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Development of a Hierarchical Rate-All-That-Apply (HRATA) methodology for the aromatic characterisation of wine 葡萄酒芳香特征分级评分(HRATA)方法的开发
IF 2.9 3区 农林科学 Q3 FOOD SCIENCE & TECHNOLOGY Pub Date : 2023-02-20 DOI: 10.20870/oeno-one.2023.57.1.5531
Lea C Koenig, R. Symoneaux, Nathalie Pouzalgues, V. Cariou, E. Vigneau, C. Coulon-Leroy
Wine aromatic characterisation is generally a complex task, even for well-trained assessors. To facilitate such characterisation, aroma terms are typically arranged in some sort of hierarchical structure, such as aroma wheels. However, information about this structure is lost with existing data acquisition and treatment methods. To fill this gap, we propose a new approach, Hierarchical-Rate-All-That-Apply (HRATA), for the characterisation of products. It combines the Rate-All-That-Apply (RATA) methodology with a hierarchical structuring of general and specific attributes. The aim is first to facilitate data acquisition and, secondly, to account for the hierarchical links among attributes during data analysis. We applied an HRATA approach to the characterisation of five rosé wines by 66 subjects based on 118 hierarchically structured aromatic attributes. Using monadic evaluation, assessors were asked to select all the attributes that characterised each wine and to rate their intensity on a three-point scale. For the data analysis, an initial coding step was carried out to represent the hierarchical structure of the attributes, which also made it possible to manage a large amount of non-evaluated data. After that, statistical tests and multivariate analyses were tailored for both the identification of discriminating attributes and the determination of a product map. Finally, the characterisation obtained with HRATA was compared to the results obtained from a descriptive analysis (DA) conducted by a trained panel. HRATA represents an interesting alternative for obtaining aromatic characterisation using a panel of subjects without collective common training or with diverse skill sets.
葡萄酒的芳香特征通常是一项复杂的任务,即使对训练有素的评估师也是如此。为了方便这样的特征,香气术语通常被安排在某种层次结构中,比如香气轮。然而,关于这种结构的信息在现有的数据采集和处理方法中丢失了。为了填补这一空白,我们提出了一种新的方法,分层率-全部适用(HRATA),用于产品的表征。它将Rate-All-That-Apply (RATA)方法与一般属性和特定属性的分层结构相结合。其目的首先是为了方便数据采集,其次是为了在数据分析期间解释属性之间的层次联系。我们基于118个层次结构的芳香属性,应用HRATA方法对66名受试者的5种玫瑰红葡萄酒进行了表征。使用一元评价法,评审员被要求选择每款葡萄酒的所有特征,并以三分制对其强度进行评级。对于数据分析,进行了初始编码步骤来表示属性的层次结构,这也使得管理大量未评估的数据成为可能。之后,针对鉴别属性的识别和产品图的确定,进行了统计测试和多变量分析。最后,将HRATA获得的特征与经过训练的小组进行的描述性分析(DA)获得的结果进行比较。HRATA代表了一种有趣的替代方法,即使用一组没有集体共同培训或具有不同技能集的受试者来获得芳香特征。
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引用次数: 1
Microbiome in soils of Mendoza: microbial resources for the development of agroecological management in viticulture 门多萨土壤微生物组:葡萄栽培农业生态管理发展的微生物资源
IF 2.9 3区 农林科学 Q3 FOOD SCIENCE & TECHNOLOGY Pub Date : 2023-02-20 DOI: 10.20870/oeno-one.2023.57.1.5585
Marcos Paolinelli, L. Martinez, S. García-Lampasona, Camilo Diaz-Quirós, Marcelo J Belmonte, Gastón Ahumada, Miguel Ángel Pirrone, M. Farber, Georgina Escoriaza, V. Longone, M. González, C. Lerena, M. Combina, L. Mercado
Rhizosphere microorganisms are considered an extension of plants, representing critical actors involved in the promotion of plant nutrient intake from the surrounding environment. Consequently, a great focus is being made on soil microorganisms since they are considered a promising source for crop resilience improvements under a global climate change scenario. To explore bacterial and fungal communities from arid soils in vineyards and their surroundings from two regions with very different climate and tillage histories, an amplicon sequencing analysis was performed. Specifically, Santa Rosa (SR) is in a region commonly known as the first zone, characterised by low altitude (607 m.a.s.l., Winkler V), while Gualtallary (G) is in the Uco Valley Zone, a region with high altitude (1245 m.a.s.l., Winkler III); both in the productive wine region of Mendoza. SR is characterised by its long cultivation history, while G is a recently cultivated region. Topsoil samples were collected and used for bacterial and fungal community profile characterisation. Ascomycota was the predominant phylum (38–97 %) in mycobiome composition, whereas Proteobacteria was the most abundant bacterial phylum (26–34 %) in both regions. Moreover, the main factor explaining microbiome differences between regions was the carbon-to-nitrogen ratio. Anaerolineae and Gammaproteobacteria were a distinctive bacterial class in SR-cultivated soils. Azospirillales were highly abundant in SR uncultivated soils, while Rhizobiales were differentially abundant in G uncultivated soils. Regarding functional analysis, soils from SR showed a higher denitrification activity of nitrifiers as well as glucose-related metabolism, while in G soils, bacterial photosynthesis activities were a differential trait. In addition, Actinobacteria abundance was lower in SR-cultivated soils, indicating a higher susceptibility of this phylum to grapevine crop practices. These results allow the development of hypothetical models of the local microbial resources and their contribution to grapevine nutrition, which is highly important to elaborate recommendations for grapevine management to preserve soil health in vine areas of Mendoza.
