Some secondary metabolites of plants could serve as ruminant feed additives. They primarily preserve protein from rumen breakdown, reduce rumen protozoa population, and decrease methane gas production. The current study aimed to identify the phytocompounds content of extracted Mucuna pruriens leaves using the Microwave-assisted extraction method using three different solvents of methanol 70% (EM), aquadest (EA), and combinations of EM and EA (EK). The phytocompounds were identified by Gas Chromatography-Mass Spectrometry (GC-MS) analysis. Some phytocompounds identified in the Mucuna pruriens substances from GC-MS curve proportion area of EM were 10.35% inositol, 3.1% quinazoline, 4.72% anthraquinone, 3.76% Coptisine, 2.06% isoquinoline, 2.18% D-gluconic acid, 2.83% D-Fructose, 3.91% D-glucose, and 4.59% butanedioic acid. The phytocompounds for EK were 17.22% inositol, 6.36% Niclosamide, 1.4% Acetamide, 1.32% Aniline, 55.97% 4-Amino-2-(4-methoxyphenyl)-5,6,7,8-tetrahydrofuro[2,3-b] quinoline-3-carbonitrile, 17.22% inositol. Furthermore, 22.73% inositol, 6.55%, ribonoic acid, 5.58%, silanol, 21.27% butanodioic acid, 2.88% Fluoroquinoxaline, 5,31%, glycerol, 1,64%, D- gluconic acid were found in the EA. The EA had high inositol content, the EK had high quinoline content, and the EM showed moderate results for all phytobiotics. The total phenolics, flavonoids, tannins, and saponins content significantly differed among the three solvents. The EA yielded the highest concentrations of total phenolics, flavonoids, and tannins, but the lowest concentration of total saponins. In contrast, the EM yielded the lowest total phenolics, flavonoids, and tannins content, but the highest total saponins content. Meanwhile, the EK yielded modest results for all phytocompounds, with values between EA and EM. In conclusion, the methanolic extract of Mucuna pruriens substance had the highest phytocompounds and bioactive potential as ruminant feed additives.
{"title":"Extracting phytocompounds from Mucuna pruriens leaves as potential ruminant feed additives using different solvents","authors":"Triana Muhartatik, S. Chuzaemi, M. Natsir","doi":"10.51227/ojafr.2023.27","DOIUrl":"https://doi.org/10.51227/ojafr.2023.27","url":null,"abstract":"Some secondary metabolites of plants could serve as ruminant feed additives. They primarily preserve protein from rumen breakdown, reduce rumen protozoa population, and decrease methane gas production. The current study aimed to identify the phytocompounds content of extracted Mucuna pruriens leaves using the Microwave-assisted extraction method using three different solvents of methanol 70% (EM), aquadest (EA), and combinations of EM and EA (EK). The phytocompounds were identified by Gas Chromatography-Mass Spectrometry (GC-MS) analysis. Some phytocompounds identified in the Mucuna pruriens substances from GC-MS curve proportion area of EM were 10.35% inositol, 3.1% quinazoline, 4.72% anthraquinone, 3.76% Coptisine, 2.06% isoquinoline, 2.18% D-gluconic acid, 2.83% D-Fructose, 3.91% D-glucose, and 4.59% butanedioic acid. The phytocompounds for EK were 17.22% inositol, 6.36% Niclosamide, 1.4% Acetamide, 1.32% Aniline, 55.97% 4-Amino-2-(4-methoxyphenyl)-5,6,7,8-tetrahydrofuro[2,3-b] quinoline-3-carbonitrile, 17.22% inositol. Furthermore, 22.73% inositol, 6.55%, ribonoic acid, 5.58%, silanol, 21.27% butanodioic acid, 2.88% Fluoroquinoxaline, 5,31%, glycerol, 1,64%, D- gluconic acid were found in the EA. The EA had high inositol content, the EK had high quinoline content, and the EM showed moderate results for all phytobiotics. The total phenolics, flavonoids, tannins, and saponins content significantly differed among the three solvents. The EA yielded the highest concentrations of total phenolics, flavonoids, and tannins, but the lowest concentration of total saponins. In contrast, the EM yielded the lowest total phenolics, flavonoids, and tannins content, but the highest total saponins content. Meanwhile, the EK yielded modest results for all phytocompounds, with values between EA and EM. In conclusion, the methanolic extract of Mucuna pruriens substance had the highest phytocompounds and bioactive potential as ruminant feed additives.","PeriodicalId":19485,"journal":{"name":"Online Journal of Animal and Feed Research","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42974703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This study aimed to obtain the best ratio of inoculums and types of alternative media