Pub Date : 2024-03-01DOI: 10.1186/s43043-024-00173-x
Intan Kusumaningtyas, Djaswadi Dasuki, Sofia Mubarika Harjana, Ahmad Hamim Sadewa, Margaretha Cempaka Sweety, Linda Septiani
Folliculogenesis is an intricate process that involves the development and maturation of ovarian follicles in females. During folliculogenesis, multiple factors including hormones, growth factors, and signaling pathways regulate the growth and maturation of follicles. In recent years, microRNA, short non-coding RNA molecules, has gained attention due to its roles in the physiology and pathophysiology of various diseases in humans. It is known to have an important part in ovarian health and illness and its functions extend to several cellular processes. In this overview, we look at the importance of microRNAs in ovarian illnesses and how they function during follicle growth in the ovaries. Short RNA molecules (22 nucleotides) called microRNAs may influence several mRNA targets in different biological processes. The expression patterns of these small non-coding RNAs undergo dynamic changes during the several phases of follicular development; they play a function in post-transcriptional gene regulation. Follicle development, follicular atresia (regression of the follicles), and ovulation are all intricately regulated by the dynamic expression of distinct miRNAs throughout the various phases of folliculogenesis. The role of microRNAs (miRNAs), which are known to regulate gene expression, has recently come to light as crucial in the development and advancement of a number of ovarian diseases. Abnormalities of the human ovary, such as ovarian cancer, polycystic ovary syndrome (PCOS), and endometriosis, have prompted extensive research into the dysregulation of microRNAs. Endometriosis is associated with miRNAs that are known to have a role in processes such as invasion, cell growth, cell adhesion, angiogenesis, and epithelial-mesenchymal transition. The disturbance of target gene expression resulting from abnormal miRNA production is a potential factor contributing to cancer development. Some microRNAs (miRNAs) differ in expression levels between women with polycystic ovary syndrome and healthy controls, indicating that miRNAs may play a role in the development of PCOS. Extensive research carried out over the last 20 years has illuminated the roles of microRNAs (miRNAs), demonstrating their critical importance in controlling gene expression and the cell cycle. Changes in the quantities of microRNAs (miRNAs) may affect the aggressiveness of cancer and contribute to a variety of gynecological disorders. It appears that microRNAs hold potential as diagnostic biomarkers and treatment potential for various ovarian diseases.
{"title":"Unraveling the microRNAs, key players in folliculogenesis and ovarian diseases","authors":"Intan Kusumaningtyas, Djaswadi Dasuki, Sofia Mubarika Harjana, Ahmad Hamim Sadewa, Margaretha Cempaka Sweety, Linda Septiani","doi":"10.1186/s43043-024-00173-x","DOIUrl":"https://doi.org/10.1186/s43043-024-00173-x","url":null,"abstract":"Folliculogenesis is an intricate process that involves the development and maturation of ovarian follicles in females. During folliculogenesis, multiple factors including hormones, growth factors, and signaling pathways regulate the growth and maturation of follicles. In recent years, microRNA, short non-coding RNA molecules, has gained attention due to its roles in the physiology and pathophysiology of various diseases in humans. It is known to have an important part in ovarian health and illness and its functions extend to several cellular processes. In this overview, we look at the importance of microRNAs in ovarian illnesses and how they function during follicle growth in the ovaries. Short RNA molecules (22 nucleotides) called microRNAs may influence several mRNA targets in different biological processes. The expression patterns of these small non-coding RNAs undergo dynamic changes during the several phases of follicular development; they play a function in post-transcriptional gene regulation. Follicle development, follicular atresia (regression of the follicles), and ovulation are all intricately regulated by the dynamic expression of distinct miRNAs throughout the various phases of folliculogenesis. The role of microRNAs (miRNAs), which are known to regulate gene expression, has recently come to light as crucial in the development and advancement of a number of ovarian diseases. Abnormalities of the human ovary, such as ovarian cancer, polycystic ovary syndrome (PCOS), and endometriosis, have prompted extensive research into the dysregulation of microRNAs. Endometriosis is associated with miRNAs that are known to have a role in processes such as invasion, cell growth, cell adhesion, angiogenesis, and epithelial-mesenchymal transition. The disturbance of target gene expression resulting from abnormal miRNA production is a potential factor contributing to cancer development. Some microRNAs (miRNAs) differ in expression levels between women with polycystic ovary syndrome and healthy controls, indicating that miRNAs may play a role in the development of PCOS. Extensive research carried out over the last 20 years has illuminated the roles of microRNAs (miRNAs), demonstrating their critical importance in controlling gene expression and the cell cycle. Changes in the quantities of microRNAs (miRNAs) may affect the aggressiveness of cancer and contribute to a variety of gynecological disorders. It appears that microRNAs hold potential as diagnostic biomarkers and treatment potential for various ovarian diseases.","PeriodicalId":18532,"journal":{"name":"Middle East Fertility Society Journal","volume":"12 1","pages":""},"PeriodicalIF":1.3,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140019574","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-02-23DOI: 10.1186/s43043-024-00172-y
Anastasia A. Salame, Elias M. Dahdouh, Rania Aljafari, David A. Samuel, Bhavya P. Koodathingal, Aparna Bajpai, Shabin Kainoth, Michael Fakih
PGT-A has become an important part of IVF treatments. Despite its increased use, there are contradicting results on its role in improving reproductive outcomes of ART cycles. Given that aneuploidy is a main limiting factor for IVF success, we aimed to study the predictive factors of aneuploidy in infertile patients undergoing IVF and hence highlight the patients who would benefit the most from genetic testing. A retrospective analysis of 1242 blastocysts biopsied in the setting of PGT-A cycles was performed. The euploid group included 703 embryos, while the aneuploid group had 539 embryos. The factors included in the analyses were the couple’s history as well as the embryo characteristics. The primary outcome was the rate of aneuploid embryos per patient’s history as well as per embryo characteristics. The aneuploidy rate (AR) in our cohort was 43.4%. The woman’s age was found to be a significant predictor (OR 1.045, 95% CI 1.008–1.084, p = 0.016). Biopsy on day 5 as well as degree of expansion 3 was also found to affect significantly (OR 0.724, 95% CI .541–.970, p = 0.03 and OR 2.645, 95% CI 1.252–5.585, p = 0.011). Lack of consanguinity decreased the AR by an OR 0.274 with 95% CI .137–.547, p < 0.001. The number of blastocysts available, trophectoderm quality, embryo grade, gonadotropins as well as trigger used were not found to be significant predictors (p = 0.495, 0.649, 0.264, 0.717 and 0.659 respectively). Advanced female age, consanguinity, the day of embryo biopsy, and the degree of blastocyst expansion were all found to affect the incidence of AR. The age of the male partner, cause of infertility, and grade of embryo at biopsy were not found to correlate with aneuploidy.
