NanoImpact最新文献_第7页

Nano-sized polystyrene plastics toxicity: Necroptosis pathway caused by autophagy blockade and lysosomal dysfunction 纳米聚苯乙烯塑料毒性：自噬阻断和溶酶体功能障碍引起的坏死坏死途径。

IF 4.7 3区环境科学与生态学 Q2 ENVIRONMENTAL SCIENCES

NanoImpact

Pub Date : 2025-01-01 DOI: 10.1016/j.impact.2024.100537

Haiyan Wu , Runqiu Cai , Chaoyu Zhou , Yifei Yang , Xinyuan Tian , Zhongling Zhao , Qianyu Bai , Xuejiao Qiu , Qingyi Song , Lei Zhang , Huihui Bao , Tianlong Liu

The persistent detection of nano-sized plastic particles in humans, animals, and animal-derived products underscores the potential impact of these particles on living organisms. Consequently, the toxicology of such particles has emerged as a pivotal research interests in recent years. In this study, NP was synthesized successfully with an average particle size of 100 nm using a emulsion polymerization method as model particles. Following co-incubation of IEC-6 cells with NP for 24–168 h, a notable inhibition of cell viability and proliferation was observed. The significant activation of autophagy and a concomitant blockage of autophagic flux in IEC-6 cells after 24–72 h of co-incubation with NP were unveiled by transmission electron microscopy, western blotting, and double-fluorescent autophagy analysis. A significant increase in the number of lysosomes and an increase in the expression of hydrolase CTSB were detected, indicating dysregulation of lysosomal function. The subsequent transcriptomic and metabolomics analyses, coupled with the observation of activated lysosomes and the RIPK1-RIPK3-MLKL/PYGL pathway, led us to posit that the blockade of autophagy and lysosomal dysfunction, culminating in lysosomal membrane permeabilization (LMP) induced necroptosis, constitutes one of the mechanisms contributing to the cytotoxicity of NP.

Synopsis

The cytotoxicity and its related mechanisms of nano-plastic is still unclear. This study found that nano-plastics may induce necroptosis in cells, and autophagy blockade and lysosomal dysfunction are prodromal manifestations.

在人类、动物和动物衍生产品中持续检测到纳米级塑料颗粒，强调了这些颗粒对生物体的潜在影响。因此，近年来，这些颗粒的毒理学已成为一个关键的研究兴趣。本研究以乳液聚合法为模型粒子成功合成了平均粒径为100 nm的NP。IEC-6细胞与NP共孵育24-168 h后，观察到细胞活力和增殖受到明显抑制。透射电镜、western blotting和双荧光自噬分析显示，与NP共孵育24-72 h后，IEC-6细胞的自噬明显激活，自噬通量阻滞。检测到溶酶体数量显著增加，水解酶CTSB表达增加，表明溶酶体功能失调。随后的转录组学和代谢组学分析，加上对活化溶酶体和RIPK1-RIPK3-MLKL/PYGL通路的观察，使我们假设自噬和溶酶体功能障碍的阻断，最终导致溶酶体膜透性（LMP）诱导的坏死坏死，是促成NP细胞毒性的机制之一。摘要：纳米塑料的细胞毒性及其相关机制尚不清楚。本研究发现纳米塑料可诱导细胞坏死，自噬阻断和溶酶体功能障碍是前驱症状。

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引用次数: 0

The risk of short-term microplastic exposure on female reproductive function: A rat model study 短期微塑料暴露对雌性生殖功能的影响：大鼠模型研究。

IF 4.7 3区环境科学与生态学 Q2 ENVIRONMENTAL SCIENCES

NanoImpact

Pub Date : 2025-01-01 DOI: 10.1016/j.impact.2025.100545

Zihan Wang , Ruiqing Zhang , Yuanzhen Zhang , Yao Xiong , Ming Zhang

Long-term effects of microplastics (MPs) exposure have been demonstrated to impair reproductive function. However, in real world, the exposure level of MP is not constant and it may vary in different individuals. This study aims to evaluate the impact of short-term exposure to MPs on ovarian and endometrial function in rat models. Serum steroid hormone concentrations and the expression of ovarian steroid hormone receptor were disturbed. We found that as MPs exposure concentration increased, thickness of the endometrial glandular epithelial layer and the number of endometrial glands decreased; the number of primordial follicles decreased, while the numbers of primary and secondary oocytes significantly increased, indicating a potential oocyte overactivation. Although short-term MP exposure appears to not influence embryo implantation and hormone functions, the results of this study highlight the potential of MPs to disrupt reproductive health in women.

