Pub Date : 2024-02-23DOI: 10.15517/ijds.2024.58908
V. Méndez-González, Joselyn Martínez-López, A. Pozos-Guillen, Ana M. González-Amaro, Mariana Gutiérrez-Sánchez, D. Escobar-García
Bioactive cements based on tricalcium silicate have been introducedto the market for use in dentistry, with a variety of clinical applications. These cements are in contact with vital tissues such as dental pulp or periodontium in cases of unintentional extrusion; thus, it is important to know the genotoxicity and cytoxicity of these materials. The objective of this study was to evaluate the cytotoxicity and genotoxicity of bioactive sealers, Bio-C® Sealer and MTA Repair HP®, in human fibroblasts. Discs of bioactive sealers Bio-C® Sealer, and MTA Repair HP®, were prepared and set for 24h under sterile conditions. The discs were placed in culture medium at 2.5mg/mL inside a SRT6D roller mixer (Stuart, UK) at 60rpm for 24h. The eluates obtained were incubated for 24h with previously activated and cultured ATCC cell line fibroblasts at 80% confluence. The cytotoxicity was evaluated by Alamar Blue® and LIVE/DEAD assays, as well as the analysis of the Tunel and Mitotracker assays to evaluate genotoxicity using the confocal laser-scanning microscope. In the Alamar Blue® assay, the Bio-C® Sealer presented a cell proliferation of 87%, while the MTA Repair HP® Sealer was 72%. A statistically significant difference was found between the MTA Repair HP® Sealant and the negative control (p=<0.001). Regarding the genotoxicity tests, in the Tunel assay, both materials stain the nucleus of the fibroblast cells exposed to the eluates, while in the Mitotracker assay, the MTA Repair HP® Sealer showed greater mitochondrial function than the Bio-C® Sealer. Calcium silicate-based sealers, Bio-C® Sealer and MTA Repair HP®, are not cytotoxic and have low genotoxicity.
{"title":"Biocompatibility of Bioactive Sealers Bio-C Sealer vs MTA Repair HP in Human Fibroblasts","authors":"V. Méndez-González, Joselyn Martínez-López, A. Pozos-Guillen, Ana M. González-Amaro, Mariana Gutiérrez-Sánchez, D. Escobar-García","doi":"10.15517/ijds.2024.58908","DOIUrl":"https://doi.org/10.15517/ijds.2024.58908","url":null,"abstract":"Bioactive cements based on tricalcium silicate have been introducedto the market for use in dentistry, with a variety of clinical applications. These cements are in contact with vital tissues such as dental pulp or periodontium in cases of unintentional extrusion; thus, it is important to know the genotoxicity and cytoxicity of these materials. The objective of this study was to evaluate the cytotoxicity and genotoxicity of bioactive sealers, Bio-C® Sealer and MTA Repair HP®, in human fibroblasts. Discs of bioactive sealers Bio-C® Sealer, and MTA Repair HP®, were prepared and set for 24h under sterile conditions. The discs were placed in culture medium at 2.5mg/mL inside a SRT6D roller mixer (Stuart, UK) at 60rpm for 24h. The eluates obtained were incubated for 24h with previously activated and cultured ATCC cell line fibroblasts at 80% confluence. The cytotoxicity was evaluated by Alamar Blue® and LIVE/DEAD assays, as well as the analysis of the Tunel and Mitotracker assays to evaluate genotoxicity using the confocal laser-scanning microscope. In the Alamar Blue® assay, the Bio-C® Sealer presented a cell proliferation of 87%, while the MTA Repair HP® Sealer was 72%. A statistically significant difference was found between the MTA Repair HP® Sealant and the negative control (p=<0.001). Regarding the genotoxicity tests, in the Tunel assay, both materials stain the nucleus of the fibroblast cells exposed to the eluates, while in the Mitotracker assay, the MTA Repair HP® Sealer showed greater mitochondrial function than the Bio-C® Sealer. Calcium silicate-based sealers, Bio-C® Sealer and MTA Repair HP®, are not cytotoxic and have low genotoxicity.","PeriodicalId":19450,"journal":{"name":"Odovtos - International Journal of Dental Sciences","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2024-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140435782","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-02-22DOI: 10.15517/ijds.2024.58892
José A. Marín-Uc, V. Aguilar-Hernández, Teresa Hernández-Sotomayor, Ligia Brito Argáez, Geovanny I. Nic-Can, Martha Gabriela Chuc-Gamboa, Fernando Aguilar-Ayala, F. Aguilar-Pérez, B. Rodas-Junco
