Aigul Usubalieva, Mukarama Musulmanova, A. Saalieva, Z. Ozbekova, Anara ARALBEK kyzy, Anarseit Deidiev
The paper presents a comparative characteristic of the fermentation processes of yak and cow milk samples with a high fat content of 1.5% and 6% with a multicomponent starter culture, which includes Lactobacillus acidophilus, Bifidobacterium animalis subsp Lactis and Streptococcus thermophiles. Acid formation in the process of milk fermentation under the influence of the starter microflora was assessed by the dynamics of changing in titratable (Ac) and active (pH) acidity over time. The course of the formation of the structure of the resulting clot was monitored on a rheometer, fixing the viscosity characteristics of the fermented milk clot in dynamics. It has been established that the increase in acidity occurs more intensively in yak milk in comparison with cow's milk with a corresponding acceleration of the formation of a fermented milk clot. In conclusion, the resulting clots were subjected to sensory analysis with the identification of the best sample, which was fermented yak milk with a fat mass fraction of 6%.
{"title":"The process of yak milk fermentation by polycomponent starter culture","authors":"Aigul Usubalieva, Mukarama Musulmanova, A. Saalieva, Z. Ozbekova, Anara ARALBEK kyzy, Anarseit Deidiev","doi":"10.51227/ojafr.2023.59","DOIUrl":"https://doi.org/10.51227/ojafr.2023.59","url":null,"abstract":"The paper presents a comparative characteristic of the fermentation processes of yak and cow milk samples with a high fat content of 1.5% and 6% with a multicomponent starter culture, which includes Lactobacillus acidophilus, Bifidobacterium animalis subsp Lactis and Streptococcus thermophiles. Acid formation in the process of milk fermentation under the influence of the starter microflora was assessed by the dynamics of changing in titratable (Ac) and active (pH) acidity over time. The course of the formation of the structure of the resulting clot was monitored on a rheometer, fixing the viscosity characteristics of the fermented milk clot in dynamics. It has been established that the increase in acidity occurs more intensively in yak milk in comparison with cow's milk with a corresponding acceleration of the formation of a fermented milk clot. In conclusion, the resulting clots were subjected to sensory analysis with the identification of the best sample, which was fermented yak milk with a fat mass fraction of 6%.","PeriodicalId":19485,"journal":{"name":"Online Journal of Animal and Feed Research","volume":"55 11","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139238164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Alla Kitaeva, Vira Mamedova, O. Bezaltychna, Ihor Slyusarenko, Alyona Novichkova
In the present study, the influence of levels of feeding on the formation and development of economic and commercial traits of Tsigai breed was studied in the conditions of the southern steppe of Ukraine. The research was conducted on purebred sheep from birth to 14 months of age. For this purpose, 2 groups of 3.5-4 years old ewes of the first class were selected with 40 heads in each class. It was established that poor feeding of ewes (experimental diet and below standard nutritional levels) and their offspring at the early stage of ontogenesis had a negative effect on the formation and growth of productive qualities of lambs, means of live weight and wool productivity indicators. Qualitative and quantitative indicators of wool was better in ewes obtained from mothers of the control group (received a balanced diet in accordance with the standard of feeding). Advantage in length of wool in 12 months age was 29.3%, shearing of unwashed wool (26.7%), washed (26.5%), strength of wool at 4 months of age (10.5%), and in the 14th month aged was 5%. The improvement in housing and nutrition conditions in the control group proved that the counts were better and this had a very positive effect on the productivity of the sheep. In conclusion, full-fledged feeding of ewes of the Tsigai breed ensured good development of offspring at all stages of ontogenesis and contributed to the birth of healthy, viable lambs that are capable of high productivity. Any declines in nutrients of Tsigai sheep breed (from standards of commercial formula) can cause considerable deficiency in productivity of animal.
