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Proposal of New Synonyms in Hemicycliophora De Man, 1921 (Nematoda: Tylenchida) 半圆线虫属(cycycliophora De Man, 1921)新同义词的提出(线虫纲:蛛形目)
Pub Date : 1998-01-01 DOI: 10.1163/005325998X00027
E. Costa-Manso
The comparative study of morphometrical variability and the evaluation of characters of para-types of eight Hemicycliophora species provided a basis for the proposal of four new synonyms in the genus. H. lutosoides, H. ekdavici, H. karachiensis and H. ferrisae are proposed as junior synonyms of H. lutosa, H. natalensis, H. typica and H. uniformis, respectively.
对8种半环子属植物的形态变异和准型特征进行了比较研究,为提出该属4个新近义词提供了依据。H. lutosoides、H. ekdavici、H. karachiensis和H. ferrisae分别被认为是H. lutosa、H. natalensis、H. typica和H. uniformis的初级同义词。
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引用次数: 2
Bursaphelenchus hellenicus sp. n. (Nematoda, Aphelenchoididae) from Greek pine wood.
Pub Date : 1998-01-01 DOI: 10.1163/005725998X00032
G. Skarmoutsos, H. Braasch, H. Michalopoulou
Bursaphelenchus hellenicus sp. n. was found in the wood of a dying Pinus brutia tree in Thessaloniki, Greece, together with B. leoni, in 1996. The tree was affected by Blastophagus piniperda and blue stain of wood. B. hellenicus sp. n. is characterised by an a-value of 30 (22-38), stylet length of 15 (13-17) μm, distinct constriction of the lip region, spicules of males with blunt prominent rostrum, narrow high condylus and cucullus, females with slightly extended anterior vulval lip, medium long postuterine branch and conoid tail with rounded terminus.
Bursaphelenchus hellenicus sp. n.于1996年在希腊塞萨洛尼基的一棵垂死的松树中与B. leoni一起被发现。该树主要受裂食糜虫(Blastophagus pininiperda)和木材蓝斑病的影响。其特征是a值为30(22-38),花柱长度为15 (13-17)μm,唇区明显收缩,雄性的针尖尖钝,突出的喙部,狭窄的高髁和冠部,雌性外阴前唇略伸长,子宫后分支中等长,尾圆锥形,末端圆形。
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引用次数: 24
Effects of crop rotations of cereals with vetch and fallow on soil nematofauna in Central Spain 西班牙中部谷地与野豌豆和休耕轮作对土壤线虫的影响
Pub Date : 1998-01-01 DOI: 10.1163/005225998X00073
G. Nombela, A. Navas, A. Bello
The influence of the use of vetch or fallow in rotation with cereals on the dynamics of nematode populations was studied in a field experiment conducted on a luvisol in Central Spain. All the nematodes present in the trial were monitored by seasonal samplings for three consecutive years, starting one year after the first rotation cycle was completed. Vetch in rotation with cereals controlled soil populations of some virus transmitters (Xiphinema pachtaicum and Trichodorus sp.) and the cyst nematode Heterodera avenae. Fallow also controlled X. pachtaicum and H. avenae, as well as Pratylenchus thornei, Ditylenchus dipsaci, Tylenchorhynchus maximus and Tylenchus sp. Other Dorylaimidae and Rhabditidae species seemed to be influenced by vetch and fallow only indirectly. Neither rotation influenced Longidorus profundorum populations. The maturity index based on the nematode fauna was used as a measure of the disturbance in this ecosystem.
在西班牙中部进行的一项野外试验中,研究了使用野豌豆或休耕轮作谷物对线虫种群动态的影响。从第一个轮作周期完成一年后开始,连续三年通过季节性取样监测试验中出现的所有线虫。与谷物轮作的紫菀控制了一些病毒传播者(刺线虫和毛霉)和包囊线虫的土壤种群。休耕地还能控制长尾蠓和大尾蠓,以及刺尾蠓、长尾蠓、大尾蠓和短尾蠓,而其他多尾蠓科和横纹蛇科物种似乎只受紫菀和休耕地的间接影响。这两种轮作都不影响深沟长蛾种群。以线虫区系为基础的成熟度指数作为该生态系统扰动的衡量指标。
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引用次数: 16
Control of the root-knot nematode Meloidogyne javanica by the parasite Pasteuria penetrans as influenced by the initial nematode population densities. 穿透性巴氏菌对javanmeloidogyne根结线虫的控制受初始线虫种群密度的影响。
Pub Date : 1998-01-01 DOI: 10.1163/005525998X00043
A. Channer, S. Gowen, E. Tzortzakakis
The potential of Pasteuria penetrans for the biological control of Meloidogyne javanica on tomato plants was examined in a pot experiment. P. penetrans in dried powdered roots was applied at 11000 and 55 000 spores/cm 3 soil in pots inoculated with 420 or 4200 second stage juveniles. At 4 months after planting there was significantly less galling on plants grown in pots inoculated with 420 nematodes and 55 000 spores/cm 3 than in the unamended control pots. There was a 96% decrease in numbers of egg masses on these roots. The period of assessment had to be shortened because the control plants in the treatment with the higher nematode inoculum died prematurely curtailing further reproduction. Treatment with P. penetrans prevented premature senescence of the plants allowing nematode reproduction to continue. A significant interaction between P. penetrans density and nematode density was obtained on the number of juveniles in soil after the first crop. Where the nematode population was initially large (4200/pot), there were more juveniles in the treatments to which P. penetrans had been applied than in the untreated controls. However, where the nematode population was initially small (420/pot), there were more juveniles in the untreated control than in the treatment with the higher dose of P. penetrans. A second crop was grown in soil from the different treatments after either removing or incorporating the dried roots of the preceding crop. After 30 days, root galling and egg production were higher in pots initially inoculated with 420 nematodes. The effect of root incorporation on egg production was influenced by both nematode and P. penetrans level; greater numbers of eggs were produced on treatments with the initially higher levels. It is concluded that P. penetrans, deployed as a single control measure, may not give consistent or durable control, but that it may have a role in an integrated approach to nematode management.
