NanoImpact最新文献_第6页

Dispersion protocols have minimal impact on the biomolecular corona of advanced nanomaterials in cell culture assays 在细胞培养实验中，分散方案对先进纳米材料的生物分子电晕影响最小

IF 4.7 3区环境科学与生态学 Q2 ENVIRONMENTAL SCIENCES

NanoImpact

Pub Date : 2025-04-01 DOI: 10.1016/j.impact.2025.100560

Alberto Martinez-Serra , Asia Saorin , Ana Serrano-Lotina , Ahmed Subrati , Mahmoud G. Soliman , Danail Hristozov , Miguel A. Bañares , Philip Demokritou , Marco P. Monopoli

Industrial sectors have largely invested in the use of advanced nanomaterials (NMs), which are currently being implemented in a wide range of applications. However, the potential exposure to living beings and the environment still remains a concern. While some of these materials were not designed to be dispersible in aqueous media, the development of dispersion protocols to ensure compatibility with the in vitro and in vivo assays has become crucial for the correct assessment of the studies. NMs' identity in biological media is significantly influenced by the formation of a biomolecular corona on its surface. However, this corona might be affected by the dispersion method, altering their physicochemical characteristics and complicating the understanding of their interactions with biological systems. Therefore, understanding the efficiency of dispersion protocols and their influence on the biological identity of NMs is fundamental. However, systematic studies on the effects of dispersion protocols are still lacking, making this a crucial yet overlooked aspect in the field. This study aims to compare two standard dispersion protocols, commonly known as Harvard and Nanogenotox, and evaluate their impact on the biomolecular corona formation across a selection of advanced industrial NMs. To this aim, different techniques were used to assess particle size, colloidal stability and ion release, as well as protein and sialic acid content and abundance in the corona. Results show that the dispersion protocol modestly alters nanoparticle size and agglomeration state, and proteomics analysis revealed that each nanoparticle type forms a distinct corona, influenced by the distinct surface modifications. The presence of bovine serum albumin (BSA) in the Nanogenotox protocol minimally affected the overall trends in protein composition between the two protocols. These findings emphasize the significance of the dispersion protocol in nanotoxicology assays and demonstrate that variations between these methods do not play a decisive role in shaping the bio-identity and potential biological effects of advanced and multicomponent NMs.

工业部门已经大量投资于先进纳米材料（NMs）的使用，目前正在广泛应用中实施。然而，对生物和环境的潜在暴露仍然是一个问题。虽然其中一些材料的设计不适合在水介质中分散，但开发分散方案以确保与体外和体内试验的兼容性对于正确评估研究至关重要。生物介质表面形成的生物分子电晕会显著影响纳米粒子在生物介质中的身份。然而，这种电晕可能受到分散方法的影响，改变了它们的物理化学特性，并使对它们与生物系统相互作用的理解复杂化。因此，了解分散方案的效率及其对NMs生物特性的影响是至关重要的。然而，关于分散协议影响的系统研究仍然缺乏，使这成为该领域一个至关重要但被忽视的方面。本研究旨在比较两种标准分散方案（通常称为Harvard和Nanogenotox），并评估它们对先进工业NMs中生物分子电晕形成的影响。为此，研究人员使用了不同的技术来评估日冕中的颗粒大小、胶体稳定性和离子释放，以及蛋白质和唾液酸的含量和丰度。结果表明，分散方案适度地改变了纳米颗粒的大小和团聚状态，蛋白质组学分析表明，每种纳米颗粒类型形成不同的电晕，受到不同表面修饰的影响。纳米基因毒素方案中牛血清白蛋白（BSA）的存在对两种方案之间蛋白质组成的总体趋势影响最小。这些发现强调了分散方案在纳米毒理学分析中的重要性，并表明这些方法之间的差异在形成先进和多组分纳米颗粒的生物特性和潜在生物效应方面并不起决定性作用。

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引用次数: 0

Death-driven exposure: Necrotrophic transfer of polylactic acid and polystyrene microplastics alters biochemical architecture in the saprophagous insect Chrysomya megacephala 死亡驱动的暴露：聚乳酸和聚苯乙烯微塑料的坏死性转移改变了腐生昆虫大头金蝇的生化结构