长期接触微塑料（MPs）的影响已被证明会损害生殖功能。然而，在现实世界中，MP的暴露水平不是恒定的，在不同的个体中可能会有所不同。本研究旨在评估短期暴露于MPs对大鼠卵巢和子宫内膜功能的影响。血清类固醇激素浓度和卵巢类固醇激素受体的表达受到干扰。我们发现，随着MPs暴露浓度的增加，子宫内膜腺上皮层的厚度和子宫内膜腺的数量减少；原始卵泡数量减少，而初级和次级卵母细胞数量明显增加，表明可能存在卵母细胞过度激活。虽然短期接触多聚物似乎不会影响胚胎着床和激素功能，但这项研究的结果强调了多聚物破坏女性生殖健康的潜力。

引用次数: 0

Absolute quantitative lipidomics reveals the disturbance of lipid metabolism induced by oral exposure of titanium dioxide nanoparticles 绝对定量脂质组学揭示了口服二氧化钛纳米颗粒引起的脂质代谢紊乱。

IF 4.7 3区环境科学与生态学 Q2 ENVIRONMENTAL SCIENCES

NanoImpact

Pub Date : 2025-01-01 DOI: 10.1016/j.impact.2025.100554

Nairui Yu , Jiaqi Shi , Ying Ma , Yi Zhang , Li Guan , Zhangjian Chen , Guang Jia

The widespread use of titanium dioxide nanoparticles (TiO₂ NPs) as a white pigment in consumer goods increases the possibility of its release into the environment, which poses a great health risk to human beings. Many studies have proved the liver damage caused by TiO₂ NPs, but the research about the potential effects of TiO₂ NPs on liver lipid metabolism has been limited. Therefore, we selected Sprague-Dawley (SD) rats to explore the effects of long-term exposure to TiO₂ NPs on lipid metabolism. Rats were exposed to TiO₂ NPs at 0, 2, 10, 50 mg/kg body weight daily for 90 consecutive days. Subsequently, absolute quantitative lipidomics was used to ascertain variation of differential lipid metabolites in rat liver and serum. The results showed that TiO₂ NPs (50 mg/kg) changed 22 lipid metabolites such as DAG (18:2/20:5) and TAG (58:10/FA18:2) in rat liver. In the serum, the alteration of 119 lipid metabolites such as DAG (18:0/18:2) were more significant. There was a significant correlation between the different lipid metabolites in liver and serum. At the same time, it was observed that the relative expression levels of oxidative stress-related genes Nrf-2 and Ho-1 changed significantly, and they were closely related to differential metabolites. In conclusion, oral exposure of TiO₂ NPs has changed the lipid metabolomics of liver and serum, and the strong induction of oxidative stress may be related to it. TAG and DAG are key metabolites and metabolic pathways in two distinct biological samples, serving as potential indicators of liver injury to a certain extent.

二氧化钛纳米粒子（TiO2 NPs）作为白色颜料在消费品中的广泛使用，增加了其释放到环境中的可能性，对人类健康造成了极大的危害。许多研究证实了TiO2 NPs对肝脏的损害，但关于TiO2 NPs对肝脏脂质代谢潜在影响的研究有限。因此，我们选择SD （Sprague-Dawley）大鼠，研究长期暴露于TiO2 NPs对脂质代谢的影响。将大鼠每天以0、2、10、50 mg/kg体重的剂量暴露于TiO2 NPs中，连续90天。随后，采用绝对定量脂质组学方法确定大鼠肝脏和血清中差异脂质代谢物的变化。结果表明，TiO2 NPs（50 mg/kg）可改变大鼠肝脏中DAG（18:2/20:5）和TAG （58:10/FA18:2）等22种脂质代谢物。血清中DAG（18:0/18:2）等119种脂质代谢物变化更为显著。肝脏脂质代谢物与血清有显著相关性。同时，我们观察到氧化应激相关基因Nrf-2和Ho-1的相对表达水平发生了显著变化，它们与差异代谢物密切相关。综上所述，口服暴露TiO2 NPs改变了肝脏和血清脂质代谢组学，其对氧化应激的强烈诱导可能与此有关。TAG和DAG是两种不同生物样品中的关键代谢物和代谢途径，在一定程度上可以作为肝损伤的潜在指标。