{"title":"Productivity of the Tsigai sheep breed under different feeding regimens","authors":"Alla Kitaeva, Vira Mamedova, O. Bezaltychna, Ihor Slyusarenko, Alyona Novichkova","doi":"10.51227/ojafr.2023.62","DOIUrl":"https://doi.org/10.51227/ojafr.2023.62","url":null,"abstract":"In the present study, the influence of levels of feeding on the formation and development of economic and commercial traits of Tsigai breed was studied in the conditions of the southern steppe of Ukraine. The research was conducted on purebred sheep from birth to 14 months of age. For this purpose, 2 groups of 3.5-4 years old ewes of the first class were selected with 40 heads in each class. It was established that poor feeding of ewes (experimental diet and below standard nutritional levels) and their offspring at the early stage of ontogenesis had a negative effect on the formation and growth of productive qualities of lambs, means of live weight and wool productivity indicators. Qualitative and quantitative indicators of wool was better in ewes obtained from mothers of the control group (received a balanced diet in accordance with the standard of feeding). Advantage in length of wool in 12 months age was 29.3%, shearing of unwashed wool (26.7%), washed (26.5%), strength of wool at 4 months of age (10.5%), and in the 14th month aged was 5%. The improvement in housing and nutrition conditions in the control group proved that the counts were better and this had a very positive effect on the productivity of the sheep. In conclusion, full-fledged feeding of ewes of the Tsigai breed ensured good development of offspring at all stages of ontogenesis and contributed to the birth of healthy, viable lambs that are capable of high productivity. Any declines in nutrients of Tsigai sheep breed (from standards of commercial formula) can cause considerable deficiency in productivity of animal.","PeriodicalId":19485,"journal":{"name":"Online Journal of Animal and Feed Research","volume":"709 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139236546","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Indha Fitria Pangesti, Agus Susilo, Khothibul Umam AL AWWALY
Apis mellifera is one species of bee that produces propolis, a resin-based product. Propolis extraction using ultrasonic assistance is being widely studied. Using water as a solvent is a challenge to capture the bioactive components of propolis. This research aimed to determine the physicochemical quality resulting from the processing of propolis extract from Central Java by ultrasonics using water as a solvent at different temperatures and times. Raw propolis is extracted by the ultrasonic-assisted extraction method at low, medium, and high temperatures. Raw propolis is extracted by the ultrasonic-assisted extraction method at low, medium, and high temperatures. The study used nine treatments with three replications. The extraction time was carried out for 10, 20, and 30 minutes. The study used nine treatments with three replications. The results of the analysis showed that propolis extraction at different temperatures and times had a very significant effect (P<0.01) on the yield, total phenolic content (TPC), and total flavonoid content (TFC), with an average of 6.7–13.3%, 1.10–2.21 mg GAE/mL, and 0.07–0.32 mg QE/mL, respectively. Propolis extraction at different temperatures and times had no significant effect on tannin content, pH, and antioxidant activity. Regarding yield, TPC, TFC, and tannin content values, it was determined that extracting at high temperatures for 30 minutes produced the best results. High temperatures and long timespans are used for the best chance of collecting bioactive components.
