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Kaolin foliar spray induces positive modifications in volatile compounds and fruit quality of Touriga-Nacional red wine 高岭土叶面喷施对图里加-纳西尔(Touriga-Nacional)红葡萄酒的挥发性化合物和果实质量产生积极影响
IF 2.9 3区 农林科学 Q1 Agricultural and Biological Sciences Pub Date : 2024-04-19 DOI: 10.20870/oeno-one.2024.58.2.7945
L. Dinis, Sandra Pereira, Irene Fraga, Sílvia M. Rocha, Carina Costa, Cátia Martins, Alice Vilela, Margarida Arrobas, J. Moutinho-Pereira
Solar radiation and temperature play crucial roles in grapevine metabolic processes and are known to have a positive impact on grape berry composition; however, excessive exposure to these factors can be detrimental. Kaolin-based particle film technology has emerged as a valuable solution for mitigating the effects of heat and water stresses in vineyards. This work aimed to evaluate the effects of kaolin application on the phenolic composition, chromatic characteristics, oenological parameters, volatile compounds and sensory profile of red wine. The study was carried out in one growing season in the commercial vineyard "Quinta dos Aciprestes" in the Douro Superior sub-region. Twelve rows in triplicate of the Touriga-Nacional variety underwent foliar kaolin treatment (applied at the pre-veraison stage, at the manufacturer-recommended and -tested dosage of 5 % (w/v)), and another twelve rows comprised the non-treated control group. Kaolin increased the phenolic compound and tartaric acid concentrations (2.4 % and 20.8 % respectively), total acidity (2.4 %), the deep reddish colour of the berries, total and coloured anthocyanin (2.8 %), and total and polymeric pigments (3.6 %); meanwhile, a decrease was observed in pH (-1.4 %) and alcoholic degree (-4.8 %). No significant differences were observed in any sensory parameters between the wine from the kaolin-treated and control vines, but the tasters found the aroma of the former to be fruitier and more complex, with an agreeable acidic taste and persistence. It was possible to group the volatile compounds into two distinct groups based on the results of the Pearson’s correlation matrix. This grouping corresponds to the sensory descriptors common to each of the respective volatiles. Overall, the results further support the potential efficacy of utilising kaolin to alleviate summer-related stress in grapevines.
太阳辐射和温度在葡萄树的新陈代谢过程中起着至关重要的作用,对葡萄浆果的成分也有积极的影响。基于高岭土的颗粒薄膜技术已成为减轻葡萄园热量和水分胁迫影响的重要解决方案。这项工作旨在评估高岭土的应用对红葡萄酒的酚类成分、色度特征、酿酒参数、挥发性化合物和感官特征的影响。研究在杜罗河上游次地区的商业葡萄园 "Quinta dos Aciprestes "的一个生长季节内进行。对一式三份的 12 行 Touriga-Nacional 品种进行了叶面高岭土处理(在葡萄成熟前施用,按生产商建议和测试的 5 %(w/v)用量),另外 12 行为未处理对照组。高岭土提高了酚类化合物和酒石酸的浓度(分别为 2.4 % 和 20.8 %)、总酸度(2.4 %)、浆果的深红色、总花青素和有色花青素(2.8 %)以及总色素和聚合色素(3.6 %);同时,pH 值(-1.4 %)和酒精度(-4.8 %)有所下降。经高岭土处理过的葡萄酒和对照组的葡萄酒在感官参数上没有明显差异,但品酒师认为前者的香气更果香、更复杂,酸味宜人,且持久。根据皮尔逊相关矩阵的结果,可以将挥发性化合物分为两组。这种分组与每种挥发性化合物共同的感官描述符相对应。总之,这些结果进一步证明了利用高岭土缓解葡萄树夏季相关压力的潜在功效。
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引用次数: 0
Overhead spray water treatment as a mitigation strategy to alleviate vine stress and safeguard grape quality during heatwaves 高空喷水处理作为一种缓解策略,可在热浪期间减轻葡萄树的压力并保障葡萄质量
IF 2.9 3区 农林科学 Q1 Agricultural and Biological Sciences Pub Date : 2024-04-16 DOI: 10.20870/oeno-one.2024.58.2.7847
Alena Wilson, Marta Dizy, Deolindo Dominguez, María Inés de Rosas, Yésica Baldo, Luciana Garcia, R. Gargantini, Leonor Deis, Liliana Martinez
