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Impacts of MicroRNA-483 on Human Diseases. MicroRNA-483对人类疾病的影响。
IF 3.6 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2023-06-28 DOI: 10.3390/ncrna9040037
Katy Matson, Aaron Macleod, Nirali Mehta, Ellie Sempek, Xiaoqing Tang

MicroRNAs (miRNAs) are short non-coding RNA molecules that regulate gene expression by targeting specific messenger RNAs (mRNAs) in distinct cell types. This review provides a com-prehensive overview of the current understanding regarding the involvement of miR-483-5p and miR-483-3p in various physiological and pathological processes. Downregulation of miR-483-5p has been linked to numerous diseases, including type 2 diabetes, fatty liver disease, diabetic nephropathy, and neurological injury. Accumulating evidence indicates that miR-483-5p plays a crucial protective role in preserving cell function and viability by targeting specific transcripts. Notably, elevated levels of miR-483-5p in the bloodstream strongly correlate with metabolic risk factors and serve as promising diagnostic markers. Consequently, miR-483-5p represents an appealing biomarker for predicting the risk of developing diabetes and cardiovascular diseases and holds potential as a therapeutic target for intervention strategies. Conversely, miR-483-3p exhibits significant upregulation in diabetes and cardiovascular diseases and has been shown to induce cellular apoptosis and lipotoxicity across various cell types. However, some discrepancies regarding its precise function have been reported, underscoring the need for further investigation in this area.

微小RNA(miRNA)是一种短的非编码RNA分子,通过靶向不同细胞类型中的特异性信使RNA(mRNA)来调节基因表达。这篇综述对miR-483-5p和miR-483-3p参与各种生理和病理过程的当前理解进行了全面综述。miR-483-5p的下调与许多疾病有关,包括2型糖尿病、脂肪肝、糖尿病肾病和神经损伤。越来越多的证据表明,miR-483-5p通过靶向特定转录物,在保持细胞功能和活力方面发挥着至关重要的保护作用。值得注意的是,血液中miR-483-5p水平的升高与代谢风险因素密切相关,可作为有前景的诊断标志物。因此,miR-483-5p是预测糖尿病和心血管疾病风险的一种有吸引力的生物标志物,有可能成为干预策略的治疗靶点。相反,miR-483-3p在糖尿病和心血管疾病中表现出显著的上调,并已被证明可诱导各种细胞类型的细胞凋亡和脂毒性。然而,据报告,在其确切功能方面存在一些差异,这突出表明需要在这一领域进行进一步调查。
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引用次数: 0
Small Nucleolar (Sno)RNA: Therapy Lays in Translation. 小核仁RNA:治疗在于翻译。
IF 4.3 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2023-06-08 DOI: 10.3390/ncrna9030035
Ofri Rabany, Daphna Nachmani

The ribosome is one of the largest complexes in the cell. Adding to its complexity are more than 200 RNA modification sites present on ribosomal RNAs (rRNAs) in a single human ribosome. These modifications occur in functionally important regions of the rRNA molecule, and they are vital for ribosome function and proper gene expression. Until recent technological advancements, the study of rRNA modifications and their profiles has been extremely laborious, leaving many questions unanswered. Small nucleolar RNAs (snoRNAs) are non-coding RNAs that facilitate and dictate the specificity of rRNA modification deposition, making them an attractive target for ribosome modulation. Here, we propose that through the mapping of rRNA modification profiles, we can identify cell-specific modifications with high therapeutic potential. We also describe the challenges of achieving the targeting specificity needed to implement snoRNAs as therapeutic targets in cancers.

核糖体是细胞中最大的复合体之一。在单个人类核糖体的核糖体RNA (RNAs)上存在超过200个RNA修饰位点,使其更加复杂。这些修饰发生在rRNA分子的重要功能区域,它们对核糖体功能和正确的基因表达至关重要。在最近的技术进步之前,对rRNA修饰及其谱的研究一直非常费力,留下了许多未解之谜。小核仁rna (Small nucleolar rna, snoRNAs)是一种非编码rna,它促进并决定了rRNA修饰沉积的特异性,使其成为核糖体调节的一个有吸引力的靶标。在这里,我们提出,通过绘制rRNA修饰谱,我们可以识别具有高治疗潜力的细胞特异性修饰。我们还描述了实现snorna作为癌症治疗靶点所需的靶向特异性所面临的挑战。
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引用次数: 1
A Review of IsomiRs in Colorectal Cancer. 大肠癌中的 IsomiRs 综述。
IF 3.6 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2023-06-07 DOI: 10.3390/ncrna9030034
Molly A Lausten, Bruce M Boman

