Pub Date : 2024-09-10DOI: 10.1177/1934578x241265934
Maria Giulia Manzione, Sara Vitalini, Farukh Sharopov, Xavier Capó, Marcello Iriti, Miquel Martorell, Raffaele Pezzani
Pinus mugo Turra is a coniferous species of shrub or small tree typical of central and south-eastern Europe. It is commonly called mugo pine, mountain pine, or dwarf mountain pine referring to its habit and small size. Indeed, this plant lives at higher altitude above 1400 m above the see level where it deals with harsh conditions typical of high mountain. From an ethnotraditional point of view, P. mugo is mainly used for respiratory disorders and wound healing. In particular, its essential oils have been shown to possess interesting antimicrobial and antioxidant activities, which can substantiate its potential therapeutic effect in pulmonary and urinary tract diseases, as well as antinflammatory and antitumor effects. This review also offers a summary on the chemical constituents of P. mugo that contribute to its therapeutic potential.
{"title":"Pinus mugo Turra and its Therapeutic Potential: A Narrative Review","authors":"Maria Giulia Manzione, Sara Vitalini, Farukh Sharopov, Xavier Capó, Marcello Iriti, Miquel Martorell, Raffaele Pezzani","doi":"10.1177/1934578x241265934","DOIUrl":"https://doi.org/10.1177/1934578x241265934","url":null,"abstract":"Pinus mugo Turra is a coniferous species of shrub or small tree typical of central and south-eastern Europe. It is commonly called mugo pine, mountain pine, or dwarf mountain pine referring to its habit and small size. Indeed, this plant lives at higher altitude above 1400 m above the see level where it deals with harsh conditions typical of high mountain. From an ethnotraditional point of view, P. mugo is mainly used for respiratory disorders and wound healing. In particular, its essential oils have been shown to possess interesting antimicrobial and antioxidant activities, which can substantiate its potential therapeutic effect in pulmonary and urinary tract diseases, as well as antinflammatory and antitumor effects. This review also offers a summary on the chemical constituents of P. mugo that contribute to its therapeutic potential.","PeriodicalId":19019,"journal":{"name":"Natural Product Communications","volume":"117 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142177255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-09DOI: 10.1177/1934578x241272485
Ali Aberoumand
Background: The presence of toxic metals in food can be a risk to food safety and public health. Methods: The Iranian canned tuna was analyzed for nickel level after wet digestion with acids using Graphite Furnace Atomic Absorption Spectrometry (GFAAS). Results: The average concentration of nickel in canned and dead fresh fish found 4.20 ppb and 1.64 ppb. A significant difference in nickel level was observed between canned and dead fresh fish samples in this study. Although the concentration of nickel per g in the fillet after processing was relatively low, but the total amount of nickel in the sample shown change (256.01%). The results in present study showed the protein content in the canned fish increased to 13.17%. The moisture content in the processed fish decreased to 8.80%. The fat content in the canned fish increased to 12.86%. Conclusion: Although the protein and moisture contents significantly increased and decreased respectively after processing, but none of these factors were related to the change in nickel concentration. It may be recommended to regularly monitor the concentration of nickel metal in aquatic food products, so that human health was not at risk.
{"title":"Effects of Processing on Nickel Content and its Relationship with Proximate Composition in dead fresh and canned tuna fish in Iran","authors":"Ali Aberoumand","doi":"10.1177/1934578x241272485","DOIUrl":"https://doi.org/10.1177/1934578x241272485","url":null,"abstract":"Background: The presence of toxic metals in food can be a risk to food safety and public health. Methods: The Iranian canned tuna was analyzed for nickel level after wet digestion with acids using Graphite Furnace Atomic Absorption Spectrometry (GFAAS). Results: The average concentration of nickel in canned and dead fresh fish found 4.20 ppb and 1.64 ppb. A significant difference in nickel level was observed between canned and dead fresh fish samples in this study. Although the concentration of nickel per g in the fillet after processing was relatively low, but the total amount of nickel in the sample shown change (256.01%). The results in present study showed the protein content in the canned fish increased to 13.17%. The moisture content in the processed fish decreased to 8.80%. The fat content in the canned fish increased to 12.86%. Conclusion: Although the protein and moisture contents significantly increased and decreased respectively after processing, but none of these factors were related to the change in nickel concentration. It may be recommended to regularly monitor the concentration of nickel metal in aquatic food products, so that human health was not at risk.","PeriodicalId":19019,"journal":{"name":"Natural Product Communications","volume":"3 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142177268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
