Aim. To develop the composition and laboratory technology of a therapeutic and preventive mouthwash based on a plant active ingredient and study a number of its quality indicators. Materials and methods. When developing the composition and technology of the drug, active pharmaceutical ingredients (APIs) and excipients approved for medical use and widely used in pharmaceutical practice were selected. The information-search, information-analytical, organoleptic, physicochemical, pharmacotechnological and microbiological research methods were used. Statistical processing of the data obtained was performed using Microsoft Excel 2007 spreadsheets. Results and discussion. The composition and number of components of the therapeutic and preventive mouthwash have been theoretically substantiated. The theoretical and experimental substantiation of the composition of the original mouthwash of the multifaceted action with a deodorizing effect for use in halitosis (the liquid extract of burdock, pot marigold, fleaworts, horsetail, sage, inula, chicory (45 : 5 : 15 : 5 : 10 : 15 : 5, respectively) (1 : 4); thyme and lemon essential oil, xylitol, polysorbate-20, glycerin, PEG-40 hydrogenated castor oil, purified water) is given. The tests showed the need to add antimicrobial preservatives to the mouthwash composition. Taking into account the requirements of safety and cost-effectiveness, as well as slightly higher antimicrobial activity, potassium sorbate in the minimum effective concentration of 0.1 % was chosen as a promising preservative. Salivary diagnostics revealed a general tendency to improve the physicochemical properties of saliva by a number of parameters after applying an experimental sample of the mouthwash developed. There is a general tendency to improve the physicochemical properties of saliva by a number of parameters after using an experimental sample of the mouthwash developed. Conclusions. The composition of the therapeutic and preventive original drug – a mouthwash of the multifaceted action with the antimicrobial and deodorizing effects for use in halitosis has been developed.
{"title":"The theoretical and experimental substantiation of the composition of a mouthwash","authors":"O. Matsiuk, O. Kaliuzhnaia, L. Vyshnevska","doi":"10.24959/nphj.23.109","DOIUrl":"https://doi.org/10.24959/nphj.23.109","url":null,"abstract":"Aim. To develop the composition and laboratory technology of a therapeutic and preventive mouthwash based on a plant active ingredient and study a number of its quality indicators.\u0000Materials and methods. When developing the composition and technology of the drug, active pharmaceutical ingredients (APIs) and excipients approved for medical use and widely used in pharmaceutical practice were selected. The information-search, information-analytical, organoleptic, physicochemical, pharmacotechnological and microbiological research methods were used. Statistical processing of the data obtained was performed using Microsoft Excel 2007 spreadsheets.\u0000Results and discussion. The composition and number of components of the therapeutic and preventive mouthwash have been theoretically substantiated. The theoretical and experimental substantiation of the composition of the original mouthwash of the multifaceted action with a deodorizing effect for use in halitosis (the liquid extract of burdock, pot marigold, fleaworts, horsetail, sage, inula, chicory (45 : 5 : 15 : 5 : 10 : 15 : 5, respectively) (1 : 4); thyme and lemon essential oil, xylitol, polysorbate-20, glycerin, PEG-40 hydrogenated castor oil, purified water) is given. The tests showed the need to add antimicrobial preservatives to the mouthwash composition. Taking into account the requirements of safety and cost-effectiveness, as well as slightly higher antimicrobial activity, potassium sorbate in the minimum effective concentration of 0.1 % was chosen as a promising preservative. Salivary diagnostics revealed a general tendency to improve the physicochemical properties of saliva by a number of parameters after applying an experimental sample of the mouthwash developed. There is a general tendency to improve the physicochemical properties of saliva by a number of parameters after using an experimental sample of the mouthwash developed.\u0000Conclusions. The composition of the therapeutic and preventive original drug – a mouthwash of the multifaceted action with the antimicrobial and deodorizing effects for use in halitosis has been developed.","PeriodicalId":19221,"journal":{"name":"News of Pharmacy","volume":"4 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87630153","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Zhadko, I. Pestun, Z. M. Mnushko, G. S. Babicheva, O. Y. Rohulia
Aim. To study the awareness of graduate pharmacy students with the concept of the “rational use of medicines” and related issues. Materials and methods. A questionnaire survey of 172 graduate students was conducted at the National University of Pharmacy. Results and discussion. Most students believe that they are clearly familiar with the concept of the “rational use of medicines”; 97.1 % of them know it from the disciplines they studied at the University. It has been found that students have both correct and erroneous ideas about the mandatory principles of the rational use of medicines; they are most aware of its clinical aspects. Not all signs of the irrational use of medicines are obvious for future pharmacists. Students possess certain skills that can be useful in promoting the rational use of medicines. However, not all acquired skills are in demand in the professional activities of pharmacists in Ukraine. 75.6% of respondents think their knowledge gained at the University is sufficient to promote the rational use of medicines. Only 48.3 % of respondents believe that Ukrainian pharmacists are sufficiently involved in promoting the rational use of medicines. Conclusions. The study has revealed the lack of awareness of some pharmaceutical students about the concept and problem of the rational use of medicines, as well as the gap between the acquired skills and their demand in professional activities. Less than half of students rate the role of pharmacists in promoting the rational use of medicines at a sufficient level. Further research will be aimed at developing measures to improve the awareness of pharmacy students with the basic concepts of the rational use of medicines, as well as expanding the participation of Ukrainian pharmacists in promoting the rational use of medicines.
