Pub Date : 2022-12-22DOI: 10.1163/15685411-bja10208
J. Gu, W. Ye, M. Munawar
A new species of Xiphinema americanum group was recovered in the rhizosphere of holly (Ilex purpurea) imported from Japan. It is described and illustrated in the present study as X. purpureum n. sp. The new species is characterised by moderately long females 2267 (2115-2550) μm, round lip region slightly offset from the rest of the body, 103.5 (98.5-109.0) μm long odontostyle, genital branches without discernible endosymbiotic bacteria, vulva located at 54.1 (51.6-57.1)% of the body, tail 30 (24.3-33.4) μm long with a broadly rounded terminus, male absent and four juvenile developmental stages. The polytomous codes of the new species are as follows: A4/5, B2/3, C1/2, D2/3, E3, F1, G1, H2, I1/2/3. The new species was molecularly characterised using near full length 18S, ITS1, and 28S D2-D3 regions of rRNA, and mitochondrial cytochrome c oxidase subunit I gene sequences. The phylogenetic analyses placed X. purpureum n. sp. with X. americanum group species, particularly with X. brevicolle complex species. This is the third X. americanum group species described from Japan.
{"title":"Description of Xiphinema pupureum n. sp. (Nematoda: Longidoridae), a new Xiphinema americanum group species detected from the rhizosphere of Ilex purpurea from Japan","authors":"J. Gu, W. Ye, M. Munawar","doi":"10.1163/15685411-bja10208","DOIUrl":"https://doi.org/10.1163/15685411-bja10208","url":null,"abstract":"\u0000A new species of Xiphinema americanum group was recovered in the rhizosphere of holly (Ilex purpurea) imported from Japan. It is described and illustrated in the present study as X. purpureum n. sp. The new species is characterised by moderately long females 2267 (2115-2550) μm, round lip region slightly offset from the rest of the body, 103.5 (98.5-109.0) μm long odontostyle, genital branches without discernible endosymbiotic bacteria, vulva located at 54.1 (51.6-57.1)% of the body, tail 30 (24.3-33.4) μm long with a broadly rounded terminus, male absent and four juvenile developmental stages. The polytomous codes of the new species are as follows: A4/5, B2/3, C1/2, D2/3, E3, F1, G1, H2, I1/2/3. The new species was molecularly characterised using near full length 18S, ITS1, and 28S D2-D3 regions of rRNA, and mitochondrial cytochrome c oxidase subunit I gene sequences. The phylogenetic analyses placed X. purpureum n. sp. with X. americanum group species, particularly with X. brevicolle complex species. This is the third X. americanum group species described from Japan.","PeriodicalId":18928,"journal":{"name":"Nematology","volume":" ","pages":""},"PeriodicalIF":1.2,"publicationDate":"2022-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46785041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-22DOI: 10.1163/15685411-bja10216
A. Ryss, K. S. Polyanina
The copulatory organs and pharynx are important for nematode classification. This research aimed to study the musculature of the wood-inhabiting nematode, Rhabditolaimus ulmi, using confocal microscopy. The lip region is hexagonal with enlarged lateral lips; the pharynx is diplogastrid type with two bulbs. The corpus comprises the fused stegostoma, procorpus and metacorpus, with six glands opening in the stegostoma and the corpus limen. The stegostoma has a large dorsal bulge and the posterior bulb is devoid of valve, with two circles of dilators and a basal sphincter. The female genital muscles have a four-radial symmetry around the vaginal opening to the uterus. Two vaginal constrictors and four pairs of dilators of two types are connected to the vagina-uterus opening; four dilators run perpendicular to the uterus axis and are connected to the uterine muscular corset. The sphincter between the intestine and cloaca consists of the middle ring with two pairs of processes connected ventrally with intestinal fasciculi and dorsally with the body wall. The ring contains the inner biconical X-structure. In the male, the gubernaculum has the dorsal erector and a pair of transverse muscles. The wide gubernacular spicule protractor envelops spicules anteriorly. The ventral tail muscle is wide, unpaired, and nine pairs of serial diagonal muscles are located beneath the body wall muscles, followed by three pairs of postcloacal oblique muscles of the tail. Anal depressors comprise a pair of transverse muscles with expanded middle globular parts. The copulatory muscles are of the Rhabditidae and Cephalobidae type modified because of spicules elongation. The muscle characters code of the previously published tabular key for 15 muscle characters is 73422-22144-42223; it can be used for phylogenetic reconstructions.