Dental stem cells (DSCs) are multipotent cells with high proliferation capacity and multilineage differentiation. Few studies have compared the cellular characteristics and adipogenic differentiation potential of DSCs derived from tissues of the same individual. The objective of this work was to evaluate the differences in growth characteristics, expression of mesenchymal stem cells (MSCs)-specific markers, and the proteomic profile in response to adipogenic differentiation, of dental pulp and periodontal ligament cells obtained from a single donor. Dental cells were isolated from the third molar of a single donor using the outgrowth method. To obtain the proliferation curve of the cells was evaluated by trypan blue analysis. After the cells were cultured in adipogenic medium, morphological changes were monitored by oil red O staining, as well as adipogenic markers PPARγ and adiponectin by RT-qPCR. Finally, a two-dimensional electrophoresis of the proteins isolated from these cells was performed to analyze the proteomic profile. The two types of DSCs share similar cellular characteristics; however, their capacity for adipogenic differentiation is different. Based on the protein profiling results, we identified five differentially expressed proteins between both types of stem cells. Our findings showed that dental pulp and periodontal ligament stem cells from a single donor have similar cellular characteristics but a different response to adipogenesis, which would explain the differences in the expression of their proteins.
牙科干细胞（DSCs）是一种多能细胞，具有高增殖能力和多线分化能力。很少有研究比较来自同一个体组织的牙干细胞的细胞特性和成脂分化潜能。这项工作的目的是评估来自同一供体的牙髓细胞和牙周韧带细胞在生长特性、间充质干细胞（MSCs）特异性标志物的表达以及对成脂肪分化反应的蛋白质组学特征方面的差异。采用外植法从单一供体的第三磨牙中分离出牙髓细胞。通过胰蓝分析评估细胞的增殖曲线。细胞在成脂培养基中培养后，通过油红 O 染色监测形态学变化，并通过 RT-qPCR 检测成脂标志物 PPARγ 和脂肪连蛋白。最后，对从这些细胞中分离出来的蛋白质进行了二维电泳，以分析蛋白质组概况。这两种 DSCs 具有相似的细胞特征，但它们的成脂分化能力却不同。根据蛋白质图谱分析结果，我们确定了两种干细胞之间有五种不同表达的蛋白质。我们的研究结果表明，来自单一供体的牙髓干细胞和牙周韧带干细胞具有相似的细胞特征，但对脂肪生成的反应却不同，这就解释了它们在蛋白质表达上的差异。
{"title":"Comparative Analysis of Dental Stem Cells from a Single Donor: Differences in Adipogenic Differentiation and Protein Profile","authors":"José A. Marín-Uc, V. Aguilar-Hernández, Teresa Hernández-Sotomayor, Ligia Brito Argáez, Geovanny I. Nic-Can, Martha Gabriela Chuc-Gamboa, Fernando Aguilar-Ayala, F. Aguilar-Pérez, B. Rodas-Junco","doi":"10.15517/ijds.2024.58892","DOIUrl":"https://doi.org/10.15517/ijds.2024.58892","url":null,"abstract":"Dental stem cells (DSCs) are multipotent cells with high proliferation capacity and multilineage differentiation. Few studies have compared the cellular characteristics and adipogenic differentiation potential of DSCs derived from tissues of the same individual. The objective of this work was to evaluate the differences in growth characteristics, expression of mesenchymal stem cells (MSCs)-specific markers, \u0000and the proteomic profile in response to adipogenic differentiation, of dental pulp and periodontal ligament cells obtained from a single donor. Dental cells were isolated from the third molar of a single donor using the outgrowth method. To obtain the proliferation curve of the cells was evaluated by trypan blue analysis. After the cells were cultured in adipogenic medium, morphological changes were monitored by oil red O staining, as well as adipogenic markers PPARγ and adiponectin by RT-qPCR. Finally, a two-dimensional electrophoresis of the proteins isolated from these cells was performed to analyze the proteomic profile. The two types of DSCs share similar cellular characteristics; however, their capacity for adipogenic differentiation is different. Based on the protein profiling results, we identified five differentially expressed proteins between both types of stem cells. Our findings showed that dental pulp and periodontal ligament stem cells from a single donor have similar cellular characteristics but a different response to adipogenesis, which would explain the differences in the expression of their proteins.","PeriodicalId":19450,"journal":{"name":"Odovtos - International Journal of Dental Sciences","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2024-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139957719","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-02-13DOI: 10.15517/ijds.2024.58783
E. Aslantaş, H. Aksel, Hatice Dogan Buzoglu
