Pub Date : 2024-06-01DOI: 10.46488/nept.2024.v23i02.031
Srikanth Satish Kumar Darapu, S. Vindula
Research on the impact of alkali contamination on the swelling behavior of red earth in the Visakhapatnam region has been notably limited. Therefore, this study aims to investigate the effects of alkali (NaOH) contamination on the swelling characteristics of the region’s red earth. The red earth of this region was found to be a well-graded sandy soil with 81% sand and 18% fines. X-ray diffraction studies showed that this region’s red earth mainly consists of quartz, kaolinite, and hematite. The soil is inherently non-swelling. However, the free swell tests showed considerable swell under contamination of NaOH solutions of various normalities (0.05, 0.1, 1, 2, and 4N). One-dimensional consolidation tests have shown that the swell increased with the concentration of the NaOH solution and with the duration of the interaction. Red earth exhibited 'an equilibrium swelling' of 5.6, 10, 15, 17, and 20% when contaminated with 0.05, 0.1, 1, 2, and 4N NaOH solutions, respectively. XRD studies revealed that the red earth sample contaminated with even 0.05N NaOH solution and cured for 56 days exhibited the formation of zeolites analcime and natrolite. Silicate minerals like paragonite and ussingite were also formed along with the zeolites. N-A-S-H compounds, hydrosodalites, and zeolites like super hydrated natrolite, zeolite SSZ16, and zeolite ZK-14 were formed at higher normalities of NaOH after a curing period of 56 days, which caused increased swell. The research demonstrated that the formation of zeolites resulting from the alkali contamination led to swelling in the red earth.
{"title":"Assessment of the Swelling Behavior of NaOH-Contaminated Red Earth in the Visakhapatnam Region of India Using X-ray Diffraction Analysis","authors":"Srikanth Satish Kumar Darapu, S. Vindula","doi":"10.46488/nept.2024.v23i02.031","DOIUrl":"https://doi.org/10.46488/nept.2024.v23i02.031","url":null,"abstract":"Research on the impact of alkali contamination on the swelling behavior of red earth in the Visakhapatnam region has been notably limited. Therefore, this study aims to investigate the effects of alkali (NaOH) contamination on the swelling characteristics of the region’s red earth. The red earth of this region was found to be a well-graded sandy soil with 81% sand and 18% fines. X-ray diffraction studies showed that this region’s red earth mainly consists of quartz, kaolinite, and hematite. The soil is inherently non-swelling. However, the free swell tests showed considerable swell under contamination of NaOH solutions of various normalities (0.05, 0.1, 1, 2, and 4N). One-dimensional consolidation tests have shown that the swell increased with the concentration of the NaOH solution and with the duration of the interaction. Red earth exhibited 'an equilibrium swelling' of 5.6, 10, 15, 17, and 20% when contaminated with 0.05, 0.1, 1, 2, and 4N NaOH solutions, respectively. XRD studies revealed that the red earth sample contaminated with even 0.05N NaOH solution and cured for 56 days exhibited the formation of zeolites analcime and natrolite. Silicate minerals like paragonite and ussingite were also formed along with the zeolites. N-A-S-H compounds, hydrosodalites, and zeolites like super hydrated natrolite, zeolite SSZ16, and zeolite ZK-14 were formed at higher normalities of NaOH after a curing period of 56 days, which caused increased swell. The research demonstrated that the formation of zeolites resulting from the alkali contamination led to swelling in the red earth.","PeriodicalId":18783,"journal":{"name":"Nature Environment and Pollution Technology","volume":"142 16","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141281125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-06-01DOI: 10.46488/nept.2024.v23i02.041
Ishar, Paulina Taba, Fahruddin
Various wastes can be utilized to produce activated carbon, one of the wastes that can be utilized is nutmeg shell (Myristica fragrans). Activated carbon from nutmeg shells (Myristica fragrans) was used in this study to reduce the content of Pb(II) and Cu(II) ions in liquid waste. This research utilized the adsorption method with the batch system to determine the optimum contact time, optimum pH, and adsorption capacity. The characterization of activated carbon was done by Scanning Electron Microscopy (SEM) and Surface Area Analyzers (SAA). The content of Pb(II) and Cu(II) ions in the filtrate after adsorption was analyzed using an atomic absorption spectrophotometer (AAS). The results of SEM analysis showed that the carbon surface was cleaner and had more open pores after the activation process than before activation. The carbon surface area is 19.6243 m2.g-1. From the results of AAS analysis, the optimum time and pH for Pb(II) and Cu(II) ions was 40 min at pH 5 and 70 min at pH 4. With the Freundlich isotherm method, the adsorption capacity of the adsorbent for Pb(II) ions was 9.6028 mg.g-1 and Cu(II) ions was 0.035 mg.g-1, and the adsorption effectiveness on liquid waste for Pb and Cu metals was 1.9454 mg.g-1 and 0.4251 mg.g-1, respectively. The results showed that activated carbon from the nutmeg shell (Myristica fragrans) was able to reduce the levels of Pb(II) and Cu(II) ions in liquid waste.
