Pub Date : 1900-01-01DOI: 10.5984/JJPNSOCLASERDENT.29.10
S. Watanabe, Akira Kouno, Tomoyuki Hongo, Kanako Yao, T. Okiji
{"title":"A Review of LLLT in Dentistry:: Current Evidence from Systematic Reviews and Randomized Clinical Trials","authors":"S. Watanabe, Akira Kouno, Tomoyuki Hongo, Kanako Yao, T. Okiji","doi":"10.5984/JJPNSOCLASERDENT.29.10","DOIUrl":"https://doi.org/10.5984/JJPNSOCLASERDENT.29.10","url":null,"abstract":"","PeriodicalId":187459,"journal":{"name":"Journal of Japanese Society for Laser Dentistry","volume":"21 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125912763","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.5984/JJPNSOCLASERDENT.26.17
Naohiro Iwata, K. Yokota, Youhei Hirota, K. Yasuo, K. Yoshikawa, Kazuyo Yamamoto
: Various studies have revealed that the Er:YAG laser is effective in endodontic, periodontal and surgical treat-ments, and it is used in actual clinical practice. In particular, the Er:YAG laser shows excellent clinical effects when cutting hard tooth tissue, and there have been many studies on adhesive restoration involving teeth irradiated with Er:YAG laser. In this study, focusing on the one-bottle one-step bonding system, we performed tensile bond and marginal leakage tests to evalu-ate its adhesive properties to enamel and dentin irradiated with Er:YAG laser.
{"title":"Tensile Bond Strength and Adaptability of One-bottle One-step Bonding Systems to Dental Hard Tissues Irradiated by Er:YAG Laser","authors":"Naohiro Iwata, K. Yokota, Youhei Hirota, K. Yasuo, K. Yoshikawa, Kazuyo Yamamoto","doi":"10.5984/JJPNSOCLASERDENT.26.17","DOIUrl":"https://doi.org/10.5984/JJPNSOCLASERDENT.26.17","url":null,"abstract":": Various studies have revealed that the Er:YAG laser is effective in endodontic, periodontal and surgical treat-ments, and it is used in actual clinical practice. In particular, the Er:YAG laser shows excellent clinical effects when cutting hard tooth tissue, and there have been many studies on adhesive restoration involving teeth irradiated with Er:YAG laser. In this study, focusing on the one-bottle one-step bonding system, we performed tensile bond and marginal leakage tests to evalu-ate its adhesive properties to enamel and dentin irradiated with Er:YAG laser.","PeriodicalId":187459,"journal":{"name":"Journal of Japanese Society for Laser Dentistry","volume":"21 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126363499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.5984/JJPNSOCLASERDENT.3.65
H. Wakabayashi, M. Hamba, Koukichi Matsumoto, T. Nakayama
Effect of GaALAs semiconductor laser irradiation on activities of the neurons in the trigeminal subuncleus caudalis was electrophysiologically investigated in rats. Extrace llular potentials of the caudal neurons were induced by electric stimulation of the ipsilateral lower incisor pulp and laser irradiation was carried out on the cervical surface of the incisor. Changes in firing discharges and numbers of evoked spikes of the caudal neurons were compared before and after irradiation of the laser.1. Laser irradiation suppressed significantly the late discharges evoked by excitatory inputs from C-fiber afferents, but did not the early discharges evoked by inputs from A δ-afferent fibers.2. Laser irradiation could not suppress wind-up phenomenon of the caudal neurons which was induced by repetitive stimulation of the tooth pulp. However, previous irradiation of the laser before tooth pulp stimulation was effective not to induce wind-up of neurons.3. Non-laser light of a tungsten lamp did not suppress the spike discharges of caudal neurons.4. Reduction in the spike numbers was not significantly observed by the irradiation applied on the “GOUKOKU” point.5. Further investigations about the effect of low power laser irradiation on free nerve endings of C-fiber should be required.
