=C aromatic, C-O ether, C-O alcohol, and C-H aromatic ring. According to LCMS/MS analysis, the molecular weights of A2.I and A2.II were 270 g/mol and 286 g/mol, respectively. All of the identification methods for isolates showed that A2.I was apigenin and A2.II was luteolin. Antioxidant activity by DPPH method for a viscous extract of celery leaves, A2.I, and A2.II were 775.41, 288.95, and 184.35 µg/mL, respectively.
芹菜(Apium graveolens L.)是一种属于芹菜科的植物,被广泛用作降低血压、滋补心脏和预防心血管疾病的药用植物。本研究旨在从芹菜叶中提取类黄酮化合物,并对类黄酮化合物及粗提物的抗氧化活性进行鉴定和测试。研究程序包括四个步骤,第一步是初步测试。第二步采用真空液相色谱法、重力柱色谱法和制备薄层色谱法分离黄酮类成分。第三步采用试剂移位、FTIR和LCMS/MS鉴定黄酮类化合物。最后用DPPH法评价其抗氧化活性。初步试验结果表明,叶和茎的乙醇提取物总黄酮含量分别为13.99和2.46 mg QE/g干重。芹菜干叶和粗提物均含有生物碱、皂苷、黄酮类、单宁、醌类和甾体/三萜。黄酮类化合物的分离得到A2。I和A2。2个分离株,重量分别为8 mg和14 mg。用试剂移位法对黄酮类化合物进行鉴定,发现两株分离物均具有黄酮基的基本结构。A2。我分离出4 ' 5 '和7号羟基,而A2。II型分离物在3 '、4 '、5和7处均含有OH基团。FTIR分析表明,两种化合物均含有O- h、C=O、C=C芳、C-O醚、C-O醇和C- h芳环等官能团。经LCMS/MS分析,A2的分子量。I和A2。II分别为270 g/mol和286 g/mol。所有分离物的鉴定方法均显示A2。我是芹菜素和A2。第二种是木犀草素。DPPH法测定芹菜叶黏性提取物的抗氧化活性。I和A2。II分别为775.41、288.95和184.35µg/mL。
{"title":"Isolation and Antioxidant Activity of Flavonoid Compound in Ethanolic Extract of Celery Leaves (Apium graveolens L.)","authors":"A. Kholieqoh, K. Anam, D. Kusrini","doi":"10.14710/jksa.25.12.450-455","DOIUrl":"https://doi.org/10.14710/jksa.25.12.450-455","url":null,"abstract":"Celery (Apium graveolens L.) is a plant that belongs to the Apiaceae family and is widely used as a medical plant for low blood pressure, heart tonic, and to prevent cardiovascular disease. This study aims to obtain flavonoid compounds, identify, and test the antioxidant activity of flavonoid compounds and crude extracts from celery leaves. The research procedures consisted of four steps, the first of which was a preliminary test. The second step involved isolating and separating flavonoid components by vacuum liquid chromatography, gravitational column chromatography, and preparative thin layer chromatography. The third step was to identify flavonoid compounds using reagent shift, FTIR, and LCMS/MS. And finally, antioxidant activity was evaluated using the DPPH method. The preliminary test result showed that the ethanolic extract of leaves and stems had a total flavonoid content of 13.99 and 2.46 mg QE/g of dry weight. Both dry leaves and crude extract of celery leaves contained alkaloids, saponin, flavonoid, tannin, quinone, and steroid/triterpenoid, as determined by phytochemical screening. Isolation and separation of flavonoids yielded A2.I and A2.II isolates, with respective weights of 8 mg and 14 mg. Identification of flavonoid compounds using reagent shift showed that two isolates have the basic structure of the flavone group. A2.I isolate had OH groups at 4’, 5, and 7, while A2.II isolate had OH groups at 3’, 4’, 5, and 7. The FTIR analysis revealed that both compounds contain functional groups, including O-H, C=O, C=C aromatic, C-O ether, C-O alcohol, and C-H aromatic ring. According to LCMS/MS analysis, the molecular weights of A2.I and A2.II were 270 g/mol and 286 g/mol, respectively. All of the identification methods for isolates showed that A2.I was apigenin and A2.II was luteolin. Antioxidant activity by DPPH method for a viscous extract of celery leaves, A2.I, and A2.II were 775.41, 288.95, and 184.35 µg/mL, respectively.","PeriodicalId":17811,"journal":{"name":"Jurnal Kimia Sains dan Aplikasi","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48652407","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Isolation, Identification, and Antioxidant Activity of Flavonoid Compounds in the Ethanol Extract in Bandotan Leaves (Ageratum conyzoides) 板多坦叶乙醇提取物中黄酮类化合物的分离、鉴定及抗氧化活性研究