根际微生物被认为是植物的延伸,代表着促进植物从周围环境中吸收营养的关键因素。因此,人们非常关注土壤微生物,因为在全球气候变化的情况下,土壤微生物被认为是提高作物恢复力的一个有前途的来源。为了探索两个气候和耕作历史截然不同的地区葡萄园及其周围干旱土壤中的细菌和真菌群落,进行了扩增子测序分析。具体而言,Santa Rosa(SR)位于一个通常被称为第一区的地区,其特征是低海拔(607 m.a.s.l.,Winkler V),而Gualtallary(G)位于Uco Valley区,一个高海拔(1245 m.a.s.l.,Winkleer III);两者都位于门多萨出产葡萄酒的地区。SR的特点是其悠久的种植历史,而G是最近种植的地区。采集表层土样品,并用于细菌和真菌群落特征。子囊菌门是真菌生物群落组成中的主要门(38-97%),而变形菌门是这两个地区最丰富的细菌门(26-34%)。此外,解释地区间微生物组差异的主要因素是碳氮比。Anaerolineae和Gammaproteobacteria是SR栽培土壤中一个独特的细菌类群。氮螺旋菌在SR未开垦土壤中含量较高,而根际菌在G未开垦土壤上含量不同。在功能分析方面,SR土壤表现出较高的硝化菌反硝化活性和葡萄糖相关代谢,而在G土壤中,细菌光合作用活性是一个差异特征。此外,放线菌在SR栽培土壤中的丰度较低,表明该门对葡萄作物实践的易感性较高。这些结果允许开发当地微生物资源及其对葡萄藤营养的贡献的假设模型,这对于制定葡萄藤管理建议以保护门多萨葡萄藤地区的土壤健康非常重要。
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引用次数: 0
Exploring future changes in synchrony between grapevine (Vitis vinifera) and its major insect pest, Lobesia botrana 探讨葡萄及其主要害虫葡萄叶虫(Lobesia botrana)在同步性方面的未来变化
IF 2.9 3区 农林科学 Q3 FOOD SCIENCE & TECHNOLOGY Pub Date : 2023-02-13 DOI: 10.20870/oeno-one.2023.57.1.7250
V. Castex, I. García de Cortázar-Atauri, M. Beniston, J. Moreau, M. Semenov, M. Stoffel, P. Calanca
The European grapevine moth (Lobesia botrana) is one of the major pests of the grapevine (Vitis vinifera) in Europe. The phenology of both the insect pest and the plant has already changed over the last decades in response to rising temperatures, with a tendency towards an earlier development. The impact of a warming climate, among other factors, could alter matches in phenology between two trophic levels, being either beneficial or detrimental to V. vinifera. As a consequence, when considering a European latitudinal transect, the changes toward synchrony or a mismatch are not fully understood. In this study, we applied the results of sequential models to simulate the phenological development of V. vinifera from dormancy to physiological maturity of Chardonnay or a similar grape variety. Likewise, we simulated the phenology of L. botrana with a process-based voltinism model. Both models were calibrated and validated in previous studies. The present study aims at simulating the future evolution of both trophic levels under changing climatic conditions at four representative European locations by using quasi-transient climate scenarios up to the year 2100 that consider the RCP4.5 and RCP8.5 greenhouse-gas forcing pathways. Although some physiological adaptations could alter these results, simulations of synchrony under climate change are crucial for the adaptation of grape cultivation and varieties. This modelling work seeks to improve our understanding of the probable shifts in the timing and spatial distribution of the plant-insect interactions in a warmer climate and how this may impact their synchrony. A risk index of damage has been implemented for the different sites and greenhouse gas forcing trajectories. Results suggest an increasing damage risk for V. vinifera close to the timing of harvests in northern Europe. They also point to increasing mortality rates of the fourth generation of L. botrana in southern Europe, where temperatures will increasingly reach the upper thermal limit for insect development.
