Pub Date : 2024-09-10DOI: 10.1186/s43043-024-00204-7
Thuy Thi-Thanh Tran, Huy Phuong Tran, Loc Thai Ly, Tuyet Thi-Diem Hoang, Trang Nguyen-Khanh Huynh, Son Truong Dang
Endometrial preparation significantly influences the success of embryo transfer procedures. Although both oral and transdermal estradiol are common methods for endometrial priming, their efficacy and potential side effects remain uncertain. This randomized controlled trial aims to compare the effectiveness of oral and transdermal estradiol in endometrial preparation, while also evaluating their respective side effects in patients undergoing assisted reproduction treatments. This randomized clinical trial (ISRCTN15301227) was conducted at Hung Vuong Hospital between July 2020 and March 2022. Among 550 eligible patients undergoing frozen embryo transfer cycles, we included 380 patients for the study. The study protocol and all materials received approval from the Ethics Committee of Hung Vuong Hospital (1315/CN-HĐĐĐ). Participants were randomly assigned to one of two groups: Group A (n =190) received oral estradiol at an initial dose of 4 mg per day for 7 days, with the dose increased according to clinical response. Group B (n =190) received transdermal estradiol at an initial dose of 2 measures of 2.5 g estradiol gel per day for 7 days, with the dose similarly increased according to clinical response. Treatment in both groups began on days 2–3 of the menstrual cycle, with the maximum duration of estradiol administration being 27 days. We compared estradiol levels on the day of progesterone administration, duration of treatment, total estradiol dose, endometrial thickness, pregnancy outcomes, and any observed side effects between the two groups. Group A exhibited significantly higher estradiol levels on the day of progesterone administration compared to Group B (270.5 pg/ml versus 186.5 pg/ml, p < 0.001). However, the comparison revealed no significant difference in endometrial thickness between the two groups (10.5 mm versus 10.6 mm, p = 0.85). Furthermore, pregnancy rates including positive human chorionic gonadotropin, clinical pregnancy, ongoing pregnancy, live birth, and pregnancy failure were also found to be similar between the two groups. Notably, a greater proportion of patients in Group A experienced mild side effects compared to those in Group B (20.3% versus 10.1%, respectively; n =37 versus n =18), and this discrepancy was found to be statistically significant (p = 0.007). Transdermal estradiol offers comparable endometrial thickness and pregnancy outcomes, along with improved patient compliance and fewer side effects compared to oral estradiol.
{"title":"Evaluating the effectiveness and adverse effects of oral versus transdermal estradiol for endometrial preparation in frozen-thawed embryo transfer: a randomized controlled trial","authors":"Thuy Thi-Thanh Tran, Huy Phuong Tran, Loc Thai Ly, Tuyet Thi-Diem Hoang, Trang Nguyen-Khanh Huynh, Son Truong Dang","doi":"10.1186/s43043-024-00204-7","DOIUrl":"https://doi.org/10.1186/s43043-024-00204-7","url":null,"abstract":"Endometrial preparation significantly influences the success of embryo transfer procedures. Although both oral and transdermal estradiol are common methods for endometrial priming, their efficacy and potential side effects remain uncertain. This randomized controlled trial aims to compare the effectiveness of oral and transdermal estradiol in endometrial preparation, while also evaluating their respective side effects in patients undergoing assisted reproduction treatments. This randomized clinical trial (ISRCTN15301227) was conducted at Hung Vuong Hospital between July 2020 and March 2022. Among 550 eligible patients undergoing frozen embryo transfer cycles, we included 380 patients for the study. The study protocol and all materials received approval from the Ethics Committee of Hung Vuong Hospital (1315/CN-HĐĐĐ). Participants were randomly assigned to one of two groups: Group A (n =190) received oral estradiol at an initial dose of 4 mg per day for 7 days, with the dose increased according to clinical response. Group B (n =190) received transdermal estradiol at an initial dose of 2 measures of 2.5 g estradiol gel per day for 7 days, with the dose similarly increased according to clinical response. Treatment in both groups began on days 2–3 of the menstrual cycle, with the maximum duration of estradiol administration being 27 days. We compared estradiol levels on the day of progesterone administration, duration of treatment, total estradiol dose, endometrial thickness, pregnancy outcomes, and any observed side effects between the two groups. Group A exhibited significantly higher estradiol levels on the day of progesterone administration compared to Group B (270.5 pg/ml versus 186.5 pg/ml, p < 0.001). However, the comparison revealed no significant difference in endometrial thickness between the two groups (10.5 mm versus 10.6 mm, p = 0.85). Furthermore, pregnancy rates including positive human chorionic gonadotropin, clinical pregnancy, ongoing pregnancy, live birth, and pregnancy failure were also found to be similar between the two groups. Notably, a greater proportion of patients in Group A experienced mild side effects compared to those in Group B (20.3% versus 10.1%, respectively; n =37 versus n =18), and this discrepancy was found to be statistically significant (p = 0.007). Transdermal estradiol offers comparable endometrial thickness and pregnancy outcomes, along with improved patient compliance and fewer side effects compared to oral estradiol.","PeriodicalId":18532,"journal":{"name":"Middle East Fertility Society Journal","volume":"83 1","pages":""},"PeriodicalIF":1.3,"publicationDate":"2024-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142179940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-08-24DOI: 10.1186/s43043-024-00201-w
