Maize (Zea mays) is a high-nitrogen (N)-demanding crop potentially contributing to nitrate contamination and emissions of nitrous oxide. The N fertilization is generally split between sowing time and the V6 stage. The right split N rate to apply at V6 and minimize environmental damage is challenging. Our objectives were to (1) predict maize response to added N at V6 using machine learning (ML) models; and (2) cross-check model outcomes by independent on-farm trials. We assembled 461 N trials conducted in Eastern Canada between 1992 and 2022. The dataset to predict grain yield comprised N dosage, weekly precipitations and corn heat units, seeding date, previous crop, tillage practice, soil series, soil texture, organic matter content, and pH. Random forest and XGBoost predicted grain yield accurately at the V6 stage (R2 = 0.78–0.80; RSME and MAE = 1.22–1.29 and 0.96–0.98 Mg ha−1, respectively). Model accuracy up to the V6 stage was comparable to that of the full-season prediction. The response patterns simulated by varying the N doses showed that grain yield started to plateau at 125–150 kg total N ha−1 in eight out of ten on-farm trials conducted independently. There was great potential for economic and environmental gains from ML-assisted N fertilization.
{"title":"Simulating Maize Response to Split-Nitrogen Fertilization Using Easy-to-Collect Local Features","authors":"Léon Etienne Parent, Gabriel Deslauriers","doi":"10.3390/nitrogen4040024","DOIUrl":"https://doi.org/10.3390/nitrogen4040024","url":null,"abstract":"Maize (Zea mays) is a high-nitrogen (N)-demanding crop potentially contributing to nitrate contamination and emissions of nitrous oxide. The N fertilization is generally split between sowing time and the V6 stage. The right split N rate to apply at V6 and minimize environmental damage is challenging. Our objectives were to (1) predict maize response to added N at V6 using machine learning (ML) models; and (2) cross-check model outcomes by independent on-farm trials. We assembled 461 N trials conducted in Eastern Canada between 1992 and 2022. The dataset to predict grain yield comprised N dosage, weekly precipitations and corn heat units, seeding date, previous crop, tillage practice, soil series, soil texture, organic matter content, and pH. Random forest and XGBoost predicted grain yield accurately at the V6 stage (R2 = 0.78–0.80; RSME and MAE = 1.22–1.29 and 0.96–0.98 Mg ha−1, respectively). Model accuracy up to the V6 stage was comparable to that of the full-season prediction. The response patterns simulated by varying the N doses showed that grain yield started to plateau at 125–150 kg total N ha−1 in eight out of ten on-farm trials conducted independently. There was great potential for economic and environmental gains from ML-assisted N fertilization.","PeriodicalId":19365,"journal":{"name":"Nitrogen","volume":" 12","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135241610","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Charlene N. Kelly, Elizabeth A. Matejczyk, Emma G. Fox-Fogle, Jason A. Hubbart, Timothy P. Driscoll
Abundance of soil microbial nitrogen (N) cycling genes responsible for nitrification, denitrification, and nitrous oxide reduction may vary with tree species and N inputs, and these variables may be used to predict or mediate nitrate (NO3−) and nitrous oxide (N2O) from soil. Nitrification and denitrification rates have also been linked to tree mycorrhizal associations, as soil beneath species associated with arbuscular mycorrhiza (AM) shows greater nitrification rates than species forming ectomycorrhizal (ECM) associations. In this study, we integrated N microbial functional gene abundance in the soil influenced by six tree species in two sub-catchments receiving either high or low N inputs. The soils beneath the two ECM-associated tree species and the four AM-associated tree species were analyzed for inorganic N content and potential N2O flux and microbial gene abundance (nirK and nosZ) was quantified using qPCR techniques. Other parameters measured include soil pH, moisture, and organic matter. We determined that tree species influence NO3− and N2O production in riparian soils, particularly under high N enrichment. The soil beneath black cherry had the lowest pH, NO3− concentration, potential N2O production, and OM, though this result did not occur in the low N catchment. The strongest predictors of soil NO3− and N2O across the study sites were N enrichment and pH, respectively. These results provide a framework for species selection in managed riparian zones to minimize NO3− and N2O production and improve riparian function.
