ABSTRACTPolyaromatic hydrocarbons (PAHs) released from vehicular emissions and oil refineries deposit on the phyllosphere, compromising the quality of leaf-based food products by posing many health issues. Nevertheless, the tea phyllosphere harbours a variety of endophytes that are highly effective at degrading polyaromatic hydrocarbons, anthracene, and pyrene. The present study attempts to analyse the pyrene and anthracene degrading capability of phyllosphere endophytic fungi that inhabit Camellia sinensis (L.) Kuntze leaves. The frequency of occurrence of endophytic fungi in different leaf tissue layers was examined using light and scanning electron microscopy (SEM). The best pyrene and anthracene degrading strains were selected based on the High-Performance Liquid Chromatography (HPLC) results, and further kinetic assays. Light microscopy and SEM observations highlighted a heterogeneous endophytic fungal distribution among leaf tissue layers; the upper epidermis had the highest fungal distribution compared to other leaf layers. HPLC results revealed that Phyllosticta capitalensis, Colletotrichum gloeosporioides, Colletotrichum siamense, Pseudopestalotiopsis chinensis, and Daldinia eschscholtzii, have higher pyrene and anthracene degradation respectively and their PAH degradation kinetics follow the first-order kinetic model. The best anthracene and pyrene degrader, P. capitalensis showed the lowest half-life. The present investigation highlights the potential of P. capitalensis, the best pyrene and anthracene degrader that can remediate PAHs deposited on the phyllosphere of tea leaves.KEYWORDS: Bioremediationfungal endophyteskineticspollutantspolyaromatic hydrocarbons (PAHs) AcknowledgmentsThe authors wish to acknowledge the Department of Plant and Molecular Biology, University of Kelaniya, Sri Lanka, for providing facilities to carry out this research component.Disclosure statementNo potential conflict of interest was reported by the author(s).
{"title":"Phylloremediation of pyrene and anthracene by endophytic fungi inhabiting tea leaves ( <i>Camellia sinensis</i> (L.) Kuntze) in Sri Lanka","authors":"Lanka Undugoda, Kasun Thambugala, Sagarika Kannangara, Jayantha Munasinghe, Nadeeka Premarathna, Nadeema Dharmasiri","doi":"10.1080/0028825x.2023.2258829","DOIUrl":"https://doi.org/10.1080/0028825x.2023.2258829","url":null,"abstract":"ABSTRACTPolyaromatic hydrocarbons (PAHs) released from vehicular emissions and oil refineries deposit on the phyllosphere, compromising the quality of leaf-based food products by posing many health issues. Nevertheless, the tea phyllosphere harbours a variety of endophytes that are highly effective at degrading polyaromatic hydrocarbons, anthracene, and pyrene. The present study attempts to analyse the pyrene and anthracene degrading capability of phyllosphere endophytic fungi that inhabit Camellia sinensis (L.) Kuntze leaves. The frequency of occurrence of endophytic fungi in different leaf tissue layers was examined using light and scanning electron microscopy (SEM). The best pyrene and anthracene degrading strains were selected based on the High-Performance Liquid Chromatography (HPLC) results, and further kinetic assays. Light microscopy and SEM observations highlighted a heterogeneous endophytic fungal distribution among leaf tissue layers; the upper epidermis had the highest fungal distribution compared to other leaf layers. HPLC results revealed that Phyllosticta capitalensis, Colletotrichum gloeosporioides, Colletotrichum siamense, Pseudopestalotiopsis chinensis, and Daldinia eschscholtzii, have higher pyrene and anthracene degradation respectively and their PAH degradation kinetics follow the first-order kinetic model. The best anthracene and pyrene degrader, P. capitalensis showed the lowest half-life. The present investigation highlights the potential of P. capitalensis, the best pyrene and anthracene degrader that can remediate PAHs deposited on the phyllosphere of tea leaves.KEYWORDS: Bioremediationfungal endophyteskineticspollutantspolyaromatic hydrocarbons (PAHs) AcknowledgmentsThe authors wish to acknowledge the Department of Plant and Molecular Biology, University of Kelaniya, Sri Lanka, for providing facilities to carry out this research component.Disclosure statementNo potential conflict of interest was reported by the author(s).","PeriodicalId":19317,"journal":{"name":"New Zealand Journal of Botany","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136315018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-12DOI: 10.1080/0028825x.2023.2256295
Tammo Reichgelt, Jennifer M. Bannister, William G. Lee
{"title":"Leaf hydathode occurrence in indigenous New Zealand angiosperm trees: a preliminary survey","authors":"Tammo Reichgelt, Jennifer M. Bannister, William G. Lee","doi":"10.1080/0028825x.2023.2256295","DOIUrl":"https://doi.org/10.1080/0028825x.2023.2256295","url":null,"abstract":"","PeriodicalId":19317,"journal":{"name":"New Zealand Journal of Botany","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135878049","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-07DOI: 10.1080/0028825x.2023.2251933
K.C. Burns
