Pub Date : 2024-08-12DOI: 10.1038/s41564-024-01769-9
Andreacarola Urso, Ian R. Monk, Ying-Tsun Cheng, Camilla Predella, Tania Wong Fok Lung, Erin M. Theiller, Jack Boylan, Sofya Perelman, Swikrity U. Baskota, Ahmed M. Moustafa, Gaurav Lohia, Ian A. Lewis, Benjamin P. Howden, Timothy P. Stinear, Nicolino V. Dorrello, Victor Torres, Alice S. Prince
Staphylococcus aureus is a pulmonary pathogen associated with substantial human morbidity and mortality. As vaccines targeting virulence determinants have failed to be protective in humans, other factors are likely involved in pathogenesis. Here we analysed transcriptomic responses of human clinical isolates of S. aureus from initial and chronic infections. We observed upregulated collagenase and proline transporter gene expression in chronic infection isolates. Metabolomics of bronchiolar lavage fluid and fibroblast infection, growth assays and analysis of bacterial mutant strains showed that airway fibroblasts produce collagen during S. aureus infection. Host-adapted bacteria upregulate collagenase, which degrades collagen and releases proline. S. aureus then imports proline, which fuels oxidative metabolism via the tricarboxylic acid cycle. Proline metabolism provides host-adapted S. aureus with a metabolic benefit enabling out-competition of non-adapted strains. These data suggest that clinical settings characterized by airway repair processes and fibrosis provide a milieu that promotes S. aureus adaptation and supports infection. Staphylococcus aureus upregulates collagenase and proline transporters to release collagen-derived proline and exploit fibroblasts as a nutrient source during chronic infection.
{"title":"Staphylococcus aureus adapts to exploit collagen-derived proline during chronic infection","authors":"Andreacarola Urso, Ian R. Monk, Ying-Tsun Cheng, Camilla Predella, Tania Wong Fok Lung, Erin M. Theiller, Jack Boylan, Sofya Perelman, Swikrity U. Baskota, Ahmed M. Moustafa, Gaurav Lohia, Ian A. Lewis, Benjamin P. Howden, Timothy P. Stinear, Nicolino V. Dorrello, Victor Torres, Alice S. Prince","doi":"10.1038/s41564-024-01769-9","DOIUrl":"10.1038/s41564-024-01769-9","url":null,"abstract":"Staphylococcus aureus is a pulmonary pathogen associated with substantial human morbidity and mortality. As vaccines targeting virulence determinants have failed to be protective in humans, other factors are likely involved in pathogenesis. Here we analysed transcriptomic responses of human clinical isolates of S. aureus from initial and chronic infections. We observed upregulated collagenase and proline transporter gene expression in chronic infection isolates. Metabolomics of bronchiolar lavage fluid and fibroblast infection, growth assays and analysis of bacterial mutant strains showed that airway fibroblasts produce collagen during S. aureus infection. Host-adapted bacteria upregulate collagenase, which degrades collagen and releases proline. S. aureus then imports proline, which fuels oxidative metabolism via the tricarboxylic acid cycle. Proline metabolism provides host-adapted S. aureus with a metabolic benefit enabling out-competition of non-adapted strains. These data suggest that clinical settings characterized by airway repair processes and fibrosis provide a milieu that promotes S. aureus adaptation and supports infection. Staphylococcus aureus upregulates collagenase and proline transporters to release collagen-derived proline and exploit fibroblasts as a nutrient source during chronic infection.","PeriodicalId":18992,"journal":{"name":"Nature Microbiology","volume":"9 10","pages":"2506-2521"},"PeriodicalIF":20.5,"publicationDate":"2024-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.nature.com/articles/s41564-024-01769-9.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141918777","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-08-08DOI: 10.1038/s41564-024-01798-4
Benjamin O Torres Salazar, Taulant Dema, Nadine A Schilling, Daniela Janek, Jan Bornikoel, Anne Berscheid, Ahmed M A Elsherbini, Sophia Krauss, Simon J Jaag, Michael Lämmerhofer, Min Li, Norah Alqahtani, Malcolm J Horsburgh, Tilmann Weber, José Manuel Beltrán-Beleña, Heike Brötz-Oesterhelt, Stephanie Grond, Bernhard Krismer, Andreas Peschel
{"title":"Author Correction: Commensal production of a broad-spectrum and short-lived antimicrobial peptide polyene eliminates nasal Staphylococcus aureus.","authors":"Benjamin O Torres Salazar, Taulant Dema, Nadine A Schilling, Daniela Janek, Jan Bornikoel, Anne Berscheid, Ahmed M A Elsherbini, Sophia Krauss, Simon J Jaag, Michael Lämmerhofer, Min Li, Norah Alqahtani, Malcolm J Horsburgh, Tilmann Weber, José Manuel Beltrán-Beleña, Heike Brötz-Oesterhelt, Stephanie Grond, Bernhard Krismer, Andreas Peschel","doi":"10.1038/s41564-024-01798-4","DOIUrl":"https://doi.org/10.1038/s41564-024-01798-4","url":null,"abstract":"","PeriodicalId":18992,"journal":{"name":"Nature Microbiology","volume":" ","pages":""},"PeriodicalIF":20.5,"publicationDate":"2024-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141907149","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-08-06DOI: 10.1038/s41564-024-01785-9
This month’s issue of Nature Microbiology has a focus on microbial ecology, showcasing how these dynamics might be harnessed to better understand and safeguard life on Earth.
