Depression is a serious disorder with a large impact on both an individual's quality of life and society as a whole. This study aimed to evaluate the potential involvement of nuclear factor kappa-B (NF-kB) and the Janus activated kinase (JAK) and Signal transducer and activator of transcription proteins (STAT) signaling pathway in the pathogenesis of genetically predisposed depression in female rats. The obtained results showed increased phosphorylation in JAK2 and STAT3, and increased protein levels of NF-kB in the hippocampus of Wistar Kyoto rats compared to Wistar rats. These results suggest that disturbance in these pathways could have a significant role in the pathophysiology of genetically predisposed depression in females.
抑郁症是一种严重的疾病，对个人的生活质量和整个社会都有很大的影响。本研究旨在探讨核因子κ b (NF-kB)、Janus活化激酶(JAK)和转录蛋白信号转导和激活因子(STAT)信号通路在雌性大鼠遗传易感性抑郁症发病中的潜在作用。结果显示，与Wistar大鼠相比，Wistar Kyoto大鼠海马中JAK2和STAT3磷酸化水平升高，NF-kB蛋白水平升高。这些结果表明，这些通路的紊乱可能在女性遗传易感性抑郁症的病理生理中起重要作用。
{"title":"Dysregulation of NF-kB and JAK2/STAT3 signaling in the hippocampus of female WKY strain, a genetic animal model of depression","authors":"Bojana Stefanović, Kristina Virijević, Nataša Spasojević, Harisa Ferizović, Milica Janković, Perica Vasljević, Slađana Dronjak","doi":"10.5937/kgjsci2345149s","DOIUrl":"https://doi.org/10.5937/kgjsci2345149s","url":null,"abstract":"Depression is a serious disorder with a large impact on both an individual's quality of life and society as a whole. This study aimed to evaluate the potential involvement of nuclear factor kappa-B (NF-kB) and the Janus activated kinase (JAK) and Signal transducer and activator of transcription proteins (STAT) signaling pathway in the pathogenesis of genetically predisposed depression in female rats. The obtained results showed increased phosphorylation in JAK2 and STAT3, and increased protein levels of NF-kB in the hippocampus of Wistar Kyoto rats compared to Wistar rats. These results suggest that disturbance in these pathways could have a significant role in the pathophysiology of genetically predisposed depression in females.","PeriodicalId":17829,"journal":{"name":"Kragujevac Journal of Science","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136138658","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Aca Đurđević, Miloš Popović, Ivan Medenica, Marko Nikolić
During a field survey in 2021 in Southern Serbia, near the village of Strezovac, two specimens of Cordulegaster insignis Schneider, 1845 were found. This species is extremely rare in Serbia. The findings are located on the western border of its areal.
{"title":"New finding of Cordulegaster insignis Schneider, 1845 (odonata: cordulegastridae) in Serbia","authors":"Aca Đurđević, Miloš Popović, Ivan Medenica, Marko Nikolić","doi":"10.5937/kgjsci2345335d","DOIUrl":"https://doi.org/10.5937/kgjsci2345335d","url":null,"abstract":"During a field survey in 2021 in Southern Serbia, near the village of Strezovac, two specimens of Cordulegaster insignis Schneider, 1845 were found. This species is extremely rare in Serbia. The findings are located on the western border of its areal.","PeriodicalId":17829,"journal":{"name":"Kragujevac Journal of Science","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136138660","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Željko Cimbaljević, Milena Živković, Marko Milošević, Dragana Krstić, Tatjana Miladinović, Vladimir Marković, Nenad Stevanović
Examination of radioactivity in edible liquids is very important because large amounts are taken into the body during the day. This scientific paper presents a comprehensive study on the gamma spectrometry analysis and health risk assessment of edible liquids. The study involved samples of various liquid products such as alcoholic beverages, juices, and milk. Milk is an important part of our regular diet for everyone, from newborns to adults, so we determined the active concentrations of radionuclides for both milk from farms directly and milk bought in a supermarket. The gamma spectrometry analysis was performed using a high-purity germanium detector to determine the concentration of natural and artificial radionuclides. The radioactivity of each sample is presented as the mean value of the measured sample from five different manufacturers. In conclusion, the gamma spectrometry analysis and health risk assessment of edible liquids demonstrated that the concentration of radionuclides in the selected liquid food products was not a major health concern. However, continuous monitoring and evaluation are recommended to ensure that the safety of the products is maintained within the permissible limits.