{"title":"Ultrasonic-assisted extraction, analysis and identification of water extract of propolis","authors":"Indha Fitria Pangesti, Agus Susilo, Khothibul Umam AL AWWALY","doi":"10.51227/ojafr.2023.60","DOIUrl":"https://doi.org/10.51227/ojafr.2023.60","url":null,"abstract":"Apis mellifera is one species of bee that produces propolis, a resin-based product. Propolis extraction using ultrasonic assistance is being widely studied. Using water as a solvent is a challenge to capture the bioactive components of propolis. This research aimed to determine the physicochemical quality resulting from the processing of propolis extract from Central Java by ultrasonics using water as a solvent at different temperatures and times. Raw propolis is extracted by the ultrasonic-assisted extraction method at low, medium, and high temperatures. Raw propolis is extracted by the ultrasonic-assisted extraction method at low, medium, and high temperatures. The study used nine treatments with three replications. The extraction time was carried out for 10, 20, and 30 minutes. The study used nine treatments with three replications. The results of the analysis showed that propolis extraction at different temperatures and times had a very significant effect (P<0.01) on the yield, total phenolic content (TPC), and total flavonoid content (TFC), with an average of 6.7–13.3%, 1.10–2.21 mg GAE/mL, and 0.07–0.32 mg QE/mL, respectively. Propolis extraction at different temperatures and times had no significant effect on tannin content, pH, and antioxidant activity. Regarding yield, TPC, TFC, and tannin content values, it was determined that extracting at high temperatures for 30 minutes produced the best results. High temperatures and long timespans are used for the best chance of collecting bioactive components.","PeriodicalId":19485,"journal":{"name":"Online Journal of Animal and Feed Research","volume":"19 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139236452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Nur Ducha, Lisa Lisdiana, Guntur Trimulyono, Fitriari Izzatunnisa Muhaimin, Nur Nadiah Md Yusof, Nurdiana Samsulrizal, Razif Dasiman, Ahmad Fudhaili, Giyanita Rahma Ayu Pramesti
Senduro goats, a local breed of meat and dairy goats from Indonesia, are recognized for their significance in improving goat breeding and preserving valuable genetic resources. However, limited information exists regarding the reproductive physiology of Senduro goats, which poses challenges to the development of breeding programs and the preservation of genetic resources. This study aimed to investigate the morphological and reproductive profiles of male Senduro goats at different ages, focusing on morphological characteristics, mating behavior, and sperm quality. Morphological characteristics are assessed through body length measurements, while mating behavior serves as an indicator of reproductive behavior. Macroscopic evaluations of sperm quality include assessments of color, viscosity, pH, and volume, while microscopic examinations encompass motility (mass and individual), viability, and spermatozoa membrane integrity. The results showed morphological similarities between juvenile and adult samples, with their testicular size being the only significant difference. Based on macroscopic and microscopic examinations, no significant differences were found between groups. From the results it was concluded that there were no distinct differences in morphological characteristics, mating behavior, and sperm quality between male Senduro goats in the juvenile and adult stages.
{"title":"Morphology and reproductivity profiling of male Senduro goats based on age differences","authors":"Nur Ducha, Lisa Lisdiana, Guntur Trimulyono, Fitriari Izzatunnisa Muhaimin, Nur Nadiah Md Yusof, Nurdiana Samsulrizal, Razif Dasiman, Ahmad Fudhaili, Giyanita Rahma Ayu Pramesti","doi":"10.51227/ojafr.2023.63","DOIUrl":"https://doi.org/10.51227/ojafr.2023.63","url":null,"abstract":"Senduro goats, a local breed of meat and dairy goats from Indonesia, are recognized for their significance in improving goat breeding and preserving valuable genetic resources. However, limited information exists regarding the reproductive physiology of Senduro goats, which poses challenges to the development of breeding programs and the preservation of genetic resources. This study aimed to investigate the morphological and reproductive profiles of male Senduro goats at different ages, focusing on morphological characteristics, mating behavior, and sperm quality. Morphological characteristics are assessed through body length measurements, while mating behavior serves as an indicator of reproductive behavior. Macroscopic evaluations of sperm quality include assessments of color, viscosity, pH, and volume, while microscopic examinations encompass motility (mass and individual), viability, and spermatozoa membrane integrity. The results showed morphological similarities between juvenile and adult samples, with their testicular size being the only significant difference. Based on macroscopic and microscopic examinations, no significant differences were found between groups. From the results it was concluded that there were no distinct differences in morphological characteristics, mating behavior, and sperm quality between male Senduro goats in the juvenile and adult stages.","PeriodicalId":19485,"journal":{"name":"Online Journal of Animal and Feed Research","volume":"11 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139237503","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
R. Al-Atiyat, Mustafa AL-RAWASHDEH, Khaled Abu-Alruz, Muawya Alasasfa, Naser Salameh, Firas AL-NAWAISAH, Sami AL-KHAMAISEH, M. Tabbaa
Indigenous chickens of Jordan are facing extinction and need genetic conservation because they were unable to commercially compete in poultry industry because of low genetic ability compared to commercial layers. The study aimed to investigate phenotypic and genetic diversity of village chickens in the Jordan using discriminant analyses procedures to provide a basis for sustainable genetic conservation and utilization program to overcome any possible extinction. The sampled chicken population of 578 one-year old chickens (125 males and 433 females) was phenotypically characterized for 15 biometric and plumage traits from major cities of the three regions; Middle, North, and South. The traits variations within and between breeds were detected statistically by stepwise discriminant and canonical-discriminant of uni- and multivariate analyses. The results showed the sampled population as village chickens in Jordan is comprised of indigenous (Baladi) breed (85%) and few exotic and commercial breeds. The breeds were distinct and differentiated based on phenotypic traits indicating high genetic variability. The major phenotypic traits that showed significant power to differentiate breeds were comb type, body weight, comb size, earlobe color, wattle size, face color and breast size in males and comb type and size, body weight, face and breast size, leg color and wattle size in females. Recent and past crossings, admixture or migration from exotic and commercial breeds were noted. Moreover, low levels of phylogeographic structure were observed across the studied breeds. In conclusion, there is need to conserve the indigenous breed in situ and in vivo for its adaptive gene pool in coming days of persisted climate change and diseases threats.