{"title":"Tree Species Influence Nitrate and Nitrous Oxide Production in Forested Riparian Soils","authors":"Charlene N. Kelly, Elizabeth A. Matejczyk, Emma G. Fox-Fogle, Jason A. Hubbart, Timothy P. Driscoll","doi":"10.3390/nitrogen4040023","DOIUrl":"https://doi.org/10.3390/nitrogen4040023","url":null,"abstract":"Abundance of soil microbial nitrogen (N) cycling genes responsible for nitrification, denitrification, and nitrous oxide reduction may vary with tree species and N inputs, and these variables may be used to predict or mediate nitrate (NO3−) and nitrous oxide (N2O) from soil. Nitrification and denitrification rates have also been linked to tree mycorrhizal associations, as soil beneath species associated with arbuscular mycorrhiza (AM) shows greater nitrification rates than species forming ectomycorrhizal (ECM) associations. In this study, we integrated N microbial functional gene abundance in the soil influenced by six tree species in two sub-catchments receiving either high or low N inputs. The soils beneath the two ECM-associated tree species and the four AM-associated tree species were analyzed for inorganic N content and potential N2O flux and microbial gene abundance (nirK and nosZ) was quantified using qPCR techniques. Other parameters measured include soil pH, moisture, and organic matter. We determined that tree species influence NO3− and N2O production in riparian soils, particularly under high N enrichment. The soil beneath black cherry had the lowest pH, NO3− concentration, potential N2O production, and OM, though this result did not occur in the low N catchment. The strongest predictors of soil NO3− and N2O across the study sites were N enrichment and pH, respectively. These results provide a framework for species selection in managed riparian zones to minimize NO3− and N2O production and improve riparian function.","PeriodicalId":19365,"journal":{"name":"Nitrogen","volume":"22 2","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135590187","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. G. D. S. Brochado, Laryssa Barbosa Xavier da Silva, A. Lima, Yure Marin Guidi, Kassio Ferreira Mendes
The interaction of herbicides in the nitrogen cycle and their consequences on soil health and agricultural production are essential topics in agronomic research. In this systematic review article, we have synthesized recent studies on this subject. The results revealed that the indiscriminate use of herbicides can have negative effects on vital processes in the nitrogen cycle, such as reduced enzymatic activity and microbial respiration. Moreover, herbicides alter the soil microbial composition, affecting nitrogen cycling-related activities. Symbiotic nitrogen fixation is also impaired, resulting in a reduction in the population of nitrogen-fixing bacteria and a decrease in the availability of this nutrient in the soil. These effects compromise soil fertility and the release of nitrogen to plants. Therefore, sustainable agricultural practices must be adopted, considering nitrogen cycling efficiency and the preservation of soil and natural resources. This understanding is crucial for guiding appropriate management strategies aimed at minimizing the negative effects of herbicides on the nitrogen cycle and ensuring soil health and agricultural productivity.
{"title":"Herbicides versus Nitrogen Cycle: Assessing the Trade-Offs for Soil Integrity and Crop Yield—An In-Depth Systematic Review","authors":"M. G. D. S. Brochado, Laryssa Barbosa Xavier da Silva, A. Lima, Yure Marin Guidi, Kassio Ferreira Mendes","doi":"10.3390/nitrogen4030022","DOIUrl":"https://doi.org/10.3390/nitrogen4030022","url":null,"abstract":"The interaction of herbicides in the nitrogen cycle and their consequences on soil health and agricultural production are essential topics in agronomic research. In this systematic review article, we have synthesized recent studies on this subject. The results revealed that the indiscriminate use of herbicides can have negative effects on vital processes in the nitrogen cycle, such as reduced enzymatic activity and microbial respiration. Moreover, herbicides alter the soil microbial composition, affecting nitrogen cycling-related activities. Symbiotic nitrogen fixation is also impaired, resulting in a reduction in the population of nitrogen-fixing bacteria and a decrease in the availability of this nutrient in the soil. These effects compromise soil fertility and the release of nitrogen to plants. Therefore, sustainable agricultural practices must be adopted, considering nitrogen cycling efficiency and the preservation of soil and natural resources. This understanding is crucial for guiding appropriate management strategies aimed at minimizing the negative effects of herbicides on the nitrogen cycle and ensuring soil health and agricultural productivity.","PeriodicalId":19365,"journal":{"name":"Nitrogen","volume":"64 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85710584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hanna Ibiapina de Jesus, A. D. da Silva, K. Cassity‐Duffey, T. Coolong
Efficient nitrogen (N) fertilizer applications in onion (Allium cepa L.) can reduce input costs and improve fertilizer-use efficiency, while maintaining high yields and quality. Understanding the N requirements of onion at different growth stages is necessary to enhance fertilizer N-use efficiency (FNUE). In a two-year study (2021 and 2022), the FNUE of onions was determined at five stages of development (at transplant, vegetative growth, bulb initiation, bulb swelling and bulb maturation). The FNUE was estimated by substituting a conventional N fertilizer (ammonium nitrate) with a 5% enriched 15N ammonium nitrate at a rate of 22.4 kg·ha−1 N, at one of five application times corresponding to a stage of development. All onions received a season total of 112 kg·ha−1 N. Marketable yield of onions was significantly greater in 2022 compared to 2021 and FNUE was affected by application timing in both years. In 2021, the FNUE at transplant was 8.9%, increasing to 26.4% and 35.28% at vegetative growth and bulb initiation stages, respectively. At bulb swelling and bulb maturation stages, FNUE was greater than 95%. In 2022, the FNUE at transplant was 25.2%. This increased to 75.7% and 103% at vegetative growth and bulb initiation stages, respectively. Results suggest that the application of fertilizer N at transplant is inefficient due to limited plant uptake ability, while N applications during bulb initiation and swelling were the most efficient.