IF 4.7 3区环境科学与生态学 Q2 ENVIRONMENTAL SCIENCES

NanoImpact

Pub Date : 2025-04-01 DOI: 10.1016/j.impact.2025.100570

Ariane Guimarães , Raíssa Ferreira de Oliveira , Abraão Tiago Batista Guimarães , Rafaela Ribeiro de Brito , Aline Sueli de Lima Rodrigues , Letícia Paiva de Matos , Thiarlen Marinho da Luz , Guilherme Malafaia

Microplastic exposure (MPs) represents an emerging threat to terrestrial ecotoxicology, particularly within detritivorous ecosystems. In this study, we investigated transstadial bioaccumulation and the biochemical effects of necrotrophic exposure to polystyrene (PS-MPs) and polylactic acid microplastics (PLA-MPs) in Chrysomya megacephala. Mice contaminated with varying concentrations of the polymers were used as larval decomposition substrates. To confirm the prior internalization of MPs, third-instar larvae (L3) were analyzed, revealing significant particle accumulation. We assessed MP retention and biochemical alterations in key physiological systems following adult emergence. The presence of MPs in newly emerged adults, at significantly lower concentrations than those observed in larvae, confirmed transstadial bioaccumulation and indicated partial retention during metamorphosis. Bioaccumulation was more pronounced at higher exposure concentrations. PS-MPs induced functional disorganization, characterized by increased reactive oxygen species (ROS), lipid peroxidation, proteolytic activity, and neuroendocrine dysfunctions. In contrast, PLA-MPs elicited more coordinated biochemical responses, with emphasis on antioxidant activation. Correlation network and functional trade-off analyses revealed distinct patterns of physiological reorganization between treatments. At the same time, trend tests and causal inference confirmed concentration-dependent effects and a direct association between exposure and biochemical dysfunction. The data suggest that necrotrophic exposure constitutes a functional pathway for MP redistribution in terrestrial environments, exerting systemic effects even in the absence of direct adult contact.

微塑料暴露（MPs）是对陆地生态毒理学的新威胁，特别是在营养性生态系统中。在这项研究中，我们研究了坏死性暴露于聚苯乙烯（PS-MPs）和聚乳酸微塑料（PLA-MPs）在大头金体内的生物积累和生化效应。被不同浓度的聚合物污染的小鼠作为幼虫分解的底物。为了证实MPs的优先内化，对三龄幼虫（L3）进行了分析，发现明显的颗粒积累。我们评估了成虫羽化后关键生理系统的MP保留和生化变化。新出现的成虫体内MPs的浓度明显低于幼虫体内的浓度，证实了跨体表生物积累，并表明在变态过程中存在部分滞留。在较高的暴露浓度下，生物积累更为明显。PS-MPs诱导功能紊乱，其特征是活性氧（ROS）、脂质过氧化、蛋白水解活性增加和神经内分泌功能障碍。相反，PLA-MPs引发了更协调的生化反应，重点是抗氧化激活。相关网络和功能权衡分析揭示了不同处理之间不同的生理重组模式。同时，趋势测试和因果推理证实了浓度依赖效应和暴露与生化功能障碍之间的直接联系。数据表明，坏死性暴露构成了陆地环境中MP再分配的功能途径，即使在没有直接成人接触的情况下也会产生全身影响。

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引用次数: 0

Microplastics: Hidden drivers of antimicrobial resistance in aquatic systems 微塑料：水生系统中抗菌素耐药性的隐藏驱动因素

IF 4.7 3区环境科学与生态学 Q2 ENVIRONMENTAL SCIENCES

NanoImpact

Pub Date : 2025-04-01 DOI: 10.1016/j.impact.2025.100566

Prasun Goswami , Kazuki Kanda , Yukino Tamamura-Andoh , Mafumi Watanabe , Keerthi S. Guruge