{"title":"The pharyngeal and genitalia-associated muscles of Rhabditolaimus ulmi (Nematoda: Chromodorea: Rhabditida: Diplogastridae)","authors":"A. Ryss, K. S. Polyanina","doi":"10.1163/15685411-bja10216","DOIUrl":"https://doi.org/10.1163/15685411-bja10216","url":null,"abstract":"\u0000The copulatory organs and pharynx are important for nematode classification. This research aimed to study the musculature of the wood-inhabiting nematode, Rhabditolaimus ulmi, using confocal microscopy. The lip region is hexagonal with enlarged lateral lips; the pharynx is diplogastrid type with two bulbs. The corpus comprises the fused stegostoma, procorpus and metacorpus, with six glands opening in the stegostoma and the corpus limen. The stegostoma has a large dorsal bulge and the posterior bulb is devoid of valve, with two circles of dilators and a basal sphincter. The female genital muscles have a four-radial symmetry around the vaginal opening to the uterus. Two vaginal constrictors and four pairs of dilators of two types are connected to the vagina-uterus opening; four dilators run perpendicular to the uterus axis and are connected to the uterine muscular corset. The sphincter between the intestine and cloaca consists of the middle ring with two pairs of processes connected ventrally with intestinal fasciculi and dorsally with the body wall. The ring contains the inner biconical X-structure. In the male, the gubernaculum has the dorsal erector and a pair of transverse muscles. The wide gubernacular spicule protractor envelops spicules anteriorly. The ventral tail muscle is wide, unpaired, and nine pairs of serial diagonal muscles are located beneath the body wall muscles, followed by three pairs of postcloacal oblique muscles of the tail. Anal depressors comprise a pair of transverse muscles with expanded middle globular parts. The copulatory muscles are of the Rhabditidae and Cephalobidae type modified because of spicules elongation. The muscle characters code of the previously published tabular key for 15 muscle characters is 73422-22144-42223; it can be used for phylogenetic reconstructions.","PeriodicalId":18928,"journal":{"name":"Nematology","volume":" ","pages":""},"PeriodicalIF":1.2,"publicationDate":"2022-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46448990","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-22DOI: 10.1163/15685411-bja10210
Chenying Zhang, M. Munawar, P. Castillo, Shousheng Han, Jingwu Zheng
Hemicycliophora species are migratory root ectoparasites of higher plants, commonly known as sheath nematodes. The present study describes a new species, Hemicycliophora geraerti n. sp., recovered from the rhizosphere of peach tree (Prunus persica) in Zhejiang Province, China. This species is characterised by a cuticular sheath loosely fitting to the body, a lip region slightly set-off with body contour bearing 3-4 annuli, oral plate narrow, ovate, and non-protruding, amphidial apertures plugged, dorsoventrally flanked in the first lip annulus, stylet 110 (103-116) μm long and flexible, excretory pore located 4-7 annuli posterior to the base of the pharyngeal bulb; vulval lips modified with a small vulval sleeve, anus located 16 (13-21) annuli posterior to vulva, tail elongated, having a narrowly rounded slightly offset spike. The molecular characterisation and phylogenetic analyses based on D2-D3 expansion segments of the 28S rRNA, ITS rRNA, and COI mtDNA gene indicated the unique status and support the novelty of this population of Hemicycliophora, therefore herein described as H. geraerti n. sp.
