Asmaa S. Sleem, Samar S. Salman, Wafaa A. Shehata, Amal M. Dawoud
Objectives: To assess glycerol antibacterial activity against both Gram positive and negative microorganisms, detect impact of increasing glycerol concentration in bacterial cultures on extra/ intracellular Glycerol-3-phosphate (G3P) concentration, evaluate antiseptic efficacy of different glycerol/alcohol formulations and monitor HCWs tolerability to these formulations to recommend the best antiseptic preparation in vitro. Background: The progressively rising nosocomial infections have enforced the need for novel infection prevention modalities. Glycerol emerges as a significant and cheap antibacterial agent against both Gram positive and negative organisms without any toxic effects. Methodology: Different glycerol/ethanol formulations were evaluated for both bacterial inhibitory and bactericidal activity against selected bacteria (4 reference and 5 clinical strains). Effect of glycerol/ethanol combination was tested by checkerboard method. Colorimetric assay of extra and intracellular bacterial concentration of G3P was done. Skin tolerance of involved HCWs was evaluated against obtained bactericidal formulations including; 80% ethanol (E80)without glycerol (G0) as group (A), E80G1.45 (B), E80G20 (C)and E0G80 as group (D). Results: Microbial growth inhibition was detected at G40E40, G40E60, G1.45 E80 and G20 E80 for most strains. No visible growth (bactericidal effect) was detected with G80, G20 E80 and G1.45 E80. Glycerol/ethanol combination showed synergistic effect against Enterococcus faecalis and biofilm producing MRSA with additive effect against Proteus mirabilis, staphylococcus aureus. Acinetobacter baumannii displayed indifference. No antagonism was detected. G3P levels were positively correlated with added glycerol concentration. E80G20 achieved HCWs maximum tolerability (100%) followed by E0G80 (92.3%). Conclusion: E80G20 and E0G80 were superior to WHO recommended E80G1.45. However, more advanced studies are needed.
{"title":"Evaluation of glycerol antiseptic effect on Gram positive and Gram negative bacteria","authors":"Asmaa S. Sleem, Samar S. Salman, Wafaa A. Shehata, Amal M. Dawoud","doi":"10.59204/2314-6788.1100","DOIUrl":"https://doi.org/10.59204/2314-6788.1100","url":null,"abstract":"Objectives: To assess glycerol antibacterial activity against both Gram positive and negative microorganisms, detect impact of increasing glycerol concentration in bacterial cultures on extra/ intracellular Glycerol-3-phosphate (G3P) concentration, evaluate antiseptic efficacy of different glycerol/alcohol formulations and monitor HCWs tolerability to these formulations to recommend the best antiseptic preparation in vitro. Background: The progressively rising nosocomial infections have enforced the need for novel infection prevention modalities. Glycerol emerges as a significant and cheap antibacterial agent against both Gram positive and negative organisms without any toxic effects. Methodology: Different glycerol/ethanol formulations were evaluated for both bacterial inhibitory and bactericidal activity against selected bacteria (4 reference and 5 clinical strains). Effect of glycerol/ethanol combination was tested by checkerboard method. Colorimetric assay of extra and intracellular bacterial concentration of G3P was done. Skin tolerance of involved HCWs was evaluated against obtained bactericidal formulations including; 80% ethanol (E80)without glycerol (G0) as group (A), E80G1.45 (B), E80G20 (C)and E0G80 as group (D). Results: Microbial growth inhibition was detected at G40E40, G40E60, G1.45 E80 and G20 E80 for most strains. No visible growth (bactericidal effect) was detected with G80, G20 E80 and G1.45 E80. Glycerol/ethanol combination showed synergistic effect against Enterococcus faecalis and biofilm producing MRSA with additive effect against Proteus mirabilis, staphylococcus aureus. Acinetobacter baumannii displayed indifference. No antagonism was detected. G3P levels were positively correlated with added glycerol concentration. E80G20 achieved HCWs maximum tolerability (100%) followed by E0G80 (92.3%). Conclusion: E80G20 and E0G80 were superior to WHO recommended E80G1.45. However, more advanced studies are needed.","PeriodicalId":18558,"journal":{"name":"Menoufia Medical Journal","volume":"301 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135483927","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Olfat M. Hendy, Dina A. Fouad, Shaimaa A. Pessar, Sara M. Aboagiza, Hanan M. Bedair
