Nitric oxide : biology and chemistry最新文献

Stable isotope measurement of in vivo nitric oxide production in health and disease: an updated systematic review and meta-analysis 健康和疾病中体内一氧化氮生成的稳定同位素测量：最新的系统综述和荟萃分析。

IF 3.2 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Nitric oxide : biology and chemistry

Pub Date : 2026-04-01 Epub Date: 2026-01-17 DOI: 10.1016/j.niox.2026.01.001

Thomas Loftus , Jonas Benjamim , Mauro Vaccarezza , Blossom CM. Stephan , Van Hoang Nguyen , Ammar Ashor , Mario Siervo

Background

Nitric oxide (NO) is involved in the regulation of vascular, immune and metabolic functions. Physiological modelling of stable isotope tracers provides an accurate method to measure whole-body NO production in humans. A systematic review and meta-analysis of studies using stable isotope methods was conducted to measure in vivo NO production in healthy individuals and patients with various disease conditions, characterise production rates across different populations and assess methodological factors contributing to measurement variability.

Methods

PubMed/MEDLINE, Embase, Scopus and Web of Science databases were searched from inception to April 2025. Random-effect models were used to estimate of NO production. Risk of bias was assessed using the BIOCROSS scale for biomarker studies. Publication bias was evaluated by Funnel Plots and Egger's regression test.

Results

58 studies were included in the systematic review, and 42 had valid data to be included in the meta-analysis. Mean NO production in healthy adults was 0.74 (95 %CI 0.47, 1.00) μmol·kg⁻¹·hour⁻¹ and ranged from 0.73 to 4.89 μmol kg⁻¹·hour⁻¹ depending on methodology and population characteristics. NO production in cardiovascular diseases [0.19 (95 %CI 0.11, 0.28) μmol·kg⁻¹·hour⁻¹] and metabolic diseases [0.43 (95 %CI 0.21, 0.64) μmol·kg⁻¹·hour⁻¹] was associated with lower physiological NO production. Conversely, chronic kidney disease [5.42 (95 %CI 2.04, 8.81) μmol·kg⁻¹·hour⁻¹] and inflammatory conditions [1.35 (95 %CI 0.78, 1.92) μmol·kg⁻¹·hour⁻¹] were associated with increased NO production.

Conclusions

Chronic metabolic and cardiovascular diseases were overall characterised by a lower NO production. Standardisation of stable isotope protocols and reference ranges are needed to improve clinical utility for monitoring and therapy.

背景：一氧化氮（NO）参与血管、免疫和代谢功能的调节。稳定同位素示踪剂的生理建模提供了一种精确的方法来测量人体全身NO的产生。对使用稳定同位素方法测量健康个体和各种疾病患者体内NO生成的研究进行了系统回顾和荟萃分析，表征了不同人群的产生率，并评估了导致测量变异性的方法因素。方法：检索PubMed/MEDLINE、Embase、Scopus和Web of Science数据库，检索时间为建库至2025年4月。随机效应模型用于估计NO的产生。使用生物标志物研究的bicross量表评估偏倚风险。采用漏斗图和Egger回归检验评价发表偏倚。结果：58项研究纳入系统评价，42项研究有有效数据纳入meta分析。健康成人的平均NO产生量为0.74 μmol·kg-1·h -1 (95%CI 0.47, 1.00)，根据方法和种群特征的不同，其范围为0.73 ~ 4.89 μmol·kg-1·h -1。心血管疾病[0.19 (95%CI 0.11, 0.28) μmol·kg-1·h -1]和代谢性疾病[0.43 (95%CI 0.21, 0.64) μmol·kg-1·h -1]的NO生成量较低。相反，慢性肾病[5.42 (95%CI 2.04, 8.81) μmol·kg-1·小时-1]和炎症[1.35 (95%CI 0.78, 1.92) μmol·kg-1·小时-1]与一氧化氮生成增加相关。结论：慢性代谢和心血管疾病的总体特征是一氧化氮的产生较低。为了提高监测和治疗的临床效用，稳定同位素方案和参考范围的标准化是必要的。