{"title":"Phenotypic characterization and genetic diversity of indigenous chickens of Jordan in comparison with native and commercial breeds for conservation and breeding purposes","authors":"R. Al-Atiyat, Mustafa AL-RAWASHDEH, Khaled Abu-Alruz, Muawya Alasasfa, Naser Salameh, Firas AL-NAWAISAH, Sami AL-KHAMAISEH, M. Tabbaa","doi":"10.51227/ojafr.2023.58","DOIUrl":"https://doi.org/10.51227/ojafr.2023.58","url":null,"abstract":"Indigenous chickens of Jordan are facing extinction and need genetic conservation because they were unable to commercially compete in poultry industry because of low genetic ability compared to commercial layers. The study aimed to investigate phenotypic and genetic diversity of village chickens in the Jordan using discriminant analyses procedures to provide a basis for sustainable genetic conservation and utilization program to overcome any possible extinction. The sampled chicken population of 578 one-year old chickens (125 males and 433 females) was phenotypically characterized for 15 biometric and plumage traits from major cities of the three regions; Middle, North, and South. The traits variations within and between breeds were detected statistically by stepwise discriminant and canonical-discriminant of uni- and multivariate analyses. The results showed the sampled population as village chickens in Jordan is comprised of indigenous (Baladi) breed (85%) and few exotic and commercial breeds. The breeds were distinct and differentiated based on phenotypic traits indicating high genetic variability. The major phenotypic traits that showed significant power to differentiate breeds were comb type, body weight, comb size, earlobe color, wattle size, face color and breast size in males and comb type and size, body weight, face and breast size, leg color and wattle size in females. Recent and past crossings, admixture or migration from exotic and commercial breeds were noted. Moreover, low levels of phylogeographic structure were observed across the studied breeds. In conclusion, there is need to conserve the indigenous breed in situ and in vivo for its adaptive gene pool in coming days of persisted climate change and diseases threats.","PeriodicalId":19485,"journal":{"name":"Online Journal of Animal and Feed Research","volume":"43 30","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139237594","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This study aimed to determine the effect of adding sonicated coconut shell liquid smoke to pekin duck jerky with different storage times at room temperature and vacuum packed. Ground duck jerky is made from Pekin duck meat (Anas platyrhynchos domesticus) soaked in coconut shell liquid smoke (CSLS) which has been sonicated for 20 minutes and seasoned with spices such as garlic, galangal, coriander, tamarind, salt, and coconut sugar. A laboratory experiment was done using a completely randomized design (CRD) consisting of 5 treatments (control: 0 day storage period, T1: 7 days, T2: 14 days, T3: 21 days, and T4: 28 days) and 4 replications. The results showed that the addition of sonicated CSLS with differences in the shelf life of pekin ground duck jerky had a significant effect (P<0.01) on pH, texture, color L, a*, b*, Aw, water content, fat, carbohydrates by difference, thiobarbituric acid (TBA), and iodine number. Had a significant effect (P<0.05) on ash content, and had no significant effect on Water Holding Capacity (WHC), protein content, and organoleptic quality. It was concluded that storing ground duck jerky for 14 days at room temperature and vacuum packed did not show any damage to pH, water activity, water content, fat, protein, TBA and iodine number, and did not occur rancidity.
