Microbial pathogenesis最新文献_第4页

Corrigendum to "Bacillus velezensis LG37 protects Macrobrachium rosenbergii from ammonia nitrogen stress" [Microb. Pathog. 209 (2025) 108098]. “韦氏芽孢杆菌LG37保护罗氏沼虾免受氨氮胁迫”的勘误表[微生物]。中华病理学杂志。209(2025)108098。

IF 3.5 3区医学 Q3 IMMUNOLOGY

Microbial pathogenesis

Pub Date : 2025-12-10 DOI: 10.1016/j.micpath.2025.108227

Guangxin Liu, Hejun Jian, Anfeng Liu, Ce Li, Yiqin Deng, Sigang Fan, Hongling Ma, Changhong Cheng, Jianjun Jiang, Juan Feng, Zhendong Qin, Zhixun Guo

引用次数: 0

Toward plant-based therapeutics: Unravelling the antibacterial, antibiofilm, and antiquorum-sensing actions of Acacia saligna extract 以植物为基础的治疗：揭示金合树提取物的抗菌、抗生物膜和抗群体感应作用

IF 3.5 3区医学 Q3 IMMUNOLOGY

Microbial pathogenesis

Pub Date : 2025-12-09 DOI: 10.1016/j.micpath.2025.108238

Marwa Balaha , Walaa A. Negm , Engy Elekhnawy , Rana M. Aldossari , Alhussain H. Aodah , Aftab Alam , Nehad Ahmed , Mohamed F. Balaha

The universal spread of bacterial infections poses a significant issue in clinical settings. Pseudomonas aeruginosa is among the most commonly detected bacteria in various infections. Besides its resistance to multiple antibiotics, it also exhibits numerous virulence factors that hinder antibiotic treatment. Therefore, we intended to inspect the antibacterial and antivirulence consequences of Acacia saligna plant extract, along with phytochemical analysis of its active components by HPLC. The HPLC analysis identified 15 phenolic acids and flavonoid compounds. The A. saligna ethanol extract (ASE) demonstrated antibacterial activity, with minimum inhibitory concentrations (MICs) ranging from 128 to 1024 μg/mL. Additionally, ASE showed antibiofilm activity, reducing the percentage of vigorous and moderate biofilm-forming isolates from 78.57 % to 21.43 % as measured by the crystal violet assay. A significant downregulation of the QS genes in six out of eight P. aeruginosa isolates, with relative gene expression reduced to 0.1–0.4 for lasI, lasR, and rhlI genes, and 0.3–0.4 for rhlR gene compared to untreated controls. Furthermore, the in vivo antibacterial effects of ASE were demonstrated in P. aeruginosa-infected mice. There was a significant (p < 0.05) reduction in bacterial load in the kidneys, liver, and spleen of the ASE-treated group. Histopathological analysis revealed notable improvements in the kidney, liver, and spleen tissues in the ASE-treated group compared with the positive control. Immunohistochemical examination showed a lessening in the number of inflammatory marker-positive cells (TNF-α and IL-6). Additionally, there was a significant reduction (p < 0.05) in nitric oxide and malondialdehyde in the ASE-treated group. Therefore, ASE could be a promising antibacterial and antivirulence agent for treating P. aeruginosa infections.

细菌感染的普遍传播在临床环境中提出了一个重大问题。铜绿假单胞菌是各种感染中最常检测到的细菌之一。除了对多种抗生素具有耐药性外，它还表现出许多阻碍抗生素治疗的毒力因素。因此，我们打算考察金合欢植物提取物的抗菌和抗毒作用，并利用高效液相色谱分析其有效成分的植物化学成分。HPLC分析鉴定出15种酚酸和类黄酮化合物。saligna乙醇提取物（ASE）具有良好的抑菌活性，最低抑菌浓度（mic）在128 ~ 1024 μg/mL之间。此外，ASE还显示出抗生物膜活性，通过结晶紫法测定，ASE可将强烈和中度形成生物膜的分离株的百分比从78.57%降低到21.43%。8株铜绿假单胞菌中有6株的QS基因显著下调，与未处理的对照组相比，lasI、lasR和rhlI基因的相对基因表达量降至0.1-0.4，rhlR基因的相对基因表达量降至0.3-0.4。此外，ASE在铜绿假单胞菌感染小鼠体内的抑菌作用也得到了证实。ase治疗组肾脏、肝脏和脾脏细菌负荷显著降低（p < 0.05）。组织病理学分析显示，与阳性对照组相比，ase治疗组的肾、肝、脾组织均有显著改善。免疫组化检查显示炎症标志物阳性细胞（TNF-α和IL-6）数量减少。此外，ase治疗组一氧化氮和丙二醛显著降低（p < 0.05）。因此，ASE有望成为一种治疗铜绿假单胞菌感染的有前景的抗菌和抗毒药物。