in increasing the growth of the probiotic consortium with the observed variables consisting of viability, cell biomass and decrease in pH. Completely randomized design (CRD) factorial consisting of 2 factors with 3 replications, factor A were the probiotic consortium (A1: Lactobacillus parabuchneri: L. buchneri: L. harbinensis, Schieferilactobacillus harbinensis and Lentilactobacillus parabuchner) with ratio 1:1:1:1:1; A2: same consortium with ratio 1:1:1:1:2; A3: same consortium with ratio 1:1:1:2:1; A4: same consortium with ratio 1:1:2:1:1; A5: same consortium with ratio 1:2:1:1:1; A6: same consortium with ratio 2:1:1:1:1 and factor B were the type of alternative media (B1=control; B2=coconut water (90%) + cassava flour (5%) + fish waste flour (5%); B3=tofu liquid waste (90%) + flour onggok (5%) + fish waste meal (5%); B4= tofu whey (90%) + onggok flour (5%) + fish waste meal (5%). The results showed that there was an interaction between factor A and factor B which was highly significant (P<0.01) on viability, cell biomass and decrease in medium pH. In conclusion, the best ratio of probiotic consortium was 1:1:1:2:1, with growth medium coconut water (90%) + cassava flour (5%) + fish waste flour (5%) which resulted in a viability value of: 3, 02, cell biomass: 22.47 mg/ml and a decrease in the pH of the medium by 2.84.
{"title":"New growth medium for culturing lactic acid bacteria as probiotic consortium isolated from fermented fish (Budu)","authors":"Malikil Kudus Susalam, Y. Marlida","doi":"10.51227/ojafr.2023.30","DOIUrl":"https://doi.org/10.51227/ojafr.2023.30","url":null,"abstract":"This study aimed to obtain the best ratio of inoculums and types of alternative media in increasing the growth of the probiotic consortium with the observed variables consisting of viability, cell biomass and decrease in pH. Completely randomized design (CRD) factorial consisting of 2 factors with 3 replications, factor A were the probiotic consortium (A1: Lactobacillus parabuchneri: L. buchneri: L. harbinensis, Schieferilactobacillus harbinensis and Lentilactobacillus parabuchner) with ratio 1:1:1:1:1; A2: same consortium with ratio 1:1:1:1:2; A3: same consortium with ratio 1:1:1:2:1; A4: same consortium with ratio 1:1:2:1:1; A5: same consortium with ratio 1:2:1:1:1; A6: same consortium with ratio 2:1:1:1:1 and factor B were the type of alternative media (B1=control; B2=coconut water (90%) + cassava flour (5%) + fish waste flour (5%); B3=tofu liquid waste (90%) + flour onggok (5%) + fish waste meal (5%); B4= tofu whey (90%) + onggok flour (5%) + fish waste meal (5%). The results showed that there was an interaction between factor A and factor B which was highly significant (P<0.01) on viability, cell biomass and decrease in medium pH. In conclusion, the best ratio of probiotic consortium was 1:1:1:2:1, with growth medium coconut water (90%) + cassava flour (5%) + fish waste flour (5%) which resulted in a viability value of: 3, 02, cell biomass: 22.47 mg/ml and a decrease in the pH of the medium by 2.84.","PeriodicalId":19485,"journal":{"name":"Online Journal of Animal and Feed Research","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48536847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ngo Thi Kim Chi, Pham The Hue, Tran Quang Hanh, L. D. Ngoan
This study aimed to evaluate carcass characteristics and meat quality of cross-bred (Brahman × Lai Sind, BL) bulls and cross-bred (Red Angus × Lai Sind, AL) bulls. A total of 30 bulls, 15 head/crossbred genotype were fattened for 90 days before slaughtering at 24 months of age. Carcass traits and meat quality were accordingly measured in 30 slaughtered animals. Results showed that the slaughter weight, carcass weight, carcass dressing, meat percentage, loin muscle area were higher for AL bulls than for BL bulls (p<0.05). The color of the meat was not affected by genotype with exception of L* at 48, 168 and 336 hours after slaughter, and this value was higher in AL than in BL bulls (p<0.05). The pH of the meat was not different between genotypes (p>0.05) but decreased quickly at 24 hours after slaughter (p<0.05), then maintained not significantly during storage times. The drip loss, cooking loss and tenderness of the meat were affected by cattle genotype and these values were lower in AL bulls than in BL bulls (p<0.05). In conclusion, crossbred (Red Angus × Lai Sind) bulls were higher carcass characteristics, and were better meat quality than crossbred (Brahman × Lai Sind) bulls.