{"title":"Gamma spectrometry analysis and health risk assessment of edible liquids","authors":"Željko Cimbaljević, Milena Živković, Marko Milošević, Dragana Krstić, Tatjana Miladinović, Vladimir Marković, Nenad Stevanović","doi":"10.5937/kgjsci2345015c","DOIUrl":"https://doi.org/10.5937/kgjsci2345015c","url":null,"abstract":"Examination of radioactivity in edible liquids is very important because large amounts are taken into the body during the day. This scientific paper presents a comprehensive study on the gamma spectrometry analysis and health risk assessment of edible liquids. The study involved samples of various liquid products such as alcoholic beverages, juices, and milk. Milk is an important part of our regular diet for everyone, from newborns to adults, so we determined the active concentrations of radionuclides for both milk from farms directly and milk bought in a supermarket. The gamma spectrometry analysis was performed using a high-purity germanium detector to determine the concentration of natural and artificial radionuclides. The radioactivity of each sample is presented as the mean value of the measured sample from five different manufacturers. In conclusion, the gamma spectrometry analysis and health risk assessment of edible liquids demonstrated that the concentration of radionuclides in the selected liquid food products was not a major health concern. However, continuous monitoring and evaluation are recommended to ensure that the safety of the products is maintained within the permissible limits.","PeriodicalId":17829,"journal":{"name":"Kragujevac Journal of Science","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136138956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Anđela Perić, Ana Mirić, Jelena Košarić, Nevena Milivojević, Marko Živanović, Neda Ćićarić, Petar Arsenijević
There is a wide group of ovarian tumors, malignant as well as benign. Histopathological examination is used as a primary source of identifying the difference between malignant and benign processes. We analyzed ovarian tissue samples of twelve women, ages ranging from 21 to 77. Samples of 8 benign, 1 borderline, and 3 malignant ovarian tumors were included in the study. Using the quantitative PCR method, genes indicating apoptotic processes in tissue cells were evaluated, based on monitoring their relative expression. Relative gene expression was monitored for anti-apoptotic protein Bcl-2, pro-apoptotic protein Bax, and effector protein Cas3. Expression of gene for protein Bax and Bcl-2 is increased and Bax predominates, which indicates that the initiation of apoptosis has begun, mostly in benign tumors. Bcl-2 was elevated in samples of borderline and malignant tumors while Bax was decreased, which indicates the inhibition of apoptosis in these samples.
{"title":"Expression of proapoptotic-antiapoptotic genes in malignant, borderline and benign ovarian tumors","authors":"Anđela Perić, Ana Mirić, Jelena Košarić, Nevena Milivojević, Marko Živanović, Neda Ćićarić, Petar Arsenijević","doi":"10.5937/kgjsci2345121p","DOIUrl":"https://doi.org/10.5937/kgjsci2345121p","url":null,"abstract":"There is a wide group of ovarian tumors, malignant as well as benign. Histopathological examination is used as a primary source of identifying the difference between malignant and benign processes. We analyzed ovarian tissue samples of twelve women, ages ranging from 21 to 77. Samples of 8 benign, 1 borderline, and 3 malignant ovarian tumors were included in the study. Using the quantitative PCR method, genes indicating apoptotic processes in tissue cells were evaluated, based on monitoring their relative expression. Relative gene expression was monitored for anti-apoptotic protein Bcl-2, pro-apoptotic protein Bax, and effector protein Cas3. Expression of gene for protein Bax and Bcl-2 is increased and Bax predominates, which indicates that the initiation of apoptosis has begun, mostly in benign tumors. Bcl-2 was elevated in samples of borderline and malignant tumors while Bax was decreased, which indicates the inhibition of apoptosis in these samples.","PeriodicalId":17829,"journal":{"name":"Kragujevac Journal of Science","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136138964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jelena Pavić, Marko Živanović, Katarina Virijević, Irena Tanasković, Vesna Stanković, Nebojša Marić, Danijela Cvetković, Nenad Filipović
Colorectal cancer is one of the leading causes of mortality worldwide. It is the most common malignancy and there is a need for new approaches in therapies. Surgery, radiation and chemotherapy are the key components of colon cancer treatment. Besides common chemotherapy, alternative therapies are being studied to increase treatment effectiveness and reduce side effects. In this article, colorectal carcinoma cells were treated with chemotherapeutics and relative gene expression was investigated for the genes coding cytoskeleton proteins: CDH1, CTNNB1 and CDH2; for redox status genes: GPX1, GPX2, GPX3, GPX4, TXNRD1, GSTP1, NFE2L2, NFKB1, HIF1A; and for apoptosis genes: CASP3, CASP8, CASP9, FAS, BCL-2 and BAX. The results of our research showed that some concentrations of chemotherapeutics increased the expression of certain genes. Molecular alterations that lead to colorectal cancer can determine appropriate and effective treatment - chemotherapeutics, as well as the design of direct therapeutic targets.