{"title":"Effectiveness of Activated Carbon from Nutmeg Shell (Myristica fragrans) Waste as Adsorbent for Metal Ions Pb(II) and Cu(II) in Liquid Waste","authors":"Ishar, Paulina Taba, Fahruddin","doi":"10.46488/nept.2024.v23i02.041","DOIUrl":"https://doi.org/10.46488/nept.2024.v23i02.041","url":null,"abstract":"Various wastes can be utilized to produce activated carbon, one of the wastes that can be utilized is nutmeg shell (Myristica fragrans). Activated carbon from nutmeg shells (Myristica fragrans) was used in this study to reduce the content of Pb(II) and Cu(II) ions in liquid waste. This research utilized the adsorption method with the batch system to determine the optimum contact time, optimum pH, and adsorption capacity. The characterization of activated carbon was done by Scanning Electron Microscopy (SEM) and Surface Area Analyzers (SAA). The content of Pb(II) and Cu(II) ions in the filtrate after adsorption was analyzed using an atomic absorption spectrophotometer (AAS). The results of SEM analysis showed that the carbon surface was cleaner and had more open pores after the activation process than before activation. The carbon surface area is 19.6243 m2.g-1. From the results of AAS analysis, the optimum time and pH for Pb(II) and Cu(II) ions was 40 min at pH 5 and 70 min at pH 4. With the Freundlich isotherm method, the adsorption capacity of the adsorbent for Pb(II) ions was 9.6028 mg.g-1 and Cu(II) ions was 0.035 mg.g-1, and the adsorption effectiveness on liquid waste for Pb and Cu metals was 1.9454 mg.g-1 and 0.4251 mg.g-1, respectively. The results showed that activated carbon from the nutmeg shell (Myristica fragrans) was able to reduce the levels of Pb(II) and Cu(II) ions in liquid waste.","PeriodicalId":18783,"journal":{"name":"Nature Environment and Pollution Technology","volume":"133 9","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141281688","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-06-01DOI: 10.46488/nept.2024.v23i02.037
Christsam Joy S. Jaspe-Santander, I. Tabañag
The study presented aims to find the most appropriate climate dataset for the data-scarce Jalaur River Basin (JRB), Iloilo, Philippines, by evaluating the statistical performance of five rainfall datasets (APHRODITE, CPC NOAA, ERA5, SA-OBS, and PGF-V3) with resolutions of 0.25° and 0.5° having a time domain of 1981 to 2005. Bilinear interpolation implemented through Climate Data Operator (CDO) was used to extract and process grid climate datasets with Linear scaling as bias correction to minimize product simulation uncertainties. The datasets were compared to the lone meteorological station nearest to JRB investigated at monthly and annual timescales using six statistical metrics, namely, Pearson’s correlation coefficient (r), coefficient of determination (R2), modified index of agreement (d1), Kling-Gupta efficiency, Nash-Sutcliffe efficiency (NSE), and RMSE-observations standard deviation ratio (RSR). The results indicate a strong positive correlation with the observed data for both rainfall and temperature (r > 0.8; R2, d1 > 0.80). Although graphical observation shows an underestimation of rainfall, goodness-of-fit values indicate very good model performance (NSE, KGE > 0.75; RSR < 0.50). In terms of temperature, variable responses are observed with significant overestimation for maximum temperature and underestimation for minimum temperature. SA-OBS proved to be the best-performing dataset, followed by ERA5 and PGF-V3. These key findings supply useful information in deciding the most appropriate gridded climate dataset for hydrometeorological investigation in the JRB and could enhance the regional representation of global datasets.