This study aimed to investigate the impact of the combination of Benzalkonium chloride (BAC) and sodium hypochloride (NaOCl) and its application after ethylenediaminetetraacetic acid (EDTA) in root canal irrigation procedures on the microhardness and mineral content of dentin. Distal roots of mandibular third molars were embedded in auto-polymerizing acrylic resin, sectioned coronally to apically resulting in fifty-four root sections. Thirty sections underwent microhardness evaluation, and twenty-four sections were randomly chosen for mineral analysis. The microhardness assessment comprised three groups: Group 1 (2.5% NaOCl), Group 2 (2.5% NaOCl with 0.084% BAC), and Group 3 (2.5% NaOCl with 0.084 BAC post-17% EDTA). Initial and post-irrigation microhardness values were measured for each group. For mineral analysis, samples were categorized into four groups: distilled water, 2.5% NaOCl, 2.5% NaOCl with 0.084% BAC, and 17% EDTA+2.5% NaOCl with 0.084% BAC. X-ray photoelectron spectroscopy immediately measured magnesium, phosphorus, and calcium mineral contents on dentin surfaces post-irrigation. Results indicated a significant reduction in root dentin microhardness for all solutions (p<0.05). However, no statistically significant difference in the percentage of reduction was observed among the groups (p>0.05). XPS analysis revealed no significant disparity in dentin surface mineral content among the groups (p>0.05). In conclusion, the addition of Benzalkonium chloride to sodium hypochloride, either alone or after EDTA, did not induce a significant alteration in dentin microhardness or mineral content. These findings contribute to a nuanced understanding of dental irrigation protocols and their effects on dentin properties during endodontic procedures.
{"title":"Effects of a Benzalkonium Chloride Surfactant-Sodium Hypochlorite Combination on Microhardness and Mineral Content of Dentin","authors":"E. Aslantaş, H. Aksel, Hatice Dogan Buzoglu","doi":"10.15517/ijds.2024.58783","DOIUrl":"https://doi.org/10.15517/ijds.2024.58783","url":null,"abstract":"This study aimed to investigate the impact of the combination of Benzalkonium chloride (BAC) and sodium hypochloride (NaOCl) and its application after ethylenediaminetetraacetic acid (EDTA) in root canal irrigation procedures on the microhardness and mineral content of dentin. Distal roots of mandibular third molars were embedded in auto-polymerizing acrylic resin, sectioned coronally to apically resulting in fifty-four root sections. Thirty sections underwent microhardness evaluation, and twenty-four sections were randomly chosen for mineral analysis. The microhardness assessment comprised three groups: Group 1 (2.5% NaOCl), Group 2 (2.5% NaOCl with 0.084% BAC), and Group 3 (2.5% NaOCl with 0.084 BAC post-17% EDTA). Initial and post-irrigation microhardness values were measured for each group. For mineral analysis, samples were categorized into four groups: distilled water, 2.5% NaOCl, 2.5% NaOCl with 0.084% BAC, and 17% EDTA+2.5% NaOCl with 0.084% BAC. X-ray photoelectron spectroscopy immediately measured magnesium, phosphorus, and calcium mineral contents on dentin surfaces post-irrigation. Results indicated a significant reduction in root dentin microhardness for all solutions (p<0.05). However, no statistically significant difference in the percentage of reduction was observed among the groups (p>0.05). XPS analysis revealed no significant disparity in dentin surface mineral content among the groups (p>0.05). In conclusion, the addition of Benzalkonium chloride to sodium hypochloride, either alone or after EDTA, did not induce a significant alteration in dentin microhardness or mineral content. These findings contribute to a nuanced understanding of dental irrigation protocols and their effects on dentin properties during endodontic procedures.","PeriodicalId":19450,"journal":{"name":"Odovtos - International Journal of Dental Sciences","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2024-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139779855","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-02-13DOI: 10.15517/ijds.2024.58783
E. Aslantaş, H. Aksel, Hatice Dogan Buzoglu
This study aimed to investigate the impact of the combination of Benzalkonium chloride (BAC) and sodium hypochloride (NaOCl) and its application after ethylenediaminetetraacetic acid (EDTA) in root canal irrigation procedures on the microhardness and mineral content of dentin. Distal roots of mandibular third molars were embedded in auto-polymerizing acrylic resin, sectioned coronally to apically resulting in fifty-four root sections. Thirty sections underwent microhardness evaluation, and twenty-four sections were randomly chosen for mineral analysis. The microhardness assessment comprised three groups: Group 1 (2.5% NaOCl), Group 2 (2.5% NaOCl with 0.084% BAC), and Group 3 (2.5% NaOCl with 0.084 BAC post-17% EDTA). Initial and post-irrigation microhardness values were measured for each group. For mineral analysis, samples were categorized into four groups: distilled water, 2.5% NaOCl, 2.5% NaOCl with 0.084% BAC, and 