Microplastics (MPs) in aquatic ecosystems readily promote biofilm formation, creating the plastisphere, a dynamic interface that interacts with environmental pollutants and acts as a reservoir for microorganisms. Recent studies emphasize the plastisphere's contribution to the spread of pathogens, antibiotic-resistant genes (ARGs), and antimicrobial resistance (AMR) within aquatic organisms and across diverse environments, a phenomenon collectively called the ‘Plastiome’. Although the prevalence and effects of the plastisphere have been studied extensively, a systematic synthesis of updated insights into the behavior of the plastiome is urgently needed. This review explores the development and behavior of plastics, focusing on its interactions with ARGs and pathogens within aquatic ecosystems. Microplastics selectively enrich ARGs and pathogenic microorganisms, fostering unique microbial communities distinct from those in surrounding waters. The plastiome facilitates horizontal ARG propagation, increasing the quantity of antibiotic-resistant pathogens and presenting substantial risks to the hydrosphere and public health. Additionally, key research opportunities are identified and strategies are recommended to advance our understanding of plastiome-driven antibiotic resistance in aquatic environments.

水生生态系统中的微塑料（MPs）很容易促进生物膜的形成，形成塑性球，这是一个与环境污染物相互作用的动态界面，也是微生物的储存库。最近的研究强调了塑料圈对水生生物内部和不同环境中病原体、抗生素耐药基因（ARGs）和抗菌素耐药性（AMR）传播的贡献，这种现象统称为“Plastiome”。虽然塑料球的流行和影响已经得到了广泛的研究，但迫切需要系统地综合最新的见解，以了解质体的行为。本文综述了塑料的发展和行为，重点介绍了塑料与水生生态系统中ARGs和病原体的相互作用。微塑料选择性地丰富了ARGs和致病微生物，培养了与周围水域不同的独特微生物群落。质体促进了ARG的水平传播，增加了耐抗生素病原体的数量，并对水圈和公共卫生构成重大风险。此外，还确定了关键的研究机会并建议了策略，以促进我们对水生环境中plastiome驱动的抗生素耐药性的理解。

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引用次数: 0

Development and characterization of reference environmentally relevant micro-nano-plastics for risk assessment studies 用于风险评估研究的参考环境相关微纳米塑料的开发和特性

IF 4.7 3区环境科学与生态学 Q2 ENVIRONMENTAL SCIENCES

NanoImpact

Pub Date : 2025-04-01 DOI: 10.1016/j.impact.2025.100567

Milton Das , Leonardo Calderon , Dilpreet Singh , Satwik Majumder , Lila Bazina , Nachiket Vaze , Una Trivanovic , Glen DeLoid , Nubia Zuverza-Mena , Mandeep Kaur , Jakub Konkol , Nassifatou Koko Tittikpina , George Tsilomelekis , Omowunmi Sadik , Jason C. White , Philip Demokritou

Micro- and nano-plastics (MNPs) have become ubiquitous environmental pollutants. Extensive toxicological studies of MNPs have been conducted in recent years. However, because of the difficulties involved in extraction and collection of MNPs from environmental media, most of these studies have employed simplistic, pristine, spherical, micro- or nano-sized commercial MNPs, whose properties, including morphology, surface chemistry, and size, do not adequately approximate those of environmentally relevant MNPs. Here, we describe the development and use of methods for the synthesis of well characterized, environmentally relevant MNPs across the life cycle of a plastic material, in a property-controlled manner. Multiple degradation scenarios, including mechanical fragmentation (cryogenic milling), UV weathering, and thermal disintegration (incineration) were applied to virgin plastic materials (polyvinyl chloride, PVC; polyethylene terephthalate, PET; High density polyethylene, HDPE; Acrylonitrile butadiene styrene, ABS; polycarbonate, PC; and polypropylene, PP) to simulate the life cycle pathways that likely occur in the environment over time. The MNPs generated from these degradation processes were size fractionated using both “dry” and “wet” separation methods. Detailed physicochemical characterization of the size fractionated reference MNPs was performed to determine size, morphology, chemical and elemental compositions, and hydrophobicity. Microbiological sterility and endotoxin content of reference MNPs were also assessed. Protocols for storage of reference MNPs in controlled oxygen and moisture conditions for future use in toxicological studies are also described. The methodology developed in this study can be used to synthesize environmentally relevant reference MNPs across the life cycle of plastic materials for use in risk assessment studies.