本试验旨在评价杂交公牛(Brahman × Lai xin, BL)和杂交公牛(Red Angus × Lai xin, AL)的胴体特性和肉品质。在24月龄屠宰前育肥30头、15头/杂交基因型公牛90天。测定了30头屠宰动物的胴体性状和肉质。结果表明:AL公牛的屠宰重、胴体重、胴体屠宰率、腰肌面积均高于BL公牛(p0.05)，但在屠宰后24 h迅速下降(p<0.05)，在贮藏期间维持不显著;肉品的滴漏损失、蒸煮损失和嫩度受基因型的影响，AL公牛的这些值低于BL公牛(p<0.05)。综上所述，红安格斯×赖信杂交公牛的胴体特性和肉质均优于婆罗门×赖信杂交公牛。
{"title":"Carcass characteristics and meat quality of crossbred (Brahman × Lai Sind) and (Red Angus × Lai Sind) bulls kept in small scale farms","authors":"Ngo Thi Kim Chi, Pham The Hue, Tran Quang Hanh, L. D. Ngoan","doi":"10.51227/ojafr.2023.24","DOIUrl":"https://doi.org/10.51227/ojafr.2023.24","url":null,"abstract":"This study aimed to evaluate carcass characteristics and meat quality of cross-bred (Brahman × Lai Sind, BL) bulls and cross-bred (Red Angus × Lai Sind, AL) bulls. A total of 30 bulls, 15 head/crossbred genotype were fattened for 90 days before slaughtering at 24 months of age. Carcass traits and meat quality were accordingly measured in 30 slaughtered animals. Results showed that the slaughter weight, carcass weight, carcass dressing, meat percentage, loin muscle area were higher for AL bulls than for BL bulls (p<0.05). The color of the meat was not affected by genotype with exception of L* at 48, 168 and 336 hours after slaughter, and this value was higher in AL than in BL bulls (p<0.05). The pH of the meat was not different between genotypes (p>0.05) but decreased quickly at 24 hours after slaughter (p<0.05), then maintained not significantly during storage times. The drip loss, cooking loss and tenderness of the meat were affected by cattle genotype and these values were lower in AL bulls than in BL bulls (p<0.05). In conclusion, crossbred (Red Angus × Lai Sind) bulls were higher carcass characteristics, and were better meat quality than crossbred (Brahman × Lai Sind) bulls.","PeriodicalId":19485,"journal":{"name":"Online Journal of Animal and Feed Research","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41990780","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This study aimed to detect Anaplasma phagocytophilum in horses through hematological and molecular tests. The 16S rRNA gene of the Anaplasma phagocytophilum parasite was amplified by polymerase chain reaction (PCR), then sequenced, and subjected to phylogenetic analysis to explore "Equine Granulocytic Anaplasmosis" (EGA) infection in three important gathering race horses areas in Baghdad governorate, Iraq. Blood samples were obtained from 160 horses of varying ages, three breeds, and both sexes, between January and December 2021. Prevalence and risk variables for anaplasmosis were analyzed using statistical odds ratio and chi-square tests. Results demonstrated that clinical anaplasmosis symptoms comprised jaundice, weight loss, paleness of mucus membrane with petechial hemorrhage in the third elides, and edema in extremities; There was no tick infestation. The hematological test did not significantly reveal decreases in red and white blood cells and platelet count. Microscopically found 11 from 160 smears (6.88%) had morulae within granulocytes, PCR results of Anaplasma spp primers was 32 positive amplicons (20%), and molecular sequencing results of “16S ribosomal RNA genes” confirmed 21 horses (13.13%) infected by Anaplasma phagocytophilum for the first time in Iraq horses. The results of the phylogenetic analysis revealed compatibility values similarity 98.81-99.76% with worldwide isolates. Mares occurred not significantly riskier; also age and breed were not illustrated risks of any group. This study is the first molecular detection of Anaplasma phagocytophilum in racehorses reared in Baghdad in Iraq. The outcomes of this study provide genetic data for early identification of Anaplasma phagocytophilum infection, treatment, and management of the illness in Iraq horses, as well as monitoring its transmission to the human population.
