Pub Date : 2018-08-30DOI: 10.3329/aajbb.v3i2.64819
N. Ahmed, K. Ahamed, M. S. Rahman
The experiment was conducted in the experimental field of Sher-e-Bangla Agricultural University, Dhaka-1207 to find out the yield and yield contributing attributes of rice varieties under different planting times. The experiment comprised of two factors- factor a: Planting time (2): T1: 24th January planting; T2: 23th February planting and factor b: Rice variety (5): V1: BRRI dhan29; V2: BRRI hybrid 2; V3: Hera 2; V4: Tia and V5: Taj 1. The experiment was laid out in a randomized complete block design (RCBD) with three replications. Among the different planting time and varieties, 24th January planting and Hera 2 were found superior in terms of yield and yield contributing attributes of rice varieties. Irrespective of planting times, the highest panicle length (24.64 cm), number of effective tillers hill-1(13.33), filled grains panicle-1 (83.17), 1000-grain weight (25.14 g), grain yield (4.30 t ha-1), straw yield (5.21 t ha-1), biological yield (9.43 t ha-1) and harvest index (45.89%) were achieved. However, Hera 2 provided the highest grain yield (4.64 t ha-1) at 24th January planting compared to other combination. It meant that Hera 2 performed well with 24th January planting. Asian Australas. J. Biosci. Biotechnol. 2018, 3 (2), 162-169
本试验在达卡Sher-e-Bangla农业大学(Sher-e-Bangla Agricultural University, Dhaka-1207)试验田进行,旨在了解不同种植期下水稻品种的产量及产量贡献属性。试验由两个因素组成:因素a:种植时间(2):T1: 1月24日种植;T2: 2月23日种植和因子b:水稻品种(5):V1: BRRI dhan29;V2: BRRI杂交2;V3:赫拉2;V4: Tia和V5: Taj 1。试验采用随机完全区组设计(RCBD), 3个重复。在不同的种植时间和品种中,1月24日种植和赫拉2号在产量和产量贡献属性方面都具有优势。在不同种植时间下,水稻穗长(24.64 cm)、有效分蘖数(13.33)、实粒数(83.17)、千粒重(25.14 g)、籽粒产量(4.30 t ha-1)、秸秆产量(5.21 t ha-1)、生物产量(9.43 t ha-1)和收获指数(45.89%)均达到最高。但在1月24日播种时,与其他组合相比,赫拉2的产量最高(4.64 t hm -1)。这意味着赫拉2号在1月24日播种时表现良好。亚洲Australas。j . Biosci。生物技术,2018,3 (2),162-169
{"title":"Yield and yield contributing attributes of rice (Oryza Sativa L.) under different planting dates in boro season","authors":"N. Ahmed, K. Ahamed, M. S. Rahman","doi":"10.3329/aajbb.v3i2.64819","DOIUrl":"https://doi.org/10.3329/aajbb.v3i2.64819","url":null,"abstract":"The experiment was conducted in the experimental field of Sher-e-Bangla Agricultural University, Dhaka-1207 to find out the yield and yield contributing attributes of rice varieties under different planting times. The experiment comprised of two factors- factor a: Planting time (2): T1: 24th January planting; T2: 23th February planting and factor b: Rice variety (5): V1: BRRI dhan29; V2: BRRI hybrid 2; V3: Hera 2; V4: Tia and V5: Taj 1. The experiment was laid out in a randomized complete block design (RCBD) with three replications. Among the different planting time and varieties, 24th January planting and Hera 2 were found superior in terms of yield and yield contributing attributes of rice varieties. Irrespective of planting times, the highest panicle length (24.64 cm), number of effective tillers hill-1(13.33), filled grains panicle-1 (83.17), 1000-grain weight (25.14 g), grain yield (4.30 t ha-1), straw yield (5.21 t ha-1), biological yield (9.43 t ha-1) and harvest index (45.89%) were achieved. However, Hera 2 provided the highest grain yield (4.64 t ha-1) at 24th January planting compared to other combination. It meant that Hera 2 performed well with 24th January planting. \u0000Asian Australas. J. Biosci. Biotechnol. 2018, 3 (2), 162-169","PeriodicalId":184754,"journal":{"name":"Asian-Australasian Journal of Bioscience and Biotechnology","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129744634","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-04-28DOI: 10.3329/aajbb.v3i1.64758
Sabiha Sultana Soma, K. Nazir, M. T. Rahman, Md. Mizanur Rahman, M. Ara, R. Sultana, M. R. Haydar, M. Siddique, M. Rahman
The present study was carried out for the isolation and molecular detection of duck plague virus (DPV) for the development of inactivated vaccine seed from the local outbreaks. A total of 12 suspected dead duck samples were collected from commercial farms and local market at Sunamganj, Netrokona and Mymensingh districts. Then, the samples were processed and prepared inocula were inoculated into 9-12 days old duck embryonated eggs. In duck embryonated eggs, several passages (3-4) were performed before infection into DEF cell culture. Presence of viral DNA was confirmed by PCR using the primer for DNA polymerase gene. After PCR confirmation, virus cultured in DEF cell was used for the preparation of formalin (0.12%) inactivated and oil based adjuvanted vaccine and was experimentally injected to 18 ducklings and 5 were kept as control. TCID50 of the selected virus for vaccine preparation was 108.70/ml. The mean passive haemagglutination assay (PHA) titre of sera of samples at 0 days, 7 days and 14 days post vaccination were 4.0±0, 14.22±1.78 and 44.44±4.4, respectively, which indicated significant (p<0.01) increase of antibody titre. Embryonated duck eggs and DEF cell culture are effective for virus isolation and on the basis of PHA test, it could also be suggested that the experimentally developed DP vaccine can be used successfully for the prevention of DP in Bangladesh. Asian Australas. J. Biosci. Biotechnol. 2018, 3 (1), 78-85
