{"title":"The influence of various concentrations of essential oils on the growth rate of fibrolytic and amylolytic bacteria isolated from rumen fluid","authors":"Zeynep Sahan","doi":"10.30848/pjb2024-1(36)","DOIUrl":"https://doi.org/10.30848/pjb2024-1(36)","url":null,"abstract":"","PeriodicalId":19962,"journal":{"name":"Pakistan Journal of Botany","volume":" ","pages":""},"PeriodicalIF":1.2,"publicationDate":"2023-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44609575","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Switchgrass (Panicum virgatum ) is used as a fodder for livestock due to its high nutritional value; it has potential to grow in cool and warm conditions as well as in low fertile soil. Switchgrass breeding is difficult due to its high degree of self-incompatibility; hence, grass production can be increased through cell and tissue culture. The present research focused on developing protocols for the regeneration and gene transformation of the forage grass to enhance the callus and regeneration efficiency through Agrobacterium -mediated transformation. The switchgrass callus was transformed with the pCAMBIA1302 vector carrying beta-glucuronidase (GUS) reporter gene, and 42% transformation efficiency was attained. To ensure a successful transformation, explants were infected with Agrobacterium for five minutes, following co-cultivation for two days. To enhance the process, 100 µM acetosyringone was added in co-cultivation media. Pre-selection of transformed callus was carried out for seven days with 150 mg/L of cefixime. The switchgrass transgenic callus was shifted to a selection medium containing hygromycin (50 mg/L). Histochemical staining was used to evaluate the presence of the GUS gene in the hygromycin-resistant transgenic plants. The successful transformation of switchgrass opens opportunities for gene function analysis and germplasm improvement through the application of advanced biotechnological tools.
{"title":"Evaluation of parameters affecting the genetic engineering of recalcitrant switchgrass (Panicum virgatum Roxb. ex Steud.) using GUS reporter gene system","authors":"Saima Shafique, N. Jabeen, Samra Irum","doi":"10.30848/pjb2024-1(37)","DOIUrl":"https://doi.org/10.30848/pjb2024-1(37)","url":null,"abstract":"Switchgrass (Panicum virgatum ) is used as a fodder for livestock due to its high nutritional value; it has potential to grow in cool and warm conditions as well as in low fertile soil. Switchgrass breeding is difficult due to its high degree of self-incompatibility; hence, grass production can be increased through cell and tissue culture. The present research focused on developing protocols for the regeneration and gene transformation of the forage grass to enhance the callus and regeneration efficiency through Agrobacterium -mediated transformation. The switchgrass callus was transformed with the pCAMBIA1302 vector carrying beta-glucuronidase (GUS) reporter gene, and 42% transformation efficiency was attained. To ensure a successful transformation, explants were infected with Agrobacterium for five minutes, following co-cultivation for two days. To enhance the process, 100 µM acetosyringone was added in co-cultivation media. Pre-selection of transformed callus was carried out for seven days with 150 mg/L of cefixime. The switchgrass transgenic callus was shifted to a selection medium containing hygromycin (50 mg/L). Histochemical staining was used to evaluate the presence of the GUS gene in the hygromycin-resistant transgenic plants. The successful transformation of switchgrass opens opportunities for gene function analysis and germplasm improvement through the application of advanced biotechnological tools.","PeriodicalId":19962,"journal":{"name":"Pakistan Journal of Botany","volume":" ","pages":""},"PeriodicalIF":1.2,"publicationDate":"2023-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45279343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
F. Khan, Muhammad Akram, A. Rashid, Syed Muhammad Ali Shah, Sultan Ayaz
The purpose of this research is to determine the phytochemical analysis of traditional medicinal plants : Taraxacum officinale, Geranium wallichianum, and Elaeagnus parvifolia. Phytochemical screening of plants is an essential step in discovering bioactive components of medicinal plants, which might contribute to the formulation of novel medicines and improve the medications. The presence of important bioactive components in these three traditional medicinal plants commonly used in Asia has been identified to correlate with their ethnomedicinal importance. These plants include Taraxacum officinale , Geranium wallichianum , and Elaeagnus parvifolia . We used standard techniques to reveal the existence of secondary metabolites that include tannins, flavonoids, phenols, saponins, sterols, cardiac glycosides, and alkaloids. All the three species of plants showed different results. Tannins and saponins were mostly present in Taraxacum officinale . Geranium wallichianum contains most of terpenoids. Phytosterol, glycosides and carbohydrates were found in Taraxacum officinale and Geranium wallichianum. Alkaloids, saponins and flavonoids are present in all the three species therefore all of these plants have antioxidants properties and are of great medicinal use as well.