{"title":"Phylogenetic identification of Anaplasma phagocytophilum in horses in Baghdad, Iraq","authors":"Ahmed Noaman Alani, A. Yousif","doi":"10.51227/ojafr.2023.25","DOIUrl":"https://doi.org/10.51227/ojafr.2023.25","url":null,"abstract":"This study aimed to detect Anaplasma phagocytophilum in horses through hematological and molecular tests. The 16S rRNA gene of the Anaplasma phagocytophilum parasite was amplified by polymerase chain reaction (PCR), then sequenced, and subjected to phylogenetic analysis to explore \"Equine Granulocytic Anaplasmosis\" (EGA) infection in three important gathering race horses areas in Baghdad governorate, Iraq. Blood samples were obtained from 160 horses of varying ages, three breeds, and both sexes, between January and December 2021. Prevalence and risk variables for anaplasmosis were analyzed using statistical odds ratio and chi-square tests. Results demonstrated that clinical anaplasmosis symptoms comprised jaundice, weight loss, paleness of mucus membrane with petechial hemorrhage in the third elides, and edema in extremities; There was no tick infestation. The hematological test did not significantly reveal decreases in red and white blood cells and platelet count. Microscopically found 11 from 160 smears (6.88%) had morulae within granulocytes, PCR results of Anaplasma spp primers was 32 positive amplicons (20%), and molecular sequencing results of “16S ribosomal RNA genes” confirmed 21 horses (13.13%) infected by Anaplasma phagocytophilum for the first time in Iraq horses. The results of the phylogenetic analysis revealed compatibility values similarity 98.81-99.76% with worldwide isolates. Mares occurred not significantly riskier; also age and breed were not illustrated risks of any group. This study is the first molecular detection of Anaplasma phagocytophilum in racehorses reared in Baghdad in Iraq. The outcomes of this study provide genetic data for early identification of Anaplasma phagocytophilum infection, treatment, and management of the illness in Iraq horses, as well as monitoring its transmission to the human population.","PeriodicalId":19485,"journal":{"name":"Online Journal of Animal and Feed Research","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45840833","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Benlemlih, A. Barchan, A. Aarab, M. Bakkali, A. Arakrak, A. Laglaoui
The objective of this study was to compare the influence of antibiotic and thyme dietary supplements on zootechnical parameters and caecal microflora of growing rabbits. One hundred and ninety eight weaned rabbits (forty days old), white New Zealand (of both sexes) were divided into three groups to submit to the following dietary treatments: Control diet, diet A (control diet + 100 ppm zinc bacitracin), and diet T (control diet + 7% Thymus capitatus leaves) for twenty-one days. The remaining nine days they received only the control diet. The results showed that both the live body weight and feed conversion ratio were positively affected by the antibiotic diet (P<0.05). However, the rabbits' growth performance was not influenced by dietary thyme supplements. The antimicrobial effect of thyme observed against C. perfringens in caecum is not determined even after 20 days of treatment. In conclusion, zootechnical parameters and mortality were not positively affected by dietary thyme supplements comparing it with the antibiotic diet, but these phytobiotics showed the antibacterial effect against E. coli and C. perfringens in caecum of rabbit.
