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Extracellular microRNAs in Relation to Weight Loss-A Systematic Review and Meta-Analysis. 细胞外微小RNA与减肥的关系——系统综述和荟萃分析。
IF 4.3 Q1 Biochemistry, Genetics and Molecular Biology Pub Date : 2023-09-14 DOI: 10.3390/ncrna9050053
Camilla H B Veie, Isabella M T Nielsen, Nanna L S Frisk, Louise T Dalgaard

Obesity is an important risk factor for cardiovascular disease and type 2 diabetes mellitus. Even a modest weight loss of 5-15% improves metabolic health, but circulating markers to indicate weight loss efficiency are lacking. MicroRNAs, small non-coding post-transcriptional regulators of gene expression, are secreted from tissues into the circulation and may be potential biomarkers for metabolic health. However, it is not known which specific microRNA species are reproducibly changed in levels by weight loss. In this study, we performed a systematic review and meta-analysis to investigate the microRNAs associated with weight loss by comparing baseline to follow-up levels following intervention-driven weight loss. This systematic review was performed according to the PRISMA guidelines with searches in PubMed and SCOPUS. The primary search resulted in a total of 697 articles, which were screened according to the prior established inclusion and exclusion criteria. Following the screening of articles, the review was based on the inclusion of 27 full-text articles, which were evaluated for quality and the risk of bias. We performed systematic data extraction, whereafter the relative values for miRNAs were calculated. A meta-analysis was performed for the miRNA species investigated in three or more studies: miR-26a, miR-126, and miR-223 were overall significantly increased following weight loss, while miR-142 was significantly decreased after weight loss. miR-221, miR-140, miR-122, and miR-146 were not significantly changed by intervention-driven weight loss. These results indicate that few miRNAs are significantly changed during weight loss.

肥胖是心血管疾病和2型糖尿病的重要危险因素。即使是5-15%的适度减肥也能改善代谢健康,但缺乏表明减肥效率的循环标志物。微小RNA是基因表达的小型非编码转录后调节因子,从组织分泌到循环中,可能是代谢健康的潜在生物标志物。然而,目前尚不清楚哪些特定的微小RNA物种的水平会因体重减轻而发生可复制的变化。在这项研究中,我们进行了一项系统综述和荟萃分析,通过比较干预驱动的减肥后的基线水平和随访水平,研究与减肥相关的微小RNA。本系统综述根据PRISMA指南进行,检索PubMed和SCOPUS。初步搜索共产生697篇文章,根据先前确定的纳入和排除标准进行筛选。在对文章进行筛选后,审查的基础是纳入27篇全文文章,对这些文章的质量和偏倚风险进行了评估。我们进行了系统的数据提取,然后计算miRNA的相对值。对三项或多项研究中研究的miRNA物种进行了荟萃分析:miR-26a、miR-126和miR-223在减肥后总体显著增加,而miR-142在减肥后显著减少。miR-221、miR-140、miR-122和miR-146在干预驱动的减肥中没有显著变化。这些结果表明,在减肥过程中,很少有miRNA发生显著变化。
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引用次数: 0
The Potential microRNA Prognostic Signature in HNSCCs: A Systematic Review. HNSCCs潜在的微小RNA预后特征:系统综述。
IF 4.3 Q1 Biochemistry, Genetics and Molecular Biology Pub Date : 2023-09-14 DOI: 10.3390/ncrna9050054
Mario Dioguardi, Francesca Spirito, Giovanna Iacovelli, Diego Sovereto, Enrica Laneve, Luigi Laino, Giorgia Apollonia Caloro, Ari Qadir Nabi, Andrea Ballini, Lorenzo Lo Muzio, Giuseppe Troiano