Mohsen Kazeminia, Fatemeh Rajati, Roumina Rasulehvandi, Mojgan Rajati
Polycystic ovarian syndrome (PCOS) is recognized as the most common endocrine disorder among women of reproductive age and the most common cause of infertility. Given the importance of the subject and the inconsistency of the results of the primary studies, the present study aimed at estimating the pooled effect of vitamin D on the hormonal profile of women with PCOS using systematic review and meta-analysis. A systematic literature review was performed in PubMed, Scopus, Embase, Web of Science (WoS), Cochrane, ClinicalTrials.gov databases, and Google Scholar motor engine using related Medical Subject Headings (MeSH) and Free Text words with no time limit to April 2022. Heterogeneity among studies was quantified using I2 index. After eliminating duplicates and irrelevant studies, ultimately, 19 articles with a sample size of 450 in the intervention group and 450 in the control group were included in the meta-analysis. As a result of the combination of studies, mean the standardized difference (SMD) before and after the intervention was obtained 0.241 ± 0.098 for dehydroepiandrosterone sulfate (DHEAS), 0.330 ± 0.092 for sex hormone-binding globulin (SHBG), 0.707 ± 0.171 for testosterone, 0.614 ± 0.199 for luteinizing hormone (LH), 0.220 ± 0.119 for follicle-stimulating hormone (FSH), 0.655 ± 0.505 for anti-Müllerian hormone (AMH), and 0.369 ± 0.109 for Free Androgen Index (FAI) in the intervention group compared to the control group. The results indicated that 8-week interventions had a greater positive effect than 12-week interventions. The results of the current meta-analysis revealed a significant positive effect of vitamin D on the hormonal profile of women with PCOS, which should be considered by obstetricians and midwives.
{"title":"The effect of vitamin D on the hormonal profile of women with polycystic ovarian syndrome: a systematic review and meta-analysis","authors":"Mohsen Kazeminia, Fatemeh Rajati, Roumina Rasulehvandi, Mojgan Rajati","doi":"10.1186/s43043-024-00201-w","DOIUrl":"https://doi.org/10.1186/s43043-024-00201-w","url":null,"abstract":"Polycystic ovarian syndrome (PCOS) is recognized as the most common endocrine disorder among women of reproductive age and the most common cause of infertility. Given the importance of the subject and the inconsistency of the results of the primary studies, the present study aimed at estimating the pooled effect of vitamin D on the hormonal profile of women with PCOS using systematic review and meta-analysis. A systematic literature review was performed in PubMed, Scopus, Embase, Web of Science (WoS), Cochrane, ClinicalTrials.gov databases, and Google Scholar motor engine using related Medical Subject Headings (MeSH) and Free Text words with no time limit to April 2022. Heterogeneity among studies was quantified using I2 index. After eliminating duplicates and irrelevant studies, ultimately, 19 articles with a sample size of 450 in the intervention group and 450 in the control group were included in the meta-analysis. As a result of the combination of studies, mean the standardized difference (SMD) before and after the intervention was obtained 0.241 ± 0.098 for dehydroepiandrosterone sulfate (DHEAS), 0.330 ± 0.092 for sex hormone-binding globulin (SHBG), 0.707 ± 0.171 for testosterone, 0.614 ± 0.199 for luteinizing hormone (LH), 0.220 ± 0.119 for follicle-stimulating hormone (FSH), 0.655 ± 0.505 for anti-Müllerian hormone (AMH), and 0.369 ± 0.109 for Free Androgen Index (FAI) in the intervention group compared to the control group. The results indicated that 8-week interventions had a greater positive effect than 12-week interventions. The results of the current meta-analysis revealed a significant positive effect of vitamin D on the hormonal profile of women with PCOS, which should be considered by obstetricians and midwives.","PeriodicalId":18532,"journal":{"name":"Middle East Fertility Society Journal","volume":"5 1","pages":""},"PeriodicalIF":1.3,"publicationDate":"2024-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142179941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-08-20DOI: 10.1186/s43043-024-00203-8
Bonaventure Michael Ukoaka, Olalekan John Okesanya, Monica Anurika Gbuchie, Faithful Miebaka Daniel, Tajuddeen Adam Wali, Winner Chimdindu Ugorji, Ntishor Gabriel Udam, Na’ima Tanimu Abubakar, Don Lucero-Prisno Eliseo
Historically, viruses have impaired the functionality of human systems. The discovery of novel viruses and the resurgence of established strains heighten concerns about their implications for reproduction. In the aftermath of COVID-19, research efforts have intensified to broaden the understanding of the severe acute respiratory syndrome coronavirus-2’s (SARS-CoV-2) effect on male reproduction across diverse populations. Various findings have been reported, with more studies highlighting the adverse impact of SARS-CoV-2 on semen parameters and, consequently, male fertility. This review aims to comprehensively assess and consolidate existing evidence on the impact of SARS-CoV-2 on semen quality and male fertility. It highlights the potential mechanism of action and further underscores the implications for assisted reproductive technology. A thorough literature search was conducted across various electronic databases, including PubMed, Scopus, Google Scholar, Embase, and Web of Science. Studies published between January 2020 and May 2024 were included if they explored the impact of SARS-CoV-2 on semen quality. Twenty-nine (29) studies were included in the review. These studies varied in findings but delineated a pattern and