{"title":"Influence of dietary supplementation of antibiotic and thyme on zootechnical parameters and caecal microflora of growing rabbit","authors":"M. Benlemlih, A. Barchan, A. Aarab, M. Bakkali, A. Arakrak, A. Laglaoui","doi":"10.51227/ojafr.2023.29","DOIUrl":"https://doi.org/10.51227/ojafr.2023.29","url":null,"abstract":"The objective of this study was to compare the influence of antibiotic and thyme dietary supplements on zootechnical parameters and caecal microflora of growing rabbits. One hundred and ninety eight weaned rabbits (forty days old), white New Zealand (of both sexes) were divided into three groups to submit to the following dietary treatments: Control diet, diet A (control diet + 100 ppm zinc bacitracin), and diet T (control diet + 7% Thymus capitatus leaves) for twenty-one days. The remaining nine days they received only the control diet. The results showed that both the live body weight and feed conversion ratio were positively affected by the antibiotic diet (P<0.05). However, the rabbits' growth performance was not influenced by dietary thyme supplements. The antimicrobial effect of thyme observed against C. perfringens in caecum is not determined even after 20 days of treatment. In conclusion, zootechnical parameters and mortality were not positively affected by dietary thyme supplements comparing it with the antibiotic diet, but these phytobiotics showed the antibacterial effect against E. coli and C. perfringens in caecum of rabbit.","PeriodicalId":19485,"journal":{"name":"Online Journal of Animal and Feed Research","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44540678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Based on importance of animal products safety, the purpose of this work was to assess the extent of parasitism at the wholesale market level of Inezgane and the fishing port of Agadir in Morocco. For this purpose, fieldwork aimed at direct investigation of parasites involved 366 fish pieces. This study was conducted in the period between March and June 2021. The prevalence of parasitism was 20.76%. The total number of parasites collected is 2385 including 1959 nematodes, 318 xenomas, 92 cestodes, and 16 isopods. An abundance of 6.51 and an overall intensity of 31.38. These infestation parameters varied by species and location of origin. For the qualitative analysis of the parasites, the study revealed a predominance of L3 larvae of the Anisakis nematode with a percentage of 82.14%. Xenomas had a percentage of 13.33%. As for the cestodes of Gymnorhynchus gigas, the larvae were collected from the Atlantic pomfret (Brama brama) with a percentage of 3.86%. As a result of this study, a significant positive correlation of r=0.81 was shown between the total length of the fish and the number of anisakids. The results of this study revealed that the extent of parasitism seems to be less pronounced in some species, but there is still a presence of concern.
{"title":"Occurrence of parasites in fish marketed in the Inezgane wholesale market and the fishing port of Agadir, Morocco","authors":"Said Dahani, N. Bouchriti, Oleya EL HARIRI","doi":"10.51227/ojafr.2023.28","DOIUrl":"https://doi.org/10.51227/ojafr.2023.28","url":null,"abstract":"Based on importance of animal products safety, the purpose of this work was to assess the extent of parasitism at the wholesale market level of Inezgane and the fishing port of Agadir in Morocco. For this purpose, fieldwork aimed at direct investigation of parasites involved 366 fish pieces. This study was conducted in the period between March and June 2021. The prevalence of parasitism was 20.76%. The total number of parasites collected is 2385 including 1959 nematodes, 318 xenomas, 92 cestodes, and 16 isopods. An abundance of 6.51 and an overall intensity of 31.38. These infestation parameters varied by species and location of origin. For the qualitative analysis of the parasites, the study revealed a predominance of L3 larvae of the Anisakis nematode with a percentage of 82.14%. Xenomas had a percentage of 13.33%. As for the cestodes of Gymnorhynchus gigas, the larvae were collected from the Atlantic pomfret (Brama brama) with a percentage of 3.86%. As a result of this study, a significant positive correlation of r=0.81 was shown between the total length of the fish and the number of anisakids. The results of this study revealed that the extent of parasitism seems to be less pronounced in some species, but there is still a presence of concern.","PeriodicalId":19485,"journal":{"name":"Online Journal of Animal and Feed Research","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41324113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ahamidou Moustapha, E. Talaki, A. Akourki, Haladou Gagara
The present study was conducted to determine the prevalence of Newcastle disease and to identify potential risk factors in village chickens in Niger. A total of 1,627 serum samples were collected using a stratified random sampling method with proportional allocation. Samples were collected from village breedings in the departments of the Maradi region (Guidan Roumdji, Madarounfa, Aguié, Gazaoua, and Tessaoua), departments of Zinder region (Takeita, Kantché, Magaria, Dungass, and Mirriah), and cities of Maradi and Zinder. Data on risk factors were collected through an interview with the herders. All collected sera were subjected to competitive enzyme-linked immunosorbent assay (cELISA) to detect Newcastle disease virus-induced antibodies. The findings were indicative of 302 positive sera, representing an overall seroprevalence of 18.6%. The Student’s t-test at p<0.05 revealed a significant difference between regions and among some departments. Furthermore, the logistic regression test identified the agroecological zone, type of breeding, species mix, and the origin of the animals as risk factors associated with seropositivity to Newcastle disease virus. The present results confirmed the exposure of village chickens to the Newcastle disease virus, emphasizing the need to intensify vaccination campaigns and educate poultry farmers on adopting biosecurity measures.