As advancements in sequencing technology rapidly continue to develop, a new classification of microRNAs has occurred with the discovery of isomiRs, which are relatively common microRNAs with sequence variations compared to their established template microRNAs. This review article seeks to compile all known information about isomiRs in colorectal cancer (CRC), which has not, to our knowledge, been gathered previously to any great extent. A brief overview is given of the history of microRNAs, their implications in colon cancer, the canonical pathway of biogenesis and isomiR classification. This is followed by a comprehensive review of the literature that is available on microRNA isoforms in CRC. The information on isomiRs presented herein shows that isomiRs hold great promise for translation into new diagnostics and therapeutics in clinical medicine.

随着测序技术的飞速发展,microRNAs 的新分类也随之出现,isomiRs 被发现,它们是相对常见的 microRNAs,与其既定的模板 microRNAs 相比,序列存在变异。这篇综述文章试图汇集所有已知的有关结直肠癌(CRC)中isomiRs的信息,据我们所知,这些信息以前从未在很大程度上收集过。文章简要概述了 microRNA 的历史、其在结肠癌中的影响、生物发生的典型途径以及 isomiR 的分类。随后全面回顾了有关结肠癌中 microRNA 同工酶的现有文献。本文介绍的异构体信息表明,异构体很有希望转化为临床医学中的新诊断和治疗方法。
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引用次数: 0
A microRNA Arising from the Negative Strand of SARS-CoV-2 Genome Targets FOS to Reduce AP-1 Activity. 由SARS-CoV-2基因组负链产生的microRNA靶向FOS降低AP-1活性
IF 4.3 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2023-05-23 DOI: 10.3390/ncrna9030033
Francesco Greco, Elisa Lorefice, Claudia Carissimi, Ilaria Laudadio, Fabiola Ciccosanti, Martina Di Rienzo, Francesca Colavita, Silvia Meschi, Fabrizio Maggi, Gian Maria Fimia, Valerio Fulci

Virus-encoded microRNAs were first reported in the Epstein-Barr virus in 2004. Subsequently, a few hundred viral miRNAs have been identified, mainly in DNA viruses belonging to the herpesviridae family. To date, only 30 viral miRNAs encoded by RNA viruses are reported by miRBase. Since the outbreak of the SARS-CoV-2 pandemic, several studies have predicted and, in some cases, experimentally validated miRNAs originating from the positive strand of the SARS-CoV-2 genome. By integrating NGS data analysis and qRT-PCR approaches, we found that SARS-CoV-2 also encodes for a viral miRNA arising from the minus (antisense) strand of the viral genome, in the region encoding for ORF1ab, herein referred to as SARS-CoV-2-miR-AS1. Our data show that the expression of this microRNA increases in a time course analysis of SARS-CoV-2 infected cells. Furthermore, enoxacin treatment enhances the accumulation of the mature SARS-CoV-2-miR-AS1 in SARS-CoV-2 infected cells, arguing for a Dicer-dependent processing of this small RNA. In silico analysis suggests that SARS-CoV-2-miR-AS1 targets a set of genes which are translationally repressed during SARS-CoV-2 infection. We experimentally validated that SARS-CoV-2-miR-AS1 targets FOS, thus repressing the AP-1 transcription factor activity in human cells.