trend. While most studies noted a decline in sperm parameters—motility, count, concentration—altered morphology, hormonal imbalances, and increased DNA fragmentation in COVID-19 patients, others reported normal semen parameters 3 to 6 months post-recovery. Few studies reported no change in semen parameters, especially with mild disease. Potential mechanisms underscoring these effects include the presence of fever and consequent release of pro-inflammatory cytokines—interleukin 1β, tumor necrosis factor (TNF), and interferon-gamma (IFNγ). In addition, the activities of the angiotensin-converting enzyme 2 (ACE2) and the transmembrane serine protease 2 (TMPRSS2) receptors have been implicated as gateway mechanisms for viral entry. The long-term consequences and comparisons with other viral infections highlight the complexity of drawing definite conclusions. The different findings on semen changes have implications for assisted reproduction and family planning. Research suggests potential negative effects on gonadal function, emphasizing the need for long-term follow-up studies to understand the persistent effects on male fertility biomarkers. A multidisciplinary approach is essential to optimizing male reproductive health during and after SARS-CoV-2 infection. This includes incorporating assessments into vaccine safety studies to address fertility concerns.
{"title":"Semen in the time of COVID-19: a narrative review of current evidence and implications for fertility and reproductive health","authors":"Bonaventure Michael Ukoaka, Olalekan John Okesanya, Monica Anurika Gbuchie, Faithful Miebaka Daniel, Tajuddeen Adam Wali, Winner Chimdindu Ugorji, Ntishor Gabriel Udam, Na’ima Tanimu Abubakar, Don Lucero-Prisno Eliseo","doi":"10.1186/s43043-024-00203-8","DOIUrl":"https://doi.org/10.1186/s43043-024-00203-8","url":null,"abstract":"Historically, viruses have impaired the functionality of human systems. The discovery of novel viruses and the resurgence of established strains heighten concerns about their implications for reproduction. In the aftermath of COVID-19, research efforts have intensified to broaden the understanding of the severe acute respiratory syndrome coronavirus-2’s (SARS-CoV-2) effect on male reproduction across diverse populations. Various findings have been reported, with more studies highlighting the adverse impact of SARS-CoV-2 on semen parameters and, consequently, male fertility. This review aims to comprehensively assess and consolidate existing evidence on the impact of SARS-CoV-2 on semen quality and male fertility. It highlights the potential mechanism of action and further underscores the implications for assisted reproductive technology. A thorough literature search was conducted across various electronic databases, including PubMed, Scopus, Google Scholar, Embase, and Web of Science. Studies published between January 2020 and May 2024 were included if they explored the impact of SARS-CoV-2 on semen quality. Twenty-nine (29) studies were included in the review. These studies varied in findings but delineated a pattern and trend. While most studies noted a decline in sperm parameters—motility, count, concentration—altered morphology, hormonal imbalances, and increased DNA fragmentation in COVID-19 patients, others reported normal semen parameters 3 to 6 months post-recovery. Few studies reported no change in semen parameters, especially with mild disease. Potential mechanisms underscoring these effects include the presence of fever and consequent release of pro-inflammatory cytokines—interleukin 1β, tumor necrosis factor (TNF), and interferon-gamma (IFNγ). In addition, the activities of the angiotensin-converting enzyme 2 (ACE2) and the transmembrane serine protease 2 (TMPRSS2) receptors have been implicated as gateway mechanisms for viral entry. The long-term consequences and comparisons with other viral infections highlight the complexity of drawing definite conclusions. The different findings on semen changes have implications for assisted reproduction and family planning. Research suggests potential negative effects on gonadal function, emphasizing the need for long-term follow-up studies to understand the persistent effects on male fertility biomarkers. A multidisciplinary approach is essential to optimizing male reproductive health during and after SARS-CoV-2 infection. This includes incorporating assessments into vaccine safety studies to address fertility concerns.","PeriodicalId":18532,"journal":{"name":"Middle East Fertility Society Journal","volume":"12 1","pages":""},"PeriodicalIF":1.3,"publicationDate":"2024-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142179942","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
With the increased next generation sequencing (NGS) based genetic diagnosis due to technological boon, the biomedical world is getting a substantial number of single nucleotide variations (SNVs) every day along with other genetic variations. The detected SNVs may or may not have clinical significance. Based on different levels of study, these SNVs are categorized either as disease associated or not disease associated. However, there exists another category called as “uncertain” where the scientific literature has scanty of data. These “uncertain” or “variants of uncertain significance (VUS)” has become the greatest challenge for the diagnostic fraternity since no specific decision can be taken by them for the persons carrying the VUS. Therefore, there exists a huge knowledge gap that needs to be addressed for better patient care. The present study aims to find out the possible ways of investigation that may help in reducing this knowledge gap so that decisive approaches can be made against VUS for better and accurate patient care.