Head and neck squamous cell carcinomas (HNSCCs) are often diagnosed at advanced stages, incurring significant high mortality and morbidity. Several microRNAs (miRs) have been identified as pivotal players in the onset and advancement of HNSCCs, operating as either oncogenes or tumor suppressors. Distinctive miR patterns identified in tumor samples, as well as in serum, plasma, or saliva, from patients have significant clinical potential for use in the diagnosis and prognosis of HNSCCs and as potential therapeutic targets. The aim of this study was to identify previous systematic reviews with meta-analysis data and clinical trials that showed the most promising miRs in HNSCCs, enclosing them into a biomolecular signature to test the prognostic value on a cohort of HNSCC patients according to The Cancer Genome Atlas (TCGA). Three electronic databases (PubMed, Scopus, and Science Direct) and one registry (the Cochrane Library) were investigated, and a combination of keywords such as "signature microRNA OR miR" AND "HNSCC OR LSCC OR OSCC OR oral cancer" were searched. In total, 15 systematic literature reviews and 76 prognostic clinical reports were identified for the study design and inclusion process. All survival index data were extracted, and the three miRs (miR-21, miR-155, and miR-375) most investigated and presenting the largest number of patients included in the studies were selected in a molecular biosignature. The difference between high and low tissue expression levels of miR-21, miR-155, and miR-375 for OS had an HR = 1.28, with 95% CI: [0.95, 1.72]. In conclusion, the current evidence suggests that miRNAs have potential prognostic value to serve as screening tools for clinical practice in HNSCC follow-up and treatment. Further large-scale cohort studies focusing on these miRNAs are recommended to verify the clinical utility of these markers individually and/or in combination.

头颈部鳞状细胞癌(HNSCC)通常在晚期被诊断,导致显著的高死亡率和发病率。一些微小RNA(miR)已被确定为HNSCCs发生和发展的关键参与者,作为致癌基因或肿瘤抑制剂发挥作用。在患者的肿瘤样本以及血清、血浆或唾液中发现的独特miR模式在HNSCC的诊断和预后以及作为潜在的治疗靶点方面具有重要的临床潜力。本研究的目的是根据癌症基因组图谱(TCGA),通过荟萃分析数据和临床试验确定先前的系统综述,这些综述显示了HNSCC中最有前途的miR,将其封闭在生物分子标记中,以测试HNSCC患者队列的预后价值。研究了三个电子数据库(PubMed、Scopus和Science Direct)和一个注册表(Cochrane图书馆),并搜索了“特征性微小RNA或miR”和“HNSCC或LSCC或OSCC或口腔癌症”等关键词组合。研究设计和纳入过程总共确定了15篇系统文献综述和76份预后临床报告。提取所有生存指数数据,并在分子生物学信号中选择研究最多、患者人数最多的三种miR(miR-21、miR-155和miR-375)。OS的miR-21、miR-155和miR-375的高和低组织表达水平之间的差异HR=1.28,95%CI:[0.95,1.72]。总之,目前的证据表明,miRNA具有潜在的预后价值,可作为HNSCC随访和治疗的临床实践筛选工具。建议对这些miRNA进行进一步的大规模队列研究,以验证这些标志物单独和/或组合的临床效用。
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引用次数: 0
Inverse-Folding Design of Yeast Telomerase RNA Increases Activity In Vitro. 酵母端粒酶RNA的反向折叠设计可提高体外活性。
IF 4.3 Q1 Biochemistry, Genetics and Molecular Biology Pub Date : 2023-08-28 DOI: 10.3390/ncrna9050051
Kevin J Lebo, David C Zappulla

