Myconymphaea yatsukahoi is a fungus that has only been isolated once from a forest in the Sugadaira Research Station, Nagano, Japan. Over 20 y have passed since its first discovery but since then it has not been rediscovered. Here, we re-isolated M. yatsukahoi from the type locality and another location, Tambara Moor, Gunma, Japan. Sporophores of this species were detected by direct field observation in Sugadaira and by induction from soil from Tambara. We attempted to narrow down isolation sources of this species by investigating the excrements of Lithobiomorpha and Scolopendromorpha centipedes, which are frequently found in the two locations where the species is distributed. In both locations, we found M. yatsukahoi in the excrements of Lithobiomorpha but not Scolopendromorpha. Myconymphaea yatsukahoi appears to be a coprophilous fungus and the excrements of the predators living in soil may be promising isolation sources for understanding the hidden diversity of kickxellalean fungi.
{"title":"Revisiting the isolation source after the first discovery: <i>Myconymphaea yatsukahoi</i> on excrements of <i>Lithobiomorpha</i> (<i>Chilopoda</i>).","authors":"Yusuke Takashima, Mai Suyama, Kohei Yamamoto, Tomohiko Ri, Kazuhiko Narisawa, Yousuke Degawa","doi":"10.47371/mycosci.2022.04.003","DOIUrl":"https://doi.org/10.47371/mycosci.2022.04.003","url":null,"abstract":"<p><p><i>Myconymphaea yatsukahoi</i> is a fungus that has only been isolated once from a forest in the Sugadaira Research Station, Nagano, Japan. Over 20 y have passed since its first discovery but since then it has not been rediscovered. Here, we re-isolated <i>M. yatsukahoi</i> from the type locality and another location, Tambara Moor, Gunma, Japan. Sporophores of this species were detected by direct field observation in Sugadaira and by induction from soil from Tambara. We attempted to narrow down isolation sources of this species by investigating the excrements of <i>Lithobiomorpha</i> and <i>Scolopendromorpha</i> centipedes, which are frequently found in the two locations where the species is distributed. In both locations, we found <i>M. yatsukahoi</i> in the excrements of <i>Lithobiomorpha</i> but not <i>Scolopendromorpha</i>. <i>Myconymphaea yatsukahoi</i> appears to be a coprophilous fungus and the excrements of the predators living in soil may be promising isolation sources for understanding the hidden diversity of kickxellalean fungi.</p>","PeriodicalId":18780,"journal":{"name":"Mycoscience","volume":"63 4","pages":"176-180"},"PeriodicalIF":1.4,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/92/06/MYC-63-176.PMC10043566.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9387592","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Due to the high crude fiber content, straw of various crops is difficult to become a high quality forage resource. The degradation of cellulose in nature mainly depends on the cellulase secreted by microbes, which degrade cellulose into small molecular substances through chemical action, and the microbes that secrete cellulase mainly include some bacteria, fungi and actinomycetes, etc. The large and diverse microbial population contained in the mammalian gastrointestinal tract plays an important role in nutrient digestion. At present, many cellulose-degrading strains have been screened and obtained from animal digestive system and feces, such as Bacillus subtilis from the feces of Panda, Bacillus amyloliquefaciens from the cecum of goose. In this study, the fungal diversity was analysed in the fresh faeces of Tibetan sheep, Tibetan gazelle and Tibetan antelope in Qiangtang, Tibet. Results showed that the structure and species of gut fungi are different in three animals, which may be related to the different physiological functions among different animals, e.g., Tibetan antelope and Tibetan gazelle have stronger tolerance to rough feeding than Tibetan sheep. This study will lay a foundation for cellulose-degrading fungal development and provides technical support for improving rough feeding tolerance of Tibetan sheep.