{"title":"From uncertain to certain—how to proceed with variants of uncertain significance","authors":"Emili Banerjee, Suman Pal, Abhijit Biswas, Koutilya Bhattacharjee","doi":"10.1186/s43043-024-00202-9","DOIUrl":"https://doi.org/10.1186/s43043-024-00202-9","url":null,"abstract":"With the increased next generation sequencing (NGS) based genetic diagnosis due to technological boon, the biomedical world is getting a substantial number of single nucleotide variations (SNVs) every day along with other genetic variations. The detected SNVs may or may not have clinical significance. Based on different levels of study, these SNVs are categorized either as disease associated or not disease associated. However, there exists another category called as “uncertain” where the scientific literature has scanty of data. These “uncertain” or “variants of uncertain significance (VUS)” has become the greatest challenge for the diagnostic fraternity since no specific decision can be taken by them for the persons carrying the VUS. Therefore, there exists a huge knowledge gap that needs to be addressed for better patient care. The present study aims to find out the possible ways of investigation that may help in reducing this knowledge gap so that decisive approaches can be made against VUS for better and accurate patient care.","PeriodicalId":18532,"journal":{"name":"Middle East Fertility Society Journal","volume":"7 1","pages":""},"PeriodicalIF":1.3,"publicationDate":"2024-08-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142179943","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-24DOI: 10.1186/s43043-024-00187-5
Achmad Kemal Harzif, Sarah Miriam Ratna Pratamasari, Costan Tryono Parulian Rumapea, Nafi’atul Ummah, Heidi Dewi Mutia, Putri Nurbaeti, Aisyah Retno Puspawardani, Budi Wiweko
Polycystic ovary syndrome (PCOS) is a complex endocrine condition prevalent among a significant number of women during their reproductive years. Remarkably, 90–95% of women seeking infertility solutions due to anovulation are diagnosed with PCOS. Luteal phase support (LPS) is a crucial aspect of assisted reproductive technologies (ART). This systematic review aimed to evaluate the effectiveness of LPS in women with PCOS undergoing ART, with a focus on pregnancy rates as the primary endpoint. A systematic search was conducted on EMBASE, PubMed, and Scopus databases without language restrictions. We searched for studies up to August 1, 2023. The search strategy used terms related to PCOS and LPS. Clinical trials and cohort studies involving infertile women with PCOS undergoing ART were included. The Risk of Bias 2 (ROB2) and the Newcastle-Ottawa Scale (NOS) tool were used to assess the risk of bias. The review included five studies comprising a total of 818 patients. The studies used various ovulation induction medications, such as letrozole, clomiphene citrate, and human menopausal gonadotropin, in combination with different forms of progesterone for LPS (oral, intramuscular, and intravaginal). The overall results demonstrated inconsistent efficacy of LPS, with some studies showing significant improvements in pregnancy rates with LPS, while others showed no statistically significant difference. The systematic review suggests that LPS may improve pregnancy rates in women with PCOS undergoing ART. However, the effectiveness appears to be influenced by the choice of ovulation induction agent and the route of progesterone administration. Personalized treatment approaches considering patient response and emerging evidence are essential.
{"title":"The efficacy of luteal phase support in women with polycystic ovary syndrome following assisted reproductive technology: a systematic review","authors":"Achmad Kemal Harzif, Sarah Miriam Ratna Pratamasari, Costan Tryono Parulian Rumapea, Nafi’atul Ummah, Heidi Dewi Mutia, Putri Nurbaeti, Aisyah Retno Puspawardani, Budi Wiweko","doi":"10.1186/s43043-024-00187-5","DOIUrl":"https://doi.org/10.1186/s43043-024-00187-5","url":null,"abstract":"Polycystic ovary syndrome (PCOS) is a complex endocrine condition prevalent among a significant number of women during their reproductive years. Remarkably, 90–95% of women seeking infertility solutions due to anovulation are diagnosed with PCOS. Luteal phase support (LPS) is a crucial aspect of assisted reproductive technologies (ART). This systematic review aimed to evaluate the effectiveness of LPS in women with PCOS undergoing ART, with a focus on pregnancy rates as the primary endpoint. A systematic search was conducted on EMBASE, PubMed, and Scopus databases without language restrictions. We searched for studies up to August 1, 2023. The search strategy used terms related to PCOS and LPS. Clinical trials and cohort studies involving infertile women with PCOS undergoing ART were included. The Risk of Bias 2 (ROB2) and the Newcastle-Ottawa Scale (NOS) tool were used to assess the risk of bias. The review included five studies comprising a total of 818 patients. The studies used various ovulation induction medications, such as letrozole, clomiphene citrate, and human menopausal gonadotropin, in combination with different forms of progesterone for LPS (oral, intramuscular, and intravaginal). The overall results demonstrated inconsistent efficacy of LPS, with some studies showing significant improvements in pregnancy rates with LPS, while others showed no statistically significant difference. The systematic review suggests that LPS may improve pregnancy rates in women with PCOS undergoing ART. However, the effectiveness appears to be influenced by the choice of ovulation induction agent and the route of progesterone administration. Personalized treatment approaches considering patient response and emerging evidence are essential.","PeriodicalId":18532,"journal":{"name":"Middle East Fertility Society Journal","volume":"44 1","pages":""},"PeriodicalIF":1.3,"publicationDate":"2024-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141780071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-23DOI: 10.1186/s43043-024-00188-4
Mohammad Ali Khalili, Mahboubeh Vatanparast, Esmat Mangoli, Saeed Ghasmi-Esmaeilabad, Mojgan Moshrefi, Akram Hosseini
