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Anti-oxidation, anti-inflammation, anti-wrinkle, and pore-tightening effects of phenolic compounds from Aeonium sedifolium leaves 莎草叶酚类化合物的抗氧化、抗炎、防皱和毛孔收紧作用
Q4 Agricultural and Biological Sciences Pub Date : 2023-04-01 DOI: 10.11002/kjfp.2023.30.2.347
Jung-In Kim, Min-Jae Kim, Hanna Jo, D. Jeong, Hye-Jin Park, Young-je Cho
The succulent plant Aeonium sedifolium leaves contain several compounds that are of interest for their cosmetic uses on the skin. This study measured the inhibitory effects of enzyme production and antioxidant, astringent effects and skin wrinkles using Aeonium sedifolium leaves (ASL). The total phenolics compounds (TPC) content of ASL under optimal extraction conditions was 34.49 mg/g for hot water extract (ASLW) and 61.64 mg/g for 50% ethanol extract (ASLE). The ASLW and ASLE extracts were freeze-dried, powdered, and used as solids. TPC content, 2,2-diphenyl-1-picrylhydrazy (DPPH) radical scavenging activity, and 2,2’-azinobis (3-ethylben-zothiazoline 6-sulfonate) (ABTS) radical inhibition of the ASL phenolics were tested. The DPPH radical scavenging activities of ASLW and ASLE were tested at a TPC of 100 μg/mL. ABTS radical inhibition showed antioxidant activity of 100.00% in ASLW and ASLE, and the antioxidant protection factor of ASLW and ASLE was 1.07 and 1.22, respectively. The thiobarbituric acid-reactive substance (TBARS) inhibitory activity of ASLW and ASLE was 77.00%. The elastase inhibitory activity of ASLE was 69.03%, and collagenase inhibition activity for ASLW and ASLE was 29.82% and 54.76%, respectively. The astringent effect of ASLE was 89.82% at a TPC of 200 μg/mL. Thus, we concluded that ASL has the potential as a functional cosmetic ingredient with anti-aging effects on the skin.
多肉植物sedifolium的叶子含有几种化合物,它们对皮肤的美容用途感兴趣。本研究测定了紫叶Aeonium sedifolium叶(ASL)对酶的产生、抗氧化、收敛和皮肤皱纹的抑制作用。在最佳提取条件下,热水提取液(ASLW)和50%乙醇提取液(ASLE)的总酚类化合物(TPC)含量分别为34.49 mg/g和61.64 mg/g。将ASLW和ASLE提取物冷冻干燥,制成粉末,用作固体。研究了TPC含量、2,2-二苯基-1-吡啶肼(DPPH)自由基清除能力和2,2 ' -氮化喹啉(3-乙基苯-并噻唑啉- 6-磺酸盐)(ABTS)自由基抑制能力。在TPC为100 μg/mL的条件下,检测ASLW和ASLE对DPPH自由基的清除能力。ABTS对ASLW和ASLE的抗氧化活性均为100.00%,抗氧化保护系数分别为1.07和1.22。硫代巴比妥酸反应物质(TBARS)对ASLW和ASLE的抑制活性为77.00%。ASLE的弹性酶抑制活性为69.03%,ASLW和ASLE的胶原酶抑制活性分别为29.82%和54.76%。在TPC为200 μg/mL时,ASLE的收敛效果为89.82%。因此,我们得出结论,ASL具有作为一种功能性化妆品成分的潜力,对皮肤具有抗衰老作用。
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引用次数: 0
Quality characteristics of different parts of garlic sprouts produced by smart farms during growth 智能农场生产的大蒜芽生长过程中不同部位的品质特征
Q4 Agricultural and Biological Sciences Pub Date : 2023-04-01 DOI: 10.11002/kjfp.2022.30.2.272
Yu-ri Choi, Su-Hwan Kim, Chae-Mi Lee, Dong-Hun Lee, Chae-Yun Lee, Hyeong-Woo Jo, Jaehoon Jeong, I. Oh, Ho-Kyung Ha, Jungsil Kim, C. Huh
Garlic sprouts can provide data on functional and food processing materials. This study compared the leaves, bulbs, and roots of garlic sprouts grown on smart farms during two growth periods (20 and 25 days). In addition, data for garlic bulbs grown in open fields were presented as reference materials. All garlic sprouts’ total free sugar content decreased as the growth period increased. All plant parts’ total organic acid content decreased as the growth period progressed, except for the root section. Potassium, phosphorus, and sulfur content increased during growth in all parts of the garlic sprouts. Alliin content decreased in all parts of the plant over time, whereas thiosulfinate content increased in the roots but decreased in the leaves and bulbs. Total polyphenol content increased in all parts of the plant during the growth period, except for the bulb, whereas the flavonoid content did not change significantly over time. The 2,2-diphenyl-1-picrylhydrazy (DPPH) and 2,2′-azinobis (3-ethylben-zothiazoline 6-sulfonate) (ABTS) free radical scavenging activities, as well as the superoxide dismutase (SOD)-like activity of garlic sprouts were 37.45-65.47%, 59.12-89.81%, and 89.52-98.59%, respectively. These activities tend to decrease during the growth period. Here, we showed that garlic sprouts have higher levels of functional substances and physiological activities than general garlic sprouts. It was also determined that a growth period of 20 days was suitable for garlic sprouts. Data for research on functional and food-processing materials can be obtained by analyzing garlic sprouts produced by smart farms.