{"title":"Physico-chemical and sensory quality of Pekin duck jerky sonicated with coconut shell liquid smoke and stored for different periods","authors":"Nitya SALSABILA, Djalal ROSYIDI, Agus SUSILO","doi":"10.51227/ojafr.2023.53","DOIUrl":"https://doi.org/10.51227/ojafr.2023.53","url":null,"abstract":"This study aimed to determine the effect of adding sonicated coconut shell liquid smoke to pekin duck jerky with different storage times at room temperature and vacuum packed. Ground duck jerky is made from Pekin duck meat (Anas platyrhynchos domesticus) soaked in coconut shell liquid smoke (CSLS) which has been sonicated for 20 minutes and seasoned with spices such as garlic, galangal, coriander, tamarind, salt, and coconut sugar. A laboratory experiment was done using a completely randomized design (CRD) consisting of 5 treatments (control: 0 day storage period, T1: 7 days, T2: 14 days, T3: 21 days, and T4: 28 days) and 4 replications. The results showed that the addition of sonicated CSLS with differences in the shelf life of pekin ground duck jerky had a significant effect (P<0.01) on pH, texture, color L, a*, b*, Aw, water content, fat, carbohydrates by difference, thiobarbituric acid (TBA), and iodine number. Had a significant effect (P<0.05) on ash content, and had no significant effect on Water Holding Capacity (WHC), protein content, and organoleptic quality. It was concluded that storing ground duck jerky for 14 days at room temperature and vacuum packed did not show any damage to pH, water activity, water content, fat, protein, TBA and iodine number, and did not occur rancidity.","PeriodicalId":19485,"journal":{"name":"Online Journal of Animal and Feed Research","volume":"74 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135084095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Maggot’s frass is waste from cultivating maggots (insect larvae) which consists of media from maggot cultivation mixed with feces, skin and dead body of the maggots. The aim of the study was to examine the organoleptic quality, chemistry, worm eggs, lead (Pb) as heavy metal and microbiological profile of maggot’s frass as an alternative ingredient of poultry feed. A completely randomized design (CRD) with 3 treatments (T1: frass media for household waste, T2: frass media for tofu dregs, and T3: frass media for vegetable and fruit waste) and 7 replications was used. The results showed that there was no effect of different types of media treatment on the organoleptic quality, chemistry and microbiological profile of maggot’s frass. The results of chemical analysis of maggot’s frass revealed moisture of 26.39 - 46.26%, crude protein of 10.92 - 16.37%, worm eggs in the dregs media tofu (16 EPG), vegetable and fruit waste (32 EPG), total bacteria of 1.91-4.95 x 108cfu/g, and no any Escherichia coli and Salmonella isolates. Maggot’s frass which comes from fruit and vegetable waste was recommended. Therefore, maggot feed using fruit and vegetable waste treatment is recommended because of its high crude protein and metabolic energy and also without any E.coli and Salmonella contamination.