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引用次数: 0

Phylogenetic, pathotyping and haplotype analysis of Theileria orientalis in crossbred cattle of northern Kerala, India. 印度喀拉拉邦北部杂交牛东方铁杆菌的系统发育、病理分型和单倍型分析。

IF 3.5 3区医学 Q3 IMMUNOLOGY

Microbial pathogenesis

Pub Date : 2025-12-09 DOI: 10.1016/j.micpath.2025.108235

Geethu Reghu, Anju Varghese, Deepa Chundayil Kalarickal, Ajith Kumar Karapparambu Gopalan, Shiji Thozhukkad Moosaripparambil, Anaswara Aravindakshan, Sreenidhi Nellikka Chalapron, Renjith Sebastian, Biju Peer Habeeb, Arun Chungapally, Reghu Ravindran

Theileria orientalis, previously considered non-pathogenic, has recently been associated with clinical outbreaks of theileriosis in India, posing a significant threat to the dairy industry. This study aimed to determine the prevalence and haplotype diversity of T. orientalis in crossbred cattle in northern Kerala and to unravel the potential associations between haplotypes and clinical disease. Blood samples were collected from 149 crossbred cattle in areas with history of Theileria outbreaks and high tick infestation in northern Kerala and screened by microscopy and molecular methods. Piroplasms were detected in 78 (52.3%) of Giemsa-stained blood smears. Further, conventional PCR targeting ∼776 base pair of the major piroplasm surface protein (MPSP) gene successfully amplified the DNA of T. orientalis in 124 (83.4%) of the samples. Infected adults exhibited macrocytic normochromic anaemia, whereas infected calves had normocytic hypochromic anaemia. Red blood cell count, haematocrit, and haemoglobin levels did not differ significantly between infected and non-infected cattle. However, eosinophil and reticulocyte counts were significantly higher in infected animals (p < 0.001). The positive animals were categorized into four groups (Group I-IV) based on haematological findings, microscopy, and PCR results. Phylogenetic and network analyses of the MPSP gene revealed the presence of genotypes 3, 5, and 7 in the present study, with genotype 5 reported for the first time from Kerala and being the most prevalent (47.05%). A total of 43 distinct haplotypes were identified, with genotype 5 being the most diverse. A distinct age-related distribution of T. orientalis genotypes was observed, with type 7 occurring predominantly in calves and type 5 in adults. However, the genotype 7 generally regarded as a pathogenic was also detected in asymptomatic calves as well as in anaemic animals. Insights into genetic diversity, age- related susceptibility, and host responses are vital for developing effective surveillance, targeted control strategies, and sustainable management of the oriental theileriosis.

以前被认为无致病性的东方大肠杆菌最近与印度临床暴发的东方大肠杆菌病有关，对乳制品工业构成重大威胁。本研究旨在确定喀拉拉邦北部杂交牛东方绦虫的患病率和单倍型多样性，并揭示单倍型与临床疾病之间的潜在关联。在喀拉拉邦北部有伊勒菌暴发史和蜱虫高发地区采集了149头杂交牛的血液样本，并采用显微镜和分子方法进行筛选。吉氏菌染色血涂片检出弓形虫78例（52.3%）。此外，针对主要螺质表面蛋白（MPSP）基因的~ 776碱基对的常规PCR成功扩增了124份（83.4%）样品的东方田鼠DNA。受感染的成人表现为大细胞性正色贫血，而受感染的小牛则表现为正色性低色贫血。红细胞计数、红细胞压积和血红蛋白水平在感染牛和未感染牛之间没有显著差异。然而，感染动物的嗜酸性粒细胞和网织红细胞计数明显较高（p < 0.001）。根据血液学、镜检和PCR结果将阳性动物分为四组（I-IV组）。MPSP基因的系统发育和网络分析显示，本研究中存在基因3、5和7型，其中基因5型在喀拉拉邦首次报道，最常见（47.05%）。共鉴定出43种不同的单倍型，其中基因5型的多样性最大。观察到明显的年龄相关性分布，其中7型主要发生在小牛中，5型主要发生在成人中。然而，通常被认为是致病的基因7型也在无症状的小牛和贫血动物中被检测到。深入了解遗传多样性、年龄相关易感性和宿主反应对于制定有效的监测、有针对性的控制策略和可持续的东方线虫病管理至关重要。