{"title":"Electrophysiological Study of Irradiation of Semiconductor Laser on the Activity of the Trigeminal Subnucleus Caudal Neurons","authors":"H. Wakabayashi, M. Hamba, Koukichi Matsumoto, T. Nakayama","doi":"10.5984/JJPNSOCLASERDENT.3.65","DOIUrl":"https://doi.org/10.5984/JJPNSOCLASERDENT.3.65","url":null,"abstract":"Effect of GaALAs semiconductor laser irradiation on activities of the neurons in the trigeminal subuncleus caudalis was electrophysiologically investigated in rats. Extrace llular potentials of the caudal neurons were induced by electric stimulation of the ipsilateral lower incisor pulp and laser irradiation was carried out on the cervical surface of the incisor. Changes in firing discharges and numbers of evoked spikes of the caudal neurons were compared before and after irradiation of the laser.1. Laser irradiation suppressed significantly the late discharges evoked by excitatory inputs from C-fiber afferents, but did not the early discharges evoked by inputs from A δ-afferent fibers.2. Laser irradiation could not suppress wind-up phenomenon of the caudal neurons which was induced by repetitive stimulation of the tooth pulp. However, previous irradiation of the laser before tooth pulp stimulation was effective not to induce wind-up of neurons.3. Non-laser light of a tungsten lamp did not suppress the spike discharges of caudal neurons.4. Reduction in the spike numbers was not significantly observed by the irradiation applied on the “GOUKOKU” point.5. Further investigations about the effect of low power laser irradiation on free nerve endings of C-fiber should be required.","PeriodicalId":187459,"journal":{"name":"Journal of Japanese Society for Laser Dentistry","volume":"53 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125076715","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.5984/JJPNSOCLASERDENT.9.13
T. Eguro, M. Ogawa, M. Tanabe, T. Maeda, Hisayoshi Tanaka
Recently, the Er: YAG laser has been applied to some kinds of dental treatments. The aim of this study was to observe the surface changes of the restorative alloys irradiated by the Er: YAG laser. The specimens (8mm ∅×4mm high) were prepared from 5 alloys. [G;Type II gold alloy (CASTING GOLD M.C. TYPE II GC, Tokyo, Japan), 12% gold-silver-palladium alloy (PD; KINPARA S12% ISHIHUKU, Tokyo, Japan), silver alloy (S;MIRO 3, GC, Tokyo, Japan), amalgam (AM;SPHERICAL-D CAPSULE, SHOFU, Tokyo, Japan), gallium alloy (GA;Galliumalloy GF, Tokuriki, Tokyo, Japan)]. The specimens of G, PD and S were polished with silicon carbite paper to #1500. For AM and GA, two type of specimens were prepared [polished to #1500 (poAM, poGA), un-polished (noAM, noGA)]. The Er: YAG laser (KaVo K.E.Y. Laser) was irradiated to following 6 conditions: energy; 300mJ, pulse frequency; 1Hz, focal distance; 12mm, fine water mist; with/without, total pulse; 1, 3, 10 shots. The irradiated surface of specimens were observed using OPTIPHOTO (Nikon, Tokyo, Japan) and EPMA (Shimadzu, Kyoto, Japan). The results were as follows: 1. The Er: YAG laser irradiation did not affect G and PD. 2. S, poAM, noAM, poGA, noGA were melted by the Er: YAG laser irradiation. The surface of S was like beegum.The surface of poAM and noAM was crater form. The surface of poGA and noGA was like a thin film. 3. In cases of 10 shots irradiation to S, poAM, noAM, poGA, noGA and 3 shots irradiation to S and noGA, irradiation with the fine water mist spray caused more effect than irradiation without the fine water mist. In the other cases, the effects of fine water mist spray were little.