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引用次数: 0

Gastrointestinal dysfunction as the main performance of the oral toxicity of titanium dioxide nanoparticle on gastric ulcer rats 胃肠功能紊乱是纳米二氧化钛颗粒对胃溃疡大鼠口服毒性的主要表现。

IF 4.7 3区环境科学与生态学 Q2 ENVIRONMENTAL SCIENCES

NanoImpact

Pub Date : 2025-01-01 DOI: 10.1016/j.impact.2025.100551

Yun Wang , Chen Li , Te Ba , Shengyuan Wang , Langzhi He , Zhangjian Chen , Ji Pu , Xiaoxing Cui , Guang Jia

Titanium dioxide nanoparticles (TiO₂ NPs) have promising applications in food additives and pharmaceutical dressings, raising concerns about their oral safety. The current studies mainly focus on healthy groups, and the effect of TiO₂ NPs on the patient population is rarely known. Here, a comprehensive toxicity study of TiO₂ NPs (75 ± 15 nm, anatase) in gastric ulcer rats (male 8-week old Sprague-Dawley rats) is reported following oral exposure at dose of 0, 10, 50, 200 mg/kg body weight per day for 30 days. The gastric ulcer rats were produced by submucosal injection of acetic acid solution into the rat stomach. The healthy rats were used as the normal control. We evaluated nanoparticle biodistribution, systemic toxicity, and gastrointestinal function indices in the rats. Our findings indicate that oral administration of TiO₂ NPs resulted in minimal intestinal absorption and transport with limited systemic organ toxicity. The internalization of TiO₂ NPs and activation of mast cells in the stomach tissues, along with the low serum levels of histamine and IgE, suggest a localized allergic reaction rather than a systemic one. Furthermore, the notably reduced plasma levels of D-lactate and the activity of diamine oxidase (DAO) indicated the upregulation of intestinal barrier function. These statistically significant results indicated that gastrointestinal dysfunction was the main performance of the oral toxicity of TiO₂ NPs on gastric ulcer rats, emphasizing the importance of controlling the intake of TiO₂ NPs in patients with gastric ulcers.

二氧化钛纳米粒子（TiO2 NPs）在食品添加剂和药物敷料中有很好的应用前景，但其口服安全性引起了人们的关注。目前的研究主要集中在健康人群，TiO2 NPs对患者群体的影响尚不清楚。本文报道了一项全面的胃溃疡大鼠（雄性8周龄Sprague-Dawley大鼠）口服0、10、50、200 mg/kg体重/天剂量的TiO2 NPs（75 ± 15 nm，锐钛矿）的毒性研究，持续30 天。采用胃粘膜下注射醋酸溶液制备大鼠胃溃疡模型。以健康大鼠为正常对照。我们评估了纳米颗粒在大鼠体内的生物分布、全身毒性和胃肠道功能指标。我们的研究结果表明，口服TiO2 NPs的肠道吸收和运输最小，全身器官毒性有限。胃组织中TiO2 NPs的内化和肥大细胞的激活，以及血清组胺和IgE的低水平，提示是局部过敏反应，而不是全身性过敏反应。血浆d -乳酸水平和二胺氧化酶（DAO）活性显著降低，表明肠道屏障功能上调。这些具有统计学意义的结果表明，胃肠道功能障碍是TiO2 NPs对胃溃疡大鼠口服毒性的主要表现，强调了胃溃疡患者控制TiO2 NPs摄入量的重要性。

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引用次数: 0

Mixtures of polystyrene micro and nanoplastics affects fat and glucose metabolism in 3T3-L1 adipocytes and zebrafish larvae 聚苯乙烯微塑料和纳米塑料的混合物影响3T3-L1脂肪细胞和斑马鱼幼虫的脂肪和葡萄糖代谢。

IF 4.7 3区环境科学与生态学 Q2 ENVIRONMENTAL SCIENCES

NanoImpact

Pub Date : 2025-01-01 DOI: 10.1016/j.impact.2025.100549

Jung Eun Kim , Narayan Sah Sonar , Laxmi Sen Thakuri , Jin Woo Park , Ki-Tae Kim , Dong Young Rhyu

