Neurogastroenterology and Motility最新文献

Background and aims: Esophagogastric junction (EGJ) integrity influences gastroesophageal reflux disease (GERD) pathophysiology, but its association with positional reflux patterns remains unclear. This study evaluated whether upright and recumbent acid exposure differs based on EGJ status on high-resolution manometry (HRM).

Methods: Retrospective study of patients undergoing HRM and 24-h pH-impedance monitoring off-therapy. EGJ was defined disrupted if any of the following HRM features were present: type II/III morphology, basal pressure < 10.4 mmHg, or EGJ-CI < 9.6 mmHg·cm; intact otherwise. Acid exposure time (AET) and additional reflux metrics were calculated in both positions. Conclusive GERD was defined per Lyon 2.0 (AET > 6%).

Results: Among 283 patients, 102 (36%) had a disrupted EGJ. These patients showed higher total AET (4.7% vs. 1.9% in the intact EGJ, p < 0.001), primarily driven by recumbent exposure (1.9% vs. 0.2%, p < 0.001). In patients with conclusive GERD (n = 91), total and upright AET were similar across EGJ subgroups, while recumbent AET was significantly higher in the disrupted EGJ group (11.5% vs. 3.1%, p < 0.001). Pathologic recumbent AET (> 6%) and severe esophagitis (LA C-D) were observed only in patients with disrupted EGJ (72% vs. 0%, p < 0.001; 14% vs. 0%, p = 0.015, respectively).

Conclusion: EGJ integrity stratifies GERD phenotypes by positional pattern: upright-predominant in patients with intact EGJ and recumbent-predominant in those with disrupted EGJ, the latter also associated with severe esophagitis. This suggests distinct pathophysiologic GERD mechanisms depending on EGJ integrity and may guide personalized management.

Background: Gastric emptying (GE) scintigraphy with 4-h imaging and a radiolabeled chicken egg-based meal (320 kcal, 30% fat) is the standard test for gastroparesis at Mayo Clinic, with an intraindividual coefficient of variation (COV_intra) of 23.8% for GE half emptying time (T_1/2). Alternative meals for patients with dietary restrictions, including egg allergies or vegan diets, are lacking, limiting test accessibility. The study aimed to establish a vegan, lactose-, nut-, and gluten-free meal with similar caloric and fat content to the egg meal and to assess its performance in GE studies.

Methods: In Vitro: ^99mTc binding to egg meal over 4 h was compared to a calorically and nutritionally similar vegan alternative, JUST Egg, using a validated in vitro model simulating gastric conditions of pH, pepsin, and stirring. In Vivo: 10 healthy participants underwent randomized crossover GE scintigraphy with standard and vegan meals, spaced ≥ 3 days apart to measure GE% at 2 h, 4 h, and GE T_1/2. Group comparisons and variance analysis included Rank Sum tests and Bland-Altman plots.

Key results: In Vitro: A strong correlation (R = 0.975, p-value < 0.01) between ^99mTc binding in the egg and vegan egg models was observed. Minimal variance (< 10%) was recorded at all time points. In Vivo: GE at 4 h and GE T_1/2 showed non-statistically significant mean differences, < 2% and < 10 min respectively, between both meals. The intra-individual variability was 18.8%.

Conclusions and inferences: The vegan meal demonstrated in vitro stability, in vivo performance comparable to the egg meal, and a COV_intra of 18.8%, which was lower than the 23.8% reported in repeat egg meal GE studies.

Trial registration: ClinicalTrials.gov number NCT06991036 (https://clinicaltrials.gov/study/NCT06991036?term=NCT06991036&rank=1).

Background: Functional dyspepsia (FD), a disorder of the gut-brain interaction (DGBI), can result in restricted eating habits with associated weight loss. Percutaneous Electrical Nerve Field Stimulation (PENFS), a minimally invasive non-pharmacologic approach, can improve abdominal pain and psychological functioning in children with DGBI. We aimed to analyze changes in anthropometric measures, nutritional intake, and clinical outcomes with PENFS.

Methods: We retrospectively reviewed charts of patients with FD (9-28 years) who restricted oral intake and underwent PENFS. We analyzed validated questionnaires used during treatment. Anthropometric data were also assessed during and after 3 months of treatment. Results were analyzed through linear mixed modeling as least square means.

Key results: Ninety patients reported restricted oral intake, 42% had baseline weight loss and 13% requiring enteral support. Oral intake (p = 0.001), weight (p = 0.02) and BMI (p = 0.002) significantly increased after PENFS. Abdominal pain, nausea, functional disability, depression, and somatization also improved after PENFS. Increased weight Z scores were associated with lower nausea, pain catastrophizing, and depression.

Conclusions and inferences: PENFS improved oral intake, weight, and other anthropometric measures in addition to GI and psychological measures in patients with FD. Changes in anthropometrics were sustained at 3-month follow up. PENFS may be an effective treatment for patients with FD, including children, and restrictive eating to optimize nutritional outcomes. Future studies should include prospective clinical trials and investigate the mechanism of action of PENFS on eating habits.

Background: Recent work suggests an altered duodenal mucosa-associated microbiota (d-MAM) in patients with functional dyspepsia (FD) when compared to controls. This may reflect alterations in host-microbiome homeostasis. Given the specific mucosal immune signatures identified in FD, we hypothesize that these signatures are associated with specific microbial changes. We aim to profile the d-MAM to identify microbes associated with known changes in FD mucosal and peripheral immunity.

Methods: Upper gastrointestinal biopsies were collected from 11 outpatient controls and 17 FD patients. Specific biopsies were collected for 16S rRNA sequencing, histology, and mucosal lamina propria mononuclear cell (LPMC) isolation. Where available, peripheral blood mononuclear cells (PBMC) were isolated. PBMC and LPMC populations were analyzed for T-cell populations by flow cytometry.