{"title":"Influence of cytostatics on relative gene expression in redox status, apoptosis and migration colorectal carcinoma model system","authors":"Jelena Pavić, Marko Živanović, Katarina Virijević, Irena Tanasković, Vesna Stanković, Nebojša Marić, Danijela Cvetković, Nenad Filipović","doi":"10.5937/kgjsci2345159p","DOIUrl":"https://doi.org/10.5937/kgjsci2345159p","url":null,"abstract":"Colorectal cancer is one of the leading causes of mortality worldwide. It is the most common malignancy and there is a need for new approaches in therapies. Surgery, radiation and chemotherapy are the key components of colon cancer treatment. Besides common chemotherapy, alternative therapies are being studied to increase treatment effectiveness and reduce side effects. In this article, colorectal carcinoma cells were treated with chemotherapeutics and relative gene expression was investigated for the genes coding cytoskeleton proteins: CDH1, CTNNB1 and CDH2; for redox status genes: GPX1, GPX2, GPX3, GPX4, TXNRD1, GSTP1, NFE2L2, NFKB1, HIF1A; and for apoptosis genes: CASP3, CASP8, CASP9, FAS, BCL-2 and BAX. The results of our research showed that some concentrations of chemotherapeutics increased the expression of certain genes. Molecular alterations that lead to colorectal cancer can determine appropriate and effective treatment - chemotherapeutics, as well as the design of direct therapeutic targets.","PeriodicalId":17829,"journal":{"name":"Kragujevac Journal of Science","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136138661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Marko Milošević, Željko Cimbaljević, Milena Živković, Nenad Stevanović, Vladimir Marković, Dragana Krstić
As mobile phones have become a necessity in modern society, and there is an increasing number of users of various ages, it is imperative to investigate the impact of mobile phone radiofrequency electromagnetic radiation (RF) on humans. Because the human head is one of the most exposed and sensitive organs in the body, this paper investigated the propagation of the electromagnetic (EM) field through it. Additionally, the Specific absorption rate (SAR) was examined to study how RF radiation affects the human head. A program code using the finite-difference time-domain (FDTD) method was created to simulate the propagation of RF radiation through the six layers that make up the human head (skin, fat, bone, dura mater, CSF, and brain). RF radiation at the following frequencies was investigated: 0.9 GHz, 1.8 GHz, 2.4 GHz, 3.35 GHz, and 4.5 GHz. Values of the thickness and parameters of the layers can also be changed in the program. It was discovered that the majority of the EM energy is absorbed in the skin of the human head.
{"title":"Analysis of Specific absorption rate in the human head model exposed to radiofrequency radiation","authors":"Marko Milošević, Željko Cimbaljević, Milena Živković, Nenad Stevanović, Vladimir Marković, Dragana Krstić","doi":"10.5937/kgjsci2345007m","DOIUrl":"https://doi.org/10.5937/kgjsci2345007m","url":null,"abstract":"As mobile phones have become a necessity in modern society, and there is an increasing number of users of various ages, it is imperative to investigate the impact of mobile phone radiofrequency electromagnetic radiation (RF) on humans. Because the human head is one of the most exposed and sensitive organs in the body, this paper investigated the propagation of the electromagnetic (EM) field through it. Additionally, the Specific absorption rate (SAR) was examined to study how RF radiation affects the human head. A program code using the finite-difference time-domain (FDTD) method was created to simulate the propagation of RF radiation through the six layers that make up the human head (skin, fat, bone, dura mater, CSF, and brain). RF radiation at the following frequencies was investigated: 0.9 GHz, 1.8 GHz, 2.4 GHz, 3.35 GHz, and 4.5 GHz. Values of the thickness and parameters of the layers can also be changed in the program. It was discovered that the majority of the EM energy is absorbed in the skin of the human head.","PeriodicalId":17829,"journal":{"name":"Kragujevac Journal of Science","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136138957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Suzana Leković, Stefan Marković, Đorđe Minić, Jovana Todosijević, Aleksandra Torbica, Nevena Đukić
Barley is an important source of b-glucans because they have a positive influence on human health and potential application in the food industry. The aim of this manuscript was to determine the variability of ten barley cultivars based on b-glucan content. The percentage of b-glucan varied in the range of 4.44% to 8.17%, with a mean value of 6.57%. Based on b-glucan content, the degree of cultivar similarity was determined by the dendrogram. Among analyzed barley cultivars, the highest concentration of b-glucan was found in the cultivar Lav and Ukras, while the lowest concentration was observed in the cultivar Vihor (4.44%). Compared with all the other cultivars mentioned, a pair of cultivars expressed the greatest distance. Statistical analysis showed that there is a significant difference between analyzed barley cultivars. The analysis of b-glucans in varieties of barley in two growing seasons aims to develop an agricultural strategy and develop barley varieties with higher b-glucans content.