BackgroundMacrophage-derived foam cells are essential in the progression of atherosclerosis (AS). Based on our previous study, the Huxin Formula (HXF), a traditional Chinese medicine formula, demonstrates potential in anti-atherosclerosis. Nevertheless, it is still unknown how HXF affects the formation of foam cells derived from THP-1 macrophages.PurposeThis research aims to examine the preventive role of HXF in the development of foam cells and its underlying molecular mechanism.MethodsTHP-1 derived macrophages and THP-1 cells overexpressing LOX-1 (LV-OLR1) were exposed to ox-LDL to establish foam cell models, and then treated with HXF. Meantime, Oil red O staining was used to detect lipid droplet production. ELISA kit was performed to measure intracellular levels of IL-6 and TGF-β. RT-qPCR and Western Blot were then utilized to determine the LOX-1 and NF-κB mRNA/protein levels.ResultsThe findings indicated that HXF treatment potently reduced the lipid accumulation, downregulated IL-6 levels and upregulated TGF-β levels. However, this impact was almost reversed when LOX-1 was overexpressed in THP-1 cells stimulated with ox-LDL. Moreover, THP-1 were treated with HXF markedly reduced the levels of LOX-1 and NF-κB mRNA/protein, whereas overexpressing LOX-1 significantly reversed this effect.ConclusionHXF reduced the formation of foam cell in ox-LDL-stimulated THP-1 macrophages via inhibiting lipid accumulation and inflammation through regulating the LOX-1/ NF-κB pathway. These present findings further indicate a potential beneficial role of HXF in ameliorating atherosclerosis and foam cell formation, while provide a novel potential therapeutic strategy for preventing atherosclerosis.
{"title":"Huxin Formula Inhibits Oxidized low-Density Lipoprotein-Induced Foam Cell Formation in THP-1 Macrophages via the LOX-1/NF-κB Pathway","authors":"Qiaohuang Zeng, Xiaomin Ou, Jing Cai, Taohua Lan, Weihui Lu, Wei Jiang","doi":"10.1177/1934578x241282836","DOIUrl":"https://doi.org/10.1177/1934578x241282836","url":null,"abstract":"BackgroundMacrophage-derived foam cells are essential in the progression of atherosclerosis (AS). Based on our previous study, the Huxin Formula (HXF), a traditional Chinese medicine formula, demonstrates potential in anti-atherosclerosis. Nevertheless, it is still unknown how HXF affects the formation of foam cells derived from THP-1 macrophages.PurposeThis research aims to examine the preventive role of HXF in the development of foam cells and its underlying molecular mechanism.MethodsTHP-1 derived macrophages and THP-1 cells overexpressing LOX-1 (LV-OLR1) were exposed to ox-LDL to establish foam cell models, and then treated with HXF. Meantime, Oil red O staining was used to detect lipid droplet production. ELISA kit was performed to measure intracellular levels of IL-6 and TGF-β. RT-qPCR and Western Blot were then utilized to determine the LOX-1 and NF-κB mRNA/protein levels.ResultsThe findings indicated that HXF treatment potently reduced the lipid accumulation, downregulated IL-6 levels and upregulated TGF-β levels. However, this impact was almost reversed when LOX-1 was overexpressed in THP-1 cells stimulated with ox-LDL. Moreover, THP-1 were treated with HXF markedly reduced the levels of LOX-1 and NF-κB mRNA/protein, whereas overexpressing LOX-1 significantly reversed this effect.ConclusionHXF reduced the formation of foam cell in ox-LDL-stimulated THP-1 macrophages via inhibiting lipid accumulation and inflammation through regulating the LOX-1/ NF-κB pathway. These present findings further indicate a potential beneficial role of HXF in ameliorating atherosclerosis and foam cell formation, while provide a novel potential therapeutic strategy for preventing atherosclerosis.","PeriodicalId":19019,"journal":{"name":"Natural Product Communications","volume":"15 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142177271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-07DOI: 10.1177/1934578x241281571
Wei Tian, Weiqian Zhou, Shi Huang, Qiuyun Qin, Weilong Fang, Lijie Zhou, Xihong Yang
ContextA prescription medication called Anchang Decoction (ACD) has anti-inflammatory properties.ObjectiveTo evaluate the mechanism of ACD against inflammation.Materials and methodsThe “pharmacodynamic constituents - potential targets” and “protein interaction networks” were mapped using Cytoscape software and the STRING database, respectively. The degree of binding between important pharmacodynamic components of ACD and possible targets was then examined using molecular docking analysis using Autodock Vina and PyMOL software, and GO and KEGG pathway enrichment analysis using the David database and the Weishengxin online tool. These findings were eventually confirmed in vitro.ResultsAfter the intersection of the two, 539 inflammatory targets and 217 related targets, 34 main active components, and 42 potential anti-inflammatory targets were obtained. These include AKT1, MAPK14, SRC, EGFR, GSK3B, MMP9, MMP2, PTGS2, SYK, ESR1, and MMP2 and GO enrichment results. These three key targets are chosen as downstream validation targets for experimental verification. Furthermore, the colonic tissue and mucosa of ACD group were undamaged in comparison to the model group, and there was no sign of inflammatory cell infiltration. According to CCK-8 data, treatment with 20% ACD drug-containing serum resulted in a significant increase in RAW264.7 cell viability (P < 0.05) when compared to the normal serum group; Serum of ACD-containing medication may considerably lower the NO content of macrophage inflammation and prevent the production of inflammatory markers like TNF-α and IL-6 (P < 0.05); The expression levels of AKT1, MAPK14, and SRC proteins were considerably reduced by ACD in RAW264.7 macrophage inflammation, according to Western Blot data (P < 0.05).Discussion and conclusionsACD exerts anti-inflammatory effects through multi-component interaction with the target, and the mechanism may involve the inhibition of the release of inflammatory cytokines by AKT, MAPK and non-receptor tyrosine kinase signaling pathways. Here, the molecular mechanism of ACD against inflammation was partially clarified and experimentally validated, offering theoretical evidence for more effective clinical application.
