Nitric oxide : biology and chemistry最新文献_第5页

Biomechanical study on the effect of changes in ostiomeatal complex structure on NO concentration in the nasal cavity 口鼻道复合体结构变化对鼻腔NO浓度影响的生物力学研究

IF 3.2 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Nitric oxide : biology and chemistry

Pub Date : 2025-06-04 DOI: 10.1016/j.niox.2025.06.001

Shen Yu , Xiaojun Xie , Danqing Wang , Yan Guo , Jizhe Wang

Background

Nitric oxide (NO) concentration in the nasal cavity plays an important role in maintaining nasal function and health. We investigated the influence of the geometric structure of the ostiomeatal complex (OMC) on nasal cavity NO concentration distribution and provide optimization strategies for reconstruction surgery related to OMC function.

Method

ology: Numerical nasal cavity models of 21 healthy volunteers were established, and the effects of the OMC structure on NO distribution in the nasal cavity were numerically studied. Additionally, the nasal meatus widths of five healthy adult New Zealand rabbits were modified surgically. Exhaled NO concentration was measured pre- and post-surgery to study the effect of nasal meatus width on NO distribution in the rabbit nasal cavities.

Results

Through numerical analysis, a quantified relationship was obtained between the width of the middle nasal passage, the hydraulic diameter of the maxillary sinus ostium, and airflow rate with the concentration of NO in the nasal and paranasal sinuses. The impact of these three factors on the NO concentration of exhaled airflow was compared across all volunteers. An optimal combination of middle nasal passage width and ostium diameter can ensure a normal NO concentration in the nasal cavity.

Conclusions

When reconstructing the OMC structure to restore function, factors such as the width of the middle nasal meatus and maxillary sinus ostium area and length should be considered comprehensively to reshape an optimal OMC structure for achieving a normal physiological distribution of NO concentration in the nasal cavity.

鼻腔内一氧化氮（NO）浓度在维持鼻腔功能和健康中起着重要作用。我们研究了口鼻道复合体（OMC）的几何结构对鼻腔NO浓度分布的影响，并为OMC功能相关的重建手术提供优化策略。方法：建立21例健康志愿者鼻腔数值模型，数值研究OMC结构对鼻腔NO分布的影响。另外，对5只健康成年新西兰兔的鼻道宽度进行了手术修饰。术前、术后测定兔呼出一氧化氮浓度，研究鼻道宽度对兔鼻腔内一氧化氮分布的影响。结果通过数值分析，得到了鼻中通道宽度、上颌窦口水力直径、气流速率与鼻、鼻窦NO浓度之间的定量关系。比较了这三个因素对所有志愿者呼出气流中NO浓度的影响。鼻中通道宽度和鼻孔直径的最佳组合可以保证鼻腔内正常的NO浓度。结论在重建OMC结构以恢复功能时，应综合考虑鼻中道宽度和上颌窦口面积、长度等因素，重塑最佳的OMC结构，使鼻腔内NO浓度达到正常的生理分布。

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引用次数: 0

Antagonistic effect of a nitric oxide donor agents based on ruthenium complex combined with cisplatin on lung tumor cell lines 钌络合物联合顺铂的一氧化氮供体对肺肿瘤细胞系的拮抗作用。

IF 3.2 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Nitric oxide : biology and chemistry

Pub Date : 2025-05-31 DOI: 10.1016/j.niox.2025.05.009

Angelica E. Graminha , Amanda B. Becceneri , Rafaella R Rios , Márcia Regina Cominetti , Juliana Cristina Biazzotto , Roberto Santana da Silva