Pub Date : 2022-12-13 DOI: 10.14710/jksa.25.12.456-466
Siti Mutingatun, E. Fachriyah, D. Kusrini
Isolation of flavonoid compounds from the ethanol extract of Bandotan leaves (Ageratum conyzoides L.) has been successfully conducted. The structure of flavonoid isolates was identified using UV-Vis spectrophotometry with the addition of shear reagents and FTIR. In addition, the antioxidant activity of the isolates was determined and studied using the DPPH (2,2-Diphenyl-1-picrylhydrazyl) method. This study aimed to isolate, identify, and test the antioxidant activity of flavonoid compounds from Bandotan leaves. An antioxidant activity test was carried out on ethanol extract, and flavonoid isolates using the DPPH method. Based on this research, Bandotan leaf ethanol extract yielded 13.964%. The results of the phytochemical screening test revealed that the leaf powder and ethanol extract of Bandotan leaves contained alkaloids, flavonoids, saponins, tannins, quinones, steroids, and triterpenoids. The total flavonoid content of the ethanol extract of Bandotan leaves was 129.27 mg EQ/g extract. Identification of flavonoid isolates using a UV-Vis spectrophotometer showed that flavonoid isolates belong to the flavanone compound class with maximum absorption at a wavelength of 315 nm (band I) and 280 nm (band II). FTIR analysis showed that the flavonoid isolates had functional groups O-H, C-H aromatics, C-H alkanes, C=O, C=C aromatics, C-O ethers, C-O alcohols, and substituted aromatic rings. Identification of the structure of flavonoid isolates with a spectrophotometer with the addition of shear reagent and FTIR, presumably that the isolate was a 4’-hydroxy flavanone compound. The antioxidant activity of ethanol extract showed moderate antioxidant activity (IC50 118.19 µg/mL) and was classified as very weak (IC50 1185.5 µg/mL).
本文成功地从板多坦(Ageratum conyzoides L.)叶乙醇提取物中分离出黄酮类化合物。采用紫外-可见分光光度法,添加剪切试剂和FTIR对分离的黄酮类化合物进行结构鉴定。此外,采用DPPH(2,2-二苯基-1-苦味酰肼)法测定了分离物的抗氧化活性。本研究旨在分离、鉴定和检测板多坦叶中黄酮类化合物的抗氧化活性。采用DPPH法对乙醇提取物和黄酮类分离物进行抗氧化活性试验。在此基础上,板多坦叶乙醇提取物提取率为13.964%。植物化学筛选试验结果表明,班度坦叶粉和乙醇提取物中含有生物碱、黄酮类、皂苷、单宁、醌类、甾体和三萜。板多坦叶乙醇提取物总黄酮含量为129.27 mg EQ/g提取物。紫外-可见分光光度计对分离的类黄酮进行鉴定,其最大吸收波长为315 nm(波段I)和280 nm(波段II)。FTIR分析表明,分离的类黄酮具有O- h、C- h芳烃、C- h烷烃、C=O、C=C芳烃、C-O醚、C-O醇和取代芳环。用分光光度计加剪切剂和FTIR对分离物的结构进行了鉴定,推测分离物为4′-羟基黄酮化合物。乙醇提取物的抗氧化活性为中等(IC50为118.19µg/mL),极弱(IC50为1185.5µg/mL)。
{"title":"Isolation, Identification, and Antioxidant Activity of Flavonoid Compounds in the Ethanol Extract in Bandotan Leaves (Ageratum conyzoides)","authors":"Siti Mutingatun, E. Fachriyah, D. Kusrini","doi":"10.14710/jksa.25.12.456-466","DOIUrl":"https://doi.org/10.14710/jksa.25.12.456-466","url":null,"abstract":"Isolation of flavonoid compounds from the ethanol extract of Bandotan leaves (Ageratum conyzoides L.) has been successfully conducted. The structure of flavonoid isolates was identified using UV-Vis spectrophotometry with the addition of shear reagents and FTIR. In addition, the antioxidant activity of the isolates was determined and studied using the DPPH (2,2-Diphenyl-1-picrylhydrazyl) method. This study aimed to isolate, identify, and test the antioxidant activity of flavonoid compounds