Saccharomyces cerevisiae telomerase RNA, TLC1, is an 1157 nt non-coding RNA that functions as both a template for DNA synthesis and a flexible scaffold for telomerase RNP holoenzyme protein subunits. The tractable budding yeast system has provided landmark discoveries about telomere biology in vivo, but yeast telomerase research has been hampered by the fact that the large TLC1 RNA subunit does not support robust telomerase activity in vitro. In contrast, 155-500 nt miniaturized TLC1 alleles comprising the catalytic core domain and lacking the RNA's long arms do reconstitute robust activity. We hypothesized that full-length TLC1 is prone to misfolding in vitro. To create a full-length yeast telomerase RNA, predicted to fold into its biologically relevant structure, we took an inverse RNA-folding approach, changing 59 nucleotides predicted to increase the energetic favorability of folding into the modeled native structure based on the p-num feature of Mfold software. The sequence changes lowered the predicted ∆G of this "determined-arm" allele, DA-TLC1, by 61 kcal/mol (-19%) compared to wild-type. We tested DA-TLC1 for reconstituted activity and found it to be ~5-fold more robust than wild-type TLC1, suggesting that the inverse-folding design indeed improved folding in vitro into a catalytically active conformation. We also tested if DA-TLC1 functions in vivo, discovering that it complements a tlc1∆ strain, allowing cells to avoid senescence and maintain telomeres of nearly wild-type length. However, all inverse-designed RNAs that we tested had reduced abundance in vivo. In particular, inverse-designing nearly all of the Ku arm caused a profound reduction in telomerase RNA abundance in the cell and very short telomeres. Overall, these results show that the inverse design of S. cerevisiae telomerase RNA increases activity in vitro, while reducing abundance in vivo. This study provides a biochemically and biologically tested approach to inverse-design RNAs using Mfold that could be useful for controlling RNA structure in basic research and biomedicine.

酿酒酵母端粒酶RNA,TLC1,是一种1157nt的非编码RNA,既是DNA合成的模板,也是端粒酶RNP全酶蛋白亚基的柔性支架。易于处理的出芽酵母系统为体内端粒生物学提供了里程碑式的发现,但酵母端粒酶研究受到了阻碍,因为大的TLC1 RNA亚基在体外不支持强大的端粒酶活性。相反,155-500个包含催化核心结构域且缺乏RNA长臂的小型化TLC1等位基因确实重建了强大的活性。我们假设全长TLC1在体外容易发生错误折叠。为了创建全长酵母端粒酶RNA,预测其折叠成其生物学相关结构,我们采用了反向RNA折叠方法,根据Mfold软件的p-num特征,改变59个预测的核苷酸,以增加折叠成模拟天然结构的能量偏好。与野生型相比,序列变化使该“确定臂”等位基因DA-TLC1的预测∆G降低了61 kcal/mol(-19%)。我们测试了DA-TLC1的重组活性,发现它比野生型TLC1强约5倍,这表明反向折叠设计确实改善了体外折叠成催化活性构象的能力。我们还测试了DA-TLC1是否在体内发挥作用,发现它与TLC1∆菌株互补,使细胞避免衰老,并保持接近野生型长度的端粒。然而,我们测试的所有反向设计的RNA在体内的丰度都有所降低。特别是,对几乎所有Ku臂进行逆向设计,导致细胞中端粒酶RNA丰度大幅降低,端粒极短。总之,这些结果表明,酿酒酵母端粒酶RNA的反向设计在体外增加了活性,而在体内降低了丰度。这项研究提供了一种使用Mfold反向设计RNA的生物化学和生物学测试方法,可用于基础研究和生物医学中控制RNA结构。
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引用次数: 0
Non-Coding RNAs: Foes or Friends for Targeting Tumor Microenvironment. 非编码RNA:靶向肿瘤微环境的朋友或朋友。
IF 4.3 Q1 Biochemistry, Genetics and Molecular Biology Pub Date : 2023-08-28 DOI: 10.3390/ncrna9050052
Anna Szymanowska, Cristian Rodriguez-Aguayo, Gabriel Lopez-Berestein, Paola Amero

Non-coding RNAs (ncRNAs) are a group of molecules critical for cell development and growth regulation. They are key regulators of important cellular pathways in the tumor microenvironment. To analyze ncRNAs in the tumor microenvironment, the use of RNA sequencing technology has revolutionized the field. The advancement of this technique has broadened our understanding of the molecular biology of cancer, presenting abundant possibilities for the exploration of novel biomarkers for cancer treatment. In this review, we will summarize recent achievements in understanding the complex role of ncRNA in the tumor microenvironment, we will report the latest studies on the tumor microenvironment using RNA sequencing, and we will discuss the potential use of ncRNAs as therapeutics for the treatment of cancer.