{"title":"Statistical Performance of Gridded Rainfall Datasets Over Ungauged Jalaur River Basin, Philippines","authors":"Christsam Joy S. Jaspe-Santander, I. Tabañag","doi":"10.46488/nept.2024.v23i02.037","DOIUrl":"https://doi.org/10.46488/nept.2024.v23i02.037","url":null,"abstract":"The study presented aims to find the most appropriate climate dataset for the data-scarce Jalaur River Basin (JRB), Iloilo, Philippines, by evaluating the statistical performance of five rainfall datasets (APHRODITE, CPC NOAA, ERA5, SA-OBS, and PGF-V3) with resolutions of 0.25° and 0.5° having a time domain of 1981 to 2005. Bilinear interpolation implemented through Climate Data Operator (CDO) was used to extract and process grid climate datasets with Linear scaling as bias correction to minimize product simulation uncertainties. The datasets were compared to the lone meteorological station nearest to JRB investigated at monthly and annual timescales using six statistical metrics, namely, Pearson’s correlation coefficient (r), coefficient of determination (R2), modified index of agreement (d1), Kling-Gupta efficiency, Nash-Sutcliffe efficiency (NSE), and RMSE-observations standard deviation ratio (RSR). The results indicate a strong positive correlation with the observed data for both rainfall and temperature (r > 0.8; R2, d1 > 0.80). Although graphical observation shows an underestimation of rainfall, goodness-of-fit values indicate very good model performance (NSE, KGE > 0.75; RSR < 0.50). In terms of temperature, variable responses are observed with significant overestimation for maximum temperature and underestimation for minimum temperature. SA-OBS proved to be the best-performing dataset, followed by ERA5 and PGF-V3. These key findings supply useful information in deciding the most appropriate gridded climate dataset for hydrometeorological investigation in the JRB and could enhance the regional representation of global datasets.","PeriodicalId":18783,"journal":{"name":"Nature Environment and Pollution Technology","volume":"36 9","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141276209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-06-01DOI: 10.46488/nept.2024.v23i02.010
Sukjailin Ryntathiang, Meguovilie Sachu, M. B. Syiem
This study aimed to check how herbicide 2,4-dichlorophenoxyacetic acid (2,4-D) affects the production of EPS and its composition, growth, and biomass, as well as morphology in a cyanobacterial species isolated from a rice field in Meghalaya, India. Compared to the control cells, the growth of the organism measured in terms of chlorophyll concentration increased after being exposed to 10 and 20 ppm 2,4-D. However, cultures treated with 30 and 40 ppm experienced a decrease in their growth. Likewise, the biomass content of the organism experienced a minuscule increase in content upon exposure to 10 and 20 ppm 2,4-D but was compromised upon exposure to higher doses. When exposed to 10 ppm, the total EPS content, which includes the RPS and CPS content, showed a substantial increase. Maximum EPS production was seen at 20 ppm 2,4-D. However, exposure to 30 and 40 ppm 2,4-D, EPS production in the organism experienced a significant reduction, respectively. All components of EPS, such as uronic acid, neutral sugar, and proteins, individually showed an increase in 10 and 20 ppm 2, 4-D. A similar trend was seen in the organism’s bio-flocculating activity, which increased when exposed to 10 and 20 ppm, respectively. However, this activity in cells exposed to 30 and 40 ppm 2,4-D was severely reduced. Not only the content of EPS but the rate of EPS production was also enhanced in lower concentrations of 2,4-D. Although exposure to 30 ppm 2,4-D, the rate of EPS production was not significantly compromised, 40 ppm exposure adversely affected the rate of EPS production. Furthermore, visualization using scanning electron microscopy revealed the morphological changes induced by the herbicide 2,4-D.
{"title":"Response and Tolerance of Cyanobacterial Exopolysaccharides to Rice Field Herbicide 2,4-D","authors":"Sukjailin Ryntathiang, Meguovilie Sachu, M. B. Syiem","doi":"10.46488/nept.2024.v23i02.010","DOIUrl":"https://doi.org/10.46488/nept.2024.v23i02.010","url":null,"abstract":"This study aimed to check how herbicide 2,4-dichlorophenoxyacetic acid (2,4-D) affects the production of EPS and its composition, growth, and biomass, as well as morphology in a cyanobacterial species isolated from a rice field in Meghalaya, India. Compared to the control cells, the growth of the organism measured in terms of chlorophyll concentration increased after being exposed to 10 and 20 ppm 2,4-D. However, cultures treated with 30 and 40 ppm experienced a decrease in their growth. Likewise, the biomass content of the organism experienced a minuscule increase in content upon exposure to 10 and 20 ppm 2,4-D but was compromised upon exposure to higher doses. When exposed to 10 ppm, the total EPS content, which includes the RPS and CPS content, showed a substantial increase. Maximum EPS production was seen at 20 ppm 2,4-D. However, exposure to 30 and 40 ppm 2,4-D, EPS production in the organism experienced a significant reduction, respectively. All components of EPS, such as uronic acid, neutral sugar, and proteins, individually showed an increase in 10 and 20 ppm 2, 4-D. A similar trend was seen in the organism’s bio-flocculating activity, which increased when exposed to 10 and 20 ppm, respectively. However, this activity in cells exposed to 30 and 40 ppm 2,4-D was severely reduced. Not only the content of EPS but the rate of EPS production was also enhanced in lower concentrations of 2,4-D. Although exposure to 30 ppm 2,4-D, the rate of EPS production was not significantly compromised, 40 ppm exposure adversely affected the rate of EPS production. Furthermore, visualization using scanning electron microscopy revealed the morphological changes induced by the herbicide 2,4-D.","PeriodicalId":18783,"journal":{"name":"Nature Environment and Pollution Technology","volume":"20 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141280519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-06-01DOI: 10.46488/nept.2024.v23i02.054
