Pub Date : 2022-09-01DOI: 10.17116/molgen20224003141
L. V. Bezuglova, L. Osipova, E. Sergeeva, I. Deliy, L. Tabikhanova, S. Netesov, I. Netesova
Hepatitis B is a major public health problem worldwide. The indigenous population of remote regions with harsh living conditions, such as the Far North of Russia, with possibly unique genetic mutations in the local strains of this pathogen, remains poorly studied. The aim of the study was to determine the prevalence of serological HBV markers in blood–plasma samples of the population of the Far North of Russia and to determine HBV genotypes and HBsAg subtypes in blood–plasma samples containing HBsAg. We studied 702 blood–plasma samples taken from the indigenous population (Tundra Nenets) of the village of Gyda in the Tazovsky district of the Yamalo-Nenets autonomous okrug (YaNAO) (north of the Gydan Peninsula). Detection of serological HBV markers—HBsAg, anti-HBs, and anti-Hbcore—was carried out using JSC Vector-Best reagent kits. HBV genotypes and HBsAg subtypes in the samples were determined according to our own original procedure with monoclonal antibodies and by classical molecular-biological methods. The prevalence of HBsAg in the entire sample was 0.4% (3/702). For the subgroup of samples (persons older than 15 years during the first two expeditions, 300 samples), an extended analysis of the markers was carried out. The following frequencies were determined for them: HBsAg, 0.7% (2/300); anti-HBs, 63.7% (191/300); and anti-HBcore, 11.3% (34/300). HBV statuses were established for this subgroup: the presence of HBV infection (0.7%), past hepatitis B infection (9.3%), and immunity as result of vaccination (54.3%). Genotype D was established for two samples containing HBsAg (subtypes ayw2 and ayw3); the results of the two used methods coincided. The prevalence of HBsAg in the Tazovsky and adjacent Purovsky districts (the main population being Nenets, previously published data) differed (0.4% vs. 1.7%, p < 0.05), presumably due to vaccination (samples of the Purovsky district were collected almost 20 years earlier), as well as the distribution of HBV genotypes. The Tazovsky district of YaNAO is now a low endemic region in terms of HBV infection. The HBV isolates belong to the most common HBV genotype D in the Russian Federation (HBsAg subtypes ayw2, ayw3).
乙型肝炎是世界范围内的一个主要公共卫生问题。生活条件恶劣的偏远地区的土著居民，如俄罗斯的远北地区，可能在这种病原体的当地菌株中有独特的基因突变，但对这些地区的研究仍然很少。该研究的目的是确定俄罗斯远北地区人群血浆样本中血清学HBV标志物的流行情况，并确定含HBsAg的血浆样本中HBV基因型和HBsAg亚型。我们研究了采自亚马尔-涅涅茨自治区(YaNAO)(格丹半岛北部)Tazovsky区Gyda村土著居民(苔原涅涅茨人)的702份血浆样本。采用JSC Vector-Best试剂盒检测血清HBV标志物hbsag、anti- hbbs和anti- hbcore。样品中的HBV基因型和HBsAg亚型根据我们自己的原始程序用单克隆抗体和经典的分子生物学方法测定。HBsAg阳性率为0.4%(3/702)。对于样本亚组(前两次探险中年龄超过15岁的人，300个样本)，对标记进行了扩展分析。他们的频率测定如下:HBsAg, 0.7% (2/300);anti-HBs, 63.7% (191/300);anti-HBcore为11.3%(34/300)。确定该亚组的HBV状态:存在HBV感染(0.7%)，既往乙型肝炎感染(9.3%)，接种疫苗后免疫(54.3%)。两份含HBsAg样本(ayw2和ayw3亚型)建立基因型D;两种方法的结果是一致的。Tazovsky和邻近的Purovsky地区(主要人群为涅涅茨人，先前公布的数据)的HBsAg患病率不同(0.4% vs. 1.7%， p < 0.05)，可能是由于疫苗接种(Purovsky地区的样本是在近20年前收集的)以及HBV基因型的分布。YaNAO的Tazovsky区现在是HBV感染的低流行区。HBV分离株属于俄罗斯联邦最常见的HBV基因型D (HBsAg亚型ayw2, ayw3)。
{"title":"Markers of Viral Hepatitis B in Blood–Plasma Samples of the Indigenous Population of the Far North of Russia. HBV Genotypes and HBsAg Subtypes","authors":"L. V. Bezuglova, L. Osipova, E. Sergeeva, I. Deliy, L. Tabikhanova, S. Netesov, I. Netesova","doi":"10.17116/molgen20224003141","DOIUrl":"https://doi.org/10.17116/molgen20224003141","url":null,"abstract":"Hepatitis B is a major public health problem worldwide. The indigenous population of remote regions with harsh living conditions, such as the Far North of Russia, with possibly unique genetic mutations in the local strains of this pathogen, remains poorly studied. The aim of the study was to determine the prevalence of serological HBV markers in blood–plasma samples of the population of the Far North of Russia and to determine HBV genotypes and HBsAg