{"title":"Characterization of fungal microbial diversity in Tibetan sheep, Tibetan gazelle and Tibetan antelope in the Qiangtang region of Tibet.","authors":"Zhenda Shang, Zhankun Tan, Qinghui Kong, Peng Shang, Honghui Wang, Wangjie Zhaxi, Ciren Zhaxi, Suozhu Liu","doi":"10.47371/mycosci.2022.05.004","DOIUrl":"https://doi.org/10.47371/mycosci.2022.05.004","url":null,"abstract":"<p><p>Due to the high crude fiber content, straw of various crops is difficult to become a high quality forage resource. The degradation of cellulose in nature mainly depends on the cellulase secreted by microbes, which degrade cellulose into small molecular substances through chemical action, and the microbes that secrete cellulase mainly include some bacteria, fungi and actinomycetes, etc. The large and diverse microbial population contained in the mammalian gastrointestinal tract plays an important role in nutrient digestion. At present, many cellulose-degrading strains have been screened and obtained from animal digestive system and feces, such as <i>Bacillus subtilis</i> from the feces of Panda, <i>Bacillus amyloliquefaciens</i> from the cecum of goose. In this study, the fungal diversity was analysed in the fresh faeces of Tibetan sheep, Tibetan gazelle and Tibetan antelope in Qiangtang, Tibet. Results showed that the structure and species of gut fungi are different in three animals, which may be related to the different physiological functions among different animals, e.g., Tibetan antelope and Tibetan gazelle have stronger tolerance to rough feeding than Tibetan sheep. This study will lay a foundation for cellulose-degrading fungal development and provides technical support for improving rough feeding tolerance of Tibetan sheep.</p>","PeriodicalId":18780,"journal":{"name":"Mycoscience","volume":"63 4","pages":"156-164"},"PeriodicalIF":1.4,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/0b/62/MYC-63-156.PMC10042320.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9387596","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.47371/mycosci.2022.08.002
Taiga Kasuya, Kentaro Hosaka, Makoto Kakishima
Puccinia caricis-smilacis, a new species of caricicolous rust fungus was described based on morphological characteristics and nuclear rDNA sequences from Japan. The heteroecious life cycle of P. caricis-smilacis was elucidated via field observations, inoculation experiments and molecular analyses. This rust fungus produces uredinial and telial stages on Carex fibrillosa, and spermogonial and aecial stages on Smilax china.
{"title":"<i>Puccinia caricis-smilacis</i>, a new caricicolous rust fungus producing spermogonial and aecial stages on <i>Smilax china</i> in Japan.","authors":"Taiga Kasuya, Kentaro Hosaka, Makoto Kakishima","doi":"10.47371/mycosci.2022.08.002","DOIUrl":"https://doi.org/10.47371/mycosci.2022.08.002","url":null,"abstract":"<p><p><i>Puccinia caricis-smilacis</i>, a new species of caricicolous rust fungus was described based on morphological characteristics and nuclear rDNA sequences from Japan. The heteroecious life cycle of <i>P. caricis-smilacis</i> was elucidated via field observations, inoculation experiments and molecular analyses. This rust fungus produces uredinial and telial stages on <i>Carex fibrillosa,</i> and spermogonial and aecial stages on <i>Smilax china</i>.</p>","PeriodicalId":18780,"journal":{"name":"Mycoscience","volume":"63 5","pages":"235-241"},"PeriodicalIF":1.4,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/82/4f/MYC-63-235.PMC10024967.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9387570","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A white-colored truffle Tuber japonicum, indigenous to Japan, is an ascomycetous ectomycorrhizal fungus. To clarify the physiological characteristics of this fungus, we investigated the influence of culture medium, temperature, and sources of nitrogen (N) and carbon (C) on the growth of five strains. Tuber japonicum strains grew better on malt extract and modified Melin-Norkrans medium, and showed peak growth at 20 °C or 25 °C. This fungus utilized inorganic (NH4+ and NO3-) and organic N sources (casamino acids, glutamine, peptone, urea, and yeast extract). Additionally, this fungus utilized various C sources, such as monosaccharide (arabinose, fructose, galactose, glucose, and mannose), disaccharide (maltose, sucrose, and trehalose), polysaccharide (dextrin and soluble starch), and sugar alcohol (mannitol). However, nutrient sources that promote growth and their effects on growth promotion widely varied among strains. This can result from the strain difference in enzyme activities involved in the assimilation and metabolism of these sources.