{"title":"Surface Observation of the Er: YAG Laser Irradiated Restorative Alloys","authors":"T. Eguro, M. Ogawa, M. Tanabe, T. Maeda, Hisayoshi Tanaka","doi":"10.5984/JJPNSOCLASERDENT.9.13","DOIUrl":"https://doi.org/10.5984/JJPNSOCLASERDENT.9.13","url":null,"abstract":"Recently, the Er: YAG laser has been applied to some kinds of dental treatments. The aim of this study was to observe the surface changes of the restorative alloys irradiated by the Er: YAG laser. The specimens (8mm ∅×4mm high) were prepared from 5 alloys. [G;Type II gold alloy (CASTING GOLD M.C. TYPE II GC, Tokyo, Japan), 12% gold-silver-palladium alloy (PD; KINPARA S12% ISHIHUKU, Tokyo, Japan), silver alloy (S;MIRO 3, GC, Tokyo, Japan), amalgam (AM;SPHERICAL-D CAPSULE, SHOFU, Tokyo, Japan), gallium alloy (GA;Galliumalloy GF, Tokuriki, Tokyo, Japan)]. The specimens of G, PD and S were polished with silicon carbite paper to #1500. For AM and GA, two type of specimens were prepared [polished to #1500 (poAM, poGA), un-polished (noAM, noGA)]. The Er: YAG laser (KaVo K.E.Y. Laser) was irradiated to following 6 conditions: energy; 300mJ, pulse frequency; 1Hz, focal distance; 12mm, fine water mist; with/without, total pulse; 1, 3, 10 shots. The irradiated surface of specimens were observed using OPTIPHOTO (Nikon, Tokyo, Japan) and EPMA (Shimadzu, Kyoto, Japan). The results were as follows: 1. The Er: YAG laser irradiation did not affect G and PD. 2. S, poAM, noAM, poGA, noGA were melted by the Er: YAG laser irradiation. The surface of S was like beegum.The surface of poAM and noAM was crater form. The surface of poGA and noGA was like a thin film. 3. In cases of 10 shots irradiation to S, poAM, noAM, poGA, noGA and 3 shots irradiation to S and noGA, irradiation with the fine water mist spray caused more effect than irradiation without the fine water mist. In the other cases, the effects of fine water mist spray were little.","PeriodicalId":187459,"journal":{"name":"Journal of Japanese Society for Laser Dentistry","volume":"11 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124755266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.5984/JJPNSOCLASERDENT.2.11
K. Ishikawa, M. Goto, Yuichi Nakanishi, Hisaya Ogata, H. Kurokawa, Hirotoshi Nakagawa, E. Kubota, T. Katsuki
Recently the laser doppler velocimeter has been developped as aids in evaluation of microcircuratory flow in a variety of human tissue. We have used this instrument to measure viability of the flaps which were transplanted to the reconstruction of maxillo-facial region.The blood circulation of D-P flaps and Abbe flap were measured by laser doppler verocimeter. The analog data of the blood circulation was analyzed by using personal computer. The software for data analysis was developped by ourselves.The laser doppler verocimeter is a non-invasive instrument capable of instantaneous and continuous evalation of local flow in tissue microcirculation. It is fit to monitor viability of flaps used oral and maxillo-facial surgery.
