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PRODUCTION OF EXOPOLYSACCHARIDE FROM LOCAL ISOLATES OF Rhizobium leguminosarum biovar viciae 豆科根瘤菌本地分离株的胞外多糖生产
Pub Date : 2022-01-01 DOI: 10.33899/magrj.2022.134702.1184
Wjdan Al-Obaidy
Fourteen local isolates of Rhizobium leguminosarum biovar viciae were isolated from root nodules of Vicia faba plants collected from agricultural soils of different regions of Nineveh Governorate/Iraq. Six isolates with mucoid colonies were selected as follows: WS14, WS15, WS18, WS21., WS23 and WS26. These local isolates were incubated for periods 1, 2, 3 and 4 days. Dry biomass weight and exopolysaccharide (EPS) (g/L) were recorded. Results revealed that WS18 isolate was the best among the six mentioned isolates of R. leguminosarum biovar viciae , which it gave the maximum production of EPS (6.31 g/L) after two days of incubation. Maximum dry biomass w1.22 g/L after three days of incubation by the same isolate. The effect of addition of different carbon sources to yeast extract mannitol (YEM) broth medium on EPS production by WS18 isolate showed that mannitol, as carbon source was the best for production of EPS (6.36 g/L), after two days of incubation. Two percent was the optimal concentration of mannitol which supported the maximum production of EPS (9.67 g/L) after two days of incubation. The effect of addition of different nitrogen sources at 0.10 % concentration to YEM broth medium (supplement with 2.0 % mannitol) revealed that NaNO 3 gave the best production which reached to 12.94 g/L after two days of incubation. The effective concentration of NaNO 3 support the maximum production was 0.1 %.
从伊拉克尼尼微省不同地区农业土壤中收集的蚕豆根瘤中分离出14株豆科根瘤菌。筛选出6株具有黏液菌落的分离株:WS14、WS15、WS18、WS21。, WS23和WS26。这些本地分离株孵育1、2、3和4天。记录干生物量和胞外多糖(EPS) (g/L)。结果表明,在6株豆科致病菌中,WS18菌株的EPS产量最高,培养2 d后EPS产量达到6.31 g/L。同一菌株孵育3天后的最大干生物量为1.22 g/L。酵母浸膏甘露醇(YEM)肉汤培养基中添加不同碳源对WS18分离菌EPS产量的影响表明,培养2 d后,甘露醇作为碳源对EPS产量的影响最大(6.36 g/L)。当甘露醇浓度为2%时,培养2天后EPS产量最高(9.67 g/L)。在YEM肉汤培养基中添加浓度为0.10%的不同氮源(添加2.0%甘露醇)的效果表明,培养2天后,NaNO 3的产量最高,达到12.94 g/L。纳米3的有效支持浓度为0.1%,产率最高。
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引用次数: 0
INFLUENCE OF FOLIAR APPLICATION OF ATONIK ON VEGETATIVE GROWTH AND YIELD OF TWO PEA (Pisum sativum L.) CULTIVARS 叶面施药对两种豌豆营养生长和产量的影响品种
Pub Date : 2022-01-01 DOI: 10.33899/magrj.2022.133920.1173
D. Zrar, B. Aziz, Sawsan Ali
Article The study was carried out at Grdarasha field in Erbil from November 4 th of 2019 to April 20 th , 2020.The purpose of this study was to study different levels of foliar spray of Atonik (0, 0.2, 0.4, 0.6, 0.8, and 1 ml.L -1 ) affected the growth and yield of two pea cultivars (Utrillo and Nihal). The majority of the parameters investigated yielded significant outcomes. Cultivars had a substantial reaction on some of the tests, according to the findings. Parameters of pea vegetative growth and yield, the Utrillo cultivar produced the best results. Furthermore, Atonik foliar spraying had a considerable impact. The number of leaves and branches has the biggest influence on vegetative parameters (110.74 and 2.69 respectively), according to the findings (1ml.L -1 ). From 0.2 ml.L -1 of Atonik the maximum values of vegetative growth, fresh weight, and total chlorophyll were recorded. Atonik foliar spraying also improved yield parameters significantly. However, at 0.2 ml.L - 1 of Atonik, the highest numbers of seeds per pod, yield per plant, yield per plot, and yield per hectare (6.00, 0.16 kg, 1.00 kg, and 1.68 tons, respectively) were obtained. Meanwhile, the majority of vegetative development and yield indices, particularly Nihal, exhibited a substantial response to Atonik treatment.