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引用次数: 0

Host-directed insights into mycobacterial infections: Toward targeted immunomodulation 宿主导向的分枝杆菌感染研究：定向免疫调节。

IF 3.5 3区医学 Q3 IMMUNOLOGY

Microbial pathogenesis

Pub Date : 2025-12-08 DOI: 10.1016/j.micpath.2025.108233

Arjun M. Menon , Abhinand Kuniyil , Shwetha Susan Thomas , S. Salini , Lekshmi K. Edison , P.C. Parvathi Mohanan , K.B. Arun , Pradeesh Babu , Geetha B. Kumar , Bipin G. Nair , Aravind Madhavan

Mycobacterium tuberculosis, the etiological agent of tuberculosis, is a significant worldwide health threat, especially in resource-limited environments. This review emphasizes that manipulating host metabolic and epigenetic pathways can bolster immune defences and potentially shorten the duration of tuberculosis treatment. The growing prevalence of multidrug-resistant TB and the protracted nature of standard treatments underscore the urgent need for alternative therapeutic approaches. Host-directed treatment has arisen as a promising approach that aims to enhance or redirect the host's innate and adaptive responses rather than targeting the pathogen directly. This strategy focuses on counteracting M. tuberculosis-induced subversion of immune and cellular processes. Approaches under investigation include modulation of host metabolic pathways, stimulation of autophagy, epigenetic reprogramming, and strengthening of immune defense mechanisms to control or eliminate infection. These interventions hold potential for not only overcoming traditional drug resistance but also for accelerating recovery and reducing immunopathology. In this review, we explore recent advances in host-directed therapy research, with particular emphasis on mechanisms involving immunometabolic regulation, epigenetic remodeling, and enhancement of intercellular antimicrobial responses. The advancement of these methodologies may facilitate the creation of more accurate and efficacious tuberculosis treatments. We highlight recent work on three linked areas-immunometabolic regulation, epigenetic control, and autophagy driven antimicrobial activity drawing out the main advances and remaining debates that guide future host-directed approaches. Viewing host-directed therapy through this integrated lens outline a path toward more targeted, durable, and resistance-resilient interventions for tuberculosis.

结核分枝杆菌是结核病的病原，是一个重大的全球健康威胁，特别是在资源有限的环境中。这篇综述强调，操纵宿主代谢和表观遗传途径可以增强免疫防御，并可能缩短结核病治疗的持续时间。耐多药结核病的日益流行和标准治疗的长期性突出表明迫切需要替代治疗方法。宿主定向治疗是一种很有前途的方法，旨在增强或重定向宿主的先天和适应性反应，而不是直接针对病原体。这一战略的重点是对抗结核分枝杆菌引起的免疫和细胞过程的破坏。正在研究的方法包括调节宿主代谢途径、刺激自噬、表观遗传重编程和加强免疫防御机制来控制或消除感染。这些干预措施不仅具有克服传统耐药性的潜力，而且具有加速恢复和减少免疫病理的潜力。在这篇综述中，我们探讨了宿主定向治疗研究的最新进展，特别强调了涉及免疫代谢调节、表观遗传重塑和增强细胞间抗菌反应的机制。这些方法的进步可能有助于创造更准确和有效的结核病治疗方法。我们强调了最近在三个相关领域的工作-免疫代谢调节，表观遗传控制和自噬驱动的抗菌活性，并提出了指导未来宿主导向方法的主要进展和剩余争论。通过这一综合视角观察宿主定向治疗，勾勒出一条更有针对性、更持久、更耐药的结核病干预措施之路。

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引用次数: 0

Nanopore sequencing revealed unique nasopharyngeal microbial communities associated with different SARS-CoV-2 variants 纳米孔测序揭示了与不同SARS-CoV-2变异相关的独特鼻咽部微生物群落。