Objectives: To evaluate the integration of clinical, morphological, cytogenetic features and MPL gene mutation analysis in Egyptian patients with clonal thrombocytosis and correlate these finding to patient’s outcome. Background: Thrombocytosis can be caused by either reactive or autonomous processes as a result of genetic alterations that affect signaling pathway through gain-of-function activity of MPL (myeloproliferative leukemia virus), Janus kinase 2 (JAK 2), calreticulin (CALR). Methods: This study was conducted on 60 Egyptian patients having clonal thrombocytosis attending to clinical pathology department, Ain Shams University Hospitals during the period from August 2020 to December 2021. Complete blood count, LDH level and MPL gene mutation by Real-time PCR were measured. Results: JAK2 gene mutation was more prevalent than MPL gene mutation in our study (40% and 8.3% respectively). MPL mutation has an aggressive nature than JAK2 mutation as evident by; older age (68.80± 2.28 vs 55.5 ± 8.28 accordingly), more thrombotic manifestation (100% vs 58.3%), higher platelets count (1221.4 ± 309.70 vs 879.38 ± 215.98) and poor response to therapy (0% of MPL positive patients achieved complete response to therapy vs 41.7% for JAK2 positive patients) but with no marked morphological difference regarding megakaryocytes morphology. Conclusion: MPL W515L/K mutations present in 10.3% in essential thrombocythemia Egyptian patients, included in our current study. MPL gene mutation has a bad prognostic effect. Moreover, presence of coexisting JAK2 & MPL mutation have a synergistic bad effect to be evaluated in further larger studies.
目的:评估埃及克隆性血小板增多症患者的临床、形态学、细胞遗传学特征和MPL基因突变分析的综合情况，并将这些发现与患者的预后联系起来。背景:血小板增多症可由反应性或自主过程引起，作为影响MPL(骨髓增殖性白血病病毒)、Janus激酶2 (JAK 2)、钙网蛋白(CALR)功能获得活性信号通路的遗传改变的结果。方法:对2020年8月至2021年12月在艾因沙姆斯大学附属医院临床病理科就诊的60例埃及克隆性血小板增多症患者进行研究。实时荧光定量PCR检测全血计数、LDH水平及MPL基因突变。结果:JAK2基因突变发生率高于MPL基因突变发生率(分别为40%和8.3%)。MPL突变比JAK2突变具有侵袭性。年龄较大(68.80±2.28 vs 55.5±8.28)，血栓表现较多(100% vs 58.3%)，血小板计数较高(1221.4±309.70 vs 879.38±215.98)，对治疗反应较差(0%的MPL阳性患者对治疗完全缓解，而JAK2阳性患者为41.7%)，但在巨核细胞形态方面没有明显的形态学差异。结论:MPL W515L/K突变存在于10.3%的原发性血小板增多症埃及患者中，包括我们目前的研究。MPL基因突变对预后有不良影响。此外，JAK2和MPL共存突变的存在是否具有协同不良效应有待进一步的大规模研究评估。
{"title":"Diagnostic Approach and risk stratification of thrombocytosis: Integrating morphological, cytogenetic features and MPL gene mutation","authors":"Olfat M. Hendy, Dina A. Fouad, Shaimaa A. Pessar, Sara M. Aboagiza, Hanan M. Bedair","doi":"10.59204/2314-6788.1053","DOIUrl":"https://doi.org/10.59204/2314-6788.1053","url":null,"abstract":"Objectives: To evaluate the integration of clinical, morphological, cytogenetic features and MPL gene mutation analysis in Egyptian patients with clonal thrombocytosis and correlate these finding to patient’s outcome. Background: Thrombocytosis can be caused by either reactive or autonomous processes as a result of genetic alterations that affect signaling pathway through gain-of-function activity of MPL (myeloproliferative leukemia virus), Janus kinase 2 (JAK 2), calreticulin (CALR). Methods: This study was conducted on 60 Egyptian patients having clonal thrombocytosis attending to clinical pathology department, Ain Shams University Hospitals during the period from August 2020 to December 2021. Complete blood count, LDH level and MPL gene mutation by Real-time PCR were measured. Results: JAK2 gene mutation was more prevalent than MPL gene mutation in our study (40% and 8.3% respectively). MPL mutation has an aggressive nature than JAK2 mutation as evident by; older age (68.80± 2.28 vs 55.5 ± 8.28 accordingly), more thrombotic manifestation (100% vs 58.3%), higher platelets count (1221.4 ± 309.70 vs 879.38 ± 215.98) and poor response to therapy (0% of MPL positive patients achieved complete response to therapy vs 41.7% for JAK2 positive patients) but with no marked morphological difference regarding megakaryocytes morphology. Conclusion: MPL W515L/K mutations present in 10.3% in essential thrombocythemia Egyptian patients, included in our current study. MPL gene mutation has a bad prognostic effect. Moreover, presence of coexisting JAK2 & MPL mutation have a synergistic bad effect to be evaluated in further larger studies.","PeriodicalId":18558,"journal":{"name":"Menoufia Medical Journal","volume":"94 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135719115","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mohsen Elkhayat, Ayman El Lehleh, Noran T. Aboelkhair, Mai Abozeid, Hadeer A. Shahin, Naglaa S. Elabd