Microplastics (MPs) and nanoplastics (NPs) are pervasive pollutants that pose a hazard to human health. Although most previous studies have investigated the effects of MPs and NPs on digestion, oxidative stress, and inflammation in diverse models, the combined effect of plastic mixtures (PM) containing MPs and NPs on obesity and type 2 diabetes mellitus (T2DM) remains unknown. The hypothesis of our study is to verify the association between PM exposure and clinical features of metabolic diseases such as lipogenesis and insulin resistance. Therefore, we investigated the effects of PM on fat and glucose metabolism in 3T3-L1 cells and high-fat diet (HFD)-induced zebrafish larvae. PM exposure increased cell viability, differentiation, adipogenesis (PPARγ and C/EBPα), and lipogenesis (FAS and SREBP-1c), while it decreased glucose uptake and inhibited insulin signal (IRS1, PI3K, AKT, and GLUT4) expression 3T3-L1 cells. In zebrafish larvae, PM mainly bioaccumulated in the intestine and pancreatic tissue, reducing glucose uptake and increasing body weight and blood glucose compared to controls. Moreover, PM significantly increased adipogenic differentiation (PPARγ) and synthesis (FASN and FABP), proinflammatory cytokines (TNF-α and IL-6), and gluconeogenesis (PCK1 and G6Pase). Conversely, energy and fat metabolism (AMPKα and adiponectin), insulin production (INSα), signaling pathway (IRS1, AKT, and GLUT2), and anti-inflammatory cytokines (IL-10 and IL-4) were suppressed. Overall, this study sheds light on the mechanisms responsible for the detrimental effects of PM exposure on fat and glucose metabolism, providing insights into metabolic disorders, like type 2 diabetes, in both in vitro and in vivo models.

微塑料（MPs）和纳米塑料（NPs）是普遍存在的污染物，对人类健康构成危害。尽管大多数先前的研究已经在不同的模型中研究了MPs和NPs对消化、氧化应激和炎症的影响，但含有MPs和NPs的塑料混合物（PM）对肥胖和2型糖尿病（T2DM）的综合影响尚不清楚。我们研究的假设是验证PM暴露与代谢性疾病（如脂肪生成、脂肪生成和胰岛素抵抗）的临床特征之间的关系。因此，我们研究了PM对3个 T3-L1细胞和高脂饲料（HFD）诱导的斑马鱼幼虫脂肪和葡萄糖代谢的影响。PM暴露增加细胞活力、分化、脂肪生成（PPARγ和C/EBPα）和脂肪生成（FAS和SREBP-1c），同时降低葡萄糖摄取并抑制胰岛素信号（IRS1、PI3K、AKT和GLUT4）表达3 T3-L1细胞。在斑马鱼幼虫中，PM主要在肠道和胰腺组织中生物积累，与对照组相比，减少葡萄糖摄取，增加体重和血糖。此外，PM显著增加了脂肪生成分化（PPARγ）和合成（FASN和FABP）、促炎细胞因子（TNF-α和IL-6）和糖异生（PCK1和G6Pase）。相反，能量和脂肪代谢（AMPKα和脂联素）、胰岛素产生（INSα）、信号通路（IRS1、AKT和GLUT2）和抗炎细胞因子（IL-10和IL-4）被抑制。总的来说，这项研究揭示了PM暴露对脂肪和葡萄糖代谢的有害影响的机制，在体外和体内模型中为代谢紊乱（如2型糖尿病）提供了见解。