PGT-A 已成为试管婴儿治疗的重要组成部分。尽管PGT-A的使用越来越多，但其在改善ART周期生殖结果方面的作用却存在着相互矛盾的结果。鉴于非整倍体是试管婴儿成功率的主要限制因素，我们旨在研究接受试管婴儿治疗的不孕患者非整倍体的预测因素，从而突出基因检测的最大受益者。我们对在 PGT-A 周期中活检的 1242 个囊胚进行了回顾性分析。优倍体组包括 703 个胚胎，而非整倍体组有 539 个胚胎。分析的因素包括夫妇的病史和胚胎特征。主要结果是根据患者病史和胚胎特征得出的非整倍体胚胎率。我们队列中的非整倍体率（AR）为 43.4%。妇女的年龄是一个重要的预测因素（OR 1.045，95% CI 1.008-1.084，p = 0.016）。第 5 天的活组织检查和扩张程度 3 也有显著影响（OR 0.724，95% CI .541-.970，p = 0.03 和 OR 2.645，95% CI 1.252-5.585，p = 0.011）。缺乏血缘关系会降低 AR，OR 值为 0.274，95% CI 为 .137-.547，p < 0.001。可用囊胚数量、滋养层质量、胚胎等级、促性腺激素以及使用的触发器都不是重要的预测因素（p = 0.495、0.649、0.264、0.717 和 0.659）。高龄女性、近亲结婚、胚胎活检日和囊胚扩张程度都会影响 AR 的发生率。男性伴侣的年龄、不孕原因和活检时胚胎的等级与非整倍体没有关联。
{"title":"Predictive factors of aneuploidy in infertile patients undergoing IVF: a retrospective analysis in a private IVF practice","authors":"Anastasia A. Salame, Elias M. Dahdouh, Rania Aljafari, David A. Samuel, Bhavya P. Koodathingal, Aparna Bajpai, Shabin Kainoth, Michael Fakih","doi":"10.1186/s43043-024-00172-y","DOIUrl":"https://doi.org/10.1186/s43043-024-00172-y","url":null,"abstract":"PGT-A has become an important part of IVF treatments. Despite its increased use, there are contradicting results on its role in improving reproductive outcomes of ART cycles. Given that aneuploidy is a main limiting factor for IVF success, we aimed to study the predictive factors of aneuploidy in infertile patients undergoing IVF and hence highlight the patients who would benefit the most from genetic testing. A retrospective analysis of 1242 blastocysts biopsied in the setting of PGT-A cycles was performed. The euploid group included 703 embryos, while the aneuploid group had 539 embryos. The factors included in the analyses were the couple’s history as well as the embryo characteristics. The primary outcome was the rate of aneuploid embryos per patient’s history as well as per embryo characteristics. The aneuploidy rate (AR) in our cohort was 43.4%. The woman’s age was found to be a significant predictor (OR 1.045, 95% CI 1.008–1.084, p = 0.016). Biopsy on day 5 as well as degree of expansion 3 was also found to affect significantly (OR 0.724, 95% CI .541–.970, p = 0.03 and OR 2.645, 95% CI 1.252–5.585, p = 0.011). Lack of consanguinity decreased the AR by an OR 0.274 with 95% CI .137–.547, p < 0.001. The number of blastocysts available, trophectoderm quality, embryo grade, gonadotropins as well as trigger used were not found to be significant predictors (p = 0.495, 0.649, 0.264, 0.717 and 0.659 respectively). Advanced female age, consanguinity, the day of embryo biopsy, and the degree of blastocyst expansion were all found to affect the incidence of AR. The age of the male partner, cause of infertility, and grade of embryo at biopsy were not found to correlate with aneuploidy.","PeriodicalId":18532,"journal":{"name":"Middle East Fertility Society Journal","volume":"6 1","pages":""},"PeriodicalIF":1.3,"publicationDate":"2024-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139947793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-02-12DOI: 10.1186/s43043-024-00168-8
Ngima Yangji Sherpa, Ahmed El Minawi, Ahmed N Askalany, Marwa Abdalla
Increased surgical efficacy has led to a remarkable increase in the usage of minimally invasive surgical procedures since their inception. The use of simulation in surgical teaching has grown significantly during the past 10 years. Several laparoscopic simulators have been built. Virtual reality (VR) simulators and box trainers (BTs), often known as pelvic trainers, are the two primary training modalities used in hospitals and clinical training institutes for the development and acquisition of laparoscopic skills. Our study aimed to evaluate the proper sequence of pelvic trainers and VR simulator training to improve laparoscopic gynecological skills. We carried out this pilot study at the Virtual Endoscopic Simulation and Skills Acquisition Laboratory at the Obstetrics and Gynecology Department in the Kasr Al Ainy Hospital, Faculty of Medicine, Cairo University, Egypt, from February to August 2022. All residents with minimal or without laparoscopic experience (twenty residents) were divided into two groups and classified as (group A versus group B). Group A’s training began with a pelvic trainer, which was tested using a checklist. Later, the group trained on a virtual reality simulator, which tested them using an electronic autoassessment. After training on a virtual reality simulator and passing an electronic autoassessment test, group B moved on to pelvic trainers and had a checklist-based assessment. We compared pelvic trainer tasks between the training groups, and detected no significant differences in camera navigation, cutting pattern, peg transfer, or running stitches (P values 0.646, 0.341, 0.179, and 0.939 respectively); when we compared VR simulator tasks between the training groups, there were no significant differences in camera navigation, cutting pattern, peg transfer, or running stitches (P values 0.79, 0.3, 0.33, and 0.06, respectively). There was no difference in training, between residents who started on a pelvic trainer or the VR simulator; therefore, both could be used in laparoscopic training with no preferred order. The trial was registered at clinicaltrials.gov with the name “Pelvic trainer vs VRS” and the identifier “NCT05255614.” The registration date was January 19, 2022, and the trial was prospectively registered. URL: https://register.clinicaltrials.gov/prs/app/action/ViewOrUnrelease?uid=U0004GED&ts=22&sid=S000BR5D&cx=t6mc14