微纳米塑料已成为普遍存在的环境污染物。近年来对MNPs进行了广泛的毒理学研究。然而，由于从环境介质中提取和收集MNPs的困难，这些研究大多采用了简单的、原始的、球形的、微或纳米尺寸的商业MNPs，其性质，包括形态、表面化学和尺寸，不能充分接近与环境相关的MNPs。在这里，我们以一种属性控制的方式描述了在塑料材料的整个生命周期中合成具有良好特征的、与环境相关的MNPs的方法的开发和使用。多种降解方案，包括机械破碎（深冷碾磨），紫外线风化和热解体（焚烧）应用于原始塑料材料(聚氯乙烯，PVC；聚对苯二甲酸乙二醇酯；PET；高密度聚乙烯；丙烯腈-丁二烯-苯乙烯；聚碳酸酯、电脑;和聚丙烯（PP）来模拟随着时间的推移，环境中可能发生的生命周期路径。由这些降解过程产生的MNPs使用“干”和“湿”分离方法进行粒度分馏。对粒径分级参考MNPs进行了详细的物理化学表征，以确定粒径、形态、化学和元素组成以及疏水性。对参考MNPs的微生物无菌性和内毒素含量也进行了评估。还描述了在控制氧气和湿度条件下储存参考MNPs的方案，以便将来在毒理学研究中使用。本研究开发的方法可用于在塑料材料的整个生命周期中合成与环境相关的参考MNPs，以用于风险评估研究。

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引用次数: 0

Scaling up the graphene production from R&D to the pilot plant stage: Implications for workers' exposure to airborne nano-objects 将石墨烯生产从研发阶段扩大到中试工厂阶段：对工人接触空气中纳米物体的影响。

IF 4.7 3区环境科学与生态学 Q2 ENVIRONMENTAL SCIENCES

NanoImpact

Pub Date : 2025-03-19 DOI: 10.1016/j.impact.2025.100555

Claudio Natale , Francesca Tombolini , Riccardo Ferrante , Francesca Sebastiani , Andrea Gordiani , Maurizio Manigrasso , Antonio Esau Del Rio Castillo , Francesco Bonaccorso , Stefania Sabella , Fabio Boccuni

Given the exceptional thermal, electrical, and mechanical properties of graphene, the interest is now shifting from scientific and technological application to industrial deployment, testified by the significant increase in demand for graphene-based products. Consequently, it is paramount that occupational safety and health (OSH) research now places utmost importance on ensuring the well-being of workers at every stage of graphene production. The present study evaluates workers' exposure potential during the production cycle of few-layer graphene (FLG) by liquid-phase exfoliation, incorporating the Prevention-through-Design approach in the transition from the laboratory scale to the pilot plant production. A measurement campaign was conducted according to the multi-metric approach proposed by the Organization for Economic Cooperation and Development and European Committee for Standardization guidelines. Multi-metric real-time instruments were used to determine particle number concentration (PNC), particle size distribution and lung deposited surface area (LDSA) along with time-integrated instrumentation to collect airborne ultrafine dust for off-line gravimetric analysis and chemical and morphological characterization. The obtained data indicate that the FLG powders storage, including the cleaning of equipment and surfaces, is the most critical step for exposed workers, with higher levels of PNC and LDSA compared to the other production phases. Recommendations for OSH risk mitigation strategies in the scaling up of the FLG production process have been proposed according to OSH principles for nano and advanced materials development. In particular, production and storage of FLG in liquid suspension or bound to a solid matrix should be preferred rather than in powder form. When not possible, a closed system with local exhaust ventilation is recommended. Finally, if the particles transport towards other areas of the plant is not properly mitigated, the sole use of personal protective equipment during the powder handling phases will be not sufficient for protecting workers from the potential exposure.