{"title":"Physiological characteristics of pure cultures of a white-colored truffle <i>Tuber japonicum</i>.","authors":"Shota Nakano, Akihiko Kinoshita, Keisuke Obase, Noritaka Nakamura, Hitomi Furusawa, Kyotaro Noguchi, Takashi Yamanaka","doi":"10.47371/mycosci.2022.01.002","DOIUrl":"https://doi.org/10.47371/mycosci.2022.01.002","url":null,"abstract":"<p><p>A white-colored truffle <i>Tuber japonicum</i>, indigenous to Japan, is an ascomycetous ectomycorrhizal fungus. To clarify the physiological characteristics of this fungus, we investigated the influence of culture medium, temperature, and sources of nitrogen (N) and carbon (C) on the growth of five strains. <i>Tuber japonicum</i> strains grew better on malt extract and modified Melin-Norkrans medium, and showed peak growth at 20 °C or 25 °C. This fungus utilized inorganic (NH<sub>4</sub> <sup>+</sup> and NO<sub>3</sub> <sup>-</sup>) and organic N sources (casamino acids, glutamine, peptone, urea, and yeast extract). Additionally, this fungus utilized various C sources, such as monosaccharide (arabinose, fructose, galactose, glucose, and mannose), disaccharide (maltose, sucrose, and trehalose), polysaccharide (dextrin and soluble starch), and sugar alcohol (mannitol). However, nutrient sources that promote growth and their effects on growth promotion widely varied among strains. This can result from the strain difference in enzyme activities involved in the assimilation and metabolism of these sources.</p>","PeriodicalId":18780,"journal":{"name":"Mycoscience","volume":"63 2","pages":"53-57"},"PeriodicalIF":1.4,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/b5/e3/MYC-63-053.PMC9999085.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9388787","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The frequent occurrence of mushroom poisoning cases in the wet season in Thailand has long been recognized but has never been quantitatively analyzed. This study aims to analyze mushroom poisoning cases in Thailand between 2003 and 2017 and focused on their association with the rainfall. The results revealed 22,571 cases and 106 deaths in this period. Cases were higher for females than males, adults than children, and agriculturists than people in other occupations. Cases were higher in the northeastern and northern regions than in the central, south, and east regions. There are strong effects of seasonality on mushroom poisoning cases. Over the 15-y period, 17,337 cases occurred in the wet season (May-Sep) compared with 5,234 cases in the dry season (Oct-Apr). The number of monthly poisoning cases were high in men, mature adults, agriculturists, people living in the rural areas and people living in the Northeastern and Northern provinces. Moreover, a strong positive correlation was shown between the number of monthly poisoning cases and the amount of monthly rainfall.
{"title":"Mushroom poisoning in Thailand between 2003 and 2017.","authors":"Sayanh Somrithipol, Umpawa Pinruan, Sujinda Sommai, Phongsawat Khamsuntorn, Janet Jennifer Luangsa-Ard","doi":"10.47371/mycosci.2022.08.003","DOIUrl":"https://doi.org/10.47371/mycosci.2022.08.003","url":null,"abstract":"<p><p>The frequent occurrence of mushroom poisoning cases in the wet season in Thailand has long been recognized but has never been quantitatively analyzed. This study aims to analyze mushroom poisoning cases in Thailand between 2003 and 2017 and focused on their association with the rainfall. The results revealed 22,571 cases and 106 deaths in this period. Cases were higher for females than males, adults than children, and agriculturists than people in other occupations. Cases were higher in the northeastern and northern regions than in the central, south, and east regions. There are strong effects of seasonality on mushroom poisoning cases. Over the 15-y period, 17,337 cases occurred in the wet season (May-Sep) compared with 5,234 cases in the dry season (Oct-Apr). The number of monthly poisoning cases were high in men, mature adults, agriculturists, people living in the rural areas and people living in the Northeastern and Northern provinces. Moreover, a strong positive correlation was shown between the number of monthly poisoning cases and the amount of monthly rainfall.</p>","PeriodicalId":18780,"journal":{"name":"Mycoscience","volume":"63 6","pages":"267-273"},"PeriodicalIF":1.4,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/60/49/MYC-63-267.PMC10042301.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9740635","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The N-terminal amino acid sequence of the Pleurotus sp. 90 kDa protein was in good agreement with the corresponding sequence of the glycoside hydrolase (GH) family 37 protein (trehalase) from P. ostreatus PC 15 v2.0. The length of the Pleurotus sp. trehalase gene was 2247 bp, encoding a protein of 749 amino acids with a predicted molecular mass of 81.2 kDa. The molecular mass of the recombinant enzyme was estimated to be about 117 kDa by SDS-PAGE. We found that the recombinant enzyme comprised an N-glycosylated sugar chain and that its optimum pH and temperature were 4.5 and 40 ºC, respectively. Moreover, this enzyme exhibited high activity against trehalose exclusively. We found that the enzyme is novel acid trehalase belonging to GH family 37.