{"title":"Measurement of Flap Blood Flow by Laser Doppler Velocimeter in Oral Surgery","authors":"K. Ishikawa, M. Goto, Yuichi Nakanishi, Hisaya Ogata, H. Kurokawa, Hirotoshi Nakagawa, E. Kubota, T. Katsuki","doi":"10.5984/JJPNSOCLASERDENT.2.11","DOIUrl":"https://doi.org/10.5984/JJPNSOCLASERDENT.2.11","url":null,"abstract":"Recently the laser doppler velocimeter has been developped as aids in evaluation of microcircuratory flow in a variety of human tissue. We have used this instrument to measure viability of the flaps which were transplanted to the reconstruction of maxillo-facial region.The blood circulation of D-P flaps and Abbe flap were measured by laser doppler verocimeter. The analog data of the blood circulation was analyzed by using personal computer. The software for data analysis was developped by ourselves.The laser doppler verocimeter is a non-invasive instrument capable of instantaneous and continuous evalation of local flow in tissue microcirculation. It is fit to monitor viability of flaps used oral and maxillo-facial surgery.","PeriodicalId":187459,"journal":{"name":"Journal of Japanese Society for Laser Dentistry","volume":"9 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122093641","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.5984/jjpnsoclaserdent.32.9
Yutaro Kitanaka, Y. Takeuchi, K. Hiratsuka, H. Nitta, T. Iwata, A. Aoki
{"title":"Application of Antimicrobial Photodynamic Therapy in Periodontal Treatment","authors":"Yutaro Kitanaka, Y. Takeuchi, K. Hiratsuka, H. Nitta, T. Iwata, A. Aoki","doi":"10.5984/jjpnsoclaserdent.32.9","DOIUrl":"https://doi.org/10.5984/jjpnsoclaserdent.32.9","url":null,"abstract":"","PeriodicalId":187459,"journal":{"name":"Journal of Japanese Society for Laser Dentistry","volume":"51 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131343135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.5984/JJPNSOCLASERDENT.9.38
A. Shinohara, Mugio Kato, T. Imai, Kenji Yoshida, S. Yamada, K. Kurita
It is generally recognized that the lower power Nd: YAG laser provides some therapeutic effects on tissue without irreversible changes. On the other hand, high power laser causes irreversible changes. The criterion about low and high power laser is, however, not established. The aim of this study is to verify the effect of lower and high power laser on wound healing of rat skin by measuring blood flow and histomorphologic findings with HE and SEM. Each laser were irradiated to the center of 6mm round full-thickness skin defect at the back of the rat at either (1) 300mW×3min, (2) 500mW×3min, (3) 700mW×3min, (4) 300mW×20min, (5) 500mw×20min, (6) 700mW×20min.The results obtained are as follows;1) The histological findings showed that irradiation time caused more tissue damage in the deeper layer, whereas irradiation power caused more damage to the surrounding tissue.2) Small craters were starred on the bottom of the damaged tissue by laser irradiation at 700 mW x 20min. The formation of these craters was related to the absorption of laser irradiation.3) On the irradiated area, blood volume decreased significantly as irradiation power increased. However, both blood flow and tissue damage increased as irradiation time increased. As for the surrounding tissue, the findings showed an increase in the blood flow and tissue damage as irra diation power increased.4) High power laser at 700mW caused tissue damage regardless of irradiation time. On the other hand, 500mW irradiation power caused minimum damage to the tissue. Therefore, the range between 300 to 500mW and the irraditation time over 3min to less than 20min are classified as a lower power laser.