{"title":"Estimating Fertilizer Nitrogen-Use Efficiency in Transplanted Short-Day Onion","authors":"Hanna Ibiapina de Jesus, A. D. da Silva, K. Cassity‐Duffey, T. Coolong","doi":"10.3390/nitrogen4030021","DOIUrl":"https://doi.org/10.3390/nitrogen4030021","url":null,"abstract":"Efficient nitrogen (N) fertilizer applications in onion (Allium cepa L.) can reduce input costs and improve fertilizer-use efficiency, while maintaining high yields and quality. Understanding the N requirements of onion at different growth stages is necessary to enhance fertilizer N-use efficiency (FNUE). In a two-year study (2021 and 2022), the FNUE of onions was determined at five stages of development (at transplant, vegetative growth, bulb initiation, bulb swelling and bulb maturation). The FNUE was estimated by substituting a conventional N fertilizer (ammonium nitrate) with a 5% enriched 15N ammonium nitrate at a rate of 22.4 kg·ha−1 N, at one of five application times corresponding to a stage of development. All onions received a season total of 112 kg·ha−1 N. Marketable yield of onions was significantly greater in 2022 compared to 2021 and FNUE was affected by application timing in both years. In 2021, the FNUE at transplant was 8.9%, increasing to 26.4% and 35.28% at vegetative growth and bulb initiation stages, respectively. At bulb swelling and bulb maturation stages, FNUE was greater than 95%. In 2022, the FNUE at transplant was 25.2%. This increased to 75.7% and 103% at vegetative growth and bulb initiation stages, respectively. Results suggest that the application of fertilizer N at transplant is inefficient due to limited plant uptake ability, while N applications during bulb initiation and swelling were the most efficient.","PeriodicalId":19365,"journal":{"name":"Nitrogen","volume":"65 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73716121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The determination of the endodormancy release and the beginning of ontogenetic development is a challenge, because these are non-observable stages. Changes in protein activity are important aspects of signal transduction. The conversion of threonine to 2-oxobutanoate is the first step towards isoleucine (Ile) biosynthesis, which promote growth and development. The reaction is catalyzed by threonine deaminase/dehydratase (TD). This study on TD activity was conducted at the experimental sweet cherry orchard at Berlin-Dahlem. Fresh (FW), dry weight (DW), water content (WC), and the specific TD activity for the cherry cultivars Summit, Karina and Regina were conducted from flower bud samples between October and April. The content of asparagine (Asn), aspartic acid (Asp), Ile, and valine (Val) were exemplarily shown for Summit. In buds of Summit and Karina, the TD activity was one week after the beginning of the ontogenetic development (t1*), significantly higher compared to samplings during endo- and ecodormancy. Such “peak” activity did not occur in the buds of Regina; TD tended for a longer time (day of year, DOY 6–48) to a higher activity, compared to the time DOY 287–350. For the date “one week after t1*”, the upregulation of TD, the markedly increase of the Ile and Val content, and the increase of the water content in the buds, all this enzymatically confirms the estimated start of the ontogenetic development (t1*) in sweet cherry buds.
