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Atatürk Baraj Gölü’nde (Türkiye) Yaşayan Carassius gibelio (Bloch, 1782) türünün mtDNA COI ve cyt b Analizi
Pub Date : 2022-09-08 DOI: 10.26650/tjbc.1112782
Arif Parmaksiz, A. Demir
Objective: The aim of this study is to compare the C. gibelio species living in Atatürk Dam Lake with the sequences in the gene bank based on the sequences of the mtDNA COI and Cyt b gene regions and to perform phylogenetic analyzes. Material and Methods: Individuals of the target species living in the Bozova region of Atatürk Dam Lake were randomly selected from fishermen and purchased. Total DNA isolation was performed from muscle tissue using the GeneJET Genomic DNA Purification Kit (Thermo Scientific) according to the protocol instructions. Then, using specific primers of mtDNA COI and Cyt-b regions, the relevant regions were amplified by PCR method. The obtained PCR products were carried out in Agarose gel and the samples forming the product were selected and sent to the commercial firm as service procurement and sequence analysis was performed with the 3500 XL Genetic Analyzer device. Results: Sequences of 620 and 580 bp in length were obtained for the mtDNA COI and cyt b gene regions, respectively. Although the same haplotype was detected for the mtDNA COI region with individuals living in Turkey before, a new haplotype was detected for the mtDNA cyt b gene region that was not previously defined. Conclusion: Sequence analyzes of mtDNA COI and cyt b gene regions of individuals belonging to the C. gibelio species living in Atatürk Dam Lake were performed for the first time in this study and compared with the data in the gene bank.
目的:基于mtDNA COI和Cyt b基因区序列,对atatat rk坝湖生活的C. gibelio物种与基因库中的序列进行比较,并进行系统发育分析。材料和方法:在atatatrk坝湖Bozova地区,从渔民中随机抽取并购买目标物种的个体。根据方案说明,使用GeneJET基因组DNA纯化试剂盒(Thermo Scientific)从肌肉组织中分离总DNA。然后,利用mtDNA COI区和Cyt-b区特异性引物,用PCR方法扩增相关区域。获得的PCR产物在琼脂糖凝胶中进行,选择形成产物的样品作为服务采购送到商业公司,使用3500 XL遗传分析仪设备进行序列分析。结果:mtDNA COI区和cyt b区分别获得了长度为620 bp和580 bp的序列。虽然在之前居住在土耳其的个体中检测到相同的mtDNA COI区域单倍型,但在mtDNA cyt b基因区域检测到一个新的单倍型,这是以前没有定义的。结论:本研究首次对atatatrk坝湖C. gibelio物种个体的mtDNA COI和cyt b基因区进行了序列分析,并与基因库数据进行了比较。
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引用次数: 0
Length-Weight Relationship, Condition Factor, and Gonadosomatic Index of Endemic Alburnus istanbulensis (Battalgil, 1941) in Two Different Habitats: Karamenderes River and Bayramiç Reservoir (Çanakkale, Turkey) Karamenderes河和Bayramiç水库(Çanakkale,土耳其)两种不同生境中伊斯坦堡赤豆(Alburnus istanbulensis,营吉尔,1941)的长权关系、条件因子和性腺指数
Pub Date : 2022-09-08 DOI: 10.26650/tjbc.1161865
N. Partal, Şükran Yalçın Özdilek
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引用次数: 0
New Record of the Pelagic Octopods Argonauta argo (Linnaeus, 1758) off the Shore of the Northern Tip of the Gulf of Aqaba in Jordan 约旦亚喀巴湾北端海岸外的远洋章鱼Argonauta argo (Linnaeus, 1758)的新记录
Pub Date : 2022-08-05 DOI: 10.26650/tjbc.1097163
Ehab Eid, S. Damhoureyeh
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引用次数: 0
A Contribution to the Distribution of Rhynchocalamus melanocephalus (Jan, 1862) in Turkey 对土耳其黑头Rhynchocalamus melanocephalus分布的贡献(1862年1月)
Pub Date : 2022-08-05 DOI: 10.26650/tjbc.1084143
U. Bülbül