蒜苗可以提供有关功能材料和食品加工材料的数据。这项研究比较了智能农场在两个生长期(20天和25天)种植的蒜苗的叶子、球茎和根部。此外,还提供了露地种植大蒜鳞茎的数据作为参考材料。所有蒜苗的总游离糖含量随着生长期的增加而降低。除根段外,所有植物部分的总有机酸含量均随生长期的进展而下降。钾、磷和硫的含量在蒜苗的生长过程中都有所增加。随着时间的推移,植株各部位的Alliin含量都有所下降,而硫代亚硫酸酯含量在根部增加,但在叶片和球茎中减少。在生长期间,除球茎外,植物所有部位的总多酚含量都增加了,而类黄酮含量没有随着时间的推移而显著变化。大蒜芽的2,2-二苯基-1-苦基肼(DPPH)和2,2′-叠氮双(3-乙基苯并噻唑啉-6-磺酸酯)(ABTS)自由基清除活性以及超氧化物歧化酶(SOD)样活性分别为37.45-65.47%、59.12-89.81%和89.52-98.59%。这些活动在生长期往往会减少。在这里,我们发现蒜苗比普通蒜苗具有更高水平的功能物质和生理活性。还确定了20天的生长期适合大蒜芽。通过分析智能农场生产的蒜苗,可以获得研究功能性和食品加工材料的数据。
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引用次数: 0
Effects of extraction conditions on color quality and antioxidant properties of persimmon (Diospyros kaki Thunb.) leaf tea 提取条件对柿叶茶颜色品质及抗氧化性能的影响
Q4 Agricultural and Biological Sciences Pub Date : 2023-04-01 DOI: 10.11002/kjfp.2022.30.2.300
Jong-Beom An, Juhae Kim, C. Kim
This study was carried out to investigate the effect of the extraction conditions for persimmon (Diospyros kaki Thunb.) leaf tea (PLT) on its color quality and antioxidant properties. The amount of persimmon leaf (PL) powder and pH influenced the PLT’s color and antioxidant capacity. As the amount of PL powder in tea increased, lightness decreased while yellowness increased. The PLT with the highest amount of PL (10 mg/mL) exhibited the highest 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging activity and ferric reducing antioxidant power (FRAP). In addition, the PLT with the highest PL showed the highest total polyphenol and flavonoid contents. Subsequently, PLT was prepared using 10 mg/mL PL powder under varying pH conditions. As pH increased from 4 to 7, lightness decreased while redness and yellowness increased. Antioxidant capacity was determined using 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity and FRAP assay showed that lower pH exhibited higher antioxidant capacity. The PLT extracted under the lowest pH of 4 showed higher polyphenol and flavonoid contents than that extracted under higher pH conditions. Overall, PLT extraction using a solvent with pH 4.0 showed better antioxidant activities and higher amounts of polyphenolic compounds. Simultaneously, lesser lightness, redness, and yellowness were detected in PLT extracted under pH 4 conditions. In conclusion, to acquire a better functional health benefit in terms of antioxidant capacity, preparing PLT under pH 4 conditions is suggested.