{"title":"Mechanism of Anchang Decoction in Treatment of Anti-Inflammatory Effect Based on Network Pharmacology, Molecular Docking and Experimental Verification","authors":"Wei Tian, Weiqian Zhou, Shi Huang, Qiuyun Qin, Weilong Fang, Lijie Zhou, Xihong Yang","doi":"10.1177/1934578x241281571","DOIUrl":"https://doi.org/10.1177/1934578x241281571","url":null,"abstract":"ContextA prescription medication called Anchang Decoction (ACD) has anti-inflammatory properties.ObjectiveTo evaluate the mechanism of ACD against inflammation.Materials and methodsThe “pharmacodynamic constituents - potential targets” and “protein interaction networks” were mapped using Cytoscape software and the STRING database, respectively. The degree of binding between important pharmacodynamic components of ACD and possible targets was then examined using molecular docking analysis using Autodock Vina and PyMOL software, and GO and KEGG pathway enrichment analysis using the David database and the Weishengxin online tool. These findings were eventually confirmed in vitro.ResultsAfter the intersection of the two, 539 inflammatory targets and 217 related targets, 34 main active components, and 42 potential anti-inflammatory targets were obtained. These include AKT1, MAPK14, SRC, EGFR, GSK3B, MMP9, MMP2, PTGS2, SYK, ESR1, and MMP2 and GO enrichment results. These three key targets are chosen as downstream validation targets for experimental verification. Furthermore, the colonic tissue and mucosa of ACD group were undamaged in comparison to the model group, and there was no sign of inflammatory cell infiltration. According to CCK-8 data, treatment with 20% ACD drug-containing serum resulted in a significant increase in RAW264.7 cell viability (P < 0.05) when compared to the normal serum group; Serum of ACD-containing medication may considerably lower the NO content of macrophage inflammation and prevent the production of inflammatory markers like TNF-α and IL-6 (P < 0.05); The expression levels of AKT1, MAPK14, and SRC proteins were considerably reduced by ACD in RAW264.7 macrophage inflammation, according to Western Blot data (P < 0.05).Discussion and conclusionsACD exerts anti-inflammatory effects through multi-component interaction with the target, and the mechanism may involve the inhibition of the release of inflammatory cytokines by AKT, MAPK and non-receptor tyrosine kinase signaling pathways. Here, the molecular mechanism of ACD against inflammation was partially clarified and experimentally validated, offering theoretical evidence for more effective clinical application.","PeriodicalId":19019,"journal":{"name":"Natural Product Communications","volume":"16 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142177272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objectives/background: The infectious consequences of microorganisms are varied due to resistance to existing antimicrobial drugs or new types of microbes causing lethal consequences for humans. Investigation are being performed to control different viral diseases; therefore; medicinal and herbal plants which have a variety of biological properties, can be evaluated for their anti-viral potential. Methods: The antiviral potential of NADES and water extracts of Senna alexandrina ( S. alexandrina), Peganum harmala ( P. harmala) and Citrullus colocynthis ( C. colocynthis) on Nicotiana benthamiana infected with Cotton Leaf Curl Multan virus were investigated. Results: The results of the antiviral assay revealed that both water and NADES extracts of P. harmala could not exhibit antiviral activity. However, water extracts of S. alexandrina and C. colocynthis successfully inhibited the Cotton Leaf Curl Multan virus , allowing the plant to survive compared to their NADES extracts. High-pressure liquid chromatography (HPLC) and Fourier transform infrared spectroscopy (FTIR) analyses showed higher phenolic and flavonoid content in the water extracts of S. alexandrina, C. colocynthis and P. harmala compared to the NADES extracts. Additionally, both water and NADES extracts of P. harmala showed greater antioxidant and antimicrobial activity against S.aureus in comparison to other extracts, while the NADES extract of C. colocynthis demonstrated higher antimicrobial activity against E.coli strains. Conclusions: Therefore, these results demonstrated that Senna Alexandrina and Citrullus Colocynthis exhibited potent antiviral activity, suggesting that these plants could be therapeutic agent for treating viral infections.