Zaid Raad Abbas, Aqeel Mohammed Majeed Al-Ezee, S. H. Authman, M. Shafeeq
Loam examples were gathered through the 2020-2021 rising periods, and the following measurements were made: Viable bacterial count by reducing root colonization. The outcomes of reviewing the impact of the fungicide Benomyl on development and viable microflora count revealed that the highest microbial count was in Al-Madaein 80 ×103 CFU/mL was recorded ., and the lowest count was 60 ×103 CFU/mL for the Aushtar area, The microbial viable count values for the affected microorganisms with Benomyl were decrease to 27×103 and 65 × 103 CFU/mL respectively. Those consequences specify that Benomyl has a robust choosiness contrary to microflora, especially when compared to the benomyl effect as folded dose, the microflora l count decreases to 25 ×103 CFU /mL in the Aushtar area and increases to 60 ×103 CFU/mL in Al-Madaein area. Whereas the study estimated the level of eight elements in soil (Mn, Fe, Cu, Zn, NO3, P, K, and NH4) cultured with Cyperus rotundus L. Which mentioned the effect of benomyl on these levels after three days of treatment. Mn concentration ranged between 5.96 to 9.11 ppm, while after fungicide benomyl, it decreased to 5.63 -6.53 ppm similar results were observed for other elements. The highest affected element was Mn in the Aushtar area. Those consequences designate that Benomyl has a stout fussiness in contrast to soil nutrients. The greatness of benomyl impacts on loam ingredients and procedures were minor, qualified to impact on mycorrhizal root foundation (reduction through benomyl).
{"title":"The Passive Environmental Effect of the Fungicide Benomyl on Soil Promoting Bacteria and Concentration of Some Important Soil Elements","authors":"Zaid Raad Abbas, Aqeel Mohammed Majeed Al-Ezee, S. H. Authman, M. Shafeeq","doi":"10.46488/nept.2024.v23i02.054","DOIUrl":"https://doi.org/10.46488/nept.2024.v23i02.054","url":null,"abstract":"Loam examples were gathered through the 2020-2021 rising periods, and the following measurements were made: Viable bacterial count by reducing root colonization. The outcomes of reviewing the impact of the fungicide Benomyl on development and viable microflora count revealed that the highest microbial count was in Al-Madaein 80 ×103 CFU/mL was recorded ., and the lowest count was 60 ×103 CFU/mL for the Aushtar area, The microbial viable count values for the affected microorganisms with Benomyl were decrease to 27×103 and 65 × 103 CFU/mL respectively. Those consequences specify that Benomyl has a robust choosiness contrary to microflora, especially when compared to the benomyl effect as folded dose, the microflora l count decreases to 25 ×103 CFU /mL in the Aushtar area and increases to 60 ×103 CFU/mL in Al-Madaein area. Whereas the study estimated the level of eight elements in soil (Mn, Fe, Cu, Zn, NO3, P, K, and NH4) cultured with Cyperus rotundus L. Which mentioned the effect of benomyl on these levels after three days of treatment. Mn concentration ranged between 5.96 to 9.11 ppm, while after fungicide benomyl, it decreased to 5.63 -6.53 ppm similar results were observed for other elements. The highest affected element was Mn in the Aushtar area. Those consequences designate that Benomyl has a stout fussiness in contrast to soil nutrients. The greatness of benomyl impacts on loam ingredients and procedures were minor, qualified to impact on mycorrhizal root foundation (reduction through benomyl).","PeriodicalId":18783,"journal":{"name":"Nature Environment and Pollution Technology","volume":"45 6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141275105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-06-01DOI: 10.46488/nept.2024.v23i02.015
Kiran Bishnoi, P. Rani, Minakshi Karwal, N. Bishnoi
In the present study, biodegradation of polycyclic aromatic hydrocarbons (PAHs) was examined using two fungal strains, namely P. chrysosporium and P. citrinum, isolated from locally contaminated soil. These two fungal strains were compared based on degradation properties under standardized conditions (pH 7.0, temperature 30oC, carbon source yeast extract) using PAH sole and a mixture of five different PAHs. In liquid media, PAH degradation was higher as compared to spiked soil by P. chrysosporium, followed by P. citrinum. In liquid culture, maximum degradation was 96.13% phenanathrene, 86.34% fluoranthene, 72.75% pyrene, 52.25% chrysene, and 40.16% benzo(a)pyrene by P. chrysosporium. PAH degradation in spiked soil was 78.5% phenanthrene, 65.91% fluoranthene, 61.73% pyrene, 48.2% chrysene, and 26.82% benzo(a)pyrene within 28 days by P. chrysosporium. Both local fungal isolates showed potential for degradation of PAHs alone and in PAH mixtures.