{"title":"Impact and prevalence of Newcastle disease and associated risk factors in village chickens in southern Niger","authors":"Ahamidou Moustapha, E. Talaki, A. Akourki, Haladou Gagara","doi":"10.51227/ojafr.2023.32","DOIUrl":"https://doi.org/10.51227/ojafr.2023.32","url":null,"abstract":"The present study was conducted to determine the prevalence of Newcastle disease and to identify potential risk factors in village chickens in Niger. A total of 1,627 serum samples were collected using a stratified random sampling method with proportional allocation. Samples were collected from village breedings in the departments of the Maradi region (Guidan Roumdji, Madarounfa, Aguié, Gazaoua, and Tessaoua), departments of Zinder region (Takeita, Kantché, Magaria, Dungass, and Mirriah), and cities of Maradi and Zinder. Data on risk factors were collected through an interview with the herders. All collected sera were subjected to competitive enzyme-linked immunosorbent assay (cELISA) to detect Newcastle disease virus-induced antibodies. The findings were indicative of 302 positive sera, representing an overall seroprevalence of 18.6%. The Student’s t-test at p<0.05 revealed a significant difference between regions and among some departments. Furthermore, the logistic regression test identified the agroecological zone, type of breeding, species mix, and the origin of the animals as risk factors associated with seropositivity to Newcastle disease virus. The present results confirmed the exposure of village chickens to the Newcastle disease virus, emphasizing the need to intensify vaccination campaigns and educate poultry farmers on adopting biosecurity measures.","PeriodicalId":19485,"journal":{"name":"Online Journal of Animal and Feed Research","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47650272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Andi Ikhsan Wijaya, Ismartoyo Ismartoyo, A. Natsir
The quality of feed given to ruminants can be determined from the degradation of nutrient content in the rumen. This study aimed to determine the pattern of forage degradation and the characteristics degradation of crude protein in the rumen using the in sacco method. The study used 4 fistulae kacang goats with an average body weight of 14.57 kg. The forage used consisted of R1: elephant grass (Pennisetum purpureum), R2: mini elephant grass (Pennisetum purpureum cv. Mott), R3: guinea grass (Panicum maximum), and R4: signal grass (Bracharia decumbens). The nylon bag is made of polyester measuring 8x4 cm with a porosity of 40 µm. Feed samples were put into the rumen and incubated for 0, 4, 8, 12, 24, 48, and 72 hours. The parameters measured were consumption, patterns, and forage degradation characteristics by calculating the values of a, b, c, a+b, lag time, and ED. Determination characteristics of feed degradation in the rumen by in sacco method will be analyzed. The results showed that the characteristics of crude protein degradation had significant differences in fraction values a, b, a+b, and lag time (P<0.05), while c and ED did not have significant differences (P>0.05). In conclusion the crude protein of the degradation characteristics in the rumen were: elephant grass (a: 9.88%, b: 64.37%, and c: 0.06/h-1), mini elephant grass (a: 16.50%, b: 45.24%, and c: 0.05/h-1), guinea grass (a: 7.42%, b: 68.24%, and c: 0.05/h-1), and signal grass (a: 6.79%, b: 56.19%, and c: 0.07/h-1). So, grass can provide sufficient protein for microbial growth in the rumen for ruminants.