以柿叶茶为原料,研究了柿叶茶提取条件对其颜色品质和抗氧化性能的影响。柿叶粉的添加量和pH值影响了PLT的颜色和抗氧化能力。随着PL粉在茶叶中添加量的增加,茶叶的淡度降低,黄度增加。PL含量最高(10 mg/mL)的PLT具有最高的2,2′-氮基-双(3-乙基苯并噻唑-6-磺酸)自由基清除能力和铁还原抗氧化能力(FRAP)。此外,总多酚和类黄酮含量最高的PLT具有最高的PL值。随后,在不同的pH条件下,用10 mg/mL的PL粉末制备PLT。随着pH值从4增加到7,亮度降低,红、黄度增加。采用2,2-二苯基-1-苦味酰肼自由基清除活性测定抗氧化能力,FRAP测定结果表明,pH越低,抗氧化能力越强。在最低pH = 4条件下提取的枇杷叶提取物的多酚和类黄酮含量高于高pH条件下提取的枇杷叶提取物。综上所述,pH为4.0的溶剂提取物具有较好的抗氧化活性和较高的多酚类化合物含量。同时,在pH 4条件下提取的PLT的亮度、红色和黄色较小。综上所述,为了获得更好的抗氧化功能,建议在pH为4的条件下制备PLT。
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引用次数: 0
Vitamin contents and antioxidant characteristics of red and gold kimchi cabbages (Brassica rapa. L. ssp. pekinensis) 红色和金色泡菜白菜的维生素含量及抗氧化特性。l . ssp。学报)
Q4 Agricultural and Biological Sciences Pub Date : 2023-04-01 DOI: 10.11002/kjfp.2023.30.2.247
Kang-Hee Lee, S. Oh, Won-Ho Hong, Jiyeon Chun
Kimchi cabbage is widely consumed in Korea, with the popularity of this pickled vegetable dish growing internationally due to its health benefits. In this study, the physical (size, color), functional (antioxidant activity, total polyphenol, and flavonoid content), and nutritional (water- and fat-soluble vitamins) characteristics of two new kimchi cabbage varieties, namely red and gold kimchi cabbages (RKC and GKC, respectively), were analyzed and compared with those of the common kimchi cabbage (CKC). There were no significant differences in the thickness or length of the three kimchi cabbages, although RKC had the narrowest outer leaves among the three varieties (11.94 cm). Regarding chromaticity, yellowness was highest in GKC (29.86), whereas redness was highest in RKC (9.31). Furthermore, RKC had the highest recorded vitamin B6 and B9 (1,288.5 μg/100 g and 776.7 μg dietary folate equivalent/100 g, respectively). On the other hand, the fat-soluble vitamins vitamin A (β-carotene) and K (Phylloquinone) were both highest in GKC (907.1 μg/100 g and 712.2 μg/100 g, respectively). Generally, all kimchi cabbage samples contained high levels of vitamin E (1.8-4.9 mg α-tocopherol equivalent/100 g). RKC attained the highest antioxidant activity and total polyphenol and total flavonoid contents among the three kimchi cabbages. These results show that gold and red kimchi cabbage can be used as raw materials in the food-processing industry.
泡菜白菜在韩国被广泛食用,由于其有益健康,这种腌制蔬菜在国际上越来越受欢迎。本研究分析了两个泡菜白菜新品种——红白菜和金白菜(分别为RKC和GKC)的物理(大小、颜色)、功能(抗氧化活性、总多酚和类黄酮含量)和营养(水溶性维生素和脂溶性维生素)的特性,并与普通白菜(CKC)进行了比较。3种泡菜白菜的厚度和长度没有显著差异,但RKC的外叶最窄(11.94 cm)。色度方面,GKC黄度最高(29.86),RKC红度最高(9.31)。此外,RKC中维生素B6和B9的含量最高(分别为1,288.5 μg/100 g和776.7 μg/100 g)。另一方面,脂溶性维生素维生素A (β-胡萝卜素)和K(叶绿醌)在GKC中含量最高,分别为907.1 μg/100 g和712.2 μg/100 g。总体而言,3种泡菜样品的维生素E含量均较高(α-生育酚当量为1.8 ~ 4.9 mg /100 g),其中RKC的抗氧化活性、总多酚和总黄酮含量最高。这些结果表明,金色和红色的泡菜白菜可以作为食品加工工业的原料。
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引用次数: 0
Quality characteristics of in vitro luwak coffee produced using enzyme and microbial complexes 酶和微生物复合物制备离体羽扇豆咖啡的质量特性
Q4 Agricultural and Biological Sciences Pub Date : 2023-04-01 DOI: 10.11002/kjfp.2022.30.2.287
HyeonCheol Kang, S. Oh, H. Kang, J. Kwon, Y. Jeong
In vitro luwak coffee was produced using enzyme-microbial complexes. The coffee quality of non-fermented coffee beans (NFC) and fermented coffee beans (FC) was compared. The total free amino acid content was higher in FC than in NFC. The levels of glutamic acid and γ-amino-n-butyric acid in NFC were higher than those in FC; however, the contents of essential amino acids, such as lysine, leucine, and valine, in FC were higher than in NFC. During fermentation, the sucrose content decreased, whereas the fructose and glucose contents increased (p<0.001). The chromaticity of the coffee extract showed higher lightness (L), redness (a), and yellowness (b) values in FC than those in NFC. The caffeine content was significantly lower in FC (696.94±0.04 μg/mL) compared to that in NFC (1,130.22±1.55 μg/mL) (p<0.001). Conversely, the polyphenol and chlorogenic acid contents were significantly higher ​​in NFC than in FC (p<0.001). Electronic nose analysis indicated considerable differences between the volatile aromatic components in NFC and FC. Sensory scores were significantly higher for FC than those for NFC. Therefore, the fermentation of coffee beans using enzyme-microbial complexes altered the chemical components, which promoted the Maillard reaction during the coffee bean roasting process. These results suggest the possibility of producing in vitro luwak coffee with better flavor and lower caffeine content.