半环线虫是高等植物的迁移根外寄生虫，俗称鞘线虫。本文报道了浙江省桃树(Prunus persica)根际中发现的一新种——半环蚜(Hemicycliophora geraerti n. sp.)。本种的特征是角质层鞘与身体松散贴合，唇部略偏置，身体轮廓有3-4个环，口板狭窄，卵形，不突出，两侧孔塞住，第一个唇环背腹两侧，花柱长110 (103-116)μm，柔韧，排泄孔位于咽球基部后4-7个环;外阴唇具一小外阴套，肛门位于外阴后16(13-21)环空，尾部拉长，具一狭圆稍偏置的穗状突起。基于28S rRNA、ITS rRNA和COI mtDNA基因D2-D3扩增片段的分子特征和系统发育分析表明，该群体具有独特的地位，并支持其新颖性，因此本文将其命名为H. geraerti n. sp。
{"title":"Description of Hemicycliophora geraerti n. sp. (Tylenchida: Hemicycliophoridae) associated with Prunus persica L. from China","authors":"Chenying Zhang, M. Munawar, P. Castillo, Shousheng Han, Jingwu Zheng","doi":"10.1163/15685411-bja10210","DOIUrl":"https://doi.org/10.1163/15685411-bja10210","url":null,"abstract":"\u0000Hemicycliophora species are migratory root ectoparasites of higher plants, commonly known as sheath nematodes. The present study describes a new species, Hemicycliophora geraerti n. sp., recovered from the rhizosphere of peach tree (Prunus persica) in Zhejiang Province, China. This species is characterised by a cuticular sheath loosely fitting to the body, a lip region slightly set-off with body contour bearing 3-4 annuli, oral plate narrow, ovate, and non-protruding, amphidial apertures plugged, dorsoventrally flanked in the first lip annulus, stylet 110 (103-116) μm long and flexible, excretory pore located 4-7 annuli posterior to the base of the pharyngeal bulb; vulval lips modified with a small vulval sleeve, anus located 16 (13-21) annuli posterior to vulva, tail elongated, having a narrowly rounded slightly offset spike. The molecular characterisation and phylogenetic analyses based on D2-D3 expansion segments of the 28S rRNA, ITS rRNA, and COI mtDNA gene indicated the unique status and support the novelty of this population of Hemicycliophora, therefore herein described as H. geraerti n. sp.","PeriodicalId":18928,"journal":{"name":"Nematology","volume":" ","pages":""},"PeriodicalIF":1.2,"publicationDate":"2022-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44549174","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-20DOI: 10.1163/15685411-bja10212
E. Ivanova, M. Clausi, D. Leone, S. Spiridonov
A new nematode species, Phasmarhabditis villasmundi sp. n., was isolated from land gastropods in the Villasmundo-S. Alfio Nature Reserve in Syracuse Province, Sicily. Phasmarhabditis villasmundi sp. n. is characterised by the presence of males and females in the population and is recognised mainly by a conical female tail with prominent phasmids at its mid-length, spicules ca 82 μm long with a hole at the distal tip, infective juveniles with an average length of 773 μm and the distinct molecular characteristics of the new species. It is the second member of the genus described from Italy apart from P. apuliae Nermut’ et al., 2016 and is morphologically closest to this species. However, phylogenetic analyses based on ITS and D2-D3 LSU placed P. villasmundi sp. n. close to European P. neopapillosa and P. hermaphrodita, while P. apuliae appeared to be related to P. bohemica and the New World’s P. californica.