{"title":"The assessment of awareness about the rational use of medicines among ukrainian pharmacy students","authors":"S. Zhadko, I. Pestun, Z. M. Mnushko, G. S. Babicheva, O. Y. Rohulia","doi":"10.24959/nphj.23.100","DOIUrl":"https://doi.org/10.24959/nphj.23.100","url":null,"abstract":"Aim. To study the awareness of graduate pharmacy students with the concept of the “rational use of medicines” and related issues.\u0000Materials and methods. A questionnaire survey of 172 graduate students was conducted at the National University of Pharmacy.\u0000Results and discussion. Most students believe that they are clearly familiar with the concept of the “rational use of medicines”; 97.1 % of them know it from the disciplines they studied at the University. It has been found that students have both correct and erroneous ideas about the mandatory principles of the rational use of medicines; they are most aware of its clinical aspects. Not all signs of the irrational use of medicines are obvious for future pharmacists. Students possess certain skills that can be useful in promoting the rational use of medicines. However, not all acquired skills are in demand in the professional activities of pharmacists in Ukraine. 75.6% of respondents think their knowledge gained at the University is sufficient to promote the rational use of medicines. Only 48.3 % of respondents believe that Ukrainian pharmacists are sufficiently involved in promoting the rational use of medicines.\u0000Conclusions. The study has revealed the lack of awareness of some pharmaceutical students about the concept and problem of the rational use of medicines, as well as the gap between the acquired skills and their demand in professional activities. Less than half of students rate the role of pharmacists in promoting the rational use of medicines at a sufficient level. Further research will be aimed at developing measures to improve the awareness of pharmacy students with the basic concepts of the rational use of medicines, as well as expanding the participation of Ukrainian pharmacists in promoting the rational use of medicines.","PeriodicalId":19221,"journal":{"name":"News of Pharmacy","volume":"14 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84872228","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Aim. To select the concentration of fennel essential oil as an antimicrobial component and amaranth oil as an antioxidant component in the composition of a wound healing ointment for the wound therapy in the second and third phases of the wound process. Materials and methods. The study objects were model samples of the ointment with the lipophilic extract of marigold, amaranth oil and fennel essential oil. The antibacterial and antifungal activity of the model samples of the ointment was determined in experiments in vitro by the agar diffusion method. The study of antioxidant properties was performed by biotesting on the biological model of Paramecium caudatum infusoria grown in the Lozin-Lozinsky nutrient medium at a temperature of 20-26 °С. The results were statistically processed using the Excel for Windows software. Results and discussion. The results of the studies conducted showed the presence of the antimicrobial action of varying degrees for all ointment samples with different concentrations of fennel essential oil. There was a slow increase in the antimicrobial activity of ointments with an increase in the concentration of fennel essential oil. The promising concentrations of fennel essential oil providing the maximum antimicrobial effect in the composition of the wound healing ointment were 1.5 % and 2.0 %. Taking into account a slight difference in the growth retardation zones and the requirements of safety and cost-effectiveness 1.5 % concentration of fennel essential oil was chosen for further research. Exposure of the experimental samples with different concentrations of amaranth oil led to a prolongation of the period of activity of infusoria under the influence of poisons compared to the control, indicating the presence of a high level of antioxidant activity. Conclusions. Based on the microbiological studies and biotesting, the concentration of antimicrobial and antioxidant components (fennel essential oil – 1.5 %, amaranth oil – 8 %) has been substantiated when developing a wound healing ointment for the therapy in the second and third phases of the wound process.