利用酶-微生物复合物在体外制备luwak咖啡。比较了未发酵咖啡豆(NFC)和发酵咖啡豆(FC)的咖啡品质。游离氨基酸总含量FC高于NFC。NFC中谷氨酸和γ-氨基正丁酸含量高于FC;然而,FC中必需氨基酸如赖氨酸、亮氨酸和缬氨酸的含量高于NFC。发酵过程中,蔗糖含量降低,果糖和葡萄糖含量升高(p<0.001)。与NFC相比,FC中咖啡提取物的亮度(L)、红度(a)和黄度(b)值更高。FC中咖啡因含量(696.94±0.04 μg/mL)显著低于NFC(1130.22±1.55 μg/mL) (p<0.001)。相反,NFC的多酚和绿原酸含量显著高于FC (p<0.001)。电子鼻分析表明,NFC和FC的挥发性芳香成分存在较大差异。FC组的感觉评分明显高于NFC组。因此,利用酶-微生物复合物对咖啡豆进行发酵,改变了咖啡豆的化学成分,促进了咖啡豆烘焙过程中的美拉德反应。这些结果表明,有可能生产出风味更好、咖啡因含量更低的体外luwak咖啡。
{"title":"Quality characteristics of in vitro luwak coffee produced using enzyme and microbial complexes","authors":"HyeonCheol Kang, S. Oh, H. Kang, J. Kwon, Y. Jeong","doi":"10.11002/kjfp.2022.30.2.287","DOIUrl":"https://doi.org/10.11002/kjfp.2022.30.2.287","url":null,"abstract":"\u0000\u0000In vitro luwak coffee was produced using enzyme-microbial complexes. The coffee quality of non-fermented coffee beans (NFC) and fermented coffee beans (FC) was compared. The total free amino acid content was higher in FC than in NFC. The levels of glutamic acid and γ-amino-n-butyric acid in NFC were higher than those in FC; however, the contents of essential amino acids, such as lysine, leucine, and valine, in FC were higher than in NFC. During fermentation, the sucrose content decreased, whereas the fructose and glucose contents increased (p<0.001). The chromaticity of the coffee extract showed higher lightness (L), redness (a), and yellowness (b) values in FC than those in NFC. The caffeine content was significantly lower in FC (696.94±0.04 μg/mL) compared to that in NFC (1,130.22±1.55 μg/mL) (p<0.001). Conversely, the polyphenol and chlorogenic acid contents were significantly higher ​​in NFC than in FC (p<0.001). Electronic nose analysis indicated considerable differences between the volatile aromatic components in NFC and FC. Sensory scores were significantly higher for FC than those for NFC. Therefore, the fermentation of coffee beans using enzyme-microbial complexes altered the chemical components, which promoted the Maillard reaction during the coffee bean roasting process. These results suggest the possibility of producing in vitro luwak coffee with better flavor and lower caffeine content.\u0000","PeriodicalId":17875,"journal":{"name":"Korean Journal of Food Preservation","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45555158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Isolation and characterization of a lytic Salmonella Typhimurium-specific phage as a potential biofilm control agent 鼠伤寒沙门菌特异性噬菌体的分离与特性研究
Q4 Agricultural and Biological Sciences Pub Date : 2023-02-01 DOI: 10.11002/kjfp.2023.30.1.42
Suhui Kim, Mi-Kyung Park
This study aimed to characterize a lytic Salmonella Typhimurium-specific (ST) phage and its biofilm control capability against S. Typhimurium biofilm on polypropylene surface. ST phage was isolated, propagated, and purified from water used in a slaughterhouse. The morphology of ST phage was observed via transmission electron microscopy. Its bactericidal effect was evaluated by determining bacterial concentrations after the phage treatment at various multiplicities of infection (MOIs) of 0.01, 1.0, and 100. Once the biofilm was formed on the polypropylene tube after incubation at 37°C for 48 h, the phage was treated and its antibiofilm capability was determined using crystal violet staining and plate count method. The phage was isolated and purified at a final concentration of ∼11 log PFU/mL. It was identified as a myophage with an icosahedral head (∼104 nm) and contractile tail (∼90-115 nm). ST phage could significantly decrease S. Typhimurium population by ∼2.8 log CFU/mL at an MOI of 100. After incubation for 48 h, biofilm formation on polypropylene surface was confirmed with a bacterial population of ∼6.9 log CFU/cm2. After 1 h treatment with ST phage, the bacterial population in the biofilm was reduced by 2.8 log CFU/cm2. Therefore, these results suggest that lytic ST phage as a promising biofilm control agent for eradicating S. Typhimurium biofilm formed on food contact surfaces.