{"title":"Analytical Characterization, Antioxidant, Antiviral and Antimicrobial Potential of Selected Medicinal Plants","authors":"Faiza Nazir, Shah Nawaz-ur-Rehman, Sumayya Khadim, Sania Amber, Fuad Ameen, Naveed Ahmad, Srimathi Priya Lakshminarayanan, Munawar Iqbal","doi":"10.1177/1934578x241274986","DOIUrl":"https://doi.org/10.1177/1934578x241274986","url":null,"abstract":"Objectives/background: The infectious consequences of microorganisms are varied due to resistance to existing antimicrobial drugs or new types of microbes causing lethal consequences for humans. Investigation are being performed to control different viral diseases; therefore; medicinal and herbal plants which have a variety of biological properties, can be evaluated for their anti-viral potential. Methods: The antiviral potential of NADES and water extracts of Senna alexandrina ( S. alexandrina), Peganum harmala ( P. harmala) and Citrullus colocynthis ( C. colocynthis) on Nicotiana benthamiana infected with Cotton Leaf Curl Multan virus were investigated. Results: The results of the antiviral assay revealed that both water and NADES extracts of P. harmala could not exhibit antiviral activity. However, water extracts of S. alexandrina and C. colocynthis successfully inhibited the Cotton Leaf Curl Multan virus , allowing the plant to survive compared to their NADES extracts. High-pressure liquid chromatography (HPLC) and Fourier transform infrared spectroscopy (FTIR) analyses showed higher phenolic and flavonoid content in the water extracts of S. alexandrina, C. colocynthis and P. harmala compared to the NADES extracts. Additionally, both water and NADES extracts of P. harmala showed greater antioxidant and antimicrobial activity against S.aureus in comparison to other extracts, while the NADES extract of C. colocynthis demonstrated higher antimicrobial activity against E.coli strains. Conclusions: Therefore, these results demonstrated that Senna Alexandrina and Citrullus Colocynthis exhibited potent antiviral activity, suggesting that these plants could be therapeutic agent for treating viral infections.","PeriodicalId":19019,"journal":{"name":"Natural Product Communications","volume":"10 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142177270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-07DOI: 10.1177/1934578x241281497
Shewaneh Ayele, Misgana Aragaw, Denberu Kebede, Bayoush Birke, Daniel Bisrat
Objective/Background: Aloe pirottae A. Berger has traditionally been used in Ethiopia to treat various ailments. While previous studies have examined the antimicrobial activity of different parts of A. pirottae against human pathogens, this study aimed to investigate whether its antimicrobial activity extends to plant pathogenic bacteria and fungi. Methods: Compounds were isolated from the leaf latex of A. pirottae using silica gel column chromatography, and their structures were determined using 1H-NMR, 13C-NMR, and ESI-MS spectral data. Both the leaf latex and the isolated compounds were evaluated for their antimicrobial activity against three bacterial ( Pseudomonas syringae pv. gaarcae, Ralstonia solanacearum and Xanthomonas vasicola pv. musacearum) and three fungal ( Fusarium graminarum, Fusaium oxysporum, and Fusarium solani) plant pathogens. Results: A mixture of two diastereoisomeric anthrones was isolated from the leaf latex of A. pirottae and characterized as aloin A/B using 1H-NMR, 13C-NMR, and ESI-MS. Aloin A/B showed the most potent activity against X. vasicola among the bacteria and F. oxysporum among the fungi strains tested, with minimum inhibitory concentrations (MIC) of 3.12 mg/mL and 6.25 mg/mL, respectively. Further exploration uncovered strong binding affinity of aloin A/B towards key proteins in these pathogens, specifically with topoisomerase II (−10.060 kcal/mol) and UDP-N-acetylmuramoyl-L-alanyl-D-glutamate-2, 6-diaminopimelate ligase (murE) (−8.861 kcal/mol) in F. oxysporum and X. vasicola, respectively, through molecular docking studies. Conclusion: The present findings highlight aloin A/B as promising natural antimicrobial agents and lead compounds for new plant pathogen treatments. Further research is recommended to explore their activity against a wider range of plant pathogens.