{"title":"Isolation and molecular detection of duck plague virus for the development of vaccine seed","authors":"Sabiha Sultana Soma, K. Nazir, M. T. Rahman, Md. Mizanur Rahman, M. Ara, R. Sultana, M. R. Haydar, M. Siddique, M. Rahman","doi":"10.3329/aajbb.v3i1.64758","DOIUrl":"https://doi.org/10.3329/aajbb.v3i1.64758","url":null,"abstract":"The present study was carried out for the isolation and molecular detection of duck plague virus (DPV) for the development of inactivated vaccine seed from the local outbreaks. A total of 12 suspected dead duck samples were collected from commercial farms and local market at Sunamganj, Netrokona and Mymensingh districts. Then, the samples were processed and prepared inocula were inoculated into 9-12 days old duck embryonated eggs. In duck embryonated eggs, several passages (3-4) were performed before infection into DEF cell culture. Presence of viral DNA was confirmed by PCR using the primer for DNA polymerase gene. After PCR confirmation, virus cultured in DEF cell was used for the preparation of formalin (0.12%) inactivated and oil based adjuvanted vaccine and was experimentally injected to 18 ducklings and 5 were kept as control. TCID50 of the selected virus for vaccine preparation was 108.70/ml. The mean passive haemagglutination assay (PHA) titre of sera of samples at 0 days, 7 days and 14 days post vaccination were 4.0±0, 14.22±1.78 and 44.44±4.4, respectively, which indicated significant (p<0.01) increase of antibody titre. Embryonated duck eggs and DEF cell culture are effective for virus isolation and on the basis of PHA test, it could also be suggested that the experimentally developed DP vaccine can be used successfully for the prevention of DP in Bangladesh. \u0000Asian Australas. J. Biosci. Biotechnol. 2018, 3 (1), 78-85","PeriodicalId":184754,"journal":{"name":"Asian-Australasian Journal of Bioscience and Biotechnology","volume":"22 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125172290","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-04-28DOI: 10.3329/aajbb.v3i1.64755
Debobrota Roy Goswami, R. Islam, M. Hasan, S. Sarkar, F. Aziz, Mst. Misrat Masuma Pervez, M. Salauddin
The current study was designed to improve milk production and milk fat percentage by supplemented with black cumin and domperidone for thirteen weeks during lactation in indigenous cows. The experiment was performed in Kaligonjupazila of Lalmonirhat under the department of Physiology and Pharmacology, HSTU, Dinajpur, Bangladesh. At 7 days of lactation 16 deshi cows were randomly divided into 4 groups (n=4 lactating cows in each group). Group T1 was kept as control, Group T2 was treated with domperidone (Motigut®) orally at a dose of 100 mg /day per cow, Group T3 was treated with Black Cumin Seed Powder (BCSP) at a dose of 80 g orally per cow per day and Group T4 was treated with combination of domperidone with Black Cumin Seed Powder (BCSP) as above mentioned doses. All the animals were dewormed and vaccinated at initial stage. Over the course of the trial, observations were recorded for milk production and fat percentage in milk. Milk production were increased significantly (P<0.05) in all treated groups compared to the control group and highest was recorded in combined domperidone with Black Cumin Seed Powder (BCSP) group (Group T4). Production level increased significantly (p<0.05) in all treated groups and highest was recorded in cows of group T4. The present study reveals that additional supplementation with domperidone plus Black Cumin Seed Powder (BCSP) in feed of lactating cows should better performance over other groups in respect to increase milk production and fat percentage in milk without any health hazard in indigenous cows. This technology may be transferred to farmers since drug (Domperidone) and supplement (BCSP) is available and require minimum stillness. Asian Australas. J. Biosci. Biotechnol. 2018, 3 (1), 67-71
本试验旨在提高奶牛泌乳期13周内黑孜然和多潘立酮的产奶量和乳脂率。实验在孟加拉国迪纳杰布尔HSTU生理学和药理学系的Lalmonirhat的Kaligonjupazila进行。在泌乳第7天,将16头德始奶牛随机分为4组(每组n=4头泌乳奶牛)。T1组作为对照组,T2组口服多潘立酮(Motigut®),剂量为100 mg /d /头奶牛,T3组口服黑孜然籽粉(BCSP),剂量为80 g /d /头奶牛,T4组采用多潘立酮与黑孜然籽粉(BCSP)联合治疗,剂量均为上述剂量。所有动物在初始阶段都进行了驱虫和疫苗接种。在试验过程中,记录了乳产量和乳中脂肪百分比的观察结果。各处理组产奶量均显著高于对照组(P<0.05),其中以多潘立酮与黑孜然子粉(BCSP)联合组(T4组)最高。各处理组奶牛的生产水平均显著提高(p<0.05),以T4组最高。本研究表明,在本地奶牛饲料中添加多潘立酮和黑孜然种子粉(BCSP),在提高产奶量和牛奶脂肪率方面优于其他组,且不会对奶牛健康造成危害。这种技术可以转让给农民,因为有药物(多潘立酮)和补充剂(BCSP)可用,并且需要最小的静止。亚洲Australas。j . Biosci。生物技术,2018,3 (1),67-71