非编码RNA(ncRNA)是一组对细胞发育和生长调控至关重要的分子。它们是肿瘤微环境中重要细胞通路的关键调节因子。为了分析肿瘤微环境中的ncRNA,RNA测序技术的使用已经彻底改变了该领域。这项技术的进步拓宽了我们对癌症分子生物学的理解,为探索用于癌症治疗的新型生物标志物提供了丰富的可能性。在这篇综述中,我们将总结最近在理解ncRNA在肿瘤微环境中的复杂作用方面取得的成就,我们将报道使用RNA测序对肿瘤微环境的最新研究,并讨论ncRNA作为治疗癌症的潜在用途。
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引用次数: 1
Systematic Analysis of Long Non-Coding RNAs in Inflammasome Activation in Monocytes/Macrophages. 单核细胞/巨噬细胞炎症小体激活中长非编码RNA的系统分析。
IF 4.3 Q1 Biochemistry, Genetics and Molecular Biology Pub Date : 2023-08-25 DOI: 10.3390/ncrna9050050
Na Qian, Rebecca Distefano, Mirolyuba Ilieva, Jens Hedelund Madsen, Sarah Rennie, Shizuka Uchida

The NLRP3 inflammasome plays a pivotal role in regulating inflammation and immune responses. Its activation can lead to an inflammatory response and pyroptotic cell death. This is beneficial in the case of infections, but excessive activation can lead to chronic inflammation and tissue damage. Moreover, while most of the mammalian genome is transcribed as RNAs, only a small fraction codes for proteins. Among non-protein-coding RNAs, long non-coding RNAs (lncRNAs) have been shown to play key roles in regulating gene expression and cellular processes. They interact with DNA, RNAs, and proteins, and their dysregulation can provide insights into disease mechanisms, including NLRP3 inflammasome activation. Here, we systematically analyzed previously published RNA sequencing (RNA-seq) data of NLRP3 inflammasome activation in monocytes/macrophages to uncover inflammasome-regulated lncRNA genes. To uncover the functional importance of inflammasome-regulated lncRNA genes, one inflammasome-regulated lncRNA, ENSG00000273124, was knocked down in an in vitro model of macrophage polarization. The results indicate that silencing of ENSG00000273124 resulted in the up-regulation tumor necrosis factor (TNF), suggesting that this lncRNA might be involved in pro-inflammatory response in macrophages. To make our analyzed data more accessible, we developed the web database InflammasomeDB.

NLRP3炎症小体在调节炎症和免疫反应中起着关键作用。它的激活可导致炎症反应和焦性细胞死亡。这在感染的情况下是有益的,但过度激活会导致慢性炎症和组织损伤。此外,尽管哺乳动物基因组的大部分被转录为RNA,但只有一小部分编码蛋白质。在非蛋白质编码RNA中,长非编码RNA(lncRNA)已被证明在调节基因表达和细胞过程中发挥关键作用。它们与DNA、RNA和蛋白质相互作用,它们的失调可以深入了解疾病机制,包括NLRP3炎症小体的激活。在这里,我们系统地分析了先前发表的单核细胞/巨噬细胞中NLRP3炎症小体激活的RNA测序(RNA-seq)数据,以揭示炎症小体调节的lncRNA基因。为了揭示炎症小体调节的lncRNA基因的功能重要性,在巨噬细胞极化的体外模型中敲除了一种炎症小体调节lncRNA,ENSG00000273124。结果表明,ENSG00000273124的沉默导致肿瘤坏死因子(TNF)上调,表明该lncRNA可能参与巨噬细胞的促炎反应。为了使我们分析的数据更容易访问,我们开发了网络数据库InflammatomeDB。
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引用次数: 0
Advances and Obstacles in Using CRISPR/Cas9 Technology for Non-Coding RNA Gene Knockout in Human Mesenchymal Stromal Cells. CRISPR/Cas9技术用于人间充质干细胞非编码RNA基因敲除的进展和障碍。
IF 4.3 Q1 Biochemistry, Genetics and Molecular Biology Pub Date : 2023-08-24 DOI: 10.3390/ncrna9050049
Nataliya Basalova, Maria Illarionova, Mariya Skryabina, Maksim Vigovskiy, Anastasia Tolstoluzhinskaya, Alexandra Primak, Elizaveta Chechekhina, Vadim Chechekhin, Maxim Karagyaur, Anastasia Efimenko