{"title":"Antimicrobial and Molecular Docking Studies of Anthrones from the Leaf Latex of Aloe pirottae A. Berger against Some Plant Pathogenic Microbes","authors":"Shewaneh Ayele, Misgana Aragaw, Denberu Kebede, Bayoush Birke, Daniel Bisrat","doi":"10.1177/1934578x241281497","DOIUrl":"https://doi.org/10.1177/1934578x241281497","url":null,"abstract":"Objective/Background: Aloe pirottae A. Berger has traditionally been used in Ethiopia to treat various ailments. While previous studies have examined the antimicrobial activity of different parts of A. pirottae against human pathogens, this study aimed to investigate whether its antimicrobial activity extends to plant pathogenic bacteria and fungi. Methods: Compounds were isolated from the leaf latex of A. pirottae using silica gel column chromatography, and their structures were determined using <jats:sup>1</jats:sup>H-NMR, <jats:sup>13</jats:sup>C-NMR, and ESI-MS spectral data. Both the leaf latex and the isolated compounds were evaluated for their antimicrobial activity against three bacterial ( Pseudomonas syringae pv. gaarcae, Ralstonia solanacearum and Xanthomonas vasicola pv. musacearum) and three fungal ( Fusarium graminarum, Fusaium oxysporum, and Fusarium solani) plant pathogens. Results: A mixture of two diastereoisomeric anthrones was isolated from the leaf latex of A. pirottae and characterized as aloin A/B using <jats:sup>1</jats:sup>H-NMR, <jats:sup>13</jats:sup>C-NMR, and ESI-MS. Aloin A/B showed the most potent activity against X. vasicola among the bacteria and F. oxysporum among the fungi strains tested, with minimum inhibitory concentrations (MIC) of 3.12 mg/mL and 6.25 mg/mL, respectively. Further exploration uncovered strong binding affinity of aloin A/B towards key proteins in these pathogens, specifically with topoisomerase II (−10.060 kcal/mol) and UDP-N-acetylmuramoyl-L-alanyl-D-glutamate-2, 6-diaminopimelate ligase (murE) (−8.861 kcal/mol) in F. oxysporum and X. vasicola, respectively, through molecular docking studies. Conclusion: The present findings highlight aloin A/B as promising natural antimicrobial agents and lead compounds for new plant pathogen treatments. Further research is recommended to explore their activity against a wider range of plant pathogens.","PeriodicalId":19019,"journal":{"name":"Natural Product Communications","volume":"88 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142177273","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective: Cydonia oblonga Mill . leaves ethanolic extract (CydOL-EE) has shown different cardioprotective effects. However, no previous studies investigated its direct effect on the vascular smooth muscle tone. Therefore, the study aimed to test the potential vasodilator activity of CydOL-EE in ex-vivo rat thoracic aorta preparations with an additional investigation of its mechanistic effects. Methods: CydOL-EE phytochemical profile was first investigated by HPLC-DAD-ESI-MS/MS and then tested for the vasorelaxation/vasoreactivity effects in rat aortic rings. The NO synthase inhibitor N(ω)-nitro-L-arginine methyl ester (L-NAME) and cyclic guanosine monophosphate inhibitor 1H-[1,2,4]Oxadiazolo[4,3-a]quinoxalin-1-one (ODQ) were used to explore of the involvement of NO-dependent pathways. Results: Chromatographic analysis of CydOL-EE revealed the presence of six flavonols and seven hydroxycinnamic acids. Moreover, CydOL-EE showed a decrease in vasoreactivity caused by dose-dependent phenylephrine (PE) (Control, Emax = 104.29 ± 3.67 vs CydOL-EE, Emax = 70.73 ± 3.67, P < .0001) and a direct relaxing activity to precontraction with PE (Emax = 79.63 ± 3.67%). These responses were abolished during e-NOS inhibition, demonstrating that the mechanism of action was predominately controlled by the participation of an endothelium-dependent system. Conclusion: The results of our study show that CydO-EE demonstrates vasorelaxation and reduction of vasoreactivity through a NO-dependent pathway. These findings provide scientific evidence for further understanding of CydOL-EE use in the treatment of cardiovascular disease.