{"title":"Phytochemical screening of three traditional medicinal plants: Taraxacum officinale, Geranium wallichianum, and Elaeagnus parvifolia","authors":"F. Khan, Muhammad Akram, A. Rashid, Syed Muhammad Ali Shah, Sultan Ayaz","doi":"10.30848/pjb2024-1(39)","DOIUrl":"https://doi.org/10.30848/pjb2024-1(39)","url":null,"abstract":"The purpose of this research is to determine the phytochemical analysis of traditional medicinal plants : Taraxacum officinale, Geranium wallichianum, and Elaeagnus parvifolia. Phytochemical screening of plants is an essential step in discovering bioactive components of medicinal plants, which might contribute to the formulation of novel medicines and improve the medications. The presence of important bioactive components in these three traditional medicinal plants commonly used in Asia has been identified to correlate with their ethnomedicinal importance. These plants include Taraxacum officinale , Geranium wallichianum , and Elaeagnus parvifolia . We used standard techniques to reveal the existence of secondary metabolites that include tannins, flavonoids, phenols, saponins, sterols, cardiac glycosides, and alkaloids. All the three species of plants showed different results. Tannins and saponins were mostly present in Taraxacum officinale . Geranium wallichianum contains most of terpenoids. Phytosterol, glycosides and carbohydrates were found in Taraxacum officinale and Geranium wallichianum. Alkaloids, saponins and flavonoids are present in all the three species therefore all of these plants have antioxidants properties and are of great medicinal use as well.","PeriodicalId":19962,"journal":{"name":"Pakistan Journal of Botany","volume":" ","pages":""},"PeriodicalIF":1.2,"publicationDate":"2023-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48002098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Fresh cuttings from 2-year-old rose cultivar Rose gruss-an-teplitz was treated with 2 PGPR used as seed soaking treatment and with Zinc sulphate added to the rhizosphere soil or treated with Salicylic acid (SA) as foliar spray was sown both in the field and in pots (under sterilized condition). Physiological parameters were recorded at flowering. All the treatments promoted growth parameters and improved the quality of rose water. However, greater increase in all growth parameters were recorded in Pseudomonas putida + Salicylic acid treatment. Potted grown plants also showed significant stimulation in growth and reproductive parameters; Bacillus cereus being highly effective. The treatment effects were greater in summer but in winter the % increase was also significantly greater over control. Organoleptic analysis of rose water revealed that treatment with Pseudomonas putida + Salicylic acid (SA) imparted maximum aroma and sweetness in field grown plants while Bacillus cereus inoculation proved most effective in potted plants. Plant was more responsive to treatments in summer than that of winter. Rose petal water from potted plants of winter revealed higher aroma in winter as compared to summer. Similar trend was noted in case of field grown plants. The incidence of disease was not recorded in treated plants particularly in presence of SA. Both the PGPR and SA act synergistically and can be applied to boost growth, yield and quality of roses. This is the first report regarding the effect of PGPR, defense hormone SA and Zinc sulphate alone and in combination on roses. The investigation revealed that PGPR can augment the effectivity of Zinc sulphate and SA on growth and reproductivity of rose as well as on its quality e.g., flavor and sweetness of rose water. The combined treatments may be implicated to enhance productivity and quality of roses.