的目标。在伤口愈合软膏的组合物中选择茴香精油作为抗菌成分和苋菜油作为抗氧化成分的浓度，用于伤口处理的第二和第三阶段的伤口治疗。材料和方法。以万寿菊、苋菜油和茴香精油的亲脂提取物为模型样品进行研究。体外实验采用琼脂扩散法测定软膏模型样品的抗菌和抗真菌活性。对在Lozin-Lozinsky营养培养基中培养的尾状草履虫(parecium caudatum inusoria)生物模型进行生物试验，实验温度为20-26°С。使用Excel for Windows软件对结果进行统计处理。结果和讨论。研究结果表明，不同浓度茴香精油对所有药膏样品均有不同程度的抗菌作用。随着茴香精油浓度的增加，药膏的抗菌活性缓慢增加。在伤口愈合软膏组合物中，茴香精油的浓度为1.5%和2.0%，具有最大的抗菌效果。考虑到生长阻滞区的细微差异以及安全性和成本效益的要求，选择浓度为1.5%的茴香精油进行进一步研究。实验样品暴露于不同浓度的苋菜油中，与对照相比，毒素影响下的虫体活性期延长，表明存在高水平的抗氧化活性。基于微生物学研究和生物测试，抗菌和抗氧化成分(茴香精油- 1.5%，苋菜油- 8%)的浓度在开发伤口愈合软膏用于伤口过程的第二和第三阶段的治疗时得到了证实。
{"title":"Microbiological studies in the development of wound healing ointment","authors":"O. I. Trembach, N. Khokhlenkova","doi":"10.24959/nphj.23.108","DOIUrl":"https://doi.org/10.24959/nphj.23.108","url":null,"abstract":"Aim. To select the concentration of fennel essential oil as an antimicrobial component and amaranth oil as an antioxidant component in the composition of a wound healing ointment for the wound therapy in the second and third phases of the wound process.\u0000Materials and methods. The study objects were model samples of the ointment with the lipophilic extract of marigold, amaranth oil and fennel essential oil. The antibacterial and antifungal activity of the model samples of the ointment was determined in experiments in vitro by the agar diffusion method. The study of antioxidant properties was performed by biotesting on the biological model of Paramecium caudatum infusoria grown in the Lozin-Lozinsky nutrient medium at a temperature of 20-26 °С. The results were statistically processed using the Excel for Windows software.\u0000Results and discussion. The results of the studies conducted showed the presence of the antimicrobial action of varying degrees for all ointment samples with different concentrations of fennel essential oil. There was a slow increase in the antimicrobial activity of ointments with an increase in the concentration of fennel essential oil. The promising concentrations of fennel essential oil providing the maximum antimicrobial effect in the composition of the wound healing ointment were 1.5 % and 2.0 %. Taking into account a slight difference in the growth retardation zones and the requirements of safety and cost-effectiveness 1.5 % concentration of fennel essential oil was chosen for further research. Exposure of the experimental samples with different concentrations of amaranth oil led to a prolongation of the period of activity of infusoria under the influence of poisons compared to the control, indicating the presence of a high level of antioxidant activity.\u0000Conclusions. Based on the microbiological studies and biotesting, the concentration of antimicrobial and antioxidant components (fennel essential oil – 1.5 %, amaranth oil – 8 %) has been substantiated when developing a wound healing ointment for the therapy in the second and third phases of the wound process.","PeriodicalId":19221,"journal":{"name":"News of Pharmacy","volume":"27 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81955801","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
O. Ruban, D. S. Puliaiev, N. Khokhlenkova, O. Ivanyuk
Aim. To analyze the scientific literature data on the use of three-dimensional technologies in pharmacy, in particular in the technology of oral solid dosage forms, the advantages and disadvantages of 3D printing, and current research on the development of drugs using 3D printing. Materials and methods. To achieve this goal, bibliosemantic and analytical methods were used. Results and discussion. Personalized medicine is currently a major area of development in the healthcare sector. Its goal is to adapt treatment to a specific person, taking into account the peculiarities of the physiology, drug reactions, and the genetic profile. There are technologies that contribute to the paradigm shift from the traditional “one-size-fits-all”approach to personalized medicine, the main one being three-dimensional (3D) printing. 3D printing involves the creation of a three-dimensional object using various computer programs. 3D printing can be used to create a wide range of dosage forms that differ in shape, release profile, and drug combination. The main 3D printing technology platforms under study in the pharmaceutical sector include inkjet printing, binder inkjet printing, fused filament manufacturing, selective laser sintering, stereolithography, and injection microsyringing. Conclusions. The article discusses various 3D printing technologies and the prospects for their application in research on the development of new medicines, in particular oral solid dosage forms, and highlights their advantages and disadvantages.
