Pub Date : 2023-03-28DOI: 10.15517/ijds.2023.54635
Bahadır Ezmek, Osman Cumhur Sipahi
To evaluate the accuracy of complete arch scanning with multiple implant titanium scan bodies using laboratory scanners. A master model of an edentulous maxillary arch with 6 implants was fabricated. Titanium scan bodies were inserted into the model. Three laboratory scanners were used: D2000 (3Shape), Vinyl High Resolution (Smart Optics), and inEos X5 (Dentsply Sirona). The master model was consecutively scanned ten times using dental laboratory scanners (LS) without detaching and repositioning the scan bodies. Linear and angular accuracy between adjacent implants was measured using inspection software (Control X, Geomagic). The accuracy of the complete arch scans was calculated. Implant regions were defined as; parallel (R1: #24-26 and #16-14), angled (R2: #22-24 and #14-12), angled to occlusal plane (R3: #12-22), and cross-arch (R4: #16-26). The effect of LS and implant region on accuracy was compared using two-Way ANOVA (α=0.05). Significant greater linear distortion was noted in R4 (61.2±17.9µm) compared to R1 (23.4±15.5µm) and R2 (26±17.7µm) (p<0.01). Greater linear distortions were noted in R4 with D2000 (0.07±0.016 degrees) and Vinyl High Resolution (0.067±0.02 degrees) than inEos X5 (0.032±0.021 degrees) (p>0.05). Greater mean linear precisions were noted in R1 (9±8µm) and R3 (9.3±8.3µm) than R4 (12.6±10.3µm) (p<0.05). The highest linear precision was noted in D2000 (7.2±7.6µm) (p<0.05). The angular precision of D2000 (0.02±0.015 degrees) was the highest (p<0.01). The angular precision of R4 (0.036±0.018 degrees) was the lowest (p<0.01). This study revealed that the trueness was affected by the implant region and the precision was affected by both LS and implant region.
{"title":"Evaluation the Scanning Accuracy of Blue-Light Laboratory Scanners in Complete Edentulous Maxilla with Multiple Implants with Titanium Scan Bodies","authors":"Bahadır Ezmek, Osman Cumhur Sipahi","doi":"10.15517/ijds.2023.54635","DOIUrl":"https://doi.org/10.15517/ijds.2023.54635","url":null,"abstract":"To evaluate the accuracy of complete arch scanning with multiple implant titanium scan bodies using laboratory scanners. A master model of an edentulous maxillary arch with 6 implants was fabricated. Titanium scan bodies were inserted into the model. Three laboratory scanners were used: D2000 (3Shape), Vinyl High Resolution (Smart Optics), and inEos X5 (Dentsply Sirona). The master model was consecutively scanned ten times using dental laboratory scanners (LS) without detaching and repositioning the scan bodies. Linear and angular accuracy between adjacent implants was measured using inspection software (Control X, Geomagic). The accuracy of the complete arch scans was calculated. Implant regions were defined as; parallel (R1: #24-26 and #16-14), angled (R2: #22-24 and #14-12), angled to occlusal plane (R3: #12-22), and cross-arch (R4: #16-26). The effect of LS and implant region on accuracy was compared using two-Way ANOVA (α=0.05). Significant greater linear distortion was noted in R4 (61.2±17.9µm) compared to R1 (23.4±15.5µm) and R2 (26±17.7µm) (p<0.01). Greater linear distortions were noted in R4 with D2000 (0.07±0.016 degrees) and Vinyl High Resolution (0.067±0.02 degrees) than inEos X5 (0.032±0.021 degrees) (p>0.05). Greater mean linear precisions were noted in R1 (9±8µm) and R3 (9.3±8.3µm) than R4 (12.6±10.3µm) (p<0.05). The highest linear precision was noted in D2000 (7.2±7.6µm) (p<0.05). The angular precision of D2000 (0.02±0.015 degrees) was the highest (p<0.01). The angular precision of R4 (0.036±0.018 degrees) was the lowest (p<0.01). This study revealed that the trueness was affected by the implant region and the precision was affected by both LS and implant region.","PeriodicalId":19450,"journal":{"name":"Odovtos - International Journal of Dental Sciences","volume":"2 1","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83710314","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The use of ionizing radiation is legally regulated by health authorities due to the risk of harmful effects on patients and occupationally exposed personnel. In dentistry, the possible risk is carcinogenesis, being essential to know and apply radiological protection, in order to reduce this risk. There are few investigations on the level of knowledge of dental students in radiation protection, and none carried out in Central America. Questionnaires are a tool to determine this information; however, during their development, validation is essential, allowing the instrument to be used at different times or populations. The aim of this study was to develop and validate a