{"title":"Bringing microbial ecology into focus","authors":"","doi":"10.1038/s41564-024-01785-9","DOIUrl":"10.1038/s41564-024-01785-9","url":null,"abstract":"This month’s issue of Nature Microbiology has a focus on microbial ecology, showcasing how these dynamics might be harnessed to better understand and safeguard life on Earth.","PeriodicalId":18992,"journal":{"name":"Nature Microbiology","volume":"9 8","pages":"1901-1902"},"PeriodicalIF":20.5,"publicationDate":"2024-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.nature.com/articles/s41564-024-01785-9.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141879060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-08-06DOI: 10.1038/s41564-024-01764-0
Daniel Sher, Daniel Segrè, Michael J. Follows
Metabolism is the complex network of chemical reactions occurring within every cell and organism, maintaining life, mediating ecosystem processes and affecting Earth’s climate. Experiments and models of microbial metabolism often focus on one specific scale, overlooking the connectivity between molecules, cells and ecosystems. Here we highlight quantitative metabolic principles that exhibit commonalities across scales, which we argue could help to achieve an integrated perspective on microbial life. Mass, electron and energy balance provide quantitative constraints on their flow within metabolic networks, organisms and ecosystems, shaping how each responds to its environment. The mechanisms underlying these flows, such as enzyme–substrate interactions, often involve encounter and handling stages that are represented by equations similar to those for cells and resources, or predators and prey. We propose that these formal similarities reflect shared principles and discuss how their investigation through experiments and models may contribute to a common language for studying microbial metabolism across scales. Mass, electron and energy balances define metabolic networks in a cell, but this framework could also be applied to interactions, ecosystems and global processes, creating a common language for microbial metabolism across scales.
{"title":"Quantitative principles of microbial metabolism shared across scales","authors":"Daniel Sher, Daniel Segrè, Michael J. Follows","doi":"10.1038/s41564-024-01764-0","DOIUrl":"10.1038/s41564-024-01764-0","url":null,"abstract":"Metabolism is the complex network of chemical reactions occurring within every cell and organism, maintaining life, mediating ecosystem processes and affecting Earth’s climate. Experiments and models of microbial metabolism often focus on one specific scale, overlooking the connectivity between molecules, cells and ecosystems. Here we highlight quantitative metabolic principles that exhibit commonalities across scales, which we argue could help to achieve an integrated perspective on microbial life. Mass, electron and energy balance provide quantitative constraints on their flow within metabolic networks, organisms and ecosystems, shaping how each responds to its environment. The mechanisms underlying these flows, such as enzyme–substrate interactions, often involve encounter and handling stages that are represented by equations similar to those for cells and resources, or predators and prey. We propose that these formal similarities reflect shared principles and discuss how their investigation through experiments and models may contribute to a common language for studying microbial metabolism across scales. Mass, electron and energy balances define metabolic networks in a cell, but this framework could also be applied to interactions, ecosystems and global processes, creating a common language for microbial metabolism across scales.","PeriodicalId":18992,"journal":{"name":"Nature Microbiology","volume":"9 8","pages":"1940-1953"},"PeriodicalIF":20.5,"publicationDate":"2024-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141895191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-08-06DOI: 10.1038/s41564-024-01749-z