17% EDTA+2.5% NaOCl with 0.084% BAC. X-ray photoelectron spectroscopy immediately measured magnesium, phosphorus, and calcium mineral contents on dentin surfaces post-irrigation. Results indicated a significant reduction in root dentin microhardness for all solutions (p<0.05). However, no statistically significant difference in the percentage of reduction was observed among the groups (p>0.05). XPS analysis revealed no significant disparity in dentin surface mineral content among the groups (p>0.05). In conclusion, the addition of Benzalkonium chloride to sodium hypochloride, either alone or after EDTA, did not induce a significant alteration in dentin microhardness or mineral content. These findings contribute to a nuanced understanding of dental irrigation protocols and their effects on dentin properties during endodontic procedures.
{"title":"Effects of a Benzalkonium Chloride Surfactant-Sodium Hypochlorite Combination on Microhardness and Mineral Content of Dentin","authors":"E. Aslantaş, H. Aksel, Hatice Dogan Buzoglu","doi":"10.15517/ijds.2024.58783","DOIUrl":"https://doi.org/10.15517/ijds.2024.58783","url":null,"abstract":"This study aimed to investigate the impact of the combination of Benzalkonium chloride (BAC) and sodium hypochloride (NaOCl) and its application after ethylenediaminetetraacetic acid (EDTA) in root canal irrigation procedures on the microhardness and mineral content of dentin. Distal roots of mandibular third molars were embedded in auto-polymerizing acrylic resin, sectioned coronally to apically resulting in fifty-four root sections. Thirty sections underwent microhardness evaluation, and twenty-four sections were randomly chosen for mineral analysis. The microhardness assessment comprised three groups: Group 1 (2.5% NaOCl), Group 2 (2.5% NaOCl with 0.084% BAC), and Group 3 (2.5% NaOCl with 0.084 BAC post-17% EDTA). Initial and post-irrigation microhardness values were measured for each group. For mineral analysis, samples were categorized into four groups: distilled water, 2.5% NaOCl, 2.5% NaOCl with 0.084% BAC, and 17% EDTA+2.5% NaOCl with 0.084% BAC. X-ray photoelectron spectroscopy immediately measured magnesium, phosphorus, and calcium mineral contents on dentin surfaces post-irrigation. Results indicated a significant reduction in root dentin microhardness for all solutions (p<0.05). However, no statistically significant difference in the percentage of reduction was observed among the groups (p>0.05). XPS analysis revealed no significant disparity in dentin surface mineral content among the groups (p>0.05). In conclusion, the addition of Benzalkonium chloride to sodium hypochloride, either alone or after EDTA, did not induce a significant alteration in dentin microhardness or mineral content. These findings contribute to a nuanced understanding of dental irrigation protocols and their effects on dentin properties during endodontic procedures.","PeriodicalId":19450,"journal":{"name":"Odovtos - International Journal of Dental Sciences","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2024-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139839596","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-02-08DOI: 10.15517/ijds.2024.58715
Natalia Gutiérrez-Marín, Ingrid Miranda-Garro, Andrea López-Soto
Social networks have become an ideal space for the exchange of information and knowledge. The objetive of this study was to determine the usefulness of the implementation of Instagram as a didactic tool to promote and facilitate student learning in the theoretical and laboratory courses of Pediatric Dentistry and Orthodontics during 2022. Private Instagram accounts were created for each of the four courses. During 4 months relevant information was posted to review concepts and strengthen the teaching-learning process of the students. At the end of the courses, a survey was conducted containing the following variables: usefulness of the social network, amount of information provided, preferred place to view content (wall or stories) and whether students recommend the use of Instagram in other courses of the Department of Dentistry and Orthodontics. For the association of variables, the chi-square test was used, value of p<0.05, 95%Cl. The participation in the accounts was 222 individuals; the interaction of the students with the accounts was 87.5% with respect to the stories, 95% observed the stories completely. There was a statistically significant difference with respect to where the information was posted: stories was the preferred place for students. There were 167 students participating in the survey. All the students found very useful or useful the content of the application on the social network.100% of the respondents recommended keeping the implementation of social networks in the theoretical courses of the Department. Instagram is not only a social application but also a channel to make the teaching process more dynamic and attractive. Teachers can integrate this social platform to foster motivation and learning in students.