questionnaire to assess the knowledge in radiation protection of undergraduate and postgraduate students in dentistry. A questionnaire on radiological protection was built based on literature and national legislation. For its validation, the pre-test was carried out in two stages: content review through the judgment of 6 experts, and application to a small sample of students. Once modified according to the pre-test, the questionnaire was applied to 62 students - who did not participate in the pre-test -. Content validity was assessed using Aiken's V coefficient in the first stage of the pre-test. Reproducibility was determined using the intraclass correlation coefficient (ICC), and reliability using Cronbach's alpha. An Aiken V coefficient of 0.95 was obtained, an ICC between 0.697 and 0.729 and a Cronbach's alpha of 0.727. The questionnaire developed is a valid instrument to evaluate the knowledge in radiological protection of undergraduate and postgraduate students in dentistry. This instrument will strengthen and improve the training of dental undergraduate and postgraduate students in radiological protection.
{"title":"Development and Validation of a Questionnaire on Radiation Protection in Dentistry","authors":"Lucía Barba Ramírez, Ana Cecilia Ruiz-Imbert, Deivi Cascante-Sequeira, Patricia Ovares Saballos, Alejandro Hidalgo Rivas","doi":"10.15517/ijds.2023.54539","DOIUrl":"https://doi.org/10.15517/ijds.2023.54539","url":null,"abstract":"The use of ionizing radiation is legally regulated by health authorities due to the risk of harmful effects on patients and occupationally exposed personnel. In dentistry, the possible risk is carcinogenesis, being essential to know and apply radiological protection, in order to reduce this risk. There are few investigations on the level of knowledge of dental students in radiation protection, and none carried out in Central America. Questionnaires are a tool to determine this information; however, during their development, validation is essential, allowing the instrument to be used at different times or populations. The aim of this study was to develop and validate a questionnaire to assess the knowledge in radiation protection of undergraduate and postgraduate students in dentistry. A questionnaire on radiological protection was built based on literature and national legislation. For its validation, the pre-test was carried out in two stages: content review through the judgment of 6 experts, and application to a small sample of students. Once modified according to the pre-test, the questionnaire was applied to 62 students - who did not participate in the pre-test -. Content validity was assessed using Aiken's V coefficient in the first stage of the pre-test. Reproducibility was determined using the intraclass correlation coefficient (ICC), and reliability using Cronbach's alpha. An Aiken V coefficient of 0.95 was obtained, an ICC between 0.697 and 0.729 and a Cronbach's alpha of 0.727. The questionnaire developed is a valid instrument to evaluate the knowledge in radiological protection of undergraduate and postgraduate students in dentistry. This instrument will strengthen and improve the training of dental undergraduate and postgraduate students in radiological protection.","PeriodicalId":19450,"journal":{"name":"Odovtos - International Journal of Dental Sciences","volume":"1 1","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86527970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-02-10DOI: 10.15517/ijds.2023.54119
P. P., Uma Maheswari T.N.
Electromyography (EMG) is used for the measurement of muscle activity to characterize the nature of muscle contraction in Oral submucous fibrosis (OSMF). Aim: To assess the efficacy of EMG in evaluating masseter muscle activity in the management of OSMF. This review identified 73 records from standard databases which were rigorously screened with eligibility criteria and 3 clinical studies were identified based on our inclusion criteria. The quality of included studies was assessed by the PEDro scale and data was synthesized with detailed characterization. The Risk of Bias assessment among studies was done using the ROBINS-I tool and a meta-analysis could not be done due to high clinical heterogeneity. Our result recommends that EMG be used as an objective prognosis assessment tool by quantifying the management of OSMF irrespective of the intervention applied. However, it is not to be considered the gold standard as of now with limited data pooled and needs to be further assessed with clinical trials. EMG can be advocated as a reliable adjunct assessment for measuring the interventional outcome of OSMF irrespective of treatment modalities.