{"title":"Fundamental Studies on Tissue Reaction after Low Power Laser Irradiation","authors":"A. Shinohara, Mugio Kato, T. Imai, Kenji Yoshida, S. Yamada, K. Kurita","doi":"10.5984/JJPNSOCLASERDENT.9.38","DOIUrl":"https://doi.org/10.5984/JJPNSOCLASERDENT.9.38","url":null,"abstract":"It is generally recognized that the lower power Nd: YAG laser provides some therapeutic effects on tissue without irreversible changes. On the other hand, high power laser causes irreversible changes. The criterion about low and high power laser is, however, not established. The aim of this study is to verify the effect of lower and high power laser on wound healing of rat skin by measuring blood flow and histomorphologic findings with HE and SEM. Each laser were irradiated to the center of 6mm round full-thickness skin defect at the back of the rat at either (1) 300mW×3min, (2) 500mW×3min, (3) 700mW×3min, (4) 300mW×20min, (5) 500mw×20min, (6) 700mW×20min.The results obtained are as follows;1) The histological findings showed that irradiation time caused more tissue damage in the deeper layer, whereas irradiation power caused more damage to the surrounding tissue.2) Small craters were starred on the bottom of the damaged tissue by laser irradiation at 700 mW x 20min. The formation of these craters was related to the absorption of laser irradiation.3) On the irradiated area, blood volume decreased significantly as irradiation power increased. However, both blood flow and tissue damage increased as irradiation time increased. As for the surrounding tissue, the findings showed an increase in the blood flow and tissue damage as irra diation power increased.4) High power laser at 700mW caused tissue damage regardless of irradiation time. On the other hand, 500mW irradiation power caused minimum damage to the tissue. Therefore, the range between 300 to 500mW and the irraditation time over 3min to less than 20min are classified as a lower power laser.","PeriodicalId":187459,"journal":{"name":"Journal of Japanese Society for Laser Dentistry","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125683049","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.5984/jjpnsoclaserdent.27.63
Y. Daigo, Erina Daigo, H. Fukuoka, M. Ishikawa, K. Kakimoto, Kazuya Takahashi, Y. Komasa
The utility of laser irradiation has been demonstrated in basic and clinical studies, albeit with an associated risk of tissue damage. We previously tested a combination of high reactive level laser therapy (HLLT) and low reactive level laser therapy (LLLT) using a carbon dioxide laser for enhanced post-extraction wound healing and observed irreversible changes in the pulps of the teeth adjacent to the extracted tooth. The aim of this study was to elucidate the histopathological changes in the pulps of teeth adjacent to the site of carbon dioxide laser irradiation performed to achieve enhanced wound healing after tooth extraction ; here we report the significance of care for the adjacent tissues in laser irradiation therapy. A total of 20 male Wistar rats (5 weeks old) were divided into an irradiation group that received HLLT combined with LLLT and a non-irradiation (control) group that did not. A tooth socket model was prepared by extracting the maxillary left first molar. HLLT and LLLT were respectively performed immediately and 1 day after tooth extraction. Laser-stimulated tissues surrounding the extracted tooth, namely, the socket and adjacent teeth, were collected from each rat both 7 and 10 days after tooth extraction. The obtained specimens were fixed in 4% paraformaldehyde, decalcified in 10% ethylenediaminetetraacetic solution, and then embedded in paraffin. Serial sagittal sections were stained with hematoxylin and eosin for histopathological examination. Laserinduced injuries, such as enamel ablation and dentin carbonization, were absent 7 days after tooth extraction in both groups. Formation of reparative dentin was observed along the rim of the laser-irradiated area in the dental pulps in the irradiation group but not the control group. Both groups showed no signs of inflammation in pulp tissues. Enamel and dentin injuries were not observed in either group at 10 days after tooth extraction, similar to the findings at 7 days after tooth extraction. Formation of reparative dentin was much more prominent at 10 days after tooth extraction than at 7 days in the irradiation group. In contrast, no formation of