{"title":"The organoleptic, chemical and microbiological quality of maggot s frass as alternative poultry feed ingredients","authors":"Cahya Setya UTAMA, Bambang SULISTIYANTO, Binti MARIFAH, Rona Indra CAHYA","doi":"10.51227/ojafr.2023.49","DOIUrl":"https://doi.org/10.51227/ojafr.2023.49","url":null,"abstract":"Maggot’s frass is waste from cultivating maggots (insect larvae) which consists of media from maggot cultivation mixed with feces, skin and dead body of the maggots. The aim of the study was to examine the organoleptic quality, chemistry, worm eggs, lead (Pb) as heavy metal and microbiological profile of maggot’s frass as an alternative ingredient of poultry feed. A completely randomized design (CRD) with 3 treatments (T1: frass media for household waste, T2: frass media for tofu dregs, and T3: frass media for vegetable and fruit waste) and 7 replications was used. The results showed that there was no effect of different types of media treatment on the organoleptic quality, chemistry and microbiological profile of maggot’s frass. The results of chemical analysis of maggot’s frass revealed moisture of 26.39 - 46.26%, crude protein of 10.92 - 16.37%, worm eggs in the dregs media tofu (16 EPG), vegetable and fruit waste (32 EPG), total bacteria of 1.91-4.95 x 108cfu/g, and no any Escherichia coli and Salmonella isolates. Maggot’s frass which comes from fruit and vegetable waste was recommended. Therefore, maggot feed using fruit and vegetable waste treatment is recommended because of its high crude protein and metabolic energy and also without any E.coli and Salmonella contamination.","PeriodicalId":19485,"journal":{"name":"Online Journal of Animal and Feed Research","volume":"18 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135084228","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Microplastics (MPs) the products of plastic breakdown, are entering the environment as a result of plastic abuse, which are of size less than 5mm. Due to their ubiquitous nature, MPs have become a significant environmental concern. One alarming area of MPs contamination is their potential presence in the feed of edible animal species. Growing research suggests that MPs can enter food products and subsequently move to various trophic levels of food chains. Hence, assessing the threat of MPs contamination in animal feed is important for food security and human health. In this investigation, 36 livestock and poultry feed samples were collected from 12 different farms, MPs were detected using Fourier Transform Infrared Spectroscopy (FTIR) and Differential Scanning Calorimeter (DSC). The Nano particle analyser was used to determine the size distribution, and Pyrolysis-GC/MS was used to quantify MPs. According to the findings, all the feed samples contained a significant amount of Polyethylene terephthalate (PET), Polypropylene (PP), and Polyvinyl chloride (PVC) and the particle size ranged from 2.02 to 10.7 µm. Present study has given detailed information on the size distribution of MPs in animal feed, which is thought to enable them to pass through membrane barriers. From the findings it is evident that there are high chances of MPs entering animal feed due to the continuous contact of the feed with plastic-based materials. These MPs can accumulate in the tissues of animals and potentially be transferred to humans through the consumption of meat, milk, and other animal-derived products. Subsequently these MPs can finally bio-accumulate in humans and cause serious health issues.
{"title":"Detection and characterisation of microplastics in animal feed","authors":"Sharon Sushma MAGANTI, Rajani Chowdary AKKINA","doi":"10.51227/ojafr.2023.50","DOIUrl":"https://doi.org/10.51227/ojafr.2023.50","url":null,"abstract":"Microplastics (MPs) the products of plastic breakdown, are entering the environment as a result of plastic abuse, which are of size less than 5mm. Due to their ubiquitous nature, MPs have become a significant environmental concern. One alarming area of MPs contamination is their potential presence in the feed of edible animal species. Growing research suggests that MPs can enter food products and subsequently move to various trophic levels of food chains. Hence, assessing the threat of MPs contamination in animal feed is important for food security and human health. In this investigation, 36 livestock and poultry feed samples were collected from 12 different farms, MPs were detected using Fourier Transform Infrared Spectroscopy (FTIR) and Differential Scanning Calorimeter (DSC). The Nano particle analyser was used to determine the size distribution, and Pyrolysis-GC/MS was used to quantify MPs. According to the