subtypes in blood–plasma samples containing HBsAg. We studied 702 blood–plasma samples taken from the indigenous population (Tundra Nenets) of the village of Gyda in the Tazovsky district of the Yamalo-Nenets autonomous okrug (YaNAO) (north of the Gydan Peninsula). Detection of serological HBV markers—HBsAg, anti-HBs, and anti-Hbcore—was carried out using JSC Vector-Best reagent kits. HBV genotypes and HBsAg subtypes in the samples were determined according to our own original procedure with monoclonal antibodies and by classical molecular-biological methods. The prevalence of HBsAg in the entire sample was 0.4% (3/702). For the subgroup of samples (persons older than 15 years during the first two expeditions, 300 samples), an extended analysis of the markers was carried out. The following frequencies were determined for them: HBsAg, 0.7% (2/300); anti-HBs, 63.7% (191/300); and anti-HBcore, 11.3% (34/300). HBV statuses were established for this subgroup: the presence of HBV infection (0.7%), past hepatitis B infection (9.3%), and immunity as result of vaccination (54.3%). Genotype D was established for two samples containing HBsAg (subtypes ayw2 and ayw3); the results of the two used methods coincided. The prevalence of HBsAg in the Tazovsky and adjacent Purovsky districts (the main population being Nenets, previously published data) differed (0.4% vs. 1.7%, p < 0.05), presumably due to vaccination (samples of the Purovsky district were collected almost 20 years earlier), as well as the distribution of HBV genotypes. The Tazovsky district of YaNAO is now a low endemic region in terms of HBV infection. The HBV isolates belong to the most common HBV genotype D in the Russian Federation (HBsAg subtypes ayw2, ayw3).","PeriodicalId":19005,"journal":{"name":"Molecular Genetics, Microbiology and Virology","volume":"2 1","pages":"146-152"},"PeriodicalIF":0.5,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76202426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-09-01DOI: 10.17116/molgen20224003132
T. V. Solomay, M. Malakhova, E. Shitikov, D. Bespiatykh, V. Veselovsky, T. A. Semenenko, D. Smirnova, A. Gracheva, E. B. Fayzuloev
Abstract Approximately 90% of the world’s population is infected with the Epstein–Barr virus (EBV). Damage to health and high economical costs require the development of vaccines against EBV. The variability of individual genes may affect the success of the vaccination campaign and will necessitate dynamic update of antigenic composition of vaccines. The aim of this study was to assess the variability of EBV gp350 and EBNA2 genes isolated from saliva of dental clinic personnel in Moscow oblast. Biosamples were obtained from 105 employees at four dental clinics. For EBV DNA-positive samples, the EBNA2 and gp350 genes were amplified using nested PCR. The nucleotide sequence of gp350 gene was determined during sequencing. A maximum likelihood phylogenetic tree was constructed from the N-terminal fragment of gp350 protein, using B95-8 strain as a reference genome. Phylogenetic analysis also included 222 EBV samples from the NCBI database. The EBNA2 genotype and the whole gp350 gene sequence were determined for 31 DNA samples. Based on the phylogenetic analysis of gp350 protein, the Russian virus population was uniformly distributed along the tree. Meanwhile, 30 samples fell into the Aa clade (A, genotype A for EBNA2 gene; a, similarity of the gp350 sequence with B95-8 [NC_007605.1]) and one sample belonged to the Bb clade (B, genotype B for EBNA2 ; b , similarity of gp350 with Jijoye [LN827800.1]). For 30 Russian samples of Aa genotype, 22 individual profiles and 16 unique mutations were found. Identical gp350 profiles were found amongst staff working in close professional contact. The identified features indicated the need for further phylogenetic studies of samples collected in Russia to develop and introduce vaccines and monitor their efficiency.