Nitric oxide (NO) is a versatile biological messenger involved in numerous physiological processes and anticancer mechanisms. Its functions are highly dependent on its concentration and the specific site of action. In this study, we investigated the effects of controlled NO release mediated by ruthenium-based compounds. The tests demonstrated the significant potential of combining cisplatin with the non-cytotoxic ruthenium nitrosyl complexes cis-[Ru(bpy)₂(NO₂)(solv)]PF₆ and cis-Ru(bpy)₂(NO)(pic)](PF₆)₃, where bpy = 2,2′-bipyridine ,pic = 4-picoline and solv = solvent. This combination increased selectivity between non-tumoral and tumoral lung cells (MRC-5/A549) compared to the selectivity index of cisplatin alone. These nitrosyl complexes exhibited an antagonistic interaction with cisplatin, reducing its cytotoxic efficacy. Cell cycle and apoptosis assays revealed that the cisplatin/Ru combination more effectively inhibited cisplatin's cytotoxic effect on the MRC-5 non-tumoral lung cell line compared to the A549 tumoral cell line. Morphological assays conducted in 3D culture with the cis-[Ru(bpy)₂(NO)(pic)](PF₆)₃ complex confirmed its chemopreventive behavior, as the 3D system closely mimics in vivo conditions. Moreover, the absence of cytotoxicity in these ruthenium nitrosyl complexes highlights their potential as promising candidates for adjuvant therapy in combination with other drugs.

一氧化氮（NO）是一种多用途的生物信使，参与许多生理过程和抗癌机制。它的功能高度依赖于它的浓度和特定的作用部位。在这项研究中，我们研究了钌基化合物介导的控制NO释放的影响。试验表明，顺铂与无细胞毒性的钌亚硝基配合物顺式-[Ru(bpy)2(NO2)(solv)]PF6和顺式-Ru(bpy)2(NO)(pic)](PF6)3结合具有显著的潜力，其中bpy = 2,2'-联吡啶，pic = 4-吡啶（顺铂/Ru）。与单用顺铂的选择性指数相比，联合用药增加了非肿瘤和肿瘤肺细胞（MRC-5/A549）之间的选择性。这些亚硝基复合物表现出与顺铂的拮抗相互作用，降低其细胞毒性作用。细胞周期和凋亡实验显示，与A549肿瘤细胞系相比，顺铂/Ru联合治疗更有效地抑制顺铂对MRC-5非肿瘤肺细胞系的细胞毒作用。用顺式-[Ru(bpy)2(NO)(pic)](PF6)3配合物在三维培养中进行的形态学分析证实了其化学预防作用，因为三维系统非常接近体内条件。此外，这些钌亚硝基络合物没有细胞毒性，突出了它们作为与其他药物联合辅助治疗的有希望的候选者的潜力。

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引用次数: 0

Targeting inducible nitric oxide synthase with 1400W mitigates septic acute lung injury through inhibiting SLC7A11/GPX4 mediated ferroptosis 1400W靶向诱导型一氧化氮合酶，通过抑制SLC7A11/GPX4介导的铁凋亡减轻脓毒性急性肺损伤

IF 3.2 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Nitric oxide : biology and chemistry

Pub Date : 2025-05-28 DOI: 10.1016/j.niox.2025.05.008

Zi-yao Wang , Tao Zeng , Xin Wang , Xin Zhuo , Jing-wen Zheng , Ling Zhu , Shu-ting Cheng , Li-hong Wan

Accumulating evidence has indicated that lung ferroptosis is an important contributor to septic acute lung injury (SALI). Inducible nitric oxide synthase (iNOS) may be implicated in the regulation of bronchial epithelial ferroptosis. Nevertheless, the precise mechanisms by which iNOS modulates ferroptosis remain elusive. This study investigated whether iNOS selective inhibitor 1400w alleviates LPS-induced SALI and suppresses ferroptosis in mice. Additionally, RNA sequencing (RNA-seq), molecular docking, molecular dynamic simulation, Transmission electron microscope (TEM), and western blotting were employed to predict and evaluate the molecular mechanism of 1400w on LPS-induced ferroptosis in vivo. The results showed that the administration of 1400w markedly attenuated LPS-induced lung injury and facilitated pulmonary function in mice. Also, 1400w administration effectively suppressed bronchial epithelial ferroptosis induced by LPS in mice. Furthermore, molecular docking and molecular dynamics simulations revealed stable binding between GPX4 and iNOS, with 1400w modulating ferroptosis mediated by SLC7A11/GPX4 through targeting iNOS. Collectively, our research demonstrated that inhibition of iNOS might represent a potential therapeutic strategy to improve SALI by inhibiting ferroptosis.