reparative dentin was observed at either time point in the control group. These results show that carbon dioxide laser irradiation induces the formation of reparative dentin in the pulps of teeth adjacent to the 〒 546-0032 大阪府大阪市東住吉区東田辺 2-2-14 TEL 06-7502-2649 FAX 06-7502-2649 2-2-14 Higashitanabe, Higashisumiyoshi-ku, Osaka-city, Osaka 546-0032, Japan. TEL +81-6-7502-2649 FAX +81-6-7502-2649 64 日本レーザー歯学会誌 27:63-70,2016 extracted tooth socket. In sum, carbon dioxide laser irradiation at the low reactive level appears to induce irreversible changes in the tissues surrounding the laser treatment site. (J. Jpn. Soc. Laser Dent. 27:63 ~ 70, 2016 Reprint requests to Dr. DAIGO) Key words= Carbon dioxide laser, Adjacent tooth, Dental pulp, Reparative dentin キーワード=炭酸ガスレーザー,隣接歯,歯髄組織,修復象牙質
{"title":"Effect on Adjacent Teeth of Carbon Dioxide Laser Irradiation for Enhanced Post-extraction Wound Healing in Rat","authors":"Y. Daigo, Erina Daigo, H. Fukuoka, M. Ishikawa, K. Kakimoto, Kazuya Takahashi, Y. Komasa","doi":"10.5984/jjpnsoclaserdent.27.63","DOIUrl":"https://doi.org/10.5984/jjpnsoclaserdent.27.63","url":null,"abstract":"The utility of laser irradiation has been demonstrated in basic and clinical studies, albeit with an associated risk of tissue damage. We previously tested a combination of high reactive level laser therapy (HLLT) and low reactive level laser therapy (LLLT) using a carbon dioxide laser for enhanced post-extraction wound healing and observed irreversible changes in the pulps of the teeth adjacent to the extracted tooth. The aim of this study was to elucidate the histopathological changes in the pulps of teeth adjacent to the site of carbon dioxide laser irradiation performed to achieve enhanced wound healing after tooth extraction ; here we report the significance of care for the adjacent tissues in laser irradiation therapy. A total of 20 male Wistar rats (5 weeks old) were divided into an irradiation group that received HLLT combined with LLLT and a non-irradiation (control) group that did not. A tooth socket model was prepared by extracting the maxillary left first molar. HLLT and LLLT were respectively performed immediately and 1 day after tooth extraction. Laser-stimulated tissues surrounding the extracted tooth, namely, the socket and adjacent teeth, were collected from each rat both 7 and 10 days after tooth extraction. The obtained specimens were fixed in 4% paraformaldehyde, decalcified in 10% ethylenediaminetetraacetic solution, and then embedded in paraffin. Serial sagittal sections were stained with hematoxylin and eosin for histopathological examination. Laserinduced injuries, such as enamel ablation and dentin carbonization, were absent 7 days after tooth extraction in both groups. Formation of reparative dentin was observed along the rim of the laser-irradiated area in the dental pulps in the irradiation group but not the control group. Both groups showed no signs of inflammation in pulp tissues. Enamel and dentin injuries were not observed in either group at 10 days after tooth extraction, similar to the findings at 7 days after tooth extraction. Formation of reparative dentin was much more prominent at 10 days after tooth extraction than at 7 days in the irradiation group. In contrast, no formation of reparative dentin was observed at either time point in the control group. These results show that carbon dioxide laser irradiation induces the formation of reparative dentin in the pulps of teeth adjacent to the 〒 546-0032 大阪府大阪市東住吉区東田辺 2-2-14 TEL 06-7502-2649 FAX 06-7502-2649 2-2-14 Higashitanabe, Higashisumiyoshi-ku, Osaka-city, Osaka 546-0032, Japan. TEL +81-6-7502-2649 FAX +81-6-7502-2649 64 日本レーザー歯学会誌 27:63-70,2016 extracted tooth socket. In sum, carbon dioxide laser irradiation at the low reactive level appears to induce irreversible changes in the tissues surrounding the laser treatment site. (J. Jpn. Soc. Laser Dent. 27:63 ~ 70, 2016 Reprint requests to Dr. DAIGO) Key words= Carbon dioxide laser, Adjacent tooth, Dental pulp, Reparative dentin キーワード=炭酸ガスレーザー,隣接歯,歯髄組織,修復象牙質","PeriodicalId":187459,"journal":{"name":"Journal of Japanese Society for Laser Dentistry","volume":"161 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131818628","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.5984/JJPNSOCLASERDENT.21.197