{"title":"Mixtures of polystyrene micro and nanoplastics affects fat and glucose metabolism in 3T3-L1 adipocytes and zebrafish larvae","authors":"Jung Eun Kim , Narayan Sah Sonar , Laxmi Sen Thakuri , Jin Woo Park , Ki-Tae Kim , Dong Young Rhyu","doi":"10.1016/j.impact.2025.100549","DOIUrl":"10.1016/j.impact.2025.100549","url":null,"abstract":"<div><div>Microplastics (MPs) and nanoplastics (NPs) are pervasive pollutants that pose a hazard to human health. Although most previous studies have investigated the effects of MPs and NPs on digestion, oxidative stress, and inflammation in diverse models, the combined effect of plastic mixtures (PM) containing MPs and NPs on obesity and type 2 diabetes mellitus (T2DM) remains unknown. The hypothesis of our study is to verify the association between PM exposure and clinical features of metabolic diseases such as lipogenesis and insulin resistance. Therefore, we investigated the effects of PM on fat and glucose metabolism in 3T3-L1 cells and high-fat diet (HFD)-induced zebrafish larvae. PM exposure increased cell viability, differentiation, adipogenesis (<em>PPARγ</em> and <em>C/EBPα</em>), and lipogenesis (<em>FAS</em> and <em>SREBP-1c</em>), while it decreased glucose uptake and inhibited insulin signal (<em>IRS1</em>, <em>PI3K</em>, <em>AKT,</em> and <em>GLUT4</em>) expression 3T3-L1 cells. In zebrafish larvae, PM mainly bioaccumulated in the intestine and pancreatic tissue, reducing glucose uptake and increasing body weight and blood glucose compared to controls. Moreover, PM significantly increased adipogenic differentiation (<em>PPARγ</em>) and synthesis (<em>FASN</em> and <em>FABP</em>), proinflammatory cytokines (<em>TNF-α</em> and <em>IL-6</em>), and gluconeogenesis (<em>PCK1</em> and <em>G6Pase</em>). Conversely, energy and fat metabolism (<em>AMPKα</em> and <em>adiponectin</em>), insulin production (<em>INSα</em>), signaling pathway (<em>IRS1</em>, <em>AKT,</em> and <em>GLUT2</em>), and anti-inflammatory cytokines (<em>IL-10</em> and <em>IL-4</em>) were suppressed. Overall, this study sheds light on the mechanisms responsible for the detrimental effects of PM exposure on fat and glucose metabolism, providing insights into metabolic disorders, like type 2 diabetes, in both in vitro and in vivo models.</div></div>","PeriodicalId":18786,"journal":{"name":"NanoImpact","volume":"37 ","pages":"Article 100549"},"PeriodicalIF":4.7,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143449725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nano versus bulk: Evaluating the toxicity of lanthanum, yttrium, and cerium oxides on Enchytraeus crypticus 纳米与体积：评估镧、钇和铈氧化物对隐斑斑腹虫的毒性。

IF 4.7 3区环境科学与生态学 Q2 ENVIRONMENTAL SCIENCES

NanoImpact

Pub Date : 2025-01-01 DOI: 10.1016/j.impact.2024.100540

Joana Santos , Inês Neca , Ana Capitão , João Nogueira , José A.S. Santos , Edgar Pinto , Angela Barreto , Ana L. Daniel-da-Silva , Vera L. Maria

Considering the increase in demand for rare earth elements (REEs) and their accumulation in soil ecosystems, it is crucial to understand their toxicity. However, the impact of lanthanum, yttrium and cerium oxides (La₂O₃, Y₂O₃ and CeO₂, respectively) on soil organisms remains insufficiently studied. This study aims to unravel the effects of La₂O₃, Y₂O₃ and CeO₂ nanoparticles (NPs) and their corresponding bulk forms (0, 156, 313, 625, 1250 and 2500 mg/kg) on the terrestrial species Enchytraeus crypticus. The effects on survival, reproduction (21 days (d)), avoidance behavior (2 d) and DNA integrity (2 and 7 d) of E. crypticus were evaluated. No significant effects on survival were observed. For La₂O₃, the bulk form affected more endpoints than the NPs, inducing avoidance behavior (1250 mg/kg) and DNA damage (1250 mg/kg - 2 d; 2500 mg/kg - 7 d). The Y₂O₃ NPs demonstrated higher toxicity than the bulk form: decreased reproduction (≥ 1250 mg/kg); induced avoidance behavior (≥ 625 mg/kg) and DNA damage (≥ 156 mg/kg - 2 d; 2500 mg/kg - 7 d). For CeO₂, both forms exhibited similar toxicity, decreasing reproduction (625 mg/kg for bulk and 2500 mg/kg for NPs) and inducing DNA damage at all tested concentrations for both forms. REEs oxides toxicity was influenced by the REEs type and concentration, exposure time and material form, suggesting different modes of action. This study highlights the distinct responses of E. crypticus after exposure to REEs oxides and shows that REEs exposure may differently affect soil organisms, emphasizing the importance of risk assessment.