{"title":"Comparative efficacy of black cumin and domperidone therapy on milk yield and fat percentage of indigenous cow","authors":"Debobrota Roy Goswami, R. Islam, M. Hasan, S. Sarkar, F. Aziz, Mst. Misrat Masuma Pervez, M. Salauddin","doi":"10.3329/aajbb.v3i1.64755","DOIUrl":"https://doi.org/10.3329/aajbb.v3i1.64755","url":null,"abstract":"The current study was designed to improve milk production and milk fat percentage by supplemented with black cumin and domperidone for thirteen weeks during lactation in indigenous cows. The experiment was performed in Kaligonjupazila of Lalmonirhat under the department of Physiology and Pharmacology, HSTU, Dinajpur, Bangladesh. At 7 days of lactation 16 deshi cows were randomly divided into 4 groups (n=4 lactating cows in each group). Group T1 was kept as control, Group T2 was treated with domperidone (Motigut®) orally at a dose of 100 mg /day per cow, Group T3 was treated with Black Cumin Seed Powder (BCSP) at a dose of 80 g orally per cow per day and Group T4 was treated with combination of domperidone with Black Cumin Seed Powder (BCSP) as above mentioned doses. All the animals were dewormed and vaccinated at initial stage. Over the course of the trial, observations were recorded for milk production and fat percentage in milk. Milk production were increased significantly (P<0.05) in all treated groups compared to the control group and highest was recorded in combined domperidone with Black Cumin Seed Powder (BCSP) group (Group T4). Production level increased significantly (p<0.05) in all treated groups and highest was recorded in cows of group T4. The present study reveals that additional supplementation with domperidone plus Black Cumin Seed Powder (BCSP) in feed of lactating cows should better performance over other groups in respect to increase milk production and fat percentage in milk without any health hazard in indigenous cows. This technology may be transferred to farmers since drug (Domperidone) and supplement (BCSP) is available and require minimum stillness. \u0000Asian Australas. J. Biosci. Biotechnol. 2018, 3 (1), 67-71","PeriodicalId":184754,"journal":{"name":"Asian-Australasian Journal of Bioscience and Biotechnology","volume":"29 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128796272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-04-28DOI: 10.3329/aajbb.v3i1.64745
MM Ali, J. Alam, S. Islam, A. Rahman, Shabbir Ahmed, H. Zaman
This study was conducted to find out the comparative growth performance of six color mutants Japanese quail in Bangladesh. Under this experiment the mortality had shown no significant differences among different isolated plumage color mutants (p>0.05). At day- old it is clear that day- old weight was superior in BB- Black than other mutants (p<0.05). At 1st week the body weight was higher in BB- Black, intermediate in BB-Dhakaya and BB- Tuxedo and the lowest in BB- White, BB- Fawn and BB- Rosseta (p<0.05). At 2nd and 5th weeks of age no significant differences in body weight was found among 6 different isolated plumage color mutants (p>0.05). At 3rd week the body weight is higher in BB- Rosseta, intermediate in BB- White, BB- Fawn, BB- Dhakaya and BB- Black and the lowest in BB- Tuxedo (p<0.05). At 4th week the body weight was higher in BB- Black and BB- Fawn, intermediate in BB- Rosseta, BB- White and BB- Dhakaya and the lowest in BB- Tuxedo (p<0.05). The body weight at marketing age (6 weeks) was higher in BB- Black than other mutants whereas other mutants had shown lower values except BB- Fawn (p<0.05). So BB-Black mutants quail is the best one for farming of quail in perspective of growth in Bangladesh. Asian Australas. J. Biosci. Biotechnol. 2018, 3 (1), 7-10
{"title":"Comparative study on growth of different mutants Japanese quail in Bangladesh","authors":"MM Ali, J. Alam, S. Islam, A. Rahman, Shabbir Ahmed, H. Zaman","doi":"10.3329/aajbb.v3i1.64745","DOIUrl":"https://doi.org/10.3329/aajbb.v3i1.64745","url":null,"abstract":"This study was conducted to find out the comparative growth performance of six color mutants Japanese quail in Bangladesh. Under this experiment the mortality had shown no significant differences among different isolated plumage color mutants (p>0.05). At day- old it is clear that day- old weight was superior in BB- Black than other mutants (p<0.05). At 1st week the body weight was higher in BB- Black, intermediate in BB-Dhakaya and BB- Tuxedo and the lowest in BB- White, BB- Fawn and BB- Rosseta (p<0.05). At 2nd and 5th weeks of age no significant differences in body weight was found among 6 different isolated plumage color mutants (p>0.05). At 3rd week the body weight is higher in BB- Rosseta, intermediate in BB- White, BB- Fawn, BB- Dhakaya and BB- Black and the lowest in BB- Tuxedo (p<0.05). At 4th week the body weight was higher in BB- Black and BB- Fawn, intermediate in BB- Rosseta, BB- White and BB- Dhakaya and the lowest in BB- Tuxedo (p<0.05). The body weight at marketing age (6 weeks) was higher in BB- Black than other mutants whereas other mutants had shown lower values except BB- Fawn (p<0.05). So BB-Black mutants quail is the best one for farming of quail in perspective of growth in Bangladesh. \u0000Asian Australas. J. Biosci. Biotechnol. 2018, 3 (1), 7-10","PeriodicalId":184754,"journal":{"name":"Asian-Australasian Journal of Bioscience and Biotechnology","volume":"159 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131935112","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-04-28DOI: 10.3329/aajbb.v3i1.64756
M. Begum, R. Islam, M. Hasan, S. Sarkar, F. Aziz, Misrat Masuma Pervez, Debobrota Roy Goswami
