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Effects of Sasa borealis silage on proximate composition, amino acid and fatty acid contents, and antioxidant activity in fresh meat of Korean native goat fed with total mixed ration 北方莎草青贮对全混合日粮饲喂朝鲜土山羊鲜肉近似组成、氨基酸和脂肪酸含量及抗氧化活性的影响
Q4 Agricultural and Biological Sciences Pub Date : 2023-02-01 DOI: 10.11002/kjfp.2023.30.1.15
Young-Jin Choi, S. Chung, Na Yeon Kim, M. Oh, S. Jang, Young-Sik Yun, S. Moon
Jeju Sasa borealis (S. borealis) is indigenous to the Halla Mountain area of Jeju Island, Republic of Korea. However, its dominance has retarded the development of other plant species and lowered biodiversity in this region. The aim of this study was to determine whether S. borealis silage (SS) supplementation affects the chemical composition and antioxidant activity in the fresh meat of Korean native goats (Capra hircus coreanae). The experiment was conducted on 12 Korean native goats at the finisher stage. The feeding groups were the Control (total mixed ration, TMR) and the Treatment (80% TMR + 20% SS). The animals were adapted for two weeks and then subjected to a six-month breeding experiment. Meat samples were excised from the neck, loin, rib, front leg, and hind leg of the slaughtered animals. The meat derived from the treatment group contained more taurine and anserine than that derived from the control group. Both groups did not significantly differ in terms of ω-6/ω-3 fatty acid ratio. The loin and front leg of the treatment group contained significantly higher vitamin E levels than those of the control group. DPPH, ABTS, and FRAP analyses disclosed that the loin and front leg had significantly higher antioxidant activity (p⟨0.05) than the other parts. Moreover, the loin and front leg cuts of the treatment group had higher antioxidant activity than those of the control group. The present study demonstrated that S. borealis supplementation could effectively improve Korean native goat meat quality.
济州北方沙草(S. borealis)是韩国济州岛汉拿山地区的土著植物。然而,其优势地位阻碍了其他植物物种的发展,降低了该地区的生物多样性。本研究的目的是研究添加北方青贮菌(S. borealis青贮,SS)是否会影响韩国本地山羊(Capra hircus coreanae)鲜肉的化学成分和抗氧化活性。本试验以12只育肥期韩国本地山羊为试验对象。饲喂组为对照组(全混合日粮,TMR)和试验组(80% TMR + 20% SS)。这些动物先适应两周,然后进行为期六个月的繁殖实验。从被屠宰动物的颈部、腰部、肋骨、前腿和后腿上切除肉样本。处理组的肉比对照组的肉含有更多的牛磺酸和雁胺。两组ω-6/ω-3脂肪酸比值无显著差异。治疗组腰部和前腿的维生素E含量明显高于对照组。DPPH、ABTS和FRAP分析显示,腰和前腿的抗氧化活性显著高于其他部位(p⟨0.05)。治疗组腰、前腿部位的抗氧化活性明显高于对照组。本研究表明,在山羊肉中添加北方葡萄球菌可以有效地改善韩国土产山羊肉的品质。
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引用次数: 0
Vitamin D analysis in the Korean total diet study and UV/sun light irradiated mushrooms 韩国总膳食研究中的维生素D分析和紫外线/阳光照射蘑菇
Q4 Agricultural and Biological Sciences Pub Date : 2023-02-01 DOI: 10.11002/kjfp.2023.30.1.109
M. Seo, I. Roh, J. Lee, Sung-Ok Kwon, Chorong Kim, G. Lee
This study was conducted to evaluate vitamin D intake of Koreans in a total diet study (TDS) and to determine the effect of irradiation on vitamin D synthesis in mushrooms. For analysis, sample were saponified and extracted with hexane, and vitamin D was quantified by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Based on the validation results, the recovery of the National Institute of Standards and Technology (NIST) standard reference sample (SRM) 1849a was 96.7% and the z-score of −1.6 was obtained by the Food Analysis Performance Assessment Scheme (FAPAS) proficiency test (PT) 21115. Vitamin D2 was not detected in any samples, and the highest level of vitamin D3 was detected in mackerel and anchovies ranging from 24.2 to 120.2 μg/kg. The mean daily intake of vitamin D was 0.99 μg/day, as estimated from the vitamin D contents of the analyzed foods and their corresponding intake. The adequate intake (AI) of vitamin D based on the Dietary reference intakes for Koreans provided by the Ministry of Health and Welfare is 5-15 μg/day for Koreans aged 6 to 75 years. Compared with this AI, vitamin D intake of Koreans estimated in this study was inadequate. For that, the increased vitamin D content in ultraviolet (UV)/sun light irradiated mushrooms warrants further research to increase vitamin D intake of Koreans through diet.