{"title":"Comparative study to determine the proper sequence of simulation training, pelvic trainer versus virtual reality simulator: a pilot study","authors":"Ngima Yangji Sherpa, Ahmed El Minawi, Ahmed N Askalany, Marwa Abdalla","doi":"10.1186/s43043-024-00168-8","DOIUrl":"https://doi.org/10.1186/s43043-024-00168-8","url":null,"abstract":"Increased surgical efficacy has led to a remarkable increase in the usage of minimally invasive surgical procedures since their inception. The use of simulation in surgical teaching has grown significantly during the past 10 years. Several laparoscopic simulators have been built. Virtual reality (VR) simulators and box trainers (BTs), often known as pelvic trainers, are the two primary training modalities used in hospitals and clinical training institutes for the development and acquisition of laparoscopic skills. Our study aimed to evaluate the proper sequence of pelvic trainers and VR simulator training to improve laparoscopic gynecological skills. We carried out this pilot study at the Virtual Endoscopic Simulation and Skills Acquisition Laboratory at the Obstetrics and Gynecology Department in the Kasr Al Ainy Hospital, Faculty of Medicine, Cairo University, Egypt, from February to August 2022. All residents with minimal or without laparoscopic experience (twenty residents) were divided into two groups and classified as (group A versus group B). Group A’s training began with a pelvic trainer, which was tested using a checklist. Later, the group trained on a virtual reality simulator, which tested them using an electronic autoassessment. After training on a virtual reality simulator and passing an electronic autoassessment test, group B moved on to pelvic trainers and had a checklist-based assessment. We compared pelvic trainer tasks between the training groups, and detected no significant differences in camera navigation, cutting pattern, peg transfer, or running stitches (P values 0.646, 0.341, 0.179, and 0.939 respectively); when we compared VR simulator tasks between the training groups, there were no significant differences in camera navigation, cutting pattern, peg transfer, or running stitches (P values 0.79, 0.3, 0.33, and 0.06, respectively). There was no difference in training, between residents who started on a pelvic trainer or the VR simulator; therefore, both could be used in laparoscopic training with no preferred order. The trial was registered at clinicaltrials.gov with the name “Pelvic trainer vs VRS” and the identifier “NCT05255614.” The registration date was January 19, 2022, and the trial was prospectively registered. URL: https://register.clinicaltrials.gov/prs/app/action/ViewOrUnrelease?uid=U0004GED&ts=22&sid=S000BR5D&cx=t6mc14 ","PeriodicalId":18532,"journal":{"name":"Middle East Fertility Society Journal","volume":"22 1","pages":""},"PeriodicalIF":1.3,"publicationDate":"2024-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139757398","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-30DOI: 10.1186/s43043-024-00169-7
Essam R. Othman, Ahmed M. Abdelmagied, Maha Y. Khashbah, Cornelis B. Lambalk, Velja Mijatovic
Firstly, to measure indicators of health-related quality of life (HRQoL) in Egyptian women with endometriosis; and secondly, to estimate time interval from start of symptoms until endometriosis diagnosis is made (diagnostic delay) in Egyptian women with the disease. Before laparoscopy for pelvic pain and/or infertility, eligible Egyptian women completed Global Study of Women’s Health (GSWH) questionnaire and validated Arabic version of Rand SF 36 (SF-36). According to laparoscopic findings, participants were divided to endometriosis group and control women with no pelvic abnormalities. Seventy women with endometriosis and 57 symptomatic controls without endometriosis were enrolled. A diagnostic delay of 36 months (IQR 22.5–60) was observed in women with endometriosis while symptomatic controls had a delay of 48 months (IQR 24–84). The difference was not statistically significant (P = 0.08). Bodily pain (BP) scores were significantly lower in women with endometriosis than controls [80.0 (45.0–100.0) versus 100.0 (68.75–100.0) respectively, P is 0.01]. Women with advanced endometriosis had significantly lower scores for physical functioning (PF), role limitation due to physical function (RP), and BP compared to women with mild endometriosis, and to controls. Physical component summary (PCS) scores were significantly lower in women with advanced stage endometriosis [41.51 (34.19–51.54] compared to women with early-stage disease [58.33 (50.98–60.37)] or control group [54.72 (48.81–59.58)]. Patient’s age, intensity of noncyclical pelvic pain, and disease stage are determining factors of HRQoL in women with endometriosis. Egyptian women with endometriosis experience relatively short diagnostic delay, poor bodily pain scores, and impaired physical health for which age, disease stage, and non-cyclic pain are determinants. Multi-disciplinary endometriosis centers, educational programs, and patient support groups are needed in Egypt.