{"title":"The use of three-dimensional technologies in the development of oral solid dosage forms","authors":"O. Ruban, D. S. Puliaiev, N. Khokhlenkova, O. Ivanyuk","doi":"10.24959/nphj.23.112","DOIUrl":"https://doi.org/10.24959/nphj.23.112","url":null,"abstract":"Aim. To analyze the scientific literature data on the use of three-dimensional technologies in pharmacy, in particular in the technology of oral solid dosage forms, the advantages and disadvantages of 3D printing, and current research on the development of drugs using 3D printing.\u0000Materials and methods. To achieve this goal, bibliosemantic and analytical methods were used.\u0000Results and discussion. Personalized medicine is currently a major area of development in the healthcare sector. Its goal is to adapt treatment to a specific person, taking into account the peculiarities of the physiology, drug reactions, and the genetic profile. There are technologies that contribute to the paradigm shift from the traditional “one-size-fits-all”approach to personalized medicine, the main one being three-dimensional (3D) printing. 3D printing involves the creation of a three-dimensional object using various computer programs. 3D printing can be used to create a wide range of dosage forms that differ in shape, release profile, and drug combination. The main 3D printing technology platforms under study in the pharmaceutical sector include inkjet printing, binder inkjet printing, fused filament manufacturing, selective laser sintering, stereolithography, and injection microsyringing.\u0000Conclusions. The article discusses various 3D printing technologies and the prospects for their application in research on the development of new medicines, in particular oral solid dosage forms, and highlights their advantages and disadvantages.","PeriodicalId":19221,"journal":{"name":"News of Pharmacy","volume":"33 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81035184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Self-emulsifying compositions are the basis of oral drug delivery systems used to improve the solubility and increase the bioavailability of active pharmaceutical ingredients that are poorly soluble in the aqueous medium of gastric juice. Aim. To develop self-emulsifying compositions using simvastatin as an active pharmaceutical ingredient. Materials and methods. During the development of the composition of the self-emulsifying mixture, excipients, such as solvents, co-solvents, surfactants and co-surfactants, allowed in pharmaceutical production were used. The studies of solubility, the formation rate of emulsions of the self-emulsifying composition and stability were carried out by generally accepted methods according to the methods of the SPhU and other valid normative documents. Results and discussion. The studies of the solubility of simvastatin allowed choosing a mixture of castor oil and PEG-40 GRO as a solvent, which improved the solubility of the substance in oil. It was determined that it was efficient to use Tween-80 as the main surfactant. Glycerol monostearate, distilled monoglycerides, polyethylene oxide-400, polyethylene oxide-1500 and polyethylene glycol-100 stearate were introduced into the composition as co-surfactants. It was found that the compositions, which included polyethylene oxide-400 and polyethylene oxide-1500, had significantly worse indicators of the formation rate of emulsions than the rest of the samples. The sample containing distilled monoglycerides did not withstand a decrease in the pH value of the medium. Conclusions. According to the results obtained, samples containing castor oil, PEG-40 hydrogenated castor oil, Tween-80, glycerol monostearate or PEG-100 stearate were selected for further research.