本研究旨在评估韩国人在总饮食研究(TDS)中的维生素D摄入量,并确定辐照对蘑菇中维生素D合成的影响。样品经己烷皂化提取,采用液相色谱-串联质谱法(LC-MS/MS)测定维生素D含量。验证结果表明,美国国家标准技术研究院(NIST)标准参比样品(SRM) 1849a的回收率为96.7%,食品分析性能评估方案(FAPAS)熟练度测试(PT) 21115的z-score为−1.6。所有样品均未检测到维生素D2,鲭鱼和凤尾鱼中维生素D3含量最高,为24.2 ~ 120.2 μg/kg。根据所分析食品中维生素D的含量及其相应的摄入量估算,维生素D的平均日摄入量为0.99 μg/ D。根据保健福利部提供的“国民膳食参考摄取量”,6 ~ 75岁的国民每天维生素D的适当摄取量为5 ~ 15 μg。与此相比,本研究估计的韩国人维生素D摄入量不足。因此,在紫外线照射下的蘑菇中维生素D含量的增加值得进一步研究,以通过饮食增加韩国人的维生素D摄入量。
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引用次数: 0
Influence of low-pressure tumbling on the quality characteristics of thawed pork 低压翻滚对解冻猪肉品质特性的影响
Q4 Agricultural and Biological Sciences Pub Date : 2023-02-01 DOI: 10.11002/kjfp.2023.30.1.88
Won-Ho Hong, Jeong-Sang Kim, Yu-Jeong Gwak, Jiyeon Chun
As livestock consumption in Korea has been gradually increasing, the quality of the final products has been improved to meet this increased demand. In particular, maintaining the water holding capacity (WHC) and minimizing the drip loss during the thawing of frozen meat are of utmost importance. This study investigated the physicochemical properties of frozen pork subjected to thawing under different conditions: at room temperature (20°C, under air), at a low temperature (4°C refrigerator, under air), under water (20°C, under water in a vacuum bag), under microwave (microwave-thawing, 260 W), and under low-pressure tumbling (20°C, 0.015 bar, tumbling). The shortest thawing time for frozen pork was recorded upon low-pressure tumbling thus indicating a fast heat transfer. The lowest drip loss (0.2%) and highest WHC (94.5%) were also recorded under this condition. A significantly higher drip loss was observed upon microwave-(1.0%) and water-thawing (1.2%), which resulted in the lowest WHC in microwave thawing (87.2%). The highest total count of aerobic bacteria and coliform group were observed upon room temp thawing while the low pressure tumbling and thawing resulted in the lowest aerobic bacteria (1.90 log CFU/g) and coliform (0.78 log CFU/g) count. Consequently, thawing by low pressure tumbling afforded the best food quality.