欧洲葡萄蛾(Lobesia botrana)是欧洲葡萄(Vitis vinifera)的主要害虫之一。在过去的几十年里,害虫和植物的酚学都已经随着温度的升高而发生了变化,并有提前发展的趋势。气候变暖的影响,以及其他因素,可能会改变两个营养级之间的酚学匹配,对葡萄有利或有害。因此,在考虑欧洲纬向样带时,对同步性或不匹配性的变化还没有完全理解。在本研究中,我们应用序列模型的结果来模拟霞多丽或类似葡萄品种从休眠到生理成熟的葡萄酚学发育。同样,我们用基于过程的voltinism模型模拟了L.botrana的表型。这两个模型都在之前的研究中进行了校准和验证。本研究旨在通过使用截至2100年的准瞬态气候情景,模拟四个具有代表性的欧洲地区在气候条件变化下两种营养水平的未来演变,其中考虑了RCP4.5和RCP8.5温室气体强迫途径。尽管一些生理适应可能会改变这些结果,但模拟气候变化下的同步性对葡萄种植和品种的适应至关重要。这项建模工作旨在提高我们对气候变暖时植物与昆虫相互作用的时间和空间分布可能发生的变化的理解,以及这可能如何影响它们的同步性。已经为不同的地点和温室气体排放轨迹实施了损害风险指数。研究结果表明,在北欧接近收获季节时,葡萄的损害风险正在增加。他们还指出,第四代L.botrana在南欧的死亡率不断上升,那里的温度将越来越多地达到昆虫发育的温度上限。
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引用次数: 1
A systemic approach to grapevine decline diagnosed using three key indicators: plant mortality, yield loss and vigour decrease 使用三个关键指标诊断葡萄藤衰退的系统方法:植株死亡,产量损失和活力下降
IF 2.9 3区 农林科学 Q3 FOOD SCIENCE & TECHNOLOGY Pub Date : 2023-01-27 DOI: 10.20870/oeno-one.2023.57.1.5575
A. Merot, Guillaume Coulouma, N. Smits, Elsa Robelot, C. Gary, L. Guerin-Dubrana, Jouanel Poulmach, Xavier Burgun, A. Pellegrino, M. Fermaud
Grapevine decline, a major global viticulture issue, is defined as a multi-year decrease in vine productivity and/or increase in vine mortality. Although grapevine trunk diseases are one of the most-studied causes, decline is multifactorial and associated with more than 70 factors, including abiotic and biotic hazards. With so many factors to consider, the phenomenon difficult to understand, especially for winegrowers. Our study aims to make it easier to determine and assess grapevine decline by focusing on three key indicators: yield, mortality and vegetative vigour. We investigated the relationships between these indicators from both a temporal and spatial perspective to propose a set of diagnostic indicators. Thus, we conducted a winegrowers’ survey, an historical analysis of grapevine decline and field measurements of the abovementioned indicators on plot networks in three major French winegrowing regions (see graphical abstract): Bordeaux, Cognac and Languedoc. We found that farmers’ perceptions of decline were consistent with an objective characterisation of decline based on in-field measurements of the indicators. Although vine mortality progressively spread over the years, neither the survey nor the historical analysis showed a direct link between decline and yield loss. Rather, large yearly fluctuations in yield, which did not systematically decrease over time, account for this finding. As a result, the mortality rate and the normalised difference vegetation index (NDVI) indicators were shown to be earlier indicators of grapevine decline than yield loss (yield achievement ratio, YAR). We performed a multifactorial analysis of the overall data set from the three regions to deepen our understanding of the variety of declining situations and the underlying environmental and management factors contributing to decline. Finally, two ground-based NDVI indicators and an image-analysis methodology using aerial photographs were proposed as easy-to-obtain indicators of grapevine decline. NDVI indicators were linearly correlated to both YAR and mortality rate. This study provides a better understanding and promising tools for early diagnosis of grapevine decline.