{"title":"Role of Mac-2-binding protein glycosylation isomer in assessment of liver fibrosis in patients with chronic hepatitis C virus receiving direct-acting antiviral agents","authors":"Mohsen Elkhayat, Ayman El Lehleh, Noran T. Aboelkhair, Mai Abozeid, Hadeer A. Shahin, Naglaa S. Elabd","doi":"10.59204/2314-6788.1095","DOIUrl":"https://doi.org/10.59204/2314-6788.1095","url":null,"abstract":"","PeriodicalId":18558,"journal":{"name":"Menoufia Medical Journal","volume":"263 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136361516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hesham H. Ahmed, Khaled A. Sha’aban, A. Dokhan, Rafik F. Soliman, Mohammed G. Abdellatif
{"title":"Early and midterm outcomes of left anterior descending coronary artery reconstruction in extensive coronary artery atherosclerosis","authors":"Hesham H. Ahmed, Khaled A. Sha’aban, A. Dokhan, Rafik F. Soliman, Mohammed G. Abdellatif","doi":"10.59204/2314-6788.1097","DOIUrl":"https://doi.org/10.59204/2314-6788.1097","url":null,"abstract":"","PeriodicalId":18558,"journal":{"name":"Menoufia Medical Journal","volume":"26 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85368327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. El-Hawy, A. Shehata, Basem Abd El-Fattah El-Gazzar, Ahmed Mostafa Ahmed Esmaiel, Mai El-Sayad Abd El-Hamid El-Saeedy
{"title":"Assessment of Serum Copeptin Levels in Pediatric Patients with Sickle Cell Anemia","authors":"M. El-Hawy, A. Shehata, Basem Abd El-Fattah El-Gazzar, Ahmed Mostafa Ahmed Esmaiel, Mai El-Sayad Abd El-Hamid El-Saeedy","doi":"10.59204/2314-6788.1040","DOIUrl":"https://doi.org/10.59204/2314-6788.1040","url":null,"abstract":"","PeriodicalId":18558,"journal":{"name":"Menoufia Medical Journal","volume":"25 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81790432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Combined Knee Arthroscopy with Medial Open Wedge High Tibial Osteotomy in Management of Medial Knee Joint Disorder of Adults Varus Knee","authors":"E. E. Agroudy","doi":"10.59204/2314-6788.1056","DOIUrl":"https://doi.org/10.59204/2314-6788.1056","url":null,"abstract":"","PeriodicalId":18558,"journal":{"name":"Menoufia Medical Journal","volume":"61 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73699106","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Correlation between serum uric acid level and angiographic severity of coronary artery disease in patients with acute coronary syndrome","authors":"Mohamed Yahia, Mustafa Ahmad, A. Elkersh","doi":"10.59204/2314-6788.1093","DOIUrl":"https://doi.org/10.59204/2314-6788.1093","url":null,"abstract":"","PeriodicalId":18558,"journal":{"name":"Menoufia Medical Journal","volume":"21 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77574465","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Fady Mohamed ElGendy, Wafaa Abdelaziz Eladrosy, Muhammad Said El-Mekkawy
Objective To evaluate prevalence of hypophosphatemia and hyperphosphatemia and their association with outcome among critically ill children. Background Phosphorus is essential element for various biological functions but data about serum phosphorus level abnormalities among critically ill children are limited. Patients and methods Prospective observational study including children admitted into Pediatric Intensive Care Unit (PICU). Serum phosphorus was measured on admission, day4, day8, and day12. Pediatric Sequential Organ Failure Assessment (pSOFA) score was calculated on admission. Hypophosphatemia and hyperphosphatemia risk factors were determined. Results Hypophosphatemia and hyperphosphatemia were generally non-severe. Hypophosphatemic patients had higher frequency of sepsis on admission compared with normophosphatemic patients (36.2% vs. 12.5%, p=0.019). Frequency of steroid use was higher among hypophosphatemic children (27.7% vs. 6.3%, p=0.017). Hypophosphatemia was associated with longer PICU stay (p=0.028) and hospitalacquired infections (p=0.022). pSOFA and frequencies of both sepsis and elevated serum creatinine were significantly higher among hyperphosphatemic compared with normophosphatemic patients. Hyperphosphatemia was associated with higher mechanical ventilation rate (p=0.018). No significant difference in mortality rate was found between hypophosphatemic, hyperphosphatemic, and normophosphatemic patients. However, phosphorus level on day8 was lower among non-survivors [median (interquartile range): 3.2 (2.3–4.7) vs. 3.9 (3.4–5.2), p=0.046]. Conclusion Phosphorus abnormalities, particularly hypophosphatemia, are common among critically ill children. Hyperphosphatemia was more likely to be found among patients with renal dysfunction, sepsis, and higher pSOFA score. Hypophosphatemia was associated with longer PICU stay, hospital-acquired infections, higher mechanical ventilation rate and pSOFA score.