Changes in climate are influencing the quality of wine grapes worldwide. The impact of extreme climate events over short periods is increasingly recognised as a serious risk to grape quality and yield quantity. In this study, the mitigation effects of a pulsed water spray treatment on vine canopy during heatwave (HW) events were evaluated in relation to vine condition during the growing season and grape quality. In the UNCuyo experimental vineyard, vines of the three cultivars Malbec (ML), Bonarda (BO) and Syrah (SY) were treated with an overhead pulsed water spray. Heatwaves were defined as days with a minimum temperature of 21 °C and a maximum temperature of 35 °C. Two heat waves were identified during the growing season. Samples were collected at weekly intervals from veraison to harvest. On five sample dates, Leaf and Stem Water Potential (LWP, SWP), Stomatal Conductance (gs), Leaf Temperature (LT), Berry Temperature (BT), Chlorophyll Content (CC), Fluorescence (Fv/Fm) and Performance Index (PI) were recorded at several time points during the day to evaluate the physiological responses of the vine. Berries were collected on each sample date and at harvest. Berry weight, soluble solid content and pH were recorded. In the treated vines (Trt), LWP, SWP, Fv/Fm, PI and gs were significantly higher and LT was lower than in the control vines (Ctl) during the second heatwave, which was longer and more intense than the first one. One week after the more severe heatwave, LWP, SWP and gs remained significantly higher in Trt than in Ctl, displaying reduced physiological stress in Trt. At harvest, the anthocyanin profile, total polyphenol index (TPI), fruit yield, number of bunches and their average weight, berry weight, soluble solid content and pH were also recorded. Bunch weight was significantly higher in Trt for all cultivars. No differences were found in total anthocyanin concentration. These findings indicate that the vines subjected to targeted overhead water treatment during heatwaves underwent less physiological stress and yielded higher grape production, without increasing the risk of potential fungus diseases, in the Mendoza climate. Consequently, this practice could serve as a valuable strategy for mitigating the adverse effects of heatwaves.
气候的变化正在影响世界各地酿酒葡萄的质量。人们越来越认识到,短时间内极端气候事件的影响对葡萄的质量和产量构成严重威胁。本研究评估了热浪(HW)事件期间脉冲喷水处理对葡萄树冠层的缓解作用,并将其与葡萄树在生长季节的状况和葡萄质量联系起来。在 UNCuyo 试验葡萄园中,对马尔贝克(ML)、博纳达(BO)和西拉(SY)三个品种的葡萄树进行了高空脉冲喷水处理。热浪的定义是最低温度为 21 °C,最高温度为 35 °C。生长季中出现了两次热浪。从成熟期到收获期,每周采集一次样本。在五个采样日,在一天中的多个时间点记录叶片和茎干水势(LWP、SWP)、气孔导度(gs)、叶片温度(LT)、浆果温度(BT)、叶绿素含量(CC)、荧光(Fv/Fm)和性能指数(PI),以评估葡萄树的生理反应。在每个采样日期和收获时收集浆果。记录浆果重量、可溶性固体含量和 pH 值。与对照组(Ctl)相比,在第二次热浪期间,经过处理的葡萄树(Trt)的 LWP、SWP、Fv/Fm、PI 和 gs 显著升高,而 LT 则降低。更猛烈的热浪过后一周,Trt 的 LWP、SWP 和 gs 仍明显高于 Ctl,这表明 Trt 的生理压力有所减轻。收获时,还记录了花青素含量、总多酚指数(TPI)、果实产量、果串数及其平均重量、浆果重量、可溶性固形物含量和 pH 值。在所有栽培品种中,Trt 的果穗重量明显较高。总花青素浓度没有差异。这些研究结果表明,在门多萨的气候条件下,热浪期间对葡萄树进行有针对性的高架水处理,可以减少葡萄树的生理压力,提高葡萄产量,同时不会增加潜在真菌疾病的风险。因此,这种做法可以作为减轻热浪不利影响的宝贵策略。
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引用次数: 0
UV-C-induced changes in a white wine: Evaluating the protective power of hydrolysable tannins and SO2 紫外线诱导的白葡萄酒变化:评估可水解单宁和二氧化硫的保护能力
IF 2.9 3区 农林科学 Q1 Agricultural and Biological Sciences Pub Date : 2024-04-03 DOI: 10.20870/oeno-one.2024.58.1.7697
Svetlana Cvetkova, Michael Wacker, Jutta Keiser, Benedikt Hirt, Mario Stahl, M. Scharfenberger-Schmeer, Dominik Durner