Non-coding RNA (ncRNAs) genes have attracted increasing attention in recent years due to their widespread involvement in physiological and pathological processes and regulatory networks. The study of the function and molecular partners of ncRNAs opens up opportunities for the early diagnosis and treatment of previously incurable diseases. However, the classical "loss-of-function" approach in ncRNA function analysis is challenged due to some specific issues. Here, we have studied the potency of two CRISPR/Cas9 variants, wild-type (SpCas9wt) and nickase (SpCas9D10A) programmable nucleases, for the editing of extended DNA sequences in human mesenchymal stromal cells (MSCs). Editing the genes of fibrosis-related hsa-miR-21-5p and hsa-miR-29c-3p, we have shown that a pair of SpCas9D10A molecules can effectively disrupt miRNA genes within the genomes of MSCs. This leads not only to a decrease in the level of knockout miRNA in MSCs and MSC-produced extracellular vesicles, but also to a change in cell physiology and the antifibrotic properties of the cell secretome. These changes correlate well with previously published data for the knockdown of certain miRNAs. The proposed approach can be used to knock out ncRNA genes within the genomes of MSCs or similar cell types in order to study their function in biological processes.

近年来,非编码RNA(ncRNAs)基因因其广泛参与生理和病理过程以及调控网络而引起越来越多的关注。对ncRNA功能和分子伴侣的研究为早期诊断和治疗以前无法治愈的疾病开辟了机会。然而,由于一些特定的问题,ncRNA功能分析中的经典“功能损失”方法受到了挑战。在这里,我们研究了两种CRISPR/Cas9变体,野生型(SpCas9wt)和镍酶(SpCas9 D10A)可编程核酸酶,用于编辑人间充质基质细胞(MSC)中的扩展DNA序列的效力。编辑纤维化相关的hsa-miR-21-5p和hsa-miR-29c-3p的基因,我们已经表明一对SpCas9D10A分子可以有效地破坏MSC基因组中的miRNA基因。这不仅导致MSC和MSC产生的细胞外小泡中敲除miRNA的水平降低,还导致细胞生理学和细胞分泌组抗纤维化特性的改变。这些变化与先前发表的敲低某些miRNA的数据有很好的相关性。所提出的方法可用于敲除MSC或类似细胞类型基因组中的ncRNA基因,以研究其在生物过程中的功能。
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引用次数: 0
Analysis of lncRNAs in Lupinus mutabilis (Tarwi) and Their Potential Role in Drought Response. 突变狼疮lncRNA的分析及其在干旱反应中的潜在作用。
IF 4.3 Q1 Biochemistry, Genetics and Molecular Biology Pub Date : 2023-08-23 DOI: 10.3390/ncrna9050048
Manuel Hidalgo, Cynthia Ramos, Gaston Zolla