2004年,在爱泼斯坦-巴尔病毒中首次报道了病毒编码的microrna。随后,几百个病毒mirna被鉴定出来,主要是在属于疱疹病毒科的DNA病毒中。迄今为止,miRBase仅报道了30种由RNA病毒编码的病毒mirna。自SARS-CoV-2大流行爆发以来,已有几项研究预测并在某些情况下通过实验验证了源自SARS-CoV-2基因组阳性链的mirna。通过整合NGS数据分析和qRT-PCR方法,我们发现SARS-CoV-2也编码来自病毒基因组负链(反义)的病毒miRNA,在编码ORF1ab的区域,这里称为SARS-CoV-2- mir - as1。我们的数据显示,在SARS-CoV-2感染细胞的时间过程分析中,这种microRNA的表达增加。此外,依诺沙星治疗增强了成熟的SARS-CoV-2- mir - as1在SARS-CoV-2感染细胞中的积累,证明这种小RNA的加工依赖于dicer。计算机分析表明,SARS-CoV-2- mir - as1靶向一组在SARS-CoV-2感染期间翻译抑制的基因。我们通过实验验证了SARS-CoV-2-miR-AS1靶向FOS,从而抑制人类细胞中AP-1转录因子的活性。
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引用次数: 0
Inverse Impact of Cancer Drugs on Circular and Linear RNAs in Breast Cancer Cell Lines. 抗癌药物对乳腺癌细胞系环状和线状rna的反向影响
IF 4.3 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2023-05-19 DOI: 10.3390/ncrna9030032
Anna Terrazzan, Francesca Crudele, Fabio Corrà, Pietro Ancona, Jeffrey Palatini, Nicoletta Bianchi, Stefano Volinia

Altered expression of circular RNAs (circRNAs) has previously been investigated in breast cancer. However, little is known about the effects of drugs on their regulation and relationship with the cognate linear transcript (linRNA). We analyzed the dysregulation of both 12 cancer-related circRNAs and their linRNAs in two breast cancer cell lines undergoing various treatments. We selected 14 well-known anticancer agents affecting different cellular pathways and examined their impact. Upon drug exposure circRNA/linRNA expression ratios increased, as a result of the downregulation of linRNA and upregulation of circRNA within the same gene. In this study, we highlighted the relevance of identifying the drug-regulated circ/linRNAs according to their oncogenic or anticancer role. Interestingly, VRK1 and MAN1A2 were increased by several drugs in both cell lines. However, they display opposite effects, circ/linVRK1 favors apoptosis whereas circ/linMAN1A2 stimulates cell migration, and only XL765 did not alter the ratio of other dangerous circ/linRNAs in MCF-7. In MDA-MB-231 cells, AMG511 and GSK1070916 decreased circGFRA1, as a good response to drugs. Furthermore, some circRNAs might be associated with specific mutated pathways, such as the PI3K/AKT in MCF-7 cells with circ/linHIPK3 correlating to cancer progression and drug-resistance, or NHEJ DNA repair pathway in TP-53 mutated MDA-MB-231 cells.

环状rna (circRNAs)的表达改变在乳腺癌中已经被研究过。然而,对于药物对其调控的影响以及与同源线性转录物(linRNA)的关系知之甚少。我们分析了两种接受不同治疗的乳腺癌细胞系中12种与癌症相关的环状rna及其linrna的失调。我们选择了14种已知的抗癌药物影响不同的细胞途径,并检查了它们的影响。药物暴露后,由于同一基因内的linRNA下调和circRNA上调,circRNA/linRNA的表达比增加。在这项研究中,我们强调了根据其致癌或抗癌作用鉴定药物调控的circ/ linrna的相关性。有趣的是,VRK1和MAN1A2在两种细胞系中被几种药物增加。然而,它们表现出相反的作用,circ/linVRK1促进细胞凋亡,而circ/linMAN1A2刺激细胞迁移,只有XL765没有改变MCF-7中其他危险circ/linRNAs的比例。在MDA-MB-231细胞中,AMG511和GSK1070916降低了circGFRA1,这是对药物的良好反应。此外,一些circrna可能与特定的突变途径相关,如MCF-7细胞中的PI3K/AKT, circ/linHIPK3与癌症进展和耐药相关,或TP-53突变的MDA-MB-231细胞中的NHEJ DNA修复途径。
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引用次数: 0
Genetic Deletion of the LINC00520 Homolog in Mouse Aggravates Angiotensin II-Induced Hypertension. 小鼠 LINC00520 同源物的基因缺失会加重血管紧张素 II 诱导的高血压。
IF 3.6 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2023-05-15 DOI: 10.3390/ncrna9030031
Xiaofang Tang, Chih-Hung Lai, Naseeb K Malhi, Rahuljeet Chadha, Yingjun Luo, Xuejing Liu, Dongqiang Yuan, Alonso Tapia, Maryam Abdollahi, Guangyu Zhang, Riccardo Calandrelli, Yan-Ting Shiu, Zhao V Wang, June-Wha Rhee, Sheng Zhong, Rama Natarajan, Zhen Bouman Chen