{"title":"Analysis of rumen degradation characteristics of forage crude protein in goat","authors":"Andi Ikhsan Wijaya, Ismartoyo Ismartoyo, A. Natsir","doi":"10.51227/ojafr.2023.33","DOIUrl":"https://doi.org/10.51227/ojafr.2023.33","url":null,"abstract":"The quality of feed given to ruminants can be determined from the degradation of nutrient content in the rumen. This study aimed to determine the pattern of forage degradation and the characteristics degradation of crude protein in the rumen using the in sacco method. The study used 4 fistulae kacang goats with an average body weight of 14.57 kg. The forage used consisted of R1: elephant grass (Pennisetum purpureum), R2: mini elephant grass (Pennisetum purpureum cv. Mott), R3: guinea grass (Panicum maximum), and R4: signal grass (Bracharia decumbens). The nylon bag is made of polyester measuring 8x4 cm with a porosity of 40 µm. Feed samples were put into the rumen and incubated for 0, 4, 8, 12, 24, 48, and 72 hours. The parameters measured were consumption, patterns, and forage degradation characteristics by calculating the values of a, b, c, a+b, lag time, and ED. Determination characteristics of feed degradation in the rumen by in sacco method will be analyzed. The results showed that the characteristics of crude protein degradation had significant differences in fraction values a, b, a+b, and lag time (P<0.05), while c and ED did not have significant differences (P>0.05). In conclusion the crude protein of the degradation characteristics in the rumen were: elephant grass (a: 9.88%, b: 64.37%, and c: 0.06/h-1), mini elephant grass (a: 16.50%, b: 45.24%, and c: 0.05/h-1), guinea grass (a: 7.42%, b: 68.24%, and c: 0.05/h-1), and signal grass (a: 6.79%, b: 56.19%, and c: 0.07/h-1). So, grass can provide sufficient protein for microbial growth in the rumen for ruminants.","PeriodicalId":19485,"journal":{"name":"Online Journal of Animal and Feed Research","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43466735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The experiment investigated the effects of various soya bean groups (boiled, fermented, and roasted) on Japanese quail at 3 weeks old. 160 Japanese quail were randomly assigned to four treatments (control, boiling soya beans, fermented soya beans, roasted soya beans) with four duplicates each. The 12-week trial lasted. Data on weekly body weights and feed conversion ratio were analyzed using analysis of variance (ANOVA) and Tukey's honestly significant at 5% probability test. The result shows there are significant differences in weekly weights of Japanese quail at weeks 1(828.12-1083.24g), 2(1026.47-1362.02g), and 3(1325.69-1528.20g) with the highest observed in birds in treatment 2(boiled soya beans). The maximum FCR was in week 1 for all treatments, while the lowest was in treatment 3 for weeks 5 and 9 (0.83; P<0.005). Week 1 to week 12 feed conversion ratio decreases. The birds' feed conversion ratios varied significantly (P<0.05). The quails in treatment 4 (roasted soybeans) had the greatest weekly weight after the trial (1742.34g). Thus, quails in treatment 3 (roasted soybean) had the best development performance than the control, boiled and fermented. So it advised that roasted soybean can be an efficient diet for Japanese quails for maximum performance.