from Bandotan leaves. An antioxidant activity test was carried out on ethanol extract, and flavonoid isolates using the DPPH method. Based on this research, Bandotan leaf ethanol extract yielded 13.964%. The results of the phytochemical screening test revealed that the leaf powder and ethanol extract of Bandotan leaves contained alkaloids, flavonoids, saponins, tannins, quinones, steroids, and triterpenoids. The total flavonoid content of the ethanol extract of Bandotan leaves was 129.27 mg EQ/g extract. Identification of flavonoid isolates using a UV-Vis spectrophotometer showed that flavonoid isolates belong to the flavanone compound class with maximum absorption at a wavelength of 315 nm (band I) and 280 nm (band II). FTIR analysis showed that the flavonoid isolates had functional groups O-H, C-H aromatics, C-H alkanes, C=O, C=C aromatics, C-O ethers, C-O alcohols, and substituted aromatic rings. Identification of the structure of flavonoid isolates with a spectrophotometer with the addition of shear reagent and FTIR, presumably that the isolate was a 4’-hydroxy flavanone compound. The antioxidant activity of ethanol extract showed moderate antioxidant activity (IC50 118.19 µg/mL) and was classified as very weak (IC50 1185.5 µg/mL).","PeriodicalId":17811,"journal":{"name":"Jurnal Kimia Sains dan Aplikasi","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44620572","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effect of Infiltration Ce0.8Sm0.2O1.9 Against Double Perovskite Performance LaBa0.5Sr0.5Co2O5+δ as IT-SOFC Cathode 渗透Ce0.8Sm0.2O1.9对LaBa0.5Sr0.5Co2O5+δ双钙钛矿IT-SOFC阴极性能的影响
Pub Date : 2022-12-13 DOI: 10.14710/jksa.25.10.346-351
A. Subardi, Yen‐Pei Fu
Modifying the sample surface by infiltration technique using Ce0.8Sm0.2O1.9 (SDC) electrolyte has been done to increase the catalytic activity of the LaBa0.5Sr0.5Co2O5+δ (LBSC) cathode. The cathode powder structure was evaluated using X-ray diffraction (XRD) at room temperature, and the LBSC cathode microstructure was analyzed using scanning electron microscopy (SEM). The electrical conductivity of the LBSC cathode was tested using the four-probe DC method. Symmetrical cells were tested using a potentiostat Voltalab PGZ 301 and a digital source meter Keithley 2420. LBSC powder was discovered to have a tetragonal structure (space group: P4/mmm) with lattice parameters of a = 3.86253 Å, c = 7.73438 Å, and V = 115.338 Å. From the SEM image, the LBSC cathode has homogeneous, dense, and highly porous grains. The electrical conductivity showed metallic behavior, gradually decreasing from 167 S.cm-1 at 300℃ to 105 S.cm-1 at 800℃. A significant increase in current density (io) of 275% occurred at 800℃ from 154.10 mA.cm−2 (pure LBSC) to 577.86 mA.cm−2 (LBSC+0.5M SDC). The activation energy value (Ea) of symmetrical cells was determined using electrochemical impedance spectroscopy (EIS), low-field (LF), and high-field (HF) techniques. The activation energy of the LBSC+0.5 M SDC specimen was 47.9 kJ mol-1 or 79.4% lower than the activation energy of the LBSC cathode specimen without infiltration at atmospheric pressure of 0.03 atm. These results indicate that SDC infiltration of the LBSC cathode can reduce the activation energy of the significant. The cathode membrane adheres quite well to the electrolyte membrane, the cathode porosity varies in the range of 1–4 µm, and the grain size is 0.1–1.5 µm.