研究于2019年11月4日至2020年4月20日在埃尔比勒Grdarasha油田进行。本试验旨在研究不同喷施浓度(0、0.2、0.4、0.6、0.8和1 ml.L -1)对2个豌豆品种(乌特里洛和尼哈尔)生长和产量的影响。调查的大多数参数产生了显著的结果。根据研究结果,品种在一些测试中有很大的反应。在豌豆营养生长和产量参数方面,尤特里洛品种的效果最好。此外,叶面喷施有相当大的影响。叶和枝的数量对营养参数的影响最大(分别为110.74和2.69)。L -1)。从0.2 ml.L -1的浓度开始,记录了植株的营养生长、鲜重和总叶绿素的最大值。叶面喷施也显著提高了产量参数。而在0.2 ml.L - 1浓度下,每荚种子数、单株产量、每地块产量和每公顷产量最高(分别为6.00、0.16 kg、1.00 kg和1.68吨)。与此同时,大多数营养发育和产量指标,特别是Nihal,对Atonik处理表现出明显的响应。
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引用次数: 0
AN INVESTIGATION INTO THE EFFECT OF USING DIFFERENT HARVESTING METHODS ON THE INDICATORS OF POTATO CROP LOSS 不同收获方式对马铃薯作物损失指标影响的研究
Pub Date : 2022-01-01 DOI: 10.33899/magrj.2022.133350.1168
Arkan M. A. Sedeeq, Othman M.M. Tofeq, Saleh Al-Slevani
An experiment was conducted in Nineveh, Iraq. The study evaluates the efficacy of three methods for harvesting potato crops (Semi-Mechanized Harvesting, which includes single-row and two-row harvesting, and manual harvesting). Evaluation through study effects of ways on the undamaged tubers, severely damaged tubers, slightly damaged tubers, qualitative loss, quantitative loss, produced tubers, the time of the harvest process, and harvest costs with the total loss which goes in line with each method). The best performance was obtained for semi-mechanized harvest (2-row harvesters); it recorded the lowest percentage of severely damaged tubers of 0.2 ton. ha -1 and the highest rate of lifted tubers was 24.6 ton. ha-1. The production of the best sample about 25.49 ton. ha -1 while the highest productivity about 2.24 ha. h -1 . Moreover, the lowest harvest costs were 4.5$. ha -1 , and less time for completion of the harvest process was 0.44 h. ha -1 . At the same time, the manual harvesting process has recorded a higher percentage of undamaged tubers was 20.76 ton. ha-1 and a lower percentage of damaged tubers about 3.63 ton. ha -1 , a lower percentage of quantitative loss of 0.49 ton. ha-1, and less loss in total quantity of 877 $. ha -1 . Finally, in the manual harvesting process, the farmer needs 45 workers to complete per hectare within an hour. College of Agriculture and Forestry, University of Mosul.
在伊拉克尼尼微进行了一项实验。本研究评估了三种马铃薯作物收获方法(半机械化收获,包括单行和两行收获,以及人工收获)的效果。通过研究不同方法对未损块茎、重度损块茎、轻度损块茎、质损块茎、量损块茎、产块茎、采收过程时间、采收成本的影响(与每种方法的总损失相一致)进行评价。半机械化(二行收割机)收获效果最好;严重受损块茎的比例最低,为0.2吨。Ha -1,块茎举升率最高,达24.6吨。农业的。生产最好的样品约25.49吨。Ha -1,最高产量约2.24 Ha。H -1。此外,最低的收获成本为4.5美元。Ha -1,完成收获过程的时间缩短了0.44 h. Ha -1。与此同时,人工采收过程中未受损块茎的比例较高,为20.76吨。Ha-1和较低的块茎损坏率约为3.63吨。Ha -1的数量损失率较低,为0.49吨。Ha-1,总金额877美元损失少。Ha -1。最后,在人工收割过程中,农民需要45名工人在一小时内完成每公顷。摩苏尔大学农林学院。
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引用次数: 0
PHYSIOLOGICAL ASPECTS OF PHYTOCHEMICALS AS ANTIOXIDANTS ON POULTRY: (Article Review) 植物化学物质作为抗氧化剂对家禽的生理作用:(文章综述)
Pub Date : 2022-01-01 DOI: 10.33899/magrj.2022.135167.1193
A. Abdul-majeed, Ghadeer A. Rahawi, S. Abdul–Rahman
Antioxidants have an important and vital function in preserving animal health and have a special role in animal physiological, reproductive, and productive performance. In general, poultry are subjected to a wide and varied range of stressors, including environmental, biological, nutritional, and productive stresses. These stresses frequently occur in quick and intensive poultry production systems, causing health issues related to their physiological and productive performance that may increase the formation of free radicals, which in turn cause lipid peroxidation, leading to a decline in their physiological and productive performance. Therefore, it requires an antioxidant system that can delay the initiation or slow the going rate of harmful oxidative reactions and prevent cellular and molecular damage that results from the influence of free radicals in the cellular metabolism process. Phytochemicals, which are plant-derived non-vitamin, non-mineral substances such as flavonoids and carotenoids, are considered dietary nonenzymatic antioxidants that play a protective role against oxidative reactions and minimize the effects of the body's oxidative stress, which supports the animal's physiological productive efficiency and health.