{"title":"Phasmarhabditis villasmundi sp. n. infecting land gastropods in the Nature Reserve ‘Speleological Complex Villasmundo – S. Alfio’ in Syracuse Province, Sicily","authors":"E. Ivanova, M. Clausi, D. Leone, S. Spiridonov","doi":"10.1163/15685411-bja10212","DOIUrl":"https://doi.org/10.1163/15685411-bja10212","url":null,"abstract":"\u0000A new nematode species, Phasmarhabditis villasmundi sp. n., was isolated from land gastropods in the Villasmundo-S. Alfio Nature Reserve in Syracuse Province, Sicily. Phasmarhabditis villasmundi sp. n. is characterised by the presence of males and females in the population and is recognised mainly by a conical female tail with prominent phasmids at its mid-length, spicules ca 82 μm long with a hole at the distal tip, infective juveniles with an average length of 773 μm and the distinct molecular characteristics of the new species. It is the second member of the genus described from Italy apart from P. apuliae Nermut’ et al., 2016 and is morphologically closest to this species. However, phylogenetic analyses based on ITS and D2-D3 LSU placed P. villasmundi sp. n. close to European P. neopapillosa and P. hermaphrodita, while P. apuliae appeared to be related to P. bohemica and the New World’s P. californica.","PeriodicalId":18928,"journal":{"name":"Nematology","volume":" ","pages":""},"PeriodicalIF":1.2,"publicationDate":"2022-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48907621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-16DOI: 10.1163/15685411-bja10209
Shiva Behzadian, N. Sahebani, Sepehr Karimi
Suppression of plant defence systems by plant-parasitic nematodes is an important aspect of research on resistance induction in plants. The present work aimed to address the question of whether this suppressive ability by nematodes can be spread systemically and equally to other parts of the plant. The root-knot nematode, Meloidogyne javanica, and the host plant, tomato, was used with salicylic acid (SA) as a potent inducer of the plant defence systems. SA was sprayed on the second and fifth leaves, as near and far from the nematode establishment site, respectively. The results of hydrogen peroxide and phenylalanine ammonia lyase enzyme evaluation in the root (site of nematode establishment), crown, second and fifth leaves showed that, firstly, the amount of defence compounds induced by SA in the leaves was not equal in all parts of the plant and gradually decreased from the aerial parts (treatment site) towards the roots. Furthermore, the synthesis and accumulation of the evaluated compounds in the younger parts of the plant (fifth leaf) was more than the older parts (second leaf), and the crown and root. Although the suppression of the plant defence systems by the nematode was transferred to other parts of the plant, the amount of this suppression gradually decreased in the parts farthest from the site of the nematode establishment. Based on the results, it is inferred that the success of a resistance induction strategy in plant disease management depends on the type of pathogen, type of inducer, place of application of the inducer on the plant and the induction area.
{"title":"Relationship between suppression of plant defence systems by Meloidogyne javanica with the distance of different parts of the plant from the nematode establishment site","authors":"Shiva Behzadian, N. Sahebani, Sepehr Karimi","doi":"10.1163/15685411-bja10209","DOIUrl":"https://doi.org/10.1163/15685411-bja10209","url":null,"abstract":"\u0000Suppression of plant defence systems by plant-parasitic nematodes is an important aspect of research on resistance induction in plants. The present work aimed to address the question of whether this suppressive ability by nematodes can be spread systemically and equally to other parts of the plant. The root-knot nematode, Meloidogyne javanica, and the host plant, tomato, was used with salicylic acid (SA) as a potent inducer of the plant defence systems. SA was sprayed on the second and fifth leaves, as near and far from the nematode establishment site, respectively. The results of hydrogen peroxide and phenylalanine ammonia lyase enzyme evaluation in the root (site of nematode establishment), crown, second and fifth leaves showed that, firstly, the amount of defence compounds induced by SA in the leaves was not equal in all parts of the plant and gradually decreased from the aerial parts (treatment site) towards the roots. Furthermore, the synthesis and accumulation of the evaluated compounds in the younger parts of the plant (fifth leaf) was more than the older parts (second leaf), and the crown and root. Although the suppression of the plant defence systems by the nematode was transferred to other parts of the plant, the amount of this suppression gradually decreased in the parts farthest from the site of the nematode establishment. Based on the results, it is inferred that the success of a resistance induction strategy in plant disease management depends on the type of pathogen, type of inducer, place of application of the inducer on the plant and the induction area.","PeriodicalId":18928,"journal":{"name":"Nematology","volume":" ","pages":""},"PeriodicalIF":1.2,"publicationDate":"2022-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44769735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-02DOI: 10.1163/15685411-bja10202
Motonori Takagi, S. Sekimoto, T. Mizukubo, David Wari, M. Akiba, R. Perry, K. Toyota
Hirschmanniella diversa and H. imamuri parasitise the lotus, Nelumbo nucifera. Hirschmanniella spp. are the most important pests of lotus cultivation in Japan, but the distribution and species dominancy are not fully understood. In this study, we aimed to collect Hirschmanniella spp. individuals from lotus roots in the major lotus cultivation areas in Japan and identify them morphologically and molecularly using the 18S, D2-D3 regions of rRNA and mitochondrial cytochrome c oxidase subunit I (mtCOI). Hirschmanniella oryzae, commonly known to cause damage on rice in Japan, was also obtained and analysed for inter- and intraspecies sequence variations. All individuals of the genus Hirschmanniella extracted from lotus roots in 16 fields in ten prefectures were identified as H. diversa morphologically and molecularly. This result revealed that H. diversa is a dominant Hirschmanniella species parasitising lotus in Japan. Meanwhile, intraspecific sequence variations in H. diversa were relatively higher in the mtCOI (1-21 bases, ≦5.3%) than in the 18S rRNA and D2-D3 of 28S rRNA (0.0-0.3% and 0.0-0.4%, respectively) and in those of H. oryzae. Based on the mtCOI sequences, five haplotypes were identified and each haplotype was distributed in relatively close regions or regions in which the same lotus cultivars were grown, although further studies are required to verify this assertion. The morphological and genetic variations were higher in H. diversa than in H. oryzae (no intraspecific sequence variation in mtCOI), suggesting that H. diversa was not a recent invasive species.