越来越多的证据表明，肺铁下垂是脓毒性急性肺损伤（SALI）的重要因素。诱导型一氧化氮合酶（iNOS）可能参与支气管上皮铁下垂的调节。然而，iNOS调节铁下垂的确切机制仍然难以捉摸。本研究探讨iNOS选择性抑制剂1400w是否能减轻lps诱导的小鼠SALI并抑制铁下垂。采用RNA测序（RNA-seq）、分子对接、分子动力学模拟、透射电镜（TEM）、western blotting等方法预测和评价1400w对lps诱导铁下垂的分子机制。结果表明，1400w可明显减轻lps诱导的小鼠肺损伤，促进肺功能。1400w给药可有效抑制LPS诱导的小鼠支气管上皮铁下垂。此外，分子对接和分子动力学模拟揭示了GPX4与iNOS之间的稳定结合，SLC7A11/GPX4通过靶向iNOS介导1400w调制铁死亡。总的来说，我们的研究表明，抑制iNOS可能是一种通过抑制铁下垂来改善SALI的潜在治疗策略。

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引用次数: 0

Comprehensive evaluation of S-nitrosoglutathione and S-nitroso-N-acetylpenicillamine stability in biomedical contexts s -亚硝基谷胱甘肽和s -亚硝基-n -乙酰青霉胺在生物医学领域稳定性的综合评价。

IF 3.2 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Nitric oxide : biology and chemistry

Pub Date : 2025-05-27 DOI: 10.1016/j.niox.2025.05.006

Qingqing Fan , Shu Geng , Olivia Rusli , Federico Mazur , Zifei Han , Nicole Joy Rijs , Rona Chandrawati

Nitric oxide (NO) is a potent signaling molecule with great therapeutic potential. However, the clinical application of direct NO delivery is limited by its short half-life and high reactivity, which creates challenges for effective controlled delivery. To overcome these limitations, S-nitrosothiols (RSNOs) are commonly used as NO donors in therapeutic applications, as they stabilize the short-lived free radical and improve NO pharmacokinetics. Despite their widespread use, the stability and release kinetics of RSNOs under physiological conditions have not been thoroughly evaluated, which is crucial for determining their therapeutic efficacy and safety. This study provides a comprehensive evaluation of the stability and in vitro NO release profiles of two commonly used RSNOs, S-nitrosoglutathione (GSNO) and S-nitroso-N-acetylpenicillamine (SNAP), at physiologically relevant conditions. Using a range of analytical techniques, including electrospray ionization mass spectrometry, Griess assay, and electrochemical sensing, we assessed RSNO stability across various conditions, including different buffers, pH levels, temperatures, as well as exposure to UV light irradiation to simulate common sterilization practices. Additionally, we investigated RSNO stability in cell culture media with varying glucose levels and serum compositions to better mimic biological environments. Our findings provide critical insights into the factors affecting RSNO stability and NO release, advancing the development of more effective NO-based therapies and biomedical devices.

一氧化氮（NO）是一种有效的信号分子，具有很大的治疗潜力。然而，直接给药一氧化氮的临床应用受到半衰期短和高反应性的限制，这给有效的控制给药带来了挑战。为了克服这些限制，s -亚硝基硫醇（RSNOs）通常被用作治疗应用中的NO供体，因为它们稳定了短寿命的自由基并改善了NO的药代动力学。尽管RSNOs被广泛使用，但其在生理条件下的稳定性和释放动力学尚未得到全面评估，这对确定其治疗效果和安全性至关重要。本研究在生理相关条件下，对两种常用的RSNOs s -亚硝基谷胱甘肽（GSNO）和s -亚硝基-n -乙酰青霉胺（SNAP）的稳定性和体外NO释放谱进行了综合评价。使用一系列分析技术，包括电喷雾电离质谱法、Griess测定法和电化学传感，我们评估了RSNO在不同条件下的稳定性，包括不同的缓冲液、pH值、温度，以及暴露在紫外线照射下，以模拟常见的灭菌操作。此外，我们研究了RSNO在不同葡萄糖水平和血清成分的细胞培养基中的稳定性，以更好地模拟生物环境。我们的研究结果对影响一氧化氮稳定性和一氧化氮释放的因素提供了重要的见解，推动了更有效的一氧化氮治疗和生物医学设备的发展。