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引用次数: 0

Architectural modulation of polymeric hydrogen sulfide donors: Structure–responsive materials for controlled therapeutics 聚合硫化氢供体的结构调节：用于控制治疗的结构响应材料。

IF 3.2 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Nitric oxide : biology and chemistry

Pub Date : 2026-04-01 Epub Date: 2026-02-09 DOI: 10.1016/j.niox.2026.02.001

Vivek Pandey , Nikky Sharma , Tejasvi Pandey

Hydrogen sulfide (H₂S), once regarded merely as a malodorous and toxic gas, has emerged as a vital endogenous signaling molecule with multifaceted physiological roles, encompassing neuromodulation, vasorelaxation, cytoprotection, and the regulation of inflammatory responses. Despite this growing recognition of its biological significance, the therapeutic translation of H₂S remains impeded by its physicochemical limitations as volatility, poor aqueous solubility, and rapid metabolic clearance. Addressing these challenges has stimulated the development of polymeric hydrogen sulfide (H₂S) donors engineered macromolecular systems capable of releasing H₂S in a temporally and spatially controlled manner. These structure-responsive materials offer a powerful platform wherein molecular architecture, donor chemistry, and supramolecular organization can be finely tuned to regulate release kinetics and responsiveness to physiological stimuli such as pH variations, redox gradients, and reactive oxygen species (ROS). This review consolidates recent advances in the design and application of polymeric and macromolecular H₂S donors, emphasizing the mechanistic relationships between structural parameters polymer topology, donor incorporation strategy, linker chemistry, hydrophilicity, and crosslinking density and their resultant release behaviors. Furthermore, it highlights the expanding biomedical landscape of these materials, discussing their therapeutic potential, translational challenges, and emerging strategies for precision engineering. By bridging molecular-level design with macroscopic functionality, this review aims to inform the rational development of next-generation polymeric H₂S donors that enable finely controlled spatiotemporal delivery and enhanced therapeutic efficacy.

硫化氢（H2S）曾经仅仅被认为是一种恶臭和有毒气体，现在已经成为一种重要的内源性信号分子，具有多方面的生理作用，包括神经调节、血管松弛、细胞保护和炎症反应调节。尽管人们越来越认识到H2S的生物学意义，但其挥发性、水溶性差、代谢清除快等理化限制仍阻碍了H2S的治疗翻译。为了解决这些问题，人们开发了聚合物硫化氢（H2S）供体，设计了能够以时间和空间可控的方式释放H2S的大分子系统。这些结构响应材料提供了一个强大的平台，其中分子结构、供体化学和超分子组织可以精细调节释放动力学和对生理刺激的响应，如pH变化、氧化还原梯度和活性氧（ROS）。本文综述了聚合物和大分子H2S给体的设计和应用方面的最新进展，重点介绍了结构参数、聚合物拓扑结构、给体掺入策略、连接剂化学、亲水性、交联密度及其释放行为之间的机理关系。此外，它还强调了这些材料不断扩大的生物医学领域，讨论了它们的治疗潜力、转化挑战和精密工程的新兴策略。通过将分子水平设计与宏观功能相结合，本综述旨在为下一代聚合物H2S供体的合理开发提供信息，使其能够精细控制时空传递并提高治疗效果。

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引用次数: 0

Analytic performance characteristics of the chemical-reduction gas phase-chemiluminescence assay for the quantification of nitric oxide metabolites 化学还原气相化学发光法测定一氧化氮代谢物的分析性能特点。

IF 3.2 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Nitric oxide : biology and chemistry

Pub Date : 2026-04-01 Epub Date: 2025-12-29 DOI: 10.1016/j.niox.2025.12.002

Michelle Menon Miyake , Ipsita Mohanty , Sylvia N. Michki , Edwin Tamashiro , Noam A. Cohen , Harry Ischiropoulos