UV‑C treatment is a non-thermal technology that could be used for the microbiological stabilisation of must and wine. However, light - in this case UV‑C light - has an impact on wine. UV‑C is a high-energy light source and can promote photo-induced oxidation reactions. The formation of reactive oxygen species during the UV-C treatment of wine can be prevented via supplementation with antioxidants. These have the ability to neutralise reactive oxygen species or directly interact with the products of photo-induced oxidation reactions. This study investigates the impact of different UV‑C doses on the chemical and sensory characteristics of Chardonnay wine and examines the protective effect of two antioxidants: sulphur dioxide (SO2) and hydrolysable tannins. A sensory evaluation revealed that UV‑C doses higher than 1 kJ/l increased the colour intensity and the oxidised and burnt odour attributes of the wine. The peach odour attribute decreased with UV‑C treatment. On a chemical level, increasing UV‑C doses promoted the formation of compounds such as acetaldehyde and 2‑aminoacetophenone (2‑AAP), while causing the degradation of C13‑norisoprenoids, higher alcohols, monoterpenes and esters. Contrary to expectations, SO2 did not act as an antioxidant when wine was treated with UV-C. It intensified the burnt odour attribute and did not mitigate the intensity of the oxidised odour attribute, which can be explained by the formation of acetaldehyde. However, hydrolysable tannins acted as antioxidants when combined with UV‑C treatment, effectively mitigating the formation of 2‑AAP and acetaldehyde, while preventing the wine from becoming oxidised.
紫外线-C 处理是一种非热处理技术,可用于葡萄汁和葡萄酒的微生物稳定。然而,光(这里指 UV-C 光)对葡萄酒有影响。紫外线-C 是一种高能光源,可促进光诱导氧化反应。可以通过补充抗氧化剂来防止葡萄酒在紫外线-C 处理过程中形成活性氧。抗氧化剂具有中和活性氧或直接与光诱导氧化反应产物相互作用的能力。本研究调查了不同紫外线-C 剂量对霞多丽葡萄酒化学和感官特性的影响,并检验了两种抗氧化剂:二氧化硫(SO2)和可水解单宁的保护作用。感官评估显示,紫外线 C 剂量高于 1 kJ/l 会增加葡萄酒的颜色强度以及氧化和焦味属性。桃子气味会随着紫外线-C 处理的增加而减少。在化学层面上,紫外线-C 剂量的增加促进了乙醛和 2-氨基苯乙酮(2-AAP)等化合物的形成,同时导致了 C13-异戊烯、高级醇、单萜烯和酯的降解。与预期相反,用紫外线-C 处理葡萄酒时,二氧化硫并没有起到抗氧化剂的作用。它加剧了焦味,但并没有减轻氧化味的强度,这可以用乙醛的形成来解释。然而,可水解单宁在与紫外线-C 处理相结合时起到了抗氧化剂的作用,有效缓解了 2-AAP 和乙醛的形成,同时防止了葡萄酒氧化。
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引用次数: 0
Quantitative analysis of sugars extracted from French oak (Quercus petraea) chips: Effect of toasting temperature 从法国橡木(Quercus petraea)木片中提取的糖的定量分析:烘烤温度的影响
IF 2.9 3区 农林科学 Q1 Agricultural and Biological Sciences Pub Date : 2024-04-02 DOI: 10.20870/oeno-one.2024.58.1.7755
Genki Kainuma, Ayano Mochizuki, Fumie Watanabe-Saito, Masashi Hisamoto, Gilles De Revel, Tohru Okuda
In this study, we conducted extraction experiments using model wine on French oak chips toasted at various temperatures to investigate the extracted sugars. A certain amount of sugars were extracted from the oak chips, and the quantity significantly varied depending on the toasting temperature. Analysis of the composition of the extracted sugars revealed the presence of substantial amounts of xylose and glucose, with the composition showing significant differences based on the toasting temperature. Furthermore, it was elucidated that the extracted sugars included monosaccharides and oligo or polysaccharides. This research represents the first comprehensive analysis correlating the toasting temperature with the extraction of a substantial amount of sugars from toasted chips.