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引用次数: 0

Effect of acute nitrate supplementation on the superficial conduit venous vascular response at rest and during sympathoexcitation 急性硝酸补充对静息和交感神经兴奋时浅管静脉血管反应的影响。

IF 3.2 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Nitric oxide : biology and chemistry

Pub Date : 2025-05-27 DOI: 10.1016/j.niox.2025.05.007

Anna Oue, Momo Ito, Yasuhiro Iimura, Naho Serizawa, Yuichi Miyakoshi, Masako Ota

The effects of increased nitric oxide (NO) activity and/or NO precursor levels following nitrate (NO₃⁻) supplementation on venous vascular control remain poorly understood. We investigated the effect of acute NO₃⁻ supplementation on the venous vascular response of a single conduit vein at rest and during sympathoexcitation (e.g., static exercise and muscle metaboreflex). Participants were 15 healthy young adults who consumed either beetroot juice (BRJ, 140 mL; ∼8 mmol NO₃⁻) or a control beverage (prune juice; CON, 166 mL; <0.01 mmol NO₃⁻) following a random crossover study design. Two hours after consuming the allocated beverage, each participant rested for 4 min and then performed a continuous isometric forearm exercise (forearm exercise) using the right arm at 45 % of their maximal voluntary constriction for 1.5 min, followed by a 2-min recovery period with arterial occlusion of the exercising arm to activate the muscle metaboreflex only. Mean arterial pressure (MAP), heart rate (HR), and the cross-sectional area of the superficial vein in the left non-exercising arm (CSA_vein) were measured. BRJ intake increased the plasma NO₃⁻ concentration (p < 0.05). All resting parameters were similar with CON and BRJ. MAP and HR increased and the CSA_vein decreased with exercise (p < 0.05), and these changes were maintained during recovery, except for HR. The increase in MAP was lower in the BRJ group than in the CON group (p < 0.05), although the magnitude of the CSA_vein decrease did not differ between the groups. These findings suggest that, in a single conduit vein, the increasing NO precursors by BRJ intake does not alter either the venous vascular tone at rest or the sympathetic venoconstriction during forearm exercise or during the activation of muscle metaboreceptors.

补充硝酸盐（NO3-）后增加一氧化氮（NO）活性和/或NO前体水平对静脉血管控制的影响尚不清楚。我们研究了急性硝酸盐补充对静息和交感神经兴奋（如静态运动和肌肉代谢反射）时单个导管静脉血管反应的影响。参与者是15名健康的年轻人，他们要么喝甜菜根汁(BRJ， 140毫升；~ 8 mmol NO3-)或对照饮料(西梅汁；CON, 166 mL；< 0.01 mmol NO3-)，随机交叉研究设计。在饮用分配的饮料2小时后，每个参与者休息4分钟，然后使用右臂进行持续的等长前臂运动（前臂运动），以其最大自主收缩的45%进行1.5分钟，随后是2分钟的恢复期，运动臂动脉闭塞，仅激活肌肉代谢反射。测量左非运动臂平均动脉压（MAP）、心率（HR）和浅静脉横截面积（CSAvein）。摄入BRJ使血浆硝酸盐浓度升高（p < 0.05）。所有静息参数与CON和BRJ相似。运动后MAP、HR升高，csavin降低（p < 0.05），除HR外，恢复期间均保持上述变化。BRJ组MAP的增加低于CON组（p < 0.05），但csavin减少的幅度在两组之间没有差异。这些发现表明，在单个导管静脉中，BRJ摄入增加的NO前体不会改变静息时静脉血管张力，也不会改变前臂运动或肌肉代谢受体激活时交感静脉收缩。

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引用次数: 0

The Nitric Oxide Society and the Nitric Oxide Journal: Returning to our foundation to shape the future of redox biology 一氧化氮学会和一氧化氮杂志：回到我们的基础，塑造氧化还原生物学的未来。