通过盆栽试验,探讨了穿透性巴氏菌在番茄蔓曲霉病防治中的应用潜力。在分别接种420和4200个二龄幼体的花盆中,分别在11000和55000孢子/cm 3的土壤中施用干燥粉末根中的穿山药。在种植后4个月,接种了420个线虫和55000个孢子/ cm3的盆栽中生长的植株的刺痛明显少于未接种的对照盆栽。这些根上的卵块数量减少了96%。由于较高线虫接种量处理的对照植株过早死亡,限制了进一步的繁殖,因此必须缩短评估周期。用穿透性假单胞菌处理可以防止植物过早衰老,从而使线虫继续繁殖。第一次收获后,穿透线虫密度和线虫密度对土壤幼体数量有显著的交互作用。在初始线虫数量较多(4200只/罐)的情况下,施透线虫处理的幼虫数量多于未施透线虫处理。然而,在初始线虫数量较少(420只/锅)的情况下,未处理的对照比高剂量渗透线虫处理的幼虫数量更多。第二种作物在除去或合并前一种作物的干根后,在不同处理的土壤中生长。30 d后,在初始接种420只线虫的盆栽中,根损和产蛋量较高。根系掺入对虫卵产量的影响受线虫和渗透线虫水平的影响;在初始水平较高的处理中,产生的卵子数量更多。综上所述,作为一种单一的控制措施,渗透线虫可能不能起到一致或持久的控制作用,但它可能在线虫管理的综合方法中发挥作用。
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引用次数: 15
Proceedings of the 24th International Nematology Symposium 第24届国际线虫学研讨会论文集
Pub Date : 1998-01-01 DOI: 10.1163/187529298X00015
R. Cook, M. Phillips
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引用次数: 0
Infectivity and Specificity of Canadian and Czech Isolates of Steinernema Kraussei (Steiner, 1923) To Some Insect Pests At Low Temperatures in the Laboratory 加拿大和捷克克劳斯斯坦内菌分离株(Steiner, 1923)在实验室低温下对某些害虫的传染性和特异性
Pub Date : 1998-01-01 DOI: 10.1163/005525998X00098
J. M. Webster, P. Kindlmann, S. Bečár, Z. Acek
The infectivity of steinernematid nematodes varies with temperature. Five insect species, namely codling moth, Cydia pomonella; mealworm, Tenebrio molitor; blow fly, Sarcophaga bullata; March fly, Bibio marci; and web spinning sawfly, Cephaleia abietis were exposed to a Canadian (# 76) isolate of Steinernema kraussei at 7°C. C. abietis was exposed to the Canadian isolate # D and Czech isolate Vimperk at 4°C. These experiments were done in Petri dishes filled with moist sand, exposure time was 250 h and inoculum ranged from 50 to 800 infective juveniles (IJs). The highest larval mortality occurred in lepidopteran and coleopteran species, C. pomonella (73-100%) and T. molitor (28-70%), respectively. Few dipteran larvae, S. bullata and B. marci, were killed. The mortality of C. abietis larvae ranged from 2% (isolate # 76, at 50 IJs/dish) to 30% (isolate Vimperk, at 400 IJs/dish) at 7°C, but was negligible at 4°C. The intensity of infection expressed as the number of adult nematodes recovered from the insect cadavers, was greatest in C. pomonella where it ranged from 16 to 25% of the initial IJ inoculum, but was almost zero for both fly larval species. When analysed and fitted to the modified Anderson host-parasite model, the data confirmed a significant level of Steinernema parasitism for C. abietis, T. molitor, and C. pomonella larvae at low temperatures, and showed that the two species of fly larvae tested were almost fully resistant to parasitism by S. kraussei. Natural adaptation of the Czech Vimperk isolate to C. abietis may have enhanced this isolate's infectivity when compared with that of the Canadian nematode isolates.