鉴于石墨烯优异的热学、电学和机械性能，人们的兴趣正从科学和技术应用转向工业应用，对石墨烯基产品需求的显著增长证明了这一点。因此，在石墨烯生产的每个阶段，职业安全与健康（OSH）研究都非常重视确保工人的福祉，这一点至关重要。本研究评估了工人在液相剥离制备少层石墨烯（FLG）的生产周期中的暴露潜力，并在从实验室规模到中试工厂生产的过渡中采用了预防-设计方法。根据经济合作与发展组织和欧洲标准化委员会的指导方针提出的多度量方法，开展了一项测量活动。采用多指标实时仪器测定空气中超细粉尘的颗粒数浓度（PNC）、粒径分布和肺沉积表面积（LDSA），并结合时间集成仪器采集空气中超细粉尘进行离线重量分析、化学和形态表征。所获得的数据表明，FLG粉末的储存，包括设备和表面的清洁，是暴露工人最关键的步骤，与其他生产阶段相比，PNC和LDSA的水平更高。根据纳米和先进材料开发的职业安全与健康原则，提出了在扩大FLG生产过程中减轻职业安全与健康风险战略的建议。特别是，FLG以液体悬浮液或与固体基质结合的方式生产和储存应优于以粉末形式。如果不可能，建议采用局部排气通风的封闭系统。最后，如果颗粒向工厂其他区域的转移没有得到适当的缓解，那么在粉末处理阶段单独使用个人防护设备将不足以保护工人免受潜在的暴露。

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引用次数: 0

The dispersion method does not affect the in vitro genotoxicity of multi-walled carbon nanotubes despite inducing surface alterations 分散方法不影响多壁碳纳米管的体外遗传毒性，尽管引起表面改变。

IF 4.7 3区环境科学与生态学 Q2 ENVIRONMENTAL SCIENCES

NanoImpact

Pub Date : 2025-01-01 DOI: 10.1016/j.impact.2024.100539

Michael J. Burgum , Víctor Alcolea-Rodríguez , Hanna Saarelainen , Raquel Portela , Julián J. Reinosa , José F. Fernández , Verónica I. Dumit , Julia Catalán , Felice C. Simeone , Lara Faccani , Martin J.D. Clift , Stephen J. Evans , Miguel A. Bañares , Shareen H. Doak

Multi-walled carbon nanotubes (MWCNTs) are a desirable class of high aspect ratio nanomaterials (HARNs) owing to their extensive applications. Given their demand, the growing occupational and consumer exposure to these materials has warranted an extensive investigation into potential hazards they may pose towards human health. This study utilised both the in vitro mammalian cell gene mutation and the cytokinesis-blocked micronucleus (CBMN) assays to investigate genotoxicity in human lymphoblastoid (TK6) and 16HBE14o⁻ human lung epithelial cells, following exposure to NM-400 and NM-401 MWCNTs for 24 h. To evaluate the potential for secondary genotoxicity, the CBMN assay was applied on a co-culture of 16HBE14o⁻ with differentiated human monocytic (dTHP-1) cells. In addition, two dispersion methods (NanoGenoTox vs. high shear mixing) were utilised prior to exposures and in acellular experiments to assess the effects on MWCNT oxidative potential, aspect ratio and surface properties. These were characterized in chemico as well as by electron microscopy and Raman spectroscopy. Structural damage of NM-400 was observed following both dispersion approaches; Raman spectra highlighted greater oxidative transformation under probe sonication as opposed to high shear mixing. Despite the changes to the oxidative potential of the MWCNTs, no statistically significant genotoxicity was observed under the conditions applied. There was also no visible signs of cellular internaliation of NM-400 or NM-401 into either cell type under the test conditions, which may support the negative genotoxic response. Whilst these HARNs may have oxidative potential, cells have natural protective mechanisms for repairing transient DNA damage. Therefore, it is crucial to evaluate biological endpoints which measure fixed DNA damage to account for the impact of DNA repair mechanisms.