{"title":"Geographical distribution and phylogenic relationships of Hirschmanniella diversa Sher (Nematoda: Pratylenchidae) in Japan","authors":"Motonori Takagi, S. Sekimoto, T. Mizukubo, David Wari, M. Akiba, R. Perry, K. Toyota","doi":"10.1163/15685411-bja10202","DOIUrl":"https://doi.org/10.1163/15685411-bja10202","url":null,"abstract":"\u0000Hirschmanniella diversa and H. imamuri parasitise the lotus, Nelumbo nucifera. Hirschmanniella spp. are the most important pests of lotus cultivation in Japan, but the distribution and species dominancy are not fully understood. In this study, we aimed to collect Hirschmanniella spp. individuals from lotus roots in the major lotus cultivation areas in Japan and identify them morphologically and molecularly using the 18S, D2-D3 regions of rRNA and mitochondrial cytochrome c oxidase subunit I (mtCOI). Hirschmanniella oryzae, commonly known to cause damage on rice in Japan, was also obtained and analysed for inter- and intraspecies sequence variations. All individuals of the genus Hirschmanniella extracted from lotus roots in 16 fields in ten prefectures were identified as H. diversa morphologically and molecularly. This result revealed that H. diversa is a dominant Hirschmanniella species parasitising lotus in Japan. Meanwhile, intraspecific sequence variations in H. diversa were relatively higher in the mtCOI (1-21 bases, ≦5.3%) than in the 18S rRNA and D2-D3 of 28S rRNA (0.0-0.3% and 0.0-0.4%, respectively) and in those of H. oryzae. Based on the mtCOI sequences, five haplotypes were identified and each haplotype was distributed in relatively close regions or regions in which the same lotus cultivars were grown, although further studies are required to verify this assertion. The morphological and genetic variations were higher in H. diversa than in H. oryzae (no intraspecific sequence variation in mtCOI), suggesting that H. diversa was not a recent invasive species.","PeriodicalId":18928,"journal":{"name":"Nematology","volume":" ","pages":""},"PeriodicalIF":1.2,"publicationDate":"2022-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43559266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-11-23DOI: 10.1163/15685411-bja10213
S. Azimi, Abbas Abdolkhani
Ditylenchus pedrami n. sp., recovered from the rhizospheric soil of date palm in Khuzestan province, southwest Iran, is described and illustrated based upon morphological and molecular data. The new species is characterised by having six lines in the lateral field, lip region smooth and continuous with body contour, stylet 9-11 μm long, median pharyngeal bulb oval with small valve, pharyngeal bulb offset from the intestine, V = 83.9 (80.2-88.1), conoid tail with a pointed, dull or rounded tip and males with 20.1 (17-24) μm long spicules. Morphologically, the new species comes close to D. africanus, D. anchilisposomus, D. australiae, D. clarus, D. clavicaudatus and D. parcevivens, mainly by having shared features like six lines in the lateral fields, stylet length and somewhat similar tail tip. The new species was also compared with D. stenurus and D. sarvarae, two species with close phylogenetic affinities to it. The phylogenetic relationships of the new species with representatives of the family Anguinidae were reconstructed and discussed using partial sequences of the small subunit, D2-D3 expansion segments of the large subunit, and internal transcribed spacer regions of ribosomal DNA (SSU, LSU D2-D3 and ITS rDNA) based on Bayesian inference (BI).