(1) Background: Hypertension is a complex, multifactorial disease that is caused by genetic and environmental factors. Apart from genetic predisposition, the mechanisms involved in this disease have yet to be fully understood. We previously reported that LEENE (lncRNA enhancing endothelial nitric oxide expression, transcribed from LINC00520 in the human genome) regulates endothelial cell (EC) function by promoting the expression of endothelial nitric oxide synthase (eNOS) and vascular growth factor receptor 2 (VEGFR2). Mice with genetic deletion of the LEENE/LINC00520 homologous region exhibited impaired angiogenesis and tissue regeneration in a diabetic hindlimb ischemia model. However, the role of LEENE in blood pressure regulation is unknown. (2) Methods: We subjected mice with genetic ablation of leene and wild-type littermates to Angiotensin II (AngII) and monitored their blood pressure and examined their hearts and kidneys. We used RNA-sequencing to identify potential leene-regulated molecular pathways in ECs that contributed to the observed phenotype. We further performed in vitro experiments with murine and human ECs and ex vivo experiments with murine aortic rings to validate the select mechanism. (3) Results: We identified an exacerbated hypertensive phenotype of leene-KO mice in the AngII model, evidenced by higher systolic and diastolic blood pressure. At the organ level, we observed aggravated hypertrophy and fibrosis in the heart and kidney. Moreover, the overexpression of human LEENE RNA, in part, restored the signaling pathways impaired by leene deletion in murine ECs. Additionally, Axitinib, a tyrosine kinase inhibitor that selectively inhibits VEGFR suppresses LEENE in human ECs. (4) Conclusions: Our study suggests LEENE as a potential regulator in blood pressure control, possibly through its function in ECs.

(1) 背景:高血压是一种复杂的多因素疾病,由遗传和环境因素引起。除遗传易感性外,该病的发病机制尚不完全清楚。我们以前曾报道,LEENE(增强内皮一氧化氮表达的lncRNA,转录自人类基因组中的LINC00520)通过促进内皮一氧化氮合酶(eNOS)和血管生长因子受体2(VEGFR2)的表达来调节内皮细胞(EC)的功能。在糖尿病后肢缺血模型中,基因缺失LEENE/LINC00520同源区的小鼠表现出血管生成和组织再生功能受损。然而,LEENE在血压调节中的作用尚不清楚。(2)方法:我们让leene基因消减的小鼠和野生型小鼠接受血管紧张素II(AngII)治疗,监测它们的血压并检查它们的心脏和肾脏。我们使用 RNA 测序技术鉴定了导致观察到的表型的心血管细胞中潜在的leene调控分子通路。我们进一步用小鼠和人类心血管细胞进行了体外实验,并用小鼠主动脉环进行了体内外实验,以验证选择机制。(3) 结果:我们发现在 AngII 模型中,leene-KO 小鼠的高血压表型加剧,表现为收缩压和舒张压升高。在器官层面,我们观察到心脏和肾脏肥大和纤维化加剧。此外,人 LEENE RNA 的过表达在一定程度上恢复了小鼠 EC 中因 LEENE 缺失而受损的信号通路。此外,选择性抑制血管内皮生长因子受体的酪氨酸激酶抑制剂 Axitinib 可抑制人 EC 中的 LEENE。(4)结论:我们的研究表明,LEENE 是血压控制的潜在调节因子,可能是通过其在 ECs 中的功能实现的。
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引用次数: 0
T2DB: A Web Database for Long Non-Coding RNA Genes in Type II Diabetes. T2DB:II型糖尿病中长非编码RNA基因的网络数据库。
IF 4.3 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2023-05-08 DOI: 10.3390/ncrna9030030
Rebecca Distefano, Mirolyuba Ilieva, Jens Hedelund Madsen, Hideshi Ishii, Masanori Aikawa, Sarah Rennie, Shizuka Uchida