{"title":"Diagnostic delay and health-related quality of life in Egyptian women with endometriosis","authors":"Essam R. Othman, Ahmed M. Abdelmagied, Maha Y. Khashbah, Cornelis B. Lambalk, Velja Mijatovic","doi":"10.1186/s43043-024-00169-7","DOIUrl":"https://doi.org/10.1186/s43043-024-00169-7","url":null,"abstract":"Firstly, to measure indicators of health-related quality of life (HRQoL) in Egyptian women with endometriosis; and secondly, to estimate time interval from start of symptoms until endometriosis diagnosis is made (diagnostic delay) in Egyptian women with the disease. Before laparoscopy for pelvic pain and/or infertility, eligible Egyptian women completed Global Study of Women’s Health (GSWH) questionnaire and validated Arabic version of Rand SF 36 (SF-36). According to laparoscopic findings, participants were divided to endometriosis group and control women with no pelvic abnormalities. Seventy women with endometriosis and 57 symptomatic controls without endometriosis were enrolled. A diagnostic delay of 36 months (IQR 22.5–60) was observed in women with endometriosis while symptomatic controls had a delay of 48 months (IQR 24–84). The difference was not statistically significant (P = 0.08). Bodily pain (BP) scores were significantly lower in women with endometriosis than controls [80.0 (45.0–100.0) versus 100.0 (68.75–100.0) respectively, P is 0.01]. Women with advanced endometriosis had significantly lower scores for physical functioning (PF), role limitation due to physical function (RP), and BP compared to women with mild endometriosis, and to controls. Physical component summary (PCS) scores were significantly lower in women with advanced stage endometriosis [41.51 (34.19–51.54] compared to women with early-stage disease [58.33 (50.98–60.37)] or control group [54.72 (48.81–59.58)]. Patient’s age, intensity of noncyclical pelvic pain, and disease stage are determining factors of HRQoL in women with endometriosis. Egyptian women with endometriosis experience relatively short diagnostic delay, poor bodily pain scores, and impaired physical health for which age, disease stage, and non-cyclic pain are determinants. Multi-disciplinary endometriosis centers, educational programs, and patient support groups are needed in Egypt.","PeriodicalId":18532,"journal":{"name":"Middle East Fertility Society Journal","volume":"63 1","pages":""},"PeriodicalIF":1.3,"publicationDate":"2024-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139579370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-26DOI: 10.1186/s43043-024-00170-0
Raminder Kaur, Maninder Kaur
Polycystic ovary syndrome is an escalating endocrinal and reproductive disorder among women of reproductive age and is considered the foremost health burden. Therefore, the present study is an attempt to estimate the potential association of metabolic syndrome in obese and non-obese PCOS women. A total of 250 PCOS women ranging in age from 18 to 45 years and living in the Chandigarh Capital Region were selected from the OPD, PGIMER, Chandigarh, (North India). Rotterdam Criteria (2003) was employed to diagnose polycystic ovary syndrome among women. The prevalence of metabolic syndrome (MS) as assessed by the National Cholesterol Education Program Adult Treatment Panel (NCEP ATP III) was found to be 30% (4% in non-obese and 26% in obese) in PCOS women. Waist circumference > 88 cm is the most prevalent feature in PCOS women (67.2%) followed by HDL < 50 mg/dL (56.8%). The multivariate logistic regression analysis exhibited that visceral adiposity index (VAI) was a significant determinant of metabolic syndrome in obese (OR = 1.17, p < 0.01) and non-obese (OR = 1.2, p < 0.01) PCOS women. Results of ROC analysis further established visceral adiposity index as a potential determinant in metabolic syndrome in both the groups of PCOS women. In obese PCOS women, the W/H ratio also depicted accuracy in predicting metabolic syndrome risk. Visceral adiposity index is a significant tool to assess the metabolic syndrome in both groups, i.e., obese and non-obese. However, the waist/hip ratio can be considered as a predictive tool in obese women only. Thus, it could be used as a significant and inexpensive tool in clinical practices for early detection of metabolic syndrome in PCOS women.