Classic vitrification methods are not appropriate when there are minimal numbers of viable sperm, and the new methods emphasize the low semen volumes in these cases. The aim was to assess the efficacy of the cryotech as a device for freezing low sperm volume, through the two methods of open (OVS) and closed (CVS) vitrification systems. Testicular biopsy samples from 30 men with obstructive azoospermia (OA) were assigned to three groups fresh control (FC), OVS, and CVS. Testicular sperms were selected using an ICSI injection pipette and vitrified on the cryotech straws, containing one droplet of freezing medium. After warming, sperm head morphometric characterizations were evaluated with the MSOME technique. Sperm motility, membrane integrity, chromatin quality assessment including DNA fragmentation, Chromomycine A3 staining (CMA3), and Aniline Blue (AB) were assessed. Fluorescein isothiocyanate-conjugated Pisum sativum agglutinin (FITC-PSA) was done to examine sperm acrosome integrity. The mean sperm motility, viability, and sperm with intact acrosome reduced after vitrification, in both methods of CVS, and OVS, but the results were more promising in the closed method (p < 0.05). However, the variations were not significant between the two methods of cryopreservation, the OVS undergoes significant head dimensions changes compared to the control group (p < 0.05). The results also showed higher membrane, and chromatin abnormality after OVS (p < 0.05). The overall post-thaw recovery of human testicular sperm proposes that CVS is more efficient for single sperm cryopreservation, while higher sperm viability, and lower alterations in chromatin, acrosome, and sperm head morphometry were seen compared to OVS.
{"title":"Comparison between open and closed systems for vitrification of individual sperm: assessing morphometric measurements and chromatin integrity","authors":"Mohammad Ali Khalili, Mahboubeh Vatanparast, Esmat Mangoli, Saeed Ghasmi-Esmaeilabad, Mojgan Moshrefi, Akram Hosseini","doi":"10.1186/s43043-024-00188-4","DOIUrl":"https://doi.org/10.1186/s43043-024-00188-4","url":null,"abstract":"Classic vitrification methods are not appropriate when there are minimal numbers of viable sperm, and the new methods emphasize the low semen volumes in these cases. The aim was to assess the efficacy of the cryotech as a device for freezing low sperm volume, through the two methods of open (OVS) and closed (CVS) vitrification systems. Testicular biopsy samples from 30 men with obstructive azoospermia (OA) were assigned to three groups fresh control (FC), OVS, and CVS. Testicular sperms were selected using an ICSI injection pipette and vitrified on the cryotech straws, containing one droplet of freezing medium. After warming, sperm head morphometric characterizations were evaluated with the MSOME technique. Sperm motility, membrane integrity, chromatin quality assessment including DNA fragmentation, Chromomycine A3 staining (CMA3), and Aniline Blue (AB) were assessed. Fluorescein isothiocyanate-conjugated Pisum sativum agglutinin (FITC-PSA) was done to examine sperm acrosome integrity. The mean sperm motility, viability, and sperm with intact acrosome reduced after vitrification, in both methods of CVS, and OVS, but the results were more promising in the closed method (p < 0.05). However, the variations were not significant between the two methods of cryopreservation, the OVS undergoes significant head dimensions changes compared to the control group (p < 0.05). The results also showed higher membrane, and chromatin abnormality after OVS (p < 0.05). The overall post-thaw recovery of human testicular sperm proposes that CVS is more efficient for single sperm cryopreservation, while higher sperm viability, and lower alterations in chromatin, acrosome, and sperm head morphometry were seen compared to OVS.","PeriodicalId":18532,"journal":{"name":"Middle East Fertility Society Journal","volume":"23 1","pages":""},"PeriodicalIF":1.3,"publicationDate":"2024-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141753904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In the current era of assisted reproductive technology, the strategy of single vitrified-warmed blastocyst transfer (SVBT) is acknowledged for positively impacting clinical outcomes and preventing the risk of multiple conceptions. Previous studies have indicated that blastocyst grade and the day of blastocyst positively correlate with outcomes. Moreover, storage duration has no significant effect on survival rates, clinical outcomes, and neonatal outcomes. However, some researchers express controversial opinions on cryostorage duration, and their findings suggest a negative impact on clinical outcomes. These results remain subject to controversy, and limited studies exist regarding the outcomes after SVBT. Therefore, our study aims to investigate the impact of the day of blastocyst, blastocyst grade, and blastocyst cryostorage duration on clinical and neonatal outcomes following SVBT in patients who underwent clomiphene-citrate-based minimal stimulation. In this study, retrospective cohort study data collected from June 2015 to April 2023 included 2107 patients with first SVBT cycles who underwent a clomiphene-based minimal ovarian stimulation protocol or a drug-free natural protocol at the Ojinmed IVF Center. Patients were categorized into four groups based on blastocyst cryostorage duration: group 1 (< 2 months, n = 882), group 2 (3–6 months, n = 794), group 3 (7–12 months, n = 187), group 4 (13–24 months, n = 126), and group 5 (25–81 months, n = 118). The patient’s clinical and neonatal outcomes were compared with cryostorage duration after the propensity score matched. Multivariable logistic regression analysis revealed that prolonged cryostorage duration insignificantly correlated with clinical outcomes. Additionally, neonatal outcomes are not correlated with cryostorage duration. The patient must consider several parameters when selecting embryos for transfer, including the duration of cryostorage. Our study results show that for the first single vitrified-warmed blastocyst transfer of patients who underwent clomiphene citrate-based minimal stimulation, cryostorage duration does not affect outcomes.