目的：楙叶乙醇提取物（CydOL-EE）具有不同的心脏保护作用。然而，以前没有研究调查过它对血管平滑肌张力的直接影响。因此，本研究旨在测试 CydOL-EE 在体外大鼠胸主动脉制备中的潜在血管扩张活性，并对其作用机制进行进一步研究。研究方法首先通过 HPLC-DAD-ESI-MS/MS 对 CydOL-EE 植物化学成分进行研究，然后测试其在大鼠主动脉环中的血管舒张/血管活性效应。采用 NO 合酶抑制剂 N（ω）-硝基-L-精氨酸甲酯（L-NAME）和环鸟苷单磷酸抑制剂 1H-[1,2,4]噁二唑并[4,3-a]喹喔啉-1-酮（ODQ）来探讨 NO 依赖性途径的参与。结果CydOL-EE 的色谱分析显示，其中含有六种黄酮醇和七种羟基肉桂酸。此外，CydOL-EE 还能降低剂量依赖性苯肾上腺素（PE）引起的血管收缩活性（对照组，Emax = 104.29 ± 3.67 vs CydOL-EE，Emax = 70.73 ± 3.67，P < .0001），并能直接松弛 PE 的预收缩活性（Emax = 79.63 ± 3.67%）。这些反应在抑制 e-NOS 时消失，表明其作用机制主要受内皮依赖系统的控制。结论我们的研究结果表明，CydO-EE 可通过 NO 依赖性途径起到舒张血管和降低血管活性的作用。这些发现为进一步了解 CydOL-EE 在心血管疾病治疗中的应用提供了科学依据。
{"title":"Vasorelaxant Effects of Ethanolic Extract from Cydonia oblonga Mill. Leaves on Isolated Rat Thoracic Aorta and Potential Mechanism of Action","authors":"Donjeta Krasniqi, Albina Uka, Era Rexhbeqaj, Giangiacomo Beretta, Jasmina Petreska Stanoeva, Bujar Qazimi, Armond Daci","doi":"10.1177/1934578x241282441","DOIUrl":"https://doi.org/10.1177/1934578x241282441","url":null,"abstract":"Objective: Cydonia oblonga Mill . leaves ethanolic extract (CydOL-EE) has shown different cardioprotective effects. However, no previous studies investigated its direct effect on the vascular smooth muscle tone. Therefore, the study aimed to test the potential vasodilator activity of CydOL-EE in ex-vivo rat thoracic aorta preparations with an additional investigation of its mechanistic effects. Methods: CydOL-EE phytochemical profile was first investigated by HPLC-DAD-ESI-MS/MS and then tested for the vasorelaxation/vasoreactivity effects in rat aortic rings. The NO synthase inhibitor N(ω)-nitro-L-arginine methyl ester (L-NAME) and cyclic guanosine monophosphate inhibitor 1H-[1,2,4]Oxadiazolo[4,3-a]quinoxalin-1-one (ODQ) were used to explore of the involvement of NO-dependent pathways. Results: Chromatographic analysis of CydOL-EE revealed the presence of six flavonols and seven hydroxycinnamic acids. Moreover, CydOL-EE showed a decrease in vasoreactivity caused by dose-dependent phenylephrine (PE) (Control, Emax = 104.29 ± 3.67 vs CydOL-EE, Emax = 70.73 ± 3.67, P < .0001) and a direct relaxing activity to precontraction with PE (Emax = 79.63 ± 3.67%). These responses were abolished during e-NOS inhibition, demonstrating that the mechanism of action was predominately controlled by the participation of an endothelium-dependent system. Conclusion: The results of our study show that CydO-EE demonstrates vasorelaxation and reduction of vasoreactivity through a NO-dependent pathway. These findings provide scientific evidence for further understanding of CydOL-EE use in the treatment of cardiovascular disease.","PeriodicalId":19019,"journal":{"name":"Natural Product Communications","volume":"42 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142177291","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-04DOI: 10.1177/1934578x241280831
Mayra Anticona, Daniel Lopez-Malo, Ana Frigola, Jesus Blesa, Maria Jose Esteve
IntroductionCitrus peel, one of the main byproducts of the food industry, is an important source of phenolic compounds with preventive and protective effects. The analysis of these compounds has been widely described, however, information on the purification methods of the extracts is limited.ObjectiveThe objective of the present study is to determine the purification method that favors the obtaining of phenolic compounds from citrus peel extracts.MethodsOranges ( C. sinensis) and mandarins ( C. reticulata) were purchased from a local supermarket (Valencia, Spain). The peels were separated manually and cut into pieces of 25 mm2. An ultrasound-assisted extraction was performed (30 min, 400 W, < 40 °C). Purification by Solid Phase Extraction (SPE) was carried out using cartridges with 200 mg of C18. The QuEChERS procedure was performed using 2 ml DisQUE™ Tubes. The profile of phenolic compounds was analyzed by HPLC-UV.ResultsThe major compounds in the samples were narirutin and hesperidin. Differences were determined according to both purification methods (p < 0.05). Hesperidin was higher in orange peel samples (2229 µg/g FW), while the highest amount of narirutin was obtained in tangerine peel (440 µg/g FW).ConclusionThe sample purification methods are selective. The QuEChERS method showed a greater recovery of phenolic acids and quercetin. The content of phenolic acids was higher in mandarin peel samples.