内休眠释放和个体发育开始的确定是一个挑战，因为这些都是不可观察的阶段。蛋白质活性的变化是信号转导的重要方面。苏氨酸转化为2-氧丁酸盐是异亮氨酸(Ile)生物合成的第一步，异亮氨酸促进生长发育。该反应由苏氨酸脱氨酶/脱水酶(TD)催化。本研究在柏林达勒姆甜樱桃园进行了TD活性的研究。对Summit、Karina和Regina三个樱桃品种的花蕾样品进行了新鲜(FW)、干重(DW)、含水量(WC)和特定TD活性的测定。天冬酰胺(Asn)、天冬氨酸(Asp)、Ile和缬氨酸(Val)的含量以峰顶为例。在Summit和Karina芽中，TD活性在个体发育开始后一周(t1*)，显著高于endo- and - eco - mancy期间的样品。这种“高峰”活动在里贾纳芽中没有发生;与DOY 287-350时间相比，TD在较长的时间(一年中的一天，DOY 6-48)趋向于较高的活动。在“t1*后一周”，TD的上调，Ile和Val含量的显著增加，以及芽中含水量的增加，这些都从酶的角度证实了甜樱桃芽中个体发育的开始(t1*)。
{"title":"The Role of Threonine Deaminase/Dehydratase in Winter Dormancy in Sweet Cherry Buds","authors":"K. Götz, Chibueze Ene, J. Fettke, F. Chmielewski","doi":"10.3390/nitrogen4030020","DOIUrl":"https://doi.org/10.3390/nitrogen4030020","url":null,"abstract":"The determination of the endodormancy release and the beginning of ontogenetic development is a challenge, because these are non-observable stages. Changes in protein activity are important aspects of signal transduction. The conversion of threonine to 2-oxobutanoate is the first step towards isoleucine (Ile) biosynthesis, which promote growth and development. The reaction is catalyzed by threonine deaminase/dehydratase (TD). This study on TD activity was conducted at the experimental sweet cherry orchard at Berlin-Dahlem. Fresh (FW), dry weight (DW), water content (WC), and the specific TD activity for the cherry cultivars Summit, Karina and Regina were conducted from flower bud samples between October and April. The content of asparagine (Asn), aspartic acid (Asp), Ile, and valine (Val) were exemplarily shown for Summit. In buds of Summit and Karina, the TD activity was one week after the beginning of the ontogenetic development (t1*), significantly higher compared to samplings during endo- and ecodormancy. Such “peak” activity did not occur in the buds of Regina; TD tended for a longer time (day of year, DOY 6–48) to a higher activity, compared to the time DOY 287–350. For the date “one week after t1*”, the upregulation of TD, the markedly increase of the Ile and Val content, and the increase of the water content in the buds, all this enzymatically confirms the estimated start of the ontogenetic development (t1*) in sweet cherry buds.","PeriodicalId":19365,"journal":{"name":"Nitrogen","volume":"34 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87851955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. González-Lázaro, L. Martínez-Lapuente, T. Garde-Cerdán, Mikel Landín Ross-Magahy, Lesly L. Torres-Díaz, E. Pérez-Álvarez, Z. Guadalupe, B. Ayestarán
Polysaccharides are the main group of macromolecules in wines. Climate change is a major problem for viticulturists as it leads to the production of unbalanced grapes. This is attributed to a mismatch between the technological maturity and phenolic maturity of grapes, which can negatively impact the production of high quality wines. To mitigate this effect, biostimulants can be applied to grapevines. For the first time in the literature, this work studied the foliar application of methyl jasmonate plus urea (MeJ + Ur) on the vineyard and its effect on the monosaccharide and polysaccharide composition of Tempranillo grapes and wines over two consecutive seasons. To achieve this, the extraction and precipitation of polysaccharides was conducted, and the identification and quantitation of monosaccharides was performed via GC–MS. The effect of MeJ + Ur foliar treatment in both the grapes and wines was season-dependent. The MeJ + Ur treatment had a slight impact on the monosaccharide composition of the grapes and also demonstrated a small effect on the wines. Multifactor and discriminant analysis revealed that the season had a greater influence on the monosaccharide and polysaccharide composition of grapes and wines compared to the influence of MeJ + Ur treatment. Interestingly, the MeJ + Ur-treated wines exhibited a higher sensory evaluation than the control wines in the second vintage. To gain further insights into the effect of MeJ + Ur foliar application on the monosaccharide and polysaccharide composition of grapes and wines, further investigations should be conducted.