{"title":"Noneffectiveness of cryostorage duration on clinical and neonatal outcomes after single vitrified-warmed blastocyst transfers","authors":"Mungunshagai Baatarsuren, Jambaldorj Jamiyansuren, Chinzorig Ganbaatar, Davaakhuu Sengebaljir, Belguune Erdenekhuyag, Sandag Enkhbaatar, Namsrai Mungunsuvd, Lkhagvasuren Baljinnyam, Ganjiguur Tumur-Ochir, Ariunaa Amarsaikhan, Amarjargal Dorjpurev, Gereltsetseg Ganbat, Tsogzolmaa Boris, Azjargal Khangarid","doi":"10.1186/s43043-024-00196-4","DOIUrl":"https://doi.org/10.1186/s43043-024-00196-4","url":null,"abstract":"In the current era of assisted reproductive technology, the strategy of single vitrified-warmed blastocyst transfer (SVBT) is acknowledged for positively impacting clinical outcomes and preventing the risk of multiple conceptions. Previous studies have indicated that blastocyst grade and the day of blastocyst positively correlate with outcomes. Moreover, storage duration has no significant effect on survival rates, clinical outcomes, and neonatal outcomes. However, some researchers express controversial opinions on cryostorage duration, and their findings suggest a negative impact on clinical outcomes. These results remain subject to controversy, and limited studies exist regarding the outcomes after SVBT. Therefore, our study aims to investigate the impact of the day of blastocyst, blastocyst grade, and blastocyst cryostorage duration on clinical and neonatal outcomes following SVBT in patients who underwent clomiphene-citrate-based minimal stimulation. In this study, retrospective cohort study data collected from June 2015 to April 2023 included 2107 patients with first SVBT cycles who underwent a clomiphene-based minimal ovarian stimulation protocol or a drug-free natural protocol at the Ojinmed IVF Center. Patients were categorized into four groups based on blastocyst cryostorage duration: group 1 (< 2 months, n = 882), group 2 (3–6 months, n = 794), group 3 (7–12 months, n = 187), group 4 (13–24 months, n = 126), and group 5 (25–81 months, n = 118). The patient’s clinical and neonatal outcomes were compared with cryostorage duration after the propensity score matched. Multivariable logistic regression analysis revealed that prolonged cryostorage duration insignificantly correlated with clinical outcomes. Additionally, neonatal outcomes are not correlated with cryostorage duration. The patient must consider several parameters when selecting embryos for transfer, including the duration of cryostorage. Our study results show that for the first single vitrified-warmed blastocyst transfer of patients who underwent clomiphene citrate-based minimal stimulation, cryostorage duration does not affect outcomes.","PeriodicalId":18532,"journal":{"name":"Middle East Fertility Society Journal","volume":"54 1","pages":""},"PeriodicalIF":1.3,"publicationDate":"2024-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141587343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-10DOI: 10.1186/s43043-024-00198-2
Hakki Uzun, Görkem Akça, Berat Sönmez, Erdem Orman, Yakup Kaçan, Eyüp Dil
This bibliometric study aims to examine the associations of journals in the field of human reproduction with their access types and article processing charges to evaluate the evolving landscape of publishing in human reproduction. The primary databases, including Clarivate Analytics Master Journal List, Scopus®, PubMed, and Directory of Open Access Journals, were scrutinized to identify pertinent journals within the realm of human reproduction, utilizing keywords such as reproductive, reproduction, fertility, and infertility. Journals were excluded if they were not actively publishing in English or primarily focused on reproductive health, men’s health, sexual medicine, embryogenesis, developmental biology, or veterinary medicine concerning animal reproduction. A thorough characterization of the journals was conducted, followed by a comparative analysis of citation metrics and article processing charges across various access models. Forty-one journals were included into the study. A significant increase in the proportion of gold and diamond open-access journals was observed, rising from 42% (13 out of 31) to 53.6% (22 out of 41) by 2023. Hybrid journals demonstrated superior citation metrics compared to diamond open-access journals. For hybrid journals, a statistically significant, moderately positive correlation was found between article processing charges and CiteScore (rs (27) = 0.515, p < .024). Conversely, no correlation was observed between article processing charges and CiteScore for gold open-access journals (rs (27) = 0.445, p = 0.147). The mean article processing charges for all hybrid and gold open-access journals were calculated as US $3032.88 ± 1108.514 (312 to 4430). Specifically, the mean article processing charges for hybrid journals (US $3617.4 ± 610.19) were significantly higher than those for gold open-access journals (US $1916.82 ± 988.32), with a difference of 1700.658 (95% CI: 1124.861–2276.455), t (30) = 6.032, and p < .0005. Hybrid journals in the field of human reproduction carry fees nearly twice as high as those of gold open-access journals. The charging policies of gold open-access journals, which are not contigent upon citation metrics, emphasize the importance of caution for both authors and funders.