{"title":"The study on the development of self-emulsifying compositions with simvastatin","authors":"L. Bodnar, N. Polovko","doi":"10.24959/nphj.23.104","DOIUrl":"https://doi.org/10.24959/nphj.23.104","url":null,"abstract":"Self-emulsifying compositions are the basis of oral drug delivery systems used to improve the solubility and increase the bioavailability of active pharmaceutical ingredients that are poorly soluble in the aqueous medium of gastric juice. Aim. To develop self-emulsifying compositions using simvastatin as an active pharmaceutical ingredient. Materials and methods. During the development of the composition of the self-emulsifying mixture, excipients, such as solvents, co-solvents, surfactants and co-surfactants, allowed in pharmaceutical production were used. The studies of solubility, the formation rate of emulsions of the self-emulsifying composition and stability were carried out by generally accepted methods according to the methods of the SPhU and other valid normative documents. Results and discussion. The studies of the solubility of simvastatin allowed choosing a mixture of castor oil and PEG-40 GRO as a solvent, which improved the solubility of the substance in oil. It was determined that it was efficient to use Tween-80 as the main surfactant. Glycerol monostearate, distilled monoglycerides, polyethylene oxide-400, polyethylene oxide-1500 and polyethylene glycol-100 stearate were introduced into the composition as co-surfactants. It was found that the compositions, which included polyethylene oxide-400 and polyethylene oxide-1500, had significantly worse indicators of the formation rate of emulsions than the rest of the samples. The sample containing distilled monoglycerides did not withstand a decrease in the pH value of the medium. Conclusions. According to the results obtained, samples containing castor oil, PEG-40 hydrogenated castor oil, Tween-80, glycerol monostearate or PEG-100 stearate were selected for further research.","PeriodicalId":19221,"journal":{"name":"News of Pharmacy","volume":"43 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87869069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Aim. To study the historical aspects of soap making, technological stages and methods, the main chemical processes accompanying the soap formation, and the peculiarities of choosing components for its manufacture. Materials and methods. Methods of information search, comparison and system analysis, and data generalization were used for research. Materials of scientific databases (Scopus, PubMed, Web of Science, and Google Scholar) were used. Results and discussion. The use of soap as a hygiene product began long before our era and was successfully integrated into our daily life in accordance with the modern needs of humankind. Industrial production of liquid, solid and dry soap is currently established and widely scaled. However, the content of synthetic components makes it impossible to use such soap for therapeutic and preventive purposes. Small “home” soap factories use many natural components and even refuse to add preservatives, synthetic dyes and fragrances, but these are soaps for daily hygiene. Conclusions. Therefore, the development of the composition and technology of soap for the complex treatment or prevention of dermatological diseases based on natural components is relevant.
的目标。研究肥皂制造的历史、工艺阶段和方法、肥皂形成的主要化学过程以及制造肥皂的原料选择的特点。材料和方法。采用资料检索、比较与系统分析、数据归纳等方法进行研究。使用科学数据库(Scopus, PubMed, Web of Science，谷歌Scholar)的资料。结果和讨论。肥皂作为卫生用品的使用早在我们这个时代之前就开始了，并根据人类的现代需求成功地融入了我们的日常生活。工业生产的液体，固体和干肥皂目前已建立和广泛的规模。然而，合成成分的含量使得这种肥皂不可能用于治疗和预防目的。小型“家用”肥皂厂使用许多天然成分，甚至拒绝添加防腐剂、合成染料和香料，但这些肥皂是为了日常卫生。因此，开发以天然成分为基础的复合治疗或预防皮肤病的肥皂成分和技术具有重要意义。
{"title":"The bibliosemantic analysis of the soap application in pharmacy and current trends in its composition and manufacturing technology","authors":"I. V. Honcharov, L. Vyshnevska","doi":"10.24959/nphj.23.103","DOIUrl":"https://doi.org/10.24959/nphj.23.103","url":null,"abstract":"Aim. To study the historical aspects of soap making, technological stages and methods, the main chemical processes accompanying the soap formation, and the peculiarities of choosing components for its manufacture.\u0000Materials and methods. Methods of information search, comparison and system analysis, and data generalization were used for research. Materials of scientific databases (Scopus, PubMed, Web of Science, and Google Scholar) were used.\u0000Results and discussion. The use of soap as a hygiene product began long before our era and was successfully integrated into our daily life in accordance with the modern needs of humankind. Industrial production of liquid, solid and dry soap is currently established and widely scaled. However, the content of synthetic components makes it impossible to use such soap for therapeutic and preventive purposes. Small “home” soap factories use many natural components and even refuse to add preservatives, synthetic dyes and fragrances, but these are soaps for daily hygiene.