{"title":"Epstein–Barr Virus: Evaluation of gp350 and EBNA2 Gene Variability","authors":"T. V. Solomay, M. Malakhova, E. Shitikov, D. Bespiatykh, V. Veselovsky, T. A. Semenenko, D. Smirnova, A. Gracheva, E. B. Fayzuloev","doi":"10.17116/molgen20224003132","DOIUrl":"https://doi.org/10.17116/molgen20224003132","url":null,"abstract":"Abstract Approximately 90% of the world’s population is infected with the Epstein–Barr virus (EBV). Damage to health and high economical costs require the development of vaccines against EBV. The variability of individual genes may affect the success of the vaccination campaign and will necessitate dynamic update of antigenic composition of vaccines. The aim of this study was to assess the variability of EBV gp350 and EBNA2 genes isolated from saliva of dental clinic personnel in Moscow oblast. Biosamples were obtained from 105 employees at four dental clinics. For EBV DNA-positive samples, the EBNA2 and gp350 genes were amplified using nested PCR. The nucleotide sequence of gp350 gene was determined during sequencing. A maximum likelihood phylogenetic tree was constructed from the N-terminal fragment of gp350 protein, using B95-8 strain as a reference genome. Phylogenetic analysis also included 222 EBV samples from the NCBI database. The EBNA2 genotype and the whole gp350 gene sequence were determined for 31 DNA samples. Based on the phylogenetic analysis of gp350 protein, the Russian virus population was uniformly distributed along the tree. Meanwhile, 30 samples fell into the Aa clade (A, genotype A for EBNA2 gene; a, similarity of the gp350 sequence with B95-8 [NC_007605.1]) and one sample belonged to the Bb clade (B, genotype B for EBNA2 ; b , similarity of gp350 with Jijoye [LN827800.1]). For 30 Russian samples of Aa genotype, 22 individual profiles and 16 unique mutations were found. Identical gp350 profiles were found amongst staff working in close professional contact. The identified features indicated the need for further phylogenetic studies of samples collected in Russia to develop and introduce vaccines and monitor their efficiency.","PeriodicalId":19005,"journal":{"name":"Molecular Genetics, Microbiology and Virology","volume":"36 1","pages":"138-145"},"PeriodicalIF":0.5,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79381847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-09-01DOI: 10.3103/s0891416822030090
A. Vyazovaya, O. Narvskaya, V. Vedernikov, M. A. Nikolskiy
{"title":"Single Nucleotide Polymorphisms in the U86 Gene of Human betaherpesvirus 6B","authors":"A. Vyazovaya, O. Narvskaya, V. Vedernikov, M. A. Nikolskiy","doi":"10.3103/s0891416822030090","DOIUrl":"https://doi.org/10.3103/s0891416822030090","url":null,"abstract":"","PeriodicalId":19005,"journal":{"name":"Molecular Genetics, Microbiology and Virology","volume":"23 1","pages":""},"PeriodicalIF":0.5,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87480096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-09-01DOI: 10.3103/S089141682203003X
Elmira Babakanrad, T. Mohammadian, D. Esmaeili, P. Behzadi
{"title":"Efficacy of the Apoptotic Activity of CpsA-CpsC-L-ACAN Fusion Peptide against HeLa Cell Line","authors":"Elmira Babakanrad, T. Mohammadian, D. Esmaeili, P. Behzadi","doi":"10.3103/S089141682203003X","DOIUrl":"https://doi.org/10.3103/S089141682203003X","url":null,"abstract":"","PeriodicalId":19005,"journal":{"name":"Molecular Genetics, Microbiology and Virology","volume":"89 1","pages":"153-158"},"PeriodicalIF":0.5,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72861533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-09-01DOI: 10.3103/s0891416822030065
T. Ilyina
{"title":"Adaptive Immunity Systems of Bacteria: Association with Self-Synthesizing Transposons, Polyfunctionality","authors":"T. Ilyina","doi":"10.3103/s0891416822030065","DOIUrl":"https://doi.org/10.3103/s0891416822030065","url":null,"abstract":"","PeriodicalId":19005,"journal":{"name":"Molecular Genetics, Microbiology and Virology","volume":"33 1","pages":""},"PeriodicalIF":0.5,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80513597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-06-01DOI: 10.3103/S0891416822020070