Y. Yasuda, Takashi Saito
Reparative dentin is formed in the dental pulp in response to various external stimuli such as caries and abrasion. Carbon dioxide laser has been used to accelerate the formation of reparative dentin on the exposed pulp surface. However, the mechanism by which carbon dioxide laser irradiation stimulates mineralization in direct pulp capping treatment is not fully understood. We examined the effects of carbon dioxide laser irradiation on mineralization in rat dental pulp cells. Rat dental pulp cells were irradiated with a carbon dioxide laser at an output power of 2 W for 20, 40, and 60 s and were cultured in media containing ascorbic acid and β-glycerophosphate. Cell viability was examined 24 h after laser irradiation by a modified MTT assay. Alizarin Red S staining was performed 10 days after laser irradiation. The amounts of collagen secreted from the cells after irradiation were quantified following Sirius Red staining. The expression levels of collagen type I and HSP47, collagen-binding stress protein, were analyzed by real-time PCR. HSP47 protein expression was examined by Western blotting. The cell viability was not affected by laser irradiation at 2 W for up to 40 s. However, it was significantly decreased by 20% at 60 s. The amount of mineralization after 10 days of irradiation at 2 W for 40 s was significantly increased in comparison to the other conditions. The extracellular collagen production was significantly increased, by 73% on day 2 and 38% on day 4, after laser irradiation. Although collagen type I gene expression was not changed by laser irradiation, HSP47 gene and protein expression was induced within 12 h and 24 h, respectively. These results suggested that carbon dioxide laser irradiation stimulated mineralization in dental pulp cells by increasing HSP47 expression. (J. Jpn. Soc. Laser Dent. 21:197 ~ 202, 2010 Reprint requests to Dr. YASUDA) Key words= Carbon dioxide laser, Dental pulp cells, Odontoblasts, Mineralization キーワード=炭酸ガスレーザー,歯髄細胞,象牙芽細胞,石灰化 〒 061-0293 北海道石狩郡当別町金沢 1757 TEL 0133-23-1726 FAX 0133-23-1726 1757 Tobetsu, Hokkaido 061-0293, Japan. TEL +81-133-23-1726 FAX +81-133-23-1726 198 日本レーザー歯学会誌 21:197-202,2010 牙質で覆い,かつ歯髄の炎症反応が認められないことが理 想とされる。 現在,レーザーが切開,止血,根管内殺菌や歯石除去な どその適応の広さから歯科臨床において広く利用されてい る。その中でも半導体レーザーなどの低出力レーザーには 創傷治癒の促進効果があり9),抜歯創や非感染性の露髄面 への応用により硬組織形成促進効果が観察されている。ま た,培養細胞レベルにおいても半導体レーザー照射により 細胞分化促進や石灰化結節の増大などが認められてい る10-12)。一方で,炭酸ガスレーザーなどの高出力レーザー は主に組織の蒸散により切開や病巣部の除去などを目的と してきた13)。現在では低出力照射により石灰化誘導やイ ンプラント周囲骨の再生への応用が期待されている14,15)。 HSP(heat shock protein)は,熱や薬剤刺激などのス トレスによって発現が誘導され,変性タンパク質の凝集抑 制を行うタンパク質として知られている16,17)。また,正常 な細胞でも構成的に発現しており,新生タンパク質の フォールディングや細胞内輸送などの重要な機能に関わっ ていることも明らかになってきた18,19)。その中でも HSP47 はコラーゲン特異的分子シャペロンで,コラーゲ ンに特異的に結合し,その合成過程におけるフォールディ ングを正しく導くことでコラーゲン産生に必須な役割を持 つことが知られている20,21)。 これまでに,直接覆髄実験において露髄面への炭酸ガス レーザー照射の有用性が示唆されている22)が,培養歯髄 細胞を用いた細胞レベルでの石灰化への影響についての報 告はほとんどない。そこで今回,炭酸ガスレーザー低出力 照射の象牙芽細胞の石灰化誘導効果について今までに明ら かとしてきたデータを提示23)し考察する。 炭酸ガスレーザー照射の細胞生存率への影響 ラット歯髄細胞に対して 2 W 出力で 20,40 および
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Pub Date : 1900-01-01DOI: 10.5984/JJPNSOCLASERDENT.23.131
Daisuke Naito, K. Kakimoto, Kazuya Takahashi, Y. Komasa