IF 3.5 3区医学 Q3 IMMUNOLOGY

Microbial pathogenesis

Pub Date : 2025-12-08 DOI: 10.1016/j.micpath.2025.108234

Isnaini Zakiyyah Asyifa , Zahra Noviana , Alvira Rifdah Sativa , Muhammad Mumtaz Adzdzakiy , Ahmad Ridha Al Fiqri , Azzania Fibriani , Anik Budhi Dharmayanthi , Syam Budi Iryanto , Herjuno Ari Nugroho , Andi Yasmon , Sugiyono Saputra , Anggia Prasetyoputri

New variants arising from mutations within the SARS-CoV-2 genome could subsequently lead to increased mortality and morbidity in COVID-19 patients. Microbiota within the human respiratory tract is part of the immune system that can provide protection from infection, including from SARS-CoV-2. Unfortunately, the pathogenesis of various SARS-CoV-2 variants can interfere with the homeostasis of microbial community in the respiratory tract. It is therefore imperative to study the bacterial community profile within the respiratory tract of COVID-19 patients having different SARS-CoV-2 variants. In this study, we identified SARS-CoV-2 variants using whole genome sequencing of naso/oropharyngeal swabs of confirmed COVID-19 patients with Oxford Nanopore Technologies (ONT), followed by 16S rRNA gene amplicon sequencing to assess the bacterial community diversity. Sequencing of 23 selected isolates yielded six SARS-CoV-2 variants, with the majority being B.1.617.2 (Delta), B.1.1.7 (Alpha), and B.1.466.2 (Indonesian). Bacterial community of samples identified as having the Alpha and Delta variants was dominated by Streptococcus, Prevotella. Corynebacterium, and Veillonella, whereas within the Indonesian variant samples Staphylococcus, Salmonella, Enterococcus, and Corynebacterium were predominant. Overall, the bacterial community found in the naso/oropharyngeal swabs of COVID-19 patients has a significant degree of diversity between different SARS-CoV-2 variants, with Prevotella being the overall predominant genus detected (16.2 %).

由SARS-CoV-2基因组突变引起的新变异可能随后导致COVID-19患者的死亡率和发病率增加。人类呼吸道内的微生物群是免疫系统的一部分，可以防止感染，包括SARS-CoV-2。不幸的是，各种SARS-CoV-2变异的发病机制会干扰呼吸道微生物群落的稳态。因此，有必要研究具有不同SARS-CoV-2变异的COVID-19患者呼吸道内的细菌群落特征。本研究采用Oxford Nanopore Technologies （ONT）对确诊COVID-19患者的鼻/口咽拭子进行全基因组测序，鉴定出SARS-CoV-2变异，随后进行16S rRNA基因扩增子测序，评估细菌群落多样性。对选定的23株分离株进行测序，得到6种SARS-CoV-2变体，其中大多数为B.1.617.2 （Delta）、B.1.1.7 （Alpha）和B.1.466.2（印度尼西亚）。经鉴定具有α和δ变异的样品细菌群落以链球菌、普雷沃氏菌为主。而在印度尼西亚变异样本中，葡萄球菌、沙门氏菌、肠球菌和棒状杆菌占主导地位。总体而言，在COVID-19患者鼻/口咽拭子中发现的细菌群落在不同的SARS-CoV-2变体之间具有显著程度的多样性，其中普雷沃氏菌是检测到的总体优势属（16.2%）。

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引用次数: 0

Isolation and application of novel biocontrol agents in the continuous cropping area of garlic (Allium sativum L.) 大蒜连作区新型生物防治剂的分离与应用

IF 3.5 3区医学 Q3 IMMUNOLOGY

Microbial pathogenesis

Pub Date : 2025-12-08 DOI: 10.1016/j.micpath.2025.108231

Chunxue Wang , Jizhen Kan , Kang Chen , Yayu Liu , Jiahui Gong , Xiang Li , Ningyang Li , Chuandong Jiang , Bingyin Peng , Kai Liu , Binghai Du , Yan Zhang , Qiang Zhang , Chengqiang Wang