本研究旨在表征裂解鼠伤寒沙门氏菌特异性(ST)噬菌体及其对聚丙烯表面鼠伤寒沙门氏菌生物膜的生物膜控制能力。从屠宰场使用的水中分离、繁殖和纯化ST噬菌体。用透射电镜观察了ST噬菌体的形态。通过测定噬菌体处理后在0.01、1.0和100的各种感染倍数(MOI)下的细菌浓度来评估其杀菌效果。在37°C下孵育48小时后,一旦在聚丙烯管上形成生物膜,就对噬菌体进行处理,并使用结晶紫染色和平板计数法测定其抗生物膜能力。分离并纯化噬菌体,最终浓度为~11 log PFU/mL。它被鉴定为一种具有二十面体头部(~104 nm)和可收缩尾部(~90-115 nm)的肌噬。在MOI为100时,ST噬菌体可显著减少鼠伤寒沙门氏菌种群约2.8 log CFU/mL。培养48小时后,聚丙烯表面上的生物膜形成得到证实,细菌群为~6.9 log CFU/cm2。用ST噬菌体处理1小时后,生物膜中的细菌种群减少了2.8 log CFU/cm2。因此,这些结果表明,裂解ST噬菌体是一种很有前途的生物膜控制剂,可以根除在食物接触表面形成的鼠伤寒杆菌生物膜。
{"title":"Isolation and characterization of a lytic Salmonella\u0000 Typhimurium-specific phage as a potential biofilm control agent","authors":"Suhui Kim, Mi-Kyung Park","doi":"10.11002/kjfp.2023.30.1.42","DOIUrl":"https://doi.org/10.11002/kjfp.2023.30.1.42","url":null,"abstract":"\u0000 \u0000 This study aimed to characterize a lytic Salmonella\u0000 Typhimurium-specific (ST) phage and its biofilm control capability against\u0000 S. Typhimurium biofilm on polypropylene surface. ST phage\u0000 was isolated, propagated, and purified from water used in a slaughterhouse. The\u0000 morphology of ST phage was observed via transmission electron microscopy. Its\u0000 bactericidal effect was evaluated by determining bacterial concentrations after\u0000 the phage treatment at various multiplicities of infection (MOIs) of 0.01, 1.0,\u0000 and 100. Once the biofilm was formed on the polypropylene tube after incubation\u0000 at 37°C for 48 h, the phage was treated and its antibiofilm capability\u0000 was determined using crystal violet staining and plate count method. The phage\u0000 was isolated and purified at a final concentration of ∼11 log PFU/mL. It was\u0000 identified as a myophage with an icosahedral head (∼104 nm) and contractile tail\u0000 (∼90-115 nm). ST phage could significantly decrease S.\u0000 Typhimurium population by ∼2.8 log CFU/mL at an MOI of 100. After incubation for\u0000 48 h, biofilm formation on polypropylene surface was confirmed with a bacterial\u0000 population of ∼6.9 log CFU/cm2. After 1 h treatment with ST phage,\u0000 the bacterial population in the biofilm was reduced by 2.8 log\u0000 CFU/cm2. Therefore, these results suggest that lytic ST phage as\u0000 a promising biofilm control agent for eradicating S.\u0000 Typhimurium biofilm formed on food contact surfaces.\u0000","PeriodicalId":17875,"journal":{"name":"Korean Journal of Food Preservation","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45137156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effects of 1-methylcyclopropene (1-MCP) treatment on the maintenance of fruit quality of RubyS apples during cold storage 1-甲基环丙烯(1-MCP)处理对红宝石苹果冷藏期果实品质维持的影响
Q4 Agricultural and Biological Sciences Pub Date : 2023-02-01 DOI: 10.11002/kjfp.2023.30.1.78
Jingi Yoo, Hwajong Yoo, N. M. Win, H. Jung, Young-je Cho, I. Kang
This study was conducted to evaluate the effect of different 1-methylcyclopropene (1-MCP) concentrations on fruit quality of small-sized RubyS apples during cold storage. After harvesting, the fruits were treated with 1-MCP at 0.5 or 1 μL/L concentrations and, subsequently, stored at 0°C for 6 months. After 6 months, the flesh firmness of untreated fruits, which was 85.4 N at harvest, had gradually decreased to 46.5 N; however, that of 1-MCP-treated fruits was maintained at 59.1 and 59.5 N. Titratable acidity (TA) of untreated fruits had also decreased from 0.42 to 0.24%, whereas that of 1-MCP-treated fruits was maintained at 0.26 and 0.27%. Soluble solids content (SSC) did not differ in all fruits. However, the 1-MCP-treated fruits had lower levels of SSC/TA ratio than untreated fruits. Moreover, after 6 months, the ethylene production had increased to 47.0 μL/kg/h in the untreated fruits, whereas 1-MCP blocked the ethylene production at 1.4 and 1.7 μL/kg/h. The weight loss and peel color variables remained unaffected by 1-MCP treatments. Thus, these results indicated that, for RubyS apples, the storability was only 2 months at 0°C without treatment, which can be extended to 6 months with 1-MCP treatments. The application of 1-MCP at 0.5 μL/L concentration is effectively and economically sufficient to maintain the quality of RubyS apples.