{"title":"Novel acid trehalase belonging to glycoside hydrolase family 37 from <i>Pleurotus</i> sp.: cloning, expression and characterization.","authors":"Gaku Tsutsumi, Chikako Kuroki, Kengo Kamei, Mizuho Kusuda, Masami Nakazawa, Tatsuji Sakamoto, Mariko Ishikawa, Shinji Harada, Hitoshi Kobayashi, Kenji Ouchi, Satoshi Inatomi, Minoru Sakaguchi, Takeo Iwamoto, Mitsuhiro Ueda","doi":"10.47371/mycosci.2022.09.001","DOIUrl":"https://doi.org/10.47371/mycosci.2022.09.001","url":null,"abstract":"<p><p>The N-terminal amino acid sequence of the <i>Pleurotus</i> sp. 90 kDa protein was in good agreement with the corresponding sequence of the glycoside hydrolase (GH) family 37 protein (trehalase) from <i>P. ostreatus</i> PC 15 v2.0. The length of the <i>Pleurotus</i> sp. trehalase gene was 2247 bp, encoding a protein of 749 amino acids with a predicted molecular mass of 81.2 kDa. The molecular mass of the recombinant enzyme was estimated to be about 117 kDa by SDS-PAGE. We found that the recombinant enzyme comprised an <i>N</i>-glycosylated sugar chain and that its optimum pH and temperature were 4.5 and 40 ºC, respectively. Moreover, this enzyme exhibited high activity against trehalose exclusively. We found that the enzyme is novel acid trehalase belonging to GH family 37.</p>","PeriodicalId":18780,"journal":{"name":"Mycoscience","volume":"63 6","pages":"284-292"},"PeriodicalIF":1.4,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/05/2b/MYC-63-284.PMC10024966.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9740636","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.47371/mycosci.2021.10.004
Qin Na, Zewei Liu, Hui Zeng, Xianhao Cheng, Yupeng Ge
Only a few Crepidotus species have been previously reported from subalpine areas of China. Members of Crepidotus possessing an orange-yellow pileus are rare in China as well. Here, we describe Crepidotus yuanchui, a new species characterized by an orange-yellow pileus, ovoid basidiospores, and abundant cylindric to narrowly utriform cheilocystidia. This species is widely distributed above 2000 m asl in Yunnan Province. In addition, C. caspari, which is a distinct species based on its combined characteristics of smooth basidiospores and clamp connections, is newly recorded and detailed from subalpine China. The results of phylogenetic analyses of ITS + nLSU sequences based on maximum likelihood and Bayesian inference methods support the recognition of these two species. Photographs, descriptions, line drawings, and comparisons of related species are provided.
{"title":"<i>Crepidotus yuanchui</i> sp. nov. and <i>C. caspari</i> found in subalpine areas of China.","authors":"Qin Na, Zewei Liu, Hui Zeng, Xianhao Cheng, Yupeng Ge","doi":"10.47371/mycosci.2021.10.004","DOIUrl":"https://doi.org/10.47371/mycosci.2021.10.004","url":null,"abstract":"<p><p>Only a few <i>Crepidotus</i> species have been previously reported from subalpine areas of China. Members of <i>Crepidotus</i> possessing an orange-yellow pileus are rare in China as well. Here, we describe <i>Crepidotus yuanchui</i>, a new species characterized by an orange-yellow pileus, ovoid basidiospores, and abundant cylindric to narrowly utriform cheilocystidia. This species is widely distributed above 2000 m asl in Yunnan Province. In addition, <i>C. caspari</i>, which is a distinct species based on its combined characteristics of smooth basidiospores and clamp connections, is newly recorded and detailed from subalpine China. The results of phylogenetic analyses of ITS + nLSU sequences based on maximum likelihood and Bayesian inference methods support the recognition of these two species. Photographs, descriptions, line drawings, and comparisons of related species are provided.</p>","PeriodicalId":18780,"journal":{"name":"Mycoscience","volume":"63 1","pages":"1-11"},"PeriodicalIF":1.4,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/6f/d9/MYC-63-001.PMC10043943.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9742126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
We describe a new truffle species, Tuber torulosum, based on molecular and morphological analyses. This species forms a single globose ascospore per ascus, pale yellow in color, as do Japanese T. flavidosporum and Chinese T. turmericum and T. xanthomonosporum in the Japonicum clade of the Tuber phylogeny. However, it can be distinguished from them microscopically by its whitish tomentose mycelium that partially covers the ascoma surface and the mesh size of its spore ornamentation. Cystidia are moniliform and yellowish to reddish. Molecular phylogenetic analysis using the internal transcribed spacer and partial large subunit regions of ribosomal DNA also supports T. torulosum as a distinct species. On the basis of our results, we provide a key to species in the Japonicum clade.
