Pub Date : 2020-01-01DOI: 10.22889/KJP.2020.51.3.192
kimjunhyeong, Kang Mu Kwon, E. Lee, Daekeun Kim, Jeong-Sook Park, Jae hyeok Lee
Caenorhabditis elegans model system was used to investigate the antioxidant activity of Beta vulgaris L. (Chenopodiaceae) methanol extract. The methanol extract showed DPPH radical scavenging and superoxide quenching activity in a dose-dependent manner. The B. vulgaris methanol extract was measured for the activity of superoxide dismutase (SOD), catalase, and oxidative stress tolerance by using C. elegans, along with reactive oxygen species (ROS) level. In addition, to see that the regulation of the stress response gene is responsible for the increased stress tolerance of C. elegans treated by the methanol extract, SOD-3 expression was measured using a transgenic strain. As a result, the B. vulgaris methanol extract increased SOD and catalase activities, and decreased ROS accumulation, dose-dependently. Furthermore, the methanol extract-treated CF1553 worm showed higher SOD-3::GFP intensity than the control.
采用秀丽隐杆线虫(Caenorhabditis elegans)模型系统研究了Beta vulgaris L. (Chenopodiaceae)甲醇提取物的抗氧化活性。甲醇提取物对DPPH自由基的清除和超氧化物的猝灭活性呈剂量依赖性。测定了芥蓝甲醇提取物的超氧化物歧化酶(SOD)、过氧化氢酶(过氧化氢酶)活性和秀丽隐杆线虫的氧化应激耐受性，以及活性氧(ROS)水平。此外，为了了解应激反应基因的调控是否与秀丽隐杆线虫经甲醇提取物处理后的抗逆性增加有关，我们利用转基因菌株检测了SOD-3的表达。结果表明，野刺草甲醇提取物增加了SOD和过氧化氢酶活性，降低了ROS积累，且呈剂量依赖性。此外，甲醇提取物处理的CF1553蠕虫的SOD-3::GFP强度高于对照。
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Pub Date : 2020-01-01DOI: 10.22889/KJP.2020.51.4.297
Juree Kim, L. Paje, Jungwon Choi, Hak-Dong Lee, J. Shim, J. Shim, P. J. Geraldino, Sanghyun Lee
{"title":"Determination of Silymarin and Silybin Diastereomers in Korean Milk Thistle using HPLC/UV Analysis","authors":"Juree Kim, L. Paje, Jungwon Choi, Hak-Dong Lee, J. Shim, J. Shim, P. J. Geraldino, Sanghyun Lee","doi":"10.22889/KJP.2020.51.4.297","DOIUrl":"https://doi.org/10.22889/KJP.2020.51.4.297","url":null,"abstract":"","PeriodicalId":17851,"journal":{"name":"Korean Journal of Pharmacognosy","volume":"96 1","pages":"297-301"},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82777142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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