Viviane Cordovez, Víctor J. Carrión, Gonzalo Rivas Torres, Diego A. Ortiz, Wilson Cabrera, Haig Balian, Adriana Karina Vivanco, Juan E. Pérez-Jaramillo, Jaime Chaves, Diana A. Pazmiño, Pieter van ’t Hof, Jos M. Raaijmakers
{"title":"Darwin’s expedition revisited to reveal the evolution of plant–microbe interactions on Galápagos","authors":"Viviane Cordovez, Víctor J. Carrión, Gonzalo Rivas Torres, Diego A. Ortiz, Wilson Cabrera, Haig Balian, Adriana Karina Vivanco, Juan E. Pérez-Jaramillo, Jaime Chaves, Diana A. Pazmiño, Pieter van ’t Hof, Jos M. Raaijmakers","doi":"10.1038/s41564-024-01749-z","DOIUrl":"10.1038/s41564-024-01749-z","url":null,"abstract":"","PeriodicalId":18992,"journal":{"name":"Nature Microbiology","volume":"9 8","pages":"1903-1905"},"PeriodicalIF":20.5,"publicationDate":"2024-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141895189","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Resident microbiota produces small molecules that influence the chemical microenvironments on leaves, but its signalling roles in pathogen defence are not yet well understood. Here we show that Aspergillus cvjetkovicii, enriched in rice leaf microbiota, subverts Rhizoctonia solani infections via small-molecule-mediated interspecies signalling. 2,4-Di-tert-butylphenol (2,4-DTBP), identified as a key signalling molecule within the Aspergillus-enriched microbiota, effectively neutralizes reactive oxygen species-dependent pathogenicity by switching off bZIP-activated AMT1 transcription in R. solani. Exogenous application of A. cvjetkovicii and 2,4-DTBP demonstrated varying degrees of protective effects against R. solani infection in diverse crops, including cucumber, maize, soybean and tomato. In rice field experiments, they reduced the R. solani-caused disease index to 19.7–32.2%, compared with 67.2–82.6% in the control group. Moreover, 2,4-DTBP showed activity against other rice phytopathogens, such as Fusarium fujikuroi. These findings reveal a defensive strategy against phytopathogens in the phyllosphere, highlighting the potential of symbiotic microbiota-driven neutralization of pathogenicity. Beneficial Aspergillus cvjetkovicii protects host plants against fungal diseases by inactivating pathogenicity-related gene transcription of phytopathogens via 2,4-DTBP signalling.
常驻微生物群产生的小分子可影响叶片上的化学微环境，但其在病原体防御中的信号作用尚未得到充分了解。在这里，我们展示了水稻叶片微生物群中富集的曲霉 cvjetkovicii 通过小分子介导的种间信号转导来颠覆根瘤菌的感染。2,4-二叔丁基苯酚（2,4-DTBP）被确定为曲霉富集微生物群中的一种关键信号分子，它通过关闭 bZIP 激活的 R. solani AMT1 转录，有效地中和了活性氧依赖的致病性。外源施用 A. cvjetkovicii 和 2,4-DTBP 对不同作物（包括黄瓜、玉米、大豆和番茄）的 R. solani 感染具有不同程度的保护作用。在水稻田间试验中，它们将 R. solani 引起的病害指数降低到 19.7-32.2%，而对照组为 67.2-82.6%。此外，2,4-DTBP 对其他水稻植物病原菌，如镰刀菌也有活性。这些发现揭示了植物叶球对植物病原体的防御策略，突出了共生微生物群驱动的中和致病性的潜力。
{"title":"Aspergillus cvjetkovicii protects against phytopathogens through interspecies chemical signalling in the phyllosphere","authors":"Xiaoyan Fan, Haruna Matsumoto, Haorong Xu, Hongda Fang, Qianqian Pan, Tianxing Lv, Chengfang Zhan, Xiaoxiao Feng, Xiaoyu Liu, Danrui Su, Mengyuan Fan, Zhonghua Ma, Gabriele Berg, Shaojia Li, Tomislav Cernava, Mengcen Wang","doi":"10.1038/s41564-024-01781-z","DOIUrl":"10.1038/s41564-024-01781-z","url":null,"abstract":"Resident microbiota produces small molecules that influence the chemical microenvironments on leaves, but its signalling roles in pathogen defence are not yet well understood. Here we show that Aspergillus cvjetkovicii, enriched in rice leaf microbiota, subverts Rhizoctonia solani infections via small-molecule-mediated interspecies signalling. 