{"title":"Assessment of Electromyography in Evaluating Masseter Muscle Activity in Oral Submucous Fibrosis with Different Treatment Modalities: a Systematic Review","authors":"P. P., Uma Maheswari T.N.","doi":"10.15517/ijds.2023.54119","DOIUrl":"https://doi.org/10.15517/ijds.2023.54119","url":null,"abstract":"Electromyography (EMG) is used for the measurement of muscle activity to characterize the nature of muscle contraction in Oral submucous fibrosis (OSMF). \u0000Aim: To assess the efficacy of EMG in evaluating masseter muscle activity in the management of OSMF. This review identified 73 records from standard databases which were rigorously screened with eligibility criteria and 3 clinical studies were identified based on our inclusion criteria. The quality of included studies was assessed by the PEDro scale and data was synthesized with detailed characterization. The Risk of Bias assessment among studies was done using the ROBINS-I tool and a meta-analysis could not be done due to high clinical heterogeneity. Our result recommends that EMG be used as an objective prognosis assessment tool by quantifying the management of OSMF irrespective of the intervention applied. However, it is not to be considered the gold standard as of now with limited data pooled and needs to be further assessed with clinical trials. EMG can be advocated as a reliable adjunct assessment for measuring the interventional outcome of OSMF irrespective of treatment modalities.","PeriodicalId":19450,"journal":{"name":"Odovtos - International Journal of Dental Sciences","volume":"31 1","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85836129","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-02-10DOI: 10.15517/ijds.2023.54120
Paola Manrique, A. Agurto, M. Guerrero
The purpose of this study was to compare the average distances from the root apices of the first molars, second molars, and second premolars to the mandibular canal according to sex in the Peruvian population using cone-beam computed tomography (CBCT). Eighty CBCT scans of Peruvian patients aged from 15-80 years were examined. After locating the mandibular canal, measurements of the vertical distances from the mandibular canal to the apices of the second premolars, as well as the first molars and second molars, were made. For the statistical analysis, Student’s t test was used for both paired and unpaired samples, with a significance level of p<0.05. On the right side, the second molar presented a mean distance of 3.99mm for males and 2.87mm for females, showing a significant difference (p<0.05). When compared bilaterally, no significant differences were found (p>0.05) between the distances from the apices of the second premolars and the first and second molars to the mandibular canal. However, for the second premolars and second molars on the left side, the values were higher, with averages of 5.52mm and 3.75mm, respectively.The mesial roots of the second molars were closer to the mandibular canal. In addition, women showed shorter distances than men.