在这项研究中,我们使用在不同温度下烘烤的法国橡木片上的模型葡萄酒进行了萃取实验,以研究萃取出的糖分。我们从橡木片中提取了一定量的糖分,其数量随烘烤温度的不同而有显著变化。对提取的糖类成分进行分析后发现,其中含有大量的木糖和葡萄糖,且其成分随烘烤温度的不同而有显著差异。此外,还阐明了提取的糖类包括单糖和寡糖或多糖。这项研究首次全面分析了烘烤温度与从烘烤薯片中提取大量糖类的关系。
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引用次数: 0
Stimulation of secondary metabolism in grape berry exocarps by a nature-based strategy of foliar application of polyols 通过叶面喷施多元醇的自然策略刺激葡萄浆果外果皮的次生代谢
IF 2.9 3区 农林科学 Q1 Agricultural and Biological Sciences Pub Date : 2024-03-25 DOI: 10.20870/oeno-one.2024.58.1.7537
Artur Conde, Hélder Badim, L. Dinis, J. Moutinho-Pereira, Manon Ferrier, Marianne Unlubayir, Arnaud Lanoue, H. Gerós
In grapes under drought stress, polyols accumulate through tight coordination at the molecular level between increased membrane transport of polyols and inhibition of polyol oxidation. Here, the effects on grape metabolism of an exogenous foliar application of polyols as a potential sustainable viticultural practice to increase grapevine performance and berry quality were thoroughly assessed. Grapevines were pulverised with a polyol solution containing 2 mM mannitol and 2 mM sorbitol, and the metabolome of grape berry exocarps and important metabolic pathways associated with berry quality and polyol metabolism were analysed at véraison and mature stages. By combining metabolomics analysis using UPLC-MS, enzyme activity assays and targeted transcriptional analyses, it was demonstrated that foliar application of polyols stimulated by 3.5-fold and 6-fold abscisic acid (ABA) biosynthesis at véraison and mature grape berries, respectively. It also stimulated the concentration of anthocyanins, stilbenes and total phenolics in exocarps through the upregulation of phenylpropanoid, stilbenoid and anthocyanin biosynthetic pathways shown by increases in phenylalanine-ammonia lyase (PAL) activity (3-fold) and VviPAL1 expression, stilbene synthase 1 (VviSTS1) transcripts (ca. 5-fold), UDP-glucose:flavonoid 3-O-glucosyltransferase (UFGT) activity and VviUFGT1 expression, among other results, at the mature stage, when these changes were most noticeable. Many secondary metabolites synthesised in these pathways identified by UPLC-MS analysis were present at higher quantities in exocarps from polyol-treated plants such as fertaric acid, E-resveratrol, E-piceatannol, piceid, pallidol, E-ε-viniferin, myricetin-hexoside 1, cyanidin-3-O-glucoside and malvidin-3-O-(6-p-coumaroyl)-glucoside. Foliar application of low-concentration polyols is, therefore, a promising biostimulant-based strategy to improve grape berry quality and nutritional value in the current context of climate change.
