{"title":"Evaluation of different methods for the effective isolation of high-quality DNA from rodent faecal samples","authors":"Asri, N. A. A. N. M., Amin, Z., Wei, Y. C.","doi":"10.21161/mjm.230005","DOIUrl":"https://doi.org/10.21161/mjm.230005","url":null,"abstract":"","PeriodicalId":18178,"journal":{"name":"Malaysian Journal of Microbiology","volume":"557 ","pages":""},"PeriodicalIF":0.3,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139020120","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Genomic analysis of endophytic Bacillus altitudinis strain VUMS1 from Kappaphycus alvarezii and its inhibitory effect against Vibrio parahaemolyticus","authors":"Mangun, V. V., Yong, W. T. L., Yusof, N. A.","doi":"10.21161/mjm.230001","DOIUrl":"https://doi.org/10.21161/mjm.230001","url":null,"abstract":"","PeriodicalId":18178,"journal":{"name":"Malaysian Journal of Microbiology","volume":"56 ","pages":""},"PeriodicalIF":0.3,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139016576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Packaging systems for generating SARS-CoV-2 pseudoviruses: A mini review","authors":"Chin, J. W. T., Budiman, C., Teoh, P. L.","doi":"10.21161/mjm.230026","DOIUrl":"https://doi.org/10.21161/mjm.230026","url":null,"abstract":"","PeriodicalId":18178,"journal":{"name":"Malaysian Journal of Microbiology","volume":"620 ","pages":""},"PeriodicalIF":0.3,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139023502","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Husna Zulkipli, Hifa Nazirah, M. Yaziz, M. Idorus, Siti Hamimah Sheikh, Abdul Kadir, M. Musa, Khalilah Abdul Khalil
Aims: This study aimed to investigate the effect of exopolysaccharide (EPS) from Bifidobacterium pseudocatenulatum ATCC 27919 in Caco-2 cells on apoptotic and autophagic pathways. Methodology and results: Cell viability was examined by MTS assay and it showed a significant decrease in Caco-2 cells after exposure to EPS. Microscopy imaging and morphological analyses demonstrated that EPS-exposed Caco-2 cells exhibited the main morphological characteristics of apoptosis. EPS-exposed cells showed early apoptosis and cell cycle arrest at the G2/M phase in the cell cycle and dead cell assays. qPCR suggested that mRNA expression of substantial apoptotic markers such as cleaved Caspase-3, BAX and PARP-1 were significantly increased in EPS-exposed cells. The autophagy event was demonstrated in EPS-exposed cells by the contrary mRNA expression of Beclin-1 and Bcl-2 and the detection of autophagic LC3-II protein at 24 h exposure. GRP78 mRNA expression was also increased in EPS-exposed cells, indicating the occurrence of endoplasmic reticulum (ER) stress. Conclusion, significance and impact of study: Autophagy activity in EPS-exposed Caco-2 cells preceded apoptosis, suggesting it was a cytoprotective response against ER stress. Research findings set the foundation for therapeutic CRC treatments and provide insight into its regulatory processes.
{"title":"Bifidobacterium pseudocatenulatum ATCC 27919 exopolysaccharides induced autophagy and apoptosis against endoplasmic reticulum stress in Caco-2 cells","authors":"Husna Zulkipli, Hifa Nazirah, M. Yaziz, M. Idorus, Siti Hamimah Sheikh, Abdul Kadir, M. Musa, Khalilah Abdul Khalil","doi":"10.21161/mjm.230246","DOIUrl":"https://doi.org/10.21161/mjm.230246","url":null,"abstract":"Aims: This study aimed to investigate the effect of exopolysaccharide (EPS) from Bifidobacterium pseudocatenulatum ATCC 27919 in Caco-2 cells on apoptotic and autophagic pathways. Methodology and results: Cell viability was examined by MTS assay and it showed a significant decrease in Caco-2 cells after exposure to EPS. Microscopy imaging and morphological analyses demonstrated that EPS-exposed Caco-2 cells exhibited the main morphological characteristics of apoptosis. EPS-exposed cells showed early apoptosis and cell cycle arrest at the G2/M phase in the cell cycle and dead cell assays. qPCR suggested that mRNA expression of substantial apoptotic markers such as cleaved Caspase-3, BAX and PARP-1 were significantly increased in EPS-exposed cells. The autophagy event was demonstrated in EPS-exposed cells by the contrary mRNA expression of Beclin-1 and Bcl-2 and the detection of autophagic LC3-II protein at 24 h exposure. GRP78 mRNA expression was also increased in EPS-exposed cells, indicating the occurrence of endoplasmic reticulum (ER) stress. Conclusion, significance and impact of study: Autophagy activity in EPS-exposed Caco-2 cells preceded apoptosis, suggesting it was a cytoprotective response against ER stress. Research findings set the foundation for therapeutic CRC treatments and provide insight into its regulatory processes.","PeriodicalId":18178,"journal":{"name":"Malaysian Journal of Microbiology","volume":"199 1","pages":""},"PeriodicalIF":0.3,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75982196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
None Allu, M. A., None Yaseen, F. D., None Khathayer, F. H., None Assafi, M. S.