葡萄产量下降是全球葡萄栽培的一个主要问题,它被定义为葡萄产量的多年下降和/或葡萄死亡率的增加。尽管葡萄藤干病是研究最多的原因之一,但其衰退是多因素的,与70多种因素有关,包括非生物和生物危害。考虑到这么多的因素,这种现象很难理解,尤其是对葡萄种植者来说。我们的研究旨在通过关注三个关键指标:产量、死亡率和营养活力,使确定和评估葡萄藤衰退变得更容易。我们从时间和空间的角度研究了这些指标之间的关系,提出了一套诊断指标。因此,我们对法国三个主要葡萄酒产区(波尔多、干邑和朗格多克)的葡萄种植户进行了调查,对葡萄产量下降进行了历史分析,并对上述指标进行了实地测量。我们发现,农民对下降的看法与基于实地指标测量的下降的客观特征是一致的。尽管葡萄树死亡率逐年增加,但调查和历史分析都没有显示产量下降和产量损失之间的直接联系。相反,产量每年的大幅波动并没有随着时间的推移而系统地减少,这说明了这一发现。结果表明,死亡率和归一化植被指数(NDVI)指标是葡萄产量下降的早期指标,而不是产量损失(产量成就比,YAR)。我们对来自三个地区的整体数据集进行了多因素分析,以加深我们对各种下降情况以及导致下降的潜在环境和管理因素的理解。最后,提出了两个基于地面的NDVI指标和一种使用航空照片的图像分析方法,作为易于获得的葡萄树衰落指标。NDVI指标与年增长率和死亡率均呈线性相关。本研究为葡萄藤衰退的早期诊断提供了更好的认识和有希望的工具。
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引用次数: 1
Chitosan and its applications in oenology 壳聚糖及其在酿酒学中的应用
IF 2.9 3区 农林科学 Q3 FOOD SCIENCE & TECHNOLOGY Pub Date : 2023-01-24 DOI: 10.20870/oeno-one.2023.57.1.7262
Cristóbal Lárez Velásquez
This paper reviews the main applications of the biopolymer chitosan, the main derivative of chitin, a material usually obtained from natural sources accessible at low cost, i.e., industrial wastes from fisheries. Due to its natural origin, which confers biodegradability and biocompatibility properties, in addition to its low toxicity, chitosan has been gaining attention in numerous sectors, such as agriculture, food, medicine, pharmaceuticals, etc., including also important oenological applications due to its potential as a green alternative to the use of sulphite. Among the many applications that can be generated from these materials in the wine-making area, their use has been reported for the clarification of must; in the preparation of films for the removal of contaminants, whether organics such as ochratoxin A or inorganics such as some metal ions and their salts; the control of turbidity caused by protein precipitation; the encapsulation of yeasts of oenological interest and enzymes for the control of adverse microorganisms such as Brettanomyces; the manufacture of sensors and nanosensors for the quantification of contaminants, the quality control of starting materials and final products, the optimisation of fermentation processes, the monitoring of storage conditions, etc. As a result of this review, significant development of the applications of this material in the oenological area can be expected, especially due to the possibilities of preparing new derivatives, including the great variety of these that have been recently proposed through click reactions, as well as the growing incursion of chitosan in nanobiotechnology.