{"title":"Effect of three different processing techniques of soybean on nutritional and growth performance of Japanese quail (Coturnix japonica)","authors":"","doi":"10.51227/ojafr.2023.19","DOIUrl":"https://doi.org/10.51227/ojafr.2023.19","url":null,"abstract":"The experiment investigated the effects of various soya bean groups (boiled, fermented, and roasted) on Japanese quail at 3 weeks old. 160 Japanese quail were randomly assigned to four treatments (control, boiling soya beans, fermented soya beans, roasted soya beans) with four duplicates each. The 12-week trial lasted. Data on weekly body weights and feed conversion ratio were analyzed using analysis of variance (ANOVA) and Tukey's honestly significant at 5% probability test. The result shows there are significant differences in weekly weights of Japanese quail at weeks 1(828.12-1083.24g), 2(1026.47-1362.02g), and 3(1325.69-1528.20g) with the highest observed in birds in treatment 2(boiled soya beans). The maximum FCR was in week 1 for all treatments, while the lowest was in treatment 3 for weeks 5 and 9 (0.83; P<0.005). Week 1 to week 12 feed conversion ratio decreases. The birds' feed conversion ratios varied significantly (P<0.05). The quails in treatment 4 (roasted soybeans) had the greatest weekly weight after the trial (1742.34g). Thus, quails in treatment 3 (roasted soybean) had the best development performance than the control, boiled and fermented. So it advised that roasted soybean can be an efficient diet for Japanese quails for maximum performance.","PeriodicalId":19485,"journal":{"name":"Online Journal of Animal and Feed Research","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43823094","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Rina Putri, S. P. Dewi, Fariz Am Kurniawan, M. Ridla, Y. Retnani
This experiment was conducted to evaluate the physical properties, chemical quality, and digestibility of the ammoniated Bambara groundnut (Vigna subterranea) shell as ruminant feed. Bambara groundnut shell (BGS) were collected, ground with a grinder machine, afterward added 0, 3 and 5 (% DM) urea levels into 500 g of sample. Samples were mixed until homogeneous, then put into plastic bottles, after that stored for 7 and 14 days. Opened, dried in the oven at 650C for 48 hours and ground. A completely randomized design (CRD) was used with 5 treatments of BGS ammoniation (T0= control, T1= BGS + 3% urea and 7 days storage, T2= BGS +5% urea and 7 days storage, T3= BGS + 3% urea and 14 days storage, and T4= BGS + 5% urea and 14 days storage time), 4 replications each. The result of this study showed that the increase of urea level and days storage time, can decrease crude fiber, neutral detergent fiber, acid detergent fiber, and hemicellulose contents of all samples (P<0.05) and increase the value of bulk density, tapped density, in vitro dry matter digestibility and in vitro organic matter digestibility in comparison to untreated samples (P<0.05). It was concluded that the T4 was the best treatment. The BGS ammoniated with 5% urea for a period 14 days of storage causes the lowest value of crude fiber, neutral detergent fiber, acid detergent fiber, and hemicellulose, and also causes the highest value of bulk density, tapped density, crude protein, in vitro dry matter digestibility and in vitro organic matter.
{"title":"Physico-chemical properties and digestibility of ammoniated Bambara groundnut (Vigna subterranea) shell for ruminants","authors":"Rina Putri, S. P. Dewi, Fariz Am Kurniawan, M. Ridla, Y. Retnani","doi":"10.51227/ojafr.2023.16","DOIUrl":"https://doi.org/10.51227/ojafr.2023.16","url":null,"abstract":"This experiment was conducted to evaluate the physical properties, chemical quality, and digestibility of the ammoniated Bambara groundnut (Vigna subterranea) shell as ruminant feed. Bambara groundnut shell (BGS) were collected, ground with a grinder machine, afterward added 0, 3 and 5 (% DM) urea levels into 500 g of sample. Samples were mixed until homogeneous, then put into plastic bottles, after that stored for 7 and 14 days. Opened, dried in the oven at 650C for 48 hours and ground. A completely randomized design (CRD) was used with 5 treatments of BGS ammoniation (T0= control, T1= BGS + 3% urea and 7 days storage, T2= BGS +5% urea and 7 days storage, T3= BGS + 3% urea and 14 days storage, and T4= BGS + 5% urea and 14 days storage time), 4 replications each. The result of this study showed that the increase of urea level and days storage time, can decrease crude fiber, neutral detergent fiber, acid detergent fiber, and hemicellulose contents of all samples (P<0.05) and increase the value of bulk density, tapped density, in vitro dry matter digestibility and in vitro organic matter digestibility in comparison to untreated samples (P<0.05). It was concluded that the T4 was the best treatment. The BGS ammoniated with 5% urea for a period 14 days of storage causes the lowest value of crude fiber, neutral detergent fiber, acid detergent fiber, and hemicellulose, and also causes the highest value of bulk density, tapped density, crude protein, in vitro dry matter digestibility and in vitro organic matter.","PeriodicalId":19485,"journal":{"name":"Online Journal of Animal and Feed Research","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45987417","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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