{"title":"Description and molecular characterisation of Ditylenchus pedrami n. sp. (Rhabditida: Anguinidae) from Iran","authors":"S. Azimi, Abbas Abdolkhani","doi":"10.1163/15685411-bja10213","DOIUrl":"https://doi.org/10.1163/15685411-bja10213","url":null,"abstract":"\u0000 Ditylenchus pedrami n. sp., recovered from the rhizospheric soil of date palm in Khuzestan province, southwest Iran, is described and illustrated based upon morphological and molecular data. The new species is characterised by having six lines in the lateral field, lip region smooth and continuous with body contour, stylet 9-11 μm long, median pharyngeal bulb oval with small valve, pharyngeal bulb offset from the intestine, V = 83.9 (80.2-88.1), conoid tail with a pointed, dull or rounded tip and males with 20.1 (17-24) μm long spicules. Morphologically, the new species comes close to D. africanus, D. anchilisposomus, D. australiae, D. clarus, D. clavicaudatus and D. parcevivens, mainly by having shared features like six lines in the lateral fields, stylet length and somewhat similar tail tip. The new species was also compared with D. stenurus and D. sarvarae, two species with close phylogenetic affinities to it. The phylogenetic relationships of the new species with representatives of the family Anguinidae were reconstructed and discussed using partial sequences of the small subunit, D2-D3 expansion segments of the large subunit, and internal transcribed spacer regions of ribosomal DNA (SSU, LSU D2-D3 and ITS rDNA) based on Bayesian inference (BI).","PeriodicalId":18928,"journal":{"name":"Nematology","volume":" ","pages":""},"PeriodicalIF":1.2,"publicationDate":"2022-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46484037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-11-23DOI: 10.1163/15685411-bja10207
L. Taning, L. Chann, S. Fleerakkers, N. Cap, E. Formesyn, L. Tirry, W. Wesemael
Problems with plant-parasitic nematodes remain a major challenge in most field vegetable crops worldwide. In agricultural fields in Belgium, these problems have increased in recent years. A major problem is the quarantine root-knot nematode Meloidogyne chitwoodi. The host plant status of the most commonly grown cultivars of spinach (Spinacia oleracea) and the damage threshold for M. chitwoodi were determined in a climate-controlled glasshouse and in a naturally infested field. The yield data with the final population densities were fitted to Seinhorst’s yield and population dynamic models, respectively. The damage threshold values (T) for the relative fresh weight yield were 0.14, 1.08, 11.53 J2 (100 cm3 soil)−1, minimum yield (m) of 0.84, 0.64, 0.52 and maximum yield () of 12.51, 12.44 and 9.60 for ‘Gnu’, ‘Meerkat’ and ‘Whale’ spinach cultivars, respectively. The maximum multiplication rates (a) were 3.81, 2.38 and 2.72, whilst the maximum population densities (M) were 563, 916, and 922 J2 (100 cm3 soil)−1 for ‘Gnu’, ‘Meerkat’ and ‘Whale’, respectively. Meloidogyne chitwoodi had an impact on the spinach yield. The higher the nematode density the lower the yield and vice versa. The tested cultivars were all hosts for M. chitwoodi but differences between cultivars occurred. ‘Kolibri’ and ‘Rhino’ were tested as the least sensitive cultivars for M. chitwoodi during the host screening test and bring opportunities for resistance breeding. This is the first report of spinach as a host for M. chitwoodi.