{"title":"Evaluation of potential association of metabolic syndrome in obese and non-obese PCOS women","authors":"Raminder Kaur, Maninder Kaur","doi":"10.1186/s43043-024-00170-0","DOIUrl":"https://doi.org/10.1186/s43043-024-00170-0","url":null,"abstract":"Polycystic ovary syndrome is an escalating endocrinal and reproductive disorder among women of reproductive age and is considered the foremost health burden. Therefore, the present study is an attempt to estimate the potential association of metabolic syndrome in obese and non-obese PCOS women. A total of 250 PCOS women ranging in age from 18 to 45 years and living in the Chandigarh Capital Region were selected from the OPD, PGIMER, Chandigarh, (North India). Rotterdam Criteria (2003) was employed to diagnose polycystic ovary syndrome among women. The prevalence of metabolic syndrome (MS) as assessed by the National Cholesterol Education Program Adult Treatment Panel (NCEP ATP III) was found to be 30% (4% in non-obese and 26% in obese) in PCOS women. Waist circumference > 88 cm is the most prevalent feature in PCOS women (67.2%) followed by HDL < 50 mg/dL (56.8%). The multivariate logistic regression analysis exhibited that visceral adiposity index (VAI) was a significant determinant of metabolic syndrome in obese (OR = 1.17, p < 0.01) and non-obese (OR = 1.2, p < 0.01) PCOS women. Results of ROC analysis further established visceral adiposity index as a potential determinant in metabolic syndrome in both the groups of PCOS women. In obese PCOS women, the W/H ratio also depicted accuracy in predicting metabolic syndrome risk. Visceral adiposity index is a significant tool to assess the metabolic syndrome in both groups, i.e., obese and non-obese. However, the waist/hip ratio can be considered as a predictive tool in obese women only. Thus, it could be used as a significant and inexpensive tool in clinical practices for early detection of metabolic syndrome in PCOS women.","PeriodicalId":18532,"journal":{"name":"Middle East Fertility Society Journal","volume":"11 1","pages":""},"PeriodicalIF":1.3,"publicationDate":"2024-01-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139590561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-20DOI: 10.1186/s43043-024-00167-9
Nearmeen M. Rashad, Walid Mohamed Elnagar, Dina Rasheed Issa, Marwa H. S. Hussien, Rehab M. Atef, Hoda Afifi
Polycystic ovary syndrome (PCOS) is known as the most common endocrine/metabolic disorder in women of reproductive age. Long non-coding RNAs (lncRNAs) regulate a wide range of physiological and pathological processes. We designed this study to evaluate lncRNA H19 relative expression in patients with PCOS and to evaluate its impact on metabolic features and reproductive phenotypes of Egyptian women with polycystic ovaries. The case–control study enrolled 50 control groups and 50 patients, with PCOS. The selection of patients with PCOS depended on the diagnosis according to the Rotterdam Consensus (2004). The lncRNA H19 were measured by real-time quantitative polymerase chain reaction (RT-qPCR). The lncRNA H19 level was significantly higher in the PCOS group (1.71 ± 0.48) compared to controls (0.924 ± 0.081). Furthermore, lncRNA H19 levels were significantly positively correlated with anthropometric and metabolic parameters including BMI, waist/hip ratio, TC, TG, LDL, FPG, FSI, HbA1c, and HOMA-IR. Regarding reproductive phenotypes features, hirsutism score, and AFC levels were significantly positively correlated with lncRNA H19 levels. The linear regression test revealed that BMI and AFC were the only parameters independently associated with lncRNA H19 among other studied parameters. Interestingly, receiver operating characteristic curve (ROC) analysis detected that the area under the curve (AUC) for the lncRNA H19 was 0.925 (95% CI = 0.856–0.955) with sensitivity = 96.4%, specificity = 96%, and the cutoff values (1.08). Thus, the predictive power of lncRNA H19 of PCOS was highly sensitive and specific. PCOS patients had significantly higher lncRNA H19 levels than controls. lncRNA H19 levels were significantly positively correlated with metabolic risk factors as well as clinical and laboratory features of PCOS.
{"title":"The impact of long non-coding RNA H19 on metabolic features and reproductive phenotypes of Egyptian women with polycystic ovary syndrome","authors":"Nearmeen M. Rashad, Walid Mohamed Elnagar, Dina Rasheed Issa, Marwa H. S. Hussien, Rehab M. Atef, Hoda Afifi","doi":"10.1186/s43043-024-00167-9","DOIUrl":"https://doi.org/10.1186/s43043-024-00167-9","url":null,"abstract":"Polycystic ovary syndrome (PCOS) is known as the most common endocrine/metabolic disorder in women of reproductive age. Long non-coding RNAs (lncRNAs) regulate a wide range of physiological and pathological processes. We designed this study to evaluate lncRNA H19 relative expression in patients with PCOS and to evaluate its impact on metabolic features and reproductive phenotypes of Egyptian women with polycystic ovaries. The case–control study enrolled 50 control groups and 50 patients, with PCOS. The selection of patients with PCOS depended on the diagnosis according to the Rotterdam Consensus (2004). The lncRNA H19 were measured by real-time quantitative polymerase chain reaction (RT-qPCR). The lncRNA H19 level was significantly higher in the PCOS group (1.71 ± 0.48) compared to controls (0.924 ± 0.081). Furthermore, lncRNA H19 levels were significantly positively correlated with anthropometric and metabolic parameters including BMI, waist/hip ratio, TC, TG, LDL, FPG, FSI, HbA1c, and HOMA-IR. Regarding reproductive phenotypes features, hirsutism score, and AFC levels were significantly positively correlated with lncRNA H19 levels. The linear regression test revealed that BMI and AFC were the only parameters independently associated with lncRNA H19 among other studied parameters. Interestingly, receiver operating characteristic curve (ROC) analysis detected that the area under the curve (AUC) for the lncRNA H19 was 0.925 (95% CI = 0.856–0.955) with sensitivity = 96.4%, specificity = 96%, and the cutoff values (1.08). Thus, the predictive power of lncRNA H19 of PCOS was highly sensitive and specific. PCOS patients had significantly higher lncRNA H19 levels than controls. lncRNA H19 levels were significantly positively correlated with metabolic risk factors as well as clinical and laboratory features of PCOS.","PeriodicalId":18532,"journal":{"name":"Middle East Fertility Society Journal","volume":"7 1","pages":""},"PeriodicalIF":1.3,"publicationDate":"2024-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139508205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-20DOI: 10.1186/s43043-024-00166-w