使用Ce0.8Sm0.2O1.9(SDC)电解质通过渗透技术对样品表面进行改性,以提高LaBa0.5Sr0.5Co2O5+δ(LBSC)阴极的催化活性。在室温下使用X射线衍射(XRD)评估阴极粉末结构,并使用扫描电子显微镜(SEM)分析LBSC阴极的微观结构。使用四探针直流法测试LBSC阴极的电导率。使用恒电位仪Voltalab PGZ 301和数字源计Keithley 2420测试对称电池。发现LBSC粉末具有四方结构(空间群:P4/mmm),晶格参数为a=3.86253Å, c=7.73438Å, V=115.338Å. 从SEM图像来看,LBSC阴极具有均匀、致密和高度多孔的晶粒。电导率表现为金属行为,从300℃时的167S.cm-1逐渐降低到800℃时的105S.cm-1。在800℃时,电流密度(io)显著增加了275%,从154.10 mA.cm−2(纯LBSC)增加到577.86 mA.cm–2(LBSC+0.5M SDC)。使用电化学阻抗谱(EIS)、低场(LF)和高场(HF)技术测定对称电池的活化能值(Ea)。LBSC+0.5M SDC样品的活化能为47.9kJ mol-1,比在0.03atm的大气压下没有渗透的LBSC阴极样品的活化能量低79.4%。这些结果表明,LBSC阴极的SDC渗透可以显著降低其活化能。阴极膜与电解质膜粘附良好,阴极孔隙率在1–4µm范围内变化,晶粒尺寸为0.1–1.5µm。
{"title":"Effect of Infiltration Ce0.8Sm0.2O1.9 Against Double Perovskite Performance LaBa0.5Sr0.5Co2O5+δ as IT-SOFC Cathode","authors":"A. Subardi, Yen‐Pei Fu","doi":"10.14710/jksa.25.10.346-351","DOIUrl":"https://doi.org/10.14710/jksa.25.10.346-351","url":null,"abstract":"Modifying the sample surface by infiltration technique using Ce0.8Sm0.2O1.9 (SDC) electrolyte has been done to increase the catalytic activity of the LaBa0.5Sr0.5Co2O5+δ (LBSC) cathode. The cathode powder structure was evaluated using X-ray diffraction (XRD) at room temperature, and the LBSC cathode microstructure was analyzed using scanning electron microscopy (SEM). The electrical conductivity of the LBSC cathode was tested using the four-probe DC method. Symmetrical cells were tested using a potentiostat Voltalab PGZ 301 and a digital source meter Keithley 2420. LBSC powder was discovered to have a tetragonal structure (space group: P4/mmm) with lattice parameters of a = 3.86253 Å, c = 7.73438 Å, and V = 115.338 Å. From the SEM image, the LBSC cathode has homogeneous, dense, and highly porous grains. The electrical conductivity showed metallic behavior, gradually decreasing from 167 S.cm-1 at 300℃ to 105 S.cm-1 at 800℃. A significant increase in current density (io) of 275% occurred at 800℃ from 154.10 mA.cm−2 (pure LBSC) to 577.86 mA.cm−2 (LBSC+0.5M SDC). The activation energy value (Ea) of symmetrical cells was determined using electrochemical impedance spectroscopy (EIS), low-field (LF), and high-field (HF) techniques. The activation energy of the LBSC+0.5 M SDC specimen was 47.9 kJ mol-1 or 79.4% lower than the activation energy of the LBSC cathode specimen without infiltration at atmospheric pressure of 0.03 atm. These results indicate that SDC infiltration of the LBSC cathode can reduce the activation energy of the significant. The cathode membrane adheres quite well to the electrolyte membrane, the cathode porosity varies in the range of 1–4 µm, and the grain size is 0.1–1.5 µm.","PeriodicalId":17811,"journal":{"name":"Jurnal Kimia Sains dan Aplikasi","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44737344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Optimization of Cellulase Production by Aspergillus niger InaCC F506 in Solid-State Fermentation of Tofu Dreg 黑曲霉InaCC F506在豆腐渣固态发酵中产纤维素酶的优化
Pub Date : 2022-12-12 DOI: 10.14710/jksa.25.11.419-426
Talisia Kresna Shih, A. L. Aminin, N. Mulyani
Indonesia has a growing demand for cellulase enzymes; however, 99% of the enzymes are imported from other countries. Aspergillus niger is well recognized for using the widely accessible tofu by-product, often known as tofu dreg, as a growth medium for synthesizing cellulase enzymes. This study aims to optimize the production of cellulase enzymes by Aspergillus niger InaCC F506 using tofu dregs as a substrate through the Solid-State Fermentation (SSF) method by varying the additives. The results showed that the E fermentation system with the composition of urea 0.5%; CMC 0.5%; KH2PO4 0.2%; MgSO4.7H2O 0.2% produced the highest cellulase enzymes from the tofu dregs substrate. The highest cellulase enzyme activity was at a fraction of ammonium sulfate saturation level of 40-60%. The optimum condition of enzyme activity was observed at pH 5 with an activity of 33 x 10-4 Units/mg protein and at 30℃ with an activity of 31 x 10-4 Units/mg protein.