IF 3.2 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Nitric oxide : biology and chemistry

Pub Date : 2025-05-21 DOI: 10.1016/j.niox.2025.05.005

Miriam M. Cortese-Krott , Lorenzo Berra , Nathan S. Bryan , Mattias Carlström , Sharon Glynn , Adrian Hobbs , Katrina M. Miranda , Hozumi Motohashi , Motohiro Nishida , Ciara E. O'Neill , Jesus Tejero

The Nitric Oxide Society and its journal, Nitric Oxide: Biology and Chemistry, are renewing their partnership to reconnect with their founding mission—supporting a dynamic, global redox community. Established together in 1996, the Society and Journal now return to a shared path, aligning efforts to empower young scientists, promote rigorous publishing, and foster open, international collaboration. As nitric oxide and related species move to the forefront of biomedical research and personalized medicine, this relaunch is less a restart than a return to form—focused, inclusive, and forward-looking.

一氧化氮学会和它的期刊《一氧化氮：生物学和化学》正在更新他们的伙伴关系，以重新连接他们的创始使命——支持一个充满活力的全球氧化还原社区。该学会和《期刊》于1996年共同成立，现在回到了共同的道路上，共同努力赋予年轻科学家权力，促进严谨的出版，促进开放的国际合作。随着一氧化氮和相关物种走向生物医学研究和个性化医疗的前沿，这次重新启动与其说是重新开始，不如说是回归到以形式为中心、包容和前瞻性。

引用次数: 0

The association between serum and urinary nitric oxide metabolites and fatty liver index: a population-based study 血清和尿一氧化氮代谢物与脂肪肝指数之间的关系：一项基于人群的研究。

IF 3.2 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Nitric oxide : biology and chemistry

Pub Date : 2025-05-20 DOI: 10.1016/j.niox.2025.05.004

Zahra Bahadoran , Fereidoun Azizi , Asghar Ghasemi

Background and aim

We investigated the association between fasting serum and urinary nitric oxide metabolite (NOx) levels and fatty liver index (FLI), a non-invasive surrogate of non-alcoholic fatty liver disease (NAFLD) and liver steatosis. Method: This cross-sectional study included 598 adults (aged≥18 years, 48.6 % men) who participated in the Tehran Lipid and Glucose Study (2015–2017). Serum and urine NOx concentrations were quantified using a spectrophotometric method following the Griess reaction. FLI values were calculated using γ-glutamyl transferase, triglycerides, body mass index, and waist circumference. The associations between urinary and serum NOx-to-creatinine (Cr) ratio [either as a categorical variable, i.e., tertiles, or as a continuous variable, i.e., per 1 SD) with NAFLD (i.e., FLI≥60) were assessed using multivariable-adjusted binary logistic regression.

Results

The study participants' mean (SD) age was 42.5 ± 14.6 y. The mean (SD) of serum and urinary NOx was 37.5 ± 16.7 and 1310 ± 751 μmol/L, respectively. The mean (SD) of FLI was 43.3 ± 30.2, and the prevalence of NAFLD was 32.4 %. Serum NOx-to-Cr ratio was not associated with the chance of having NAFLD (OR = 1.66, 95 % CI = 0.98–2.82; P value = 0.058). Higher urinary NOx-to-Cr ratio was significantly associated with a reduced probability of NAFLD (OR = 0.61, 95 % CI = 0.38–0.95, and OR = 0.54, 95 % CI = 0.34–0.87, in the second and third tertiles).

Conclusion

Higher dietary nitrate (NO₃) intake, indicated by increased urinary NOx-to-Cr ratio, is associated with a reduced probability of NAFLD, highlighting the potential role of dietary NO₃ in liver health.