Lupinus mutabilis is a legume with high agronomic potential and available transcriptomic data for which lncRNAs have not been studied. Therefore, our objective was to identify, characterize, and validate the drought-responsive lncRNAs in L. mutabilis. To achieve this, we used a multilevel approach based on lncRNA prediction, annotation, subcellular location, thermodynamic characterization, structural conservation, and validation. Thus, 590 lncRNAs were identified by at least two algorithms of lncRNA identification. Annotation with the PLncDB database showed 571 lncRNAs unique to tarwi and 19 lncRNAs with homology in 28 botanical families including Solanaceae (19), Fabaceae (17), Brassicaceae (17), Rutaceae (17), Rosaceae (16), and Malvaceae (16), among others. In total, 12 lncRNAs had homology in more than 40 species. A total of 67% of lncRNAs were located in the cytoplasm and 33% in exosomes. Thermodynamic characterization of S03 showed a stable secondary structure with -105.67 kcal/mol. This structure included three regions, with a multibranch loop containing a hairpin with a SECIS-like element. Evaluation of the structural conservation by CROSSalign revealed partial similarities between L. mutabilis (S03) and S. lycopersicum (Solyc04r022210.1). RT-PCR validation demonstrated that S03 was upregulated in a drought-tolerant accession of L. mutabilis. Finally, these results highlighted the importance of lncRNAs in tarwi improvement under drought conditions.

突变狼疮是一种具有高农艺潜力和可用转录组数据的豆类,其lncRNA尚未研究。因此,我们的目的是鉴定、表征和验证突变乳杆菌中的干旱响应lncRNA。为了实现这一点,我们使用了一种基于lncRNA预测、注释、亚细胞定位、热力学表征、结构守恒和验证的多级方法。因此,通过至少两种lncRNA鉴定算法鉴定了590个lncRNA。PLncDB数据库的注释显示,在28个植物科中,有571个tarwi特有的lncRNA和19个具有同源性的lncRNAs,包括茄科(19)、豆科(17)、十字花科(17),芸香科(17。总共有12个lncRNA在40多个物种中具有同源性。共有67%的lncRNA位于细胞质中,33%位于外泌体中。S03的热力学表征显示出稳定的二级结构,为-105.67kcal/mol。该结构包括三个区域,其中一个多分支环包含一个带有类似SECIS元素的发夹。CROSSalign对结构保守性的评估揭示了突变乳杆菌(S03)和番茄(Solyc04r022210.1)之间的部分相似性。RT-PCR验证表明,S03在突变乳杆菌的耐旱登录中上调。最后,这些结果强调了lncRNA在干旱条件下改善tarwi的重要性。
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引用次数: 0
Investigation into the Role of Long-Non-Coding RNA MIAT in Leukemia. 长链非编码RNA MIAT在白血病中的作用研究。
IF 4.3 Q1 Biochemistry, Genetics and Molecular Biology Pub Date : 2023-08-11 DOI: 10.3390/ncrna9040047
Alessia Ostini, Mirna Mourtada-Maarabouni

Myocardial Infarction Associated Transcript (MIAT) is a nuclear long non-coding RNA (LncRNA) with four different splicing variants. MIAT dysregulation is associated with carcinogenesis, mainly acting as an oncogene regulating cellular growth, invasion, and metastasis. The aim of the current study is to investigate the role of MIAT in the regulation of T and chronic myeloid leukemic cell survival. To this end, MIAT was silenced using MIAT-specific siRNAs in leukemic cell lines, and functional assays were performed thereafter. This investigation also aims to investigate the effects of MIAT silencing on the expression of core genes involved in cancer. Functional studies and gene expression determination confirm that MIAT knockdown not only affects short- and long-term survival and the apoptosis of leukemic cells but also plays a pivotal role in the alteration of key genes involved in cancer, including c-MYC and HIF-1A. Our observations suggest that MIAT could act as an oncogene and it has the potential to be used not only as a reliable biomarker for leukemia, but also be employed for prognostic and therapeutic purposes.