Nitric oxide metabolites (NOm) in biological fluids have been widely used as surrogate markers to inform changes in nitric oxide-mediated signaling, which is critical for many biological functions. One method for quantifying NOm employs chemical reduction to convert the various metabolites into nitric oxide, followed by ozone-based chemiluminescence detection. Herein, we tested and quantified the bioanalytical parameters of the reduction-chemiluminescence assay and propose conditions for optimizing the assay. The slopes of the standard curves used for quantifying the metabolites showed a 3.8 % intra-assay and 7.6 % inter-assay relative standard deviation (%RSD). An 8 % and 8.5 % RSD was measured for two separate quality control serum samples, and a Levey-Jennings plot showed that all individual values fell within 1 standard deviation of the mean of all measurements. Repeat analysis of human serum samples (n = 51) resulted in an average incurred sample reanalysis of 7.7 %, with 49 samples having repeated results above the original value, indicating no loss of NOm over the four-week period. This was further confirmed by recovery experiments, which indicated average recovery rates of 100.4 ± 8.2 % and 99.3 ± 4.3 % for two operators, respectively. ANOVA of data from two operators indicated that sample-to-sample variability was the main contributor to the total variance. Overall, our results demonstrate that the assay provides appropriate and reproducible quantification of NOm, supporting its use for both exploratory and routine laboratory applications.

生物体液中的一氧化氮代谢物（NOm）已被广泛用作替代标记物，以告知一氧化氮介导的信号传导的变化，这对许多生物功能至关重要。一种量化NOm的方法采用化学还原将各种代谢物转化为一氧化氮，然后进行基于臭氧的化学发光检测。在此，我们测试和量化了还原化学发光法的生物分析参数，并提出了优化分析的条件。测定代谢物的标准曲线斜率为3.8%，测定间相对标准偏差（%RSD）为7.6%。两份单独的质控血清样本的RSD分别为8%和8.5%，Levey-Jennings图显示所有个体值均在所有测量值均值的1个标准差范围内。对人血清样本（n=51）的重复分析导致平均7.7%的样本重复分析，其中49个样本的重复结果高于原始值，表明在四周期间没有NOm损失。回收率实验进一步证实了这一点，两种操作方法的平均回收率分别为100.4±8.2%和99.3±4.3%。方差分析从两个运营商的数据表明，样本到样本的可变性是总方差的主要贡献者。总的来说，我们的结果表明，该方法提供了适当的、可重复的NOm定量，支持其用于探索性和常规实验室应用。

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引用次数: 0

Therapeutic potential of S-nitroso-l-cysteine in improving cardiorenal function in eNOS-KO CKD mice s -亚硝基半胱氨酸改善eNOS-KO CKD小鼠心肾功能的治疗潜力。

IF 3.2 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Nitric oxide : biology and chemistry

Pub Date : 2026-04-01 Epub Date: 2025-12-28 DOI: 10.1016/j.niox.2025.12.003

Xiaofan Wu , Jiahe Zhao , Zhengqi Xu , Feng Jiang , Moran Wang , Jingwen Tao , Cuntai Zhang , Li Lin , Sheng Li

Objective

Patients with chronic kidney disease (CKD) usually exhibit high incidence and mortality rates of cardiovascular disease (CVD). Patients with CKD typically exhibit endothelial dysfunction, which represents one of the risk factors for CVD. As a signaling molecule, S-nitroso-l-cysteine (CSNO) plays a pivotal role in endothelial function. In this study, we explored the therapeutic value of CSNO on eNOS-KO CKD mice.

Methods

Male eNOS-KO mice were randomly assigned to one of three groups: control, adenine-induced CKD, and CKD with CSNO nebulization (88 ppm, 20 min/day for 6 weeks). Cardiac function was assessed via Doppler echocardiography. Fibrosis, hypertrophy, lipid accumulation, ROS production, and apoptosis were evaluated by HE, Masson, WGA, Oil Red O, DHE, and TUNEL staining, respectively.