本试验研究了不同浓度的1-甲基环丙烯(1-MCP)对小型红宝石苹果冷藏期间果实品质的影响。收获后,用0.5或1 μL/L浓度的1- mcp处理果实,然后在0°C下保存6个月。6个月后,未处理果实的果肉硬度由收获时的85.4 N逐渐降低到46.5 N;但1- mcp处理的果实可滴定酸度(TA)维持在59.1和59.5 n,未处理的果实可滴定酸度(TA)也从0.42降至0.24%,而1- mcp处理的果实可滴定酸度维持在0.26和0.27%。可溶性固形物含量(SSC)在所有果实中没有差异。然而,1- mcp处理的果实的SSC/TA比值低于未处理的果实。此外,6个月后,未经处理的果实乙烯产量增加到47.0 μL/kg/h,而1-MCP抑制了1.4和1.7 μL/kg/h的乙烯产量。体重减轻和果皮颜色变量不受1-MCP处理的影响。综上所述,未经处理的红宝石苹果在0°C下的贮藏期仅为2个月,而经1-MCP处理后可延长至6个月。应用浓度为0.5 μL/L的1-MCP可有效、经济地保持红宝石苹果的品质。
{"title":"Effects of 1-methylcyclopropene (1-MCP) treatment on the\u0000 maintenance of fruit quality of RubyS apples during cold\u0000 storage","authors":"Jingi Yoo, Hwajong Yoo, N. M. Win, H. Jung, Young-je Cho, I. Kang","doi":"10.11002/kjfp.2023.30.1.78","DOIUrl":"https://doi.org/10.11002/kjfp.2023.30.1.78","url":null,"abstract":"\u0000 \u0000 This study was conducted to evaluate the effect of different 1-methylcyclopropene\u0000 (1-MCP) concentrations on fruit quality of small-sized RubyS\u0000 apples during cold storage. After harvesting, the fruits were treated with 1-MCP\u0000 at 0.5 or 1 μL/L concentrations and, subsequently,\u0000 stored at 0°C for 6 months. After 6 months, the flesh firmness of\u0000 untreated fruits, which was 85.4 N at harvest, had gradually decreased to 46.5\u0000 N; however, that of 1-MCP-treated fruits was maintained at 59.1 and 59.5 N.\u0000 Titratable acidity (TA) of untreated fruits had also decreased from 0.42 to\u0000 0.24%, whereas that of 1-MCP-treated fruits was maintained at 0.26 and\u0000 0.27%. Soluble solids content (SSC) did not differ in all fruits.\u0000 However, the 1-MCP-treated fruits had lower levels of SSC/TA ratio than\u0000 untreated fruits. Moreover, after 6 months, the ethylene production had\u0000 increased to 47.0 μL/kg/h in the untreated fruits,\u0000 whereas 1-MCP blocked the ethylene production at 1.4 and 1.7\u0000 μL/kg/h. The weight loss and peel color variables\u0000 remained unaffected by 1-MCP treatments. Thus, these results indicated that, for\u0000 RubyS apples, the storability was only 2 months at\u0000 0°C without treatment, which can be extended to 6 months with 1-MCP\u0000 treatments. The application of 1-MCP at 0.5 μL/L\u0000 concentration is effectively and economically sufficient to maintain the quality\u0000 of RubyS apples.\u0000","PeriodicalId":17875,"journal":{"name":"Korean Journal of Food Preservation","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41445397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Method validation for quantitative analyzing aflatoxin productivity in Aspergillus sp. isolated from soybean paste 从大豆酱中分离的曲霉产黄曲霉毒素的方法验证
Q4 Agricultural and Biological Sciences Pub Date : 2023-02-01 DOI: 10.11002/kjfp.2023.30.1.28
S. Yoo, W. Jeong, S. Yeo, So-Young Kim
Non-aflatoxigenic Aspergillus oryzae and aflatoxigenic A. flavus cannot be clearly identified by partial sequencing of the internal transcribed spacer (ITS) and 18S ribosomal ribonucleic acid (18S rRNA) regions. This study aimed to compare the accuracy among three aflatoxin detection methods using ultra-performance liquid chromatography (UPLC), high-performance liquid chromatography (HPLC), and an enzyme-linked immunosorbent assay (ELISA) kit and to select the non-aflatoxigenic Aspergillus sp. isolated from soybean paste. All analytical methods were suitable according to the international standards of Codex Alimentarius FAO-WHO (CODEX) or the Ministry of Food and Drug Safety (MFDS). UPLC exhibited the best of limit of detection (LOD) and limit of quantification (LOQ). Based on UPLC, HPLC, and the ELISA kit assay, the P5 and P7 strains isolated from soybean paste had 1,663.49, 1,468.12, and ⟩20 μg/kg and 1,470.08, 1,056.73, and ⟩20 μg/kg, respectively, detected and re-identified as A. flavus. In contrast, the P3 and P4 strains (A. oryzae), which were detected below the MFDS standards in all assays, were confirmed as non-aflatoxigenic fungi. Among the methods evaluated for quantitative analysis of aflatoxin, UPLC and HPLC are superior in terms of accuracy, and the ELISA kit rapidly detects low concentrations of aflatoxin. Furthermore, this study demonstrates that any Aspergillus sp. isolated for use as a fermentation starter should be analyzed for potential aflatoxin production using UPLC and HPLC for accurate quantitative analysis or ELISA for the rapid detection of low-level concentrations of aflatoxin.