抗氧化剂在保护动物健康方面具有重要的作用,在动物的生理、繁殖和生产性能方面具有特殊的作用。一般来说,家禽受到各种各样的压力,包括环境、生物、营养和生产压力。这些压力经常发生在快速和集约化的家禽生产系统中,导致与它们的生理和生产性能相关的健康问题,这可能会增加自由基的形成,进而导致脂质过氧化,导致它们的生理和生产性能下降。因此,它需要一个抗氧化系统,可以延缓或减缓有害氧化反应的开始或进行速度,防止细胞代谢过程中自由基的影响造成细胞和分子的损伤。植物化学物质是植物衍生的非维生素、非矿物质物质,如类黄酮和类胡萝卜素,被认为是饮食中的非酶抗氧化剂,具有防止氧化反应的保护作用,并最大限度地减少身体氧化应激的影响,从而支持动物的生理生产效率和健康。
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引用次数: 0
MOLECULAR DETECTION OF RAW MEAT FOR SOME ANIMAL SPECIES USING RFLP-PCR TECHNIQUE 利用rflp-pcr技术对部分动物生肉进行分子检测
Pub Date : 2022-01-01 DOI: 10.33899/magrj.2022.132675.1156
K. Taha
Mitochondrial cytochrome b gene plays a serious role in studying adulteration of meat species. This research study designed to distinguish the raw meat species of sheep, goat, cattle and donkey using RFLP-PCR technique of a universal cyt b gene 359bp. Ten indigenous samples were collected from each animal in different parts of the body. All the samples were processed for DNA isolation and amplified with a Polymerase chain reaction (PCR). The amplicons were cleavage with HinfI and RsaI restriction enzymes, digestion of PCR product resulted in production of specific characterization bands for each species then analyzed by agarose electrophoresis. HinfI RE created three fragments for sheep, goat and cattle, with some similarities in a few bands between them, while yielded two bands for donkey. RsaI RE produced two bands for all species with different length except sheep and goats have the same length. Thus, results recommend that the RFLP-PCR technique with HinfI and RsaI play an important role to detect the animal meat species, since it is a fast, simple and easily handle method for identification of animal species.
线粒体细胞色素b基因在肉类掺假研究中起着重要作用。本研究采用通用型cyt b基因359bp的RFLP-PCR技术对绵羊、山羊、牛和驴的生肉品种进行了鉴别。从每只动物身体的不同部位收集了10个本地样本。所有样品均进行DNA分离处理,并采用聚合酶链反应(PCR)扩增。用HinfI酶和RsaI酶切扩增子,酶切产物得到每个物种的特异特征带,然后琼脂糖电泳分析。hini RE为绵羊、山羊和牛创造了三个片段,它们之间有一些相似之处,而为驴创造了两个片段。除了绵羊和山羊长度相同外,RsaI RE在所有不同长度的物种中均产生两条条带。因此,HinfI和RsaI结合的RFLP-PCR技术是一种快速、简便、易操作的动物物种鉴定方法,在动物肉种检测中具有重要的应用价值。
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引用次数: 0
EFFECT OF SPRAYING WITH SOME AGRICULTURAL CHEMICALS ON INDUCING TOMATO PLANT RESISTANCE AGAINST TETRANYCHUS URTICAE (KOCH) 农药喷施对番茄抗荨麻疹叶螨的影响
Pub Date : 2021-12-12 DOI: 10.33899/magrj.2021.131985.1147
Abdul Aziz Mustafa, Nabil Al Mallah
Article information Article history: Received: 31/10/2021 Accepted: 12/12/2021 Available: 12/31/2021
文章信息文章历史:收稿日期:31/10/2021收稿日期:12/12/2021收稿日期:12/31/2021
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引用次数: 0
THE RESPONSE OF SOME FORAGE GRASS CROPS TO NITROGEN FERTILIZER 几种牧草作物对氮肥的响应
Pub Date : 2021-12-07 DOI: 10.33899/magrj.2021.130762.1135
Salim AL- Gazhal
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引用次数: 0
The effect of using Coenzyme Q10 and wheat germ oil on some blood characteristics of stressed rabbits 使用辅酶Q10和小麦胚芽油对应激家兔某些血液特征的影响