考虑到稀土元素需求的增加及其在土壤生态系统中的积累，了解其毒性至关重要。然而，镧、钇和铈氧化物（分别为La2O3、Y2O3和CeO2）对土壤生物的影响尚未得到充分研究。本研究旨在揭示La2O3、Y2O3和CeO2纳米颗粒（NPs）及其相应的体积形态（0、156、313、625、1250和2500 mg/kg）对陆生物种隐斑Enchytraeus crypticus的影响。对生存的影响,繁殖(21 天(d)),回避行为(2 d)和DNA完整性(2和7 d)的大肠crypticus进行评估。未观察到对生存率的显著影响。对于La2O3，体积形态比NPs影响更多的端点，诱导回避行为（1250 mg/kg）和DNA损伤（1250 mg/kg - 2 d）；2500 mg/kg - 7 d)。Y2O3 NPs表现出比散装形式更高的毒性：繁殖减少（≥1250 mg/kg）；诱发回避行为（≥625 mg/kg）和DNA损伤（≥156 mg/kg - 2 d）；对于CeO2，两种形式都表现出相似的毒性，在所有测试浓度下都能降低繁殖（散装625 mg/kg， NPs 2500 mg/kg），并诱导DNA损伤。稀土氧化物的毒性受稀土类型和浓度、暴露时间和物质形态的影响，表现出不同的作用模式。本研究强调了隐隐隐门线虫暴露于稀土氧化物后的不同反应，表明稀土暴露可能对土壤生物产生不同的影响，强调了风险评估的重要性。

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引用次数: 0

Metabolomics reveals the toxicity of polystyrene nanoplastics in the gills of Acrossocheilus yunnanensis 代谢组学揭示了聚苯乙烯纳米塑料在云南对虾鳃中的毒性

IF 4.7 3区环境科学与生态学 Q2 ENVIRONMENTAL SCIENCES

NanoImpact

Pub Date : 2025-01-01 DOI: 10.1016/j.impact.2025.100552

Qianqian Xiang , Ruisi Yang , Zexiang Hua , Peng Wang , Jilai Zhang , Wenyu Long , Xuexiu Chang

Although the ecotoxicity of polystyrene nanoplastics (PS-NP) to fish has been widely reported, their impact on the metabolic processes in fish gills and the underlying mechanisms remains unclear. Here, we investigated the effects of PS-NP on the morphology, oxidative stress, and metabolism of Acrossocheilus yunnanensis gills using conventional physicochemical indicators and metabolomics analysis. The results showed that PS-NP caused oxidative stress, and resulted in gill tissue lesions (e.g., proliferation and sloughing of gill epithelial cells). Metabolomics results showed that PS-NP exposure induced 75–164 differentially expressed metabolites (DEMs) in fish gills, and they were mainly related to lipid metabolism. DEMs induced by high concentration of PS-NP compared with low concentration of PS-NP were not only significantly enriched in glycerophospholipid metabolism, but also in sphingolipid metabolism, nucleic acid metabolism, and a variety of signaling pathways. In conclusion, the results of this work suggest that PS-NP cause disruption of phospholipid metabolism mainly by disrupting the integrity of gill tissue, which provides a new perspective for understanding the impact mechanism of PS-NP on fish gills. Given that fish play essential roles in maintaining ecological balance, the adverse effects of PS-NP on fish gills could ultimately disrupt the stability and health of the aquatic ecosystem.

尽管聚苯乙烯纳米塑料（PS-NP）对鱼类的生态毒性已被广泛报道，但其对鱼鳃代谢过程的影响及其潜在机制尚不清楚。本文采用常规理化指标和代谢组学分析方法，研究了PS-NP对云南对虾鳃形态、氧化应激和代谢的影响。结果表明，PS-NP引起氧化应激，导致鳃组织损伤（如鳃上皮细胞增殖和脱落）。代谢组学结果显示，PS-NP暴露诱导了75-164种鱼鳃差异表达代谢物（DEMs），这些代谢物主要与脂质代谢有关。与低浓度PS-NP相比，高浓度PS-NP诱导的dem不仅在甘油磷脂代谢方面显著富集，而且在鞘脂代谢、核酸代谢和多种信号通路方面也显著富集。综上所述，本研究结果表明，PS-NP主要通过破坏鳃组织的完整性来破坏磷脂代谢，这为理解PS-NP对鱼鳃的影响机制提供了新的视角。鉴于鱼类在维持生态平衡中起着至关重要的作用，PS-NP对鱼鳃的不良影响最终可能会破坏水生生态系统的稳定和健康。

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引用次数: 0

Bayesian network modelling for predicting the environmental hazard of silver nanomaterials in soils 土壤中银纳米材料环境危害的贝叶斯网络预测。

IF 4.7 3区环境科学与生态学 Q2 ENVIRONMENTAL SCIENCES

NanoImpact

Pub Date : 2025-01-01 DOI: 10.1016/j.impact.2025.100553

Irini Furxhi , Sarah Roberts , Richard Cross , Elise Morel , Anna Costa , Elma Lahive