The traditional cultivation practices of garlic have led to the frequent occurrence of soil-borne diseases and other cropping obstacles. In recent years, shifts in the spectrum of garlic diseases have reduced the effectiveness of conventional control measures, creating an urgent need to identify novel phytopathogens and develop efficient biocontrol strategies. In this study, we systematically screened for novel pathogenic and multifunctional plant-growth-promoting bacteria from garlic seedling tissues and the rhizosphere microbiota. A total of seven Fusarium strains and one novel pathogenic bacterium, Erwinia persicina, were isolated from diseased tissues, thereby expanding the known diversity of garlic phytopathogens. Additionally, sixteen multifunctional plant-growth-promoting strains were identified, among which Paenibacillus polymyxa C2 exhibited broad-spectrum antimicrobial activity, with inhibition rates ranging from 70.17 % to 85.77 %. Pot experiments demonstrated that strain C2 increased garlic seedling height by 109.9 %, and enhanced the activities of soil catalase, neutral phosphatase, and alkaline phosphatase. Meanwhile, P. polymyxa C2 can optimize the rhizosphere microbiota of garlic. Furthermore, through self-selection by garlic seedlings, two synthetic microbial communities (SynComs) were constructed, comprising P. hunanensis Y9, Stenotrophomonas geniculata V4, Pseudomonas moraviensis C3, and P. polymyxa C2. These SynComs significantly promoted garlic growth and reduced the incidence of garlic root rot. This study proposes a novel biocontrol approach using garlic-selected SynComs to suppress phytopathogens while optimizing the rhizosphere microbiota, offering a promising foundation for developing effective microbial inoculants aimed at sustainable agricultural production.

大蒜的传统种植方式导致土传病害的频繁发生和其他种植障碍。近年来，大蒜病害谱的变化降低了传统防治措施的有效性，迫切需要识别新的植物病原体并制定有效的生物防治策略。在这项研究中，我们系统地从大蒜幼苗组织和根际微生物群中筛选了新的致病和多功能植物生长促进细菌。从大蒜病组织中共分离到7株镰刀菌和1株新的病原菌persicina Erwinia，从而扩大了已知大蒜植物病原体的多样性。此外，还鉴定出16株多功能植物促生长菌株，其中多粘类芽孢杆菌C2具有广谱抗菌活性，抑制率在70.17% ~ 85.77%之间。盆栽试验结果表明，C2菌株可使大蒜苗高提高109.9%，提高土壤过氧化氢酶、中性磷酸酶和碱性磷酸酶的活性。同时，多粘草C2对大蒜根际微生物群具有优化作用。通过大蒜幼苗的自我选择，构建了湖南假单胞菌Y9、膝窄养单胞菌V4、莫拉维假单胞菌C3和多粘假单胞菌C2两个合成微生物群落（SynComs）。本研究提出了一种新的生物防治方法，利用大蒜选择的SynComs来抑制植物病原体，同时优化根际微生物群，为开发有效的微生物接种剂以实现农业可持续生产提供了良好的基础。

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引用次数: 0

Salmonella Typhimurium StiP-mediated upregulation of membrane protein Alx drives complement evasion via CFI-dependent C3b degradation 鼠伤寒沙门菌stip介导的膜蛋白Alx上调通过cfi依赖性C3b降解驱动补体逃避。

IF 3.5 3区医学 Q3 IMMUNOLOGY

Microbial pathogenesis

Pub Date : 2025-12-08 DOI: 10.1016/j.micpath.2025.108232

Jin Guan , ZhouPing Zhou , Peng Pan , Kaifeng Chen , Nanwei Wang , Weilin Zhuang , Hailin Fan , Yixin Tang , Jiaqi Wan , Bosen Wang , Chenggang Xu , Ming Liao , Jianmin Zhang

Invasive Salmonella Typhimurium (S. Typhimurium) causes lethal bloodstream infections (BSI), yet its molecular mechanisms remain unclear. We compared serum resistance and cellular invasive capabilities between invasive and non-invasive S. Typhimurium. Invasive strains exhibited significantly enhanced serum resistance (>17-fold survival in 75 % isolates) and HeLa cell invasive ability (minimum bacterial loads 3.93 × 10⁵ CFU/mL) compared to non-invasive strains (<9.8-fold survival; maximum bacterial loads 1.47 × 10⁵ CFU/mL). We next performed pan-genomic analysis and virulence gene database comparisons to elucidate the genetic mechanisms underlying robust serum resistance in invasive S. Typhimurium. We identified 15 differential genes unique to invasive S. Typhimurium, among which the StiP deletion strain (263ΔStiP) showed the greatest serum resistance reduction (2.49-fold). We further explored the role of StiP in host blood environment adaptation and found that 263ΔStiP displayed 3.12-fold reduced HeLa cell adhesion, 3.74-fold lower HeLa cell invasion, 1.92-fold decreased intra-macrophage survival, and 50 % reduced serum resistance versus wild-type 263 (WT263), collectively indicating that StiP is critical for host blood environment adaptation in invasive S. Typhimurium. Mechanistically, StiP upregulates the membrane protein Alx, which recruits complement factor I (CFI) to accelerate C3b degradation, thereby inhibiting classical complement pathway activation and enhancing invasive S. Typhimurium complement evasion. In vivo, 263ΔStiP-infected mice exhibited 5-, 7-, and 4-fold lower bacterial loads in blood, liver, and spleen (P < 0.001), respectively, with reduced pathological damage versus WT263. Thus, this study elucidates the StiP-Alx axis mediating complement evasion in invasive S. Typhimurium.