多壁碳纳米管（MWCNTs）由于其广泛的应用而成为一类理想的高纵横比纳米材料（HARNs）。鉴于这些材料的需求，越来越多的职业和消费者接触到这些材料，有必要对它们可能对人类健康造成的潜在危害进行广泛调查。本研究利用体外哺乳动物细胞基因突变和细胞动力学阻断微核（CBMN）试验，研究暴露于NM-400和NM-401 MWCNTs 24 h后，人淋巴母细胞样细胞（TK6）和16HBE14o-人肺上皮细胞的遗传毒性。为了评估继发性遗传毒性的可能性，将CBMN试验应用于16HBE14o-与分化的人单核细胞（dTHP-1）共培养。此外，在暴露前和脱细胞实验中，使用了两种分散方法（NanoGenoTox和高剪切混合）来评估对MWCNT氧化电位、纵横比和表面性质的影响。用化学、电子显微镜和拉曼光谱对它们进行了表征。两种分散方法均观察到NM-400的结构损伤；拉曼光谱强调了探针超声下更大的氧化转变，而不是高剪切混合。尽管MWCNTs的氧化电位发生了变化，但在施加的条件下没有观察到统计学上显著的遗传毒性。在测试条件下，NM-400或NM-401也没有明显的细胞内化迹象，这可能支持负遗传毒性反应。虽然这些harn可能具有氧化潜能，但细胞具有修复瞬时DNA损伤的天然保护机制。因此，评估测量固定DNA损伤的生物终点以解释DNA修复机制的影响是至关重要的。

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引用次数: 0

Nanoplastics in focus: Exploring interdisciplinary approaches and future directions 纳米塑料聚焦：探索跨学科方法和未来方向。

IF 4.7 3区环境科学与生态学 Q2 ENVIRONMENTAL SCIENCES

NanoImpact

Pub Date : 2025-01-01 DOI: 10.1016/j.impact.2025.100544

Julien Gigault , Mélanie Davranche

Nanoplastics (NPs) are gaining increasing attention due to their widespread distribution and potential environmental and biological impacts. Spanning a variety of ecosystems – from soils and rivers to oceans and polar ice – NPs interact with complex biological and geochemical processes, posing risks to organisms across multiple trophic levels. Despite their growing presence, understanding the behavior, transport, and toxicity of nanoplastics remains challenging due to their diverse physical and chemical properties as well as the heterogeneity of environmental matrices. Currently, nanoplastics are often studied alongside microplastics as a single, homogeneous group, which obscures the nuanced behavior of NPs, particularly in terms of their colloidal properties and interactions within ecosystems. This perspective aims to highlight the critical gaps in nanoplastics research, stressing the importance for field studies and advanced detection/quantification methods to better capture their behavior across environmental interfaces. We advocate for a more integrated approach that account for the dynamic interactions between nanoplastics and surrounding biological, chemical, and physical environments, especially across key ecological gradients. Furthermore, long-term and transgenerational studies are essential to assess the chronic impacts of low-concentration nanoplastics exposure. Innovative and appropriate methodologies are needed to explore NP fate, transport, and toxicity in realistic environmental conditions. By combining advanced experimental tools, field studies, and ecological modeling frameworks, this paper outlines provides a roadmap for advancing our understanding of nanoplastics and their broader ecological impacts, ultimately shaping more effective environmental monitoring and mitigation strategies.

纳米塑料由于其广泛的分布和潜在的环境和生物影响而受到越来越多的关注。NPs跨越多种生态系统——从土壤和河流到海洋和极地冰——与复杂的生物和地球化学过程相互作用，对多个营养水平的生物构成风险。尽管纳米塑料的存在越来越多，但由于其不同的物理和化学性质以及环境基质的异质性，了解纳米塑料的行为、运输和毒性仍然具有挑战性。目前，纳米塑料经常与微塑料一起作为一个单一的、同质的群体进行研究，这掩盖了纳米塑料的细微行为，特别是在它们的胶体性质和生态系统内的相互作用方面。这一观点旨在强调纳米塑料研究中的关键空白，强调实地研究和先进的检测/量化方法的重要性，以更好地捕捉它们在环境界面上的行为。我们提倡采用一种更综合的方法来解释纳米塑料与周围生物、化学和物理环境之间的动态相互作用，特别是在关键的生态梯度上。此外，长期和跨代研究对于评估低浓度纳米塑料暴露的慢性影响至关重要。需要创新和适当的方法来探索NP在现实环境条件下的命运、运输和毒性。通过结合先进的实验工具、实地研究和生态建模框架，本文概述了一个路线图，以促进我们对纳米塑料及其更广泛的生态影响的理解，最终形成更有效的环境监测和缓解策略。

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引用次数: 0

Titanium dioxide nanoparticles - physicochemical characterization and cytotoxic risk 二氧化钛纳米颗粒。物理化学特性和细胞毒性风险。