随着韩国牲畜消费量的逐渐增加,最终产品的质量也得到了提高,以满足日益增长的需求。特别是,在冷冻肉解冻过程中,保持保水能力和最大限度地减少滴水损失至关重要。本研究研究了在不同条件下解冻的冷冻猪肉的理化性质:室温(20°C,空气下)、低温(4°C冰箱,空气中)、水下(20°C.真空袋中的水下)、微波(微波解冻,260W)和低压翻滚(20°C.0.015bar,翻滚)。冷冻猪肉的解冻时间最短是在低压翻滚时记录的,因此表明热传递很快。在这种条件下也记录到最低的滴水损失(0.2%)和最高的WHC(94.5%)。在微波(1.0%)和水解冻(1.2%)时观察到显著更高的滴水损失,这导致微波解冻中的WHC最低(87.2%)。在室温解冻时观察到最高的需氧菌总数和大肠菌群,而低压翻滚和解冻导致最低的需氧菌数(1.90 log CFU/g)和大肠菌数(0.78 log CFU/g)。因此,通过低压翻滚解冻提供了最佳的食品质量。
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引用次数: 0
Comparative analysis of nutritional components in various parts of Hovenia dulcis Thunbergii 枳壳不同部位营养成分的比较分析
Q4 Agricultural and Biological Sciences Pub Date : 2023-02-01 DOI: 10.11002/kjfp.2023.30.1.1
Su-Hwan Kim, Jung-Won Lee, C. Huh
In this study, the nutritional compounds present in various parts of Hovenia dulcis Thunbergii (H. dulcis) were compared. Regarding the free sugar content, fruits exhibited the highest fructose concentration (9.42 g/100 g), whereas branches (2.46 g/100 g) and leaves (5.82 g/100 g) contained the highest glucose levels. The most common types of organic acids were citric and tartaric acids in the fruits, citric and malic acids in the branches, and malic and succinic acids in the leaves. The leaves exhibited the highest total amino acid content of 12,102.91 mg/100 g, whereas vitamin C is predominantly found in branches and leaves at 367.85 mg/100 g and 336.21 mg/100 g, respectively. In H. dulcis, β-carotene was present in high concentration in leaves (2.41 mg/100 g), whereas the branches (0.15 mg/100 g) and fruits (0.09 mg/100 g) contain smaller amounts of it. Vitamin E, α-tocopherol, was present in high concentrations in the fruit (11.01 mg/100 g), branches (6.61 mg/100 g), and leaves (11.01 mg/100 g).
本研究对枳壳不同部位的营养成分进行了比较。关于游离糖含量,水果的果糖浓度最高(9.42 g/100 g),而枝条(2.46 g/100 g和叶片(5.82 g/100克)的葡萄糖水平最高。最常见的有机酸类型是水果中的柠檬酸和酒石酸,树枝中的柠檬酸和苹果酸,以及叶子中的苹果酸和琥珀酸。叶片的总氨基酸含量最高,为12102.91 mg/100g,而维生素C主要存在于枝条和叶片中,分别为367.85 mg/100g和336.21 mg/100g。在杜尔cis中,β-胡萝卜素在叶片中的浓度很高(2.41 mg/100 g),而枝条(0.15 mg/100 g)和果实(0.09 mg/100克)中的含量较少。维生素E,α-生育酚,在果实(11.01 mg/100克)、枝条(6.61 mg/100 g)和叶片(11.01 g/100 g)中的浓度较高。
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引用次数: 0
Physicochemical characteristics of carotenoid-enriched extract prepared from persimmon peels by ultrasound-assisted extraction with soybean oil solvent 超声辅助大豆油萃取柿皮富含类胡萝卜素提取物的理化特性研究
Q4 Agricultural and Biological Sciences Pub Date : 2023-02-01 DOI: 10.11002/kjfp.2023.30.1.122
Hun-Sik Chung, K. Youn, Soo Won Lee, Hey-Kyung Moon, Jong Kuk Kim
Persimmon peels are mostly discared as a by-product of dried persimmon manufacturing, but effective use is required as it contains various functional constituents. This study covers the preparation of carotenoid-enriched oil by ultrasound treatment of persimmon peel in soybean oil solvent, and the investigation of its physicochemical characteristics. Using the Box-Behnken design and response surface methodology, the optimal extraction conditions (temperature, 62°C; time, 32 min; and persimmon peel to oil ratio, 1:3.1) were determined based on the carotenoid concentration of the extract. The physicochemical characteristics of the extract obtained under optimal conditions and the untreated soybean oil (control) were compared. The total carotenoid content of the extract increased. The carotenoid-enriched soybean oils had a lighter color than the controls, but with high redness and yellowness values. The effect of sonication and a component of the persimmon peel on the oxidation and heating stability of soybean oil was weak. The viscosity and activation energy of carotenoid-enriched soybean oil were slightly higher than those of the control. Thus, it was possible to prepare yellow-red carotenoid-enriched soybean oil by applying ultrasonic-soybean oil solvent extraction to persimmon peel. The oil is expected to be useful as an additive as well as a substitute for general edible oils.