{"title":"Host plant status and damage threshold of spinach (Spinacia oleracea) for the temperate root-knot nematode Meloidogyne chitwoodi","authors":"L. Taning, L. Chann, S. Fleerakkers, N. Cap, E. Formesyn, L. Tirry, W. Wesemael","doi":"10.1163/15685411-bja10207","DOIUrl":"https://doi.org/10.1163/15685411-bja10207","url":null,"abstract":"\u0000Problems with plant-parasitic nematodes remain a major challenge in most field vegetable crops worldwide. In agricultural fields in Belgium, these problems have increased in recent years. A major problem is the quarantine root-knot nematode Meloidogyne chitwoodi. The host plant status of the most commonly grown cultivars of spinach (Spinacia oleracea) and the damage threshold for M. chitwoodi were determined in a climate-controlled glasshouse and in a naturally infested field. The yield data with the final population densities were fitted to Seinhorst’s yield and population dynamic models, respectively. The damage threshold values (T) for the relative fresh weight yield were 0.14, 1.08, 11.53 J2 (100 cm3 soil)−1, minimum yield (m) of 0.84, 0.64, 0.52 and maximum yield () of 12.51, 12.44 and 9.60 for ‘Gnu’, ‘Meerkat’ and ‘Whale’ spinach cultivars, respectively. The maximum multiplication rates (a) were 3.81, 2.38 and 2.72, whilst the maximum population densities (M) were 563, 916, and 922 J2 (100 cm3 soil)−1 for ‘Gnu’, ‘Meerkat’ and ‘Whale’, respectively. Meloidogyne chitwoodi had an impact on the spinach yield. The higher the nematode density the lower the yield and vice versa. The tested cultivars were all hosts for M. chitwoodi but differences between cultivars occurred. ‘Kolibri’ and ‘Rhino’ were tested as the least sensitive cultivars for M. chitwoodi during the host screening test and bring opportunities for resistance breeding. This is the first report of spinach as a host for M. chitwoodi.","PeriodicalId":18928,"journal":{"name":"Nematology","volume":" ","pages":""},"PeriodicalIF":1.2,"publicationDate":"2022-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47195104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-11-23DOI: 10.1163/15685411-bja10205
A. Calandrelli, M. T. Silva, A. Miamoto, L. Rinaldi, L. Favoreto, M. C. Meyer, A. C. Machado, S. Silva, C. R. Dias-Arieira
Aphelenchoides besseyi is a concern to Brazilian agriculture, since the losses caused by the green stem and foliar retention syndrome in soybean, especially in hot and humid climates, could reach high levels. However, limited information is available regarding the host-parasite relationship. The objective of this study was to evaluate the capacity of A. besseyi to penetrate soybean roots and its movement in soybean tissues until reaching the top parts. Plants inoculated with 1000 A. besseyi were evaluated for root penetration from 1-28 days after inoculation (DAI), and post-penetration and movement inside tissues by staining them with acid fuchsin. Plant development, chronology of the symptoms and the number of pods were also evaluated at 60 DAI. Aphelenchoides besseyi was found in the cortex and xylem vessels of roots from 1 DAI. During the experimental period, the number of nematodes from roots to shoots increased more than 123, 330 and 430%, respectively, for roots, stems + petioles + inflorescences, and leaves, demonstrating the ascendent movement of A. besseyi. Necrotic lesions in young leaves and apical tissues were observed from 8 DAI; the typical symptom of ‘amachamiento’ was observed from 10 DAI, with enlargement of nodes and lesions in inflorescences and, at 30 DAI, stunted plants were detected. At 60 DAI, inoculated plants had lower numbers of pods and exhibited severe symptoms including abortion of flowers and tissue splitting and cortical lesions in a few pods and roots. These observations emphasise the importance of A. besseyi for Brazilian soybean production.