背景和目的：我们研究了空腹血清和尿一氧化氮代谢物（NOx）水平与脂肪肝指数（FLI）之间的关系，FLI是一种非酒精性脂肪性肝病（NAFLD）和肝脏脂肪变性的无创替代指标。方法：本横断面研究纳入了参加德黑兰脂质和葡萄糖研究（2015-2017）的598名成年人（年龄≥18岁，48.6%为男性）。在Griess反应后，用分光光度法定量测定血清和尿液NOx浓度。使用γ-谷氨酰转移酶、甘油三酯、体重指数和腰围计算FLI值。尿和血清nox -肌酐（Cr）比值[作为一个分类变量，即分位数，或作为一个连续变量，即每1 SD)与NAFLD（即FLI≥60）之间的关系使用多变量调整的二元逻辑回归进行评估。结果：研究对象的平均（SD）年龄为42.5±14.6岁，血清和尿液NOx的平均（SD）分别为37.5±16.7和1310±751 μmol/L。FLI的平均值（SD）为43.3±30.2，NAFLD患病率为32.4%。血清nox - cr比值与NAFLD发生率无相关性(OR=1.66, 95% CI=0.98-2.82；P值= 0.058)。较高的尿nox - cr比值与NAFLD发生率降低显著相关（OR=0.61, 95% CI=0.38-0.95， OR=0.54, 95% CI=0.34-0.87，在第二和第三分位）。结论：较高的膳食硝酸盐（NO3）摄入量（表现为尿nox / cr比值增加）与NAFLD的可能性降低相关，突出了膳食NO3在肝脏健康中的潜在作用。

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引用次数: 0

Acute effects of l-arginine intake on heart rate variability after a submaximal exercise test in healthy men: randomized clinical trial 健康男性次最大运动试验后左旋精氨酸摄入对心率变异性的急性影响：随机临床试验

IF 3.2 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Nitric oxide : biology and chemistry

Pub Date : 2025-05-17 DOI: 10.1016/j.niox.2025.05.003

Andrey Alves Porto , Luana Almeida Gonzaga , Rayana Loch Gomes , Bruno M. Candeloro , Rodrigo Daminello Raimundo , Luiz Carlos Marques Vanderlei , Vitor Engrácia Valenti

l-arginine (L-ARG) is a semi-essential amino acid and a precursor for nitric oxide (NO) synthesis via endothelial nitric oxide synthase (eNOS). While NO has been implicated in autonomic modulation and vascular function, the effects of acute L-ARG intake on heart rate variability (HRV) after exercise remain unclear. This study aimed to assess the acute effects of L-ARG supplementation on HRV and cardiovascular recovery following submaximal aerobic exercise in healthy men. In a triple-blind, randomized, placebo-controlled, crossover trial, 37 physically active males (18–30 years) underwent two treadmill exercise protocols: one following ingestion of 3 g of L-ARG and the other with placebo. HRV and cardiovascular parameters (heart rate, systolic and diastolic blood pressure) were measured at baseline and across 20 min of post-exercise recovery. Time- and frequency-domain HRV indices were analyzed using validated algorithms. Two-way repeated measures ANOVA and post-hoc tests were applied (p < 0.05). Significant time effects were observed for HRV and cardiovascular variables across both protocols (p < 0.001), indicating physiological recovery. However, no statistically significant differences were found between the L-ARG and placebo conditions for any HRV or hemodynamic outcome. A trend toward faster vagal reactivation (rMSSD) was observed with L-ARG, but effect sizes were small and not clinically relevant. Acute supplementation with 3 g of l-arginine did not significantly influence HRV or cardiovascular recovery following submaximal aerobic exercise in healthy young men. These findings suggest limited autonomic effects of L-ARG in populations with high baseline HRV.

l-精氨酸（L-ARG）是一种半必需氨基酸，是通过内皮型一氧化氮合酶（eNOS）合成一氧化氮（NO）的前体。虽然一氧化氮与自主神经调节和血管功能有关，但急性左旋arg摄入对运动后心率变异性（HRV）的影响尚不清楚。本研究旨在评估补充L-ARG对健康男性次最大有氧运动后HRV和心血管恢复的急性影响。在一项三盲、随机、安慰剂对照、交叉试验中，37名身体活跃的男性（18-30岁）接受了两种跑步机锻炼方案：一种是摄入3g L-ARG，另一种是安慰剂。在基线和运动后恢复20分钟内测量HRV和心血管参数（心率、收缩压和舒张压）。采用验证算法对时域和频域HRV指标进行了分析。采用双向重复测量方差分析和事后检验(p <；0.05)。在两种方案中，HRV和心血管变量观察到显著的时间效应(p <；0.001)，表明生理恢复。然而，在任何HRV或血流动力学结果方面，L-ARG和安慰剂组之间没有统计学上的显著差异。L-ARG有更快的迷走神经再激活（rMSSD）的趋势，但效应量很小，与临床无关。在健康的年轻男性中，急性补充3g l-精氨酸对次最大有氧运动后HRV或心血管恢复没有显著影响。这些发现表明，在基线HRV高的人群中，L-ARG的自主作用有限。