{"title":"Instagram as a Pedagogical Tool in Pediatric Dentistry and Ortodontic Courses","authors":"Natalia Gutiérrez-Marín, Ingrid Miranda-Garro, Andrea López-Soto","doi":"10.15517/ijds.2024.58715","DOIUrl":"https://doi.org/10.15517/ijds.2024.58715","url":null,"abstract":"Social networks have become an ideal space for the exchange of information and knowledge. The objetive of this study was to determine the usefulness of the implementation of Instagram as a didactic tool to promote and facilitate student learning in the theoretical and laboratory courses of Pediatric Dentistry and Orthodontics during 2022. Private Instagram accounts were created for each of the four courses. During 4 months relevant information was posted to review concepts and strengthen the teaching-learning process of the students. At the end of the courses, a survey was conducted containing the following variables: usefulness of the social network, amount of information provided, preferred place to view content (wall or stories) and whether students recommend the use of Instagram in other courses of the Department of Dentistry and Orthodontics. For the association of variables, the chi-square test was used, value of p<0.05, 95%Cl. The participation in the accounts was 222 individuals; the interaction of the students with the accounts was 87.5% with respect to the stories, 95% observed the stories completely. There was a statistically significant difference with respect to where the information was posted: stories was the preferred place for students. There were 167 students participating in the survey. All the students found very useful or useful the content of the application on the social network.100% of the respondents recommended keeping the implementation of social networks in the theoretical courses of the Department. Instagram is not only a social application but also a channel to make the teaching process more dynamic and attractive. Teachers can integrate this social platform to foster motivation and learning in students.","PeriodicalId":19450,"journal":{"name":"Odovtos - International Journal of Dental Sciences","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2024-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139793500","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-02-08DOI: 10.15517/ijds.2024.58715
Natalia Gutiérrez-Marín, Ingrid Miranda-Garro, Andrea López-Soto
Social networks have become an ideal space for the exchange of information and knowledge. The objetive of this study was to determine the usefulness of the implementation of Instagram as a didactic tool to promote and facilitate student learning in the theoretical and laboratory courses of Pediatric Dentistry and Orthodontics during 2022. Private Instagram accounts were created for each of the four courses. During 4 months relevant information was posted to review concepts and strengthen the teaching-learning process of the students. At the end of the courses, a survey was conducted containing the following variables: usefulness of the social network, amount of information provided, preferred place to view content (wall or stories) and whether students recommend the use of Instagram in other courses of the Department of Dentistry and Orthodontics. For the association of variables, the chi-square test was used, value of p<0.05, 95%Cl. The participation in the accounts was 222 individuals; the interaction of the students with the accounts was 87.5% with respect to the stories, 95% observed the stories completely. There was a statistically significant difference with respect to where the information was posted: stories was the preferred place for students. There were 167 students participating in the survey. All the students found very useful or useful the content of the application on the social network.100% of the respondents recommended keeping the implementation of social networks in the theoretical courses of the Department. Instagram is not only a social application but also a channel to make the teaching process more dynamic and attractive. Teachers can integrate this social platform to foster motivation and learning in students.