{"title":"Host-parasite relationship between Aphelenchoides besseyi and soybean","authors":"A. Calandrelli, M. T. Silva, A. Miamoto, L. Rinaldi, L. Favoreto, M. C. Meyer, A. C. Machado, S. Silva, C. R. Dias-Arieira","doi":"10.1163/15685411-bja10205","DOIUrl":"https://doi.org/10.1163/15685411-bja10205","url":null,"abstract":"\u0000Aphelenchoides besseyi is a concern to Brazilian agriculture, since the losses caused by the green stem and foliar retention syndrome in soybean, especially in hot and humid climates, could reach high levels. However, limited information is available regarding the host-parasite relationship. The objective of this study was to evaluate the capacity of A. besseyi to penetrate soybean roots and its movement in soybean tissues until reaching the top parts. Plants inoculated with 1000 A. besseyi were evaluated for root penetration from 1-28 days after inoculation (DAI), and post-penetration and movement inside tissues by staining them with acid fuchsin. Plant development, chronology of the symptoms and the number of pods were also evaluated at 60 DAI. Aphelenchoides besseyi was found in the cortex and xylem vessels of roots from 1 DAI. During the experimental period, the number of nematodes from roots to shoots increased more than 123, 330 and 430%, respectively, for roots, stems + petioles + inflorescences, and leaves, demonstrating the ascendent movement of A. besseyi. Necrotic lesions in young leaves and apical tissues were observed from 8 DAI; the typical symptom of ‘amachamiento’ was observed from 10 DAI, with enlargement of nodes and lesions in inflorescences and, at 30 DAI, stunted plants were detected. At 60 DAI, inoculated plants had lower numbers of pods and exhibited severe symptoms including abortion of flowers and tissue splitting and cortical lesions in a few pods and roots. These observations emphasise the importance of A. besseyi for Brazilian soybean production.","PeriodicalId":18928,"journal":{"name":"Nematology","volume":" ","pages":""},"PeriodicalIF":1.2,"publicationDate":"2022-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43376860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-11-18DOI: 10.1163/15685411-bja10214
R. Peña‐Santiago, Nazareth Cortés, M. García-Ruiz, J. Abolafia
A new species of the genus Talanema, T. ibericum sp. n., collected in natural areas of southeastern Iberian Peninsula, is characterised, including morphological description, line, LM and SEM illustrations, and molecular (18S, D2-D3 28S rDNA sequences) study. The new species is distinguished by its 1.27-1.59 mm long body, lip region offset by constriction and 14-16.5 μm wide with perioral liplets, odontostyle 16.5-19.5 μm long, neck 285-359 μm long, pharyngeal expansion occupying 42-48% of the total neck length, uterus tripartite and 147-228 μm long or 2.6-4.0 body diam., vulva transverse (V = 53-59), presence of cuticular irregularities at both sides of vulva, female tail conical with a dorsal concavity (27-40 μm, c = 36-54, c′ = 1.0-1.3), male tail short and rounded conoid (24 μm, c = 66, c′ = 0.8), spicules 58 μm long, and 17 almost contiguous ventromedian supplements with hiatus. Morphological and molecular data reveal that the new species is similar to (but easily separable from) T. baqrii, with which it is compared. Present results support the monophyly of the genus Talanema.
{"title":"Morphological and molecular characterisation of Talanema ibericum sp. n. (Dorylaimida, Qudsianematidae) from southern Iberian Peninsula","authors":"R. Peña‐Santiago, Nazareth Cortés, M. García-Ruiz, J. Abolafia","doi":"10.1163/15685411-bja10214","DOIUrl":"https://doi.org/10.1163/15685411-bja10214","url":null,"abstract":"\u0000 A new species of the genus Talanema, T. ibericum sp. n., collected in natural areas of southeastern Iberian Peninsula, is characterised, including morphological description, line, LM and SEM illustrations, and molecular (18S, D2-D3 28S rDNA sequences) study. The new species is distinguished by its 1.27-1.59 mm long body, lip region offset by constriction and 14-16.5 μm wide with perioral liplets, odontostyle 16.5-19.5 μm long, neck 285-359 μm long, pharyngeal expansion occupying 42-48% of the total neck length, uterus tripartite and 147-228 μm long or 2.6-4.0 body diam., vulva transverse (V = 53-59), presence of cuticular irregularities at both sides of vulva, female tail conical with a dorsal concavity (27-40 μm, c = 36-54, c′ = 1.0-1.3), male tail short and rounded conoid (24 μm, c = 66, c′ = 0.8), spicules 58 μm long, and 17 almost contiguous ventromedian supplements with hiatus. Morphological and molecular data reveal that the new species is similar to (but easily separable from) T. baqrii, with which it is compared. Present results support the monophyly of the genus Talanema.","PeriodicalId":18928,"journal":{"name":"Nematology","volume":" ","pages":""},"PeriodicalIF":1.2,"publicationDate":"2022-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42187109","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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