IF 4.7 3区环境科学与生态学 Q2 ENVIRONMENTAL SCIENCES

NanoImpact

Pub Date : 2025-01-01 DOI: 10.1016/j.impact.2025.100543

Filip Kunc , Xiaomei Du , Andre Zborowski , Linda J. Johnston , David C. Kennedy

Titanium dioxide (TiO₂) nanoparticles (NPs) are incorporated into numerous consumer products yet data as to potential adverse health effects remains inconclusive. In this paper we physically characterize 16 nanoforms of TiO₂ from different manufacturers of different size, crystalline structure and surface chemistry. Physical measurements of the particles were performed and compared with those provided by manufacturers revealing several discrepancies. We then examined the biological effects of these particles in cell culture in 3 commonly used cell lines for testing materials. We were unable to validate that anatase particles are more cytotoxic than rutile particles as has been reported, and generally found that the particles produced few effects and no significant production of reactive oxygen species under the conditions used. While some particles do exhibit a dose dependent cytotoxicity that increases over time in some cell lines, the effects were not consistent between cell lines and do not appear to be linked to crystalline structure or any of the specific physical characteristics that were measured including, size, charge and surface composition, nor a correlation with the production of reactive oxygen species.

二氧化钛（TiO2）纳米颗粒（NPs）被纳入许多消费品中，但关于其潜在不利健康影响的数据仍不确定。本文对不同厂家生产的16种不同尺寸、晶体结构和表面化学性质的纳米TiO2进行了物理表征。对颗粒进行了物理测量，并与制造商提供的数据进行了比较，发现了一些差异。然后，我们在3种常用的细胞系中检测了这些颗粒在细胞培养中的生物学效应。我们无法证实锐钛矿颗粒比金红石颗粒具有更大的细胞毒性，并且通常发现在所使用的条件下，锐钛矿颗粒产生的效果很少，并且没有显著的活性氧产生。虽然某些颗粒确实表现出剂量依赖性的细胞毒性，并且在某些细胞系中随着时间的推移而增加，但这些影响在细胞系之间并不一致，并且似乎与晶体结构或测量的任何特定物理特性（包括大小、电荷和表面组成）无关，也与活性氧的产生无关。

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引用次数: 0

Effect of carbon chain length and concentration of perfluorinated compounds on polytetrafluoroethylene microplastics transport behavior 碳链长度和全氟化合物浓度对聚四氟乙烯微塑料输运行为的影响

IF 4.7 3区环境科学与生态学 Q2 ENVIRONMENTAL SCIENCES

NanoImpact

Pub Date : 2025-01-01 DOI: 10.1016/j.impact.2025.100550

Shihao Zhao , Ruihao Xu , Xiangying Liu , Yifan Wang , Yanji Jiang

Perfluorooctanoic acid (PFOA) and perfluoropentanoic acid (PFPeA), as important components of perfluorinated compounds (PFAS), are not only ecologically hazardous, but also have surfactant properties that can alter the transport behavior of polytetrafluoroethylene (PTFE) in porous media. In this experiment, the effect of PFAS on the transport of PTFE in porous media under different pH, ionic strength (IS) and ion valence states was studied. The results showed that the recovery rate of PTFE decreased gradually with the decrease of pH and the increase of IS and ion valence states. When the above conditions change, the double electron layer on the microplastic surface is compressed, the absolute value of zeta potential decreases, the repulsion between each other decreases, and aggregation and deposition are more likely. In addition, it was found that the recovery rate of PTFE co-transported with long chain PFOA was higher than that of short chain PFPeA. This phenomenon may be caused by the adhesion ability of PFOA with long carbon chain on the surface of PTFE is greater than that of PFPeA with short carbon chain. On the other hand, PFAS with different carbon chain lengths produce different spatial site resistance effects after binding with particles, and the spatial site resistance produced by the long-chain PFOA is larger than that of the short-chain PFPeA, leading to a decrease in particle-to-particle aggregation and a better transport effect. This study will help to understand the effects of PFAS with different carbon chain lengths on the transport of microplastics in porous media, as well as the transport rule of PTFE under different conditions, and provide reference value for the calculation of its flux in soil.