Results

CSNO treatment significantly increased the kidney-to-body weight ratio compared to the eNOS-KO CKD group, and urinary creatinine levels exhibited an increasing trend, although this change was not statistically significant. Furthermore, CSNO markedly attenuated renal lipid accumulation. Echocardiography revealed that CSNO significantly enhanced left ventricular ejection fraction (LVEF) and tissue Doppler E'/A′ ratio. The E/A ratio also increased in the CSNO treatment group, but this change was not statistically significant. Moreover, CSNO alleviated cardiac hypertrophy, decreased ROS production, apoptosis, and lipid accumulation compared to the eNOS-KO CKD group.

Conclusion

Collectively, the findings of the present study demonstrated that CSNO improved cardiorenal function in eNOS-KO CKD mice, thereby affording a novel therapeutic approach to treat CKD patients with endothelial dysfunction.

目的：慢性肾脏疾病（CKD）患者通常表现为心血管疾病（CVD）的高发病率和死亡率。CKD患者通常表现为内皮功能障碍，这是心血管疾病的危险因素之一。作为一种信号分子，s -亚硝基半胱氨酸在内皮功能中起着至关重要的作用。在本研究中，我们探讨了CSNO对eNOS-KO CKD小鼠的治疗价值。方法：将雄性eNOS-KO小鼠随机分为三组：对照组、腺嘌呤诱导的CKD组和CSNO雾化组（88 ppm， 20分钟/天，持续6周）。通过多普勒超声心动图评估心功能。分别通过HE、Masson、WGA、Oil Red O、DHE和TUNEL染色评估纤维化、肥大、脂质积累、ROS产生和凋亡。结果：与eNOS-KO CKD组相比，CSNO治疗显著增加了肾体重比，尿肌酐水平呈上升趋势，但这种变化无统计学意义。此外，CSNO可显著减轻肾脏脂质积聚。超声心动图显示，CSNO显著提高左室射血分数（LVEF）和组织多普勒E‘/A’比值。CSNO治疗组E/A比值升高，但无统计学意义。此外，与eNOS-KO CKD组相比，CSNO减轻了心脏肥厚，减少了ROS的产生，细胞凋亡和脂质积累。结论：总的来说，本研究的结果表明，CSNO改善eNOS-KO CKD小鼠的心肾功能，从而为治疗伴有内皮功能障碍的CKD患者提供了一种新的治疗方法。

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引用次数: 0

Prognostic and monitoring relevance of systemic nitric oxide in patients with colon cancer. 结肠癌患者全身一氧化氮的预后和监测相关性。

IF 3.2 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Nitric oxide : biology and chemistry

Pub Date : 2026-03-13 DOI: 10.1016/j.niox.2026.03.001

Rania-Sihem Boussa, Manel Amri, Imene Soufli, Houda Belguendouz, Lila Khelifi, Fahima Ameur, Meriem-Fella Guerni, Lilya Khelifi, Hakim Chatter, Batoul Latreche, Assia Slimani, Nassim Sid-Idris, Chafia Touil-Boukoffa

Reliable and minimally invasive biomarkers are needed for early detection, prognosis, and monitoring of colon cancer (CC). The aim of our study was to evaluate the role of systemic nitric oxide (NO) in inflammation and tumor progression and its diagnostic and prognostic value in CC. This prospective monocentric case-control study included 130 patients with non-metastatic CC and 100 healthy subjects. Clinical and pathological data were collected, and blood cell count-derived ratios were calculated. Systemic nitrite and cytokines levels were assessed using Griess method and ELISA respectively. Tumor-infiltrating immune cells were evaluated by immunohistochemistry in 44 patients. ROC curves, Kaplan-Meier, and log-rank tests were performed to highlight the diagnostic and prognostic value of NO production in patients followed for two years. Systemic plasma nitrite levels were significantly higher in patients than in controls (p < 0.0001), and correlated significantly and positively with most inflammatory and tumor markers and CD68⁺ cells and CD4⁺ T cells, but negatively with IL-6, albumin and CD8⁺ T cells. Interestingly, high nitrite levels were associated with tumor progression (cancer stage, tumor size, lymph node ratio). ROC analysis confirmed a high diagnostic performance (AUC = 0.88 for patients vs. controls; AUC = 0.71 for early vs. advanced stages). Prognostically, elevated nitrite levels predicted postoperative complications (AUC = 0.67; p log-rank<0.0001), reduced recurrence-free survival (AUC = 0.70; p log-rank = 0.03), and reduced overall survival (AUC = 0.75; p log-rank = 0.04). Our findings indicate that systemic nitrite levels are closely linked to inflammation and tumor progression in CC and demonstrate strong predictive accuracy, emerging as a promising biomarker for patient management.