{"title":"Effect of Methyl Jasmonate Plus Urea Foliar Application on the Polysaccharide and Monosaccharide Composition of Tempranillo Grapes and Wines and on the Wine’s Quality","authors":"M. González-Lázaro, L. Martínez-Lapuente, T. Garde-Cerdán, Mikel Landín Ross-Magahy, Lesly L. Torres-Díaz, E. Pérez-Álvarez, Z. Guadalupe, B. Ayestarán","doi":"10.3390/nitrogen4030019","DOIUrl":"https://doi.org/10.3390/nitrogen4030019","url":null,"abstract":"Polysaccharides are the main group of macromolecules in wines. Climate change is a major problem for viticulturists as it leads to the production of unbalanced grapes. This is attributed to a mismatch between the technological maturity and phenolic maturity of grapes, which can negatively impact the production of high quality wines. To mitigate this effect, biostimulants can be applied to grapevines. For the first time in the literature, this work studied the foliar application of methyl jasmonate plus urea (MeJ + Ur) on the vineyard and its effect on the monosaccharide and polysaccharide composition of Tempranillo grapes and wines over two consecutive seasons. To achieve this, the extraction and precipitation of polysaccharides was conducted, and the identification and quantitation of monosaccharides was performed via GC–MS. The effect of MeJ + Ur foliar treatment in both the grapes and wines was season-dependent. The MeJ + Ur treatment had a slight impact on the monosaccharide composition of the grapes and also demonstrated a small effect on the wines. Multifactor and discriminant analysis revealed that the season had a greater influence on the monosaccharide and polysaccharide composition of grapes and wines compared to the influence of MeJ + Ur treatment. Interestingly, the MeJ + Ur-treated wines exhibited a higher sensory evaluation than the control wines in the second vintage. To gain further insights into the effect of MeJ + Ur foliar application on the monosaccharide and polysaccharide composition of grapes and wines, further investigations should be conducted.","PeriodicalId":19365,"journal":{"name":"Nitrogen","volume":"26 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81307116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Nitrogen-fixing bacteria such as cyanobacteria have the capability to fix atmospheric nitrogen at ambient temperature and pressure, and intensive cultivation of cyanobacteria for fertilizer could lead to its use as an “environmentally friendly” replacement or supplement for nitrogen (N) fertilizer derived from the Haber–Bosch process. Prior research has focused on the use of N-fixing bacteria as a soil inoculum, and while this can improve crop yields, yield improvements are generally attributed to plant-growth-promoting substances produced by the bacteria, rather than to biological N fixation. The intensive cultivation of cyanobacteria in raceways or bioreactors can result in a fertilizer that provides N and organic carbon, as well as potentially similar growth-promoting substances observed in prior research work. On-farm or local production of cyanobacterial fertilizer could also circumvent infrastructure limitations, economic and geopolitical issues, and challenges in distribution and transport related to Haber–Bosch-derived N fertilizers. The use of cyanobacterial N fertilizer could have many agronomic and environmental advantages over N fertilizer derived from the Haber–Bosch process, but study of cyanobacteria as a replacement for other N fertilizers remains very limited. Scientific and practical challenges remain for this promising but as-yet unproven approach to maintaining or improving soil N fertility.