{"title":"A Contribution to the Distribution of Rhynchocalamus melanocephalus (Jan, 1862) in Turkey","authors":"U. Bülbül","doi":"10.26650/tjbc.1084143","DOIUrl":"https://doi.org/10.26650/tjbc.1084143","url":null,"abstract":"","PeriodicalId":190602,"journal":{"name":"Turkish Journal of Bioscience and Collections","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126710560","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Length-weight relationships and condition factors of three fish species in the Karamenderes Stream (Çanakkale, Türkiye) Karamenderes河(Çanakkale, t<s:1> rkiye)三种鱼类的长重关系及影响因素
Pub Date : 2022-04-05 DOI: 10.26650/tjbc.20221082587
Şükran Yalçın Özdilek, N. Partal
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引用次数: 2
New record and rare occurrence of European eel (Anguilla anguilla) from freshwater bodies in Karaburun Peninsula (İzmir, Türkiye): Anthropogenic pressures on the fish movements 卡拉布伦半岛淡水水体中欧洲鳗鲡(Anguilla Anguilla)的新记录和罕见现象(İzmir, t<s:1> rkiye):人为对鱼类运动的压力
Pub Date : 2022-04-04 DOI: 10.26650/tjbc.20221084791
S. Ağdamar, Ümit Acar
{"title":"New record and rare occurrence of European eel (Anguilla anguilla) from freshwater bodies in Karaburun Peninsula (İzmir, Türkiye): Anthropogenic pressures on the fish movements","authors":"S. Ağdamar, Ümit Acar","doi":"10.26650/tjbc.20221084791","DOIUrl":"https://doi.org/10.26650/tjbc.20221084791","url":null,"abstract":"","PeriodicalId":190602,"journal":{"name":"Turkish Journal of Bioscience and Collections","volume":"49 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126862202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The Catalog of Aquatic Mollusca (Bivalvia and Gastropoda) in the Limnology Museum of Çanakkale Onsekiz Mart University (COMULM) Çanakkale Onsekiz Mart大学湖沼学博物馆水生软体动物(双壳纲和腹足纲)目录
Pub Date : 2022-03-03 DOI: 10.26650/tjbc.20221017242
Songül Biçer, D. Odabaşı, Buğra Öztürk
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引用次数: 0
Fırat ve Dicle Nehirlerinde Yaşayan Carassius gibelio (Bloch, 1782) Türünün Aminoasit Profilinin Araştırılması
Pub Date : 2022-03-03 DOI: 10.26650/tjbc.20221013455
Arif Parmaksiz, Necati Eneş, Kadir Eği, İsmail Koyuncu
Objective: In this study, data on the amino acid profile of the muscle tissue consumed as food of C. gibelio , an invasive fish living in the Euphrates and Tigris River systems, were obtained. Material and Methods: Muscle tissue samples used for analysis were taken from fish caught and sold with the help of nets by local fishermen. The amino acid profile analysis of the muscle tissue taken as 100 mg/ml from each fish, 29 individuals in total, was made in the LC-MS/MS 8045 device and Jasem LC-MS/MS amino acid analysis kit was used. The results were analyzed and evaluated for the absorbance values obtained from the results of this device. Results: The lowest and highest values and amino acids detected in the fish muscles at a rate of 100 mg/ml of the Euphrates and Tigris groups, respectively; Carnosine with 0.241, Lysine with 668.176 for the Tigris group; For the Euphrates group, it is 3_MHIS with 0.445 and Valin with 9192.287. Conclusion: As a result of this study, an important data set for protein diet was obtained by revealing the amino acid profile of the C. gibelio species living in the Euphrates and Tigris River systems.