Dentures are comprised of resin and metal materials. These materials are joined using chemicals or mechanically by making under-cuts, but spaces remaining between the materials cause denture fracture and oral bacterial infection. To solve this problem, we performed a basic study to evaluate the applicability of laser-assisted metal and plastic (LAMP) joining for the direct joining of cobalt-chromium alloy for casting (COBALTAN, Shofu Inc., Kyoto, Japan) and acrylic resin for a thermoplastic base (ACRYJET, High-Dental Japan, Osaka, Japan). LAMP joining is a technique that facilitates the laser joining of resin and metal and was developed by the Laser Welding and Materials Processing, Welding Research Institute, Osaka University. LAMP joining achieves a very strong joint between acrylic resin and cobalt-chromium alloy. As a result, most samples of thermoplastic acrylic resin for denture suffer plastic deformation in tensile shear tests, and some samples broke at a position away from the joint interface on thermoplastic acrylic resin for dentures, prior to fracture at the joint interface. Although it was not possible to measure the bonding strength to line profile, in this experiment, the joints possessed very strong tensile shear strengths of not less than 4.9 MPa. Sufficient bonding strength was obtained between the thermoplastic acrylic resin for dentures and the cobalt-chromium alloy for casting by LAMP joining. When the bonded interface was observed under SEM, fine air bubbles characteristic of LAMP joining were observed, and no micro-level space was present in the bonded interface. It is suggested that cobalt-chromium alloy for casting and acrylic resin for a thermoplastic base can be joined with no space in the bond interface by using LAMP joining without special surface processing. (J. Jpn. Soc. Laser Dent. 23:131 ~ 136, 2012 Reprint requests to Dr. NAITO) Key words= Laser joining, Dissimilar materials joining, Thermoplastic acrylic resin, Cobalt-chromium cast alloy キーワード=レーザー接合,異材接合,熱可塑性アクリル樹脂,コバルトクロム合金
{"title":"Basic Study on Laser Joining of Resin for Denture Base and Dental Alloy","authors":"Daisuke Naito, K. Kakimoto, Kazuya Takahashi, Y. Komasa","doi":"10.5984/JJPNSOCLASERDENT.23.131","DOIUrl":"https://doi.org/10.5984/JJPNSOCLASERDENT.23.131","url":null,"abstract":"Dentures are comprised of resin and metal materials. These materials are joined using chemicals or mechanically by making under-cuts, but spaces remaining between the materials cause denture fracture and oral bacterial infection. To solve this problem, we performed a basic study to evaluate the applicability of laser-assisted metal and plastic (LAMP) joining for the direct joining of cobalt-chromium alloy for casting (COBALTAN, Shofu Inc., Kyoto, Japan) and acrylic resin for a thermoplastic base (ACRYJET, High-Dental Japan, Osaka, Japan). LAMP joining is a technique that facilitates the laser joining of resin and metal and was developed by the Laser Welding and Materials Processing, Welding Research Institute, Osaka University. LAMP joining achieves a very strong joint between acrylic resin and cobalt-chromium alloy. As a result, most samples of thermoplastic acrylic resin for denture suffer plastic deformation in tensile shear tests, and some samples broke at a position away from the joint interface on thermoplastic acrylic resin for dentures, prior to fracture at the joint interface. Although it was not possible to measure the bonding strength to line profile, in this experiment, the joints possessed very strong tensile shear strengths of not less than 4.9 MPa. Sufficient bonding strength was obtained between the thermoplastic acrylic resin for dentures and the cobalt-chromium alloy for casting by LAMP joining. When the bonded interface was observed under SEM, fine air bubbles characteristic of LAMP joining were observed, and no micro-level space was present in the bonded interface. It is suggested that cobalt-chromium alloy for casting and acrylic resin for a thermoplastic base can be joined with no space in the bond interface by using LAMP joining without special surface processing. (J. Jpn. Soc. Laser Dent. 23:131 ~ 136, 2012 Reprint requests to Dr. NAITO) Key words= Laser joining, Dissimilar materials joining, Thermoplastic acrylic resin, Cobalt-chromium cast alloy キーワード=レーザー接合,異材接合,熱可塑性アクリル樹脂,コバルトクロム合金","PeriodicalId":187459,"journal":{"name":"Journal of Japanese Society for Laser Dentistry","volume":"23 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130613930","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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