本文综述了生物聚合物壳聚糖的主要应用,壳聚糖是甲壳素的主要衍生物,通常从低成本的天然来源获得,即渔业工业废物。由于其天然来源,具有生物降解性和生物相容性,除了低毒性外,壳聚糖在农业、食品、医药、制药等众多领域也受到了关注,包括重要的酿酒应用,因为它有可能成为亚硫酸盐的绿色替代品。在酿酒领域中,这些材料可以产生许多应用,据报道,它们的用途是澄清必须;在制备用于去除污染物的膜时,无论是有机物如赭曲霉毒素A还是无机物如某些金属离子及其盐;控制蛋白质沉淀引起的浊度;具有酿酒学意义的酵母和用于控制不良微生物(如Brettaomyces)的酶的包封;用于污染物定量的传感器和纳米传感器的制造、原料和最终产品的质量控制、发酵过程的优化、储存条件的监测等。由于这一综述,预计这种材料在酿酒领域的应用将有重大发展,特别是由于制备新衍生物的可能性,包括最近通过点击反应提出的各种衍生物,以及壳聚糖在纳米生物技术中的日益渗透。
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引用次数: 0
Use of unripe grape wine as a tool for reducing alcohol content and improving the quality and oenological characteristics of red wines 使用未成熟的葡萄酒作为降低酒精含量、提高红酒质量和酿酒特性的工具
IF 2.9 3区 农林科学 Q3 FOOD SCIENCE & TECHNOLOGY Pub Date : 2023-01-24 DOI: 10.20870/oeno-one.2023.57.1.7226
A. Martínez-Moreno, P. Martínez-Pérez, A. Bautista‐Ortín, E. Gómez‐Plaza
In Mediterranean viticulture, climatic conditions are the main factors that determine grape composition and wine quality. Global warming is causing an increase in the accumulation of soluble solids in grapes, leading to early harvests that result in wines with high alcohol and low phenolic content and colour intensity. The aim of this research was to determine the effectiveness of mixing wines vinified from grapes with different maturation degrees from two consecutive vintages (2018 and 2019) in order to obtain wines with lower pH, lower alcohol content and higher phenolic compound concentration. The wine obtained after mixing wine from unripe grapes (URG) with second harvest (H2) wines significantly improved the physicochemical, chromatic and phenolic characteristics compared to the first harvest (H1) wine. On the other hand, the URGH2 wine had significantly lower alcohol content and pH and higher titratable acidity than the H2 wine. URGH2 maintained its colour intensity in both years and similar values of anthocyanin and tannin in 2018 compared to H2. All the wines were subjected to sensory analysis and the panelists were able to differentiate the wines in a triangle sensory test; the URGH2 wine was preferred in both years. These results show that the blend of unripe grape with wines from technologically mature grapes to reduce alcohol content and improve wine colour could be a useful tool for mitigating the problems caused by global warming in a warm and semiarid Mediterranean climate.
在地中海葡萄栽培中,气候条件是决定葡萄成分和葡萄酒品质的主要因素。全球变暖导致葡萄中可溶性固形物的积累增加,导致早期收获,导致葡萄酒酒精含量高,酚含量低,颜色强度低。本研究的目的是确定连续两个年份(2018年和2019年)不同成熟程度的葡萄混合酿造葡萄酒的有效性,以获得pH值更低、酒精含量更低、酚类化合物浓度更高的葡萄酒。与第一次收获(H1)葡萄酒相比,未成熟葡萄(URG)葡萄酒与第二次收获(H2)葡萄酒混合后获得的葡萄酒在物理化学,颜色和酚类特性方面显着改善。另一方面,URGH2葡萄酒的酒精含量和pH值显著低于H2葡萄酒,可滴定酸度显著高于H2葡萄酒。与H2相比,URGH2在这两年都保持了其颜色强度,2018年的花青素和单宁含量与H2相似。所有的葡萄酒都接受了感官分析,小组成员能够在三角感官测试中区分葡萄酒;在这两年中,URGH2葡萄酒都是首选。这些结果表明,将未成熟的葡萄与技术成熟的葡萄混合,以降低酒精含量并改善葡萄酒的颜色,可能是缓解全球变暖在温暖和半干旱的地中海气候中造成的问题的有用工具。
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引用次数: 0
A method to position a simple strip trial to improve trial efficiency and maximise the value of vineyard variability for decision-making 一种定位简单条形试验的方法,以提高试验效率,最大化葡萄园可变性的决策价值
IF 2.9 3区 农林科学 Q3 FOOD SCIENCE & TECHNOLOGY Pub Date : 2023-01-18 DOI: 10.20870/oeno-one.2023.57.1.5542
Xingyi Song, R. Bramley, K. Evans
The main difficulties grapegrowers and consultants face in obtaining robust trial results include time and labour to collect data and land variability that confounds trial results. Spatial approaches that use whole-field designs, sensing technologies and geostatistical analysis enable more efficient data collection and account for the impact of spatial variation on crop responses while generating statistically robust results. However, the practical application of these approaches for vineyard trials requires affordable automation of measurements of viticultural variables and access to skills for geostatistics. A strip approach has been developed to simplify experimentation by allowing the farmer to use a single crop row to trial and analyse data in a spreadsheet. However, guidance is needed as to how to position trial strips in a vineyard block to reveal likely treatment effects across the entire block. Here, we investigated using a covariate to a response variable of interest to position a strip trial to infer treatment effects beyond the trial strip. Strip trials were simulated for two experiments: one comparing three treatments for vineyard floor management on grape yield and another comparing two spray programs for powdery mildew control. Useful covariates for yield or mildew severity were determined using correlation analyses. Trial results were analysed using a moving pairwise comparison of treatments and a moving average of the covariates. Simulated trial strips that incorporated a range of variation in a useful covariate close to that encountered in the whole block showed how yield or mildew severity varied with the covariates along the strips. Importantly, such results provided information about likely crop responses in other parts of the block according to variation in the covariates, thus contributing to better-informed decision-making. Compared to whole-field approaches, this strip approach is more efficient and simpler for growers to implement.