{"title":"The evolving landscape of publishing in human reproduction: an analysis of scientometric data, open-access publishing, and article processing charges","authors":"Hakki Uzun, Görkem Akça, Berat Sönmez, Erdem Orman, Yakup Kaçan, Eyüp Dil","doi":"10.1186/s43043-024-00198-2","DOIUrl":"https://doi.org/10.1186/s43043-024-00198-2","url":null,"abstract":"This bibliometric study aims to examine the associations of journals in the field of human reproduction with their access types and article processing charges to evaluate the evolving landscape of publishing in human reproduction. The primary databases, including Clarivate Analytics Master Journal List, Scopus®, PubMed, and Directory of Open Access Journals, were scrutinized to identify pertinent journals within the realm of human reproduction, utilizing keywords such as reproductive, reproduction, fertility, and infertility. Journals were excluded if they were not actively publishing in English or primarily focused on reproductive health, men’s health, sexual medicine, embryogenesis, developmental biology, or veterinary medicine concerning animal reproduction. A thorough characterization of the journals was conducted, followed by a comparative analysis of citation metrics and article processing charges across various access models. Forty-one journals were included into the study. A significant increase in the proportion of gold and diamond open-access journals was observed, rising from 42% (13 out of 31) to 53.6% (22 out of 41) by 2023. Hybrid journals demonstrated superior citation metrics compared to diamond open-access journals. For hybrid journals, a statistically significant, moderately positive correlation was found between article processing charges and CiteScore (rs (27) = 0.515, p < .024). Conversely, no correlation was observed between article processing charges and CiteScore for gold open-access journals (rs (27) = 0.445, p = 0.147). The mean article processing charges for all hybrid and gold open-access journals were calculated as US $3032.88 ± 1108.514 (312 to 4430). Specifically, the mean article processing charges for hybrid journals (US $3617.4 ± 610.19) were significantly higher than those for gold open-access journals (US $1916.82 ± 988.32), with a difference of 1700.658 (95% CI: 1124.861–2276.455), t (30) = 6.032, and p < .0005. Hybrid journals in the field of human reproduction carry fees nearly twice as high as those of gold open-access journals. The charging policies of gold open-access journals, which are not contigent upon citation metrics, emphasize the importance of caution for both authors and funders.","PeriodicalId":18532,"journal":{"name":"Middle East Fertility Society Journal","volume":"75 1","pages":""},"PeriodicalIF":1.3,"publicationDate":"2024-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141568651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-09DOI: 10.1186/s43043-024-00197-3
Akbayan Turesheva
There is considerable global discourse on the impact of insufficient vitamin D levels, known for their immunosuppressive properties, on recurrent pregnancy loss. Vitamin D deficiency affects 35% to 80% of the population. Despite advancements in molecular genetics, the study of vitamin D receptor gene (VDR) polymorphisms remains crucial. This study examined the correlation between VDR polymorphisms and idiopathic recurrent pregnancy loss. A narrative literature review with a meta-analysis of 85 sources from databases such as PubMed, Web of Science, and Scopus was conducted, focusing on studies from 2020 to 2022. The analysis included studies on vitamin D and miscarriage, adhering to ICD-10 criteria, and VDR gene allele analysis through PCR-RFLP. A comprehensive narrative analysis of the available scientific literature verified the link between comorbidities and vitamin D deficiencies, which can lead to recurrent pregnancy loss by hindering adaptive mechanisms and exacerbating complications. The most researched VDR gene polymorphisms, including FokI (rs2228570), BsmI (rs1544410), ApaI (rs7975232), TaqI (rs731236), and Cdx2, are linked to various health issues, particularly reproductive outcomes. The FokI (rs2228570) polymorphism in the VDR gene is a critical predictor of vitamin D levels, influencing pregnancy success. These findings are essential for assessing the risk of idiopathic recurrent pregnancy loss and developing new prevention and treatment approaches.