{"title":"Phosphorus: The Forgotten Electrolyte in The Paediatric Intensive Care Unit","authors":"Fady Mohamed ElGendy, Wafaa Abdelaziz Eladrosy, Muhammad Said El-Mekkawy","doi":"10.59204/2314-6788.1082","DOIUrl":"https://doi.org/10.59204/2314-6788.1082","url":null,"abstract":"Objective To evaluate prevalence of hypophosphatemia and hyperphosphatemia and their association with outcome among critically ill children. Background Phosphorus is essential element for various biological functions but data about serum phosphorus level abnormalities among critically ill children are limited. Patients and methods Prospective observational study including children admitted into Pediatric Intensive Care Unit (PICU). Serum phosphorus was measured on admission, day4, day8, and day12. Pediatric Sequential Organ Failure Assessment (pSOFA) score was calculated on admission. Hypophosphatemia and hyperphosphatemia risk factors were determined. Results Hypophosphatemia and hyperphosphatemia were generally non-severe. Hypophosphatemic patients had higher frequency of sepsis on admission compared with normophosphatemic patients (36.2% vs. 12.5%, p=0.019). Frequency of steroid use was higher among hypophosphatemic children (27.7% vs. 6.3%, p=0.017). Hypophosphatemia was associated with longer PICU stay (p=0.028) and hospitalacquired infections (p=0.022). pSOFA and frequencies of both sepsis and elevated serum creatinine were significantly higher among hyperphosphatemic compared with normophosphatemic patients. Hyperphosphatemia was associated with higher mechanical ventilation rate (p=0.018). No significant difference in mortality rate was found between hypophosphatemic, hyperphosphatemic, and normophosphatemic patients. However, phosphorus level on day8 was lower among non-survivors [median (interquartile range): 3.2 (2.3–4.7) vs. 3.9 (3.4–5.2), p=0.046]. Conclusion Phosphorus abnormalities, particularly hypophosphatemia, are common among critically ill children. Hyperphosphatemia was more likely to be found among patients with renal dysfunction, sepsis, and higher pSOFA score. Hypophosphatemia was associated with longer PICU stay, hospital-acquired infections, higher mechanical ventilation rate and pSOFA score.","PeriodicalId":18558,"journal":{"name":"Menoufia Medical Journal","volume":"23 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135033102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Lateral Extra-articular Tenodesis Combined with Intra-articular ACL Reconstruction in Management of Anterolateral Knee Instability","authors":"E. E. Agroudy","doi":"10.59204/2314-6788.1058","DOIUrl":"https://doi.org/10.59204/2314-6788.1058","url":null,"abstract":"","PeriodicalId":18558,"journal":{"name":"Menoufia Medical Journal","volume":"37 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87385643","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Evaluation of Results of Percutaneous Acetabular Columns Fixation","authors":"E. E. Agroudy","doi":"10.59204/2314-6788.1059","DOIUrl":"https://doi.org/10.59204/2314-6788.1059","url":null,"abstract":"","PeriodicalId":18558,"journal":{"name":"Menoufia Medical Journal","volume":"6 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87728438","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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