葡萄种植者和顾问在获得可靠的试验结果方面面临的主要困难包括收集数据的时间和劳动力,以及混淆试验结果的土地变异性。使用整地设计、传感技术和地质统计分析的空间方法能够更有效地收集数据,并考虑到空间变化对作物反应的影响,同时产生统计上稳健的结果。然而,这些方法在葡萄园试验中的实际应用需要对葡萄栽培变量的测量进行负担得起的自动化,并获得地质统计学技能。已经开发了一种条形方法,通过允许农民使用单个作物行来试验和分析电子表格中的数据来简化实验。然而,需要指导如何在葡萄园区块中放置试验条,以揭示整个区块可能的治疗效果。在这里,我们研究了使用感兴趣的反应变量的协变来定位条形试验,以推断试验条形之外的治疗效果。模拟了两个试验的条带试验:一个试验比较了葡萄园地面管理对葡萄产量的三种处理,另一个试验则比较了两种喷雾方案对白粉病的控制。使用相关分析确定了产量或霉菌严重程度的有用协变量。使用治疗的移动成对比较和协变量的移动平均值来分析试验结果。模拟试验条带的有用协变量的变化范围与整个区块中遇到的协变量接近,显示了产量或霉菌严重程度如何随条带的协变量而变化。重要的是,这些结果根据协变量的变化提供了关于区块其他部分可能的作物反应的信息,从而有助于更好地进行知情决策。与整地方法相比,这种条带方法对种植者来说更有效、更简单。
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引用次数: 0
Isotopic comparison and correlation of δ13C between bulk wood and cellulose of Vitis vinifera L. 葡萄散装木材与纤维素的δ13C同位素比较及相关性研究。
IF 2.9 3区 农林科学 Q3 FOOD SCIENCE & TECHNOLOGY Pub Date : 2023-01-17 DOI: 10.20870/oeno-one.2023.57.1.5516
Federico Ariel Roig Puscama, F. Berli, B. Bois, O. Mathieu, F. Roig, P. Piccoli
This study aims to compare the δ13C isotopic signal between bulk wood and α-cellulose in wood samples from the main trunk of Vitis vinifera L. to verify whether α-cellulose extraction is necessary for ecophysiological studies in this species. A pool of samples from different cultivars and provenances was analysed. The wood samples were obtained from cross sections of the main trunk of the plants, from where the annual growth rings were anatomically recognised, dated to the year of formation, and then separated. Each ring comprised both early- and latewood portions. For each sample, a part was saved as bulk wood and another part was destined for α-cellulose extraction. The δ13C isotopic signal in both types of samples was performed on a Vario Micro Cube elemental analyser coupled to a continuous flow mode to an isotope ratio mass spectrometer. A least squares regression was used to verify the correlation between the two variables. The results showed that the correlation coefficient of the isotopic signal for both types of samples was 0.86, and the slope of the regression line was not significantly different from one. Those results indicated that it is acceptable to use bulk wood instead of α-cellulose for δ13C isotopic studies in Vitis vinifera. This study is the first to compare isotopic δ13C signals between bulk wood and α-cellulose in grapevines. Therefore, this study constitutes a starting point to explore dendrochemical techniques based on the analysis of the δ13C content in Vitis vinifera wood, with the aim of deepening the ecophysiological knowledge of the grapevine in relation to water economy strategies and the links with climate variability and change.