可靠和微创的生物标志物是早期发现、预后和监测结肠癌（CC）的必要条件。本研究的目的是评估全身一氧化氮（NO）在CC炎症和肿瘤进展中的作用及其诊断和预后价值，这项前瞻性单中心病例对照研究包括130名非转移性CC患者和100名健康受试者。收集临床和病理资料，计算血细胞计数衍生比。采用Griess法和ELISA法分别测定全身亚硝酸盐和细胞因子水平。采用免疫组化方法对44例患者的肿瘤浸润性免疫细胞进行检测。采用ROC曲线、Kaplan-Meier和log-rank检验来强调随访两年患者NO生成的诊断和预后价值。患者全身血浆亚硝酸盐水平明显高于对照组(p

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引用次数: 0

Compartment-specific nitric oxide signaling in mouse cardiac endothelial cells: Intra- and extracellular measurements and the impact of cryopreservation. 小鼠心脏内皮细胞室特异性一氧化氮信号：细胞内和细胞外测量和低温保存的影响。

IF 3.2 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Nitric oxide : biology and chemistry

Pub Date : 2026-02-27 DOI: 10.1016/j.niox.2026.02.004

Elham Ashrafi, Janet A W Elliott

Nitric oxide (NO) produced by endothelial cells plays a central role in regulating vascular tone, blood flow, and inflammatory responses. Although NO freely diffuses across cell membranes, intracellular and extracellular NO-related signals are often measured interchangeably, complicating interpretation of NO production, signaling, and bioavailability. Moreover, how cryopreservation and experimental context influence these two measurement compartments remains poorly defined. Here, we systematically compared intracellular and extracellular NO-related fluorescence in mouse cardiac endothelial cells (MCECs) under defined stimulatory, inhibitory, and culture conditions. Using DAF-FM DA (4-amino-5-methylamino-2',7'-difluorofluorescein diacetate) and DAF-FM-based assays, we examined (i) the impact of cryopreservation on intra- and extracellular NO-related signals following calcium ionophore A23187 stimulation, and (ii) the effects of physiological stimulation (bradykinin), enzymatic inhibition (Nᴳ-Nitro-L-arginine methyl ester (L-NAME)), and NO scavenging (carboxy-PTIO potassium salt (cPTIO)) in non-cryopreserved cells. Calcium ionophore A23187 increased intracellular NO-related fluorescence across non-cryopreserved and post-thaw groups, whereas extracellular responses were smaller and more variable, with increases observed primarily in glycerol-cryopreserved cells. The removal of phenol red from the medium enhanced extracellular NO-related fluorescence (reflecting improved detection sensitivity) but reduced intracellular signals, indicating a strong influence of medium composition on NO-related measurements. Bradykinin produced dose-dependent increases in extracellular signals accompanied by reductions in intracellular signals, consistent with rapid diffusion and context-dependent intracellular detection. L-NAME reduced intracellular fluorescence and suppressed extracellular signals only under simplified buffer conditions, while cPTIO produced modest reductions in extracellular fluorescence with substantial variability. Together, these findings indicate that intracellular and extracellular NO-related fluorescence are differentially regulated by stimulation, membrane properties, oxidative environment, and assay conditions. The results underscore the importance of compartment-specific, method-aware interpretation when using fluorescence-based approaches to assess NO signaling in endothelial cell models, particularly in the context of cryopreservation and experimental reproducibility.