在干旱胁迫下的葡萄中,多元醇会通过增加多元醇的膜运输和抑制多元醇氧化之间在分子水平上的紧密协调而积累。在此,我们全面评估了外源叶面喷施多元醇对葡萄新陈代谢的影响,这是一种潜在的可持续葡萄栽培方法,可提高葡萄树的性能和浆果质量。用含有 2 毫摩尔甘露醇和 2 毫摩尔山梨醇的多元醇溶液粉碎葡萄藤,并在葡萄成熟期和成熟阶段分析葡萄浆果外果皮的代谢组以及与浆果质量和多元醇代谢相关的重要代谢途径。通过结合使用 UPLC-MS 进行代谢组学分析、酶活性测定和靶向转录分析,结果表明叶面喷施多元醇可分别刺激葡萄果实成熟期和成熟期脱落酸(ABA)生物合成的 3.5 倍和 6 倍。它还通过上调苯丙氨酸、芪类和花青素生物合成途径,刺激了外果皮中花青素、芪类和总酚的浓度,具体表现为苯丙氨酸氨裂解酶(PAL)活性(3 倍)和 VviPAL1 表达量、芪类合成酶 1(VviSTS1)转录本(约 5 倍)、UDP-gP1 表达量和 VviPAL1 表达量的增加。在成熟阶段,这些变化最为明显,其中包括 UDP-葡萄糖:类黄酮 3-O-葡萄糖基转移酶(UFGT)活性和 VviUFGT1 表达。通过 UPLC-MS 分析确定的在这些途径中合成的许多次生代谢物在多元醇处理过的植物外果皮中含量较高,如肥料酸、E-藜芦醇、E-苦橙皮酚、苦橙皮苷、苍白酚、E-ε-维尼芬素、杨梅素-己糖苷 1、青花素-3-O-葡萄糖苷和麦饭石素-3-O-(6-对香豆酰)-葡萄糖苷。因此,在当前气候变化的背景下,叶面喷施低浓度多元醇是一种很有前景的生物刺激策略,可提高葡萄浆果的质量和营养价值。
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引用次数: 0
Improved berry and wine quality of Vitis vinifera L. cv. Gewürztraminer grown in an arid climate using a Y-shaped training system 利用 Y 型培训系统提高干旱气候条件下种植的葡萄品种琼瑶浆的浆果和葡萄酒质量
IF 2.9 3区 农林科学 Q1 Agricultural and Biological Sciences Pub Date : 2024-03-12 DOI: 10.20870/oeno-one.2024.58.1.7148
Yaara Zohar, K. Reta, E. Drori, Udi Gliksman, Shiki Rauchberger, Einat Bar, E. Lewinsohn, Nurit Agam, Aaron Fait
Recent global climatic changes have highlighted viticulture in arid/semiarid regions as an increasingly relevant study model. Grapes in arid regions face excessive solar irradiance, leading to more than 50 °C berry surface temperatures in exposed berries. The resultant oxidative stress, sunburn necrosis, and browning consistently reduce berry quality. Adapting an adequate training system to the climate and cultivar is a simple and inexpensive method to control the radiation regime. This study compares the berry and wine characteristics and compositional components of desert-grown Vitis vinifera L. cv. Gewürztraminer, trained on either a Vertical Shoot Positioning (VSP) system or a Y-shaped training system (SAYM, Sistema di Allevamendo ad Ypsilon Integralmente Meccanizzabile), a method with a higher canopy light interception. The SAYM training reduced direct radiation and concomitant heat in the cluster zone and significantly alleviated oxidative stress in berries in the 2016/17 season. In addition, SAYM-grown berries were preferable in terms of quality and productivity. Correspondingly, a sensorial analysis rated SAYM wines higher than VSP wines in all categories. Photosynthetic pigment content in the berries' skin showed similarities between VSP and SAYM, and a volatile compound analysis of the wines by GC-MS revealed a higher ester content in the wines derived from SAYM vines, along with a higher content of compounds linked to Gewürztraminer wine typicity (varietal characteristics such as esters, terpenoids, and alcohols).
最近的全球气候变化突出表明,干旱/半干旱地区的葡萄栽培是一个日益相关的研究模型。干旱地区的葡萄面临过度的太阳辐照,导致暴露在阳光下的浆果表面温度超过 50 °C。由此产生的氧化应激、日灼坏死和褐变不断降低浆果的品质。根据气候和栽培品种调整适当的培训系统是控制辐射制度的一种简单而廉价的方法。本研究比较了在沙漠中种植的葡萄品种琼瑶浆的浆果和葡萄酒特征及组成成分,它们分别采用了垂直嫩枝定位系统(VSP)或 Y 型栽培系统(SAYM,Sistema di Allevamendo ad Ypsilon Integralmente Meccanizzabile)进行栽培,Y 型栽培系统具有更高的冠层截光能力。SAYM 栽培法减少了直接辐射和果穗区随之而来的热量,并显著减轻了 2016/17 榨季浆果的氧化应激。此外,SAYM 法种植的浆果在质量和产量方面更胜一筹。相应地,感官分析对 SAYM 葡萄酒的各项评分均高于 VSP 葡萄酒。浆果表皮中的光合色素含量显示出 VSP 和 SAYM 的相似性,通过 GC-MS 对葡萄酒进行的挥发性化合物分析表明,SAYM 葡萄园出产的葡萄酒中酯含量更高,与琼瑶浆葡萄酒类型相关的化合物含量也更高(如酯类、萜烯类和醇类等品种特征)。
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引用次数: 0
New insights about sensory contribution of grape stems during winemaking: role of astilbin, a sweet polyphenol 对酿酒过程中葡萄梗感官贡献的新认识:甜多酚 Astilbin 的作用