本研究旨在比较葡萄主干木材样品中散装木材和α-纤维素的δ13C同位素信号,以验证α-纤维素是否需要提取用于该物种的生态生理研究。对来自不同品种和种源的样品池进行了分析。木材样本是从植物主干的横截面上获得的,从解剖学上识别出年轮,确定其形成的年份,然后分离。每个环都包括早期和晚期的部分。对于每个样品,一部分作为散装木材保存,另一部分用于α-纤维素提取。两种样品的δ13C同位素信号在Vario Micro Cube元素分析仪上进行,该分析仪与同位素比值质谱仪耦合为连续流模式。使用最小二乘回归来验证两个变量之间的相关性。结果表明,两种样品的同位素信号相关系数均为0.86,回归线斜率与1无显著差异。这些结果表明,用散装木材代替α-纤维素进行葡萄δ13C同位素研究是可以接受的。本研究首次比较了葡萄藤散装木材和α-纤维素的同位素δ13C信号。因此,本研究是在葡萄木材δ13C含量分析的基础上探索树木化学技术的起点,旨在加深葡萄生态生理与水经济策略及其与气候变率和变化的联系的认识。
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引用次数: 1
Canopy management through crop forcing impacts grapevine cv. ‘Touriga Nacional’ performance, ripening and berry metabolomics profile 通过作物强迫对葡萄植株进行冠层管理。“Touriga Nacional”的性能,成熟和浆果代谢组学分析
IF 2.9 3区 农林科学 Q3 FOOD SCIENCE & TECHNOLOGY Pub Date : 2023-01-13 DOI: 10.20870/oeno-one.2023.57.1.7122
Inês L. Cabral, António Teixeira, Manon Ferrier, A. Lanoue, Joana Valente, F. Rogerson, F. Alves, Susana M. P. Carvalho, H. Gerós, J. Queiroz
Climate changes are speeding up the maturation of grapes in numerous areas of the world, including in the Mediterranean basin, but warmer temperatures often uncouple technical and phenolic maturity, resulting in unbalanced wines. We tested the efficacy of crop forcing (CF) in delaying the maturation of cv. ‘Touriga Nacional’ vines of the Douro Region, and their impacts on plant performance, berry quality attributes and metabolome were also evaluated. In two consecutive seasons (2019 and 2020), CF was conducted 15 (CF1) and 30 (CF2) days after fruit set by hedging growing shoots to five nodes and removing summer laterals, leaves and clusters. Results showed that while CF2 delayed ripening up to 51 days till first autumn rainfall, which compromised optimal sugar ripeness, CF1 delayed the technical maturation by one month, but both treatments severely impacted the production, mainly CF1, which reduced grapevine yield up to 90 %. The effect of CF in protecting vines against drought stress was not evident, judging by the values of leaf pre-dawn water potential measured along both seasons. CF1 and CF2 resulted in berries with lower pH and higher titratable acidity than controls, while total phenolics content increased by up to 48 % in 2020. A UPLC–MS-based targeted metabolomic analysis showed that CF increased the relative abundance of key metabolites like flavan-3-ols (i.e., catechin gallate increased by up to 661 %), trihydroxylated anthocyanins (i.e., delphinidin-3-O-glucoside increased by up to 656 % after CF2) and stilbenes (resveratrol increased by up to 700 % after CF2) with potential positive impacts in wine quality.