\u0000Conclusions. Therefore, the development of the composition and technology of soap for the complex treatment or prevention of dermatological diseases based on natural components is relevant.","PeriodicalId":19221,"journal":{"name":"News of Pharmacy","volume":"5 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86364222","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The toxicological significance of antidepressant drugs is steadily increasing. Development of the effective methods of the sample preparation is a topical issue of the chemical-toxicological analysis of antidepressants. Aim. To develop conditions for isolating the antidepressant sertraline from the blood and urine by the liquid-liquid extraction. Materials and methods. The studies were performed with model blood and urine samples spiked with sertraline. In the study of the blood, the formed blood elements were pre-precipitated by adding a 10 % solution of trichloroacetic acid. Extraction purification was performed with hexane at pH 1, and the drug was extracted from biological fluids with chloroform at pH 8. The extracts obtained were further subjected to the TLC purification. Determination of sertraline in eluates from chromatograms was performed by the UV spectrophotometric method. Results and discussion. The Rf value of sertraline in the mobile phase of methanol – 25 % ammonium hydroxide solution (100:10.5) was 0.42 ± 0.02. The UV spectra of the eluates from the chromatograms had absorption maxima at wavelengths of 268 ± 2, 275 ± 2 and 283 ± 2 nm and matched with the UV spectrum of a standard solution of sertraline in 0.1 mole/L ethanol solution of hydrochloric acid. The quantitative determination was performed at λmax 275 nm according to the equation of the calibration curve y = 0,00216x–0,03. The methods developed allowed to isolate 80.0 ± 4.0 % of sertraline from the urine, 39.0 ± 2.0 % from the blood serum, and additionally 8.0 ± 0.7 % of the antidepressant studiedfrom the blood sediment after its separation from the blood serum. Conclusions. The effective conditions for the sample preparation of biological fluids using the liquid-liquid extraction method for the antidepressant sertraline have been determined. The results obtained are of practical importance for creating an algorithm for the toxicological study of biological samples for the presence of this antidepressant in case of fatal drug intoxications.
{"title":"Development of conditions for sertraline isolation from biological fluids","authors":"S. V. Baiurka, S. A. Karpushyna","doi":"10.24959/nphj.23.107","DOIUrl":"https://doi.org/10.24959/nphj.23.107","url":null,"abstract":"The toxicological significance of antidepressant drugs is steadily increasing. Development of the effective methods of the sample preparation is a topical issue of the chemical-toxicological analysis of antidepressants.\u0000Aim. To develop conditions for isolating the antidepressant sertraline from the blood and urine by the liquid-liquid extraction.\u0000Materials and methods. The studies were performed with model blood and urine samples spiked with sertraline. In the study of the blood, the formed blood elements were pre-precipitated by adding a 10 % solution of trichloroacetic acid. Extraction purification was performed with hexane at pH 1, and the drug was extracted from biological fluids with chloroform at pH 8. The extracts obtained were further subjected to the TLC purification. Determination of sertraline in eluates from chromatograms was performed by the UV spectrophotometric method.\u0000Results and discussion. The Rf value of sertraline in the mobile phase of methanol – 25 % ammonium hydroxide solution (100:10.5) was 0.42 ± 0.02. The UV spectra of the eluates from the chromatograms had absorption maxima at wavelengths of 268 ± 2, 275 ± 2 and 283 ± 2 nm and matched with the UV spectrum of a standard solution of sertraline in 0.1 mole/L ethanol solution of hydrochloric acid. The quantitative determination was performed at λmax 275 nm according to the equation of the calibration curve y = 0,00216x–0,03. The methods developed allowed to isolate 80.0 ± 4.0 % of sertraline from the urine, 39.0 ± 2.0 % from the blood serum, and additionally 8.0 ± 0.7 % of the antidepressant studiedfrom the blood sediment after its separation from the blood serum.\u0000Conclusions. The effective conditions for the sample preparation of biological fluids using the liquid-liquid extraction method for the antidepressant sertraline have been determined. The results obtained are of practical importance for creating an algorithm for the toxicological study of biological samples for the presence of this antidepressant in case of fatal drug intoxications.","PeriodicalId":19221,"journal":{"name":"News of Pharmacy","volume":"31 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75278569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
I. Vlasova, I. V. Botsula, I. Kireyev, O. Koshovyi
Inflammation accompanies huge numbers of diseases. In case of inflammation, non-steroidal anti-inflammatory drugs are used, but they have a number of side effects and disadvantages. In turn, medicines based on the medicinal plant raw material that exhibit the anti-inflammatory activity, on the contrary, are underestimated and insufficientlystudied. An interesting object in this direction is large cranberry leaves of Ericaceae family. Aim. To study the chemical composition and the anti-inflammatory activity of extracts from large cranberry leaves to establish the prospect of creating new drugs with the anti-inflammatory activity. Materials and methods. The study objects were dry extracts from large cranberry (Oxycoccus macrocarpus (Ait.)) leaves. The chemical analysis of the extracts was studied by TLC and spectrophotometry methods. The anti-inflammatory activity of the extracts was studied on the model of carrageenan edema. Results and discussion. Hyperoside and chlorogenic acid were identified in the extracts. In the dry extract from large cranberry leaves, the content of the total amount of derivatives of hydroxycinnamic compounds was 11.60 ± 0.05 %, and in the modified extract – 3.09 ± 0.05 %. The anti-exudative activity was more pronounced in the group of animals that received the cranberry extract with arginine in the dose of 100 mg/kg. Conclusions. Identification and the quantification of biologically active substances in large cranberry leaves extracts have been carried out; their anti-inflammatory activity has been determined.