findings, all the feed samples contained a significant amount of Polyethylene terephthalate (PET), Polypropylene (PP), and Polyvinyl chloride (PVC) and the particle size ranged from 2.02 to 10.7 µm. Present study has given detailed information on the size distribution of MPs in animal feed, which is thought to enable them to pass through membrane barriers. From the findings it is evident that there are high chances of MPs entering animal feed due to the continuous contact of the feed with plastic-based materials. These MPs can accumulate in the tissues of animals and potentially be transferred to humans through the consumption of meat, milk, and other animal-derived products. Subsequently these MPs can finally bio-accumulate in humans and cause serious health issues.","PeriodicalId":19485,"journal":{"name":"Online Journal of Animal and Feed Research","volume":"66 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135084435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yousra M. NASSAR, Wafaa A. ABD EL-GHANY, Adel K. IBRAHIM, Ahmed S. HAMOUDA
Paratyphoid salmonellosis is a serious disease threatens the poultry industry worldwide, besides its public health hazard. The aims of this study were characterization of paratyphoid Salmonella spp. in chicken flocks of some Egyptian governorates, demonstration of the antimicrobial susceptibility of the isolated Salmonella spp., and detection of some virulence genes and antibiotic resistance genes using recent molecular techniques. A total of 238 organ samples were collected from 52 broiler, layer, and breeder chicken flocks, representing 9 Egyptian governorates. Conventional characterization of Salmonella isolates revealed a total isolation rate of 56.3% (134/238). Moreover, the isolation rates of Salmonella spp. were (49/79; 62%), (47/81; 58%), (10/18; 55.5%), (9/20; 45%), (2/6; 33.3%), (2/3; 66.7%), and (15/82; 53.6%) from liver, yolk sac, heart, spleen, caecum, ovary, and dead-in-shell embryos, respectively. A total of 32/238 (13.44%) isolates of Salmonella were found. Serological identification revealed presence of S. enteritidis (21.9%), S. kentucky (15.6%), S. typhimurium (12.5%), S. molade (12.5%), S. takoradi (9.4%), S. wingrove (6.3%), S. infantis (6.3%), S. tsevie (6.3), S. shangani (3.1%), S. bargny (3.1%), and S. papuana (3.1%). All Salmonella strains (32/32; 100%) were resistant to streptomycin, while almost all of them (31/32; 96.9%) were susceptible to meropenem. The amplification of 16S rRNA gene of Salmonella isolates using uniplex polymerase chain reaction (PCR) generated a specific Salmonella product of approximate 550 base pair. The multiplex PCR revealed presence of invA (100%), stn (65.6%), and sopB (40.6 %) virulence-associated genes as well as aadA1 (100%), blaTEM (59.4%), aadB (18.75%), and sul1 (28.1%) antibiotic resistance genes. In conclusion, virulent paratyphoid Salmonella spp. are circulating in the Egyptian flocks, causing economic loses. Additionally, they became resistant to the most commonly used field antibiotics. Therefore, regular molecular surveillance studies on the circulating Salmonella spp. and their resistance to the used antibiotics are of significant importance.
{"title":"Paratyphoid Salmonella serovars in chickens: molecular detection of virulence and antimicrobial resistance genes","authors":"Yousra M. NASSAR, Wafaa A. ABD EL-GHANY, Adel K. IBRAHIM, Ahmed S. HAMOUDA","doi":"10.51227/ojafr.2023.56","DOIUrl":"https://doi.org/10.51227/ojafr.2023.56","url":null,"abstract":"Paratyphoid salmonellosis is a serious disease threatens the poultry industry worldwide, besides its public health hazard. The aims of this study were characterization of paratyphoid Salmonella spp. in chicken flocks of some Egyptian governorates, demonstration of the antimicrobial susceptibility of the isolated Salmonella spp., and detection of some virulence genes and antibiotic resistance genes using recent molecular techniques. A total of 238 organ samples were collected from 52 broiler, layer, and breeder chicken flocks, representing 9 Egyptian governorates. Conventional characterization of Salmonella isolates revealed a total isolation rate of 56.3% (134/238). Moreover, the isolation rates of Salmonella spp. were (49/79; 62%), (47/81; 58%), (10/18; 55.5%), (9/20; 45%), (2/6; 33.3%), (2/3; 66.7%), and (15/82; 53.6%) from liver, yolk sac, heart, spleen, caecum, ovary, and dead-in-shell embryos, respectively. A total of 32/238 (13.44%) isolates of Salmonella were found. Serological identification revealed presence of S. enteritidis (21.9%), S. kentucky (15.6%), S. typhimurium (12.5%), S. molade (12.5%), S. takoradi (9.4%), S. wingrove (6.3%), S. infantis (6.3%), S. tsevie (6.3), S. shangani (3.1%), S. bargny (3.1%), and S. papuana (3.1%). All Salmonella strains (32/32; 100%) were resistant to streptomycin, while almost all of them (31/32; 96.9%) were susceptible to meropenem. The amplification of 16S rRNA gene of Salmonella isolates using uniplex polymerase chain reaction (PCR) generated a specific Salmonella product of approximate 550 base pair. The multiplex PCR revealed presence of invA (100%), stn (65.6%), and sopB (40.6 %) virulence-associated genes as well as aadA1 (100%), blaTEM (59.4%), aadB (18.75%), and sul1 (28.1%) antibiotic resistance genes. In conclusion, virulent paratyphoid Salmonella spp. are circulating in the Egyptian flocks, causing economic loses. Additionally, they became resistant to the most commonly used field antibiotics. Therefore, regular molecular surveillance studies on the circulating Salmonella spp. and their resistance to the used antibiotics are of significant importance.","PeriodicalId":19485,"journal":{"name":"Online Journal of Animal and Feed Research","volume":"49 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135084445","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The Silkie chicken (Gallus gallus domesticus Brisson) is one of domestic chicken breeds with commercial rearing and breeding potentials for egg production. Prolactin (PRL) and dopamine D2 receptor (DRD2) are potential genes associated with reproductive traits in chickens. This study was conducted to analyze the association of PRL and DRD2 insertion/deletion (Indel) polymorphisms with chicken reproductive traits in Silkie chickens. A total of 380 hens from 16-40 weeks of age were used, with each one being placed in a separate cage. DNA isolation was performed using feather samples, and genotypes were detected using the Indel technique. Two polymorphisms consisting of 24 base pair (bp) Indel in the promoter region of the PRL gene and 22 bp Indel in the promoter region of the DRD2 gene were identified. At both sites, the Indel polymorphisms did not follow the Hardy-Weinberg equilibrium. In addition, with the exception of total eggs over 23 weeks of laying in the PRL gene, the analysis revealed no association between these polymorphic loci and any traits collected. In conclusion, birds with the DD genotype produced the maximum egg yield (73.6 eggs/hen), whereas those with the II genotype produced approximately 9 fewer eggs (64.1 eggs/hen), resulting in laying rates of 45.7% and 40.1%, respectively. For enhancing the egg-laying capacity of Silkie chickens via selective breeding, opting for DD birds with DD genotype of PRL Indel is highly recommended.
{"title":"Associations of polymorphisms in prolactin and dopamine receptor D2 genes with reproductive traits on Silkie chicken","authors":"Tran Trung TU, Le Thanh PHUONG, Nguyen Trong NGU","doi":"10.51227/ojafr.2023.47","DOIUrl":"https://doi.org/10.51227/ojafr.2023.47","url":null,"abstract":"The Silkie chicken (Gallus gallus domesticus Brisson) is one of domestic chicken breeds with commercial rearing and breeding potentials for egg production. Prolactin (PRL) and dopamine D2 receptor (DRD2) are potential genes associated with reproductive traits in chickens. This study was conducted to analyze the association of PRL and DRD2 insertion/deletion (Indel) polymorphisms with chicken reproductive traits in Silkie chickens. A total of 380 hens from 16-40 weeks of age were used, with each one being placed in a separate cage. DNA isolation was performed using feather samples, and genotypes were detected using the Indel technique. Two polymorphisms consisting of 24 base pair (bp) Indel in the promoter region of the PRL gene and 22 bp Indel in the promoter region of the DRD2 gene were identified. At both sites, the Indel polymorphisms did not follow the Hardy-Weinberg equilibrium. In addition, with the exception of total eggs over 23 weeks of laying in the PRL gene, the analysis revealed no association between these polymorphic loci and any traits collected. In conclusion, birds with the DD genotype produced the maximum egg yield (73.6 eggs/hen), whereas those with the II genotype produced approximately 9 fewer eggs (64.1 eggs/hen), resulting in laying rates of 45.7% and 40.1%, respectively. For enhancing the egg-laying capacity of Silkie chickens via selective breeding, opting for DD birds with DD genotype of PRL Indel is highly recommended.","PeriodicalId":19485,"journal":{"name":"Online Journal of Animal and Feed Research","volume":"40 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135084451","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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