IF 2.9 3区 农林科学 Q1 Agricultural and Biological Sciences Pub Date : 2024-03-07 DOI: 10.20870/oeno-one.2024.58.1.7872
Marie Le Scanff, Axel Marchal
In the usual red winemaking process, grapes are destemmed between the harvest and the filling of the vat. However, in regions like Bourgogne, some winemakers let all or a part of the stems in contact with the juice during vatting. This choice will likely affect the sensory properties of wine, such as its gustatory perception. The present study investigated the effect of adding stems during the winemaking process on the concentration of a sweetening compound, astilbin. The sensory contribution of astilbin in wines was first clarified by measuring its taste detection threshold and comparing it with the concentrations found in various commercial wines. Then, experimental wines resulting from the addition of stems in various proportions were analysed. These practical experiments were carried out in various French wine estates, in Bourgogne, Beaujolais and Bordeaux, over three vintages, allowing the comparison of different grape varieties, namely Pinot noir, Gamay and Merlot. For each experiment, the modality with the addition of stems was compared with a vat of destemmed grapes from the same plot. Samples were taken throughout the winemaking process to be analysed by liquid chromatography coupled with high-resolution mass spectrometry (UHPLC-Exactive, Orbitrap analyser). The results showed that the addition of stems during vatting significantly increased astilbin concentration. Furthermore, this increase varied according to the grape variety. Whereas the astilbin contents were lower in Merlot wines than in Pinot noir or Gamay wines, the ratio between its concentration of wines from the two modalities was higher in Merlot than in Pinot noir and Gamay. The localisation of astilbin in the different components of the bunch, depending on the grape variety, was also investigated to understand this difference better. Thus, a higher abundance of astilbin in stems than in skins was found in the Merlot variety, whereas, for Gamay and Pinot noir, the total quantity of astilbin in a bunch was located in equal proportions in stems and skins. These new results bring new tools to understand better the practice of whole bunch vinification from a chemical perspective.
在通常的红葡萄酒酿造过程中,葡萄会在采摘和装桶之间进行去梗处理。不过,在勃艮第等地区,一些酿酒师会在装桶过程中让全部或部分葡萄梗与果汁接触。这种选择可能会影响葡萄酒的感官特性,如口感。本研究调查了在酿酒过程中添加葡萄梗对甜味化合物天人菊素浓度的影响。首先,通过测量淀粉糖浆的味觉检测阈值,并将其与各种商业葡萄酒中的浓度进行比较,明确了淀粉糖浆在葡萄酒中的感官贡献。然后,对添加了不同比例茎叶的试验性葡萄酒进行了分析。这些实际实验在法国勃艮第、博若莱和波尔多的多个葡萄酒庄园进行,历时三个年份,比较了不同的葡萄品种,即黑皮诺、佳美和梅洛。在每次实验中,都将添加葡萄梗的方式与来自同一地块的一桶去梗葡萄进行比较。在整个酿酒过程中采集样品,通过液相色谱-高分辨质谱法(UHPLC-Exactive,Orbitrap 分析仪)进行分析。结果表明,在酿酒过程中加入葡萄梗会显著增加天青素的浓度。而且,这种增加因葡萄品种而异。虽然梅洛葡萄酒中的芪含量低于黑比诺或佳美葡萄酒,但梅洛葡萄酒中的芪含量比值高于黑比诺和佳美葡萄酒。为了更好地理解这一差异,我们还研究了不同葡萄品种的葡萄串中鞣花青素不同成分的定位情况。因此,在梅洛品种中,茎中的淀粉样蛋白含量高于皮中,而在佳美和黑皮诺中,葡萄串中淀粉样蛋白的总量在茎和皮中的比例相同。这些新结果为从化学角度更好地理解整串葡萄酿造工艺提供了新的工具。
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引用次数: 0
Enhancing fermentation performance through the reutilisation of wine yeast lees 通过再利用葡萄酒酵母糟提高发酵性能
IF 2.9 3区 农林科学 Q1 Agricultural and Biological Sciences Pub Date : 2024-03-06 DOI: 10.20870/oeno-one.2024.58.1.7749
Cristobal A. Onetto, Jane McCarthy, Mark Solomon, A. Borneman, S. Schmidt