This study was carried out in the Department of Physiology and Pharmacology at Hajee Mohammad Danesh Science and Technology University to evaluate the effect of ipil ipil and calcium supplementation on egg production and live body weight of Japanese quail. Forty two days old “Japanese Quail” were divided into four groups (n=10 birds in each group). Group T0 was considered as control, fed only with commercial layer ration. Group T1 was additionally supplemented with 2 g grinded ipil ipil leaves, Group T2 with 2 g Dietary Ca sources and Group T3with 2 g grinded ipil ipil leaves with 2 g dietary Ca sources per kg feed. Observations were recorded for growth performance, egg production and egg quality of Japanese quail. Body weight (g) was increased significantly (p<0.05) in all treated groups in compared to control and best gain was recorded in combined action of ipil ipil and dietary Ca sources supplemented groups (155.6±2.59). In all treatment groups there were significantly increased in egg production, compare to control group. Best result was found in combination of ipil ipil and dietary calcium supplementation. The present study reveals that combine supplementation of ipil ipil and dietary Ca sources gives better effects over other groups in respect to growth performance, egg production and egg quality without any health hazard of Japanese quail. Asian Australas. J. Biosci. Biotechnol. 2018, 3 (1), 72-77
本研究在Hajee Mohammad Danesh科技大学生理与药理学系进行,旨在评估ipil和补钙对日本鹌鹑产蛋量和活重的影响。42日龄“日本鹌鹑”分为4组,每组10只。T0组为对照,仅饲喂商品蛋鸡日粮。T1组在每kg饲料中添加2 g油梨叶粉,T2组添加2 g饲粮钙源,t3组添加2 g油梨叶粉,饲粮钙源为2 g。对日本鹌鹑的生长性能、产蛋量和蛋品质进行了观察。与对照组相比,各处理组的体重(g)均显著增加(p<0.05),其中ipil与饲粮钙源补充组的增重最高(155.6±2.59)。与对照组相比,所有处理组的产蛋量均显著增加。ipil与膳食补钙联合使用效果最好。本研究表明,在日本鹌鹑的生长性能、产蛋量和蛋品质方面,ipil和饲粮钙源的组合添加效果优于其他各组,且无健康危害。亚洲Australas。j . Biosci。生物技术,2018,3 (1),72-77
{"title":"Effects of ipil ipil and calcium dietary sources on egg and growth performances of Japanese quail","authors":"M. Begum, R. Islam, M. Hasan, S. Sarkar, F. Aziz, Misrat Masuma Pervez, Debobrota Roy Goswami","doi":"10.3329/aajbb.v3i1.64756","DOIUrl":"https://doi.org/10.3329/aajbb.v3i1.64756","url":null,"abstract":"This study was carried out in the Department of Physiology and Pharmacology at Hajee Mohammad Danesh Science and Technology University to evaluate the effect of ipil ipil and calcium supplementation on egg production and live body weight of Japanese quail. Forty two days old “Japanese Quail” were divided into four groups (n=10 birds in each group). Group T0 was considered as control, fed only with commercial layer ration. Group T1 was additionally supplemented with 2 g grinded ipil ipil leaves, Group T2 with 2 g Dietary Ca sources and Group T3with 2 g grinded ipil ipil leaves with 2 g dietary Ca sources per kg feed. Observations were recorded for growth performance, egg production and egg quality of Japanese quail. Body weight (g) was increased significantly (p<0.05) in all treated groups in compared to control and best gain was recorded in combined action of ipil ipil and dietary Ca sources supplemented groups (155.6±2.59). In all treatment groups there were significantly increased in egg production, compare to control group. Best result was found in combination of ipil ipil and dietary calcium supplementation. The present study reveals that combine supplementation of ipil ipil and dietary Ca sources gives better effects over other groups in respect to growth performance, egg production and egg quality without any health hazard of Japanese quail. \u0000Asian Australas. J. Biosci. Biotechnol. 2018, 3 (1), 72-77","PeriodicalId":184754,"journal":{"name":"Asian-Australasian Journal of Bioscience and Biotechnology","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114266305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-04-28DOI: 10.3329/aajbb.v3i1.64747
M. Islam, M. Sabrin, Md Kabir, S. Karim, Tahmina Sikder
The present study was performed to investigate the prevalence of multidrug resistant (MDR) food-borne pathogens in raw chicken meat in Dhaka city, Bangladesh: an increasing food safety concern. A total of 100 meat samples (05 from each market) were collected from randomly selected 20 retail markets of Dhaka city using sterile polythene bags in a view to prevent extraneous contamination. The collected meat samples were then processed and inoculated onto nutrient broth and nutrient agar plates for isolation. The isolated organisms were identified based on staining, motility, cultural and biochemical properties according to standard laboratory methods. The isolated bacteria were also subjected to characterize their antibiotic sensitivity. In the present study, it was revealed that 100% of samples were contaminated by at least one species of bacteria belonging to 5 genera such as Staphylococcus, Escherichia coli, Salmonella, Enterobacter and Bacillus. Out of total 100 meat samples, 56% were contaminated with Escherichia coli whereas 42% were contaminated with Coagulase positive Staphylococcus and 36% were Salmonella species followed by 20%, 14% and 10% were contaminated with Enterobacter species, Coagulase negative Staphylococcus and Bacillus species respectively. A total of 174 bacteria were isolated and identified from raw chicken meat samples inspected of which 29.89% were Escherichia coli, 24.14% were Coagulase positive Staphylococcus, 20.69% were Salmonella species, 11.49% were Enterobacter species, 8.05% were Coagulase negative Staphylococcus and 5.75% were Bacillus species. The antimicrobial sensitivity tests showed that 96.15% of Escherichia coli (50 out of 52), 95.24% of Coagulase positive Staphylococcus (40 out of 42) and 86.11% of Salmonella (31 out of 36) isolates displayed multidrug resistance phenotypes (resistant to more than two antimicrobial agents). All most all the isolates of E. coli, coagulase positive Staphylococcus and Salmonella were more resistant to tetracycline, amoxicillin, ampicillin and streptomycin whereas less resistant to Ceftriaxone and Cefotaxime. The resistance patterns against azithromycin, ciprofloxacin, chloramphenicol, gentamycin, nalidixic acid and kanamycin were fluctuated from 25% to 71.43% among the isolates. This increasing development of multidrug resistance is alarming for the poultry industry and an increasing food safety concern for human. Asian Australas. J. Biosci. Biotechnol. 