In alignment with the European Union's Green Deal, which directs safe and sustainable practices for all chemicals, including nanomaterials (NMs) and advanced materials (AdMa), this study addresses the environmental hazard of silver NMs to terrestrial ecosystems. In the context of safe and sustainable by design (SSbD) framework, there is a need for methodologies that integrate pHysicochemical characteristics and experimental conditions to reliably predict their hazards to exposed species. Bayesian Networks (BN) represent a pivotal machine-learning (ML) tool with the potential to accelerate the SSbD process by leveraging predictive capabilities. In this study, we employed BN models trained on a literature-derived dataset capturing the ecotoxicity of silver (Ag) NMs in soils, focusing on predicting chronic no-observed effect concentrations (chronic NOECs). The model incorporates physicochemical characteristics such as surface treatment, nominal particle diameter and particle shape as provided by manufacturers, species information such as life stage and taxonomic class, and exposure medium characteristics. The BN, refined through expert insights, achieved an average predictive accuracy of approximately 82 % across the output labels. The study also extracted interpretable rules from the BN, outlining environmental safety criteria and identified key factors influencing NM hazard for terrestrial organisms. The critical need for experimental datasets that provide fuller details of physiochemical characteristics and experimental conditions, as well as current limitations, are highlighted. This modelling approach facilitates the rapid screening of the potential hazards of AgNMs to terrestrial ecosystems, with the potential to accelerate safety evaluations and rationalise experimental demands.

欧盟的 "绿色协议 "要求对包括纳米材料（NMs）和先进材料（AdMa）在内的所有化学品采取安全和可持续的做法，本研究旨在探讨银纳米材料对陆地生态系统的环境危害。在安全与可持续设计（SSbD）框架下，需要综合 pHysicochemical 特性和实验条件的方法，以可靠地预测其对暴露物种的危害。贝叶斯网络（BN）是一种重要的机器学习（ML）工具，具有利用预测能力加快 SSbD 进程的潜力。在本研究中，我们采用了贝叶斯网络（BN）模型，该模型是在文献数据集上训练而成的，捕捉了土壤中银（Ag）非金属的生态毒性，重点预测了慢性无观测效应浓度（慢性 NOECs）。该模型纳入了制造商提供的物理化学特征（如表面涂层、标称颗粒直径和颗粒形状）、物种信息（如生命阶段和分类类别）以及暴露介质特征。通过专家意见改进的 BN 在所有输出标签中实现了约 82% 的平均预测准确率。研究还从生物网络中提取了可解释的规则，概述了环境安全标准，并确定了影响陆生生物 NM 危害的关键因素。研究强调了对提供更全面的生化特征和实验条件细节的实验数据集的迫切需要，以及目前的局限性。这种建模方法有助于快速筛查 AgNMs 对陆地生态系统的潜在危害，从而有可能加快安全评估并使实验需求合理化。

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引用次数: 0

A catalytic perspective to nanomaterials reactivity-based toxicity; implications for single- and multiple-component nanomaterials (nanocomposites) 纳米材料反应毒性的催化研究对单组分和多组分纳米材料（纳米复合材料）的影响。

IF 4.7 3区环境科学与生态学 Q2 ENVIRONMENTAL SCIENCES

NanoImpact

Pub Date : 2025-01-01 DOI: 10.1016/j.impact.2025.100542

Miguel A. Bañares, Victor Alcolea-Rodriguez , Raquel Portela

The extended use of a given product normally precedes concerns about it. The reactivity-based nanotoxicity is a major concern that must be tackled from its fundamental understanding to its regulatory management. Moreover, concepts and ideas must seamlessly flow between relevant performers. Functional nanomaterials have been used in many fields; among these, catalysis is probably the earliest more extended application of nanomaterials, these are engineered to afford specific properties, and are typically known as Engineered Nanomaterials (ENMs). Heterogenous catalysis shares its basic features with reactivity-based toxicity. In both cases, we are dealing with phenomena triggered by reactions occurring at the surface of the nanomaterial. Therefore, the extensive knowledge in heterogeneous catalysis is key to understanding reactivity-based nanotoxicology. In this regard, determining surface exposure is fundamental to mechanistically comprehend dose-response, similar to how catalysis shifted from mass-based to surface-centered metrics. Catalysis science made a further refinement iteration: reactions occur at surfaces, though not all surfaces are necessarily reactive, making it crucial to normalize per reactive site. This perspective focuses on two key aspects that link heterogeneous catalysis and reactivity-based nanotoxicity: the reactive sites on the surface of nanomaterials and how different combinations of nanomaterials appear and perform. A comment is also made regarding the somewhat vague term ‘multicomponent nanomaterial,’ which is contrasted with the well-defined, established, and widely accepted term ‘nanocomposite’ within the chemical community. Clear and precise terminology and concepts are essential for effective research and regulation.