本研究利用从当地受污染土壤中分离出的两种真菌菌株，即 P. chrysosporium 和 P. citrinum，对多环芳烃（PAHs）的生物降解进行了研究。在标准化条件下（pH 值 7.0，温度 30oC，碳源为酵母提取物），比较了这两种真菌菌株对单一种 PAH 和五种不同 PAH 混合物的降解特性。在液体培养基中，P. chrysosporium 的多环芳烃降解率高于加标土壤，其次是 P. citrinum。在液体培养基中，蛹虫草菌对苯并芘、氟蒽、芘、菊烯和苯并（a）芘的最大降解率分别为 96.13%、86.34%、72.75%、52.25% 和 40.16%。在 28 天内，蛹虫草真菌对土壤中多环芳烃的降解率分别为 78.5%菲、65.91%荧蒽、61.73%芘、48.2%菊烯和 26.82%苯并(a)芘。两种本地真菌分离物都显示出降解单独的多环芳烃和多环芳烃混合物的潜力。
{"title":"PAHs Biodegradation by Locally Isolated Phanerochaete chrysosporium and Penicillium citrinum from Liquid and Spiked Soil","authors":"Kiran Bishnoi, P. Rani, Minakshi Karwal, N. Bishnoi","doi":"10.46488/nept.2024.v23i02.015","DOIUrl":"https://doi.org/10.46488/nept.2024.v23i02.015","url":null,"abstract":"In the present study, biodegradation of polycyclic aromatic hydrocarbons (PAHs) was examined using two fungal strains, namely P. chrysosporium and P. citrinum, isolated from locally contaminated soil. These two fungal strains were compared based on degradation properties under standardized conditions (pH 7.0, temperature 30oC, carbon source yeast extract) using PAH sole and a mixture of five different PAHs. In liquid media, PAH degradation was higher as compared to spiked soil by P. chrysosporium, followed by P. citrinum. In liquid culture, maximum degradation was 96.13% phenanathrene, 86.34% fluoranthene, 72.75% pyrene, 52.25% chrysene, and 40.16% benzo(a)pyrene by P. chrysosporium. PAH degradation in spiked soil was 78.5% phenanthrene, 65.91% fluoranthene, 61.73% pyrene, 48.2% chrysene, and 26.82% benzo(a)pyrene within 28 days by P. chrysosporium. Both local fungal isolates showed potential for degradation of PAHs alone and in PAH mixtures.","PeriodicalId":18783,"journal":{"name":"Nature Environment and Pollution Technology","volume":"59 8","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141275792","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-06-01DOI: 10.46488/nept.2024.v23i02.017
Siriorn Boonyawanich, P. Prommeenate, S. Oaew, N. Pisutpaisal, S. Haosagul
Hydrogen sulfide (H2S) is highly corrosive to electric generators, which is the main problem of biogas utilization. The industrial scale of the biofilter system relies on the performance of sulfide-oxidizing bacteria (SOB) via the activity of sulfur oxidation (soxABXYZ) and flavocytochrome sulfide dehydrogenase (fccAB) enzymes to reduce to a concentration below 100 ppm before using in industrial machinery. The main purpose of this research is to investigate the SOB community in full-scale H2S removal and their gene expression (fccAB and soxABXYZ) associated with H2S elimination efficiency. In this study, SOB communities were obtained from 2 sampling sites of the full-scale biofilter of palm oil factory (PPG), comprising starting sludge (PPG1) and recirculating sludge (PPG2). The abundance of SOB strains was examined by next-generation sequencing analysis (NGS) based on the 16S rRNA gene. The changes in the expression of genes involved in sulfur oxidation, namely soxABXYZ, and fccAB, between the 2 sampling sites were evaluated by using a comparative genomic hybridization (CGH) microarray. The results indicate that the high abundance of SOB genera that could play a vital role in biofilters belonged mainly to Sulfurovum, Paracoccus, Acidihalobacter, Acidithiobacillus, Thioalkalispira, Thiofaba, Caldisericum, Bacillus, were rapidly increased in the biofilter tank. Interestingly, expressions of soxAXYZ gene cluster at PPG2 were increased in Paracoccus pantotrophus J40 and Paracoccus alkenifer DSM 11593 for 1.1188 and 1.0518-fold, respectively, while in Acidihalobacter prosperus F5, the expression of fccAB genes was up to 1.3704 fold in comparison with PPG1. Increasing both relative abundance and gene expressions at PPG2 were correlated with 95% H2S removal efficiency. Hence, stabilization of the SOB microbiome is vital to H2S removal in industrial-scale biogas applications.