一氧化氮（NO）由内皮细胞产生，在调节血管张力、血流和炎症反应中起核心作用。虽然一氧化氮可以自由地在细胞膜上扩散，但细胞内和细胞外的一氧化氮相关信号通常是交替测量的，这使一氧化氮的产生、信号传导和生物利用度的解释变得复杂。此外，低温保存和实验环境如何影响这两个测量隔间仍然不清楚。在这里，我们系统地比较了小鼠心脏内皮细胞（MCECs）在特定的刺激、抑制和培养条件下的细胞内和细胞外no相关荧光。利用DAF-FM DA（4-氨基-5-甲氨基-2′，7′-二氟荧光素双乙酸酯）和基于DAF-FM的检测，我们研究了(i)低温保存对钙离子载体A23187刺激后细胞内外NO相关信号的影响，以及（ii）生理刺激（缓激肽）、酶抑制（N - o-硝基- l -精氨酸甲酯（L-NAME））和NO清除（羧基- ptio钾盐（cPTIO））在非低温保存细胞中的作用。钙离子载体A23187在非冷冻保存组和解冻后组中增加了细胞内no相关荧光，而细胞外反应较小且变化更大，主要在甘油冷冻保存的细胞中观察到增加。从培养基中去除酚红增强了细胞外no相关荧光（反映了检测灵敏度的提高），但降低了细胞内信号，表明培养基成分对no相关测量有很强的影响。缓激肽产生剂量依赖性细胞外信号增加，同时细胞内信号减少，与快速扩散和环境依赖性细胞内检测一致。L-NAME仅在简化的缓冲条件下降低细胞内荧光并抑制细胞外信号，而cPTIO对细胞外荧光产生适度的降低，且具有很大的可变性。总之，这些发现表明，细胞内和细胞外no相关的荧光受到刺激、膜性质、氧化环境和测定条件的不同调节。研究结果强调了当使用基于荧光的方法评估内皮细胞模型中的NO信号时，特别是在低温保存和实验可重复性的背景下，室特异性、方法感知解释的重要性。

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引用次数: 0

Stabilizing nitric oxide: MnFe2O4@Cap-SNO nanoparticles and chitosan hydrogels for controlled therapeutic delivery 稳定一氧化氮：MnFe2O4@Cap-SNO纳米颗粒和壳聚糖水凝胶用于控制治疗递送。

IF 3.2 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Nitric oxide : biology and chemistry

Pub Date : 2026-02-01 Epub Date: 2025-11-06 DOI: 10.1016/j.niox.2025.10.008

Soodabeh Gharibeh , Melika JaberebnAnsari , Elham Askarizadeh , Azhdar Heydari

Nitric oxide (NO) is a vital bioactive molecule, but its rapid degradation limits therapeutic applications. To enhance stability and controlled release, two novel systems were synthesized: MnFe₂O₄@Cap-SNO nanoparticles and Cs@Cap-SNO hydrogel. MnFe₂O₄@Cap-SNO, incorporating captopril (Cap) as an NO donor, demonstrated prolonged NO release for up to 16h upon light exposure, following zero-order kinetics, ensuring sustained delivery. Cs@Cap-SNO, a chitosan-based hydrogel integrating nitrosated captopril (Cap-SNO), exhibited faster NO release governed by Korsmeyer-Peppas kinetics. UV–Vis spectrophotometry confirmed successful nitrosation with distinct absorption bands at 335nm and 545nm. MnFe₂O₄@Cap-SNO displayed superior stability, outperforming Cs@Cap-SNO and free Cap-SNO in NO preservation. These findings suggest MnFe₂O₄@Cap-SNO as an effective NO-stabilizing carrier for biomedical applications, particularly intargeted drug delivery. Future studies will focus on in vitro and in vivo validation to assess therapeutic efficacy.