{"title":"Recovery of Phenolic Compounds from Citrus Peel Through Solid Phase Extraction and QuEChERS as Clean-up Methods","authors":"Mayra Anticona, Daniel Lopez-Malo, Ana Frigola, Jesus Blesa, Maria Jose Esteve","doi":"10.1177/1934578x241280831","DOIUrl":"https://doi.org/10.1177/1934578x241280831","url":null,"abstract":"IntroductionCitrus peel, one of the main byproducts of the food industry, is an important source of phenolic compounds with preventive and protective effects. The analysis of these compounds has been widely described, however, information on the purification methods of the extracts is limited.ObjectiveThe objective of the present study is to determine the purification method that favors the obtaining of phenolic compounds from citrus peel extracts.MethodsOranges ( C. sinensis) and mandarins ( C. reticulata) were purchased from a local supermarket (Valencia, Spain). The peels were separated manually and cut into pieces of 25 mm<jats:sup>2</jats:sup>. An ultrasound-assisted extraction was performed (30 min, 400 W, < 40 °C). Purification by Solid Phase Extraction (SPE) was carried out using cartridges with 200 mg of C<jats:sub>18</jats:sub>. The QuEChERS procedure was performed using 2 ml DisQUE™ Tubes. The profile of phenolic compounds was analyzed by HPLC-UV.ResultsThe major compounds in the samples were narirutin and hesperidin. Differences were determined according to both purification methods (p < 0.05). Hesperidin was higher in orange peel samples (2229 µg/g FW), while the highest amount of narirutin was obtained in tangerine peel (440 µg/g FW).ConclusionThe sample purification methods are selective. The QuEChERS method showed a greater recovery of phenolic acids and quercetin. The content of phenolic acids was higher in mandarin peel samples.","PeriodicalId":19019,"journal":{"name":"Natural Product Communications","volume":"29 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142177274","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-04DOI: 10.1177/1934578x241281568
Aboagye Kwarteng Dofuor
ObjectivePhyto-oxylipins are lipid molecules produced in plants by the oxidative transformation of unsaturated fatty acids via diverse metabolic pathways. Recently, the chemical diversity and functional significance of oxylipins is gaining significant attention. However, the functional significance of these compounds as anti-cancer agents remains largely uncharacterized. The objective of this review is to provide a comprehensive synthesis and analysis of the functional significance of plant oxylipins as anti-cancer agents to facilitate their exploitation in drug discovery and development.MethodsThis review was based on a thorough compilation and analysis of research work carried out on biological significance and implications of plant-derived anti-cancer oxylipins. Curation of data was based on several databases and resources such as Scopus, PubMed, DrugBank and PubChem. Within the context of the scope and subject matter as guided by the objective, no exclusion and inclusion criteria were necessarily employed in the screening of articles.ResultsThe present review explores the origins, anti-cancer properties and functional mechanisms of phyto-oxylipins. The potential functional significance of new and poorly characterized plant oxylipins have also been highlighted. The prospects of plant oxylipins in research, medicine and biotechnology that could optimize their potential are also explored. Insights into the promising avenues that may originate from innovative therapeutic approaches are also discussed.ConclusionDespite the rich source of oxylipins in plants, much of their potential as therapeutic agents for cancer treatment remains to be fully established. Clinical investigations are also needed to determine safe doses and effective delivery methods. Research into phyto-oxylipins require significant attention due to the promise it may hold in addressing key challenges in biotechnology, health, and environmental sustainability.