众所周知，维生素 D 具有免疫抑制作用，而维生素 D 含量不足会影响再次妊娠。35% 至 80% 的人口缺乏维生素 D。尽管分子遗传学取得了进步，但对维生素 D 受体基因（VDR）多态性的研究仍然至关重要。本研究探讨了 VDR 多态性与特发性复发性妊娠失败之间的相关性。研究人员对来自 PubMed、Web of Science 和 Scopus 等数据库的 85 篇文献进行了叙述性文献综述和荟萃分析，重点关注 2020 年至 2022 年的研究。分析包括有关维生素 D 和流产的研究，符合 ICD-10 标准，以及通过 PCR-RFLP 进行的 VDR 基因等位基因分析。对现有科学文献的综合叙述分析证实了合并症与维生素D缺乏之间的联系，维生素D缺乏会阻碍适应机制并加剧并发症，从而导致反复妊娠流产。研究最多的 VDR 基因多态性包括 FokI (rs2228570)、BsmI (rs1544410)、ApaI (rs7975232)、TaqI (rs731236) 和 Cdx2，这些基因多态性与各种健康问题，尤其是生殖结果有关。VDR 基因中的 FokI（rs2228570）多态性是维生素 D 水平的关键预测因子，会影响怀孕的成功率。这些发现对于评估特发性复发性妊娠失败的风险以及开发新的预防和治疗方法至关重要。
{"title":"Evaluation of the association of polymorphisms of the vitamin D receptor gene (VDR) with idiopathic recurrent pregnancy loss among women in Kazakhstan","authors":"Akbayan Turesheva","doi":"10.1186/s43043-024-00197-3","DOIUrl":"https://doi.org/10.1186/s43043-024-00197-3","url":null,"abstract":"There is considerable global discourse on the impact of insufficient vitamin D levels, known for their immunosuppressive properties, on recurrent pregnancy loss. Vitamin D deficiency affects 35% to 80% of the population. Despite advancements in molecular genetics, the study of vitamin D receptor gene (VDR) polymorphisms remains crucial. This study examined the correlation between VDR polymorphisms and idiopathic recurrent pregnancy loss. A narrative literature review with a meta-analysis of 85 sources from databases such as PubMed, Web of Science, and Scopus was conducted, focusing on studies from 2020 to 2022. The analysis included studies on vitamin D and miscarriage, adhering to ICD-10 criteria, and VDR gene allele analysis through PCR-RFLP. A comprehensive narrative analysis of the available scientific literature verified the link between comorbidities and vitamin D deficiencies, which can lead to recurrent pregnancy loss by hindering adaptive mechanisms and exacerbating complications. The most researched VDR gene polymorphisms, including FokI (rs2228570), BsmI (rs1544410), ApaI (rs7975232), TaqI (rs731236), and Cdx2, are linked to various health issues, particularly reproductive outcomes. The FokI (rs2228570) polymorphism in the VDR gene is a critical predictor of vitamin D levels, influencing pregnancy success. These findings are essential for assessing the risk of idiopathic recurrent pregnancy loss and developing new prevention and treatment approaches.","PeriodicalId":18532,"journal":{"name":"Middle East Fertility Society Journal","volume":"16 1","pages":""},"PeriodicalIF":1.3,"publicationDate":"2024-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141568753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-06DOI: 10.1186/s43043-024-00194-6
Solmaz Gul Sajjad, Aarush Mohammad Sajjad, Michael Fakih, Muhammad Ahsan Akhtar, Yasmin Sajjad
Evaluate the impact of SARS-CoV-2 on male fertility. Comprehensive analysis of studies exploring virus presence, inflammation, and altered semen parameters. While some studies report absence of SARS-CoV-2 in semen, others highlight testicular injury, inflammation, and potential viral orchitis. Scrotal discomfort and altered sperm parameters indicate reproductive implications. SARS-CoV-2 may pose significant challenges to male fertility, necessitating further research for a comprehensive understanding of its long-term effects. Limitations include varied testing methods and sample sizes, emphasizing the need for confirmation and detailed post-COVID examinations.
{"title":"Impact of SARS-CoV-2 on male reproductive system and fertility","authors":"Solmaz Gul Sajjad, Aarush Mohammad Sajjad, Michael Fakih, Muhammad Ahsan Akhtar, Yasmin Sajjad","doi":"10.1186/s43043-024-00194-6","DOIUrl":"https://doi.org/10.1186/s43043-024-00194-6","url":null,"abstract":"Evaluate the impact of SARS-CoV-2 on male fertility. Comprehensive analysis of studies exploring virus presence, inflammation, and altered semen parameters. While some studies report absence of SARS-CoV-2 in semen, others highlight testicular injury, inflammation, and potential viral orchitis. Scrotal discomfort and altered sperm parameters indicate reproductive implications. SARS-CoV-2 may pose significant challenges to male fertility, necessitating further research for a comprehensive understanding of its long-term effects. Limitations include varied testing methods and sample sizes, emphasizing the need for confirmation and detailed post-COVID examinations.","PeriodicalId":18532,"journal":{"name":"Middle East Fertility Society Journal","volume":"15 1","pages":""},"PeriodicalIF":1.3,"publicationDate":"2024-07-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141568745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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