{"title":"How arboreal are epiphytes? A correction and brief commentary on Burns (2010)","authors":"K.C. Burns","doi":"10.1080/0028825x.2023.2251933","DOIUrl":"https://doi.org/10.1080/0028825x.2023.2251933","url":null,"abstract":"","PeriodicalId":19317,"journal":{"name":"New Zealand Journal of Botany","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2023-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41526245","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-05DOI: 10.1080/0028825x.2023.2245786
C. Cornwall, Wendy A. Nelson, J. D. Aguirre, Caitlin O. Blain, Lucy Coyle, R. D’archino, M. Desmond, Christopher D. Hepburn, L. Liggins, N. Shears, Mads S. Thomsen
{"title":"Predicting the impacts of climate change on New Zealand’s seaweed-based ecosystems","authors":"C. Cornwall, Wendy A. Nelson, J. D. Aguirre, Caitlin O. Blain, Lucy Coyle, R. D’archino, M. Desmond, Christopher D. Hepburn, L. Liggins, N. Shears, Mads S. Thomsen","doi":"10.1080/0028825x.2023.2245786","DOIUrl":"https://doi.org/10.1080/0028825x.2023.2245786","url":null,"abstract":"","PeriodicalId":19317,"journal":{"name":"New Zealand Journal of Botany","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2023-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47424642","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-08-24DOI: 10.1080/0028825x.2023.2240737
L. Perrie, Rémy Amice, L. Shepherd, P. Brownsey
{"title":"Tmesipteris alticola (Psilotaceae), a new fern species endemic to New Caledonia","authors":"L. Perrie, Rémy Amice, L. Shepherd, P. Brownsey","doi":"10.1080/0028825x.2023.2240737","DOIUrl":"https://doi.org/10.1080/0028825x.2023.2240737","url":null,"abstract":"","PeriodicalId":19317,"journal":{"name":"New Zealand Journal of Botany","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2023-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47419910","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-08-23DOI: 10.1080/0028825x.2023.2248933
Gautam Dutta, R. Singh
{"title":"Five new records of Annulohypoxylon spp. (Hypoxylaceae) from India","authors":"Gautam Dutta, R. Singh","doi":"10.1080/0028825x.2023.2248933","DOIUrl":"https://doi.org/10.1080/0028825x.2023.2248933","url":null,"abstract":"","PeriodicalId":19317,"journal":{"name":"New Zealand Journal of Botany","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2023-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45911814","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-08-20DOI: 10.1080/0028825x.2023.2245776
Hannah C. Rogers, B. Clarkson
{"title":"Epiphyte-host relationships of remnant and recombinant urban ecosystems in Hamilton, New Zealand: the importance of Dicksonia squarrosa (G.Forst.) Sw., whekī","authors":"Hannah C. Rogers, B. Clarkson","doi":"10.1080/0028825x.2023.2245776","DOIUrl":"https://doi.org/10.1080/0028825x.2023.2245776","url":null,"abstract":"","PeriodicalId":19317,"journal":{"name":"New Zealand Journal of Botany","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2023-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43544773","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-08-07DOI: 10.1080/0028825x.2023.2240752
C. Lusk, Mereana Atatoa Carr
{"title":"Canopy structure and understorey light availability in Nothofagus and podocarp-broadleaf stands in a New Zealand forest","authors":"C. Lusk, Mereana Atatoa Carr","doi":"10.1080/0028825x.2023.2240752","DOIUrl":"https://doi.org/10.1080/0028825x.2023.2240752","url":null,"abstract":"","PeriodicalId":19317,"journal":{"name":"New Zealand Journal of Botany","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2023-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45168934","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-07-12DOI: 10.1080/0028825x.2023.2232332
J. L. R. Lima, Juliana Rayssa Barros Felix, Hannah da Silva Vieira, C. Nobre, Juliana Aparecida Souza Leroy, P. O. Fiuza, B. Goto
{"title":"Recovering arbuscular mycorrhizal fungi (AMF) from decomposing litter: an unusual approach","authors":"J. L. R. Lima, Juliana Rayssa Barros Felix, Hannah da Silva Vieira, C. Nobre, Juliana Aparecida Souza Leroy, P. O. Fiuza, B. Goto","doi":"10.1080/0028825x.2023.2232332","DOIUrl":"https://doi.org/10.1080/0028825x.2023.2232332","url":null,"abstract":"","PeriodicalId":19317,"journal":{"name":"New Zealand Journal of Botany","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2023-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48063764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-29DOI: 10.1080/0028825x.2023.2228250
Jennifer Alderton-Moss, A. Munkacsi, K. van der Walt, C. Lehnebach
{"title":"Identification of fungal associates of the swamp helmet orchid (Corybas carsei), a threatened (nationally critical) species","authors":"Jennifer Alderton-Moss, A. Munkacsi, K. van der Walt, C. Lehnebach","doi":"10.1080/0028825x.2023.2228250","DOIUrl":"https://doi.org/10.1080/0028825x.2023.2228250","url":null,"abstract":"","PeriodicalId":19317,"journal":{"name":"New Zealand Journal of Botany","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2023-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42885856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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