{"title":"The study of the chemical composition and the anti-inflammatory activity of dry extracts from large cranberry leaves","authors":"I. Vlasova, I. V. Botsula, I. Kireyev, O. Koshovyi","doi":"10.24959/nphj.23.106","DOIUrl":"https://doi.org/10.24959/nphj.23.106","url":null,"abstract":"Inflammation accompanies huge numbers of diseases. In case of inflammation, non-steroidal anti-inflammatory drugs are used, but they have a number of side effects and disadvantages. In turn, medicines based on the medicinal plant raw material that exhibit the anti-inflammatory activity, on the contrary, are underestimated and insufficientlystudied. An interesting object in this direction is large cranberry leaves of Ericaceae family.\u0000Aim. To study the chemical composition and the anti-inflammatory activity of extracts from large cranberry leaves to establish the prospect of creating new drugs with the anti-inflammatory activity.\u0000Materials and methods. The study objects were dry extracts from large cranberry (Oxycoccus macrocarpus (Ait.)) leaves. The chemical analysis of the extracts was studied by TLC and spectrophotometry methods. The anti-inflammatory activity of the extracts was studied on the model of carrageenan edema.\u0000Results and discussion. Hyperoside and chlorogenic acid were identified in the extracts. In the dry extract from large cranberry leaves, the content of the total amount of derivatives of hydroxycinnamic compounds was 11.60 ± 0.05 %, and in the modified extract – 3.09 ± 0.05 %. The anti-exudative activity was more pronounced in the group of animals that received the cranberry extract with arginine in the dose of 100 mg/kg.\u0000Conclusions. Identification and the quantification of biologically active substances in large cranberry leaves extracts have been carried out; their anti-inflammatory activity has been determined.","PeriodicalId":19221,"journal":{"name":"News of Pharmacy","volume":"250 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80713810","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Aim. To analyze the existing methods for solving multi-criteria optimization problems with quantitative factors and consider the expediency of their application in the pharmaceutical technological research. Materials and methods. The theoretical and empirical methods, as well as scientific data on multi-criteria optimization methods were used in the research. Results and discussion. It has been found that when conducting the research with quantitative factors the statistical methods for processing the results of experiments are used. It has been determined that the regression analysis allows us to present the dynamics of changes in the pharmacopoeial characteristics studied from variable quantitative factors in a compact form. It has been found that regression equations enable the researcher to effectively search for optimal conditions for conducting technological operations and are local criteria for finding the optimal solution. It has been proven that the need to optimize several criteria simultaneously when developing the technology of a medicinal product allows considering the tasks of multi-criteria selection in the pharmaceutical research as a special class of decision-making problems that are on the verge between the research of operations for well-structured quantitative situations and decision-making tasks, which methods of solving differ. Conclusions. To determine the optimal solution in the course of the pharmaco-technological development, a method for finding an ideal point has been proposed as a synthesis of mathematical calculations and decision-making procedures by a researcher. The search is conducted in the range of acceptable values of variables and determines their totality, which is able to provide a set of values of pharmaco-technological criteria that is closest to the optimal option determined by the researcher. This approach involves preliminary solution of single-criterion optimization problems for each individual criterion and reduction of all to a mathematical form that determines the minimum deviation of the objective functions obtained from the optimal values.