气候变化正在加速世界上许多地区的葡萄成熟,包括地中海盆地,但温度升高往往会使技术成熟度和酚类成熟度脱节,导致葡萄酒不平衡。我们测试了作物强迫(CF)对延迟cv成熟的效果。研究了杜罗河地区“Touriga Nacional”葡萄树及其对植株性能、果实品质属性和代谢组的影响。在连续两个季节(2019年和2020年),CF在坐果后15 (CF1)和30 (CF2)天进行,通过树篱将枝条生长到5节,去除夏季侧枝、叶子和簇。结果表明,CF2处理将葡萄成熟期推迟了51天,直至秋初降雨,影响了葡萄的最佳成熟期,而CF1处理将技术成熟期推迟了1个月,但两种处理都严重影响了葡萄产量,其中CF1处理影响最大,使葡萄产量降低了90%。从两个季节叶片黎明前水势的测量值来看,CF对葡萄抗干旱胁迫的保护作用并不明显。与对照相比,CF1和CF2的果实pH值较低,可滴定酸度较高,而总酚类物质含量在2020年增加了48%。一项基于ulc - ms的目标代谢组学分析显示,CF增加了关键代谢物的相对丰度,如黄烷-3-醇(即儿茶素-没食子酸酯增加了661%),三羟基花青素(即蒲草苷-3-o -葡萄糖苷增加了656%)和二苯乙烯(白藜芦醇增加了700%),对葡萄酒质量有潜在的积极影响。
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引用次数: 3
Delayed grape ripening by intermittent shading to counter global warming depends on carry-over effects and water deficit conditions 通过间歇性遮阳延缓葡萄成熟以对抗全球变暖取决于携带效应和水分亏缺条件
IF 2.9 3区 农林科学 Q3 FOOD SCIENCE & TECHNOLOGY Pub Date : 2023-01-13 DOI: 10.20870/oeno-one.2023.57.1.5521
Benjamin Tiffon-Terrade, T. Simonneau, A. Caffarra, Romain Boulord, P. Péchier, N. Saurin, Charles Romieu, Damien Fumey, A. Christophe
Grapevine phenology is continuously advancing due to global warming, exposing berry ripening to increasingly drier and hotter episodes that can dramatically affect yield and berry quality. This study aimed to analyse whether intermittent shading produced by panels placed over the plants can delay berry ripening to counter the impact of global warning on phenology. A two-year outdoor trial repeated on two batches of young potted grapevine (cv. Syrah) was conducted in Montpellier (South of France). Shading was created in a row using 2 m-wide horizontal panels placed 2.4 m above the ground. A moderate water deficit was also applied at the start of veraison to half the plants in both full sun (without panels) and shaded conditions to mimic usual field conditions. Variables related to budburst, flowering, veraison and sugar at harvest were analysed in all treatments. Although intermittent shading did not significantly modify air temperature within the canopy when cumulated over the growing season, the panels substantially delayed veraison by up to more than 30 days under well-watered conditions. The most marked phenological shifts were noted in the second year of treatment between flowering and veraison when carbon demand sharply increased during berry formation, suggesting there was a carry-over effect likely due to limited carbon assimilation. This was accompanied by sharp decreases in berry diameter and sugar content per berry at harvest. Higher berry growth and sugar loading were maintained when shading was combined with water deficit. However, the trigger effect of water deficit on veraison almost halved the phenological delay caused by the panels. Overall, a cooler period for ripening could be achieved with panels over the vines but at the expense of berry size and sugar amount in berries. It can be concluded that shading intensity and duration should be adapted to evaporative and soil water conditions to benefit from the phenological delay caused by panels, without altering production in the long term.
由于全球变暖,葡萄的酚学正在不断发展,使浆果成熟变得越来越干燥和炎热,这会极大地影响产量和浆果质量。这项研究旨在分析放置在植物上的面板产生的间歇性遮荫是否会延迟浆果成熟,以应对全球警报对酚学的影响。在蒙彼利埃(法国南部)对两批年轻的盆栽葡萄(cv.Syrah)进行了为期两年的室外试验。使用放置在离地2.4米处的2米宽的水平面板创建一排遮阳板。在veraison开始时,在阳光充足(没有面板)和遮荫条件下,对一半的植物进行了适度的缺水处理,以模拟通常的田间条件。分析了所有处理中与芽突、开花、白藜芦醇和收获糖相关的变量。尽管在生长季节积累时,间歇性遮荫不会显著改变冠层内的空气温度,但在水分充足的条件下,这些面板显著推迟了30多天的恢复期。在处理的第二年,在开花和白藜芦醇之间,当浆果形成期间碳需求急剧增加时,注意到了最显著的酚类变化,这表明可能由于碳同化有限而存在结转效应。收获时,浆果直径和每粒浆果的含糖量急剧下降。当遮荫与缺水相结合时,保持了较高的浆果生长和糖负荷。然而,缺水对白藜芦醇的触发作用几乎使面板造成的酚学延迟减半。总的来说,在葡萄藤上覆盖面板可以达到较冷的成熟期,但这要以浆果的大小和浆果中的糖分为代价。可以得出的结论是,遮荫强度和持续时间应适应蒸发和土壤水分条件,以受益于面板造成的酚性延迟,而不会长期改变生产。
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引用次数: 0
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