{"title":"Beyond Soil Inoculation: Cyanobacteria as a Fertilizer Replacement","authors":"M. Massey, Jessica G. Davis","doi":"10.3390/nitrogen4030018","DOIUrl":"https://doi.org/10.3390/nitrogen4030018","url":null,"abstract":"Nitrogen-fixing bacteria such as cyanobacteria have the capability to fix atmospheric nitrogen at ambient temperature and pressure, and intensive cultivation of cyanobacteria for fertilizer could lead to its use as an “environmentally friendly” replacement or supplement for nitrogen (N) fertilizer derived from the Haber–Bosch process. Prior research has focused on the use of N-fixing bacteria as a soil inoculum, and while this can improve crop yields, yield improvements are generally attributed to plant-growth-promoting substances produced by the bacteria, rather than to biological N fixation. The intensive cultivation of cyanobacteria in raceways or bioreactors can result in a fertilizer that provides N and organic carbon, as well as potentially similar growth-promoting substances observed in prior research work. On-farm or local production of cyanobacterial fertilizer could also circumvent infrastructure limitations, economic and geopolitical issues, and challenges in distribution and transport related to Haber–Bosch-derived N fertilizers. The use of cyanobacterial N fertilizer could have many agronomic and environmental advantages over N fertilizer derived from the Haber–Bosch process, but study of cyanobacteria as a replacement for other N fertilizers remains very limited. Scientific and practical challenges remain for this promising but as-yet unproven approach to maintaining or improving soil N fertility.","PeriodicalId":19365,"journal":{"name":"Nitrogen","volume":"11 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88638448","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Although NH4+ fertilization is known to acidify rhizosphere and enhance nutrient uptake, the effects on a nutrient-sufficient acidic soil amended with lime are not demonstrated. Thus, the influence of NH4+ fertilization of an unlimed and limed (3 g calcium carbonate per kg soil) acidic soil on the nutrient uptake and growth of maize was studied in comparison to NH4NO3 fertilization. The pH of limed rhizosphere soil was about two units higher than that of the unlimed soil. The maize plants were grown in pots under greenhouse conditions for about two months. The results showed that the pH of the NH4+-fertilized unlimed and limed soil was 0.54 and 0.15 units lower than the NH4NO3-fertilized soil. Liming negatively affected shoot and root dry matter production, whereas the NH4+-fertilized plants produced higher dry matter than the NH4NO3-fertilized plants, with significant difference of 28% in the limed soil only. Liming decreased Fe concentration in rhizosphere soil from 99 to 69 mg kg−1 and decreased plant-available Mn the most (71%), whereas the NH4+-fertilized unlimed and limed soil had 48% and 21% higher Mn concentration than the respective NH4NO3-fertilized soils. Similarly limed rhizosphere soil had 50% lower plant-available Zn concentration than the unlimed soil, and the NH4+-fertilized soil had an 8% higher Zn concentration than the NH4NO3-fertilized unlimed soil. The liming negatively affected P, K, Mn, and Zn concentrations and contents in maize shoot to a lower degree in the NH4+-fertilized soil, whereas the positive effect of NH4+ on the nutrient concentration and contents was vigorous in the unlimed soil than the limed soil. It is concluded that NH4+ fertilization could be beneficial in enhancing nutrient uptake and growth of maize in both acidic and alkaline soils, despite the higher inherent plant-available concentrations of the nutrient in soil.