斯坦氏线虫的传染性随温度而变化。五种昆虫,分别为冷蛾、雨蛾;粉虫,黄粉虫;吹蝇(Sarcophaga bullata)三月飞,比翼双飞;将织网锯蝇(Cephaleia abietis)暴露于加拿大kraussei steinerma菌株(# 76)中,温度为7°C。C. abietis暴露于加拿大分离物# D和捷克分离物Vimperk,温度为4℃。实验在充满湿沙的培养皿中进行,暴露时间为250 h,接种量为50 ~ 800只感染幼虫。鳞翅目和鞘翅目的幼虫死亡率最高,分别为pomonella(73-100%)和molitor(28-70%)。双翅目幼虫bullata和B. marci鲜少被杀。在7°C时,冷杉棘球蚴幼虫的死亡率为2%(76号分离株,50 IJs/皿)至30% (Vimperk分离株,400 IJs/皿),但在4°C时可以忽略不计。从昆虫尸体中恢复的成线虫数量表示的感染强度在波蒙梭菌中最大,其范围为初始IJ接种量的16%至25%,但在两种蝇幼虫中几乎为零。通过对改进的Anderson宿主-寄生虫模型进行分析和拟合,数据证实了低温条件下abietis、T. molitor和C. pomonella三种蝇类幼虫的斯坦纳氏蝇寄生水平显著,并表明这两种蝇类幼虫对S. kraussei的寄生几乎完全具有抗性。与加拿大线虫分离株相比,捷克Vimperk分离株对杉树线虫的自然适应可能增强了该分离株的传染性。
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引用次数: 13
Biocontrol of Heterodera Schachtii Using Combinations of the Sterile Fungus, Stfch1-1, Embellisia Chlamydospora and Verticilli Um Chlamydospori Um
Pub Date : 1998-01-01 DOI: 10.1163/005525998X00025
A. Jalali, R. Segers, J. Coosemans
A sterile fungus, designated as StFCh1-1, and two other fungi, Embellisia chlamydospora and Verticillium chlamydosporium, isolated from cysts of the sugar beet cyst nematode, Heterodera schachtii, were tested as biological control agents against this nematode. The combination of StFCh1-1 with either E. chlamydospora or V. chlamydosporium resulted in significantly greater impact on the nematode population than any fungus alone. More females and cysts were colonised by StFCh1-1 than by either other antagonist. These nematodes showed the typical fungal sclerotium on the outside of the body wall, and the hyphal imprint on the inside of the cyst. Few females developed on sugar beet roots in the soil of those treatments that included the sterile fungus. No juveniles emerged from cysts colonised by the sterile fungus, while some juveniles hatched from eggs within cysts colonised by either E. chlamydospora or V. chlamydosporium. The density of E. chlamydospora and V. chlamydosporium in soil, as measured by colony forming unit counts, decreased over time. Quantification of the sterile fungus in soil was not possible using this technique due to rampant growth of saprophytic fungi on the isolation medium. The results suggest that, despite the promising nature of the sterile fungus StFCh1-1 in comparison with the two other fungi used, a carefully combined inoculum could still be more effective at controlling H. schachtii. The results are discussed in the context of compatibility of these organisms.
从甜菜包囊线虫沙氏异源线虫(Heterodera schachtii)的包囊中分离出一株无菌真菌StFCh1-1和另外两株真菌衣孢子润菌(renisia chlamydospora)和衣孢子黄萎病菌(Verticillium chlamydosporium)作为防治甜菜包囊线虫的生物制剂。StFCh1-1与E. chlamydospora或V. chlamydosporium组合对线虫种群的影响显著大于单独使用任何真菌。StFCh1-1比其他拮抗剂定殖更多的雌性和囊肿。这些线虫体壁外侧有典型的真菌菌核,囊肿内侧有菌丝印记。在含有无菌真菌的土壤中,很少有雌性在甜菜根上发育。由无菌真菌定殖的包囊中没有孵化出幼虫,而由衣孢子e菌或衣孢子v菌定殖的包囊内的卵孵化出一些幼虫。以菌落形成单位计数测定,土壤中衣孢子e和衣孢子v的密度随时间而降低。由于腐生真菌在分离培养基上生长猖獗,因此无法使用该技术对土壤中的无菌真菌进行定量。结果表明,尽管无菌真菌StFCh1-1与使用的其他两种真菌相比具有良好的性质,但精心组合的接种物仍然可以更有效地控制沙克蒂嗜血杆菌。结果在这些生物的相容性的背景下进行了讨论。
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引用次数: 11
Comparison of the Biology and Freezing Tolerance of Panagrolaimus Davidi, an Antarctic Nematode, From Field Samples and Cultures 从野外样品和培养物比较南极线虫的生物学和抗冻性
Pub Date : 1998-01-01 DOI: 10.1163/005725998X00050
D. Wharton
Samples of Panagrolaimus davidi, an Antarctic nematode which tolerates intracellular freezing, were collected from Cape Bird, Ross Island and compared with nematodes cultured for nine years. The field distribution of nematodes was patchy and was not correlated with the water content of the sample or the presence of algae. Field-collected nematodes were similar in morphology to those from cultures, although there were differences in morphometrics. Males predominated in field material, whereas cultures contained only parthenogenetic females. The freezing tolerance of field-collected nematodes was similar to those from cultures. Nematodes in culture are thus a valid model for the study of the adaptations involved in the survival of the extreme environmental stresses faced by these animals in their Antarctic habitats.