2,4-Di-tert-butylphenol (2,4-DTBP), identified as a key signalling molecule within the Aspergillus-enriched microbiota, effectively neutralizes reactive oxygen species-dependent pathogenicity by switching off bZIP-activated AMT1 transcription in R. solani. Exogenous application of A. cvjetkovicii and 2,4-DTBP demonstrated varying degrees of protective effects against R. solani infection in diverse crops, including cucumber, maize, soybean and tomato. In rice field experiments, they reduced the R. solani-caused disease index to 19.7–32.2%, compared with 67.2–82.6% in the control group. Moreover, 2,4-DTBP showed activity against other rice phytopathogens, such as Fusarium fujikuroi. These findings reveal a defensive strategy against phytopathogens in the phyllosphere, highlighting the potential of symbiotic microbiota-driven neutralization of pathogenicity. Beneficial Aspergillus cvjetkovicii protects host plants against fungal diseases by inactivating pathogenicity-related gene transcription of phytopathogens via 2,4-DTBP signalling.","PeriodicalId":18992,"journal":{"name":"Nature Microbiology","volume":"9 11","pages":"2862-2876"},"PeriodicalIF":20.5,"publicationDate":"2024-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141891856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-08-05DOI: 10.1038/s41564-024-01774-y
Mobile genetic element fluorescence in situ hybridization (MGE-FISH) creates spatial maps of target genes in microbiomes. We combine MGE-FISH with high phylogenetic resolution FISH (HiPR-FISH) to simultaneously map bacterial taxa and mobile genetic elements such as plasmids and phage, identifying their host taxa and revealing mobile genetic element spatial distribution.
{"title":"Microscopy methods map mobile genetic elements and their bacterial hosts","authors":"","doi":"10.1038/s41564-024-01774-y","DOIUrl":"10.1038/s41564-024-01774-y","url":null,"abstract":"Mobile genetic element fluorescence in situ hybridization (MGE-FISH) creates spatial maps of target genes in microbiomes. We combine MGE-FISH with high phylogenetic resolution FISH (HiPR-FISH) to simultaneously map bacterial taxa and mobile genetic elements such as plasmids and phage, identifying their host taxa and revealing mobile genetic element spatial distribution.","PeriodicalId":18992,"journal":{"name":"Nature Microbiology","volume":"9 9","pages":"2214-2215"},"PeriodicalIF":20.5,"publicationDate":"2024-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141891855","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-08-05DOI: 10.1038/s41564-024-01760-4
Jason Ziveri, Loïc Le Guennec, Isabel dos Santos Souza, Jean-Philipe Barnier, Samuel M. Walter, Youssouf Diallo, Yasmine Smail, Elodie Le Seac’h, Haniaa Bouzinba-Segard, Camille Faure, Philippe C. Morand, Irié Carel, Nicolas Perriere, Taliah Schmitt, Brigitte Izac, Franck Letourneur, Mathieu Coureuil, Thomas Rattei, Xavier Nassif, Sandrine Bourdoulous
Loss of endothelial integrity and vascular leakage are central features of sepsis pathogenesis; however, no effective therapeutic mechanisms for preserving endothelial integrity are available. Here we show that, compared to dermal microvessels, brain microvessels resist infection by Neisseria meningitidis, a bacterial pathogen that causes sepsis and meningitis. By comparing the transcriptional responses to infection in dermal and brain endothelial cells, we identified angiopoietin-like 4 as a key factor produced by the brain endothelium that preserves blood–brain barrier integrity during bacterial sepsis. Conversely, angiopoietin-like 4 is produced at lower levels in the peripheral endothelium. Treatment with recombinant angiopoietin-like 4 reduced vascular leakage, organ failure and death in mouse models of lethal sepsis and N. meningitidis infection. Protection was conferred by a previously uncharacterized domain of angiopoietin-like 4, through binding to the heparan proteoglycan, syndecan-4. These findings reveal a potential strategy to prevent endothelial dysfunction and improve outcomes in patients with sepsis. Therapeutic administration of angiopoietin-like 4 prevents shock during Neisseria meningitidis infection or lipopolysaccharide-induced sepsis in mice.