虽然已知NH4+施肥可以酸化根际并增强养分吸收，但对石灰改良的营养充足的酸性土壤的影响尚未得到证实。在此基础上，研究了无石灰和有石灰(3 g碳酸钙/ kg土壤)酸性土壤中施用NH4+对玉米养分吸收和生长的影响，并与施用NH4NO3进行比较。石灰处理的根际土壤pH值比未石灰处理的根际土壤pH值高2个单位左右。这些玉米植株在温室条件下盆栽了大约两个月。结果表明:NH4+处理的土壤pH值比nh4no3处理的土壤pH值分别低0.54和0.15个单位;石灰化对土壤茎部和根系干物质产量有负向影响，而施用NH4+的植株干物质产量高于施用nh4no3的植株，仅石灰化土壤的干物质产量差异达28%。石灰使根际土壤铁浓度从99 ~ 69 mg kg - 1下降至69 mg kg - 1，使植物有效锰含量下降最多(71%)，而铵硝土和石灰土的锰浓度分别比铵硝土高48%和21%。同样，石灰根际土壤的植物有效锌浓度比未石灰土壤低50%，NH4+施肥的土壤锌浓度比nh4no3施肥的土壤高8%。石灰处理对NH4+施肥土壤中玉米茎部磷、钾、锰、锌浓度和含量的负向影响程度较低，而未石灰处理对玉米茎部磷、钾、锰、锌浓度和含量的正向影响较石灰处理强烈。综上所述，在酸性和碱性土壤中，施用NH4+有利于提高玉米的养分吸收和生长，尽管土壤中养分的内在植物有效浓度较高。
{"title":"Ammonium Fertilization Enhances Nutrient Uptake, Specifically Manganese and Zinc, and Growth of Maize in Unlimed and Limed Acidic Sandy Soil","authors":"A. Naeem, Philipp Deppermann, Karl H. Mühling","doi":"10.3390/nitrogen4020017","DOIUrl":"https://doi.org/10.3390/nitrogen4020017","url":null,"abstract":"Although NH4+ fertilization is known to acidify rhizosphere and enhance nutrient uptake, the effects on a nutrient-sufficient acidic soil amended with lime are not demonstrated. Thus, the influence of NH4+ fertilization of an unlimed and limed (3 g calcium carbonate per kg soil) acidic soil on the nutrient uptake and growth of maize was studied in comparison to NH4NO3 fertilization. The pH of limed rhizosphere soil was about two units higher than that of the unlimed soil. The maize plants were grown in pots under greenhouse conditions for about two months. The results showed that the pH of the NH4+-fertilized unlimed and limed soil was 0.54 and 0.15 units lower than the NH4NO3-fertilized soil. Liming negatively affected shoot and root dry matter production, whereas the NH4+-fertilized plants produced higher dry matter than the NH4NO3-fertilized plants, with significant difference of 28% in the limed soil only. Liming decreased Fe concentration in rhizosphere soil from 99 to 69 mg kg−1 and decreased plant-available Mn the most (71%), whereas the NH4+-fertilized unlimed and limed soil had 48% and 21% higher Mn concentration than the respective NH4NO3-fertilized soils. Similarly limed rhizosphere soil had 50% lower plant-available Zn concentration than the unlimed soil, and the NH4+-fertilized soil had an 8% higher Zn concentration than the NH4NO3-fertilized unlimed soil. The liming negatively affected P, K, Mn, and Zn concentrations and contents in maize shoot to a lower degree in the NH4+-fertilized soil, whereas the positive effect of NH4+ on the nutrient concentration and contents was vigorous in the unlimed soil than the limed soil. It is concluded that NH4+ fertilization could be beneficial in enhancing nutrient uptake and growth of maize in both acidic and alkaline soils, despite the higher inherent plant-available concentrations of the nutrient in soil.","PeriodicalId":19365,"journal":{"name":"Nitrogen","volume":"29 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85468679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jorge De La O-Sánchez, María A. Muñoz-Vargas, J. Palma, F. J. Corpas
In higher plants, hydrogen sulfide (H2S) is a recognized signaling molecule that performs multiple regulatory functions. The enzyme L-cysteine desulfhydrase (LCD) catalyzes the conversion of L-cysteine (L-Cys) to pyruvate and ammonium with the concomitant generation of H₂S, and it is considered one of the main sources of H2S in plants. Using non-denaturing polyacrylamide gel electrophoresis (PAGE) in combination with a specific assay for LCD activity, this study aims to identify the potential LCD isozymes in wild-type Arabidopsis thaliana seedlings of 16 days old grown under in vitro conditions, and to evaluate the potential impact of nitric oxide (NO) and H2S on these LCD isozymes. For this purpose, an Atnoa1 mutant characterized to have a low endogenous NO content as well as the exogenous application of H2S were used. Five LCD isozymes were detected, with LCD IV being the isozyme that has the highest activity. However, the LCD V activity was the only one that was positively modulated in the Atnoa1 mutants and by exogenous H2S. To our knowledge, this is the first report showing the different LCD isozymes present in Arabidopsis seedlings and how their activity is affected by NO and H2S content.