侵袭性鼠伤寒沙门氏菌（S. Typhimurium）引起致死性血流感染（BSI），但其分子机制尚不清楚。我们比较了侵袭性和非侵袭性鼠伤寒沙门氏菌的血清耐药性和细胞侵袭能力。与非侵入性菌株相比，侵入性菌株的血清耐药性（75%的菌株存活17倍）和HeLa细胞侵袭能力（最小细菌负荷3.93 × 105 CFU/mL）显著增强（最小细菌负荷< 9.8倍，最大细菌负荷1.47 × 105 CFU/mL）。接下来，我们进行了泛基因组分析和毒力基因数据库的比较，以阐明侵袭性鼠伤寒沙门氏菌强大的血清抗性的遗传机制。我们鉴定出15个侵袭性鼠伤寒沙门氏菌特有的差异基因，其中StiP缺失菌株（263ΔStiP）的血清抗性降低幅度最大（2.49倍）。我们进一步探索了StiP在宿主血液环境适应中的作用，发现263ΔStiP与野生型263 （WT263）相比，HeLa细胞粘附降低3.12倍，HeLa细胞侵袭降低3.74倍，巨噬细胞存活降低1.92倍，血清抗性降低50%，共同表明StiP对侵袭性鼠伤寒沙门氏菌的宿主血液环境适应至关重要。在机制上，StiP上调膜蛋白Alx，该蛋白募集补体因子I （CFI）加速C3b降解，从而抑制经典补体途径的激活，增强侵袭性鼠伤寒沙门氏菌的补体逃避。在体内，263ΔStiP-infected小鼠血液、肝脏和脾脏的细菌负荷分别降低了5倍、7倍和4倍（P < 0.001），与WT263相比，病理损伤减少。因此，本研究阐明了侵袭性鼠伤寒沙门氏菌中StiP-Alx轴介导补体逃避的机制。

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引用次数: 0

Microbiome analysis reveals the adverse effects of diarrhea on the gut microbiota of yaks 微生物组分析揭示了腹泻对牦牛肠道菌群的不良影响。

IF 3.5 3区医学 Q3 IMMUNOLOGY

Microbial pathogenesis

Pub Date : 2025-12-06 DOI: 10.1016/j.micpath.2025.108218

Dongjing Wang, Jiangyong Zeng

Diarrhea exhibits a extremely high prevalence among yak calves, significantly affecting the development of the yak industry and resulting in substantial economic losses. Gut microbiota has been demonstrated to be closely related to various diseases, particularly gastrointestinal disorders. However, to date, no studies have specifically explored the relationship between diarrhea in yak calves and gut microbiota. Therefore, this study aims to characterize the alterations of gut microbiota in yak calves during diarrhea. Results indicated that the diversity and abundance of the gut bacterial community in diarrheic yak calves were significantly reduced. In contrast, the gut fungal community remains relatively stable during diarrhea, with no significant changes observed in its diversity and abundance. Although diarrhea does not affect the composition of the dominant phyla of intestinal bacteria and fungi, the abundance of some microbial taxa has experienced significant changes. Specifically, diarrhea resulted in a marked increase in 2 phyla and 14 genera, while a significant decrease was observed in 5 phyla and 137 genera in gut bacterial community. Additionally, 30 genera in the gut fungal community exhibited significant increases, whereas 12 genera demonstrated significant decreases during diarrhea. Notably, we also identified significant alterations in intestinal function during diarrhea, including cytoskeleton and carbohydrate transport, etc. Taken together, this research indicated that diarrhea significantly affects the gut microbiota in yak calves, with more pronounced changes observed in the gut bacterial community. Furthermore, this research also represents a crucial exploration of the composition and alterations of gut microbiota in diarrheic yak calves, which will help prevent and control diarrhea in yaks inhabiting high-altitude regions and promote the development of the yak industry.