{"title":"Hydrogen Sulfide Oxidizing Microbiome in Biogas-Stream Fed Biofilter in Palm Oil Factory","authors":"Siriorn Boonyawanich, P. Prommeenate, S. Oaew, N. Pisutpaisal, S. Haosagul","doi":"10.46488/nept.2024.v23i02.017","DOIUrl":"https://doi.org/10.46488/nept.2024.v23i02.017","url":null,"abstract":"Hydrogen sulfide (H2S) is highly corrosive to electric generators, which is the main problem of biogas utilization. The industrial scale of the biofilter system relies on the performance of sulfide-oxidizing bacteria (SOB) via the activity of sulfur oxidation (soxABXYZ) and flavocytochrome sulfide dehydrogenase (fccAB) enzymes to reduce to a concentration below 100 ppm before using in industrial machinery. The main purpose of this research is to investigate the SOB community in full-scale H2S removal and their gene expression (fccAB and soxABXYZ) associated with H2S elimination efficiency. In this study, SOB communities were obtained from 2 sampling sites of the full-scale biofilter of palm oil factory (PPG), comprising starting sludge (PPG1) and recirculating sludge (PPG2). The abundance of SOB strains was examined by next-generation sequencing analysis (NGS) based on the 16S rRNA gene. The changes in the expression of genes involved in sulfur oxidation, namely soxABXYZ, and fccAB, between the 2 sampling sites were evaluated by using a comparative genomic hybridization (CGH) microarray. The results indicate that the high abundance of SOB genera that could play a vital role in biofilters belonged mainly to Sulfurovum, Paracoccus, Acidihalobacter, Acidithiobacillus, Thioalkalispira, Thiofaba, Caldisericum, Bacillus, were rapidly increased in the biofilter tank. Interestingly, expressions of soxAXYZ gene cluster at PPG2 were increased in Paracoccus pantotrophus J40 and Paracoccus alkenifer DSM 11593 for 1.1188 and 1.0518-fold, respectively, while in Acidihalobacter prosperus F5, the expression of fccAB genes was up to 1.3704 fold in comparison with PPG1. Increasing both relative abundance and gene expressions at PPG2 were correlated with 95% H2S removal efficiency. Hence, stabilization of the SOB microbiome is vital to H2S removal in industrial-scale biogas applications.","PeriodicalId":18783,"journal":{"name":"Nature Environment and Pollution Technology","volume":"22 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141280364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-06-01DOI: 10.46488/nept.2024.v23i02.056
E. E. Salman, A. Akol, J. S. A. Hamza, A. Naje
In this study, the AquaCrop model was employed to analyze the impact of projected future climate changes on the water usage and biomass production of potato crops in Babylon, Iraq, under varying irrigation methods. The irrigation techniques evaluated included sprinkler irrigation, surface drip irrigation, and subsurface drip irrigation at depths of 10 cm and 20 cm. The study involved simulating and forecasting conditions for the year 2050, comparing them to current conditions. The model measured and predicted the evapotranspiration (ETa) and actual biomass of potato crops for 2050 using the RCP 8.5 scenarios, which outline different trajectories for greenhouse gas emissions. The AquaCrop model was calibrated and validated using statistical measures such as the R2, RMSE, CV, EF, and D, achieving a 99% accuracy level in its performance. The findings suggest that using drip irrigation systems and applying the AquaCrop model significantly mitigates the adverse effects of environmental stress on desert soils and enhances sustainable agricultural practices in arid regions.