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引用次数: 0

Nitric oxide based therapies for peripheral artery disease: Evidence and opportunities 基于一氧化氮的外周动脉疾病治疗：证据和机会。

IF 3.2 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Nitric oxide : biology and chemistry

Pub Date : 2025-05-16 DOI: 10.1016/j.niox.2025.05.002

Mary M. McDermott , Daniel B. Kim-Shapiro , Jason D. Allen

引用次数: 0

Generation of high-concentration medical-grade nitric oxide via air-sourced pulsed arc discharge 空气源脉冲电弧放电产生高浓度医用级一氧化氮

IF 3.2 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Nitric oxide : biology and chemistry

Pub Date : 2025-05-12 DOI: 10.1016/j.niox.2025.05.001

Weiming Ni , Haixiao Wu , Guangqing Liu , Jun Li , Yegang Lu

Inhaled nitric oxide (NO) is a selective pulmonary vasodilator that has been shown to be effective in treating pulmonary hypertension in both adults and children with respiratory and heart failure. The most common method for delivering NO in clinical practice relies on compressed high-concentration NO gas stored in cylinders. This NO gas is typically synthesized through chemical methods and stored in high-concentration form within gas cylinders, making NO therapy equipment bulky and expensive. Moreover, chemical synthesis requires specific materials, and the production and storage of NO are two separate steps, both of which are complex and costly. These factors limit the widespread adoption of NO therapy. To address the issues associated with high-concentration NO cylinder therapy, this study explores a method for generating NO gas through the ionization of air via pulsed arc discharge, and further investigates strategies to increase its concentration. A portable NO generator is designed to produce high-concentration NO gas on-demand, providing a potential alternative to cylinder storage. The results show that the four-electrode NO generator can quickly and safely produce 545.2 ± 10 ppm of NO and reduce the co-production of other gases. The pulsed arc NO generator designed in this study generates sufficiently high concentrations of NO gas to replace cylinder storage with lightweight, portable, and capable of immediate on-demand generation. The generator produces NO gas from ambient air, thus significantly reducing the cost of inhaled NO therapy and paving the way for wider application of this therapy.

吸入型一氧化氮（NO）是一种选择性肺血管扩张剂，已被证明可有效治疗呼吸和心力衰竭的成人和儿童肺动脉高压。在临床实践中，最常用的方法是将压缩的高浓度NO气体储存在钢瓶中。这种NO气体通常是通过化学方法合成的，并以高浓度的形式储存在气瓶中，这使得NO治疗设备体积庞大且昂贵。此外，化学合成需要特定的材料，而NO的生产和储存是两个独立的步骤，两者都是复杂和昂贵的。这些因素限制了NO疗法的广泛应用。为了解决高浓度NO气瓶治疗的相关问题，本研究探索了一种通过脉冲电弧放电电离空气产生NO气体的方法，并进一步研究了提高NO浓度的策略。便携式NO发生器设计用于按需生产高浓度NO气体，为钢瓶存储提供了一种潜在的替代方案。结果表明，四电极NO发生器能够快速、安全地产生545.2±10 ppm的NO，并减少了其他气体的共生。本研究设计的脉冲电弧NO发生器可产生足够高浓度的NO气体，以轻便、便携、可即时按需生成的方式取代钢瓶储存。该发生器从环境空气中产生NO气体，从而大大降低了吸入NO疗法的成本，为该疗法的更广泛应用铺平了道路。

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