{"title":"Isolation and identification of pathogenic bacterial species from public hospital patients at Zakho and Duhok cities","authors":"None Allu, M. A., None Yaseen, F. D., None Khathayer, F. H., None Assafi, M. S.","doi":"10.21161/mjm.230223","DOIUrl":"https://doi.org/10.21161/mjm.230223","url":null,"abstract":"","PeriodicalId":18178,"journal":{"name":"Malaysian Journal of Microbiology","volume":"3 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135170450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Amalia, M., Kemit, G. M. N. B., Kahfi, M., Syahputra, A., Ervina, I., Nasution, R. O.
{"title":"In vitro evaluation of Andaliman (Zanthoxylum acanthopodium DC) extract on the growth of periodontal pathogenic bacteria","authors":"Amalia, M., Kemit, G. M. N. B., Kahfi, M., Syahputra, A., Ervina, I., Nasution, R. O.","doi":"10.21161/mjm.220110","DOIUrl":"https://doi.org/10.21161/mjm.220110","url":null,"abstract":"","PeriodicalId":18178,"journal":{"name":"Malaysian Journal of Microbiology","volume":"60 1","pages":""},"PeriodicalIF":0.3,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87020964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
None Abdul Halim, M., None Chai, C. N., None Yam, H. C., None Rosli, N., None Azlan, A., None Mohamad, F., None Azzam, G., None Najimudin, N.
{"title":"Transcriptomic response of an Antarctic yeast Rhodotorula sp. USM-PSY62 to temperature changes","authors":"None Abdul Halim, M., None Chai, C. N., None Yam, H. C., None Rosli, N., None Azlan, A., None Mohamad, F., None Azzam, G., None Najimudin, N.","doi":"10.21161/mjm.220053","DOIUrl":"https://doi.org/10.21161/mjm.220053","url":null,"abstract":"","PeriodicalId":18178,"journal":{"name":"Malaysian Journal of Microbiology","volume":"44 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135170451","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Prastiyanto, M. E., Retnaningrum, E., Darmawati, S., Aji, M. Z. B., Putri, D. R., Khairunnisa, A., Arilya, N. Y., Wulansari, K. P., Qoni’ah, U. H.
{"title":"Anti-MDR bacterial activity of bacteria associated with sea sponge of Amphimedon sp. from Karimunjawa Island, Central Java, Indonesia","authors":"Prastiyanto, M. E., Retnaningrum, E., Darmawati, S., Aji, M. Z. B., Putri, D. R., Khairunnisa, A., Arilya, N. Y., Wulansari, K. P., Qoni’ah, U. H.","doi":"10.21161/mjm.230195","DOIUrl":"https://doi.org/10.21161/mjm.230195","url":null,"abstract":"","PeriodicalId":18178,"journal":{"name":"Malaysian Journal of Microbiology","volume":"18 1","pages":""},"PeriodicalIF":0.3,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78805508","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Isolation and molecular identification of ethanol-tolerant Acetobacter species from Lansium domesticum (Dokong) and Nephelium lappaceum (Rambutan) vinegar","authors":"None Mansor, A. B., None Mokhtar, S. I. B.","doi":"10.21161/mjm.230196","DOIUrl":"https://doi.org/10.21161/mjm.230196","url":null,"abstract":"","PeriodicalId":18178,"journal":{"name":"Malaysian Journal of Microbiology","volume":"11 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135170452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Genotypic identification of hypervirulent Klebsiella pneumoniae in Anbar, Iraq","authors":"Al-Fahdawi, A. M., Mutter, T. Y., Zylstra, G. J.","doi":"10.21161/mjm.230269","DOIUrl":"https://doi.org/10.21161/mjm.230269","url":null,"abstract":"","PeriodicalId":18178,"journal":{"name":"Malaysian Journal of Microbiology","volume":"139 1","pages":""},"PeriodicalIF":0.3,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80900414","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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