Extensive research has been dedicated to elucidating the role of various nitrogen sources, nitrogen concentrations and the timing of addition when modulating grape must fermentation using yeast. The wine industry invests substantial resources in both the vineyard and the winery to provide adequate nitrogen concentrations for fermentation. This approach ensures optimal yeast performance during fermentation and minimises the risk of negative sensory attributes associated with poor ferment nutrition. In addition to wine, the winemaking process produces a substantial quantity of nutrient-rich biomass, a poorly explored resource that, if appropriately recycled, could be used to support the nutrient requirements of other winery fermentations. This study explored the feasibility of using processed yeast lees generated during alcoholic fermentation as a nutrient supplement in subsequent fermentations. Three lees treatment options were assessed: accelerated autolysis, enzymatic lysis and mechanical lysis. The ability of these treatments to achieve complete lysis of yeast cells and release amino acids and trace elements is reported. The addition of processed lysates into grape juice was shown to improve fermentation timeframes and influence the production of yeast-derived fermentation volatile compounds in a dose-dependent manner. This study demonstrates that recycling spent lees from winery waste is feasible and provides some strategies for extracting nutrients from winery waste.
大量研究致力于阐明使用酵母调节葡萄汁发酵时各种氮源、氮浓度和添加时间的作用。葡萄酒行业在葡萄园和酿酒厂都投入了大量资源,为发酵提供充足的氮浓度。这种方法可确保酵母在发酵过程中发挥最佳性能,并将因发酵营养不良而产生负面感官特性的风险降至最低。除葡萄酒外,酿酒过程中还会产生大量富含营养物质的生物质,这是一种尚未被充分开发的资源,如能适当回收利用,可用于满足其他酒厂发酵对营养物质的需求。本研究探讨了将酒精发酵过程中产生的处理过的酵母糟作为后续发酵的营养补充物的可行性。对三种酒糟处理方案进行了评估:加速自溶、酶溶解和机械溶解。报告显示,这些处理方法都能实现酵母细胞的完全裂解,并释放出氨基酸和微量元素。研究表明,在葡萄汁中加入处理过的裂解物可缩短发酵时间,并以剂量依赖的方式影响酵母衍生发酵挥发性化合物的产生。这项研究表明,从酿酒废弃物中回收利用废酒糟是可行的,并为从酿酒废弃物中提取营养物质提供了一些策略。
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引用次数: 0
Inoculation with a selected strain of Metschnikowia pulcherrima as a bioprotective alternative to sulphites for preventing browning of white grape must 接种精选的 Metschnikowia pulcherrima 菌株,作为防止白葡萄汁褐变的亚硫酸盐生物保护替代品
IF 2.9 3区 农林科学 Q1 Agricultural and Biological Sciences Pub Date : 2024-03-04 DOI: 10.20870/oeno-one.2024.58.1.7830
Marco Bustamante, P. Giménez, Arnau Just-Borràs, Ignasi Solé-Clua, Jordi Gombau, José M. Heras, N. Sieczkowski, M. Gil, J. Canals, Fernando Zamora
In this study, the effectiveness of a selected strain of Metschnikowia pulcherrima (MP1) was compared to that of sulphur dioxide for preventing white grape must from browning. Sulphur dioxide was found to drastically reduce oxygen consumption rate, protect hydroxycinnamic acids against oxidation and prevent grape must from browning, even in the presence of laccase. By contrast, supplementation with the selected strain of Metschnikowia pulcherrima (MP1) drastically increased oxygen consumption rate, thus reducing the amount of oxygen available for polyphenol oxidases. In the absence of laccase, this resulted in a decrease in browning and a certain degree of protection of the hydroxycinnamic acids, but in the presence of laccase it was not effective enough. Consequently, the selected strain of Metschnikowia pulcherrima (MP1) can be considered an interesting alternative to sulphur dioxide for preventing browning in white grape must, but only in conditions of healthy grapes.