柿子皮大多被认为是干柿子生产的副产品,但由于其含有多种功能成分,因此需要有效使用。本研究采用大豆油溶剂对柿子皮进行超声处理,制备富含类胡萝卜素的油脂,并对其理化性质进行了研究。使用Box-Behnken设计和响应面方法,基于提取物中的类胡萝卜素浓度确定了最佳提取条件(温度,62°C;时间,32分钟;柿子皮油比,1:3.1)。比较了在最佳条件下获得的提取物和未处理的大豆油(对照)的理化特性。提取物中的总类胡萝卜素含量增加。富含类胡萝卜素的大豆油的颜色比对照品浅,但具有高的红度和黄度值。超声波处理和柿子皮中的一种成分对大豆油的氧化和加热稳定性的影响较弱。富含类胡萝卜素的大豆油的粘度和活化能略高于对照。因此,应用超声波大豆油溶剂萃取柿子皮,可以制备富含黄红色类胡萝卜素的大豆油。该油有望作为添加剂以及普通食用油的替代品使用。
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引用次数: 0
Production of highly enriched GABA through Lactobacillus plantarum fermentation of katsuobushi protein hydrolyzate made from Dendropanax morbiferus extract fermented by Bacillus subtilis 植物乳杆菌发酵生产高浓度GABA
Q4 Agricultural and Biological Sciences Pub Date : 2023-02-01 DOI: 10.11002/kjfp.2023.30.1.146
Yu-Jeong An, N. Sung, S. Lee
To develop a multi-functional ingredient, the bioconversion of katsuobushi protein was optimized using Bacillus subtilis HA and Lactobacillus plantarum KS2020. The Dendropanax morbiferus extract (DME) culture with protease activity (102 unit/mL) was prepared by B. subtilis with 2% glucose and 1% skim milk through one day of alkaline fermentation. Katsuobushi protein was effectively hydrolyzed by the DME culture at 60°C for 3 hours, resulting in a tyrosine content of 156.85 mg%. Subsequently, a second lactic acid fermentation was carried out with 10% monosodium glutamate (MSG) using L. plantarum KS2020 to produce higher levels of GABA. Following co-cultivation for three days, DME exhibited a pH of 8.3 (0% acidity). After seven days, the viable cell count of L. plantarum increased to 9.33 CFU/mL, but viable Bacillus cells were not detected. Taken together, a multi-functional ingredient with enriched GABA, peptides, probiotics, and umami flavor was developed through lactic acid fermentation using hydrolyzed katsuobushi protein. These results indicate that katsuobushi protein could be used as a byproduct to produce a palatable protein hydrolysate using alkaline-fermented DME culture as a proteolytic enzyme source.
为了开发一种多功能成分,使用枯草芽孢杆菌HA和植物乳杆菌KS2020对katsuobushi蛋白的生物转化进行了优化。以枯草芽孢杆菌为原料,以2%葡萄糖和1%脱脂乳为原料,经过一天的碱性发酵,制备了蛋白酶活性为102单位/mL的毛五加提取物(DME)。DME在60°C下培养3小时,可有效水解Katsuo bushi蛋白,酪氨酸含量为156.85 mg%。随后,使用植物乳杆菌KS2020用10%味精(MSG)进行第二次乳酸发酵,以产生更高水平的GABA。在共培养三天后,DME表现出8.3的pH(0%的酸度)。7天后,植物乳杆菌的活细胞计数增加到9.33 CFU/mL,但未检测到活的芽孢杆菌细胞。总之,利用水解katsuobushi蛋白通过乳酸发酵,开发出了一种富含GABA、肽、益生菌和鲜味的多功能成分。这些结果表明,katsuobushi蛋白可以作为副产品,利用碱性发酵DME培养物作为蛋白水解酶来源生产美味的蛋白质水解物。
{"title":"Production of highly enriched GABA through Lactobacillus\u0000 plantarum fermentation of katsuobushi protein\u0000 hydrolyzate made from Dendropanax morbiferus extract\u0000 fermented by Bacillus subtilis","authors":"Yu-Jeong An, N. Sung, S. Lee","doi":"10.11002/kjfp.2023.30.1.146","DOIUrl":"https://doi.org/10.11002/kjfp.2023.30.1.146","url":null,"abstract":"\u0000 \u0000 To develop a multi-functional ingredient, the bioconversion of\u0000 katsuobushi protein was optimized using Bacillus\u0000 subtilis HA and Lactobacillus plantarum KS2020.\u0000 The Dendropanax morbiferus extract (DME) culture with protease\u0000 activity (102 unit/mL) was prepared by B. subtilis with\u0000 2% glucose and 1% skim milk through one day of alkaline\u0000 fermentation. Katsuobushi protein was effectively hydrolyzed by\u0000 the DME culture at 60°C for 3 hours, resulting in a tyrosine content of\u0000 156.85 mg%. Subsequently, a second lactic acid fermentation was carried\u0000 out with 10% monosodium glutamate (MSG) using L.\u0000 plantarum KS2020 to produce higher levels of GABA. Following\u0000 co-cultivation for three days, DME exhibited a pH of 8.3 (0% acidity).