印度尼西亚对纤维素酶的需求不断增长;然而,99%的酶是从其他国家进口的。黑曲霉是公认的广泛使用豆腐副产品,通常被称为豆腐渣,作为合成纤维素酶的生长培养基。本研究旨在通过改变添加剂,以豆渣为底物,通过固态发酵(SSF)法优化黑曲霉InaCC F506生产纤维素酶。结果表明,E发酵体系中尿素的组成为0.5%;CMC 0.5%;KH2PO4 0.2%;从豆渣底物中,MgSO4.7H2O 0.2%产生的纤维素酶最高。纤维素酶活性在硫酸铵饱和水平40 ~ 60%时最高。在pH为5时,酶活性为33 × 10-4 Units/mg蛋白;在30℃时,酶活性为31 × 10-4 Units/mg蛋白。
{"title":"Optimization of Cellulase Production by Aspergillus niger InaCC F506 in Solid-State Fermentation of Tofu Dreg","authors":"Talisia Kresna Shih, A. L. Aminin, N. Mulyani","doi":"10.14710/jksa.25.11.419-426","DOIUrl":"https://doi.org/10.14710/jksa.25.11.419-426","url":null,"abstract":"Indonesia has a growing demand for cellulase enzymes; however, 99% of the enzymes are imported from other countries. Aspergillus niger is well recognized for using the widely accessible tofu by-product, often known as tofu dreg, as a growth medium for synthesizing cellulase enzymes. This study aims to optimize the production of cellulase enzymes by Aspergillus niger InaCC F506 using tofu dregs as a substrate through the Solid-State Fermentation (SSF) method by varying the additives. The results showed that the E fermentation system with the composition of urea 0.5%; CMC 0.5%; KH2PO4 0.2%; MgSO4.7H2O 0.2% produced the highest cellulase enzymes from the tofu dregs substrate. The highest cellulase enzyme activity was at a fraction of ammonium sulfate saturation level of 40-60%. The optimum condition of enzyme activity was observed at pH 5 with an activity of 33 x 10-4 Units/mg protein and at 30℃ with an activity of 31 x 10-4 Units/mg protein.","PeriodicalId":17811,"journal":{"name":"Jurnal Kimia Sains dan Aplikasi","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48770682","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Green Synthesis of Copper Nanoparticles Using Red Dragon Fruit (Hylocereus polyrhizus) Extract and Its Antibacterial Activity for Liquid Disinfectant 红火果提取物绿色合成纳米铜及其对液体消毒剂的抑菌活性
Pub Date : 2022-12-12 DOI: 10.14710/jksa.25.10.352-361
Cindy Agriningsih Haruna, Widy Aprillia Malik, Muhammad Yaqub Syamsul Rijal, A. H. Watoni, L. Ramadhan
The effort of copper nanoparticle exploration for antibacterial cleaning agents needs to prevent infection or contamination of microorganisms such as bacteria and viruses due to its potential characteristic as an environmentally benign material. The synthesis of copper nanoparticles using 0.02 M Cu(NO3)2 as a precursor and red dragon fruit extract as a bioreduction agent has been conducted. The extraction process of red dragon fruit was done by the maceration method using methanol as solvent. Copper nanoparticles were prepared through green chemistry with the reduction method. The compound in red dragon fruit extract reduced Cu2+ to Cu. The experimental nanoparticle synthesis method was performed with a combination of red dragon fruit extract and Cu(NO3)2 solution mixed with a volume ratio of bioreductor extract toward Cu2+ precursor variation of 1:1, 1:2, 1:3, 1:4, 1:5, 