全氟辛酸（PFOA）和全氟戊酸（PFPeA）作为全氟化合物（PFAS）的重要组成部分，不仅具有生态危害性，而且具有表面活性剂的性质，可以改变聚四氟乙烯（PTFE）在多孔介质中的迁移行为。本实验研究了不同pH、离子强度（IS）和离子价态下PFAS对PTFE在多孔介质中输运的影响。结果表明，PTFE的回收率随着pH值的降低、IS价态和离子价态的增加而逐渐降低。当上述条件发生变化时，微塑性表面的双电子层受到压缩，zeta电位绝对值减小，相互之间的斥力减小，更容易发生聚集和沉积。此外，还发现PTFE与长链PFOA共输的回收率高于短链PFPeA。这种现象可能是由于长碳链的PFOA在PTFE表面的粘附能力大于短碳链的PFPeA。另一方面，不同碳链长度的PFAS与颗粒结合后产生不同的空间位点阻力效应，长链PFOA产生的空间位点阻力大于短链PFPeA，导致颗粒间聚集减少，转运效果更好。本研究将有助于了解不同碳链长度的PFAS对微塑料在多孔介质中输运的影响，以及不同条件下PTFE的输运规律，为计算其在土壤中的通量提供参考价值。

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引用次数: 0

Predicting the dissolution of metal-based nanoparticles by means of QSPRs and the effect of data augmentation 利用QSPRs预测金属基纳米颗粒的溶解及数据增强效应

IF 4.7 3区环境科学与生态学 Q2 ENVIRONMENTAL SCIENCES

NanoImpact

Pub Date : 2025-01-01 DOI: 10.1016/j.impact.2025.100547

Yuchao Song , Surendra Balraadjsing , Willie J.G.M. Peijnenburg , Martina G. Vijver

Particle dissolution is a critical process in the environmental fate assessment of metal-based nanoparticles (MNPs). Numerous attempts have been made previously to adequately quantify dissolution (kinetics), however, existing dissolution data and models are generally limited to a few nanomaterials or specific time points. Hence, they only capture phases of the process. This study aimed to develop a Quantitative Structure-Property Relationship (QSPR) model to predict the ion release (in %) of MNPs for different time points and water chemistry conditions. Furthermore, many machine learning models are frequently plagued by a lack of data and recently data augmentation has been suggested as a method to mitigate this issue. Therefore, we also investigated the effects of data augmentation on QSPRs. Following data collection from literature, QSPR models were generated and results indicate models with adequate performance (R² > 0.7). Results also demonstrated significant improvements in model performance with increasing amounts of applied data augmentation. However, a deeper evaluation of the results also highlighted that data augmentation can lead to misleading and overoptimistic model evaluation. Thus, proper model assessment is necessary when evaluating QSPRs. Variable importance analysis results revealed that the “initial concentration” and features related to the size and shape of MNPs were the most critical factors in the dissolution process. The predictive models generated here for MNP dissolution can improve nanomaterial testing efficiency and guide experimental design.

颗粒溶解是金属基纳米颗粒环境命运评价的关键过程。以前已经进行了许多尝试来充分量化溶解（动力学），然而，现有的溶解数据和模型通常仅限于几种纳米材料或特定的时间点。因此，它们只捕获流程的各个阶段。本研究旨在建立定量结构-性质关系（QSPR）模型来预测MNPs在不同时间点和水化学条件下的离子释放（in %）。此外，许多机器学习模型经常受到缺乏数据的困扰，最近数据增强被建议作为缓解这一问题的方法。因此，我们还研究了数据扩增对QSPRs的影响。通过文献资料收集，我们建立了QSPR模型，结果表明模型具有足够的性能(R2 >；0.7)。结果还表明，随着应用数据增强量的增加，模型性能得到了显著改善。然而，对结果进行更深入的评估也强调，数据增强可能导致误导性和过于乐观的模型评估。因此，在评价QSPRs时，适当的模型评估是必要的。变量重要性分析结果显示，“初始浓度”以及与MNPs大小和形状相关的特征是影响溶解过程的最关键因素。本文建立的MNP溶解预测模型可以提高纳米材料测试效率，指导实验设计。

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引用次数: 0