一氧化氮（NO）是一种重要的生物活性分子，但其快速降解限制了其治疗应用。为了提高稳定性和控释，合成了两种新型体系：MnFe2O4@Cap-SNO纳米颗粒和Cs@Cap-SNO水凝胶。MnFe2O4@Cap-SNO，将卡托普利（Cap）作为NO供体，在光照下延长NO释放长达16小时，遵循零级动力学，确保持续递送。Cs@Cap-SNO是一种整合亚硝化卡托普（Cap-SNO）的壳聚糖水凝胶，在Korsmeyer-Peppas动力学的控制下，表现出更快的NO释放。紫外可见分光光度法证实亚硝化成功，在335 nm和545 nm处有不同的吸收带。MnFe2O4@Cap-SNO在NO保存方面表现出较好的稳定性，优于Cs@Cap-SNO和free Cap-SNO。这些发现表明MnFe2O4@Cap-SNO是生物医学应用中有效的no稳定载体，特别是靶向药物递送。未来的研究将集中在体外和体内验证，以评估治疗效果。

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引用次数: 0

Hydrogen sulfide and the physiology of “totonou”: a hypothesis on sauna-induced vascular recovery 硫化氢与“托托努”的生理：桑拿诱导血管恢复的假说。

IF 3.2 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Nitric oxide : biology and chemistry

Pub Date : 2026-02-01 Epub Date: 2025-11-19 DOI: 10.1016/j.niox.2025.11.004

Ryosuke Shinkai , Takashi Tomita

Sauna bathing, a traditional health practice in Finland and Japan, has been associated with reduced cardiovascular and neurological risks. Circulatory recovery, marked by a shift from sympathetic to parasympathetic dominance, underlies the relaxation state known as “totonou.” Traditionally attributed to nitric oxide (NO), this effect may also involve hydrogen sulfide (H₂S), a gaseous mediator with vasodilatory, antioxidant, and anti-inflammatory properties. We hypothesize that H₂S facilitates sauna-induced circulatory recovery and that environmental exposure may modulate this process. This perspective highlights H₂S as a novel regulator and calls for basic, clinical, and epidemiological studies to test this hypothesis.

桑拿洗浴是芬兰和日本的一种传统保健做法，与降低心血管和神经系统风险有关。以交感神经向副交感神经主导转变为标志的循环恢复，是被称为“放松状态”的放松状态的基础。传统上认为是一氧化氮（NO）的作用，这种作用也可能与硫化氢（H2S）有关，硫化氢是一种具有血管舒张、抗氧化和抗炎特性的气体介质。我们假设H2S促进桑拿诱导的循环恢复，而环境暴露可能调节这一过程。这一观点强调H2S是一种新型调节剂，需要进行基础、临床和流行病学研究来验证这一假设。

引用次数: 0

Prone positioning and inhaled nitric oxide in patients with acute respiratory distress syndrome: synergism or independent effects? 俯卧位与急性呼吸窘迫综合征患者吸入一氧化氮：协同作用还是独立作用？

IF 3.2 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Nitric oxide : biology and chemistry

Pub Date : 2026-02-01 Epub Date: 2025-11-28 DOI: 10.1016/j.niox.2025.11.008

Stefano Spina , Timothy G. Gaulton

引用次数: 0

Corrigendum to “Detection and proteomic identification of in vivo S-nitrosylated proteins in Vibrio cholerae: A novel evidence” [Nitric Oxide 159 (2025) 63–77] “霍乱弧菌体内s -亚硝基化蛋白的检测和蛋白质组学鉴定：一个新的证据”[一氧化氮159(2025)63-77]的勘误表。

IF 3.2 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Nitric oxide : biology and chemistry

Pub Date : 2026-02-01 Epub Date: 2025-11-18 DOI: 10.1016/j.niox.2025.11.003

Shuddhasattwa Samaddar , Surupa Chakraborty , Rajib Sengupta , Sanjay Ghosh

引用次数: 0