{"title":"<i>Tuber torulosum</i>: A new truffle species decorated with moniliform cystidia from Japan.","authors":"Akihiko Kinoshita, Kohei Yamamoto, Toshiyuki Tainaka, Toshifumi Handa, Akiyoshi Yamada","doi":"10.47371/mycosci.2021.10.005","DOIUrl":"https://doi.org/10.47371/mycosci.2021.10.005","url":null,"abstract":"<p><p>We describe a new truffle species, <i>Tuber torulosum</i>, based on molecular and morphological analyses. This species forms a single globose ascospore per ascus, pale yellow in color, as do Japanese <i>T. flavidosporum</i> and Chinese <i>T. turmericum</i> and <i>T. xanthomonosporum</i> in the Japonicum clade of the <i>Tuber</i> phylogeny. However, it can be distinguished from them microscopically by its whitish tomentose mycelium that partially covers the ascoma surface and the mesh size of its spore ornamentation. Cystidia are moniliform and yellowish to reddish. Molecular phylogenetic analysis using the internal transcribed spacer and partial large subunit regions of ribosomal DNA also supports <i>T. torulosum</i> as a distinct species. On the basis of our results, we provide a key to species in the Japonicum clade.</p>","PeriodicalId":18780,"journal":{"name":"Mycoscience","volume":"63 1","pages":"26-32"},"PeriodicalIF":1.4,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/a2/b7/MYC-63-026.PMC10045829.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9742131","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.47371/mycosci.2022.02.002
Jun-Hong Dong, Ya-Xing Wu, Chang-Lin Zhao
Two new wood-inhabiting fungal species, Steccherinum hirsutum and S. yunnanense spp. nov., are proposed based on a combination of morphological features and molecular evidence. Sequences of internal transcribed spacer and large subunit region of nuculear ribosomal RNA gene of the studied samples were generated, and phylogenetic analyses were performed using maximum likelihood, maximum parsimony and bayesian inference methods. Steccherinum hirsutum is characterized by an annual growth habit, stipitate basidiocarps with scarlet to red, odontioid hymenial surface, a dimitic hyphal system with clamped generative hyphae negative in Melzer's reaction, and acyanophilous basidiospores measuring 2.5-3.5 × 1.5-2.5 µm. Steccherinum yunnanense is distinguished by resupinate basidiomata with odontioid hymenial surface, a dimitic hyphal system with clamped generative hyphae, strongly encrusted cystidia and ellipsoid, hyaline, thin-walled, smooth basidiospores (3.5-4.5 × 2-3.5 µm). The phylogenetic analyses comfirmed that two new species nest in Steccherinum, in the residual polyporoid clade.