{"title":"Instagram as a Pedagogical Tool in Pediatric Dentistry and Ortodontic Courses","authors":"Natalia Gutiérrez-Marín, Ingrid Miranda-Garro, Andrea López-Soto","doi":"10.15517/ijds.2024.58715","DOIUrl":"https://doi.org/10.15517/ijds.2024.58715","url":null,"abstract":"Social networks have become an ideal space for the exchange of information and knowledge. The objetive of this study was to determine the usefulness of the implementation of Instagram as a didactic tool to promote and facilitate student learning in the theoretical and laboratory courses of Pediatric Dentistry and Orthodontics during 2022. Private Instagram accounts were created for each of the four courses. During 4 months relevant information was posted to review concepts and strengthen the teaching-learning process of the students. At the end of the courses, a survey was conducted containing the following variables: usefulness of the social network, amount of information provided, preferred place to view content (wall or stories) and whether students recommend the use of Instagram in other courses of the Department of Dentistry and Orthodontics. For the association of variables, the chi-square test was used, value of p<0.05, 95%Cl. The participation in the accounts was 222 individuals; the interaction of the students with the accounts was 87.5% with respect to the stories, 95% observed the stories completely. There was a statistically significant difference with respect to where the information was posted: stories was the preferred place for students. There were 167 students participating in the survey. All the students found very useful or useful the content of the application on the social network.100% of the respondents recommended keeping the implementation of social networks in the theoretical courses of the Department. Instagram is not only a social application but also a channel to make the teaching process more dynamic and attractive. Teachers can integrate this social platform to foster motivation and learning in students.","PeriodicalId":19450,"journal":{"name":"Odovtos - International Journal of Dental Sciences","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2024-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139853481","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-02-07DOI: 10.15517/ijds.2024.58707
Rodolfo Reda, M. Seracchiani, A. Zanza, Francesco Pagnoni, Valentina Bellanova, M. D'Angelo, D. Di Nardo, E. Xhajanka, Luca Testarelli
Full-arch implant-supported fixed dental prostheses, an implant-supported prosthesis, which the patient must carefully manage in home oral hygiene procedures, is the fixed alternative to a mucosal-supported or implant-anchored prosthesis. Clinically, the execution of this rehabilitation requires four implants for the lower jaw and six implants for the upper jaw. The aim of the present study is to verify the survival-rate of implants for implant-fixed prostheses in the edentulous upper jaw. This retrospective study was carried out on patients who received an upper full-arch fixed prostheses on six implants for rehabilitation. A total of 36 patients were included and evaluated from a clinical and radiographical point of view. The follow-up period in which the data were collected on the upper full-arch is between 60 and 84 months. A total of 232 implants were inserted and monitored in this period. Clinical and radiographic evaluations were carried out on all 232 implants, with constant re-evaluation. The total implant survival rate is 93.1%, a value which is similar to previous studies already published on the topic. There were few prosthetic complications, mainly the fracture of anterior prosthetic teeth. Most of these full-arch, which as antagonist had another previously made full-arch implant-supported fixed dental prostheses or overdenture on four or overdenture on two implants, achieves good results in this study at 84 months.