A. Vyazovaya, N. Gavrilova, A. Gerasimova, A. Bychkova, I. Avadenii, E. Anikieva, N. Solovieva, V. Zhuravlev, I. Mokrousov, O. Narvskaya
{"title":"Molecular-Genetic Monitoring of the Mycobacterium tuberculosis Population in Murmansk Oblast","authors":"A. Vyazovaya, N. Gavrilova, A. Gerasimova, A. Bychkova, I. Avadenii, E. Anikieva, N. Solovieva, V. Zhuravlev, I. Mokrousov, O. Narvskaya","doi":"10.3103/S0891416822020070","DOIUrl":"https://doi.org/10.3103/S0891416822020070","url":null,"abstract":"","PeriodicalId":19005,"journal":{"name":"Molecular Genetics, Microbiology and Virology","volume":"81 1","pages":"71 - 77"},"PeriodicalIF":0.5,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76220342","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-06-01DOI: 10.3103/S0891416822020069
N. Smirnova, D. A. Rybal’chenko, E. Shchelkanova, Yu. V. Lozovsky, Ya. M. Krasnov, V. Kutyrev
{"title":"Variability of Multiple Resistance to Antibiotics in a Cholera Agent Associated with Different Types of SXT Element and Spontaneous Chromosome Mutations","authors":"N. Smirnova, D. A. Rybal’chenko, E. Shchelkanova, Yu. V. Lozovsky, Ya. M. Krasnov, V. Kutyrev","doi":"10.3103/S0891416822020069","DOIUrl":"https://doi.org/10.3103/S0891416822020069","url":null,"abstract":"","PeriodicalId":19005,"journal":{"name":"Molecular Genetics, Microbiology and Virology","volume":"100 1","pages":"78-85"},"PeriodicalIF":0.5,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79435428","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-06-01DOI: 10.3103/S0891416822020057
M. Rastegar Shariat Panahi, M. Mirzaei, H. Tebyaniyan, S. Khodi, M. Heiat, A. Latifi
{"title":"The Investigation of Clone and Expression of Butyrylcholinesterase in Pichia pastoris for Parathion Hydrolysis","authors":"M. Rastegar Shariat Panahi, M. Mirzaei, H. Tebyaniyan, S. Khodi, M. Heiat, A. Latifi","doi":"10.3103/S0891416822020057","DOIUrl":"https://doi.org/10.3103/S0891416822020057","url":null,"abstract":"","PeriodicalId":19005,"journal":{"name":"Molecular Genetics, Microbiology and Virology","volume":"44 1","pages":"99-106"},"PeriodicalIF":0.5,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87462552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-06-01DOI: 10.3103/S0891416822020045
S. Evstigneeva, D. Mokeev, L. Petrova, A. Shelud’ko
{"title":"Genetic Aspects of Mechanosensitivity in the Alphaproteobacteria Azospirillum baldaniorum with Mixed Flagellation","authors":"S. Evstigneeva, D. Mokeev, L. Petrova, A. Shelud’ko","doi":"10.3103/S0891416822020045","DOIUrl":"https://doi.org/10.3103/S0891416822020045","url":null,"abstract":"","PeriodicalId":19005,"journal":{"name":"Molecular Genetics, Microbiology and Virology","volume":"88 1","pages":"86-90"},"PeriodicalIF":0.5,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83817715","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-06-01DOI: 10.3103/S0891416822020033
L. V. Bezuglova, O. Isaeva, A. A. Karlsen, L. Ilchenko, S. S. Sleptsova, A. Saryglar, V. A. Poryvaeva, Ya. D. Mosina, O. A. Agafonova, A. K. Mogilnykh, K. Kyuregyan, M. Mikhailov, S. Netesov, I. Netesova
{"title":"Hepatitis B Virus Genotypes in Patients with Hepatitis D as Determined by the Panel of Their Own Development of Monoclonal Antibodies","authors":"L. V. Bezuglova, O. Isaeva, A. A. Karlsen, L. Ilchenko, S. S. Sleptsova, A. Saryglar, V. A. Poryvaeva, Ya. D. Mosina, O. A. Agafonova, A. K. Mogilnykh, K. Kyuregyan, M. Mikhailov, S. Netesov, I. Netesova","doi":"10.3103/S0891416822020033","DOIUrl":"https://doi.org/10.3103/S0891416822020033","url":null,"abstract":"","PeriodicalId":19005,"journal":{"name":"Molecular Genetics, Microbiology and Virology","volume":"22 1","pages":"91-98"},"PeriodicalIF":0.5,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74191869","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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