1:6, 1:7, 1:8, and 1:9. The mixtures were stirred using a magnetic stirrer for 30 minutes and then incubated. The samples were characterized using UV-Vis (Ultraviolet-Visible), FTIR (Fourier Transform Infrared), and a digital microscope to observe morphology. The observation continued to assess its antibacterial activity and potency as an active disinfectant. UV-Vis analysis showed that the absorbance value for 30 minutes tended to be stable. Particle size estimation showed the smallest size of 122.12 nm at a volume ratio of 1:9. Functional group analysis using FTIR showed a shift in wavenumber between dragon fruit extract and copper nanoparticle (CuNPs), indicating a functional group interaction. The results of morphological analysis using a digital microscope showed that the particles with fine powder granules were produced with the composition of fruit extract: Cu(NO3)2 of 1:9. Antibacterial activity against Staphylococcus aureus and Escherichia coli indicated that the composition of Cu-nanoparticles of 1:9 had given an inhibition value.
铜纳米颗粒作为一种环境友好型材料的潜在特性,使其在抗菌清洗剂领域的探索需要防止细菌、病毒等微生物的感染或污染。以0.02 M Cu(NO3)2为前驱体,红火龙果提取物为生物还原剂,合成了纳米铜。以甲醇为溶剂,采用浸渍法提取红火果。采用绿色化学还原法制备了铜纳米颗粒。红火果提取物中的化合物将Cu2+还原为Cu。采用红火果萃取液与Cu(NO3)2溶液混合,并以Cu2+前驱体Cu2+体积比为1:1、1:2、1:3、1:4、1:5、1:6、1:7、1:8、1:9的生物还原剂萃取液体积比进行纳米颗粒合成实验。将混合物用磁力搅拌器搅拌30分钟,然后孵育。采用UV-Vis(紫外-可见)、FTIR(傅里叶变换红外)和数码显微镜对样品进行形貌表征。观察继续评估其抗菌活性和效力作为一种有效的消毒剂。紫外-可见分析表明,30min吸光度值趋于稳定。粒径估算表明,当体积比为1:9时,最小粒径为122.12 nm。FTIR官能团分析显示,火龙果提取物和铜纳米颗粒(CuNPs)之间的波数发生了变化,表明两者之间存在官能团相互作用。数码显微镜形态分析结果表明,果实提取物与Cu(NO3)2的配比为1:9,所制得颗粒呈细粉状。对金黄色葡萄球菌和大肠杆菌的抑菌活性表明,铜纳米颗粒的比例为1:9,具有一定的抑菌作用。
{"title":"Green Synthesis of Copper Nanoparticles Using Red Dragon Fruit (Hylocereus polyrhizus) Extract and Its Antibacterial Activity for Liquid Disinfectant","authors":"Cindy Agriningsih Haruna, Widy Aprillia Malik, Muhammad Yaqub Syamsul Rijal, A. H. Watoni, L. Ramadhan","doi":"10.14710/jksa.25.10.352-361","DOIUrl":"https://doi.org/10.14710/jksa.25.10.352-361","url":null,"abstract":"The effort of copper nanoparticle exploration for antibacterial cleaning agents needs to prevent infection or contamination of microorganisms such as bacteria and viruses due to its potential characteristic as an environmentally benign material. The synthesis of copper nanoparticles using 0.02 M Cu(NO3)2 as a precursor and red dragon fruit extract as a bioreduction agent has been conducted. The extraction process of red dragon fruit was done by the maceration method using methanol as solvent. Copper nanoparticles were prepared through green chemistry with the reduction method. The compound in red dragon fruit extract reduced Cu2+ to Cu. The experimental nanoparticle synthesis method was performed with a combination of red dragon fruit extract and Cu(NO3)2 solution mixed with a volume ratio of bioreductor extract toward Cu2+ precursor variation of 1:1, 1:2, 1:3, 1:4, 1:5, 1:6, 1:7, 1:8, and 1:9. The mixtures were stirred using a magnetic stirrer for 