在罗斯岛的伯德角收集了一种能耐受细胞内冷冻的南极线虫,并将其与培养了9年的线虫进行了比较。线虫的野外分布是斑驳的,与样品的含水量或藻类的存在无关。野外采集的线虫在形态上与培养的线虫相似,尽管在形态计量学上存在差异。雄性在田间材料中占主导地位,而培养物中只含有孤雌生殖的雌性。田间采集的线虫的耐寒性与培养的相似。因此,培养的线虫是研究这些动物在南极栖息地所面临的极端环境压力下生存所涉及的适应性的有效模型。
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引用次数: 14
Loofilaimus, a Remarkable New Genus of Dorylaimida From Iran 伊朗多叶蝇属的一个新属
Pub Date : 1998-01-01 DOI: 10.1163/005225998X00028
D. Sturhan, M. S. Jairajpuri, W. Ahmad
Loofilaimus, a remarkable new genus of dorylaim nematodes is described from Iran. It is characterized by a continuous lip region with amalgamated lips; sclerotized cheilostome forming a bowl-shaped cavity, odontostyle short and wide, oesophagus conspicuously muscular along its entire length, amphidelphic gonads and short hemispheroid tails in both sexes. A new Family Loofilaimidae is proposed under Superfamily Tylencholaimoidea to accommodate this genus which has a curious mixture of characters of different dorylaim groups.
在伊朗描述了一种引人注目的线虫新属Loofilaimus。它的特点是连续的唇区与合并的嘴唇;骨口硬化形成碗状腔,齿状柱短而宽,食道沿其整个长度明显肌肉发达,两性两性性腺和短半球形尾巴。为了容纳这一奇特地混合了不同桃蚜类群特征的属,在桃蚜总科下提出了一个新的桃蚜科。
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引用次数: 3
The viability of Meloidogyne incognita eggs released from egg masses of different ages using different concentrations of sodium hypochlorite 不同浓度的次氯酸钠对不同年龄的卵群释放出的隐绒圆母卵的存活率的影响
Pub Date : 1998-01-01 DOI: 10.1163/005325998X00081
M. Ehwaeti, M. Phillips, D. Trudgill
The proportion of eggs of Meloidogyne incognita which hatched following extraction from egg masses with sodium hypochlorite solution (NaOCl) was shown to be related to the proportion that were embryonated at the time of extraction. Following NaOCl extraction, young egg masses (4-5 weeks after inoculation), which contained proportionally fewer embryonated eggs, yielded proportionally fewer juveniles than did old egg-masses (8-9 weeks). Using eggs extracted with NaOCl as inoculum a similar difference was observed in the numbers of juveniles which invaded tomato roots. Differences between two experiments in the rate at which eggs were released by the same nominal concentration of NaOCl were subsequently attributed to differences in chlorine (Cl) content. A further experiment where the Cl content was assayed showed that the numbers of eggs extracted increased, but that their viability decreased, with increasing concentration and duration of exposure to NaOCl. Overall, NaOCl was most effective when used on older egg masses with a high proportion of embryonated eggs.
用次氯酸钠溶液(NaOCl)从卵团中提取后,发现暗地长尾丝虫卵的孵化率与提取时的成胚率有关。NaOCl提取后,接种后4-5周的幼卵比接种后8-9周的老卵产生的幼卵数量要少。以NaOCl为接种剂提取的番茄卵,侵染番茄根部的幼虫数量也有类似的差异。两个实验在相同标称浓度的NaOCl下鸡蛋释放率的差异随后归因于氯(Cl)含量的差异。进一步测定氯含量的实验表明,随着NaOCl浓度的增加和暴露时间的延长,提取的卵数量增加,但卵活力下降。总的来说,NaOCl对胚胎比例高的老卵群最有效。
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引用次数: 8
期刊
Nematologica
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