{"title":"B-glucan content variability in seed of barley cultivars","authors":"Suzana Leković, Stefan Marković, Đorđe Minić, Jovana Todosijević, Aleksandra Torbica, Nevena Đukić","doi":"10.5937/kgjsci2345111l","DOIUrl":"https://doi.org/10.5937/kgjsci2345111l","url":null,"abstract":"Barley is an important source of b-glucans because they have a positive influence on human health and potential application in the food industry. The aim of this manuscript was to determine the variability of ten barley cultivars based on b-glucan content. The percentage of b-glucan varied in the range of 4.44% to 8.17%, with a mean value of 6.57%. Based on b-glucan content, the degree of cultivar similarity was determined by the dendrogram. Among analyzed barley cultivars, the highest concentration of b-glucan was found in the cultivar Lav and Ukras, while the lowest concentration was observed in the cultivar Vihor (4.44%). Compared with all the other cultivars mentioned, a pair of cultivars expressed the greatest distance. Statistical analysis showed that there is a significant difference between analyzed barley cultivars. The analysis of b-glucans in varieties of barley in two growing seasons aims to develop an agricultural strategy and develop barley varieties with higher b-glucans content.","PeriodicalId":17829,"journal":{"name":"Kragujevac Journal of Science","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136138959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Đukić, R. Mirić, Josip Skejo, Saša Rajkov, Ivana Tot
In this paper we present data on damselflies and dragonflies (Insecta: Odonata) of the Landscape of outstanding features (LOF) “Vlasina”. Most of the data were collected during research camps of the Scientic Research Society of Biology and Ecology Students "Josif Pančić" that took place from 2013 to 2018, when 27 Odonata species were recorded. If this research is combined with previously published data, 37 species of damselflies and dragonflies were recorded in Landscape of Outstanding Features “Vlasina”. The paper presents distribution and reproduction status of the recorded species, as well as their diversity in this area. The most important species is Epitheca bimaculata (Charpentier, 1825), because Vlasina represents one of its southernmost and highest habitats in Europe. For Sympetrum flaveolum (Linnaeus, 1758) Vlasina is one of the most important habitats in Serbia, as well as for Leucorrhinia pectoralis (Charpentier, 1825) which is Natura 2000 species.
{"title":"Survey on the damselfly and dragonfly fauna (Insecta: Odonata) of the Landscape of outstanding features \"Vlasina\"","authors":"A. Đukić, R. Mirić, Josip Skejo, Saša Rajkov, Ivana Tot","doi":"10.5937/KGJSCI1941133D","DOIUrl":"https://doi.org/10.5937/KGJSCI1941133D","url":null,"abstract":"In this paper we present data on damselflies and dragonflies (Insecta: Odonata) of the Landscape of outstanding features (LOF) “Vlasina”. Most of the data were collected during research camps of the Scientic Research Society of Biology and Ecology Students \"Josif Pančić\" that took place from 2013 to 2018, when 27 Odonata species were recorded. If this research is combined with previously published data, 37 species of damselflies and dragonflies were recorded in Landscape of Outstanding Features “Vlasina”. The paper presents distribution and reproduction status of the recorded species, as well as their diversity in this area. The most important species is Epitheca bimaculata (Charpentier, 1825), because Vlasina represents one of its southernmost and highest habitats in Europe. For Sympetrum flaveolum (Linnaeus, 1758) Vlasina is one of the most important habitats in Serbia, as well as for Leucorrhinia pectoralis (Charpentier, 1825) which is Natura 2000 species.","PeriodicalId":17829,"journal":{"name":"Kragujevac Journal of Science","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48276675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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