Hassan Maghraby, Hesham Saleh, Ismail L. Fourtia, Salah Rasheed, Mohamed Elmahdy, Amr S. Abdelbadie, Federica Di Guardo, Panagiotis Drakopoulos, Habib Midassi, Ahmed Shoukry
Triggering final oocyte maturation is a pivotal step in modern patient-tailored IVF/ICSI treatment, securing the optimal number of mature oocytes retrieved without compromising fertilization, embryo development, and live birth. Several factors need to be considered when deciding the time of the trigger: the size of the leading follicles, distribution of the follicular cohort, the duration of stimulation, the protocol used for stimulation, and ovarian response status. The current narrative review aims to appraise all available evidence for determining the proper time for inducing final oocyte maturation following IVF treatment. Moreover, it discusses the impact of the stimulation protocol, follicular size, and magnitude of ovarian response on choosing the proper timing for trigger. Comprehensive literature search of all available articles and relevant articles studying the criteria for timing of final oocyte maturation trigger in IVF/ICSI cycles were included in this review. It was found that leading follicles size of 16–22 mm is associated with the optimum oocyte maturation ratio, size of the remaining cohort of follicles should be ≥ 14 mm, 10–12 days of minimum length of stimulation should be auspicated in normal responders before trigger, and the timing of trigger administration should not depend solely on hormonal levels. In conclusion, the timing of triggering of final oocyte maturation in ICSI cycles should be individualized on a case-by-case basis.
触发最终卵母细胞成熟是现代试管婴儿/卵胞浆内单精子显微注射（IVF/ICSI）治疗中为患者量身定制的一个关键步骤，可确保在不影响受精、胚胎发育和活产的情况下获得最佳数量的成熟卵母细胞。在决定促排时间时需要考虑几个因素：领先卵泡的大小、卵泡群的分布、促排持续时间、促排方案以及卵巢反应状态。本综述旨在评估所有可用证据，以确定试管婴儿治疗后诱导最终卵母细胞成熟的适当时间。此外，它还讨论了刺激方案、卵泡大小和卵巢反应程度对选择合适的触发时机的影响。本综述全面检索了所有可用文献，并纳入了研究试管婴儿/卵胞浆内单精子显微注射周期中最终卵母细胞成熟触发时机标准的相关文章。结果发现，16-22 mm 的前导卵泡大小与最佳卵母细胞成熟比例相关，剩余卵泡群的大小应≥ 14 mm，正常反应者在触发前至少应接受 10-12 天的刺激，且触发时间不应仅取决于激素水平。总之，ICSI 周期中触发卵母细胞最终成熟的时机应根据具体情况而定。
{"title":"The dilemma of the trigger timing in IVF: a review","authors":"Hassan Maghraby, Hesham Saleh, Ismail L. Fourtia, Salah Rasheed, Mohamed Elmahdy, Amr S. Abdelbadie, Federica Di Guardo, Panagiotis Drakopoulos, Habib Midassi, Ahmed Shoukry","doi":"10.1186/s43043-024-00166-w","DOIUrl":"https://doi.org/10.1186/s43043-024-00166-w","url":null,"abstract":"Triggering final oocyte maturation is a pivotal step in modern patient-tailored IVF/ICSI treatment, securing the optimal number of mature oocytes retrieved without compromising fertilization, embryo development, and live birth. Several factors need to be considered when deciding the time of the trigger: the size of the leading follicles, distribution of the follicular cohort, the duration of stimulation, the protocol used for stimulation, and ovarian response status. The current narrative review aims to appraise all available evidence for determining the proper time for inducing final oocyte maturation following IVF treatment. Moreover, it discusses the impact of the stimulation protocol, follicular size, and magnitude of ovarian response on choosing the proper timing for trigger. Comprehensive literature search of all available articles and relevant articles studying the criteria for timing of final oocyte maturation trigger in IVF/ICSI cycles were included in this review. It was found that leading follicles size of 16–22 mm is associated with the optimum oocyte maturation ratio, size of the remaining cohort of follicles should be ≥ 14 mm, 10–12 days of minimum length of stimulation should be auspicated in normal responders before trigger, and the timing of trigger administration should not depend solely on hormonal levels. In conclusion, the timing of triggering of final oocyte maturation in ICSI cycles should be individualized on a case-by-case basis.","PeriodicalId":18532,"journal":{"name":"Middle East Fertility Society Journal","volume":"17 1","pages":""},"PeriodicalIF":1.3,"publicationDate":"2024-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139508114","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-17DOI: 10.1186/s43043-024-00164-y
A. Abdelmagied, Alaa A. Makhlouf, Ahmed A. Abdel-Aleem, Safwat A. Mohamed, Ahmed Nasr, Azza Abo Elfadl, Mohammed K. Ali
Our research question is could follicular fluid (FF) leptin solely or contemporaneously with other clinical, biochemical, and sonographic adiposity measures predict the probability of having a live birth during ICSI cycles? This is a prospective cohort study that enrolled infertile women without polycystic ovary syndrome scheduled for ICSI. At baseline, women had an assessment of obesity using different metrics: clinical, serum biochemical, and sonographic. Clinical measures encompassed waist circumference and body mass index. Biochemical evaluation comprised an assessment of the homeostasis model for insulin resistance, visceral adiposity index, and lipid accumulation product. Preperitoneal and subcutaneous abdominal fat were measured using ultrasound and body fat index was calculated. On the day of oocyte retrieval, pooled FF was sampled to assess FF leptin. Our primary outcome was live birth after one fresh embryo transfer cycle. Out of 91 women analyzed in this study, 28 have a live birth (30.8%). No difference in FF leptin concentration was found between women with and without live birth (mean ± SD; 20336 ± 8006 vs 18493 ± 6655 pg/ml; P = 0.2). None of the assessed adiposity markers was a predictor for live birth. Substantially, follicular fluid leptin was positively correlated with insulin resistance in women with and without live birth (r = 0.21, P = 0.04). In logistic regression analysis, the outcome of the prior cycle, the ability to have cryopreserved embryos, and the oocyte maturation index were the predictors for live birth in our study. The present work could not find evidence that follicular fluid leptin, preperitoneal fat, and other evaluated adiposity measures could impact live births after ICSI cycles.