{"title":"CBCT Study of Root Apices Distance to Mandibular Canal in Peruvian Population","authors":"Paola Manrique, A. Agurto, M. Guerrero","doi":"10.15517/ijds.2023.54120","DOIUrl":"https://doi.org/10.15517/ijds.2023.54120","url":null,"abstract":"The purpose of this study was to compare the average distances from the root apices of the first molars, second molars, and second premolars to the mandibular canal according to sex in the Peruvian population using cone-beam computed tomography (CBCT). Eighty CBCT scans of Peruvian patients aged from 15-80 years were examined. After locating the mandibular canal, measurements of the vertical distances from the mandibular canal to the apices of the second premolars, as well as the first molars and second molars, were made. For the statistical analysis, Student’s t test was used for both paired and unpaired samples, with a significance level of p<0.05. On the right side, the second molar presented a mean distance of 3.99mm for males and 2.87mm for females, showing a significant difference (p<0.05). When compared bilaterally, no significant differences were found (p>0.05) between the distances from the apices of the second premolars and the first and second molars to the mandibular canal. However, for the second premolars and second molars on the left side, the values were higher, with averages of 5.52mm and 3.75mm, respectively.The mesial roots of the second molars were closer to the mandibular canal. In addition, women showed shorter distances than men.","PeriodicalId":19450,"journal":{"name":"Odovtos - International Journal of Dental Sciences","volume":"30 1","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90444374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-02-10DOI: 10.15517/ijds.2023.54127
Natalia Solari, Leonardo Salas, S. Tabares, Carolina Rosella, M. M. Usín, A. Sembaj
We determine periodontal pathogens in periodontal pockets from pregnant women with periodontitis and associate it to the C reactive protein (CRP), nitrates, immunoglobulin A and G (Ig A and G), and myeloperoxidase (MPO) levels in saliva to identify some biomarkers as tools to predict the periodontal status from pregnant. The samples were obtained from periodontal pockets and saliva from 100 pregnant women (PW) and 50 non-pregnant women (NPW). Every patient was evaluated by: 1) probing depth (PD) and loss of clinical attachment level (CAL); 2) in saliva; CRP, MPO, Ig A and G) and nitrite concentrations, 3) in periodontal pockets: P.gingivalis, T.forsythia, T.denticola, P.intermedia, A.actinomycetemcomitans. InfoStat/P 2008 software was used with a p-value <0.05. Clinical parameters showed stages I and II of PD in both groups. P.intermedia and A.actinomycetemcomitans were observed only in periodontal pockets from PW. The CAL was higher in pregnant of the 3rd trimester than in the other stages and was associated with low levels of IgA and the presence of P.intermedia and T. forsythia in the same trimester. The levels of IgA in saliva would reflect the immunological situation in pregnant women. This could be used to monitor the immune status of the gingival tissues during pregnancy.
{"title":"Immunological and Bacteriological Monitoring of Periodontal Tissue in Pregnancy","authors":"Natalia Solari, Leonardo Salas, S. Tabares, Carolina Rosella, M. M. Usín, A. Sembaj","doi":"10.15517/ijds.2023.54127","DOIUrl":"https://doi.org/10.15517/ijds.2023.54127","url":null,"abstract":"We determine periodontal pathogens in periodontal pockets from pregnant women with periodontitis and associate it to the C reactive protein (CRP), nitrates, immunoglobulin A and G (Ig A and G), and myeloperoxidase (MPO) levels in saliva to identify some biomarkers as tools to predict the periodontal status from pregnant. The samples were obtained from periodontal pockets and saliva from 100 pregnant women (PW) and 50 non-pregnant women (NPW). Every patient was evaluated by: 1) probing depth (PD) and loss of clinical attachment level (CAL); 2) in saliva; CRP, MPO, Ig A and G) and nitrite concentrations, 3) in periodontal pockets: P.gingivalis, T.forsythia, T.denticola, P.intermedia, A.actinomycetemcomitans. InfoStat/P 2008 software was used with a p-value <0.05. Clinical parameters showed stages I and II of PD in both groups. P.intermedia and A.actinomycetemcomitans were observed only in periodontal pockets from PW. The CAL was higher in pregnant of the 3rd trimester than in the other stages and was associated with low levels of IgA and the presence of P.intermedia and T. forsythia in the same trimester. The levels of IgA in saliva would reflect the immunological situation in pregnant women. This could be used to monitor the immune status of the gingival tissues during pregnancy.","PeriodicalId":19450,"journal":{"name":"Odovtos - International Journal of Dental Sciences","volume":"13 1","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84519448","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-02-06DOI: 10.15517/ijds.2023.54077
José William Cubas-Mogollón, S. Jiménez-Sánchez, E. Ruiz-Ramírez, C. Erazo-Paredes, E. Aguirre-Siancas
The aim of this experimental study was to determine the effect of photobiomodulation therapy on bone repair in a rat tibia osteotomy model at 15 and 30 days. The sample consisted of 36 male Holtzman rats that were randomized into 6 equal groups. Groups A1 and A2: osteotomy + 1 J laser energy. Groups B1 and B2: osteotomy + 3 J laser energy. Groups C1 and C2 (controls): osteotomy only. The bone repair was analyzed by histological evaluation of osteoblasts and osteocytes both at 15 days (groups A1, B1, and C1) and at 30 days (groups A2, B2, and C2). Within the results, in all groups a greater number of osteoblasts was found at 15 days vs 30 days (p<0.05), and a greater number of osteocytes in B1 and C2 vs B2 and C1, respectively (p<0.05). When evaluating the 3 groups worked up to 15 days, more osteoblasts were found in A1 and C1 vs B1 (p<0.001); and osteocytes predominated in A1 and B1 vs C1 (p<0.001). At 30 days there was a greater quantity of osteoblasts in C2 vs A2 and B2 (p<0.05) and of osteocytes in C2 vs B2 (p<0.05). It is concluded that 1 J photobiomodulation therapy improved bone repair at 15 days; however, this improvement was not observed at 30 days because there were no differences between the irradiated groups and the control.