{"title":"Isoenzymatic Pattern of Hydrogen Sulfide (H2S)-Generating L-Cysteine Desulfhydrase (LCD) in Arabidopsis thaliana Seedlings: Effect of Nitric Oxide (NO) and H2S","authors":"Jorge De La O-Sánchez, María A. Muñoz-Vargas, J. Palma, F. J. Corpas","doi":"10.3390/nitrogen4020016","DOIUrl":"https://doi.org/10.3390/nitrogen4020016","url":null,"abstract":"In higher plants, hydrogen sulfide (H2S) is a recognized signaling molecule that performs multiple regulatory functions. The enzyme L-cysteine desulfhydrase (LCD) catalyzes the conversion of L-cysteine (L-Cys) to pyruvate and ammonium with the concomitant generation of H₂S, and it is considered one of the main sources of H2S in plants. Using non-denaturing polyacrylamide gel electrophoresis (PAGE) in combination with a specific assay for LCD activity, this study aims to identify the potential LCD isozymes in wild-type Arabidopsis thaliana seedlings of 16 days old grown under in vitro conditions, and to evaluate the potential impact of nitric oxide (NO) and H2S on these LCD isozymes. For this purpose, an Atnoa1 mutant characterized to have a low endogenous NO content as well as the exogenous application of H2S were used. Five LCD isozymes were detected, with LCD IV being the isozyme that has the highest activity. However, the LCD V activity was the only one that was positively modulated in the Atnoa1 mutants and by exogenous H2S. To our knowledge, this is the first report showing the different LCD isozymes present in Arabidopsis seedlings and how their activity is affected by NO and H2S content.","PeriodicalId":19365,"journal":{"name":"Nitrogen","volume":"104 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88969077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
G. Tortosa, S. Mesa, María J. Delgado, Carol V. Amaya-Gómez
The utilization of compost to enhance plant productivity and symbiotic nitrogen fixation (SNF) has been recognized as an effective alternative to synthetic nitrogen fertilizers. This environmentally sustainable method is readily accessible to farmers. This study investigated the effect of olive pomace (“alperujo”, AL) compost on the nodulation and SNF of soybeans (Glycine max L.) and their natural symbiont (Bradyrhizobium diazoefficiens). For that, soybean plants were subjected to several doses of AL compost under controlled greenhouse conditions. At the end of the experiment, the dry weight of plant biomass (aerial part and roots), the number and fresh weight of nodules, and nitrogen and leghaemoglobin contents were analyzed. The application of AL compost significantly improved soybean growth, as demonstrated by an increase in both plant biomass and height. Furthermore, nodular leghaemoglobin content and nitrogen content were found to be enhanced by the addition of AL compost (7 and 40%, respectively), indicating an increase in nodule effectiveness and symbiotic efficiency. Our results provide clear evidence of the synergetic effect of AL compost on the soybean-B. diazoefficiens association, probably due to AL-compost improved soybean roots development, and rhizospheric organic matter and nutrients assimilation by rhizobia.
利用堆肥提高植物生产力和共生固氮(SNF)已被公认为是合成氮肥的有效替代品。这种环境可持续的方法很容易为农民所用。本试验研究了橄榄渣(alperujo, AL)堆肥对大豆(Glycine max L.)及其天然共生体(重氮缓生根瘤菌)结瘤和SNF的影响。为此，大豆植株在可控的温室条件下施用了几种剂量的AL堆肥。试验结束时，测定植物生物量(地上部分和根系)干重、根瘤数和鲜重、氮和豆血红蛋白含量。施用AL堆肥对大豆生长有显著的促进作用，表现为植株生物量和株高的增加。此外，添加AL堆肥可提高根瘤血红蛋白含量和氮含量(分别为7%和40%)，表明根瘤效率和共生效率均有提高。本研究结果为AL堆肥对大豆- b的增效作用提供了明确的证据。重氮效率的关联，可能是由于铝堆肥促进了大豆根系的发育，以及根瘤菌对根际有机质和养分的同化。
{"title":"“Alperujo” Compost Improves Nodulation and Symbiotic Nitrogen Fixation of Soybean Inoculated with Bradyrhizobium diazoefficiens","authors":"G. Tortosa, S. Mesa, María J. Delgado, Carol V. Amaya-Gómez","doi":"10.3390/nitrogen4020015","DOIUrl":"https://doi.org/10.3390/nitrogen4020015","url":null,"abstract":"The utilization of compost to enhance plant productivity and symbiotic nitrogen fixation (SNF) has been recognized as an effective alternative to synthetic nitrogen fertilizers. This environmentally sustainable method is readily accessible to farmers. This study investigated the effect of olive pomace (“alperujo”, AL) compost on the nodulation and SNF of soybeans (Glycine max L.) and their natural symbiont (Bradyrhizobium diazoefficiens). For that, soybean plants were subjected to several doses of AL compost under controlled greenhouse conditions. At the end of the experiment, the dry weight of plant biomass (aerial part and roots), the number and fresh weight of nodules, and nitrogen and leghaemoglobin contents were analyzed. The application of AL compost significantly improved soybean growth, as demonstrated by an increase in both plant biomass and height. Furthermore, nodular leghaemoglobin content and nitrogen content were found to be enhanced by the addition of AL compost (7 and 40%, respectively), indicating an increase in nodule effectiveness and symbiotic efficiency. Our results provide clear evidence of the synergetic effect of AL compost on the soybean-B. diazoefficiens association, probably due to AL-compost improved soybean roots development, and rhizospheric organic matter and nutrients assimilation by rhizobia.","PeriodicalId":19365,"journal":{"name":"Nitrogen","volume":"20 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87074779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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