{"title":"Revisiting the predictability of follicular fluid leptin and related adiposity measures for live birth in women scheduled for ICSI cycles: a prospective cohort study","authors":"A. Abdelmagied, Alaa A. Makhlouf, Ahmed A. Abdel-Aleem, Safwat A. Mohamed, Ahmed Nasr, Azza Abo Elfadl, Mohammed K. Ali","doi":"10.1186/s43043-024-00164-y","DOIUrl":"https://doi.org/10.1186/s43043-024-00164-y","url":null,"abstract":"Our research question is could follicular fluid (FF) leptin solely or contemporaneously with other clinical, biochemical, and sonographic adiposity measures predict the probability of having a live birth during ICSI cycles? This is a prospective cohort study that enrolled infertile women without polycystic ovary syndrome scheduled for ICSI. At baseline, women had an assessment of obesity using different metrics: clinical, serum biochemical, and sonographic. Clinical measures encompassed waist circumference and body mass index. Biochemical evaluation comprised an assessment of the homeostasis model for insulin resistance, visceral adiposity index, and lipid accumulation product. Preperitoneal and subcutaneous abdominal fat were measured using ultrasound and body fat index was calculated. On the day of oocyte retrieval, pooled FF was sampled to assess FF leptin. Our primary outcome was live birth after one fresh embryo transfer cycle. Out of 91 women analyzed in this study, 28 have a live birth (30.8%). No difference in FF leptin concentration was found between women with and without live birth (mean ± SD; 20336 ± 8006 vs 18493 ± 6655 pg/ml; P = 0.2). None of the assessed adiposity markers was a predictor for live birth. Substantially, follicular fluid leptin was positively correlated with insulin resistance in women with and without live birth (r = 0.21, P = 0.04). In logistic regression analysis, the outcome of the prior cycle, the ability to have cryopreserved embryos, and the oocyte maturation index were the predictors for live birth in our study. The present work could not find evidence that follicular fluid leptin, preperitoneal fat, and other evaluated adiposity measures could impact live births after ICSI cycles.","PeriodicalId":18532,"journal":{"name":"Middle East Fertility Society Journal","volume":"1 1","pages":""},"PeriodicalIF":1.3,"publicationDate":"2024-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139481100","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Second ejaculation can influence sperm quality which may define the first-line treatment. The purpose of this study was to evaluate the effectiveness of a second ejaculation in decreasing the unexpected intracytoplasmic sperm injection (ICSI) rate by a propensity score-matched (PSM) analysis. Patients who were projected to undergo IVF were included between January 2016 and November 2021 in this monocentric, retrospective analysis. 2782 patients included in the study, 143 and 2639 patients were non-randomized in the unexpected ICSI and IVF groups, respectively. One hundred fourteen patients with unexpected ICSI produced two semen samples on the day of ovum pick-up. After 1:4 PSM, we matched 61 patients in the second ejaculation IVF group to 238 patients in the conventional IVF group. Outcomes of sperm quality, fertilization rate, embryo quality, and pregnancy were compared. Second ejaculation significantly improved sperm concentration, progressive motility before and after sperm swim-up, total progressive motility sperm count after swim-up, and decreased sperm DNA fragmentation (SDF). Sixty-one of 114 (53.5%) unexpected ICSI couples had enough total progressive motility sperm for IVF with the second ejaculation. There were no differences in basic clinical characteristics between couples in second ejaculation IVF and matched-conventional IVF group. For the two groups, no differences were observed in IVF outcomes. However, a significant increase in good-quality blastocyst rate was observed for second-ejaculation IVF couples. Univariate and multivariate linear regression analysis also confirmed that the second ejaculation was an independent risk factor for the good quality blastocyst rate. Second ejaculation could be an economical and secure alternative to get good quality sperm, and blastocyst and decrease the rate of unexpected ICSI. Multicenter studies should be conducted to confirm the potential advantages of using second ejaculation IVF in effectively reducing the rate of ICSI.