2018, 3 (1), 17-27
{"title":"Prevalence of multidrug resistant (MDR) food-borne pathogens in raw chicken meat in Dhaka city, Bangladesh: an increasing food safety concern","authors":"M. Islam, M. Sabrin, Md Kabir, S. Karim, Tahmina Sikder","doi":"10.3329/aajbb.v3i1.64747","DOIUrl":"https://doi.org/10.3329/aajbb.v3i1.64747","url":null,"abstract":"The present study was performed to investigate the prevalence of multidrug resistant (MDR) food-borne pathogens in raw chicken meat in Dhaka city, Bangladesh: an increasing food safety concern. A total of 100 meat samples (05 from each market) were collected from randomly selected 20 retail markets of Dhaka city using sterile polythene bags in a view to prevent extraneous contamination. The collected meat samples were then processed and inoculated onto nutrient broth and nutrient agar plates for isolation. The isolated organisms were identified based on staining, motility, cultural and biochemical properties according to standard laboratory methods. The isolated bacteria were also subjected to characterize their antibiotic sensitivity. In the present study, it was revealed that 100% of samples were contaminated by at least one species of bacteria belonging to 5 genera such as Staphylococcus, Escherichia coli, Salmonella, Enterobacter and Bacillus. Out of total 100 meat samples, 56% were contaminated with Escherichia coli whereas 42% were contaminated with Coagulase positive Staphylococcus and 36% were Salmonella species followed by 20%, 14% and 10% were contaminated with Enterobacter species, Coagulase negative Staphylococcus and Bacillus species respectively. A total of 174 bacteria were isolated and identified from raw chicken meat samples inspected of which 29.89% were Escherichia coli, 24.14% were Coagulase positive Staphylococcus, 20.69% were Salmonella species, 11.49% were Enterobacter species, 8.05% were Coagulase negative Staphylococcus and 5.75% were Bacillus species. The antimicrobial sensitivity tests showed that 96.15% of Escherichia coli (50 out of 52), 95.24% of Coagulase positive Staphylococcus (40 out of 42) and 86.11% of Salmonella (31 out of 36) isolates displayed multidrug resistance phenotypes (resistant to more than two antimicrobial agents). All most all the isolates of E. coli, coagulase positive Staphylococcus and Salmonella were more resistant to tetracycline, amoxicillin, ampicillin and streptomycin whereas less resistant to Ceftriaxone and Cefotaxime. The resistance patterns against azithromycin, ciprofloxacin, chloramphenicol, gentamycin, nalidixic acid and kanamycin were fluctuated from 25% to 71.43% among the isolates. This increasing development of multidrug resistance is alarming for the poultry industry and an increasing food safety concern for human. \u0000Asian Australas. J. Biosci. Biotechnol. 2018, 3 (1), 17-27","PeriodicalId":184754,"journal":{"name":"Asian-Australasian Journal of Bioscience and Biotechnology","volume":"43 4 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126756874","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-04-28DOI: 10.3329/aajbb.v3i1.64744
S. Akter, Hossain Kabir, Sreekanta Biswas, P. Paul, T. Hasan
The skin, sometimes known as the integumentary System is, in fact, the largest organ of the body. It performs many functions that are important in maintaining homeostasis in the body. Probably the most important of these functions is the control of body temperature. The skin also protects the body from physical damage and bacterial invasion. The skin has an array of sense organs which sense the external environment and also cells which can make vitamin D in sunlight. A study was undertaken of the prevalence, diagnosis and treatment of dermatological disorders in hospitalized dogs at Madras Veterinary College (MVC), Chennai, India from 1st May to 30th May, 2017. Total 220 cases were observed and recorded that had a dermatological problem. Among the dog pruritus (86%), alopecia (63%), scaling (77%), maculo-papular-pustular lesion (91%) were the most common presenting sign. A diagnosis or recommendation for treatment was done on the basis of the presenting clinical signs, physical examination and various diagnostic tests. In this study most of the cases were diagnosed by multiple and deep skin scrapings (100 cases) and by dermato-histopathology (70 cases). The most frequently diagnosed cases were Malassezia dermatitis, Demodicosis, Scabies and Tick infestation. Tick infestation accounted for the majority of the diagnoses. Systemic antibiotics were prescribed in 9% cases, systemic antifungal drugs were prescribed in 7% cases, systemic glucocorticoids were prescribed in 10% cases, antihistaminic in 6% cases and treatment with an ectoparasiticide was prescribed in 73% cases. Asian Australas. J. Biosci. Biotechnol. 2018, 3 (1), 1-6