非黄曲霉米曲霉和黄曲霉不能通过内部转录间隔区(ITS)和18S核糖体核糖核酸(18S rRNA)区域的部分测序来明确鉴定。本研究旨在比较超高效液相色谱法(UPLC)、高效液相色谱法(HPLC)和酶联免疫吸附法(ELISA)检测黄曲霉毒素的准确性,并筛选出从大豆酱中分离的非黄曲霉。所有分析方法均符合食品法典FAO-WHO (Codex)或食品药品安全部(MFDS)的国际标准。UPLC具有最佳的检出限和定量限。基于UPLC, HPLC和ELISA试剂盒测定,从大豆酱中分离的P5和P7菌株分别检测到1,663.49,1,468.12和⟩20 μg/kg和1,470.08,1,056.73和⟩20 μg/kg,并重新鉴定为A. flavus。相比之下,P3和P4菌株(A. oryzae)的检测结果均低于MFDS标准,证实为非黄曲霉毒素。在评价的黄曲霉毒素定量分析方法中,UPLC和HPLC的准确性较好,ELISA试剂盒可快速检测低浓度黄曲霉毒素。此外,本研究表明,任何分离的用作发酵剂的曲霉都应该使用UPLC和HPLC进行准确的定量分析,或者使用ELISA快速检测低浓度的黄曲霉毒素。
{"title":"Method validation for quantitative analyzing aflatoxin productivity\u0000 in Aspergillus sp. isolated from soybean paste","authors":"S. Yoo, W. Jeong, S. Yeo, So-Young Kim","doi":"10.11002/kjfp.2023.30.1.28","DOIUrl":"https://doi.org/10.11002/kjfp.2023.30.1.28","url":null,"abstract":"\u0000 \u0000 Non-aflatoxigenic Aspergillus oryzae and aflatoxigenic\u0000 A. flavus cannot be clearly identified by partial\u0000 sequencing of the internal transcribed spacer (ITS) and 18S ribosomal\u0000 ribonucleic acid (18S rRNA) regions. This study aimed to compare the accuracy\u0000 among three aflatoxin detection methods using ultra-performance liquid\u0000 chromatography (UPLC), high-performance liquid chromatography (HPLC), and an\u0000 enzyme-linked immunosorbent assay (ELISA) kit and to select the\u0000 non-aflatoxigenic Aspergillus sp. isolated from soybean paste.\u0000 All analytical methods were suitable according to the international standards of\u0000 Codex Alimentarius FAO-WHO (CODEX) or the Ministry of Food and Drug Safety\u0000 (MFDS). UPLC exhibited the best of limit of detection (LOD) and limit of\u0000 quantification (LOQ). Based on UPLC, HPLC, and the ELISA kit assay, the P5 and\u0000 P7 strains isolated from soybean paste had 1,663.49, 1,468.12, and ⟩20\u0000 μg/kg and 1,470.08, 1,056.73, and ⟩20\u0000 μg/kg, respectively, detected and re-identified as\u0000 A. flavus. In contrast, the P3 and P4 strains (A.\u0000 oryzae), which were detected below the MFDS standards in all\u0000 assays, were confirmed as non-aflatoxigenic fungi. Among the methods evaluated\u0000 for quantitative analysis of aflatoxin, UPLC and HPLC are superior in terms of\u0000 accuracy, and the ELISA kit rapidly detects low concentrations of aflatoxin.\u0000 Furthermore, this study demonstrates that any Aspergillus sp.\u0000 isolated for use as a fermentation starter should be analyzed for potential\u0000 aflatoxin production using UPLC and HPLC for accurate quantitative analysis or\u0000 ELISA for the rapid detection of low-level concentrations of aflatoxin.\u0000","PeriodicalId":17875,"journal":{"name":"Korean Journal of Food Preservation","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45431393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Optimization of biomass production of Acetobacter pasteurianus SRCM101388 巴氏醋杆菌SRCM101388生物量生产的优化
Q4 Agricultural and Biological Sciences Pub Date : 2023-02-01 DOI: 10.11002/kjfp.2023.30.1.132
Jun-Tae Kim, Sung-Ho Cho, Do-Youn Jeong, Young-Min Kim
In this study, culture conditions were optimized to confirm the feasibility of Acetobacter pasteurianus as a starter for fermentation vinegar. Acetobacter pasteurianus strain can be used as a food ingredient. The optimal temperature and pH conditions of the selected Acetobacter pasteurianus SRCM101388 were 28°C and pH 6.00, respectively. The response surface methodology (RSM) was used to optimize the composition of the medium, and Plackett-Burman design (PBD) was used to obtain the effective selection of culture medium, resulting in that glucose, sucrose, and yeast extract had the highest effect on increasing biomass. The optimal concentration, which was performed by central composite design (CCD), were determined to be 10.73 g/L of glucose, 3.98 g/L of sucrose, and 18.73 g/L of yeast extract, respectively. The optimal concentrations of trace elements for the production of biomass were found to be 1 g/L of ammonium sulfate, 0.5 g/L of magnesium sulfate, 2 g/L of sodium phosphate monobasic, 2 g/L of sodium phosphate dibasic, and the final optimized medium was pH 6.10. When incubated in a 5 L jar fermenter, the SRCM101388 strain showed a faster-dissolved oxygen (DO) reduction at a lower agitation rate (rpm), and it was able to grow even at reduced DO level when aeration was maintained. The amount of final biomass produced was 2.53±0.12×109CFU/mL (9.40±0.02 log CFU/mL) when incubated for 18 hours at 150 rpm, 0.5 vvm, pH 6.0, and 28°C.