{"title":"Implementation of the AquaCrop Model for Forecasting the Effects of Climate Change on Water Consumption and Potato Yield Under Various Irrigation Techniques","authors":"E. E. Salman, A. Akol, J. S. A. Hamza, A. Naje","doi":"10.46488/nept.2024.v23i02.056","DOIUrl":"https://doi.org/10.46488/nept.2024.v23i02.056","url":null,"abstract":"In this study, the AquaCrop model was employed to analyze the impact of projected future climate changes on the water usage and biomass production of potato crops in Babylon, Iraq, under varying irrigation methods. The irrigation techniques evaluated included sprinkler irrigation, surface drip irrigation, and subsurface drip irrigation at depths of 10 cm and 20 cm. The study involved simulating and forecasting conditions for the year 2050, comparing them to current conditions. The model measured and predicted the evapotranspiration (ETa) and actual biomass of potato crops for 2050 using the RCP 8.5 scenarios, which outline different trajectories for greenhouse gas emissions. The AquaCrop model was calibrated and validated using statistical measures such as the R2, RMSE, CV, EF, and D, achieving a 99% accuracy level in its performance. The findings suggest that using drip irrigation systems and applying the AquaCrop model significantly mitigates the adverse effects of environmental stress on desert soils and enhances sustainable agricultural practices in arid regions.","PeriodicalId":18783,"journal":{"name":"Nature Environment and Pollution Technology","volume":"57 7","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141280966","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-06-01DOI: 10.46488/nept.2024.v23i02.039
S. Sathish, A. Saravanan, R. Suresh, K. Saranya, R. Sarweswaran, G. Balaji, S. Seralathan
This study focuses on converting fruit waste into usable clean energy by an innovative, cost-effective anaerobic biodigester. The biodigester is designed to anaerobically digest various fruit wastes and starter inoculums of cow dung that are locally obtained. A batch vertical digester of 1000 liters capacity built of fiber with a phonematic agitator positioned in the center is used to improve mixing. The retention time is 30 days with a substrate of banana peels co-digested with mango and papaya peels individually in the ratio of 50:50. The combined wastes generated the biogas and the total quantity of biogas generated for all combined wastes over 21 days varies between 530L/day and 480L/day respectively. In this work, banana and mango peel (waste/water) split 50:50 gives a peak yield of 530L/day. The average ambient temperatures are kept in the range of 25°C to 35°C (i.e., mesophilic range). The pH range of 6.4 to 7.8 is consistently maintained and seems to be stable. Therefore, this proposed anaerobic digester would reduce the disposal of solid waste, and it is cost-effective. After cleaning, it is observed that the combined peels of bananas and papaya contained 91.95% of the estimated biogas and methane, which can be used to solve energy issues such as electricity production and cooking purposes.
{"title":"Experimental Analysis of Anaerobic Co-digestion: Potential of Fruit Wastes","authors":"S. Sathish, A. Saravanan, R. Suresh, K. Saranya, R. Sarweswaran, G. Balaji, S. Seralathan","doi":"10.46488/nept.2024.v23i02.039","DOIUrl":"https://doi.org/10.46488/nept.2024.v23i02.039","url":null,"abstract":"This study focuses on converting fruit waste into usable clean energy by an innovative, cost-effective anaerobic biodigester. The biodigester is designed to anaerobically digest various fruit wastes and starter inoculums of cow dung that are locally obtained. A batch vertical digester of 1000 liters capacity built of fiber with a phonematic agitator positioned in the center is used to improve mixing. The retention time is 30 days with a substrate of banana peels co-digested with mango and papaya peels individually in the ratio of 50:50. The combined wastes generated the biogas and the total quantity of biogas generated for all combined wastes over 21 days varies between 530L/day and 480L/day respectively. In this work, banana and mango peel (waste/water) split 50:50 gives a peak yield of 530L/day. The average ambient temperatures are kept in the range of 25°C to 35°C (i.e., mesophilic range). The pH range of 6.4 to 7.8 is consistently maintained and seems to be stable. Therefore, this proposed anaerobic digester would reduce the disposal of solid waste, and it is cost-effective. After cleaning, it is observed that the combined peels of bananas and papaya contained 91.95% of the estimated biogas and methane, which can be used to solve energy issues such as electricity production and cooking purposes.","PeriodicalId":18783,"journal":{"name":"Nature Environment and Pollution Technology","volume":"53 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141280986","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-06-01DOI: 10.46488/nept.2024.v23i02.052
E. Ogbuene, A. M. Oroke, C. T. Eze, E. Etuk, O. G. Aloh, F. E. Achoru, J. C. Ogbuka, O. J. Okolo, A. V. Ozorme, C. J. Ibekwe, C. A. Eze, S. Akatakpo