目的:本研究对生活在幼发拉底河和底格里斯河水系的入侵鱼类C. gibelio的肌肉组织的氨基酸谱进行了研究。材料和方法:用于分析的肌肉组织样本取自当地渔民用渔网捕获并出售的鱼。每条鱼取肌肉组织100 mg/ml,共29条,在LC-MS/MS 8045装置中进行氨基酸谱分析,使用Jasem LC-MS/MS氨基酸分析试剂盒。对实验结果进行了分析,并对该装置的吸光度值进行了评价。结果:幼发拉底河组和底格里斯河组分别以100 mg/ml的速率在鱼肌肉中检测到最低和最高的氨基酸含量;Tigris组肌肽为0.241,赖氨酸为668.176;幼发拉底河组为3_MHIS 0.445, Valin为9192.287。结论:本研究通过揭示幼发拉底河和底格里斯河水系中C. gibelio物种的氨基酸特征,获得了蛋白质饮食的重要数据集。
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引用次数: 1
The New Maximum Length and Depth of Lagocephalus guentheri Miranda Ribeiro, 1915 in the Mediterranean Sea 1915年地中海Lagocephalus guentheri Miranda Ribeiro发现的最大长度和深度
Pub Date : 2022-03-03 DOI: 10.26650/tjbc.2022987421
Nur Bikem Kesici, C. Dalyan, Onur Gönülal
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引用次数: 0
Invasive freshwater jellyfish has become established in artificial impoundments of Anatolian Peninsula 入侵的淡水水母已经在安纳托利亚半岛的人工蓄水池中建立起来
Pub Date : 2021-09-24 DOI: 10.26650/tjbc.2021984242
A. Tarkan
Objective: A freshwater jellyfish Craspedacusta sowerbii is frequently found in disturbed or artificial bodies of water, e.g. quarry ponds and gravel pits, reservoirs, aquaria and even wastewater treatment facilities, it can also thrive in natural lentic and lotic habitats. In the present study, we present two new records for C. sowerbii which is non-native to Turkey and provide the distribution data of the species in Turkey and Europe. Materials and Methods: The jellyfish samples were obtained from two localities by fishing nets and hand net. Temperature, pH and dissolved oxygen values of the two sampling areas were measured in both of the sampling areas. Results: Non-native freshwater jellyfish Craspedacusta sowerbii is recorded from two different localities namely Akdegirmen Reservoir (Afyon) and Atabey Reservoir (Isparta) in Turkey. Twelve individuals and two individuals of C. sowerbii were sampled in Akdegirmen Reservoir (Afyon) and Atabey Reservoir (Isparta), respectively. Temperature, pH and dissolved oxygen values of the two sampling areas were 18.7oC, 7.9, 6.34 mg/l in Akdegirmen Reservoir, and 10.9oC, 7.94, 9.36 mg/l in Atabey Reservoir. Conclusion: Many studies suggested that it could tolerate a wide range of temperatures from 10°C to 28°C and spread by fish stocking activities as well as by migratory birds in Turkey. It may have been possible for Craspedacusta sowerbii to be introduced to Akdegirmen Reservoir where there are many actively moving cormorants colonies. Also, spread of this species may have been facilitated by fish stocking activities in Turkish inland waters.
目的:淡水水母Craspedacusta sowerbii常出没于受干扰的水体或人工水体中,如采石场、石坑、水库、水族馆甚至污水处理设施等,它也能在自然生境中茁壮成长。本文报道了2个土耳其非原产于的索氏弧菌新记录,并提供了该物种在土耳其和欧洲的分布资料。材料与方法:采用渔网法和手网法在两个地点采集海蜇标本。测量了两个采样区的温度、pH值和溶解氧值。结果:在土耳其Akdegirmen水库(Afyon)和Atabey水库(Isparta)两个不同的地点记录了非本地淡水水母Craspedacusta sowerbii。在Akdegirmen水库(Afyon)和Atabey水库(Isparta)分别采集了12只和2只苏氏库蚊。Akdegirmen水库温度、pH和溶解氧值分别为18.7oC、7.9、6.34 mg/l, Atabey水库温度、pH和溶解氧值分别为10.9oC、7.94、9.36 mg/l。结论:许多研究表明,它可以耐受10°C至28°C的广泛温度,并通过土耳其的鱼类放养活动和候鸟传播。Craspedacusta sowerbii有可能被引入Akdegirmen水库,那里有许多活跃的鸬鹚群落。此外,土耳其内陆水域的鱼类放养活动可能促进了该物种的传播。
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引用次数: 0
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Turkish Journal of Bioscience and Collections
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