\u0000 After seven days, the viable cell count of L. plantarum\u0000 increased to 9.33 CFU/mL, but viable Bacillus cells were not\u0000 detected. Taken together, a multi-functional ingredient with enriched GABA,\u0000 peptides, probiotics, and umami flavor was developed through lactic acid\u0000 fermentation using hydrolyzed katsuobushi protein. These\u0000 results indicate that katsuobushi protein could be used as a\u0000 byproduct to produce a palatable protein hydrolysate using alkaline-fermented\u0000 DME culture as a proteolytic enzyme source.\u0000","PeriodicalId":17875,"journal":{"name":"Korean Journal of Food Preservation","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49656408","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Determination of vitamin B1 and B3 contents in Korean domestic foods using high performance liquid chromatography 高效液相色谱法测定国产食品中维生素B1和B3的含量
Q4 Agricultural and Biological Sciences Pub Date : 2023-02-01 DOI: 10.11002/kjfp.2023.30.1.98
Song-Yee Lee, So-Ra Choi, E. Song, Eun-Ju Kim, Hyun-Ah Han
The purpose of this study was to determine the vitamin B1 and B3 contents in 135 Korean domestic foods using high performance liquid chromatography (HPLC). The Standard Reference Material (SRM) 3233 and in-house control (grain, whole wheat and flour mixture) were used for quality control/assurance. As a result, vitamin B1 was only detected in 54 of the 135 foods, ranging from 0.019 mg/100 g to 28.218 mg/100 g. Among the foods tested, the vitamin B1 content was highest in ramen powder (28.218 mg/100 g) and lowest in boiled jjolmyeon (0.019 mg/100 g). In contrast, vitamin B3 was detected in 108 of the 135 foods, ranging from 0.077 mg/100 g to 32.412 mg/100 g. We found that the vitamin B3 content was highest in beef liver (32.412 mg/100 g) and lowest in boiled chicken heart (0.077 mg/100 g). The results of this study will be used as a nutritional database for the update of the Korean food composition table and will hence contribute to improving public health.
本研究采用高效液相色谱法(HPLC)测定了135种国产食品中维生素B1和B3的含量。标准参考物质(SRM) 3233和内部控制(谷物,全麦和面粉混合物)用于质量控制/保证。结果,135种食物中只有54种检测到维生素B1,范围从0.019毫克/100克到28.218毫克/100克。在被调查的食品中,维生素B1含量最高的是拉面(28.218毫克/100克),最低的是煮面(0.019毫克/100克),而维生素B3含量在135种食品中有108种(0.077毫克/100克~ 32.412毫克/100克)。研究结果显示,牛肉肝中维生素B3含量最高(32.412 mg/100 g),水煮鸡心中维生素B3含量最低(0.077 mg/100 g)。该研究结果将作为韩国食品成分表更新的营养数据库,从而有助于改善公众健康。
{"title":"Determination of vitamin B1 and B3 contents\u0000 in Korean domestic foods using high performance liquid\u0000 chromatography","authors":"Song-Yee Lee, So-Ra Choi, E. Song, Eun-Ju Kim, Hyun-Ah Han","doi":"10.11002/kjfp.2023.30.1.98","DOIUrl":"https://doi.org/10.11002/kjfp.2023.30.1.98","url":null,"abstract":"\u0000 \u0000 The purpose of this study was to determine the vitamin B1 and\u0000 B3 contents in 135 Korean domestic foods using high performance\u0000 liquid chromatography (HPLC). The Standard Reference Material (SRM) 3233 and\u0000 in-house control (grain, whole wheat and flour mixture) were used for quality\u0000 control/assurance. As a result, vitamin B1 was only detected in 54 of\u0000 the 135 foods, ranging from 0.019 mg/100 g to 28.218 mg/100 g. Among the foods\u0000 tested, the vitamin B1 content was highest in ramen powder (28.218\u0000 mg/100 g) and lowest in boiled jjolmyeon (0.019 mg/100 g). In\u0000 contrast, vitamin B3 was detected in 108 of the 135 foods, ranging\u0000 from 0.077 mg/100 g to 32.412 mg/100 g. We found that the vitamin B3\u0000 content was highest in beef liver (32.412 mg/100 g) and lowest in boiled chicken\u0000 heart (0.077 mg/100 g). The results of this study will be used as a nutritional\u0000 database for the update of the Korean food composition table and will hence\u0000 contribute to improving public health.\u0000","PeriodicalId":17875,"journal":{"name":"Korean Journal of Food Preservation","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41760224","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Immunomodulatory effects of six Acetobacter pasteurianus strains in RAW-Blue macrophage 6株巴氏醋酸杆菌对RAW-Blue巨噬细胞的免疫调节作用