{"title":"Effect of Energy Level of Photobiomodulation Therapy on Bone Repair in Rats","authors":"José William Cubas-Mogollón, S. Jiménez-Sánchez, E. Ruiz-Ramírez, C. Erazo-Paredes, E. Aguirre-Siancas","doi":"10.15517/ijds.2023.54077","DOIUrl":"https://doi.org/10.15517/ijds.2023.54077","url":null,"abstract":"The aim of this experimental study was to determine the effect of photobiomodulation therapy on bone repair in a rat tibia osteotomy model at 15 and 30 days. The sample consisted of 36 male Holtzman rats that were randomized into 6 equal groups. Groups A1 and A2: osteotomy + 1 J laser energy. Groups B1 and B2: osteotomy + 3 J laser energy. Groups C1 and C2 (controls): osteotomy only. The bone repair was analyzed by histological evaluation of osteoblasts and osteocytes both at 15 days (groups A1, B1, and C1) and at 30 days (groups A2, B2, and C2). Within the results, in all groups a greater number of osteoblasts was found at 15 days vs 30 days (p<0.05), and a greater number of osteocytes in B1 and C2 vs B2 and C1, respectively (p<0.05). When evaluating the 3 groups worked up to 15 days, more osteoblasts were found in A1 and C1 vs B1 (p<0.001); and osteocytes predominated in A1 and B1 vs C1 (p<0.001). At 30 days there was a greater quantity of osteoblasts in C2 vs A2 and B2 (p<0.05) and of osteocytes in C2 vs B2 (p<0.05). It is concluded that 1 J photobiomodulation therapy improved bone repair at 15 days; however, this improvement was not observed at 30 days because there were no differences between the irradiated groups and the control.","PeriodicalId":19450,"journal":{"name":"Odovtos - International Journal of Dental Sciences","volume":"37 11 1","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82813979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-02-03DOI: 10.15517/ijds.2023.54049
M. Benavides, Erick Hernández Meza, Jessie F. Reyes-Carmona
This study aimed to analyze the effect of several final irrigation protocols on tubular permeability and push-out bond strength of an epoxy resin-based cement to radicular dentin. The crowns of 110 human teeth were removed to standardize at 17mm in length. The root canals were instrumented and irrigated with 5ml of 5% NaOCl and 17% EDTA. The samples were then randomly divided into 5 groups (n=22) according to the final irrigation protocol. Subsequently, the samples were subdivided into two different groups to assess dentin permeability or the evaluation of the push-out bond strength. In Group 1, all samples displayed the formation of brown-like precipitates. Group 2 and Group 5 demonstrated cleaner dentin walls. The push-out bond test after 72 hours denoted a significant difference when comparing Group 1 vs Group 2 and Group 1 vs Group 3. After thermocycling, a significant difference is observed between Group 1 and all remaining groups. The irrigation protocol using distilled water prior to irrigation with chlorhexidine significantly reduces the formation of intracanal precipitates by avoiding interactions between irrigants. The push-out bond strength of epoxy resin-based cement is directly influenced by the precipitates formed in the root canal after irrigation and the time of thermocycling.