30 minutes and then incubated. The samples were characterized using UV-Vis (Ultraviolet-Visible), FTIR (Fourier Transform Infrared), and a digital microscope to observe morphology. The observation continued to assess its antibacterial activity and potency as an active disinfectant. UV-Vis analysis showed that the absorbance value for 30 minutes tended to be stable. Particle size estimation showed the smallest size of 122.12 nm at a volume ratio of 1:9. Functional group analysis using FTIR showed a shift in wavenumber between dragon fruit extract and copper nanoparticle (CuNPs), indicating a functional group interaction. The results of morphological analysis using a digital microscope showed that the particles with fine powder granules were produced with the composition of fruit extract: Cu(NO3)2 of 1:9. Antibacterial activity against Staphylococcus aureus and Escherichia coli indicated that the composition of Cu-nanoparticles of 1:9 had given an inhibition value.","PeriodicalId":17811,"journal":{"name":"Jurnal Kimia Sains dan Aplikasi","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67000137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
期刊
Jurnal Kimia Sains dan Aplikasi
全部 Acc. Chem. Res. ACS Applied Bio Materials ACS Appl. Electron. Mater. ACS Appl. Energy Mater. ACS Appl. Mater. Interfaces ACS Appl. Nano Mater. ACS Appl. Polym. Mater. ACS BIOMATER-SCI ENG ACS Catal. ACS Cent. Sci. ACS Chem. Biol. ACS Chemical Health & Safety ACS Chem. Neurosci. ACS Comb. Sci. ACS Earth Space Chem. ACS Energy Lett. ACS Infect. Dis. ACS Macro Lett. ACS Mater. Lett. ACS Med. Chem. Lett. ACS Nano ACS Omega ACS Photonics ACS Sens. ACS Sustainable Chem. Eng. ACS Synth. Biol. Anal. Chem. BIOCHEMISTRY-US Bioconjugate Chem. BIOMACROMOLECULES Chem. Res. Toxicol. Chem. Rev. Chem. Mater. CRYST GROWTH DES ENERG FUEL Environ. Sci. Technol. Environ. Sci. Technol. Lett. Eur. J. Inorg. Chem. IND ENG CHEM RES Inorg. Chem. J. Agric. Food. Chem. J. Chem. Eng. Data J. Chem. Educ. J. Chem. Inf. Model. J. Chem. Theory Comput. J. Med. Chem. J. Nat. Prod. J PROTEOME RES J. Am. Chem. Soc. LANGMUIR MACROMOLECULES Mol. Pharmaceutics Nano Lett. Org. Lett. ORG PROCESS RES DEV ORGANOMETALLICS J. Org. Chem. J. Phys. Chem. J. Phys. Chem. A J. Phys. Chem. B J. Phys. Chem. C J. Phys. Chem. Lett. Analyst Anal. Methods Biomater. Sci. Catal. Sci. Technol. Chem. Commun. Chem. Soc. Rev. CHEM EDUC RES PRACT CRYSTENGCOMM Dalton Trans. Energy Environ. Sci. ENVIRON SCI-NANO ENVIRON SCI-PROC IMP ENVIRON SCI-WAT RES Faraday Discuss. Food Funct. Green Chem. Inorg. Chem. Front. Integr. Biol. J. Anal. At. Spectrom. J. Mater. Chem. A J. Mater. Chem. B J. Mater. Chem. C Lab Chip Mater. Chem. Front. Mater. Horiz. MEDCHEMCOMM Metallomics Mol. Biosyst. Mol. Syst. Des. Eng. Nanoscale Nanoscale Horiz. Nat. Prod. Rep. New J. Chem. Org. Biomol. Chem. Org. Chem. Front. PHOTOCH PHOTOBIO SCI PCCP Polym. Chem.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1