{"title":"Study on prevalence, diagnosis and treatment of dermatological disorders in hospitalized dogs at Madras Veterinary College (MVC), Chennai, India","authors":"S. Akter, Hossain Kabir, Sreekanta Biswas, P. Paul, T. Hasan","doi":"10.3329/aajbb.v3i1.64744","DOIUrl":"https://doi.org/10.3329/aajbb.v3i1.64744","url":null,"abstract":"The skin, sometimes known as the integumentary System is, in fact, the largest organ of the body. It performs many functions that are important in maintaining homeostasis in the body. Probably the most important of these functions is the control of body temperature. The skin also protects the body from physical damage and bacterial invasion. The skin has an array of sense organs which sense the external environment and also cells which can make vitamin D in sunlight. A study was undertaken of the prevalence, diagnosis and treatment of dermatological disorders in hospitalized dogs at Madras Veterinary College (MVC), Chennai, India from 1st May to 30th May, 2017. Total 220 cases were observed and recorded that had a dermatological problem. Among the dog pruritus (86%), alopecia (63%), scaling (77%), maculo-papular-pustular lesion (91%) were the most common presenting sign. A diagnosis or recommendation for treatment was done on the basis of the presenting clinical signs, physical examination and various diagnostic tests. In this study most of the cases were diagnosed by multiple and deep skin scrapings (100 cases) and by dermato-histopathology (70 cases). The most frequently diagnosed cases were Malassezia dermatitis, Demodicosis, Scabies and Tick infestation. Tick infestation accounted for the majority of the diagnoses. Systemic antibiotics were prescribed in 9% cases, systemic antifungal drugs were prescribed in 7% cases, systemic glucocorticoids were prescribed in 10% cases, antihistaminic in 6% cases and treatment with an ectoparasiticide was prescribed in 73% cases. \u0000Asian Australas. J. Biosci. Biotechnol. 2018, 3 (1), 1-6","PeriodicalId":184754,"journal":{"name":"Asian-Australasian Journal of Bioscience and Biotechnology","volume":"54 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121577093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-04-28DOI: 10.3329/aajbb.v3i1.64749
N. Das, Md. Touhidul Islam, Md. Jamiul Islam, A. Adham
The study was conducted at the experimental field of the Bangladesh Agricultural University (BAU) to demonstrate the evidence of the suitability of dairy farm’s wastewater on soil properties in a maize field under three fertilizer doses and three irrigation treatments. Irrigation had three treatments - I1: Irrigation with fresh water, I2: Irrigation with mixed water (fresh water: dairy farm’s wastewater = 1:1) and I3: Irrigation with raw wastewater. There were three fertilizer treatments - F0: No fertilizer, F1: Half of recommended dose fertilizer and F2: Full dose fertilizer. Wastewater contained different nutrients and organic matter, which optimistically contributed to the soil in the maize field. Both irrigation and fertilizer treatments employed different degrees of influence on the soil health. For the effect of irrigation treatments, the highest values of EC (0.223 dS/m), pH (8.18), OC (0.3733 %), total N (0.046 %), P (7.65 ppm), K (33 .08 ppm), Ca (297.80 ppm) and Mg (109.9 ppm) were recorded under the treatment of I3, I1, I1, I1, I3, I1, I2 and I3, respectively, and the lowest values of the soil quality parameters were counted under the treatment of I1, I3, I3, I3, I2, I2, I1, and I2, respectively. In case of fertilizer treatments, the maximum values of the soil quality parameters were obtained under the treatment of F2, F2, F0, F1, F2, F1, F1, and F1, respectively, and the lowest values were obtained under the treatment of F0, F0, F1, F0, F0, F0, F0, F2, respectively. For the irrigation and fertilizer interactions, the maximum values of EC (0.250 dS/m), pH (8.20), OC (0.38 %), total N (0.059 %), P (8.37 ppm), K (48.0 ppm), Ca (374.9 ppm) and Mg (112.60 ppm) were recorded under the treatment combinations I3F0, I1F2, I3F2, I1F2, I3F2, I3F1, I2F0 and I3F2, respectively, and thye lowest values were recorded under the treatment combinations of I2F0, I1F0, I1F0, I1F0, I2F0, I2F0, I2F2 and I2F2, respectively. Both the irrigation and fertilizer treatments and their combinations did not cause any significant variation in the quality parameters of the soil, except soil pH and phosphorus (P) content of the soil in the maize field. Asian Australas. J. Biosci. Biotechnol. 2018, 3 (1), 33-39