Q4 Agricultural and Biological Sciences Pub Date : 2023-02-01 DOI: 10.11002/kjfp.2023.30.1.65
S. Kim, W. Jeong, So-Young Kim, S. Yeo
In this study, we investigated the immunological properties of six strains of Acetobacter pasteurianus through nuclear factor-kappa B/activator protein-1 (NF-κB/AP-1) transcription factor activation and nitric oxide (NO) and cytokine production in macrophages. We found that the six A. pasteurianus strains had no significant inhibitory effect on the cell viability of RAW-Blue™ cells at the concentration of (25, 50, 100 CFU/macrophage). The production of NO and cytokines (TNF-α, IL-1β, and IL-6) showed different abilities of immune activation for each strain, and it was 0.7 to 0.9 times higher than that of the LPS (100 ng/mL, v/v) positive control and 7 to 8 times superior to that of the negative control group. To explore the underlying mechanism, we evaluated the mRNA expression of pro-inflammatory genes. Consequently, we found that inducible nitric oxide synthase (iNOS) and cyclooxygenase (COX)-2 expression including genes expression of cytokines were elevated by the six A. pasteurianus treatment. These results suggested that the six strains of A. pasteurianus have an excellent industrial application value as a functional material for the purpose of enhancing immune function.
在本研究中,我们通过核因子κB/活化蛋白-1(NF-κB/AP-1)转录因子激活以及巨噬细胞中一氧化氮(NO)和细胞因子的产生,研究了6株巴氏醋杆菌的免疫特性。我们发现6株A.pasteurianus菌株对RAW Blue的细胞活力没有显著的抑制作用™ 浓度为(25,50100CFU/巨噬细胞)的细胞。各菌株的NO和细胞因子(TNF-α、IL-1β和IL-6)的产生表现出不同的免疫激活能力,比LPS(100ng/mL,v/v)阳性对照组高0.7至0.9倍,比阴性对照组高7至8倍。为了探索潜在的机制,我们评估了促炎基因的mRNA表达。因此,我们发现诱导型一氧化氮合酶(iNOS)和环氧合酶(COX)-2的表达,包括细胞因子的基因表达,通过六种巴氏杆菌处理而升高。这些结果表明,6株巴氏杆菌作为增强免疫功能的功能材料具有良好的工业应用价值。
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引用次数: 0
Kiwi-persimmon wine produced using wild Saccharomyces cerevisiae strains with sugar, acid, and alcohol tolerance 用野生酿酒酵母生产的具有糖、酸和酒精耐受性的猕猴桃柿子酒
Q4 Agricultural and Biological Sciences Pub Date : 2023-02-01 DOI: 10.11002/kjfp.2023.30.1.52
H. Lee, K. Cho, O. Joo
100 different yeast colonies were isolated from spontaneously fermented kiwis, persimmons, apples, pears, watermelons, grapes, grape fruits, peachs, and plums, and selected yeast strains were used to produce kiwi-persimmon mixed wine (KPMW). Among the isolates, five representative strains exhibited tolerance to sucrose, alcohol, pH, and potassium metabisulfite when compared with the control yeast strain (Saccharomyces cerevisiae KCCM 12615). All five yeast strains (Y4, Y10, Y28, Y78, and Y81) exhibited 99% 26S rDNA sequence similarity to S. cerevisiae. The pH, acidity, Brix, reducing sugar, alcohol, and organic acid contents were consistent in KPMW prepared from the S. cerevisiae KCCM 12615 and Y28 strains. KPMW made from the Y4, Y10, and Y28 strains exhibited lower quantities of free sugars than those of the KPMW made from the other yeast strains. The level of ethyl esters in KPMW prepared from the Y28 was higher than that in the other KPMWs. All strains, except for Y28, produced lower concentrations of sulfur and ketone compounds. Furthermore, the KPMW produced by the Y28 strains had total phenolic contents with 1.1 g/L, with DPPH and ABTS radical scavenging activities of 57.06% and 55.62%, respectively, and a FRAP assay value of 0.72. Our results suggest that Y28 is a promising yeast strain for producing high-quality wines.