{"title":"Implant Survival Rate and Prosthetic Complications of Full-arch Implant-supported Fixed Prostheses In Edentulous Upper Jaws: A Cohort Study","authors":"Rodolfo Reda, M. Seracchiani, A. Zanza, Francesco Pagnoni, Valentina Bellanova, M. D'Angelo, D. Di Nardo, E. Xhajanka, Luca Testarelli","doi":"10.15517/ijds.2024.58707","DOIUrl":"https://doi.org/10.15517/ijds.2024.58707","url":null,"abstract":"Full-arch implant-supported fixed dental prostheses, an implant-supported prosthesis, which the patient must carefully manage in home oral hygiene procedures, is the fixed alternative to a mucosal-supported or implant-anchored prosthesis. Clinically, the execution of this rehabilitation requires four implants for the lower jaw and six implants for the upper jaw. The aim of the present study is to verify the survival-rate of implants for implant-fixed prostheses in the edentulous upper jaw. This retrospective study was carried out on patients who received an upper full-arch fixed prostheses on six implants for rehabilitation. A total of 36 patients were included and evaluated from a clinical and radiographical point of view. The follow-up period in which the data were collected on the upper full-arch is between 60 and 84 months. A total of 232 implants were inserted and monitored in this period. Clinical and radiographic evaluations were carried out on all 232 implants, with constant re-evaluation. The total implant survival rate is 93.1%, a value which is similar to previous studies already published on the topic. There were few prosthetic complications, mainly the fracture of anterior prosthetic teeth. Most of these full-arch, which as antagonist had another previously made full-arch implant-supported fixed dental prostheses or overdenture on four or overdenture on two implants, achieves good results in this study at 84 months.","PeriodicalId":19450,"journal":{"name":"Odovtos - International Journal of Dental Sciences","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2024-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139796379","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-02-07DOI: 10.15517/ijds.2024.58706
R. Martínez-Martínez, J. L. Áyala-Herrera, Alejandro Nava Carmona, Rubén Abraham Domínguez Pérez, Berenice Hernández Castro, L. F. Espinosa-Cristóbal, Carlos Abud Mendoza
Th17/Treg cells and P. gingivalis have been implicated in rheumatoid arthritis (RA) and periodontitis (PE). The aim was to determine and compare Th17/Treg cells and the influence of P. gingivalis on this cell population in healthy subjects (HS) and patients with PE, RA, and PE/RA. The number of copies of P. gingivalis was performed by real-time PCR and flow cytometry was used to analyze Th17/Treg cells. In this study, PE in patients with RA increased the number of DNA copies of P. gingivalis, increasing the percentages of Th17 cells, as well as the number and regulation of Treg cells. Periodontitis influences the immune system, increasing the percentage of pro-inflammatory cells in patients with rheumatoid arthritis, which may could influence the inflammatory condition of this population.
Th17/Treg细胞和牙龈脓疱病与类风湿性关节炎（RA）和牙周炎（PE）有关。该研究旨在确定并比较健康受试者（HS）和 PE、RA 和 PE/RA 患者的 Th17/Treg 细胞以及牙龈脓疱疮对该细胞群的影响。通过实时 PCR 检测牙龈脓毒性杆菌的拷贝数，并使用流式细胞术分析 Th17/Treg 细胞。在这项研究中，RA 患者的 PE 增加了牙龈脓疱病菌 DNA 的拷贝数，提高了 Th17 细胞的百分比，同时也增加了 Treg 细胞的数量和调节能力。牙周炎会影响类风湿性关节炎患者的免疫系统，增加促炎症细胞的比例，这可能会影响该人群的炎症状况。
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Pub Date : 2024-02-07DOI: 10.15517/ijds.2024.58706
R. Martínez-Martínez, J. L. Áyala-Herrera, Alejandro Nava Carmona, Rubén Abraham Domínguez Pérez, Berenice Hernández Castro, L. F. Espinosa-Cristóbal, Carlos Abud Mendoza
Th17/Treg cells and P. gingivalis have been implicated in rheumatoid arthritis (RA) and periodontitis (PE). The aim was to determine and compare Th17/Treg cells and the influence of P. gingivalis on this cell population in healthy subjects (HS) and patients with PE, RA, and PE/RA. The number of copies of P. gingivalis was performed by real-time PCR and flow cytometry was used to analyze Th17/Treg cells. In this study, PE in patients with RA increased the number of DNA copies of P. gingivalis, increasing the percentages of Th17 cells, as well as the number and regulation of Treg cells. Periodontitis influences the immune system, increasing the percentage of pro-inflammatory cells in patients with rheumatoid arthritis, which may could influence the inflammatory condition of this population.