牦牛犊牛腹泻发病率极高，严重影响牦牛产业发展，造成重大经济损失。肠道菌群已被证明与多种疾病，特别是胃肠道疾病密切相关。然而，到目前为止，还没有研究专门探讨牦牛犊牛腹泻与肠道微生物群之间的关系。因此，本研究旨在描述牦牛犊牛腹泻期间肠道菌群的变化。结果表明，腹泻牦牛犊牛肠道细菌群落的多样性和丰度显著降低。相比之下，肠道真菌群落在腹泻期间保持相对稳定，其多样性和丰度没有明显变化。虽然腹泻不影响肠道细菌和真菌优势门的组成，但一些微生物类群的丰度发生了显著变化。其中腹泻导致肠道菌群2门14属明显增加，5门137属明显减少。此外，肠道真菌群落中的30个属在腹泻期间显着增加，而12个属显着减少。值得注意的是，我们还发现腹泻期间肠道功能的显著改变，包括细胞骨架和碳水化合物运输等。综上所述，本研究表明，腹泻显著影响牦牛犊牛的肠道微生物群，肠道细菌群落的变化更为明显。此外，本研究对腹泻牦牛犊牛肠道菌群组成及变化的研究具有重要意义，有助于防治高海拔地区牦牛腹泻，促进牦牛产业的发展。

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引用次数: 0

Corrigendum to "Assessment of inflammatory biomarkers and their correlation with wound healing status following application of chitosan hydrogel with PRP" [Microb. Pathog. 210 (2026) 108146]. “评估炎症生物标志物及其与PRP应用壳聚糖水凝胶后伤口愈合状态的相关性”的勘误表[微生物]。中华病毒学杂志，2010(5):387 - 387。

IF 3.5 3区医学 Q3 IMMUNOLOGY

Microbial pathogenesis

Pub Date : 2025-12-06 DOI: 10.1016/j.micpath.2025.108219

Junwei Du, Hailang Shi, Zahid Manzoor, Adnan Hassan Tahir, Amber Fatima, Tehreem Fayyaz, Muhammad Abu Bakr Shabbir, Muhammad Kashif Maan

引用次数: 0

Meta-analysis of faecal microbiome studies followed by machine learning to identify intestinal disease-specific taxonomic signatures 对粪便微生物组研究进行荟萃分析，随后进行机器学习，以识别肠道疾病特异性分类特征

IF 3.5 3区医学 Q3 IMMUNOLOGY

Microbial pathogenesis

Pub Date : 2025-12-05 DOI: 10.1016/j.micpath.2025.108221

Debasree Sarkar , Paramita Roy , Sudipto Saha

Alterations in the gut microbiome have been found to be associated with both inflammatory bowel disease (IBD) and irritable bowel syndrome (IBS), suggesting that interaction with the microbiota might drive pathogenesis. Identifying microbial profiles for IBS and IBD will help in the development of treatment strategies tailored to each illness, particularly when coexisting symptoms make clinical management difficult. In this study a meta-analysis approach followed by machine learning was used for elucidating the differences in microbial composition and community dynamics in IBS and IBD respectively. Machine learning models developed using Random Forest classifier was able to achieve an accuracy of 98 % on training data and 100 % on blind dataset, and the distinctive role of Pseudomonas-associated microbial network, which also includes Streptococcus, Stenotrophomonas, Actinomyces and Intestinibacter, was clearly observed in IBS.

肠道微生物群的改变已被发现与炎症性肠病（IBD）和肠易激综合征（IBS）相关，这表明与微生物群的相互作用可能驱动发病机制。确定肠易激综合征和肠易激综合征的微生物特征将有助于制定针对每种疾病的治疗策略，特别是在共存症状使临床管理变得困难的情况下。在这项研究中，采用荟萃分析方法和机器学习方法分别阐明了IBS和IBD中微生物组成和群落动态的差异。使用Random Forest分类器开发的机器学习模型在训练数据上的准确率达到98%，在盲数据上的准确率达到100%，并且在IBS中可以清楚地观察到假单胞菌相关微生物网络的独特作用，该网络还包括链球菌、窄养单胞菌、放线菌和无肠杆菌。

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