{"title":"Effect of Final Irrigation Protocols on Radicular Dentin Permeability and Push-Out bond Strength in Endodontically Treated Teeth","authors":"M. Benavides, Erick Hernández Meza, Jessie F. Reyes-Carmona","doi":"10.15517/ijds.2023.54049","DOIUrl":"https://doi.org/10.15517/ijds.2023.54049","url":null,"abstract":"This study aimed to analyze the effect of several final irrigation protocols on tubular permeability and push-out bond strength of an epoxy resin-based cement to radicular dentin. The crowns of 110 human teeth were removed to standardize at 17mm in length. The root canals were instrumented and irrigated with 5ml of 5% NaOCl and 17% EDTA. The samples were then randomly divided into 5 groups (n=22) according to the final irrigation protocol. Subsequently, the samples were subdivided into two different groups to assess dentin permeability or the evaluation of the push-out bond strength. In Group 1, all samples displayed the formation of brown-like precipitates. Group 2 and Group 5 demonstrated cleaner dentin walls. The push-out bond test after 72 hours denoted a significant difference when comparing Group 1 vs Group 2 and Group 1 vs Group 3. After thermocycling, a significant difference is observed between Group 1 and all remaining groups. The irrigation protocol using distilled water prior to irrigation with chlorhexidine significantly reduces the formation of intracanal precipitates by avoiding interactions between irrigants. The push-out bond strength of epoxy resin-based cement is directly influenced by the precipitates formed in the root canal after irrigation and the time of thermocycling. ","PeriodicalId":19450,"journal":{"name":"Odovtos - International Journal of Dental Sciences","volume":"40 1","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80637948","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-02-01DOI: 10.15517/ijds.2023.54004
J. C. Pinto, Mariana Mena Barreto Pivoto-João, J. Guerreiro-Tanomaru, Jessie F. Reyes-Carmona, M. Tanomaru-Filho
The aim of this study was to compare the filling capacity in curved root canal using a new continuous wave of condensation technique (Termo Pack II, Easy Dental Equipments, Brazil) or lateral compaction. The percentage of voids in the filling of mesial root canals of mandibular molars was assessed by micro-computed tomography (micro-CT). Mesial root canals (n=24) of mandibular molars with a degree of curvature between 20° and 40° were prepared using rotary system (ProDesign Logic, Easy, Brazil) up to #35, .05 taper. The root canals were filled by using the continuous wave of condensation system or lateral compaction and AH Plus sealer (n=12). Scanning at 9 µm was performed after preparation and after filling by using micro-CT SkyScan 1176. The volumetric percentage of filling material and voids (total length and in each root canal third) were calculated. Data were analyzed using ANOVA/Tukey and Student’s t tests (α=0.05). Before the filling techniques, the root canals volume after preparation was similar (p>0.05). The root canals filled by the continuous wave of condensation technique presented the lowest percentage of voids, and the greatest percentage of filling material in total length and thirds (cervical, middle and apical) (p<0.05). Both techniques were not able of completely filling the root canals. The continuous wave of condensation technique Termo Pack II promoted better root canal filling in curved root canals, when compared with lateral compaction.