{"title":"Angiopoietin-like 4 protects against endothelial dysfunction during bacterial sepsis","authors":"Jason Ziveri, Loïc Le Guennec, Isabel dos Santos Souza, Jean-Philipe Barnier, Samuel M. Walter, Youssouf Diallo, Yasmine Smail, Elodie Le Seac’h, Haniaa Bouzinba-Segard, Camille Faure, Philippe C. Morand, Irié Carel, Nicolas Perriere, Taliah Schmitt, Brigitte Izac, Franck Letourneur, Mathieu Coureuil, Thomas Rattei, Xavier Nassif, Sandrine Bourdoulous","doi":"10.1038/s41564-024-01760-4","DOIUrl":"10.1038/s41564-024-01760-4","url":null,"abstract":"Loss of endothelial integrity and vascular leakage are central features of sepsis pathogenesis; however, no effective therapeutic mechanisms for preserving endothelial integrity are available. Here we show that, compared to dermal microvessels, brain microvessels resist infection by Neisseria meningitidis, a bacterial pathogen that causes sepsis and meningitis. By comparing the transcriptional responses to infection in dermal and brain endothelial cells, we identified angiopoietin-like 4 as a key factor produced by the brain endothelium that preserves blood–brain barrier integrity during bacterial sepsis. Conversely, angiopoietin-like 4 is produced at lower levels in the peripheral endothelium. Treatment with recombinant angiopoietin-like 4 reduced vascular leakage, organ failure and death in mouse models of lethal sepsis and N. meningitidis infection. Protection was conferred by a previously uncharacterized domain of angiopoietin-like 4, through binding to the heparan proteoglycan, syndecan-4. These findings reveal a potential strategy to prevent endothelial dysfunction and improve outcomes in patients with sepsis. Therapeutic administration of angiopoietin-like 4 prevents shock during Neisseria meningitidis infection or lipopolysaccharide-induced sepsis in mice.","PeriodicalId":18992,"journal":{"name":"Nature Microbiology","volume":"9 9","pages":"2434-2447"},"PeriodicalIF":20.5,"publicationDate":"2024-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141891857","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-08-02DOI: 10.1038/s41564-024-01745-3
Madeleine J. H. van Oppen
Madeleine van Oppen describes her research on the family Symbiodiniaceae, the microalgae that could be instrumental in safeguarding coral reefs against the effects of climate change.
Madeleine van Oppen 介绍了她对共生藻科（Symbiodiniaceae）的研究，这种微型藻类可能有助于保护珊瑚礁免受气候变化的影响。
{"title":"Shining light on the coral photosymbiont family Symbiodiniaceae","authors":"Madeleine J. H. van Oppen","doi":"10.1038/s41564-024-01745-3","DOIUrl":"10.1038/s41564-024-01745-3","url":null,"abstract":"Madeleine van Oppen describes her research on the family Symbiodiniaceae, the microalgae that could be instrumental in safeguarding coral reefs against the effects of climate change.","PeriodicalId":18992,"journal":{"name":"Nature Microbiology","volume":"9 8","pages":"1909-1910"},"PeriodicalIF":20.5,"publicationDate":"2024-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141879063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-08-02DOI: 10.1038/s41564-024-01770-2
Simone Pecetta, Rino Rappuoli
A genetic approach based on the introduction of premature termination codons can attenuate SARS-CoV-2 and induce protective mucosal immunity.
基于引入过早终止密码子的基因方法可以减弱 SARS-CoV-2 并诱导保护性粘膜免疫。
{"title":"The evolving landscape of live attenuated COVID-19 vaccines","authors":"Simone Pecetta, Rino Rappuoli","doi":"10.1038/s41564-024-01770-2","DOIUrl":"10.1038/s41564-024-01770-2","url":null,"abstract":"A genetic approach based on the introduction of premature termination codons can attenuate SARS-CoV-2 and induce protective mucosal immunity.","PeriodicalId":18992,"journal":{"name":"Nature Microbiology","volume":"9 8","pages":"1911-1913"},"PeriodicalIF":20.5,"publicationDate":"2024-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141879065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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