从自发发酵的猕猴桃、柿子、苹果、梨、西瓜、葡萄、葡萄、桃和李子中分离出100个不同的酵母菌落,并用筛选的酵母菌株生产猕猴桃-柿子混合酒(KPMW)。在分离物中,与对照酵母菌株(酿酒酵母KCCM 12615)相比,五个代表性菌株表现出对蔗糖、酒精、pH和偏亚硫酸钾的耐受性。所有五种酵母菌株(Y4、Y10、Y28、Y78和Y81)都表现出99%的26S rDNA序列与酿酒酵母的相似性。由酿酒酵母KCCM 12615和Y28菌株制备的KPMW中的pH、酸度、Brix、还原糖、醇和有机酸含量是一致的。由Y4、Y10和Y28菌株制成的KPMW表现出比由其它酵母菌株制成的那些KPMW更低量的游离糖。Y28制备的KPMW中的乙酯含量高于其他KPMW。除Y28外,所有菌株都产生较低浓度的硫和酮化合物。此外,Y28菌株产生的KPMW的总酚含量为1.1g/L,DPPH和ABTS自由基清除活性分别为57.06%和55.62%,FRAP测定值为0.72。我们的研究结果表明,Y28是一种生产优质葡萄酒的有前景的酵母菌株。
{"title":"Kiwi-persimmon wine produced using wild Saccharomyces\u0000 cerevisiae strains with sugar, acid, and alcohol\u0000 tolerance","authors":"H. Lee, K. Cho, O. Joo","doi":"10.11002/kjfp.2023.30.1.52","DOIUrl":"https://doi.org/10.11002/kjfp.2023.30.1.52","url":null,"abstract":"\u0000 \u0000 100 different yeast colonies were isolated from spontaneously fermented kiwis,\u0000 persimmons, apples, pears, watermelons, grapes, grape fruits, peachs, and plums,\u0000 and selected yeast strains were used to produce kiwi-persimmon mixed wine\u0000 (KPMW). Among the isolates, five representative strains exhibited tolerance to\u0000 sucrose, alcohol, pH, and potassium metabisulfite when compared with the control\u0000 yeast strain (Saccharomyces cerevisiae KCCM 12615). All five\u0000 yeast strains (Y4, Y10, Y28, Y78, and Y81) exhibited 99% 26S rDNA\u0000 sequence similarity to S. cerevisiae. The pH, acidity, Brix,\u0000 reducing sugar, alcohol, and organic acid contents were consistent in KPMW\u0000 prepared from the S. cerevisiae KCCM 12615 and Y28 strains.\u0000 KPMW made from the Y4, Y10, and Y28 strains exhibited lower quantities of free\u0000 sugars than those of the KPMW made from the other yeast strains. The level of\u0000 ethyl esters in KPMW prepared from the Y28 was higher than that in the other\u0000 KPMWs. All strains, except for Y28, produced lower concentrations of sulfur and\u0000 ketone compounds. Furthermore, the KPMW produced by the Y28 strains had total\u0000 phenolic contents with 1.1 g/L, with DPPH and ABTS radical scavenging activities\u0000 of 57.06% and 55.62%, respectively, and a FRAP assay value of\u0000 0.72. Our results suggest that Y28 is a promising yeast strain for producing\u0000 high-quality wines.\u0000","PeriodicalId":17875,"journal":{"name":"Korean Journal of Food Preservation","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42648933","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Evaluation of radical scavenging and diasestive enzyme inhibitory capacities of peach twigs fraction extract (Prunus persica L. Bastch) 桃枝部分提取物(Prunus persica L.Bastch)清除自由基和抑制一水硬脂酶能力的评价
Q4 Agricultural and Biological Sciences Pub Date : 2023-02-01 DOI: 10.11002/kjfp.2023.30.1.170
Youjeoung Lee, Gyeong Han Jeong, Ju-yeon Hong, Tae Hoon Kim