{"title":"A methodical approach to the multi-criteria selection in the pharmaceutical technological research with quantitative factors","authors":"O. Kutova, R. Sahaidak-Nikitiuk","doi":"10.24959/nphj.23.105","DOIUrl":"https://doi.org/10.24959/nphj.23.105","url":null,"abstract":"Aim. To analyze the existing methods for solving multi-criteria optimization problems with quantitative factors and consider the expediency of their application in the pharmaceutical technological research.\u0000Materials and methods. The theoretical and empirical methods, as well as scientific data on multi-criteria optimization methods were used in the research.\u0000Results and discussion. It has been found that when conducting the research with quantitative factors the statistical methods for processing the results of experiments are used. It has been determined that the regression analysis allows us to present the dynamics of changes in the pharmacopoeial characteristics studied from variable quantitative factors in a compact form. It has been found that regression equations enable the researcher to effectively search for optimal conditions for conducting technological operations and are local criteria for finding the optimal solution. It has been proven that the need to optimize several criteria simultaneously when developing the technology of a medicinal product allows considering the tasks of multi-criteria selection in the pharmaceutical research as a special class of decision-making problems that are on the verge between the research of operations for well-structured quantitative situations and decision-making tasks, which methods of solving differ.\u0000Conclusions. To determine the optimal solution in the course of the pharmaco-technological development, a method for finding an ideal point has been proposed as a synthesis of mathematical calculations and decision-making procedures by a researcher. The search is conducted in the range of acceptable values of variables and determines their totality, which is able to provide a set of values of pharmaco-technological criteria that is closest to the optimal option determined by the researcher. This approach involves preliminary solution of single-criterion optimization problems for each individual criterion and reduction of all to a mathematical form that determines the minimum deviation of the objective functions obtained from the optimal values.","PeriodicalId":19221,"journal":{"name":"News of Pharmacy","volume":"46 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81128749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Velya, O. Ruban, I. Kovalevska, M. Khalavka, O. Gerush
Aim. To substantiate the optimal composition of the base of the semisolid dosage form with the thick extract of feverfew (Tanacetum parthebased on structural-mechanical, textural, adhesive and microscopic studies. Materials and methods. The study objects were samples of a semisolid dosage form with the thick extract of feverfew with various gelling agents and the reference drug “Irikar”. In the course of the research, a complex of structural-mechanical methods and the microscopic analysis of the samples were used. Results and discussion. The studies of structural-mechanical properties have determined the regularities in the rheological characteristics of the experimental samples made using different gelling agents. It has been found that the rheogram of the emulgel sample has a smooth transition from the elastic deformation region to the elastic-plastic one, which may indicate a more homogeneous distribution of the sample components. It has been also determined that the sample corresponds in the textural properties to the refenium) rence drug – “Irikar” cream. The structural-mechanical studies have substantiated the rational content of the gelling agent in the emulgel composition – 3 %. The results of rheological studies have been confirmed by the data of the microscopic studies. Conclusions. The data obtained are the basis for further pharmaceutical development of a drug with the thick extract of Tanacetum parthenium for the treatment of diseases of the musculoskeletal system.
{"title":"The substantiation of the composition of a soft medicine for the therapy of inflammatory diseases of the musculoskeletal system","authors":"M. Velya, O. Ruban, I. Kovalevska, M. Khalavka, O. Gerush","doi":"10.24959/nphj.23.110","DOIUrl":"https://doi.org/10.24959/nphj.23.110","url":null,"abstract":"Aim. To substantiate the optimal composition of the base of the semisolid dosage form with the thick extract of feverfew (Tanacetum parthebased on structural-mechanical, textural, adhesive and microscopic studies.\u0000Materials and methods. The study objects were samples of a semisolid dosage form with the thick extract of feverfew with various gelling agents and the reference drug “Irikar”. In the course of the research, a complex of structural-mechanical methods and the microscopic analysis of the samples were used.\u0000Results and discussion. The studies of structural-mechanical properties have determined the regularities in the rheological characteristics of the experimental samples made using different gelling agents. It has been found that the rheogram of the emulgel sample has a smooth transition from the elastic deformation region to the elastic-plastic one, which may indicate a more homogeneous distribution of the sample components. It has been also determined that the sample corresponds in the textural properties to the refenium) rence drug – “Irikar” cream. The structural-mechanical studies have substantiated the rational content of the gelling agent in the emulgel composition – 3 %. The results of rheological studies have been confirmed by the data of the microscopic studies.\u0000Conclusions. The data obtained are the basis for further pharmaceutical development of a drug with the thick extract of Tanacetum parthenium for the treatment of diseases of the musculoskeletal system.","PeriodicalId":19221,"journal":{"name":"News of Pharmacy","volume":"10 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81946420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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