Type II diabetes (T2D) is a growing health problem worldwide due to increased levels of obesity and can lead to other life-threatening diseases, such as cardiovascular and kidney diseases. As the number of individuals diagnosed with T2D rises, there is an urgent need to understand the pathogenesis of the disease in order to prevent further harm to the body caused by elevated blood glucose levels. Recent advances in long non-coding RNA (lncRNA) research may provide insights into the pathogenesis of T2D. Although lncRNAs can be readily detected in RNA sequencing (RNA-seq) data, most published datasets of T2D patients compared to healthy donors focus only on protein-coding genes, leaving lncRNAs to be undiscovered and understudied. To address this knowledge gap, we performed a secondary analysis of published RNA-seq data of T2D patients and of patients with related health complications to systematically analyze the expression changes of lncRNA genes in relation to the protein-coding genes. Since immune cells play important roles in T2D, we conducted loss-of-function experiments to provide functional data on the T2D-related lncRNA USP30-AS1, using an in vitro model of pro-inflammatory macrophage activation. To facilitate lncRNA research in T2D, we developed a web application, T2DB, to provide a one-stop-shop for expression profiling of protein-coding and lncRNA genes in T2D patients compared to healthy donors or subjects without T2D.

由于肥胖水平的增加,II型糖尿病(T2D)在全球范围内是一个日益严重的健康问题,并可能导致其他危及生命的疾病,如心血管和肾脏疾病。随着被诊断为T2D的人数增加,迫切需要了解该疾病的发病机制,以防止血糖水平升高对身体造成进一步伤害。长期非编码RNA(lncRNA)研究的最新进展可能为T2D的发病机制提供见解。尽管lncRNA可以很容易地在RNA测序(RNA-seq)数据中检测到,但与健康供体相比,大多数已发表的T2D患者数据集只关注蛋白质编码基因,这使得lncRNA尚未被发现和研究不足。为了解决这一知识差距,我们对T2D患者和有相关健康并发症的患者的已发表RNA-seq数据进行了二次分析,以系统分析lncRNA基因与蛋白质编码基因的表达变化。由于免疫细胞在T2D中发挥重要作用,我们使用促炎巨噬细胞激活的体外模型进行了功能丧失实验,以提供T2D相关lncRNA USP30-AS1的功能数据。为了促进T2D中lncRNA的研究,我们开发了一个名为T2DB的网络应用程序,与健康供体或无T2D的受试者相比,为T2D患者的蛋白质编码和lncRNA基因的表达谱分析提供了一站式服务。
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引用次数: 0
The Non-Coding RNA Journal Club: Highlights on Recent Papers-12. 非编码RNA杂志俱乐部:最近论文的亮点-12。
IF 4.3 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2023-04-18 DOI: 10.3390/ncrna9020028
Patrick K T Shiu, Mirolyuba Ilieva, Anja Holm, Shizuka Uchida, Johanna K DiStefano, Agnieszka Bronisz, Ling Yang, Yoh Asahi, Ajay Goel, Liuqing Yang, Ashok Nuthanakanti, Alexander Serganov, Suresh K Alahari, Chunru Lin, Barbara Pardini, Alessio Naccarati, Jing Jin, Beshoy Armanios, Xiao-Bo Zhong, Nikolaos Sideris, Salih Bayraktar, Leandro Castellano, André P Gerber, He Lin, Simon J Conn, Doha Magdy Mostafa Sleem, Lisa Timmons

We are delighted to share with you our twelfth Journal Club and highlight some of the most interesting papers published recently [...].

我们很高兴与您分享第十二届期刊俱乐部,并重点介绍最近发表的一些最有趣的论文[…]。
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引用次数: 0
The Cystic Fibrosis Transmembrane Conductance Regulator Gene (CFTR) Is under Post-Transcriptional Control of microRNAs: Analysis of the Effects of agomiRNAs Mimicking miR-145-5p, miR-101-3p, and miR-335-5p. 囊性纤维化跨膜传导调节基因(CFTR)受microRNAs转录后控制:模拟miR-145-5p、miR-101-3p和miR-335-5p的agomiRNAs的作用分析。
IF 4.3 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2023-04-18 DOI: 10.3390/ncrna9020029
Chiara Papi, Jessica Gasparello, Matteo Zurlo, Lucia Carmela Cosenza, Roberto Gambari, Alessia Finotti