Key results: Comparing the histological and immune measures between FD and controls revealed significant differences with decreased villi goblet cells and increased LPMC CD4 Central Memory, LPMC CD8, and PBMC CD4+ Central Memory Th17 in FD patients. Specific microbiome associations found that in controls, villi goblet cells positively correlated with Massilia and negatively with Exiguobacterium. Additionally, controls had a negative correlation between LPMC CD4 Central Memory and Veillonella. Notably, FD patients demonstrated a significant negative correlation between LPMC CD8 and Sulfophobococcus, and a positive correlation between PBMC CD4+ Central Memory Th17 and both Gemella and Fusobacterium.

Conclusions and inferences: Our findings contribute to a growing body of evidence, indicating FD patients exhibit distinct alterations in d-MAM and immune profiles compared to controls. Furthermore, the immune-microbiome associations within control populations were absent in FD patients, suggesting a loss of host-microbiome homeostasis that may contribute to FD pathophysiology.

Background: Autonomic nervous system (ANS) activity is implicated in the pathogenesis of disorders of gut-brain interaction (DGBI). Technological advances enable more accurate investigation of ANS function.

Aim: This study aimed to evaluate the clinical utility of wearable devices in monitoring autonomic and gastrointestinal (GI) function in DGBI.

Methods: A systematic search identified studies in adults with DGBI using wearables to assess heart rate variability (HRV), sleep, skin conductance, or gastric myoelectric activity as clinical readouts for ANS and GI function. The review provides an overview of available devices, while the meta-analysis evaluates consistency in detecting differences between DGBI and healthy controls (HCs). Associations between autonomic function and GI symptom severity were explored. Methodological quality was assessed using the Cochrane risk of bias tool and ROBINS-I. Meta-analyses used random-effects models with standardized mean differences (SMDs).

Results: Thirty-six studies (3 RCTs, 33 observational) involving 3986 DGBI patients were included (HRV: n = 16, sleep: n = 7, gastric myoelectric activity: n = 14, skin conductance: n = 0). Meta-analyses showed lower Root Mean Square of Successive Differences (SMD = -0.503, SE 0.189, 95% CI [-0.873, -0.132]) and percentage of successive RR intervals differing by > 50 ms (SMD = -0.430, SE 0.176, 95% CI [-0.775, -0.085]), reflecting HRV alterations in DGBI versus HCs. No consistent differences were found for other metrics, except normal gastric slow waves (SMD = -0.722, SE 0.216, 95% CI [-1.146, -0.298]). Heterogeneous ANS-symptom associations precluded definitive conclusions.

Conclusions: Wearables show potential for detecting altered ANS and GI function in DGBI, particularly via HRV. Result variability highlights need for further research to confirm accuracy and clinical utility.

Background: Spontaneous neuronal network activity is essential to the functional maturation of central and peripheral circuits, yet whether this is a feature of enteric nervous system development has yet to be established. Although enteric neurons are known exhibit electrophysiological properties early in embryonic development, no connection has been drawn between this neuronal activity and the development of gastrointestinal (GI) motility patterns.

Methods: We use ex vivo GI motility assays with newly developed unbiased computational analyses to identify GI motility patterns across mouse embryonic development.

Key results: We find a previously unknown pattern of neurogenic contractions termed "clustered ripples" that arises spontaneously at embryonic day 16.5, an age earlier than any identified mature GI motility patterns. We further show that these contractions are driven by nicotinic cholinergic signaling.

Conclusions & inferences: Clustered ripples are neurogenic contractile activity that arise from spontaneous ENS activity and precede all known forms of neurogenic GI motility. This earliest motility pattern requires nicotinic cholinergic signaling, which may inform pharmacology for enhancing GI motility in preterm infants.

Cyclic vomiting is a disorder of gut brain interaction (DGBI) emphasizing the need for treatment of both the brain and the gut. Despite clinical success of psychological therapies for CVS, also called brain-gut treatments, an evidence-base is lacking and these treatments are available in few GI practices. This has resulted in an "all guts no brain" approach to CVS. The current paper is a call to action to develop more evidence and use of brain-gut therapies in CVS.

Background and aims: Achalasia classifications, such as the Chicago Classification subtypes based on high-resolution manometry (HRM) and Japanese Esophageal Society (JES), Italian, or Brazilian classifications based on esophagram, have been described. We aimed to compare these schemes for prediction of achalasia treatment outcomes.

Methods: 222 adult patients with achalasia that completed pretreatment HRM and esophagram before and after treatment were included. Pretreatment HRM achalasia subtypes were determined by the Chicago Classification and JES; Italian and Brazilian classifications were defined by pretreatment esophagram. Post-treatment outcomes were defined using the Eckardt symptom score (good outcome < 4) or timed barium esophagram (TBE; good outcome 5-min column height < 5 cm).

Results: The Chicago Classification was a significant predictor of symptomatic outcome (p = 0.003-0.007), whereas JES, Italian, and Brazilian schemes were not. All four classifications were significant predictors of radiographic outcome, with JES demonstrating the best model fit as identified by lowest Akaike information criteria (AIC). Type III achalasia (Chicago Classification) patients had the lowest rates of good symptomatic outcomes despite treatment primarily with POEM, whereas advanced esophagram stages (JES-C, Italian IV, Brazilian 2-3) were associated with poorer radiographic outcomes.

Conclusions: Both HRM- and esophagram-based classification schemes predict achalasia treatment outcomes, though with different strengths. While treatment choice may impact outcomes, HRM best predicted symptomatic outcomes, while esophagram classifications better predicted objective radiographic outcomes. Utilizing both modalities may enhance prognostication of outcomes in achalasia.