{"title":"Two new species of <i>Steccherinum</i> (<i>Polyporales</i>, <i>Basidiomycota</i>) from southern China based on morphology and DNA sequence data.","authors":"Jun-Hong Dong, Ya-Xing Wu, Chang-Lin Zhao","doi":"10.47371/mycosci.2022.02.002","DOIUrl":"https://doi.org/10.47371/mycosci.2022.02.002","url":null,"abstract":"<p><p>Two new wood-inhabiting fungal species, <i>Steccherinum hirsutum</i> and <i>S. yunnanense</i> spp. nov., are proposed based on a combination of morphological features and molecular evidence. Sequences of internal transcribed spacer and large subunit region of nuculear ribosomal RNA gene of the studied samples were generated, and phylogenetic analyses were performed using maximum likelihood, maximum parsimony and bayesian inference methods. <i>Steccherinum hirsutum</i> is characterized by an annual growth habit, stipitate basidiocarps with scarlet to red, odontioid hymenial surface, a dimitic hyphal system with clamped generative hyphae negative in Melzer's reaction, and acyanophilous basidiospores measuring 2.5-3.5 × 1.5-2.5 µm. <i>Steccherinum yunnanense</i> is distinguished by resupinate basidiomata with odontioid hymenial surface, a dimitic hyphal system with clamped generative hyphae, strongly encrusted cystidia and ellipsoid, hyaline, thin-walled, smooth basidiospores (3.5-4.5 × 2-3.5 µm). The phylogenetic analyses comfirmed that two new species nest in <i>Steccherinum</i>, in the residual polyporoid clade.</p>","PeriodicalId":18780,"journal":{"name":"Mycoscience","volume":"63 2","pages":"65-72"},"PeriodicalIF":1.4,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/3b/8e/MYC-63-065.PMC10024968.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9382384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.47371/mycosci.2022.05.003
Yuri K Novozhilov, Ilya S Prikhodko, Nadezhda A Fedorova, Oleg N Shchepin, Vladimir I Gmoshinskiy, Martin Schnittler
A new species of Lamproderma (Myxomycetes), described herein as L. vietnamense, was recovered in the field on ground litter from mountain subtropical forests (Phia Oắc - Phia Đén National Park) of northern Vietnam. Morphological details were examined by light and scanning electron microscopy. The species is characterized by a distinct and unique combination of morphological features, including a bright blue, shiny and very thin membranous peridium, a small dome-shaped columella, rigid, straight, branched, brown capillitial threads which gradually become pale at the periphery and finally colorless at the tips and small-meshed, banded-reticulate spores with 9-12 meshes across the spore diameter and solid walls without perforations 0.3-0.5 µm high. The stability of the taxonomic characters of L. vietnamense is supported by two well-developed collections found in 2018 and 2019. Partial sequences of three molecular markers (SSU, EF1α, COI) for both collections are identical. A two-gene phylogeny of the first two markers displays the two known accessions as a well-separated entity and indicates affinity of the new species with L. columbinum (the type taxon of the genus), L. violaceum, and several nivicolous Lamproderma species.
{"title":"<i>Lamproderma vietnamense</i>: a new species of myxomycetes with reticulate spores from Phia Oắc - Phia Đén National Park (northern Vietnam) supported by molecular phylogeny and morphological analysis.","authors":"Yuri K Novozhilov, Ilya S Prikhodko, Nadezhda A Fedorova, Oleg N Shchepin, Vladimir I Gmoshinskiy, Martin Schnittler","doi":"10.47371/mycosci.2022.05.003","DOIUrl":"https://doi.org/10.47371/mycosci.2022.05.003","url":null,"abstract":"<p><p>A new species of <i>Lamproderma</i> (<i>Myxomycetes</i>), described herein as <i>L. vietnamense</i>, was recovered in the field on ground litter from mountain subtropical forests (Phia Oắc - Phia Đén National Park) of northern Vietnam. Morphological details were examined by light and scanning electron microscopy. The species is characterized by a distinct and unique combination of morphological features, including a bright blue, shiny and very thin membranous peridium, a small dome-shaped columella, rigid, straight, branched, brown capillitial threads which gradually become pale at the periphery and finally colorless at the tips and small-meshed, banded-reticulate spores with 9-12 meshes across the spore diameter and solid walls without perforations 0.3-0.5 µm high. The stability of the taxonomic characters of <i>L. vietnamense</i> is supported by two well-developed collections found in 2018 and 2019. Partial sequences of three molecular markers (SSU, EF1α, COI) for both collections are identical. A two-gene phylogeny of the first two markers displays the two known accessions as a well-separated entity and indicates affinity of the new species with <i>L. columbinum</i> (the type taxon of the genus), <i>L. violaceum</i>, and several nivicolous <i>Lamproderma</i> species.</p>","PeriodicalId":18780,"journal":{"name":"Mycoscience","volume":"63 4","pages":"149-155"},"PeriodicalIF":1.4,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/52/a7/MYC-63-149.PMC10043568.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9387595","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}