{"title":"Physiology of Rosa ‘Gruss-an-teplitz’ following treatment with rhizobacteria (PGPR), salicylic acid (SA) and zinc sulphate","authors":"Mian. M. Amanullah, A. Bano","doi":"10.30848/pjb2024-1(38)","DOIUrl":"https://doi.org/10.30848/pjb2024-1(38)","url":null,"abstract":"Fresh cuttings from 2-year-old rose cultivar Rose gruss-an-teplitz was treated with 2 PGPR used as seed soaking treatment and with Zinc sulphate added to the rhizosphere soil or treated with Salicylic acid (SA) as foliar spray was sown both in the field and in pots (under sterilized condition). Physiological parameters were recorded at flowering. All the treatments promoted growth parameters and improved the quality of rose water. However, greater increase in all growth parameters were recorded in Pseudomonas putida + Salicylic acid treatment. Potted grown plants also showed significant stimulation in growth and reproductive parameters; Bacillus cereus being highly effective. The treatment effects were greater in summer but in winter the % increase was also significantly greater over control. Organoleptic analysis of rose water revealed that treatment with Pseudomonas putida + Salicylic acid (SA) imparted maximum aroma and sweetness in field grown plants while Bacillus cereus inoculation proved most effective in potted plants. Plant was more responsive to treatments in summer than that of winter. Rose petal water from potted plants of winter revealed higher aroma in winter as compared to summer. Similar trend was noted in case of field grown plants. The incidence of disease was not recorded in treated plants particularly in presence of SA. Both the PGPR and SA act synergistically and can be applied to boost growth, yield and quality of roses. This is the first report regarding the effect of PGPR, defense hormone SA and Zinc sulphate alone and in combination on roses. The investigation revealed that PGPR can augment the effectivity of Zinc sulphate and SA on growth and reproductivity of rose as well as on its quality e.g., flavor and sweetness of rose water. The combined treatments may be implicated to enhance productivity and quality of roses.","PeriodicalId":19962,"journal":{"name":"Pakistan Journal of Botany","volume":" ","pages":""},"PeriodicalIF":1.2,"publicationDate":"2023-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47882176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Influence of different nitrogen levels on the morpho-agronomic and quality traits of some mung bean [Vigna radiata (L.) Wilczek] genotypes","authors":"Aybegün Ton","doi":"10.30848/pjb2024-1(29)","DOIUrl":"https://doi.org/10.30848/pjb2024-1(29)","url":null,"abstract":"","PeriodicalId":19962,"journal":{"name":"Pakistan Journal of Botany","volume":" ","pages":""},"PeriodicalIF":1.2,"publicationDate":"2023-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41874452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Timour Shah Zafar, Asfandyar Durrani, S. Hashmi, N. Ahmad, Waqar Ahmad, Muhammad Nadeem, B. Abbasi, Muhammad Tariq
{"title":"Elicitation of secondary metabolites and physiological changes in Ipomea turbinata and Convolvulus arvensis plantlets exposed to green synthesized silver nanoparticles","authors":"Timour Shah Zafar, Asfandyar Durrani, S. Hashmi, N. Ahmad, Waqar Ahmad, Muhammad Nadeem, B. Abbasi, Muhammad Tariq","doi":"10.30848/pjb2024-1(28)","DOIUrl":"https://doi.org/10.30848/pjb2024-1(28)","url":null,"abstract":"","PeriodicalId":19962,"journal":{"name":"Pakistan Journal of Botany","volume":" ","pages":""},"PeriodicalIF":1.2,"publicationDate":"2023-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42539856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Biosynthesis of glucoamylase from Candida lipolytica using solid state fermentation","authors":"U. Hameed, Sardar Junaid Bahadur Khan, I. Haq","doi":"10.30848/pjb2024-1(32)","DOIUrl":"https://doi.org/10.30848/pjb2024-1(32)","url":null,"abstract":"","PeriodicalId":19962,"journal":{"name":"Pakistan Journal of Botany","volume":" ","pages":""},"PeriodicalIF":1.2,"publicationDate":"2023-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45985724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
N. N. Nawab, Muhammad Kashif Shahzad Sarwar, S. K. Tanveer, Alamgir Alvi
{"title":"Augmented block design for evaluating round and oblong fruit shape advanced lines of indeterminate tomato (Solanum lycopersicum L.)","authors":"N. N. Nawab, Muhammad Kashif Shahzad Sarwar, S. K. Tanveer, Alamgir Alvi","doi":"10.30848/pjb2024-1(27)","DOIUrl":"https://doi.org/10.30848/pjb2024-1(27)","url":null,"abstract":"","PeriodicalId":19962,"journal":{"name":"Pakistan Journal of Botany","volume":" ","pages":""},"PeriodicalIF":1.2,"publicationDate":"2023-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46226838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Karyomorphology of genus Pinanga (Arecaceae) in Java and Bali, Indonesia","authors":"J. Witono, I. Fijridiyanto, K. Kondo","doi":"10.30848/pjb2024-1(30)","DOIUrl":"https://doi.org/10.30848/pjb2024-1(30)","url":null,"abstract":"","PeriodicalId":19962,"journal":{"name":"Pakistan Journal of Botany","volume":"1 1","pages":""},"PeriodicalIF":1.2,"publicationDate":"2023-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69690348","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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