{"title":"Second ejaculation produces good quality sperm and blastocyst and decreases the rate of unexpected ICSI cycle: a propensity score-matched analysis","authors":"Xiaohui Zhang, Shikai Wang, Yueyue Huang, Xianbao Mao, Zhengda Li, Pingpin Wei, Liangshi Chen, Dawen Li, Lintao Xue","doi":"10.1186/s43043-024-00165-x","DOIUrl":"https://doi.org/10.1186/s43043-024-00165-x","url":null,"abstract":"Second ejaculation can influence sperm quality which may define the first-line treatment. The purpose of this study was to evaluate the effectiveness of a second ejaculation in decreasing the unexpected intracytoplasmic sperm injection (ICSI) rate by a propensity score-matched (PSM) analysis. Patients who were projected to undergo IVF were included between January 2016 and November 2021 in this monocentric, retrospective analysis. 2782 patients included in the study, 143 and 2639 patients were non-randomized in the unexpected ICSI and IVF groups, respectively. One hundred fourteen patients with unexpected ICSI produced two semen samples on the day of ovum pick-up. After 1:4 PSM, we matched 61 patients in the second ejaculation IVF group to 238 patients in the conventional IVF group. Outcomes of sperm quality, fertilization rate, embryo quality, and pregnancy were compared. Second ejaculation significantly improved sperm concentration, progressive motility before and after sperm swim-up, total progressive motility sperm count after swim-up, and decreased sperm DNA fragmentation (SDF). Sixty-one of 114 (53.5%) unexpected ICSI couples had enough total progressive motility sperm for IVF with the second ejaculation. There were no differences in basic clinical characteristics between couples in second ejaculation IVF and matched-conventional IVF group. For the two groups, no differences were observed in IVF outcomes. However, a significant increase in good-quality blastocyst rate was observed for second-ejaculation IVF couples. Univariate and multivariate linear regression analysis also confirmed that the second ejaculation was an independent risk factor for the good quality blastocyst rate. Second ejaculation could be an economical and secure alternative to get good quality sperm, and blastocyst and decrease the rate of unexpected ICSI. Multicenter studies should be conducted to confirm the potential advantages of using second ejaculation IVF in effectively reducing the rate of ICSI.","PeriodicalId":18532,"journal":{"name":"Middle East Fertility Society Journal","volume":"105 1","pages":""},"PeriodicalIF":1.3,"publicationDate":"2024-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139396488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-02DOI: 10.1186/s43043-023-00160-8
Heba M. Mohamed, Treza S. Badia, Shimaa A. Khalaf, Saleh O. Abdullah, Tarek A. Farghaly, Ahmed N. Fetih, Shimaa Elwardany Aly
Stress and dissatisfaction are common issues among patients undergoing in vitro fertilization (IVF). The process of IVF can be emotionally and psychologically challenging, leading to stress, anxiety, and depression in patients. This can be exacerbated by factors such as the length of infertility, the uncertainty of the outcome, and the financial burden of treatment. Additionally, the physical demands of the treatment, the side effects of medication, and the fear of failure can contribute to dissatisfaction and emotional strain in IVF patients. This pre-post intervention study design aimed to evaluate the effect of a health education program on knowledge, stress, and satisfaction among 100 infertile women undergoing IVF attending the Woman’s Health Hospital’s infertility outpatient clinic and IVF unit at Assiut University. Knowledge and stress were measured by women’s knowledge about IVF and the perceived stress scale, respectively. The satisfaction was measured using the Scale of Patient Satisfaction about IVF. There is a marked improvement in the knowledge of infertile women, with a significant reduction in their stress levels after the health education program. Furthermore, the satisfaction scores of the women who participated in the program improved significantly. The health education program demonstrated a positive impact on knowledge, stress levels, and satisfaction among infertile women undergoing IVF. These findings highlight the positive impact of the program on the participants and emphasize the importance of continuous educational programs regarding IVF to improve knowledge, decrease stress levels, and increase their overall satisfaction with the treatment.
{"title":"Effect of health education program on knowledge, stress, and satisfaction among infertile women undergoing in vitro fertilization injection","authors":"Heba M. Mohamed, Treza S. Badia, Shimaa A. Khalaf, Saleh O. Abdullah, Tarek A. Farghaly, Ahmed N. Fetih, Shimaa Elwardany Aly","doi":"10.1186/s43043-023-00160-8","DOIUrl":"https://doi.org/10.1186/s43043-023-00160-8","url":null,"abstract":"Stress and dissatisfaction are common issues among patients undergoing in vitro fertilization (IVF). The process of IVF can be emotionally and psychologically challenging, leading to stress, anxiety, and depression in patients. This can be exacerbated by factors such as the length of infertility, the uncertainty of the outcome, and the financial burden of treatment. Additionally, the physical demands of the treatment, the side effects of medication, and the fear of failure can contribute to dissatisfaction and emotional strain in IVF patients. This pre-post intervention study design aimed to evaluate the effect of a health education program on knowledge, stress, and satisfaction among 100 infertile women undergoing IVF attending the Woman’s Health Hospital’s infertility outpatient clinic and IVF unit at Assiut University. Knowledge and stress were measured by women’s knowledge about IVF and the perceived stress scale, respectively. The satisfaction was measured using the Scale of Patient Satisfaction about IVF. There is a marked improvement in the knowledge of infertile women, with a significant reduction in their stress levels after the health education program. Furthermore, the satisfaction scores of the women who participated in the program improved significantly. The health education program demonstrated a positive impact on knowledge, stress levels, and satisfaction among infertile women undergoing IVF. These findings highlight the positive impact of the program on the participants and emphasize the importance of continuous educational programs regarding IVF to improve knowledge, decrease stress levels, and increase their overall satisfaction with the treatment.","PeriodicalId":18532,"journal":{"name":"Middle East Fertility Society Journal","volume":"33 1","pages":""},"PeriodicalIF":1.3,"publicationDate":"2024-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139078187","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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