{"title":"Response of dairy farm’s wastewater irrigation and fertilizer interactions to soil health for maize cultivation in Bangladesh","authors":"N. Das, Md. Touhidul Islam, Md. Jamiul Islam, A. Adham","doi":"10.3329/aajbb.v3i1.64749","DOIUrl":"https://doi.org/10.3329/aajbb.v3i1.64749","url":null,"abstract":"The study was conducted at the experimental field of the Bangladesh Agricultural University (BAU) to demonstrate the evidence of the suitability of dairy farm’s wastewater on soil properties in a maize field under three fertilizer doses and three irrigation treatments. Irrigation had three treatments - I1: Irrigation with fresh water, I2: Irrigation with mixed water (fresh water: dairy farm’s wastewater = 1:1) and I3: Irrigation with raw wastewater. There were three fertilizer treatments - F0: No fertilizer, F1: Half of recommended dose fertilizer and F2: Full dose fertilizer. Wastewater contained different nutrients and organic matter, which optimistically contributed to the soil in the maize field. Both irrigation and fertilizer treatments employed different degrees of influence on the soil health. For the effect of irrigation treatments, the highest values of EC (0.223 dS/m), pH (8.18), OC (0.3733 %), total N (0.046 %), P (7.65 ppm), K (33 .08 ppm), Ca (297.80 ppm) and Mg (109.9 ppm) were recorded under the treatment of I3, I1, I1, I1, I3, I1, I2 and I3, respectively, and the lowest values of the soil quality parameters were counted under the treatment of I1, I3, I3, I3, I2, I2, I1, and I2, respectively. In case of fertilizer treatments, the maximum values of the soil quality parameters were obtained under the treatment of F2, F2, F0, F1, F2, F1, F1, and F1, respectively, and the lowest values were obtained under the treatment of F0, F0, F1, F0, F0, F0, F0, F2, respectively. For the irrigation and fertilizer interactions, the maximum values of EC (0.250 dS/m), pH (8.20), OC (0.38 %), total N (0.059 %), P (8.37 ppm), K (48.0 ppm), Ca (374.9 ppm) and Mg (112.60 ppm) were recorded under the treatment combinations I3F0, I1F2, I3F2, I1F2, I3F2, I3F1, I2F0 and I3F2, respectively, and thye lowest values were recorded under the treatment combinations of I2F0, I1F0, I1F0, I1F0, I2F0, I2F0, I2F2 and I2F2, respectively. Both the irrigation and fertilizer treatments and their combinations did not cause any significant variation in the quality parameters of the soil, except soil pH and phosphorus (P) content of the soil in the maize field. \u0000Asian Australas. J. Biosci. Biotechnol. 2018, 3 (1), 33-39","PeriodicalId":184754,"journal":{"name":"Asian-Australasian Journal of Bioscience and Biotechnology","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133436759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-04-28DOI: 10.3329/aajbb.v3i1.64750
M. Islam, Md Ali Akbar, Md. Jasimuddin Khan, Md. Rezwanul Habib, Md Younus Ali, M. A. Islam
The objective of this study was to determine the effect of urea-molasses and di-calcium phosphate additives on silage quality and milk yield of cows. Hence, three types of silages were prepared from Napier fodder namely-Napier silage, urea and molasses added Napier silage (UMN); and di-calcium phosphate added Napier (DCPN) silage. These types of silage fed to the selected nine lactating Holstein-Friesian crossbred cows and divided into three groups considering three of each. Along with 5 kg of respective silages, ad-libitum rice straw and 3 kg concentrate mixture supplied to each group of cows. Physical attributes of all prepared silages were possessing characteristics of good quality silage. Dry matter content in UMN and DCPN silages were 1.67 and 1.01% higher than Napier silage. UMN silage had 1.59 and 2.33% lower ADF from Napier and DCPN silage, respectively. Again, 1.29% higher NH3-N presents in UMN silage than Napier silage. From 1st to 4th fortnight milk yield data it was found that 23.70, 23.19 and 27.06% milk production increased those cows received Napier, UMN and DCPN silage, respectively. Considering above these, it might be concluded that addition of di-calcium phosphate as additive during silage making would be beneficial for the dairy farmers. Asian Australas. J. Biosci. Biotechnol. 2018, 3 (1), 40-44
{"title":"Effect of adding different additives on silage quality and milk production of lactating crossbred cows","authors":"M. Islam, Md Ali Akbar, Md. Jasimuddin Khan, Md. Rezwanul Habib, Md Younus Ali, M. A. Islam","doi":"10.3329/aajbb.v3i1.64750","DOIUrl":"https://doi.org/10.3329/aajbb.v3i1.64750","url":null,"abstract":"The objective of this study was to determine the effect of urea-molasses and di-calcium phosphate additives on silage quality and milk yield of cows. Hence, three types of silages were prepared from Napier fodder namely-Napier silage, urea and molasses added Napier silage (UMN); and di-calcium phosphate added Napier (DCPN) silage. These types of silage fed to the selected nine lactating Holstein-Friesian crossbred cows and divided into three groups considering three of each. Along with 5 kg of respective silages, ad-libitum rice straw and 3 kg concentrate mixture supplied to each group of cows. Physical attributes of all prepared silages were possessing characteristics of good quality silage. Dry matter content in UMN and DCPN silages were 1.67 and 1.01% higher than Napier silage. UMN silage had 1.59 and 2.33% lower ADF from Napier and DCPN silage, respectively. Again, 1.29% higher NH3-N presents in UMN silage than Napier silage. From 1st to 4th fortnight milk yield data it was found that 23.70, 23.19 and 27.06% milk production increased those cows received Napier, UMN and DCPN silage, respectively. Considering above these, it might be concluded that addition of di-calcium phosphate as additive during silage making would be beneficial for the dairy farmers. \u0000Asian Australas. J. Biosci. Biotechnol. 2018, 3 (1), 40-44","PeriodicalId":184754,"journal":{"name":"Asian-Australasian Journal of Bioscience and Biotechnology","volume":"17 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"117059103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-04-28DOI: 10.3329/aajbb.v3i1.64754