Studies have emphasized that the presence of heavy metals in freshwater fish represents a global public health issue. Nigeria, being a developing nation with less emphasis on the quality of seafood consumed by the residents, ranks this study very vital. The policy implication of this study is the advancement of a healthy population in contemporary Nigeria. Hence, this study assessed heavy metal concentration in two fish species, Clarias gariepinus (Catfish) and Oreochromis niloticus (Nile Tilapia), in the Anambra River. The sample included twenty fishes, of which eighteen were collected from the three sampling locations (the fish ports of Anambra River), namely Otu-nsugbe, Otuocha, and Ikemivite) while two control samples were collected from a pond about 200 m away from the river. The levels of heavy metals were determined using Varian AA 240 atomic absorption spectrophotometer (AAS). The results showed that the concentrations of heavy metals (cadmium and arsenic) in the sampled fishes from Anambra River exceeded the joint World Health Organization and Food and Agriculture Organization (FAO/WHO) standard for fish and fish product consumption, while the concentration of chromium, mercury, and lead are within the permissible limit. The study also showed the distribution of the heavy metals in the fish organ varies among fish species. Heavy metals occur higher in Clarias garepinus than in Oreochromis niloticus, while tissue preference for heavy metal accumulation is in the order of gill > liver > muscle. It was recorded from this study that the heavy metal concentration in the fish from the pond is generally higher than the fish from the river for some metals. The high level of heavy metals in the sampled fish was attributed to heavy metals contamination of the river as a result of various anthropogenic activities such as mining, burning of fossil fuel and emission from the exhaust of boats/vehicles, overuse of fertilizers and pesticides, discharge of effluent, sewage, and hospital waste. This study concluded that long-term consumption of fish from the river may pose health risks to the consumers due to the possible bioaccumulation of heavy metals, especially cadmium and arsenic. It was recommended that continuous monitoring of heavy metal levels in the fish and water, public awareness, and appropriate legislative provisions should be put in place to ensure that harvested fish and fish products may be safe for human consumption.
{"title":"Heavy Metal Concentration in Fish Species Clarias gariepinus (Catfish) and Oreochromis niloticus (Nile Tilapia) from Anambra River, Nigeria","authors":"E. Ogbuene, A. M. Oroke, C. T. Eze, E. Etuk, O. G. Aloh, F. E. Achoru, J. C. Ogbuka, O. J. Okolo, A. V. Ozorme, C. J. Ibekwe, C. A. Eze, S. Akatakpo","doi":"10.46488/nept.2024.v23i02.052","DOIUrl":"https://doi.org/10.46488/nept.2024.v23i02.052","url":null,"abstract":"Studies have emphasized that the presence of heavy metals in freshwater fish represents a global public health issue. Nigeria, being a developing nation with less emphasis on the quality of seafood consumed by the residents, ranks this study very vital. The policy implication of this study is the advancement of a healthy population in contemporary Nigeria. Hence, this study assessed heavy metal concentration in two fish species, Clarias gariepinus (Catfish) and Oreochromis niloticus (Nile Tilapia), in the Anambra River. The sample included twenty fishes, of which eighteen were collected from the three sampling locations (the fish ports of Anambra River), namely Otu-nsugbe, Otuocha, and Ikemivite) while two control samples were collected from a pond about 200 m away from the river. The levels of heavy metals were determined using Varian AA 240 atomic absorption spectrophotometer (AAS). The results showed that the concentrations of heavy metals (cadmium and arsenic) in the sampled fishes from Anambra River exceeded the joint World Health Organization and Food and Agriculture Organization (FAO/WHO) standard for fish and fish product consumption, while the concentration of chromium, mercury, and lead are within the permissible limit. The study also showed the distribution of the heavy metals in the fish organ varies among fish species. Heavy metals occur higher in Clarias garepinus than in Oreochromis niloticus, while tissue preference for heavy metal accumulation is in the order of gill > liver > muscle. It was recorded from this study that the heavy metal concentration in the fish from the pond is generally higher than the fish from the river for some metals. The high level of heavy metals in the sampled fish was attributed to heavy metals contamination of the river as a result of various anthropogenic activities such as mining, burning of fossil fuel and emission from the exhaust of boats/vehicles, overuse of fertilizers and pesticides, discharge of effluent, sewage, and hospital waste. This study concluded that long-term consumption of fish from the river may pose health risks to the consumers due to the possible bioaccumulation of heavy metals, especially cadmium and arsenic. It was recommended that continuous monitoring of heavy metal levels in the fish and water, public awareness, and appropriate legislative provisions should be put in place to ensure that harvested fish and fish products may be safe for human consumption.","PeriodicalId":18783,"journal":{"name":"Nature Environment and Pollution Technology","volume":"12 6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141274517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：