(1) Background: MicroRNAs are involved in the expression of the gene encoding the chloride channel CFTR (Cystic Fibrosis Transmembrane Conductance Regulator); the objective of this short report is to study the effects of the treatment of bronchial epithelial Calu-3 cells with molecules mimicking the activity of pre-miR-145-5p, pre-miR-335-5p, and pre-miR-101-3p, and to discuss possible translational applications of these molecules in pre-clinical studies focusing on the development of protocols of possible interest in therapy; (2) Methods: CFTR mRNA was quantified by Reverse Transcription quantitative Polymerase Chain Reaction (RT-qPCR). The production of the CFTR protein was assessed by Western blotting; (3) Results: The treatment of Calu-3 cells with agomiR-145-5p caused the highest inhibition of CFTR mRNA accumulation and CFTR production; (4) Conclusions: The treatment of target cells with the agomiR pre-miR-145-5p should be considered when CFTR gene expression should be inhibited in pathological conditions, such as polycystic kidney disease (PKD), some types of cancer, cholera, and SARS-CoV-2 infection.

(1)背景:MicroRNAs参与氯离子通道CFTR(囊性纤维化跨膜传导调节因子)编码基因的表达;这篇简短报告的目的是研究用模拟pre-miR-145-5p、pre-miR-335-5p和pre-miR-101-3p活性的分子治疗支气管上皮Calu-3细胞的效果,并讨论这些分子在临床前研究中的可能的转化应用,重点是开发可能感兴趣的治疗方案;(2)方法:采用逆转录定量聚合酶链式反应(RT-qPCR)定量CFTR mRNA。Western blotting检测CFTR蛋白的产生;(3)结果:agomiR-145-5p处理Calu-3细胞对CFTR mRNA积累和CFTR产生的抑制作用最大;(4)结论:在多囊肾病(PKD)、某些类型的癌症、霍乱、SARS-CoV-2感染等病理情况下,当需要抑制CFTR基因表达时,应考虑agomiR pre-miR-145-5p对靶细胞的治疗。
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引用次数: 2
MicroRNAs in Age-Related Proteostasis and Stress Responses. 年龄相关的蛋白质平衡和应激反应中的microrna。
IF 4.3 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2023-04-13 DOI: 10.3390/ncrna9020026
Latika Matai, Frank J Slack

Aging is associated with the accumulation of damaged and misfolded proteins through a decline in the protein homeostasis (proteostasis) machinery, leading to various age-associated protein misfolding diseases such as Huntington's or Parkinson's. The efficiency of cellular stress response pathways also weakens with age, further contributing to the failure to maintain proteostasis. MicroRNAs (miRNAs or miRs) are a class of small, non-coding RNAs (ncRNAs) that bind target messenger RNAs at their 3'UTR, resulting in the post-transcriptional repression of gene expression. From the discovery of aging roles for lin-4 in C. elegans, the role of numerous miRNAs in controlling the aging process has been uncovered in different organisms. Recent studies have also shown that miRNAs regulate different components of proteostasis machinery as well as cellular response pathways to proteotoxic stress, some of which are very important during aging or in age-related pathologies. Here, we present a review of these findings, highlighting the role of individual miRNAs in age-associated protein folding and degradation across different organisms. We also broadly summarize the relationships between miRNAs and organelle-specific stress response pathways during aging and in various age-associated diseases.

衰老与受损和错误折叠蛋白质的积累有关,通过蛋白质稳态(蛋白质平衡)机制的下降,导致各种与年龄相关的蛋白质错误折叠疾病,如亨廷顿氏病或帕金森病。细胞应激反应途径的效率也随着年龄的增长而减弱,进一步导致维持蛋白质平衡的失败。MicroRNAs (miRNAs或miRs)是一类小的非编码rna (ncRNAs),它们在目标信使rna的3'UTR处结合,导致基因表达的转录后抑制。从在秀丽隐杆线虫中发现lin-4的衰老作用开始,许多mirna在控制衰老过程中的作用已经在不同的生物体中被发现。最近的研究还表明,mirna调节着蛋白质停滞机制的不同组成部分以及细胞对蛋白质毒性应激的反应途径,其中一些在衰老或年龄相关病理中非常重要。在这里,我们对这些发现进行了回顾,强调了个体mirna在不同生物体中与年龄相关的蛋白质折叠和降解中的作用。我们还概述了mirna与衰老和各种年龄相关疾病中细胞器特异性应激反应途径之间的关系。
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引用次数: 2
期刊
Non-Coding RNA
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