Th17/Treg细胞和牙龈脓疱病与类风湿性关节炎（RA）和牙周炎（PE）有关。该研究旨在确定并比较健康受试者（HS）和 PE、RA 和 PE/RA 患者的 Th17/Treg 细胞以及牙龈脓疱疮对该细胞群的影响。通过实时 PCR 检测牙龈脓毒性杆菌的拷贝数，并使用流式细胞术分析 Th17/Treg 细胞。在这项研究中，RA 患者的 PE 增加了牙龈脓疱病菌 DNA 的拷贝数，提高了 Th17 细胞的百分比，同时也增加了 Treg 细胞的数量和调节能力。牙周炎会影响类风湿性关节炎患者的免疫系统，增加促炎症细胞的比例，这可能会影响该人群的炎症状况。
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Pub Date : 2024-02-07DOI: 10.15517/ijds.2024.58707
Rodolfo Reda, M. Seracchiani, A. Zanza, Francesco Pagnoni, Valentina Bellanova, M. D'Angelo, D. Di Nardo, E. Xhajanka, Luca Testarelli
Full-arch implant-supported fixed dental prostheses, an implant-supported prosthesis, which the patient must carefully manage in home oral hygiene procedures, is the fixed alternative to a mucosal-supported or implant-anchored prosthesis. Clinically, the execution of this rehabilitation requires four implants for the lower jaw and six implants for the upper jaw. The aim of the present study is to verify the survival-rate of implants for implant-fixed prostheses in the edentulous upper jaw. This retrospective study was carried out on patients who received an upper full-arch fixed prostheses on six implants for rehabilitation. A total of 36 patients were included and evaluated from a clinical and radiographical point of view. The follow-up period in which the data were collected on the upper full-arch is between 60 and 84 months. A total of 232 implants were inserted and monitored in this period. Clinical and radiographic evaluations were carried out on all 232 implants, with constant re-evaluation. The total implant survival rate is 93.1%, a value which is similar to previous studies already published on the topic. There were few prosthetic complications, mainly the fracture of anterior prosthetic teeth. Most of these full-arch, which as antagonist had another previously made full-arch implant-supported fixed dental prostheses or overdenture on four or overdenture on two implants, achieves good results in this study at 84 months.
{"title":"Implant Survival Rate and Prosthetic Complications of Full-arch Implant-supported Fixed Prostheses In Edentulous Upper Jaws: A Cohort Study","authors":"Rodolfo Reda, M. Seracchiani, A. Zanza, Francesco Pagnoni, Valentina Bellanova, M. D'Angelo, D. Di Nardo, E. Xhajanka, Luca Testarelli","doi":"10.15517/ijds.2024.58707","DOIUrl":"https://doi.org/10.15517/ijds.2024.58707","url":null,"abstract":"Full-arch implant-supported fixed dental prostheses, an implant-supported prosthesis, which the patient must carefully manage in home oral hygiene procedures, is the fixed alternative to a mucosal-supported or implant-anchored prosthesis. Clinically, the execution of this rehabilitation requires four implants for the lower jaw and six implants for the upper jaw. The aim of the present study is to verify the survival-rate of implants for implant-fixed prostheses in the edentulous upper jaw. This retrospective study was carried out on patients who received an upper full-arch fixed prostheses on six implants for rehabilitation. A total of 36 patients were included and evaluated from a clinical and radiographical point of view. The follow-up period in which the data were collected on the upper full-arch is between 60 and 84 months. A total of 232 implants were inserted and monitored in this period. Clinical and radiographic evaluations were carried out on all 232 implants, with constant re-evaluation. The total implant survival rate is 93.1%, a value which is similar to previous studies already published on the topic. There were few prosthetic complications, mainly the fracture of anterior prosthetic teeth. Most of these full-arch, which as antagonist had another previously made full-arch implant-supported fixed dental prostheses or overdenture on four or overdenture on two implants, achieves good results in this study at 84 months.","PeriodicalId":19450,"journal":{"name":"Odovtos - International Journal of Dental Sciences","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2024-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139856362","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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