心肌梗死相关转录本(MIAT)是一种核长链非编码RNA (LncRNA),具有四种不同的剪接变体。MIAT失调与癌变有关,主要作为癌基因调节细胞生长、侵袭和转移。本研究的目的是探讨mat在T细胞和慢性髓性白血病细胞存活调控中的作用。为此,在白血病细胞系中使用MIAT特异性sirna沉默MIAT,然后进行功能分析。本研究还旨在探讨MIAT沉默对癌症相关核心基因表达的影响。功能研究和基因表达测定证实,MIAT敲低不仅影响白血病细胞的短期和长期存活及凋亡,还在癌症相关关键基因c-MYC和HIF-1A的改变中起关键作用。我们的观察结果表明,MIAT可以作为一种致癌基因,它不仅有潜力作为白血病的可靠生物标志物,而且还可用于预后和治疗目的。
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引用次数: 0
Structural Modifications and Novel Protein-Binding Sites in Pre-miR-675-Explaining Its Regulatory Mechanism in Carcinogenesis. Pre-miR-675的结构修饰和新型蛋白结合位点--解释其在致癌过程中的调控机制
IF 3.6 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2023-08-10 DOI: 10.3390/ncrna9040045
Abhishek Dey

Pre-miR-675 is a microRNA expressed from the exon 1 of H19 long noncoding RNA, and the atypical expression of pre-miR-675 has been linked with several diseases and disorders including cancer. To execute its function inside the cell, pre-miR-675 is folded into a particular conformation, which aids in its interaction with several other biological molecules. However, the exact folding dynamics of pre-miR-675 and its protein-binding motifs are currently unknown. Moreover, how H19 lncRNA and pre-miR-675 crosstalk and modulate each other's activities is also unclear. The detailed structural analysis of pre-miR-675 in this study determines its earlier unknown conformation and identifies novel protein-binding sites on pre-miR-675, thus making it an excellent therapeutic target against cancer. Co-folding analysis between H19 lncRNA and pre-miR-675 determine structural transformations in pre-miR-675, thus describing the earlier unknown mechanism of interaction between these two molecules. Comprehensively, this study details the conformation of pre-miR-675 and its protein-binding sites and explains its relationship with H19 lncRNA, which can be interpreted to understand the role of pre-miR-675 in the development and progression of tumorigenesis and designing new therapeutics against cancers.

pre-miR-675 是一种从 H19 长非编码 RNA 第 1 外显子表达的 microRNA,pre-miR-675 的非典型表达与包括癌症在内的多种疾病和失调有关。为了在细胞内执行其功能,pre-miR-675 折叠成一个特定的构象,这有助于它与其他几种生物分子的相互作用。然而,pre-miR-675 的确切折叠动态及其蛋白质结合基团目前尚不清楚。此外,H19 lncRNA 和 pre-miR-675 如何相互影响和调节彼此的活性也不清楚。本研究对pre-miR-675进行了详细的结构分析,确定了其早期未知的构象,并发现了pre-miR-675上新的蛋白结合位点,从而使其成为癌症的一个极佳治疗靶点。H19 lncRNA与pre-miR-675之间的共折叠分析确定了pre-miR-675的结构转变,从而描述了这两个分子之间早先未知的相互作用机制。总之,本研究详细阐述了pre-miR-675的构象及其蛋白结合位点,并解释了其与H19 lncRNA的关系,可用于理解pre-miR-675在肿瘤发生和发展过程中的作用,并设计新的抗癌疗法。
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引用次数: 0
Methods and Tools in RNA Biology. RNA生物学的方法和工具。
IF 4.3 Q1 Biochemistry, Genetics and Molecular Biology Pub Date : 2023-08-10 DOI: 10.3390/ncrna9040046
Mirolyuba Ilieva, Shizuka Uchida

Breakthroughs in innovative techniques and instruments have driven the exploration of non-coding RNAs (ncRNAs), including microRNAs (miRNAs) and long non-coding RNAs (lncRNAs) [...].

创新技术和仪器的突破推动了对非编码rna (ncRNAs)的探索,包括微rna (miRNAs)和长链非编码rna (lncRNAs)[…]。
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引用次数: 0
期刊
Non-Coding RNA
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