Pub Date : 2021-11-17 DOI: 10.33899/magrj.2021.131859.1142
Eman A. M. Al-Samarai, Abduljaleel Al-Janabi
* Part of M.Sc. thesis submitted by the first author To determining effect of Coenzyme Q10 (CoQ10)and wheat germ oil(WGO) on hematological , and blood biochemical treats in rabbits buck exposed to oxidative stress, 54 animal aged 4-5 month randomly distributed into six group. Nine animals / group. as following: the first was a productive ration + tap water, the 2nd group consumption normal diet + Water with 0.04%H2O2, G3 and G5: supplemented CoQ10 100 mg/kg fodder without and with H2O2, respectively. Moreover, the G4 and G6: supplied 3 gm. WGO /kg of feed without and with H2O2 in drinking water, respectively. The results showed: No significant difference (P≤0.05) among group in the (RBC), G3 recorded a significant increase in the PCV, corresponding to a significant decrease in the MCV. The sixth group recorded increasing in the hemoglobin concentration, which led to a significant increase in the MCHC. The second treatment recorded decrease total protein, and globulin, Regarding the lipid profile, there was a significant increase in the level of triglycerides and cholesterol with H2O2. There was a significant decrease in ALT and AST levels for the third and fourth treatments compared with the rest of the oxidative stress treatments. With a significant decrease in glucose concentration, creatinine, urea and cortisol levels. College of Agriculture and Forestry, University of Mosul. This is an open access article under the CC BY 4.0 license (https://magrj.mosuljournals.com/ ).
为了研究辅酶Q10 (CoQ10)和小麦胚芽油(WGO)对氧化应激家兔血液学和血液生化的影响,将54只4 ~ 5月龄家兔随机分为6组。9只动物/组。结果如下:第一组为生产日粮+自来水,第二组为正常日粮+ 0.04%H2O2水,G3和G5分别添加辅酶q10 100 mg/kg不添加H2O2和添加H2O2饲料。此外,G4和G6分别在饮用水中添加H2O2和不添加H2O2的饲料中添加3 gm. WGO /kg。结果显示:各组红细胞(RBC)差异无统计学意义(P≤0.05),G3组PCV显著升高,对应MCV显著降低。第六组血红蛋白浓度升高,导致MCHC显著升高。第二次处理记录了总蛋白和球蛋白的减少,关于脂质谱,过氧化氢显著增加了甘油三酯和胆固醇的水平。与其他氧化应激处理相比,第三和第四处理的ALT和AST水平显著降低。葡萄糖浓度、肌酐、尿素和皮质醇水平显著降低。摩苏尔大学农林学院。这是一篇基于CC BY 4.0许可(https://magrj.mosuljournals.com/)的开放获取文章。
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引用次数: 0
THE PRODUCTION EFFICIENCY AND DETERMINANTS OF THE CHICKPEA CROP IN NINEVEH GOVERNORATE FOR THE PRODUCTION SEASON 2019 尼尼微省2019生产季鹰嘴豆作物的生产效率和决定因素
Pub Date : 2021-11-14 DOI: 10.33899/magrj.2021.132054.1146
mhasin Al-jebory
Article information Article history: Received:31/10/2021 Accepted:14/11/2021 Available: 12/31/2021
文章信息文章历史:收稿日期:31/10/2021收稿日期:14/11/2021收稿日期:12/31/2021
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引用次数: 0
STYDY HETEROSIS IN MAIZE 玉米柱头杂种优势
Pub Date : 2021-11-08 DOI: 10.33899/magrj.2021.130249.1132
M. Altaweel, Wiam Yahya Rasheed Al-Shakarchy Asst.Prof
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引用次数: 0
期刊
Mesopotamia Journal of Agriculture
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