本研究对培养条件进行了优化,以证实巴氏醋杆菌作为发酵醋的发酵剂的可行性。巴氏醋杆菌菌株可用作食品原料。筛选出的巴氏醋杆菌SRCM101388的最适温度和pH条件分别为28°C和6.00。采用响应面法(RSM)对培养基的组成进行优化,并采用Plackett-Burman设计(PBD)对培养液进行有效筛选,结果表明葡萄糖、蔗糖和酵母提取物对提高生物量的作用最大。通过中心复合设计(CCD)确定的最佳浓度分别为10.73 g/L葡萄糖、3.98 g/L蔗糖和18.73 g/L酵母提取物。生产生物质的最佳微量元素浓度为1 g/L硫酸铵、0.5 g/L硫酸镁、2 g/L磷酸二氢钠、2 g/L磷酸二钠,最终优化培养基为pH 6.10。当在5L罐式发酵罐中培养时,SRCM101388菌株在较低的搅拌速率(rpm)下表现出更快的溶解氧(DO)还原,并且当保持通气时,即使在降低的DO水平下也能够生长。当在150 rpm、0.5 vvm、pH 6.0和28°C下孵育18小时时,最终产生的生物量为2.53±0.12×109CFU/mL(9.40±0.02 log CFU/mL)。
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引用次数: 0
Fermentation characteristics of yakju containing different amounts of steam-cooked Jerusalem artichoke (Helianthus tuberosus L.) 蒸制菊芋(Helianthus tuberosus L.)的发酵特性
Q4 Agricultural and Biological Sciences Pub Date : 2023-02-01 DOI: 10.11002/kjfp.2023.30.1.155
Jun-Su Choi, Kyu-Taek Choi, C. Kim, Heui-Dong Park, Sae-Byuk Lee
Jerusalem artichoke (JA, Helianthus tuberosus L.) has a great potential to enhance the quality of yakju due to the plentiful inulin content which is functional and indigestible carbohydrate in human. In this study, the optimal preparation conditions such as the added amount and steam treatment of JA were investigated to improve the quality of yakju. As the amount of JA added to yakju increased, alcohol production decreased, whereas fermentation was performed well when the steam-cooked JA was added to yakju. The pH and total acidity of yakju decreased and increased, respectively, when the amount of JA added to yakju increased, whereas pH and total acidity of yakju increased and decreased, respectively, when the steam-cooked JA was added to yakju. The free sugar and organic acid contents of yakju increased and decreased, respectively, when the amount of JA added to yakju increased, whereas those of yakju decreased when the steam-cooked JA was added to Yakju. Amino acid content of JA decreased as the amount of JA added to yakju increased and that of JA significantly decreased when the steam-cooked JA was added to yakju. In the sensory evaluation analysis, the addition of 10% unsteam-cooked JA to yakju was the best when considering sweetness, flavor, sourness, and overall preference of yakju supplemented with JA. Consequently, utilizing JA to yakju may contribute to the improvement of the quality of yakju.
菊芋(JA, Helianthus tuberosus L.)中含有丰富的功能性碳水化合物菊粉,具有提高酒酒品质的潜力。为了提高酒酒的质量,研究了JA的添加量和蒸汽处理等最佳制备条件。随着酒酒中JA添加量的增加,酒精产量下降,而将蒸熟的JA添加到酒酒中发酵效果良好。随着JA添加量的增加,酒酒的pH值和总酸度分别升高和降低,而随着JA添加量的增加,酒酒的pH值和总酸度分别升高和降低。随着JA用量的增加,酒酒的游离糖和有机酸含量分别增加和减少,而随着JA的添加,酒酒的游离糖和有机酸含量减少。随着JA添加量的增加,JA的氨基酸含量降低,蒸熟JA添加量显著降低。在感官评价分析中,从酒酒的甜度、风味、酸味和整体偏好等方面考虑,添加10%未蒸熟JA的酒酒效果最好。因此,在酒酒中应用JA可以提高酒酒的品质。
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引用次数: 0
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Korean Journal of Food Preservation
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