{"title":"Continuous Wave of Condensation Improves the Filling of Curved Canals: a Micro-CT Study","authors":"J. C. Pinto, Mariana Mena Barreto Pivoto-João, J. Guerreiro-Tanomaru, Jessie F. Reyes-Carmona, M. Tanomaru-Filho","doi":"10.15517/ijds.2023.54004","DOIUrl":"https://doi.org/10.15517/ijds.2023.54004","url":null,"abstract":"The aim of this study was to compare the filling capacity in curved root canal using a new continuous wave of condensation technique (Termo Pack II, Easy Dental Equipments, Brazil) or lateral compaction. The percentage of voids in the filling of mesial root canals of mandibular molars was assessed by micro-computed tomography (micro-CT). Mesial root canals (n=24) of mandibular molars with a degree of curvature between 20° and 40° were prepared using rotary system (ProDesign Logic, Easy, Brazil) up to #35, .05 taper. The root canals were filled by using the continuous wave of condensation system or lateral compaction and AH Plus sealer (n=12). Scanning at 9 µm was performed after preparation and after filling by using micro-CT SkyScan 1176. The volumetric percentage of filling material and voids (total length and in each root canal third) were calculated. Data were analyzed using ANOVA/Tukey and Student’s t tests (α=0.05). Before the filling techniques, the root canals volume after preparation was similar (p>0.05). The root canals filled by the continuous wave of condensation technique presented the lowest percentage of voids, and the greatest percentage of filling material in total length and thirds (cervical, middle and apical) (p<0.05). Both techniques were not able of completely filling the root canals. The continuous wave of condensation technique Termo Pack II promoted better root canal filling in curved root canals, when compared with lateral compaction.","PeriodicalId":19450,"journal":{"name":"Odovtos - International Journal of Dental Sciences","volume":"139 1","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74012996","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-20DOI: 10.15517/ijds.2023.53811
J. C. Pinto, É. Lucas-Oliveira, T. Bonagamba, J. Guerreiro-Tanomaru, M. Tanomaru-Filho
The aim of this study was to assess the influence of micro-computed tomography (micro-CT) voxel size on evaluation of root canal preparation using rotary heat-treated nickel-titanium files. Curved mesial root canals of mandibular molars were prepared using ProDesign Logic 30/.05 (PDL) or HyFlex EDM 25/.08 (HEDM) (n=12). The specimens were scanned using micro-CT with 5μm of voxel size before and after root canal preparation. Images with sub-resolution of 10 and 20μm voxel sizes were obtained. The percentage of volume increase, debris and uninstrumented root canal surface were analyzed in the different voxel sizes. Data were compared using unpaired Student’s t-test and ANOVA statistical tests (α=0.05). No differences were observed for percentage of volume increase, debris and instrumented surface between the root canals prepared by PDL and HEDM (p>0.05). Both systems promoted higher percentage of debris in the apical third compared to the middle third (p<0.05). After instrumentation using PDL the percentage of uninstrumented surface was highr in the apical third than middle third only when analysis were performed at 5µm (p<0.05). When comparing the different voxel sizes (5,10 or 20µm), both groups showed different means for the variables, with no significant difference (p>0.05). PDL and HEDM had similar root canal preparation capacity. Micro-CT images using different voxel sizes did not influence the results of volume increase and debris evaluation. However, images at 5µm showed greater accuracy to evaluate the percentage of uninstrumented surfaces.
{"title":"Effect of Voxel Size of Micro-CT on the Assessment of Root Canal Preparation","authors":"J. C. Pinto, É. Lucas-Oliveira, T. Bonagamba, J. Guerreiro-Tanomaru, M. Tanomaru-Filho","doi":"10.15517/ijds.2023.53811","DOIUrl":"https://doi.org/10.15517/ijds.2023.53811","url":null,"abstract":"The aim of this study was to assess the influence of micro-computed tomography (micro-CT) voxel size on evaluation of root canal preparation using rotary heat-treated nickel-titanium files. Curved mesial root canals of mandibular molars were prepared using ProDesign Logic 30/.05 (PDL) or HyFlex EDM 25/.08 (HEDM) (n=12). The specimens were scanned using micro-CT with 5μm of voxel size before and after root canal preparation. Images with sub-resolution of 10 and 20μm voxel sizes were obtained. The percentage of volume increase, debris and uninstrumented root canal surface were analyzed in the different voxel sizes. Data were compared using unpaired Student’s t-test and ANOVA statistical tests (α=0.05). No differences were observed for percentage of volume increase, debris and instrumented surface between the root canals