在这项研究中,比较了所选的 Metschnikowia pulcherrima 菌株(MP1)和二氧化硫在防止白葡萄汁褐变方面的功效。结果发现,即使在有漆酶存在的情况下,二氧化硫也能大大降低耗氧率,保护羟基肉桂酸不被氧化,防止葡萄汁褐变。相比之下,补充选定的 Metschnikowia pulcherrima 菌株(MP1)会大幅提高耗氧量,从而减少多酚氧化酶可利用的氧气量。在没有漆酶的情况下,这导致了褐变的减少,并在一定程度上保护了羟基肉桂酸,但在有漆酶的情况下,这就不够有效了。因此,所选的 Metschnikowia pulcherrima 菌株(MP1)可被视为二氧化硫的一种有趣替代品,用于防止白葡萄汁中的褐变,但只能在葡萄健康的条件下使用。
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引用次数: 0
A test-tube vinification method for high-throughput characterisation of the oenological and aromatic potential of white wines 高通量表征白葡萄酒酿造和芳香潜力的试管酿造法
IF 2.9 3区 农林科学 Q1 Agricultural and Biological Sciences Pub Date : 2024-02-29 DOI: 10.20870/oeno-one.2024.58.1.7698
P. Claudel, Vincent Dumas, Cécile Thibon, Grégory Lemarquis, N. Jaegli, Ana Sivsivadzé, R. Baltenweck, P. Hugueney, Éric Duchêne
The quality of wine largely depends on aroma perception, but the genetic basis explaining the variations of aroma compound concentrations in wines is still fragmentary. To unravel links between genetic variations and aroma compound variations in hundreds of genotypes, we developed a small-scale, high-throughput test-tube vinification (TTV) method capable of producing white wines that reveal the genetic potential at the scale of a single vine stock. We evaluated this method on commercial grapevine varieties (Riesling, Gewurztraminer, Chardonnay, Chasselas, Floreal, Muscat à petits grains blancs) and genotypes resulting from a bi-parental cross, covering a wide aromatic palette. The wines produced were described by usual oenological parameters and GC-MS profiling of volatile compounds. We compared the wines obtained with the TTV method to commercial wines and to wines obtained from larger fermentation volumes (5–10 L). Our results show that the TTV method is suitable to produce white wines on a very small scale, i.e., less than 100 mL and that these small-scale wines faithfully reflect the aromatic potential of the different varieties, as would larger volume methods. The proposed method is a high-throughput approach to assess the oenological potential of hundreds of grapevine genotypes from grape material harvested on a single vine. This wine-focused direct phenotyping method will pave the way for a better understanding of the genetic determinism of wine aromas, especially for molecules that are not directly present in grapes, such as volatile thiols and 1,1,6-trimethyl-1,2-dihydronaphthalene (TDN).
葡萄酒的品质在很大程度上取决于香气感知,但解释葡萄酒中香气化合物浓度变化的遗传基础仍然很不完整。为了揭示数百种基因型的遗传变异与香气化合物变化之间的联系,我们开发了一种小规模、高通量的试管酿造(TTV)方法,该方法能够酿造出白葡萄酒,在单株葡萄树的规模上揭示遗传潜力。我们在商业葡萄品种(雷司令、琼瑶浆、霞多丽、夏瑟拉、弗洛尔、小粒白麝香)和双亲杂交产生的基因型上对这种方法进行了评估,这些基因型涵盖了广泛的芳香调色板。我们通过常规酿酒参数和挥发性化合物的气相色谱-质谱分析对酿制的葡萄酒进行了描述。我们将用 TTV 法酿制的葡萄酒与商业葡萄酒以及用较大发酵量(5-10 升)酿制的葡萄酒进行了比较。我们的研究结果表明,TTV 法适用于小规模生产白葡萄酒,即小于 100 毫升,而且这些小规模生产的葡萄酒能如实反映不同品种的芳香潜力,与大容量生产的方法一样。所建议的方法是一种高通量方法,可从单株葡萄树上采收的葡萄材料中评估数百种葡萄基因型的酿酒潜力。这种以葡萄酒为重点的直接表型方法将为更好地了解葡萄酒香气的遗传决定性铺平道路,特别是对于葡萄中不直接存在的分子,如挥发性硫醇和 1,1,6-三甲基-1,2-二氢萘 (TDN)。
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OENO One
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