We investigated the free radical scavenging and digestive enzyme inhibitory activities of the hot water extract of peach twig (Prunus persica L. Bastch). This extract of the peach twigs was further split up into n-hexane, ethyl acetate (EtOAc), and n-butyl alcohol(n-BuOH), which resulted in three solvent-soluble fractions. Free radical scavenging activity was evaluated using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS+) assay systems, while hypoglycemic effect of the peach twig extract and the solvent-soluble fractions were tested using α-glucosidase and α-amylase inhibition assays. Accordingly, the EtOAc layer showed a greater free radical scavenging activity compared to other solvent-soluble fractions. Furthermore, based on the α-glucosidase and α-amylase assays, the IC50 values were determined to be 38.2±1.6 and 69.6±6.1 μg/mL for the EtOAc-soluble fractions, respectively. Taken together, these results suggest that the fractions obtained from the peach twig extract can be considered as a potential source of natural antioxidant and hypoglycaemic constituents.
研究了桃枝热水提取物对自由基的清除和对消化酶的抑制作用。将桃枝的提取物进一步分解为正己烷、乙酸乙酯(EtOAc)和正丁醇(n-BuOH),得到三种溶剂可溶的级分。使用2,2-二苯基-1-苦基肼(DPPH)和2,2'-叠氮基双(3-乙基苯并噻唑啉-6-磺酸)(ABTS+)测定系统评价了自由基清除活性,同时使用α-葡萄糖苷酶和α-淀粉酶抑制测定了桃枝提取物和溶剂可溶性组分的降血糖作用。因此,与其他溶剂可溶性组分相比,EtOAc层显示出更大的自由基清除活性。此外,基于α-葡萄糖苷酶和α-淀粉酶测定,EtOAc可溶性组分的IC50值分别为38.2±1.6和69.6±6.1μg/mL。总之,这些结果表明,从桃枝提取物中获得的组分可以被认为是天然抗氧化剂和低血糖成分的潜在来源。
{"title":"Evaluation of radical scavenging and diasestive enzyme inhibitory\u0000 capacities of peach twigs fraction extract (Prunus persica\u0000 L. Bastch)","authors":"Youjeoung Lee, Gyeong Han Jeong, Ju-yeon Hong, Tae Hoon Kim","doi":"10.11002/kjfp.2023.30.1.170","DOIUrl":"https://doi.org/10.11002/kjfp.2023.30.1.170","url":null,"abstract":"\u0000 \u0000 We investigated the free radical scavenging and digestive enzyme inhibitory\u0000 activities of the hot water extract of peach twig (Prunus\u0000 persica L. Bastch). This extract of the peach twigs was further\u0000 split up into n-hexane, ethyl acetate (EtOAc), and n-butyl\u0000 alcohol(n-BuOH), which resulted in three solvent-soluble\u0000 fractions. Free radical scavenging activity was evaluated using\u0000 2,2-diphenyl-1-picrylhydrazyl (DPPH) and\u0000 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)\u0000 (ABTS+) assay systems, while hypoglycemic effect of the\u0000 peach twig extract and the solvent-soluble fractions were tested using\u0000 α-glucosidase and α-amylase\u0000 inhibition assays. Accordingly, the EtOAc layer showed a greater free radical\u0000 scavenging activity compared to other solvent-soluble fractions. Furthermore,\u0000 based on the α-glucosidase and\u0000 α-amylase assays, the IC50 values were\u0000 determined to be 38.2±1.6 and 69.6±6.1\u0000 μg/mL for the EtOAc-soluble fractions, respectively.\u0000 Taken together, these results suggest that the fractions obtained from the peach\u0000 twig extract can be considered as a potential source of natural antioxidant and\u0000 hypoglycaemic constituents.\u0000","PeriodicalId":17875,"journal":{"name":"Korean Journal of Food Preservation","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47212614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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Korean Journal of Food Preservation
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