prepared by PDL and HEDM (p>0.05). Both systems promoted higher percentage of debris in the apical third compared to the middle third (p<0.05). After instrumentation using PDL the percentage of uninstrumented surface was highr in the apical third than middle third only when analysis were performed at 5µm (p<0.05). When comparing the different voxel sizes (5,10 or 20µm), both groups showed different means for the variables, with no significant difference (p>0.05). PDL and HEDM had similar root canal preparation capacity. Micro-CT images using different voxel sizes did not influence the results of volume increase and debris evaluation. However, images at 5µm showed greater accuracy to evaluate the percentage of uninstrumented surfaces.","PeriodicalId":19450,"journal":{"name":"Odovtos - International Journal of Dental Sciences","volume":"25 1","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78032355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-16DOI: 10.15517/ijds.2023.53752
Marwa Elshafei, I. Helmy, M. M. Sayed, Doaa B Farag, Ismail Shebl, S. Ghazy, N. Afifi
Cells undergo autophagy to save themselves from injury, but progressive autophagy can cause cell death. This study characterized and compared the effect of grape (resveratrol) and tomato (lycopene) extracts and their combination on modulating autophagy-related miRNA and its target gene in squamous cell carcinoma cell line. Docking analysis for extracts and selected genes was performed. Methyl Thiazol Tetrazolium assays were used to assess the cytotoxicity of extracts and their combination toward HEp-2 cells. qRT-PCR was used to quantify changes in gene expression. Data were statistically analyzed. miRNA-20a was identified as a potential effector in laryngeal cancer, and sequestosome-1 (SQSTM1) was its target gene. Docking analysis showed that resveratrol interacted with miRNA-20a and showed less affinity toward SQSTM1. Hydrogen bonds and hydrophobic interactions were predicted. In contrast, lycopene showed less affinity toward miRNA-20a than resveratrol. Increasing doses of resveratrol, lycopene, and their combination induced a statistically significant reduction in mean percent viability and mean fold changes of miRNA-20a and SQSTM1 expression in treated HEp-2 cells. Pearson’s correlation showed a statistically significant positive correlation between miRNA-20a and SQSTM1 (R=0.812, p≤0.001). Grape and tomato extracts and their combination display promising cytotoxicity against HEp-2 cells in a dose- and time-dependent fashion. Both extracts reduce the expression of miRNA-20a and SQSTM1 with subsequent inhibition autophagy and promotion of apoptosis in HEp-2 cells.
{"title":"Autophagic-Related Anticancer Effect of Grapes Extract and Tomatoes Extract: Ex-Vivo Study","authors":"Marwa Elshafei, I. Helmy, M. M. Sayed, Doaa B Farag, Ismail Shebl, S. Ghazy, N. Afifi","doi":"10.15517/ijds.2023.53752","DOIUrl":"https://doi.org/10.15517/ijds.2023.53752","url":null,"abstract":"Cells undergo autophagy to save themselves from injury, but progressive autophagy can cause cell death. This study characterized and compared the effect of grape (resveratrol) and tomato (lycopene) extracts and their combination on modulating autophagy-related miRNA and its target gene in squamous cell carcinoma cell line. Docking analysis for extracts and selected genes was performed. Methyl Thiazol Tetrazolium assays were used to assess the cytotoxicity of extracts and their combination toward HEp-2 cells. qRT-PCR was used to quantify changes in gene expression. Data were statistically analyzed. miRNA-20a was identified as a potential effector in laryngeal cancer, and sequestosome-1 (SQSTM1) was its target gene. Docking analysis showed that resveratrol interacted with miRNA-20a and showed less affinity toward SQSTM1. Hydrogen bonds and hydrophobic interactions were predicted. In contrast, lycopene showed less affinity toward miRNA-20a than resveratrol. Increasing doses of resveratrol, lycopene, and their combination induced a statistically significant reduction in mean percent viability and mean fold changes of miRNA-20a and SQSTM1 expression in treated HEp-2 cells. Pearson’s correlation showed a statistically significant positive correlation between miRNA-20a and SQSTM1 (R=0.812, p≤0.001). Grape and tomato extracts and their combination display promising cytotoxicity against HEp-2 cells in a dose- and time-dependent fashion. Both extracts reduce the expression of miRNA-20a and SQSTM1 with subsequent inhibition autophagy and promotion of apoptosis in HEp-2 cells. \u0000 ","PeriodicalId":19450,"journal":{"name":"Odovtos - International Journal of Dental Sciences","volume":"18 1","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87165901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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