{"title":"Functional Significance and Implications of Phyto-Oxylipins as Potential Anti-Cancer Agents","authors":"Aboagye Kwarteng Dofuor","doi":"10.1177/1934578x241281568","DOIUrl":"https://doi.org/10.1177/1934578x241281568","url":null,"abstract":"ObjectivePhyto-oxylipins are lipid molecules produced in plants by the oxidative transformation of unsaturated fatty acids via diverse metabolic pathways. Recently, the chemical diversity and functional significance of oxylipins is gaining significant attention. However, the functional significance of these compounds as anti-cancer agents remains largely uncharacterized. The objective of this review is to provide a comprehensive synthesis and analysis of the functional significance of plant oxylipins as anti-cancer agents to facilitate their exploitation in drug discovery and development.MethodsThis review was based on a thorough compilation and analysis of research work carried out on biological significance and implications of plant-derived anti-cancer oxylipins. Curation of data was based on several databases and resources such as Scopus, PubMed, DrugBank and PubChem. Within the context of the scope and subject matter as guided by the objective, no exclusion and inclusion criteria were necessarily employed in the screening of articles.ResultsThe present review explores the origins, anti-cancer properties and functional mechanisms of phyto-oxylipins. The potential functional significance of new and poorly characterized plant oxylipins have also been highlighted. The prospects of plant oxylipins in research, medicine and biotechnology that could optimize their potential are also explored. Insights into the promising avenues that may originate from innovative therapeutic approaches are also discussed.ConclusionDespite the rich source of oxylipins in plants, much of their potential as therapeutic agents for cancer treatment remains to be fully established. Clinical investigations are also needed to determine safe doses and effective delivery methods. Research into phyto-oxylipins require significant attention due to the promise it may hold in addressing key challenges in biotechnology, health, and environmental sustainability.","PeriodicalId":19019,"journal":{"name":"Natural Product Communications","volume":"48 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142177275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective: This study aims to explore the fingerprint and quality control index components of Jiuwei Jiangtang Oral Liquid (JWJT), known for its therapeutic effects on type 2 diabetes mellitus (T2DM). Methods: This study employed network pharmacology and molecular docking to screen the core components, core targets, and pathways of JWJT. Additionally, HPLC fingerprint was established for 12 batches of JWJT. 12 batches of JWJT were evaluated using stoichiometry and common peaks were determined following a similarity assessment. The effective substances were selected to verify the index components of JWJT through assessing their control over glucose consumption in insulin-resistant HepG2 cells and their α-glucosidase inhibitory activity. Results: Through network pharmacology and molecular docking analysis, puerarin, calycosin, ellagic acid, kaempferol-3-O-rutinoside, and kaempferol were identified as core components of JWJT for treating T2DM. Key targets such as AKT1, PIK3R1, INSR, TNF, and EGFR were implicated in regulating pathways including HIF-1, MAPK, and PI3K-Akt in T2DM treatment. The HPLC fingerprints of 12 batches of JWJT samples revealed 10 common peaks, with puerarin, calycosin, ellagic acid, and kaempferol-3-O-rutinoside specifically identified among 4 chromatographic peaks. Using chemical pattern recognition, samples were categorized into two groups, with puerarin, calycosin, and ellagic acid identified as differential markers. Verification through glucose consumption in insulin-resistant HepG2 cells and α-glucosidase inhibitory activity confirmed that puerarin, calycosin, and ellagic acid were active ingredients suitable as quality control indicators for JWJT. Conclusion: The JWJT fingerprint method established in this study is both simple and reproducible. The selected index components will serve as the basis for quality control of JWJT and introduce a novel approach for selecting and verifying quality index components of JWJT in T2DM treatment.
{"title":"Study on Pharmacodynamic Substances and Quality Control of Jiuwei Jiangtang Oral Liquid Based on Network Pharmacology, Fingerprint and In Vitro Experiment","authors":"Qian Li, Jingnan Pei, Li Wang, Jinsha Li, Danjian Qin, Huining Mo, Hongping Zhang","doi":"10.1177/1934578x241280871","DOIUrl":"https://doi.org/10.1177/1934578x241280871","url":null,"abstract":"Objective: This study aims to explore the fingerprint and quality control index components of Jiuwei Jiangtang Oral Liquid (JWJT), known for its therapeutic effects on type 2 diabetes mellitus (T2DM). Methods: This study employed network pharmacology and molecular docking to screen the core components, core targets, and pathways of JWJT. Additionally, HPLC fingerprint was established for 12 batches of JWJT. 12 batches of JWJT were evaluated using stoichiometry and common peaks were determined following a similarity assessment. The effective substances were selected to verify the index components of JWJT through assessing their control over glucose consumption in insulin-resistant HepG2 cells and their α-glucosidase inhibitory activity. Results: Through network pharmacology and molecular docking analysis, puerarin, calycosin, ellagic acid, kaempferol-3-O-rutinoside, and kaempferol were identified as core components of JWJT for treating T2DM. Key targets such as AKT1, PIK3R1, INSR, TNF, and EGFR were implicated in regulating pathways including HIF-1, MAPK, and PI3K-Akt in T2DM treatment. The HPLC fingerprints of 12 batches of JWJT samples revealed 10 common peaks, with puerarin, calycosin, ellagic acid, and kaempferol-3-O-rutinoside specifically identified among 4 chromatographic peaks. Using chemical pattern recognition, samples were categorized into two groups, with puerarin, calycosin, and ellagic acid identified as differential markers. Verification through glucose consumption in insulin-resistant HepG2 cells and α-glucosidase inhibitory activity confirmed that puerarin, calycosin, and ellagic acid were active ingredients suitable as quality control indicators for JWJT. Conclusion: The JWJT fingerprint method established in this study is both simple and reproducible. The selected index components will serve as the basis for quality control of JWJT and introduce a novel approach for selecting and verifying quality index components of JWJT in T2DM treatment.","PeriodicalId":19019,"journal":{"name":"Natural Product Communications","volume":"78 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142177276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：