Md. Nur Islam, Md. Shajedur Rahman, M. Islam, Md Fazlul Hoque, N. Rumi
The experiment was conducted to evaluate the effects of growth promoter (Aviator™) and enzymes (Acinor™) with basal feed. A total of 40 Cobb-500 Broiler day old chicks were reared in an open sided gable type house for a period of 5 weeks from 24th september to 28th october, 2017. Body weight and feed intake were recorded on day 0, 7, 14, 21, 28 and 35. The experiment was conducted in a Completely Randomized Design (CRD). Birds were randomly distributed into four dietary groups i.e control (T0) with basal diet, T1 with basal diet and growth promoter (GP) @ 2 gm/kg of feed; T2 with basal diet and enzymes (EZ) @ 0.4 gm/kg of feed and T3 with basal diet and GP plus EZ (2 gm/kg + 0.4 gm/kg). The records were kept on body weight, feed intake and mortality while weight gain, feed conversion ratio (FCR) and survivability were calculated. Broiler chicks that received GP and a combination of GP+EZ treatments showed significant improvement in performance (p<0.05) over control with respect to body weight gain, feed conversion ratio, carcass yield. Cost-effectiveness of GP+EZ treatment higher than all other treatments but GP and EZ had almost similar cost-effectiveness. Feeding EZ alone had comparatively less weight gain and almost similar feed conversion ratio compared with GP groups but it’s performance was significantly better than that of control group. This study indicated that the diet containing GP+EZ offered slightly increased benefits to the growth performance of broilers and these benefits were almost equal to the GP. It is revealed that growth promoter (Aviator™) supplementation with enzymes (Acinor™) is beneficial for broiler production. Asian Australas. J. Biosci. Biotechnol. 2018, 3 (1), 59-66
本试验旨在评价基础饲料中添加生长促进剂(Aviator™)和酶(Acinor™)的效果。试验于2017年9月24日至10月28日在开放式山墙式鸡舍中饲养40只Cobb-500日龄肉鸡,为期5周。分别于第0、7、14、21、28和35天记录体重和采食量。试验采用完全随机设计(CRD)。将试验禽随机分为4个饲粮组:对照组(T0)饲喂基础饲粮,T1组饲喂基础饲粮和生长促进剂(GP) @ 2 gm/kg饲料;T2组饲喂基础饲粮+酶(EZ) @ 0.4 gm/kg饲料,T3组饲喂基础饲粮+ GP + EZ (2 gm/kg + 0.4 gm/kg)。记录体重、采食量和死亡率,计算增重、饲料系数和存活率。GP和GP+EZ组合处理的肉鸡在增重、饲料系数、胴体产量方面均显著高于对照组(p<0.05)。GP+EZ治疗的成本-效果高于所有其他治疗,但GP和EZ的成本-效果几乎相似。单独饲喂EZ的增重相对较低,饲料系数与GP组相近,但生产性能显著优于对照组。本研究表明,饲粮中添加GP+EZ对肉鸡生长性能的效益略有提高,且与GP的效益基本相当。结果表明,在生长促进剂(Aviator™)中添加酶(Acinor™)有利于肉鸡生产。亚洲Australas。j . Biosci。生物技术,2018,3 (1),59-66
{"title":"Comparative study on feeding of growth promoter (Aviator™) and enzymes (Acinor™) on growth performance of broiler","authors":"Md. Nur Islam, Md. Shajedur Rahman, M. Islam, Md Fazlul Hoque, N. Rumi","doi":"10.3329/aajbb.v3i1.64754","DOIUrl":"https://doi.org/10.3329/aajbb.v3i1.64754","url":null,"abstract":"The experiment was conducted to evaluate the effects of growth promoter (Aviator™) and enzymes (Acinor™) with basal feed. A total of 40 Cobb-500 Broiler day old chicks were reared in an open sided gable type house for a period of 5 weeks from 24th september to 28th october, 2017. Body weight and feed intake were recorded on day 0, 7, 14, 21, 28 and 35. The experiment was conducted in a Completely Randomized Design (CRD). Birds were randomly distributed into four dietary groups i.e control (T0) with basal diet, T1 with basal diet and growth promoter (GP) @ 2 gm/kg of feed; T2 with basal diet and enzymes (EZ) @ 0.4 gm/kg of feed and T3 with basal diet and GP plus EZ (2 gm/kg + 0.4 gm/kg). The records were kept on body weight, feed intake and mortality while weight gain, feed conversion ratio (FCR) and survivability were calculated. Broiler chicks that received GP and a combination of GP+EZ treatments showed significant improvement in performance (p<0.05) over control with respect to body weight gain, feed conversion ratio, carcass yield. Cost-effectiveness of GP+EZ treatment higher than all other treatments but GP and EZ had almost similar cost-effectiveness. Feeding EZ alone had comparatively less weight gain and almost similar feed conversion ratio compared with GP groups but it’s performance was significantly better than that of control group. This study indicated that the diet containing GP+EZ offered slightly increased benefits to the growth performance of broilers and these benefits were almost equal to the GP. It is revealed that growth promoter (Aviator™) supplementation with enzymes (Acinor™) is beneficial for broiler production. \u0000Asian Australas. J. Biosci. Biotechnol. 2018, 3 (1), 59-66","PeriodicalId":184754,"journal":{"name":"Asian-Australasian Journal of Bioscience and Biotechnology","volume":"9 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128350084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}