一种特定产品的长期使用通常先于对其的担忧。基于反应性的纳米毒性是一个必须从其基本理解到其监管管理的主要问题。此外，概念和想法必须在相关执行者之间无缝流动。功能纳米材料在许多领域得到了应用；其中，催化可能是纳米材料最早的更广泛的应用，这些材料被设计为提供特定的性能，通常被称为工程纳米材料（enm）。多相催化与基于反应性的毒性具有相同的基本特征。在这两种情况下，我们都在处理由纳米材料表面发生的反应引发的现象。因此，多相催化的广泛知识是理解基于反应性的纳米毒理学的关键。在这方面，确定表面暴露是机械理解剂量反应的基础，类似于催化如何从基于质量的指标转变为以表面为中心的指标。催化科学做了进一步的改进迭代：反应发生在表面上，尽管不是所有的表面都必须是反应性的，这使得标准化每个反应位点变得至关重要。这一观点集中在连接多相催化和基于反应性的纳米毒性的两个关键方面：纳米材料表面的反应位点以及纳米材料的不同组合如何出现和表现。本文还对“多组分纳米材料”这一模糊的术语进行了评论，这与化学学界定义明确、已建立并被广泛接受的术语“纳米复合材料”形成了对比。清晰和精确的术语和概念对于有效的研究和监管至关重要。

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引用次数: 0

Regulatory preparedness for multicomponent nanomaterials: Current state, gaps and challenges of REACH 多组分纳米材料的监管准备：REACH的现状、差距和挑战。

IF 4.7 3区环境科学与生态学 Q2 ENVIRONMENTAL SCIENCES

NanoImpact

Pub Date : 2025-01-01 DOI: 10.1016/j.impact.2024.100538

Neil Hunt , Vikram Kestens , Kirsten Rasmussen , Elena Badetti , Lya G. Soeteman-Hernández , Agnes G. Oomen , Willie Peijnenburg , Danail Hristozov , Hubert Rauscher

In 2018 the European Commission adopted revisions to the Annexes of Regulation (EC) No 1907/2006 concerning registration, evaluation, authorisation and restriction of chemicals (REACH) to introduce nanomaterial-specific clarifications and provisions. Multicomponent nanomaterial (MCNM) is a non-regulatory term that has been used in recent EU-funded projects to describe nanomaterials with a complex structure and/or composition and which are expected to be increasingly used in products in the near future. This paper examines the regulatory preparedness of REACH, and its revised Annexes, for MCNMs. Several situations have been identified where there is potential confusion and uncertainty around how regulatory definitions used in REACH should be applied to MCNMs. If a MCNM cannot be identified as falling within a specific definition, understanding the regulatory obligations that apply to it is very difficult. Examples of these grey areas include how the term “surface functionalisation or modification” applies when a chemical is physisorbed to the surface of a nanoform, and the identity of the substance that should be registered when the modification takes it outside the definition of a nanoform. We conclude that the regulatory preparedness can be improved by amending the REACH guidance on information requirements for nanoforms and revising the definition of “nanoform” in line with the updated EC Recommendation on the definition of nanomaterial.

2018年，欧盟委员会通过了关于化学品注册、评估、授权和限制（REACH）的法规（EC） No 1907/2006附件的修订，以引入纳米材料特定的澄清和规定。多组分纳米材料（MCNM）是一个非监管术语，在最近欧盟资助的项目中用于描述具有复杂结构和/或成分的纳米材料，预计在不久的将来将越来越多地用于产品中。本文探讨了针对MCNMs的REACH及其修订附件的监管准备。已经确定了几种情况，其中关于如何将REACH中使用的监管定义应用于MCNMs存在潜在的混淆和不确定性。如果MCNM不能被确定为属于特定定义，那么理解适用于它的监管义务是非常困难的。这些灰色地带的例子包括当化学物质被物理吸附到纳米形态的表面时，术语“表面功能化或修饰”如何适用，以及当修饰使其超出纳米形态的定义时，应该注册的物质的身份。我们的结论是，可以通过修改REACH关于纳米形式信息要求的指南，并根据更新的EC关于纳米材料定义的建议修改“纳米形式”的定义来改进监管准备。

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